CN216891015U

CN216891015U - Shake bottle

Info

Publication number: CN216891015U
Application number: CN202122859196.8U
Authority: CN
Inventors: 袁晔; 方想元; 李慧伦; 袁建华
Original assignee: Guangzhou Jet Bio Filtration Co Ltd
Current assignee: Guangzhou Jet Bio Filtration Co Ltd
Priority date: 2021-11-19
Filing date: 2021-11-19
Publication date: 2022-07-05
Anticipated expiration: 2031-11-19

Abstract

The utility model relates to the technical field of biological culture experimental equipment, and discloses a shake flask which comprises a bottle cap and a bottle body, wherein the bottle cap is detachably covered on the bottle body, and a spoiler is arranged at the bottom inside the bottle body. According to the utility model, the spoiler is arranged, so that the laminar flow of the liquid in the bottle body can be disturbed, the aeration efficiency of the liquid is improved, the oxygen dissolving capacity of the liquid in the shake flask is improved, the growth of cells is further promoted, and the survival rate and activity of the cells are improved.

Description

Shake bottle

Technical Field

The utility model relates to the technical field of biological culture experimental equipment, in particular to a shake flask.

Background

The shake flask is the simplest and most common container used in the process of culturing microorganisms, and is widely applied to culture and expansion of microorganism strains. The gas exchange and the dissolved oxygen of the shake flask are mainly obtained by air intake from a bottle mouth, and for the culture of aerobic strains and genetic engineering strains with large oxygen consumption, the insufficient dissolved oxygen easily causes the bad growth state of microbial strains, even the bad conditions of bacterial dissolution and the like. The shake flask is usually installed on a shaking table, the shaking table controls the shake flask to rotate around a central axis of the shake flask, in the process, liquid in the shake flask is in a laminar flow state, the oxygen dissolving capacity is poor, the mass transfer of oxygen is small, the liquid in the shake flask is used for culturing cells, the life activities of the cells such as growth, development, division, propagation and the like all need a large amount of oxygen for supporting, and the oxygen is insufficient to be unfavorable for the growth of the cells.

SUMMERY OF THE UTILITY MODEL

The purpose of the utility model is: provides a shake flask which can improve the oxygen dissolving capacity of liquid in the shake flask and promote the growth of cells.

In order to achieve the purpose, the utility model provides a shake flask, which comprises a flask cover and a flask body, wherein the flask cover is detachably covered on the flask body, and a spoiler is arranged at the bottom of the flask inside the flask body.

Optionally, the number of spoilers is the polylith, and the polylith the spoiler is along the center pin annular array of bottle sets up.

Optionally, the spoiler is fan-shaped, and the centre of a circle of the spoiler is towards the center pin setting of bottle.

Optionally, the circle center of the spoiler is in smooth transition.

Optionally, the spoiler with the bottle body integrated into one piece.

Optionally, a concave portion corresponding to the spoiler is arranged at the bottom of the bottle body outside the bottle body.

Optionally, the bottle cap is in threaded connection with the bottle body.

Optionally, shake the bottle, still include the sealing washer, the sealing washer set up in the bottleneck of bottle with between the bottle lid.

Optionally, the inner wall of the bottle cap is provided with a limiting block, and the sealing ring is arranged between the limiting block and the bottle mouth of the bottle body.

The utility model provides a shake flask, which has the following beneficial effects compared with the prior art:

the shake flask comprises a flask cover and a flask body, wherein the flask cover is detachably covered on the flask body, and a spoiler is arranged at the bottom of the flask inside the flask body. Through setting up the spoiler, can disturb the laminar flow of liquid in the bottle, improve the ventilation efficiency of liquid, improve the dissolved oxygen ability of shaking interior liquid of bottle, and then promote the growth of cell, improve cell survival rate and activity.

Drawings

Fig. 1 is a schematic structural diagram of an embodiment of the present invention.

Fig. 2 is a schematic structural diagram of another angle according to an embodiment of the present invention.

Fig. 3 is an exploded view of an embodiment of the present invention.

FIG. 4 is a schematic view of the internal structure of the bottle body according to the embodiment of the present invention.

Fig. 5 is a schematic structural view of a bottle cap according to an embodiment of the present invention.

In the figure, 1, a bottle cap; 2. a bottle body; 3. a seal ring; 11. a limiting block; 21. a spoiler; 22. a recessed portion.

Detailed Description

The following detailed description of embodiments of the present invention is provided in connection with the accompanying drawings and examples. The following examples are intended to illustrate the utility model but are not intended to limit the scope of the utility model.

In the description of the present invention, it should be noted that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

In addition, in the description of the present invention, "a plurality" means two or more unless otherwise specified.

The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Referring to fig. 1 to 4, a shake flask according to a preferred embodiment of the present invention includes a bottle cap 1 and a bottle body 2, the bottle cap 1 is detachably disposed on the bottle body 2, and a spoiler 21 is disposed at a bottom of the bottle body 2. Through setting up spoiler 21, can disturb the laminar flow of liquid in the bottle 2, improve the ventilation efficiency of liquid, improve the dissolved oxygen ability of shaking interior liquid of bottle, and then promote the growth of cell, improve cell survival rate and activity. The cell performance activity is stronger and the protein expression quantity is increased in the culture of mammalian cells and insect cells; for insect cells, the protein expression level is improved by 200%; for some stable cells and transient expression cells, the protein expression level can be increased by 300%. In the prior art, the oxygen dissolving capacity is usually increased by reducing the liquid in the flask or by increasing the rotational speed. In this way, the liquid in the flask is reduced, and the water inside the flask 2 is gradually evaporated during the culture process, resulting in an increase in osmotic pressure and thus inhibition of cell growth. The increased rotation speed causes the liquid in the bottle 2 to generate a higher shearing and pasting force, resulting in cell damage or cell death by dissolution. The shake flask of this embodiment, set up spoiler 21 alright improve the dissolved oxygen ability of the interior liquid of shaking the flask, need not to reduce the interior liquid of shaking the flask or improve the rotational speed, do benefit to the growth of the interior cell of bottle 2, can also save the consumption of blake bottle and culture medium.

The bottle 2 of this application compares the triangular culture bottle among the prior art, and bottle bottom department space is bigger, and the bottleneck is also wider, and the design all can improve the ventilation efficiency of liquid like this, improves the dissolved oxygen ability of shaking interior liquid of bottle.

In this embodiment, there are a plurality of spoilers 21, and the plurality of spoilers 21 are arranged in a circular array along the central axis of the bottle body 2. In this embodiment, 6 spoilers 21 are provided, the spoilers 21 are fan-shaped, and the center of the spoiler 21 is disposed toward the central axis of the bottle body 2. The center of the spoiler 21 is in smooth transition. Referring to fig. 4, the spoiler 21 is transversely disposed at the bottom of the bottle, and the edges of the spoiler 21 are smoothly transited. Set up spoiler 21 like this, can regard as the bottom of the bottle to set up uneven arch with spoiler 21 and carry out the vortex, shake the bottle and rotate the shearing force that the liquid in to bottle 2 produced lower, can shake the bottle when the dissolved oxygen ability of liquid when improving, reduce the production of foam.

In the shake flask of this embodiment, the spoiler 21 is integrally formed with the bottle body 2. A recess 22 corresponding to the spoiler 21 is provided at the bottom of the bottle 2. The integral forming is convenient for production and implementation. In the prior art, the baffle is welded on the inner wall of the bottle body 2, so that the design cost and the production cost are both high, and the problem of high cleaning difficulty is also solved in use.

In this embodiment, referring to fig. 3 and 5, the bottle cap 1 is screwed to the bottle body 2. In the process of shaking the bottle, in order to solve the growth metabolic state of the cells, the liquid in the bottle body 2 is sampled to measure the growth parameters of the cells at variable time, and the cell morphology is observed. In addition, a large amount of nutrients must be supplied to the inside of the bottle body 2 to maintain a high growth rate and high cell viability of the cells. The bottle cap 1 and the bottle body 2 are in threaded connection, so that the bottle cap 1 can be quickly opened and closed, and sampling and nutrient supplement are facilitated. The opening and closing time of the bottle cap 1 is short, the overlong separation time of the bottle cap 1 and the bottle body 2 in the sampling process can be prevented, and the risk of bacteria contamination in the bottle body 2 is reduced. The bottle cap 1 is easy to open and close, can be convenient for supplement of nutrient substances, and prevents slow growth of later-stage cells caused by insufficient nutrient supply, even tends to crack and die, and finally influences the cell density and the product expression quantity.

The shake flask of this embodiment still includes sealing washer 3, and sealing washer 3 sets up between the bottleneck of bottle 2 and bottle lid 1. The packing 3 is used to improve the sealing between the bottle cap 1 and the bottle body 2. The inner wall of the bottle cap 1 is provided with a limiting block 11, and the sealing ring 3 is arranged between the limiting block 11 and the bottle mouth of the bottle body 2. Stopper 11 can carry on spacingly to sealing washer 3, need shake a bottle because of moving the liquid and when invering, be difficult for revealing liquid from sealing washer 3.

In summary, the embodiment of the present invention provides a shake flask, which is provided with a spoiler 21, so as to disturb the laminar flow of the liquid in the flask body 2, improve the aeration efficiency of the liquid, improve the oxygen dissolving capacity of the liquid in the shake flask, further promote the growth of cells, and improve the survival rate and activity of the cells.

The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and substitutions can be made without departing from the technical principle of the present invention, and these modifications and substitutions should also be regarded as the protection scope of the present invention.

Claims

1. A shake flask is characterized by comprising a flask cover and a flask body, wherein the flask cover is detachably arranged on the flask body, and a spoiler is arranged at the bottom of the flask inside the flask body; the spoiler is fan-shaped, and the centre of a circle of the spoiler is towards the center shaft setting of bottle.

2. A shake flask according to claim 1, wherein the number of spoilers is a plurality of said spoilers being arranged in an annular array along the central axis of the flask.

3. The shake flask according to claim 2, wherein the spoiler is rounded at a center thereof.

4. The shake flask according to claim 1, wherein the spoiler is integrally formed with the flask body.

5. A shake flask according to claim 4, characterised in that a recess corresponding to the spoiler is provided at the bottom of the flask outside the flask.

6. A shake flask according to claim 1, characterised in that the cap is screwed to the flask.

7. The shake flask according to claim 1, further comprising a sealing ring disposed between the mouth of the flask body and the flask cap.

8. A shake flask according to claim 7, wherein the inner wall of the bottle cap is provided with a stopper, and the seal ring is arranged between the stopper and the mouth of the bottle body.