CN211170712U

CN211170712U - Culture apparatus for stem cell experiments

Info

Publication number: CN211170712U
Application number: CN201922091135.4U
Authority: CN
Inventors: 王蒙; 王姝洁; 王真
Original assignee: Hefei Zhongke Stem Cell Regeneration Medicine Co ltd
Current assignee: Anhui baioujing Medical Technology Co.,Ltd.
Priority date: 2019-11-27
Filing date: 2019-11-27
Publication date: 2020-08-04
Anticipated expiration: 2029-11-27

Abstract

The utility model discloses a culture apparatus for stem cell experiments, including base, container and container lid, the chassis is installed to the inboard bottom of container, and the container lid is installed to the container upper end, and the sleeve is installed to the container lid lower extreme, and the rubber pad is installed to the inboard upper end of sleeve. A temperature detection port is arranged on the container cover, a thermometer is arranged at the upper end of the temperature detection port, and a sealing shell is arranged outside the thermometer; temperature detects mouthful left side and is equipped with ventilative mouthful, and ventilative mouthful inboard is installed PTFE biomembrane, and ventilative mouthful upper end is equipped with sealed lid, and sealed lid left side is equipped with the scale, and the scale left side is equipped with the humidity and detects the mouth, and the hygrometer is installed to humidity detection mouthful upper end, and sealed shell is installed in the hygrometer outside. The utility model discloses simple structure, convenient operation can avoid cell homodisperse effectively in the container bottom, and real-time observation reaches the inside humidity and the temperature of container, can control the ventilative volume of container when the stem cell experiment.

Description

Culture apparatus for stem cell experiments

Technical Field

The utility model relates to a biological stem cell technical field specifically is a culture apparatus for stem cell experiments.

Background

Stem cells are a type of pluripotent cells that have the ability to self-replicate. Under certain conditions, it can differentiate into a variety of functional cells. The stem cells are divided into embryonic stem cells and adult stem cells according to the development stage of the stem cells. The stem cells are classified into three categories according to their developmental potential: totipotent, pluripotent and unipotent (multipotent). The stem cells are not fully differentiated and immature cells, have potential functions of regenerating various tissues, organs and human bodies, are called as universal cells in the medical field, are generally uniformly dispersed at the bottom of a container in the conventional flat-bottom stem cell culture bottle and are not beneficial to forming neurospheres; in static culture, cells are deposited in multiple layers at the center of the bottom of the container, so that nutrition and respiratory metabolism of the cells at the bottom layer are not facilitated.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a culture apparatus for stem cell experiments to solve the problem that proposes among the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme:

the utility model provides a culture apparatus for stem cell experiments, includes base, container and container lid, the container is installed to the base upper end, and the chassis is installed to the inboard bottom of container, and the container lid is installed to the container upper end, and the sleeve is installed to the container lid lower extreme, and the rubber pad is installed to the inboard upper end of sleeve, and the container outside upper end is connected to the inboard lower extreme of sleeve.

A temperature detection port is formed in the container cover, a thermometer is mounted at the upper end of the temperature detection port, and a sealing shell is mounted on the outer side of the thermometer; temperature detects mouthful left side and is equipped with ventilative mouthful, and ventilative mouthful inboard is installed PTFE biomembrane, and ventilative mouthful upper end is equipped with sealed lid, and sealed lid left side is equipped with the scale, and the scale left side is equipped with the humidity and detects the mouth, and the hygrometer is installed to humidity detection mouthful upper end, and sealed shell is installed in the hygrometer outside.

As a further aspect of the present invention: the chassis is circular, the cross section of the chassis is arc-shaped, and the height of the edge of the chassis is greater than the height of the middle of the chassis.

As a further aspect of the present invention: the inner side of the sleeve and the outer side of the upper end of the container are both provided with threads, and the inner side of the sleeve is meshed and connected with the upper end of the outer side of the container.

As a further aspect of the present invention: the rubber pad is circular in shape and is arranged between the top end of the container and the lower end of the container cover.

As a further aspect of the present invention: the sealed shell is equipped with two, installs in the hygrometer and the thermometer outside.

As a further aspect of the present invention: the sealing cover is in concave-convex connection with the ventilation opening, and the length of the sealing cover is the same as that of the ventilation opening.

As a further aspect of the present invention: the base and the sealing cover are both circular in shape, and the diameter of the base is the same as that of the sealing cover.

Compared with the prior art, the beneficial effects of the utility model are that:

the section of the bottom plate of the container bottom in the culture device for the stem cell experiment is arc-shaped, so that cells are prevented from being uniformly dispersed at the bottom of the container, and formation of neurospheres is facilitated; a rubber pad is arranged between the top end of the container and the container cover, so that a gap can be effectively avoided, and a good sealing environment is formed; the container cover is provided with a thermometer and a hygrometer, so that the humidity and temperature changes in the container can be observed conveniently, and the PTFE tissue culture membrane can prevent bacteria from entering the container when air circulation is carried out; the sealed lid of ventilative mouthful upper end can effectual control get into the inside air quantity of container, can accurately control ventilative volume according to the scale on the left side of sealed lid. The utility model discloses simple structure, convenient operation can avoid cell homodisperse effectively in the container bottom, and real-time observation reaches the inside humidity and the temperature of container, and the ventilative volume of container is controlled when can testing stem cells, can also avoid bacterium, viral pollution stem cells.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

Fig. 2 is a schematic structural view of the upper end of the container cover of the present invention.

Fig. 3 is a schematic structural view of the container and the sleeve according to the present invention.

Fig. 4 is a schematic structural diagram of embodiment 2 of the present invention.

In the figure: 1-base, 2-container, 3-base plate, 4-container cover, 5-sleeve, 6-temperature detection port, 7-thermometer, 8-humidity detection port, 9-hygrometer, 10-ventilation port, 11-sealing cover, 12-graduated scale, 13-rubber pad, 14-sealing shell and 15-PTFE tissue culture membrane.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Example 1

Referring to fig. 1 to 3, in an embodiment of the present invention, a culture apparatus for stem cell experiments includes a base 1, a container 2 and a container cover 4, the container 2 is installed on the upper end of the base 1, a chassis 3 is installed at the bottom end of the inner side of the container 2, the chassis 3 is circular, the cross section of the chassis 3 is arc-shaped, and the height of the edge of the chassis 3 is greater than the height of the middle of the chassis 3; the container cover 4 is arranged at the upper end of the container 2, the sleeve 5 is arranged at the lower end of the container cover 4, the rubber pad 13 is arranged at the upper end of the inner side of the sleeve 5, and the rubber pad 13 can effectively avoid gaps and form a good sealing environment; the lower end of the inner side of the sleeve 5 is connected with the upper end of the outer side of the container 2, threads are arranged on the inner side of the sleeve 5 and the outer side of the upper end of the container 2, and the inner side of the sleeve 5 is connected with the upper end of the outer side of the container 2 in a meshed mode.

A temperature detection port 6 is formed in the container cover 4, a thermometer 7 is mounted at the upper end of the temperature detection port 6, and a sealing shell 14 is mounted on the outer side of the thermometer 7; a ventilation opening 10 is formed in the left side of the temperature detection opening 6, a PTFE (polytetrafluoroethylene) membrane assembly 15 is arranged on the inner side of the ventilation opening 10, a sealing cover 11 is arranged at the upper end of the ventilation opening 10, the sealing cover 11 is in concave-convex connection with the ventilation opening 10, and the length of the sealing cover 11 is the same as that of the ventilation opening 10; 11 left sides of sealed lid are equipped with

scale

12, and 12 left sides of scale are equipped with humidity and detect mouth 8, and humidity detects 8 upper ends of mouth and installs hygrometer 9, and sealed shell 14 is installed in the hygrometer 9 outside, and the shape of base 1 and sealed lid 11 all is circular, and the diameter of base 1 is the same with the diameter of sealed lid 11, and sealed lid 11 of ventilative mouthful 10 upper end can the inside air quantity of effectual control entering container 2, can come accurate control ventilation volume according to the left scale 12 of sealed lid 11.

Example 2

Referring to fig. 4, in an embodiment of the present invention, a culture apparatus for stem cell experiments, includes a base 1, a container 2 and a container cover 4, the container 2 is installed at the upper end of the base 1, a chassis 3 is installed at the bottom end of the inner side of the container 2, the container cover 4 is installed at the upper end of the container 2, a sleeve 5 is installed at the lower end of the container cover 4, a rubber pad 13 is installed at the inner side of the sleeve 5, the sleeve 5 and the upper end of the container 2 are in concave-convex connection, the rubber pad 13 is installed between the sleeve 5 and the container 2, the container cover 4 can be conveniently opened and closed to the container 2. The other structure in this embodiment is the same as that in embodiment 1.

The utility model discloses a theory of operation is:

the section of a chassis 3 at the bottom end of a container 2 in the culture device for the stem cell experiment is arc-shaped, so that cells are prevented from being uniformly dispersed at the bottom of the container, and formation of neurospheres is facilitated; a rubber pad 13 is arranged between the top end of the container 2 and the container cover 4, so that gaps can be effectively avoided, and a good sealing environment is formed; the container cover 4 is provided with a thermometer 7 and a hygrometer 9, so that the humidity and temperature changes in the container 2 can be observed conveniently, and the PTFE tissue culture membrane 15 can prevent bacteria from entering the container 2 during air circulation; the sealing cover 11 at the upper end of the ventilation opening 10 can effectively control the air quantity entering the container 2, and the ventilation quantity can be accurately controlled according to the graduated scale 12 at the left side of the sealing cover 11. The utility model discloses simple structure, convenient operation can avoid cell homodisperse effectively in 2 bottoms of container, and real-time inside humidity and the temperature of observing 2 containers can be controlled the ventilative volume of container 2 when can testing stem cells, can also avoid bacterium, viral pollution stem cells.

It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims

1. A culture apparatus for stem cell experiments comprises a base (1), a container (2) and a container cover (4), wherein the container (2) is installed at the upper end of the base (1), the container cover (4) is installed at the upper end of the container (2), and the culture apparatus is characterized in that a temperature detection port (6) is arranged on the container cover (4), a thermometer (7) is installed at the upper end of the temperature detection port (6), and a sealing shell (14) is installed at the outer side of the thermometer (7); temperature detects mouthful (6) left side and is equipped with ventilative mouthful (10), and PTFE film forming (15) are installed to ventilative mouthful (10) inboard, and ventilative mouthful (10) upper end is equipped with sealed lid (11), and sealed lid (11) left side is equipped with scale (12), and scale (12) left side is equipped with humidity and detects mouthful (8), and hygrometer (9) are installed to humidity detection mouthful (8) upper end, and sealed shell (14) are installed in hygrometer (9) outside.

2. The experimental culture device for stem cells as claimed in claim 1, wherein the bottom of the inner side of the container (2) is provided with a chassis (3), the lower end of the container cover (4) is provided with a sleeve (5), the upper end of the inner side of the sleeve (5) is provided with a rubber gasket (13), and the lower end of the inner side of the sleeve (5) is connected with the upper end of the outer side of the container (2).

3. The experimental stem cell culture apparatus according to claim 2, wherein the shape of the bottom plate (3) is circular, the cross section of the bottom plate (3) is arc-shaped, and the height of the edge of the bottom plate (3) is greater than the height of the middle of the bottom plate (3).

4. The experimental stem cell culture device of claim 3, wherein the inner side of the sleeve (5) and the outer side of the upper end of the container (2) are provided with threads, and the inner side of the sleeve (5) is engaged with the upper end of the outer side of the container (2).

5. The experimental stem cell culture apparatus of claim 4, wherein the rubber mat (13) is circular in shape, and the rubber mat (13) is installed between the top end of the container (2) and the lower end of the container cover (4).

6. The experimental stem cell culture apparatus according to claim 4, wherein the two sealed housings (14) are installed outside the hygrometer (9) and the thermometer (7).

7. The experimental stem cell culture apparatus according to claim 6, wherein the sealing cap (11) and the ventilation port (10) are connected in a concave-convex manner, and the length of the sealing cap (11) is the same as the length of the ventilation port (10).

8. The experimental stem cell culture apparatus of claim 7, wherein the base (1) and the sealing cap (11) are both circular in shape, and the diameter of the base (1) is the same as that of the sealing cap (11).