CN205295359U - Closed cell culture board - Google Patents
Closed cell culture board Download PDFInfo
- Publication number
- CN205295359U CN205295359U CN201521003762.3U CN201521003762U CN205295359U CN 205295359 U CN205295359 U CN 205295359U CN 201521003762 U CN201521003762 U CN 201521003762U CN 205295359 U CN205295359 U CN 205295359U
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- CN
- China
- Prior art keywords
- upper cover
- sacculus
- cover plate
- tissue culture
- culture plate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a closed cell culture board, a serial communication port, including the upper cover plate, lap and trade the liquid device down, the upper cover plate with the apron coordinates closed cavity of formation down, it includes the sacculus and trades the liquid pipe to trade the liquid device, it passes the upper cover plate and extends down apron bottom to trade the liquid pipe, the sacculus cup joints and leaks above the upper cover plate outside and in trading liquid rectal cauda end, the sacculus is can repeated puncture sacculus. Adopt the utility model discloses, structural design is simple, equitable, and the cost of manufacture is low, has both guaranteed the gas permeability of cell culture board, makes things convenient for taking or moving of cell culture board again, still can reduce and train the risk that the cell coat pollutes in the board.
Description
Technical field
This utility model relates to a kind of cell culture apparatus tool, particularly relates to a kind of closed Tissue Culture Plate.
Background technology
Tissue Culture Plate is a kind of cell culture vessel commonly used in cell experiment, its material is generally plastics, it mostly is single use, but currently marketed Cell culture plate structure not links closely between lid and ware body, the lid in liquid process that changes at Tissue Culture Plate is frequently opened, and causes that extraneous antibacterial etc. enters cell culture well and causes cell contamination.
Summary of the invention
Technical problem to be solved in the utility model is in that, a kind of closed Tissue Culture Plate is provided, structural design is simple, reasonable, cost of manufacture is low, neither change the breathability of Tissue Culture Plate, facilitate again taking or mobile of Tissue Culture Plate, also can reduce the Tissue Culture Plate of the contaminated risk of cell in culture plate.
In order to solve above-mentioned technical problem, this utility model provides a kind of closed Tissue Culture Plate, it is characterised in that including upper cover plate, lower cover and liquid changing device, described upper cover plate and described lower cover cooperatively form a closed cavity; Described liquid changing device includes sacculus and change liquid pipe, described in change liquid pipe and extend bottom lower cover through upper cover plate, described sacculus is socketed on and changes liquid pipe tail end and leak outside above upper cover plate, and described sacculus is repeatable puncture sacculus.
As the improvement of technique scheme, in the middle part of described upper cover plate, it is provided with the air-vent of multiple equidistant arrangement.
As the improvement of technique scheme, described air-vent is covered with filter membrane.
As the improvement of technique scheme, described upper cover plate is connected with lower cover interference fit.
As the improvement of technique scheme, bottom described lower cover, it is coated with biomolecule.
Preferably, described biomolecule is poly-D-lysine or rat-tail glue.
Implement the closed Tissue Culture Plate of this utility model, compared with prior art, have the advantages that
(1) this utility model adopts and closed changes liquid design, it is to avoid the lid in liquid process that changes at Tissue Culture Plate is frequently opened, and causes that extraneous antibacterial etc. enters Tissue Culture Plate and causes cell contamination;
(2) this utility model adopts repeatable puncture sacculus and changes liquid pipe fit structure, improves the service life of Tissue Culture Plate;
(3) this utility model breather hole structure by film covering type, improves the breathability of Tissue Culture Plate, and not easily by the germ contamination in outside air.
Accompanying drawing explanation
In order to be illustrated more clearly that the technical scheme of this utility model embodiment, the accompanying drawing of embodiment will be briefly described below.
Fig. 1 is the structural representation of a kind of closed Tissue Culture Plate of this utility model.
Detailed description of the invention
Below in conjunction with the accompanying drawing in this utility model embodiment, the technical scheme in this utility model embodiment is clearly and completely described, it is clear that described embodiment is only a part of embodiment of this utility model, rather than whole embodiments. Based on the embodiment in this utility model, the every other embodiment that those of ordinary skill in the art obtain under not making creative work premise, broadly fall into the scope of this utility model protection.
As it is shown in figure 1, an embodiment of the present utility model, a kind of closed Tissue Culture Plate, including upper cover plate 1, lower cover 2 and liquid changing device, described upper cover plate 1 cooperatively forms a closed cavity with described lower cover 2; Described liquid changing device includes sacculus 3 and changes liquid pipe 4, described in change liquid pipe 4 and extend bottom lower cover 2 through upper cover plate 1, described sacculus 3 is socketed on and changes liquid pipe 4 tail end and leak outside above upper cover plate 1, and described sacculus 3 is repeatable puncture sacculus.
When being embodied as, experimenter places cell and culture fluid and cultivates on lower cover 2, then upper cover plate 1 and lower cover 2 adopt interference fit to connect the cavity forming a closing, when needing to change culture fluid, sterilization leaks outside behind sacculus 3 surface of upper cover plate 1, use syringe Direct perforantes sacculus 3 to carry out cell change liquid by changing liquid pipe 4, reduce cell contamination.
More preferably, the air-vent 5 of multiple equidistant arrangement it is provided with in the middle part of upper cover plate 1, it is ensured that cell cultivates required air conditions. Air-vent 5 is covered with filter membrane, it is prevented that the antibacterial in outside air enters culture plate internal contamination cell and culture fluid.
More preferably, being coated with biomolecule 6 bottom described lower cover 2, this biomolecule 6 is preferably poly-D-lysine or rat-tail glue, can increase adherent property and the activity of attached cell.
Above disclosed it is only preferred embodiment of the present utility model, certainly can not limit the interest field of this utility model, the equivalent variations therefore made according to this utility model claim with this, still belong to the scope that this utility model is contained.
Claims (6)
1. a closed Tissue Culture Plate, it is characterised in that including upper cover plate, lower cover and liquid changing device, described upper cover plate and described lower cover cooperatively form a closed cavity; Described liquid changing device includes sacculus and change liquid pipe, described in change liquid pipe and extend bottom lower cover through upper cover plate, described sacculus is socketed on and changes liquid pipe tail end and leak outside above upper cover plate, and described sacculus is repeatable puncture sacculus.
2. closed Tissue Culture Plate as claimed in claim 1, it is characterised in that be provided with the air-vent of multiple equidistant arrangement in the middle part of described upper cover plate.
3. closed Tissue Culture Plate as claimed in claim 2, it is characterised in that described air-vent is covered with filter membrane.
4. closed Tissue Culture Plate as claimed in claim 1, it is characterised in that described upper cover plate is connected with lower cover interference fit.
5. closed Tissue Culture Plate as claimed in claim 1, it is characterised in that be coated with biomolecule bottom described lower cover.
6. closed Tissue Culture Plate as claimed in claim 5, it is characterised in that described biomolecule is poly-D-lysine or rat-tail glue.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521003762.3U CN205295359U (en) | 2015-12-04 | 2015-12-04 | Closed cell culture board |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521003762.3U CN205295359U (en) | 2015-12-04 | 2015-12-04 | Closed cell culture board |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205295359U true CN205295359U (en) | 2016-06-08 |
Family
ID=56436812
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201521003762.3U Expired - Fee Related CN205295359U (en) | 2015-12-04 | 2015-12-04 | Closed cell culture board |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205295359U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109415677A (en) * | 2016-06-21 | 2019-03-01 | 株式会社三博特 | Container is used in cell/bio-tissue conveying |
-
2015
- 2015-12-04 CN CN201521003762.3U patent/CN205295359U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109415677A (en) * | 2016-06-21 | 2019-03-01 | 株式会社三博特 | Container is used in cell/bio-tissue conveying |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160608 Termination date: 20191204 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |