CN210103939U - Cell mar experimental apparatus - Google Patents
Cell mar experimental apparatus Download PDFInfo
- Publication number
- CN210103939U CN210103939U CN201920745455.4U CN201920745455U CN210103939U CN 210103939 U CN210103939 U CN 210103939U CN 201920745455 U CN201920745455 U CN 201920745455U CN 210103939 U CN210103939 U CN 210103939U
- Authority
- CN
- China
- Prior art keywords
- adsorption
- blocks
- culture dish
- block
- adsorption part
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a cell scratch experimental device, which comprises an adsorption part I, a culture dish and an adsorption part II, wherein the culture dish is placed on the adsorption part II, and the adsorption part I is placed in the culture dish and is tightly attached to the inner side surface of the bottom wall of the culture dish through being matched with the adsorption part II; the first adsorption part comprises a plurality of first adsorption blocks which are arranged at intervals. According to the experimental device, by an adsorption method, when cells are cultured in the culture dish by matching the adsorption block I and the adsorption block II, cell deletion areas are caused, so that the uniformity and the uniformity of the scratches are ensured, and the repeatability and the accuracy of a scratch experiment are achieved.
Description
Technical Field
The utility model relates to a relevant technical field of chemical instrumentation, concretely relates to cell mar experimental apparatus.
Background
The cell scratch test is an in vitro test method for observing cell migration. The method of the experiment is that cells are inoculated on a culture dish, after the cells grow full, the cell culture dish is scratched through a gun head, a transverse line or a vertical line of cell deletion is formed on the cell culture dish, and then the migration distance of the cells to the deleted area is observed. However, in the conventional experimental method, because an experimental operator automatically adopts the gun head to scratch, lines are often different in width and often skew, so that the repeatability and the accuracy of a scratch experiment are reduced.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the technical problem that to prior art not enough, provide a cell mar experimental apparatus.
The utility model provides an above-mentioned technical problem's technical scheme as follows: a cell scratch experiment device comprises a first adsorption part, a culture dish and a second adsorption part, wherein the culture dish is placed on the second adsorption part, and the first adsorption part is placed in the culture dish and tightly attached to the inner side surface of the bottom wall of the culture dish through being matched with the second adsorption part; the first adsorption part comprises a plurality of first adsorption blocks which are arranged at intervals.
The utility model has the advantages that: according to the experimental device, by an adsorption method, when cells are cultured in the culture dish by matching the adsorption block I and the adsorption block II, cell deletion areas are caused, so that the uniformity and the uniformity of the scratches are ensured, and the repeatability and the accuracy of a scratch experiment are achieved.
On the basis of the technical scheme, the utility model discloses can also do following improvement.
Furthermore, a plurality of the first adsorption blocks are connected with each other through connecting rods respectively, or a plurality of the first adsorption blocks are fixed on the connecting plates respectively.
The beneficial effect of adopting the further scheme is that: the first adsorption blocks are connected with each other or fixed on the connecting plate through the connecting rods, and the first adsorption blocks are convenient to take and place at one time.
Further, a handle is connected to the connecting rod or the connecting plate.
The beneficial effect of adopting the further scheme is that: and a handle is connected on the connecting rod or the connecting plate, so that the first adsorption block can be conveniently taken and placed by experimenters.
Further, the first adsorption block is a magnet or iron.
Furthermore, the first adsorption blocks are linear; a plurality of linear adsorption blocks are arranged in parallel.
The beneficial effect of adopting the further scheme is that: the linear adsorption blocks are arranged in parallel to ensure uniform scribing.
Further, the second adsorption part comprises a plurality of second adsorption blocks which are arranged at intervals and are arranged corresponding to the first adsorption blocks; or the two adsorption parts comprise an adsorption block, and the adsorption blocks are respectively adsorbed with the plurality of adsorption blocks correspondingly.
The beneficial effect of adopting the further scheme is that: the second adsorption block and the first adsorption block are correspondingly arranged, so that each first adsorption block can be stably adsorbed in the culture dish; the cost can be saved by arranging the adsorption block.
Furthermore, the adsorption blocks II are connected with each other through connecting rods respectively, or the adsorption blocks II are fixed or placed in a vessel respectively.
The beneficial effect of adopting the further scheme is that: the two adsorption blocks are mutually connected or fixed on the vessel through the connecting rods, the arrangement mode of the two adsorption blocks is fixed, and manual arrangement is not needed each time.
Further, the base plate of the vessel is the same as that of the culture dish, or the vessel is circular and has an inner diameter larger than the outer diameter of the base plate of the culture dish.
The beneficial effect of adopting the further scheme is that: the placing of the culture dish base plate is facilitated, and the positions of the first adsorption part and the second adsorption part are convenient to correspond.
Further, the second adsorption block is a magnet or iron.
Furthermore, the second adsorption blocks are linear; and the linear adsorption blocks II are arranged in parallel.
The beneficial effect of adopting the further scheme is that: the second adsorption block is linear, so that the first adsorption block can be conveniently and correspondingly adsorbed.
Drawings
Fig. 1 is a schematic diagram of the explosion structure of the present invention.
In the drawings, the components represented by the respective reference numerals are listed below:
100. a first adsorption part; 101. adsorbing a first block; 102. a connecting rod; 103. a handle;
200. a chassis; 300. an upper cover;
400. a second adsorption part; 401. a second adsorption block; 402 vessels.
Detailed Description
The principles and features of the present invention are described below in conjunction with the following drawings, the examples given are only intended to illustrate the present invention and are not intended to limit the scope of the present invention.
As shown in fig. 1, the cell scratch experiment apparatus of the present embodiment includes a first adsorption part 100, a culture dish, and a second adsorption part 400, wherein the culture dish is placed on the second adsorption part 400, and the first adsorption part 100 is placed in the culture dish and closely attached to an inner side surface of a bottom wall of the culture dish by matching with the second adsorption part 400; the first adsorption part 100 comprises a plurality of first adsorption blocks 101 which are arranged at intervals.
According to the experimental device, by an adsorption method, when cells are cultured in the culture dish by matching the adsorption block I and the adsorption block II, cell deletion areas are caused, so that the uniformity and the uniformity of the scratches are ensured, and the repeatability and the accuracy of a scratch experiment are achieved.
As shown in fig. 1, the first suction blocks 101 are connected to each other by connecting rods 102, or the first suction blocks 101 are fixed to a connecting plate. The first adsorption blocks are connected with each other or fixed on the connecting plate through the connecting rods, and the first adsorption blocks are convenient to take and place at one time. The connecting rod is made of an iron rod, and the connecting plate is made of an iron plate, so that high-temperature disinfection is facilitated. The first adsorption block can be adhered to the connecting rod or the connecting plate through glue, or the first adsorption block can be connected to the connecting rod or the connecting plate through a screw.
When the connecting rods are used for connection, two adjacent first adsorption blocks can be connected through one connecting rod respectively, or all the first adsorption blocks are connected to one connecting rod.
When the connection plate is used for connection, all the first adsorption blocks can be connected to one surface of the connection plate.
As shown in fig. 1, a handle 103 is connected to the connecting rod 102 or the connecting plate in this embodiment. And a handle is connected on the connecting rod or the connecting plate, so that the first adsorption block can be conveniently taken and placed by experimenters. The handle is in a U-shaped structure, and when the connecting rod is used for connection, two ends of the handle can be respectively fixed on the connecting rod or the first adsorption block; when the connecting plate is used for connection, two ends of the handle can be respectively fixed on one surface of the connecting plate far away from the first adsorption block.
As shown in fig. 1, the first adsorption block 101 of the present embodiment is a magnet or iron. The first adsorption blocks are linear; a plurality of linear adsorption blocks are arranged in parallel. The first adsorption block is linear, and the linear adsorption blocks are arranged in parallel, so that the scribing is uniform.
As shown in fig. 1, a specific scheme of this embodiment is that the first adsorption blocks 101 are three and all in a linear shape, the three first adsorption blocks 101 are arranged in parallel and at intervals, and the distance between every two adjacent first adsorption blocks 101 is the same. The cross section of the first adsorption block 101 is square, namely the first adsorption block is of a cubic structure, and particularly, as shown in fig. 1, the width of the bottom surface of the first adsorption block is smaller than the height of the first adsorption block, so that the first adsorption block has enough height to be conveniently taken and placed, and scratches formed at the bottom of the first adsorption block in a culture dish are suitable.
As shown in fig. 1, the second adsorption part 400 of the present embodiment includes a plurality of second adsorption blocks 401 arranged at intervals and corresponding to the plurality of first adsorption blocks 101; or the second adsorption part 400 comprises an adsorption block, and the adsorption blocks are respectively adsorbed corresponding to the first adsorption blocks 101. The second adsorption block and the first adsorption block are correspondingly arranged, so that each first adsorption block can be stably adsorbed in the culture dish; the cost can be saved by arranging the adsorption block.
When the second adsorption part 400 comprises an adsorption block, the adsorption block is in a sheet shape or a strip shape, and when the adsorption block is in a sheet shape, the chassis of the culture dish is directly placed on the sheet-shaped adsorption block; when this adsorbs the piece and is the strip, directly place the chassis of culture dish on this strip adsorbs the piece, makes this strip adsorb the piece and can pass the projection of a plurality of absorption piece below, make this strip adsorb the piece can both with a plurality of adsorb piece and live.
As shown in fig. 1, the two adsorption blocks 401 are respectively connected with each other through a connecting rod, or the two adsorption blocks 401 are respectively fixed or placed in a vessel 402. The two adsorption blocks are mutually connected or fixed on the vessel through the connecting rods, the arrangement mode of the two adsorption blocks is fixed, and manual arrangement is not needed each time.
Specifically, as shown in fig. 1, the culture dish includes a bottom plate 200 and an upper cover 300, and the upper cover 300 covers the bottom plate 200. The vessel 402 of this embodiment is the same as the bottom plate 200 of the culture dish, or the vessel 402 is circular and has an inner diameter larger than the outer diameter of the bottom plate 200 of the culture dish. The placing of the culture dish base plate is facilitated, and the positions of the first adsorption part and the second adsorption part are convenient to correspond.
The utensil 402 is circular and has an inner diameter larger than the outer diameter of the culture dish chassis, and the culture dish chassis can be directly placed in the utensil and is pressed on the adsorption block II.
The height of the upper surface of the second adsorption block 401 or the adsorption block is equal to the upper surface of the vessel 402.
In a specific aspect of this embodiment, the second adsorption block 401 is a magnet or iron.
In a specific implementation manner of this embodiment, the first adsorption block 101 and the second adsorption block 401 are both magnets, and the magnetic poles of the opposite portions of the first adsorption block 101 and the second adsorption block 401 are opposite. When the culture dish with the first adsorption block 101 is placed on the vessel 402, the first adsorption block 101 and the second adsorption block 401 are mutually attracted.
As shown in fig. 1, the second adsorption blocks 401 are respectively linear; the linear adsorption blocks 401 are arranged in parallel. The second adsorption block is linear, so that the first adsorption block can be conveniently and correspondingly adsorbed.
The specific scheme of this embodiment does, two adsorption blocks are three and are linear structure, the shape size of two adsorption blocks with the shape size of adsorption block one is the same, two adsorption blocks with the corresponding setting of adsorption block.
When the cell scratch experimental device is used, a cell culture dish is placed above a circular vessel with three parallel magnets inside, three parallel inferior-quality products are placed in the cell culture dish (culture medium is contained in the culture dish) and adsorbed to the three parallel magnets, cells are inoculated in the cell culture dish for cell culture, and after the cells grow to be full, the three parallel magnets in the culture dish are taken out to form three parallel scratches of cell deletion. The experiment causes cell loss through magnet adsorption, can ensure the flatness of scratches and the uniformity of width, and achieves the repeatability and accuracy of the scratch experiment. The experimental device can be disinfected at high temperature and can be recycled; further, since the magnetic field does not affect the cell culture, for example, the human body can examine the body by nuclear magnetic resonance, the position and shape of the scratch are defined by the magnet, and the cultured cells are not affected.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", and the like, indicate the orientation or positional relationship indicated based on the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
In the present application, unless expressly stated or limited otherwise, the first feature may be directly on or directly under the second feature or indirectly via intermediate members. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.
Although embodiments of the present invention have been shown and described, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art without departing from the scope of the present invention.
Claims (10)
1. A cell scratch experimental device is characterized by comprising a first adsorption part, a culture dish and a second adsorption part, wherein the culture dish is placed on the second adsorption part, and the first adsorption part is placed in the culture dish and tightly attached to the inner side surface of the bottom wall of the culture dish through being matched with the second adsorption part; the first adsorption part comprises a plurality of first adsorption blocks which are arranged at intervals.
2. The device for cell scratch experiment according to claim 1, wherein a plurality of the absorption blocks are connected to each other by a connecting rod, or a plurality of the absorption blocks are fixed on a connecting plate.
3. The device for cell scratch test according to claim 2, wherein a handle is connected to the connecting rod or the connecting plate.
4. The device for performing cytochal assay according to any one of claims 1 to 3, wherein the first adsorption block is a magnet or iron.
5. The device for performing cytochal assay according to any of claims 1 to 3, wherein a plurality of said first adsorption blocks are linear; a plurality of linear adsorption blocks are arranged in parallel.
6. The cell scratch experiment device according to any one of claims 1 to 3, wherein the second adsorption part comprises a plurality of second adsorption blocks arranged at intervals and arranged corresponding to the first adsorption blocks; or the two adsorption parts comprise an adsorption block, and the adsorption blocks are respectively adsorbed with the plurality of adsorption blocks correspondingly.
7. The device for cell scratch experiment according to claim 6, wherein a plurality of the second absorption blocks are connected to each other by connecting rods, or a plurality of the second absorption blocks are fixed or placed in a vessel.
8. The device according to claim 7, wherein the vessel is the same as the bottom plate of the culture dish, or the vessel is circular and has an inner diameter larger than an outer diameter of the bottom plate of the culture dish.
9. The device for cell scratch experiment according to claim 6, wherein the second adsorption block is a magnet or iron.
10. The device for testing cell scratch according to claim 6, wherein the second absorption blocks are respectively linear; and the linear adsorption blocks II are arranged in parallel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920745455.4U CN210103939U (en) | 2019-05-22 | 2019-05-22 | Cell mar experimental apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920745455.4U CN210103939U (en) | 2019-05-22 | 2019-05-22 | Cell mar experimental apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210103939U true CN210103939U (en) | 2020-02-21 |
Family
ID=69561000
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920745455.4U Expired - Fee Related CN210103939U (en) | 2019-05-22 | 2019-05-22 | Cell mar experimental apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210103939U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113295611A (en) * | 2021-05-22 | 2021-08-24 | 西安电子科技大学芜湖研究院 | Stay quick-witted pipe surface mar inspection device based on degree of depth learning |
-
2019
- 2019-05-22 CN CN201920745455.4U patent/CN210103939U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113295611A (en) * | 2021-05-22 | 2021-08-24 | 西安电子科技大学芜湖研究院 | Stay quick-witted pipe surface mar inspection device based on degree of depth learning |
| CN113295611B (en) * | 2021-05-22 | 2023-08-04 | 西安电子科技大学芜湖研究院 | Leave quick-witted pipe surface scratch inspection device based on degree of depth study |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN202621208U (en) | Novel test tube rack | |
| CN204714819U (en) | A kind of Tissue Culture Flask of belt supporting frame | |
| CN210103939U (en) | Cell mar experimental apparatus | |
| CN206872853U (en) | Biological cell culture apparatus | |
| CN211688982U (en) | Multifunctional biochemical incubator | |
| CN201459104U (en) | Micro-porous plate fixing device | |
| CN210853500U (en) | Sample storage box for food safety inspection | |
| CN216155880U (en) | Stem cell constant temperature culture equipment | |
| CN208007592U (en) | A kind of genetic test reagent storage case | |
| CN212025306U (en) | Culture dish case | |
| US10690574B2 (en) | Chilling apparatus | |
| CN208140716U (en) | A kind of novel agent box with paramagnetic particle method detection human IgG antibody | |
| CN203587374U (en) | Homogenization bag sample frame | |
| CN210560492U (en) | Stem cell layered co-culture device | |
| CN209039507U (en) | A kind of DC electric field tampering devic of cultured cell in vitro | |
| CN216547451U (en) | Culture apparatus for biotechnology experiments | |
| CN111269801A (en) | Culture dish case | |
| CN219670528U (en) | Cell constant temperature incubator | |
| CN104403945B (en) | The structure of cell culture board clamp | |
| CN213966711U (en) | Be used for biological laboratory culture dish rack | |
| CN215234329U (en) | Standard low-temperature constant-temperature tank with protection mechanism | |
| CN218259406U (en) | Agricultural and livestock product inspection device | |
| CN210556375U (en) | Clinical examination slide glass case | |
| CN215799505U (en) | Make things convenient for EP pipe support of embryo biopsy operation | |
| CN218969245U (en) | Carbon dioxide incubator |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200221 Termination date: 20210522 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |