CN209778891U - Novel mesenchymal stem cell culture container - Google Patents
Novel mesenchymal stem cell culture container Download PDFInfo
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- CN209778891U CN209778891U CN201822257096.6U CN201822257096U CN209778891U CN 209778891 U CN209778891 U CN 209778891U CN 201822257096 U CN201822257096 U CN 201822257096U CN 209778891 U CN209778891 U CN 209778891U
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- culture dish
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- stem cells
- mesenchymal stem
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Abstract
The utility model relates to a novel mesenchymal stem cell culture container, which comprises a plurality of culture dishes, wherein the plurality of culture dishes are sequentially stacked from top to bottom; the upper end of the culture dish at the top layer in the plurality of culture dishes is provided with a cover body, and the cover body covers the culture dish; each culture dish is of a cylindrical structure, and the lower part of each culture dish is of a sealing structure; and each culture dish is internally provided with a layer of albumin coating layer on the side wall and the bottom, and the albumin coating layers are adsorbed on the side wall or the bottom in the culture dish. Compared with the prior art, the utility model realizes the simultaneous culture of stem cells with different potentials in the same culture container; the propagation efficiency of stem cells can be improved; is beneficial to the development of stem cells, can provide nutrition for the stem cells and accelerate the propagation speed of the stem cells.
Description
Technical Field
The utility model relates to a stem cell culture technical field, in particular to novel mesenchymal stem cell culture container.
Background
Stem cells are a type of pluripotent cells that have the ability to self-replicate. Under certain conditions, it can differentiate into multiple functional cells, and according to the development stage of stem cell, it can be divided into embryonic stem cell and adult stem cell. The stem cells can be divided into three categories according to their developmental potential: totipotent stem cells, pluripotent stem cells, unipotent stem cells. The stem cell is an insufficiently differentiated and immature cell, has potential functions of regenerating various tissues, organs and human bodies, and is called as a universal cell in the medical field; therefore, stem cell culture is particularly important, but various problems of the containers for stem cell culture in the prior art, such as the inability to culture stem cells with different potentials according to different requirements, slow propagation speed and easy adherent growth of the stem cells.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a novel mesenchymal stem cell culture container, the technical problem that will solve is: stem cells with different potentials cannot be cultured according to different requirements, the propagation speed is slow, and the stem cells are easy to grow adherent.
The utility model provides an above-mentioned technical problem's technical scheme as follows: a novel mesenchymal stem cell culture container comprises a plurality of culture dishes, wherein the plurality of culture dishes are sequentially stacked from top to bottom; the upper end of the culture dish at the top layer in the plurality of culture dishes is provided with a cover body, and the cover body covers the culture dish; each culture dish is of a cylindrical structure, and the lower part of each culture dish is of a sealing structure;
And each culture dish is internally provided with a layer of albumin coating layer on the side wall and the bottom, and the albumin coating layers are adsorbed on the side wall or the bottom in the culture dish.
On the basis of the technical scheme, the utility model discloses can also do following improvement.
Further, the bottom of the inner side of each culture dish is of a wavy structure, and the height difference between the lowest part and the highest part of the wavy structure is 0.1-1 mm.
Furthermore, the upper end of the annular side wall of each culture dish is provided with an annular groove body arranged along the annular side wall; every the lower extreme of the annular lateral wall of culture dish all is provided with the annular convex body of arranging along this annular lateral wall, every annular convex body on the culture dish corresponds the annular groove internal of its lower extreme culture dish of detachable embedding.
Further, the albumin coating layer is a human serum albumin coating layer.
Further, each of the culture dishes is made of a high molecular polymer material.
further, the high molecular polymer material is polystyrene.
The utility model has the advantages that: the plurality of culture dishes can respectively culture stem cells with different potentials, so that the stem cells with different potentials can be simultaneously cultured by the same culture container; the propagation efficiency of stem cells can be improved;
the albumin coating layer can reduce the adsorption of the stem cells on the inner wall and the bottom of the culture dish, is favorable for the development of the stem cells, can provide nutrition for the stem cells and accelerates the propagation speed of the stem cells.
Drawings
Fig. 1 is a front view of a novel mesenchymal stem cell culture container of the present invention;
FIG. 2 is a schematic structural view of the culture dish of the present invention;
fig. 3 is a schematic view of the top view structure of the culture dish of the present invention.
In the drawings, the components represented by the respective reference numerals are listed below:
1. the culture dish comprises a culture dish body, 2 a cover body, 3 an albumin coating layer, 4 a wavy structure, 5 an annular groove body, 6 and an annular convex body.
Detailed Description
the principles and features of the present invention are described below in conjunction with the following drawings, the examples given are only intended to illustrate the present invention and are not intended to limit the scope of the present invention.
As shown in fig. 1 to 3, a novel mesenchymal stem cell culture container comprises a plurality of culture dishes 1, wherein the plurality of culture dishes 1 are sequentially stacked from top to bottom; the upper end of the culture dish 1 at the top layer in the plurality of culture dishes 1 is provided with a cover body 2, and the cover body 2 covers the culture dish 1; each culture dish 1 is of a cylindrical structure, and the lower part of each culture dish 1 is of a sealing structure;
An albumin coating layer 3 is arranged on the side wall and the bottom in each culture dish 1, and the albumin coating layer 3 is adsorbed on the side wall or the bottom in the culture dish 1.
The plurality of culture dishes can respectively culture stem cells with different potentials, so that the stem cells with different potentials can be simultaneously cultured by the same culture container; the propagation efficiency of stem cells can be improved;
The albumin coating layer 3 can reduce the adsorption of the stem cells on the inner wall and the bottom of the culture dish, is beneficial to the development of the stem cells, can provide nutrition for the stem cells and accelerates the propagation speed of the stem cells.
In the above embodiment, the bottom of the inner side of each culture dish 1 is a wave-shaped structure 4, and the height difference between the lowest part and the highest part of the wave-shaped structure 4 is 0.1-1 mm.
The wavy structure 4 can enable the stem cells at the bottom of the culture dish 1 to gather, accelerate the further development of the stem cells and accelerate the propagation of the stem cells.
In the above embodiment, the upper end of the annular side wall of each culture dish 1 is provided with an annular groove body 5 arranged along the annular side wall; the lower end of the annular side wall of each culture dish 1 is provided with an annular convex body 6 arranged along the annular side wall, and the annular convex body 6 on each culture dish 1 is correspondingly detachably embedded into the annular groove body 5 of the culture dish 1 at the lower end of the culture dish.
Every annular convex body 6 on the culture dish 1 corresponds in the annular cell body 5 of its lower extreme culture dish 1 of detachable embedding for a plurality of culture dishes 1 can stably range upon range of, make the stem cell in the culture dish 1 stably develop.
in the above embodiment, the albumin coating layer 3 is a human blood albumin coating layer 3.
In the above embodiment, each of the culture dishes 1 is made of a high molecular polymer material.
in the above embodiments, the high molecular polymer material is polystyrene.
The above description is only for the preferred embodiment of the present invention, and is not intended to limit the present invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included within the protection scope of the present invention.
Claims (6)
1. A novel mesenchymal stem cell culture container is characterized by comprising a plurality of culture dishes (1), wherein the plurality of culture dishes (1) are sequentially stacked from top to bottom; the culture dish (1) at the top layer in the plurality of culture dishes (1) is provided with a cover body (2) at the upper end, and the cover body (2) covers the culture dish (1); each culture dish (1) is of a cylindrical structure, and the lower part of each culture dish (1) is of a sealing structure;
An albumin coating layer (3) is arranged on the side wall and the bottom in each culture dish (1), and the albumin coating layer (3) is adsorbed on the side wall or the bottom in each culture dish (1).
2. The novel mesenchymal stem cell culture vessel according to claim 1, wherein the bottom of the inner side of each culture dish (1) is a wave-shaped structure (4), and the height difference between the lowest part and the highest part of the wave-shaped structure (4) is 0.1-1 mm.
3. the novel mesenchymal stem cell culture container according to claim 1, wherein the upper end of the annular side wall of each culture dish (1) is provided with an annular groove body (5) arranged along the annular side wall; every the lower extreme of the annular lateral wall of culture dish (1) all is provided with along annular convex body (6) that this annular lateral wall arranged, every annular convex body (6) on culture dish (1) correspond in the annular cell body (5) of its lower extreme culture dish (1) of detachable embedding.
4. A novel mesenchymal stem cell culture vessel according to claim 1, wherein the albumin coating (3) is a human blood albumin coating (3).
5. A novel mesenchymal stem cell culture vessel according to claim 1, wherein each said culture dish (1) is made of high molecular polymer material.
6. The novel mesenchymal stem cell culture container according to claim 5, wherein the high molecular polymer material is polystyrene.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822257096.6U CN209778891U (en) | 2018-12-30 | 2018-12-30 | Novel mesenchymal stem cell culture container |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822257096.6U CN209778891U (en) | 2018-12-30 | 2018-12-30 | Novel mesenchymal stem cell culture container |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209778891U true CN209778891U (en) | 2019-12-13 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201822257096.6U Active CN209778891U (en) | 2018-12-30 | 2018-12-30 | Novel mesenchymal stem cell culture container |
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| CN (1) | CN209778891U (en) |
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2018
- 2018-12-30 CN CN201822257096.6U patent/CN209778891U/en active Active
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