CN214400565U - Culture dish for non-contact cell co-culture - Google Patents
Culture dish for non-contact cell co-culture Download PDFInfo
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- CN214400565U CN214400565U CN202120140442.1U CN202120140442U CN214400565U CN 214400565 U CN214400565 U CN 214400565U CN 202120140442 U CN202120140442 U CN 202120140442U CN 214400565 U CN214400565 U CN 214400565U
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- 238000003501 co-culture Methods 0.000 title claims abstract description 17
- 238000004113 cell culture Methods 0.000 claims abstract description 88
- 238000004062 sedimentation Methods 0.000 claims abstract description 21
- 239000012528 membrane Substances 0.000 claims abstract description 10
- 239000001963 growth medium Substances 0.000 abstract description 19
- 238000009630 liquid culture Methods 0.000 abstract description 19
- 238000012258 culturing Methods 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 97
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- 108010019160 Pancreatin Proteins 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
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- 238000005034 decoration Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
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- 239000002245 particle Substances 0.000 description 2
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- 238000012360 testing method Methods 0.000 description 2
- 210000003771 C cell Anatomy 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
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- 238000012832 cell culture technique Methods 0.000 description 1
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Images
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a non-contact cell co-culture dish, which comprises a dish body and a dish cover, wherein the dish body at least comprises two cell culture tanks, a cell sedimentation tank is arranged between every two cell culture tanks, and a single cell culture tank is separated from the cell sedimentation tank by a baffle plate, so that multiple cells can be separately cultured in the same liquid culture medium system; when the baffle plate is a semi-permeable membrane, the culture dish can also be suitable for culturing semi-suspended or suspended cells.
Description
Technical Field
The utility model relates to a cell biology experiment technical field especially relates to a culture dish for non-contact type cell coculture.
Background
A technique of culturing cells taken out of the body in vitro in a manner simulating the physiological environment in the body and allowing them to survive and grow is called cell culture. Cell culture techniques are important and commonly used in cell biology research methods, and a large number of cells can be obtained through cell culture, and signal transduction, anabolism, growth and proliferation of cells and the like of the cells can be researched. The in vitro cell culture method includes adherent culture, semi-adherent culture, suspension culture, etc., and the adherent culture is the most common. When the cells are cultured in the adherent mode, the liquid culture medium not only can provide nutrient components for the growth and proliferation of the cells, but also can provide cytokines and the like necessary for maintaining the normal physiological functions of the cells, provides proper temperature, osmotic pressure, pH value and oxygen and carbon dioxide concentrations for the growth of the cells, and can be said to directly form the internal environment for the growth of the cells.
In the late stage of the 80's of the 20 th century, in order to establish a culture system more similar to the in vivo environment, the in vitro environment and the in vivo environment are matched as much as possible, so that the cells can communicate information with each other and support each other for growth and proliferation, and people develop a cell co-culture technology on the basis of the cell culture technology. The cell co-culture technique is to co-culture 2 or more cells in the same environment, and is widely applied to modern cell research due to the advantage of better reflecting the in vivo environment.
Cell co-culture systems are mainly established by two methods: 1. a direct co-culture system, namely 2 or more than 2 cells are simultaneously or respectively inoculated in the same hole, and different types of cells are in direct contact with each other, and different cells grow in the same cell container, so that various cells cannot be separated for the next research, and the application of the cells is limited; 2. an indirect co-culture system, i.e. 2 or more than 2 cells are respectively inoculated on different carriers, then the two carriers are placed in the same culture environment (liquid culture medium), so that different types of cells share the same culture system without direct contact, but such a culture system has requirements on the spacing between different carriers, if the operation is slightly shaken, the cells on one carrier can move to the other carrier, and the pollution and the mixing of different cells are caused; in addition, the culture system is only suitable for adherent cells and is not suitable for the culture of suspension and semi-suspension cells.
In addition, the existing cell co-culture system has a small maximum number of cells that can be cultured in one culture, and it is difficult to collect enough cells for experiments requiring a large number of cells such as flow cytometry, a model for animal transplantation of cells, and the like.
Disclosure of Invention
For the not enough of prior art above avoiding, the utility model provides a non-contact cell coculture's culture dish to the problem that the cell mixes (the difficult separation of mutual contact) in the solution coculture system.
The utility model provides a technical scheme as follows:
a culture dish for non-contact cell co-culture comprises a dish body and a dish cover, wherein the dish body comprises at least two cell culture tanks, a cell sedimentation tank is arranged between every two cell culture tanks, and a baffle is arranged between a single cell culture tank and the cell sedimentation tank; the height of the baffle is less than that of the dish body.
Furthermore, when the dish body comprises more than two cell culture tanks, the cell sedimentation tanks between every two cell culture tanks are communicated with each other to form a whole.
Furthermore, the bottom areas of the individual cell culture tanks are different in size.
Furthermore, the baffle is a detachable baffle.
Still further, the baffle is made of a semi-permeable membrane.
Compared with the prior art, the utility model has the advantages of it is following:
the culture dish for non-contact cell co-culture provided by the utility model separates different cells by arranging the cell sedimentation tank, so that the free cells are precipitated in the cell sedimentation tank and can not be dissociated in other cell culture tanks, thereby ensuring that a plurality of cells are separately cultured under the same liquid culture medium system; in addition, because the cell sedimentation tank has an effect of hindering free cells, when the baffle plate adopts a semi-permeable membrane, the semi-permeable membrane can be suitable for culturing semi-suspended or suspended cells.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the description of the embodiments or the prior art are briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic structural view of a culture dish according to embodiment 1 of the present invention;
FIG. 2 is a longitudinal sectional view of a culture dish according to example 1 of the present invention;
FIG. 3 is a schematic structural view of a culture dish according to embodiment 2 of the present invention;
FIG. 4 is a plan view of a culture dish according to example 2 of the present invention;
in the figure: 1-dish body; 2-capsule cover; 11-a first cell culture tank; 12-a second cell culture tank; 13-cell sedimentation tank; 14-a baffle; 15-third cell culture tank.
Detailed Description
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following description will be made with reference to the accompanying drawings. It is obvious that the drawings in the following description are only some examples of the invention, and that for a person skilled in the art, other drawings and embodiments can be derived from them without inventive effort.
For the sake of simplicity, the drawings only schematically show the parts relevant to the present invention, and they do not represent the actual structure as a product. In addition, in order to make the drawings concise and understandable, components having the same structure or function in some of the drawings are only schematically illustrated or only labeled. In this document, "one" means not only "only one" but also a case of "more than one".
Example 1
The culture dish for non-contact cell co-culture shown in fig. 1 and 2 comprises a dish body 1 and a dish cover 2, wherein the dish body 1 comprises a first cell culture tank 11 and a second cell culture tank 12, a cell sedimentation tank 13 is arranged between the first cell culture tank 11 and the second cell culture tank 12, and the first cell culture tank 11 and the second cell culture tank 12 are both separated from the cell sedimentation tank 13 by a baffle 14.
In this embodiment, the height of the baffle 14 is smaller than the height of the dish body 1, and when the liquid surface of the liquid culture medium in the dish body 1 is lower than the height of the baffle 14, the first cell culture tank 11 and the second cell culture tank 12 respectively form an independent culture environment; when the liquid medium level is higher than the baffle 14, the liquid medium can flow to the contralateral cell culture tank through the cell sedimentation tank 13.
In this example, two cells were seeded. A, B adherent cells cultured in vitro to logarithmic growth phase are respectively subjected to trypsinization and wall detachment in a sterile operating table, then the digestion is stopped by a liquid culture medium containing Fetal Bovine Serum (FBS), and the cells are blown off to form cell suspensions. After cell counting, respectively taking A, B cell suspensions with proper volumes by using a pipette, respectively inoculating the cell suspensions into a first cell culture tank 11 and a second cell culture tank 12 of a culture dish for non-contact cell co-culture, then adding a liquid culture medium to a position below the height of a baffle 14, so that the first cell culture tank 11 and the second cell culture tank 12 respectively form an independent culture system, and placing the culture dish in a cell culture box. And (3) determining the cell adherence condition by observing through a microscope at regular time according to the adherence time of different cell lines, continuously and slowly adding a liquid culture medium after the cells adhere to the wall until the liquid level is higher than the height of the baffle plate 14, and then continuously placing the culture dish in a cell culture box. After a period of time, the cell culture dish is removed from the incubator and placed on a sterile operating table. Liquid medium is slowly aspirated from the cell sedimentation tank 13 by a pipette until the liquid level is level with the height of the baffle 14, and aspiration is continued until all liquid in the cell sedimentation tank 13 is completely aspirated. The culture dish is tilted, and the liquid medium in the first cell culture vessel 11 and the second cell culture vessel 12 is completely aspirated by a pipette, respectively. A small amount of 2 to 5% pancreatin solution is added to the first cell culture tank 11 and the second cell culture tank 12, respectively, and the liquid level cannot be higher than the baffle plate 14. After digesting the cells to remove the wall, the digestion is terminated, the dish is tilted, and the cell suspension in the first cell culture vessel 11 and the second cell culture vessel 12 is taken up by a pipette. The harvested cell suspension may be passaged or used for other cell biology experiments.
In one embodiment, the bottom areas of the first cell culture tank 11 and the second cell culture tank 12 are different in size to adapt to the growth of cells with different growth speeds, and the cells with the higher growth speed can be inoculated into the larger culture tank to achieve the synchronization of the growth stages of the two cells with different growth speeds; on the other hand, the comparative test of a plurality of different numbers of cells can also be realized.
In one embodiment, the baffle 14 is a detachable baffle, and the volume of the first cell culture tank or the second cell culture tank is adjusted according to the height of the baffle, so that the volume of the liquid culture medium or the cells can be controlled according to the amount of the cells to be cultured in the experiment.
In one embodiment, the baffle 14 is made of a semi-permeable membrane, and the particles that can penetrate the baffle can be adjusted according to the experimental content (the diameter of the molecules that can penetrate the baffle can be limited by controlling the pore size of the semi-permeable membrane), for example, the molecules that can pass through the liquid culture medium can be selected, but the semi-permeable membrane that can not pass through the cell can be selected, so that the liquid culture medium can freely pass through the baffle when the cell is cultured, and the purpose that the cell culture environment in different cell culture tanks is the same is achieved.
Example 2
The culture dish for non-contact cell co-culture as shown in fig. 3 and 4 comprises a dish body 1 and a dish cover 2, wherein the dish body 1 comprises a first cell culture tank 11, a second cell culture tank 12 and a third cell culture tank 15, a cell sedimentation tank 13 is arranged between the first cell culture tank 11, the second cell culture tank 12 and the third cell culture tank 15, and the first cell culture tank 11, the second cell culture tank 12 and the third cell culture tank 15 are separated from the cell sedimentation tank 13 by a baffle 14.
In this embodiment, the height of the baffle 14 is smaller than the height of the dish body 1, and when the liquid surface of the liquid culture medium in the dish body 1 is lower than the height of the baffle 14, the first cell culture tank 11, the second cell culture tank 12 and the third cell culture tank 15 respectively form an independent culture environment; when the liquid medium level is higher than the baffle plate 14, the liquid medium can flow to other cell culture tanks through the cell precipitation tank 13.
In this example, three cells were seeded. A, B, C adherent cells cultured in vitro to logarithmic phase are subjected to trypsinization and desquamation respectively in a sterile operating platform, then the digestion is stopped by a liquid culture medium containing Fetal Bovine Serum (FBS), and the cells are blown off to form cell suspension. After cell counting, respectively taking A, B, C cell suspensions with proper volumes by using a pipette, respectively inoculating the three cell suspensions into a first cell culture tank 11, a second cell culture tank 12 and a third cell culture tank 15 of a culture dish for non-contact cell co-culture, then adding a liquid culture medium to a position below the height of a baffle plate 14, so that the first cell culture tank 11, the second cell culture tank 12 and the third cell culture tank 15 respectively form an independent culture system, and placing the culture dish in a cell culture box. And (3) determining the cell adherence condition by observing through a microscope at regular time according to the adherence time of different cell lines, continuously and slowly adding a liquid culture medium after the cells adhere to the wall until the liquid level is higher than the height of the baffle plate 14, and then continuously placing the culture dish in a cell culture box. After a period of time, the cell culture dish is removed from the incubator and placed on a sterile operating table. Liquid medium is slowly aspirated from the cell sedimentation tank 13 by a pipette until the liquid level is level with the height of the baffle 14, and aspiration is continued until all liquid in the cell sedimentation tank 13 is completely aspirated. The culture dish is tilted, and the liquid culture medium in the first cell culture vessel 11, the second cell culture vessel 12 and the third cell culture vessel 15 is completely sucked up by a pipette. A small amount of 2 to 5 percent pancreatin solution is added into the first cell culture tank 11, the second cell culture tank 12 and the third cell culture tank 15 respectively, and the liquid level can not be higher than the baffle plate 14. After digesting the cells until the cell walls are removed, the digestion is terminated, and the cell suspension in the first cell culture vessel 11, the second cell culture vessel 12 and the third cell culture vessel 15 is taken up by tilting the culture dish using a pipette. The harvested cell suspension may be passaged or used for other cell biology experiments.
In one embodiment, the bottom areas of the first cell culture tank 11, the second cell culture tank 12 and the third cell culture tank 15 are different in size to adapt to the growth of cells with different growth speeds, and the cells with higher growth speed can be inoculated into the larger culture tank to achieve the synchronization of the growth stages of a plurality of cells with different growth speeds; on the other hand, the comparative test of a plurality of different numbers of cells can also be realized.
In one embodiment, the baffle 14 is a detachable baffle, and the volume of the first cell culture tank, the second cell culture tank or the third culture tank is adjusted according to the height of the baffle, so that the volume of the liquid culture medium or the cells can be controlled according to the amount of the cells to be cultured in the experiment.
In one embodiment, the baffle 14 is made of a semi-permeable membrane, and the particles that can pass through the baffle can be adjusted according to the experimental content, for example, the semi-permeable membrane that can pass through the liquid culture medium but not pass through the cells can be selected, so that the liquid culture medium can freely pass through the baffle when the cells are cultured, and the purpose of the same cell culture environment in different cell culture tanks can be achieved.
It should be noted that the above embodiments can be freely combined as necessary. The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (5)
1. A culture dish for non-contact cell co-culture comprises a dish body and a dish cover, and is characterized in that the dish body comprises at least two cell culture tanks, a cell sedimentation tank is arranged between every two cell culture tanks, and a baffle is arranged between a single cell culture tank and the cell sedimentation tank; the height of the baffle is less than that of the dish body.
2. The culture dish according to claim 1, wherein when the dish body comprises more than two cell culture tanks, the cell sedimentation tanks between each two cell culture tanks are communicated with each other to form a whole.
3. The culture dish of claim 1, wherein the bottom areas of the individual cell culture wells are sized differently.
4. The culture dish of claim 1, wherein the baffle is a removable baffle.
5. A culture dish according to claim 1, characterized in that the baffle is made of a semi-permeable membrane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120140442.1U CN214400565U (en) | 2021-01-19 | 2021-01-19 | Culture dish for non-contact cell co-culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120140442.1U CN214400565U (en) | 2021-01-19 | 2021-01-19 | Culture dish for non-contact cell co-culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214400565U true CN214400565U (en) | 2021-10-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120140442.1U Expired - Fee Related CN214400565U (en) | 2021-01-19 | 2021-01-19 | Culture dish for non-contact cell co-culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214400565U (en) |
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2021
- 2021-01-19 CN CN202120140442.1U patent/CN214400565U/en not_active Expired - Fee Related
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Granted publication date: 20211015 |
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