CN201665682U - Polyclonal co-culture ware in vitro - Google Patents
Polyclonal co-culture ware in vitro Download PDFInfo
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- CN201665682U CN201665682U CN 201020163849 CN201020163849U CN201665682U CN 201665682 U CN201665682 U CN 201665682U CN 201020163849 CN201020163849 CN 201020163849 CN 201020163849 U CN201020163849 U CN 201020163849U CN 201665682 U CN201665682 U CN 201665682U
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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Abstract
The utility model relates to a polyclonal co-culture ware in vitro, comprising a housing (1), a film (2) and a separator (3). The utility model is characterized in that the film (2) made from polycarbonate membrane is arranged in the housing (1) which is divided into a plurality of cavities by the film (2), the detachable separator (3) is arranged next to the film (2). The utility model is advantageous in that the structure is simple; the complicated environment for cells in vivo can be simulated to the largest extent; a plurality of cells which may be mutually affected can be cultured in the same environment without contacting with each other, but the cytokine secretion can interact with each other and the positive/negative feedback influence can be completed.
Description
(1) technical field
The utility model relates to a kind of in-vitro multicellular line coculture dish, belong to that biological medicine is tested or bio-pharmaceuticals in the cell culture apparatus field.
(2) background technology
Cell cultures is an important component part of medical scientific experiment in vitro.Conventional cell cultures is exactly to implant in the culture dish (bottle or plate) with a kind of cell, the nutritive ingredient that needs by the adding cell makes cell in growth in vitro, obtain a large amount of cells or its meta-bolites, use the corresponding techniques means that it is detected then, the gained result is analyzed acquire a kind of physiology or pathological phenomenon.Traditional Tissue Culture Dish can cultured cell in vitro, checks a kind of adjusting factor or result, and in the body complicated iuntercellular to regulate mutually be that multifactor or sequential influence can not be simulated external all sidedly.This is because traditional cell in vitro is cultivated used Tissue Culture Dish, can only be used as a kind of cell cultures, single cell system is isolated to independently breeds in the environment, different fully with the environment that connects each other by the recycle system and endocrine system between each histocyte in the body.In fact, a kind of growth of cell often is subjected to the adjusting of multiple condition, is not isolated the existence, and their target cell growth is usually followed and affected to the hormone of other emiocytosises or adjusting molecule in the body.This just makes traditional cell in vitro cultivate should use the limitation that seems, even the vitro culture experiment is bigger with experimentation on animals gained result error.
Development along with modern molecular biology and medical science, people begin to add investigator oneself by the composition of changing substratum or later stage in culture environment and wish that biotic factor or the molecule observed influence cultured cells, make it near many cells polymolecular coexisted environment in the body, but for environment complicated and changeable in the body, still have deviation.For example, the hormone α effect lung B cell of liver A emiocytosis in the body can make lung B cell produce hormone β; People are by extracting the hormone α of liver A cell, hormone α is joined in the vitro culture B enchylema, observe hormone α produces hormone β to the B cell influence, so very loaded down with trivial details, can't observe the feedback inhibition hormone α excretory phenomenon of the secreted hormone β of B cell under the normal physiological state to the A cell.
Just because of the limitation of existing extracorporeal culturing method, need seek new culture system near internal milieu.In suspension cell culture, add the clone of multiple different sources, can expand the environment of the suffered adjusting of suspension cell biglyyer, thereby can access more believable result; It also is a kind of co-culture method of cell that the cell that utilizes the transwell cell to carry out is migrated.Yet suspension cell is cultivated to migrate with cell altogether all its defective, the one, and substratum can't be unified, and the 2nd, very inconvenient in the observation of cell growth.
(3) summary of the invention
The purpose of this utility model is to overcome above-mentioned deficiency, a kind of in-vitro multicellular line coculture dish is provided, it is simple in structure, can may in same environment, cultivate by interactional cell multiple, iuntercellular does not contact mutually, but its excretory cytokine can interact, thereby in-vitro simulated body physiological environment is finished the influence of iuntercellular positive-negative feedback.
The purpose of this utility model is achieved in that a kind of in-vitro multicellular line coculture dish, it comprises housing, it is characterized in that: it also comprises barrier film and dividing plate, described barrier film is arranged in the housing, housing is divided into several chambers, described barrier film is other to be provided with dividing plate, and described dividing plate is detachable dividing plate; Described barrier film material is a polycarbonate membrane.
The beneficial effect of the utility model in-vitro multicellular line coculture dish is:
The utility model in-vitro multicellular line coculture dish, it is simple in structure, complicated cells survival environment in the analogue body to greatest extent, by the utility model vitro culture ware, may in same environment, cultivate by interactional cell multiple, iuntercellular does not contact mutually, but its excretory cytokine can interact, thereby in-vitro simulated body physiological environment, just finish iuntercellular, the reverse feedback influence need not to design loaded down with trivial details experimental procedure again, does not promptly need its excretory cytokine of extracting from a flask culture liquid, be injected into another bottle, in a culture dish, finish the cell internal secretion or the paracrine experiment of a complexity.
(4) description of drawings
Fig. 1 is the utility model in-vitro multicellular line coculture dish embodiment 1 structural representation.
Fig. 2 is the utility model embodiment 2 structural representations.
Among the figure: housing 1, barrier film 2, dividing plate 3.
(5) embodiment
Embodiment 1:
Referring to Fig. 1, a kind of in-vitro multicellular line coculture dish that the utility model relates to is made up of housing 1, barrier film 2 and dividing plate 3, described barrier film 2 is arranged in the housing 1, housing 1 is divided into two chambers, and described barrier film 2 sides are provided with dividing plate 3, and described dividing plate 3 is detachable dividing plate;
Described barrier film 2 materials are polycarbonate membrane;
Described dividing plate 3 materials are plastics, and are identical with housing 1 material.
Embodiment 2:
The difference of present embodiment and embodiment 1 only is that the longitudinal and transverse setting in housing of described barrier film 2 is divided into four chambers with housing 1.
The utility model in-vitro multicellular line coculture dish can produce bottle or plate different size specifications, different compartments.
Principle of work:
With one or morely can only allow the emiocytosis factor or molecule freely pass through, the intransitable polycarbonate barrier film of cell with two or more not the cell of system of the same race separately cultivate, the other device of polycarbonate barrier film has plastic septum, take out out one or more dividing plates according to the experiment needs, " group cultivates " like this can be simulated the various kinds of cell mechanism of environmental facies intermodulation control influence in vivo, for the vitro culture experiment provides perfect system more, especially provide better research tool by informational molecule interlocking effects such as secreting hormone regulation and control for various kinds of cell.
Claims (2)
1. in-vitro multicellular line coculture dish, it comprises housing (1), it is characterized in that: it also comprises barrier film (2) and dividing plate (3), described barrier film (2) is arranged in the housing (1), housing (1) is divided into several chambers, described barrier film (2) is other to be provided with dividing plate (3), and described dividing plate (3) is detachable dividing plate; Described barrier film (2) material is a polycarbonate membrane.
2. a kind of in-vitro multicellular line coculture dish according to claim 1 is characterized in that: described dividing plate (3) material is plastics.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201020163849 CN201665682U (en) | 2010-04-20 | 2010-04-20 | Polyclonal co-culture ware in vitro |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201020163849 CN201665682U (en) | 2010-04-20 | 2010-04-20 | Polyclonal co-culture ware in vitro |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201665682U true CN201665682U (en) | 2010-12-08 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201020163849 Expired - Fee Related CN201665682U (en) | 2010-04-20 | 2010-04-20 | Polyclonal co-culture ware in vitro |
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| CN (1) | CN201665682U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102329726A (en) * | 2011-09-01 | 2012-01-25 | 山西医科大学 | Cell culture dish |
-
2010
- 2010-04-20 CN CN 201020163849 patent/CN201665682U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102329726A (en) * | 2011-09-01 | 2012-01-25 | 山西医科大学 | Cell culture dish |
| CN102329726B (en) * | 2011-09-01 | 2016-03-02 | 山西医科大学 | Tissue Culture Dish |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20101208 Termination date: 20180420 |
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| CF01 | Termination of patent right due to non-payment of annual fee |