CN205473757U - To difficult separating culture device of cultivateing microorganism - Google Patents
To difficult separating culture device of cultivateing microorganism Download PDFInfo
- Publication number
- CN205473757U CN205473757U CN201620255953.7U CN201620255953U CN205473757U CN 205473757 U CN205473757 U CN 205473757U CN 201620255953 U CN201620255953 U CN 201620255953U CN 205473757 U CN205473757 U CN 205473757U
- Authority
- CN
- China
- Prior art keywords
- culture dish
- nutrition
- culture
- microorganism
- dish plate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 244000005700 microbiome Species 0.000 title claims abstract description 46
- 235000016709 nutrition Nutrition 0.000 claims abstract description 39
- 230000035764 nutrition Effects 0.000 claims abstract description 36
- 238000000926 separation method Methods 0.000 claims description 25
- 238000012549 training Methods 0.000 claims description 7
- 239000011159 matrix material Substances 0.000 claims 1
- 229920001817 Agar Polymers 0.000 abstract description 16
- 239000008272 agar Substances 0.000 abstract description 16
- 235000015097 nutrients Nutrition 0.000 abstract description 7
- 239000007787 solid Substances 0.000 abstract description 7
- 239000001963 growth medium Substances 0.000 abstract description 4
- 230000004888 barrier function Effects 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract description 3
- 206010028980 Neoplasm Diseases 0.000 abstract 1
- 235000015872 dietary supplement Nutrition 0.000 abstract 1
- 230000001815 facial effect Effects 0.000 abstract 1
- 238000011081 inoculation Methods 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 7
- 102000013275 Somatomedins Human genes 0.000 description 7
- 239000002609 medium Substances 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 241001453382 Nitrosomonadales Species 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a to difficult separating culture device of cultivateing microorganism. The device divide into culture dish lid, culture dish board and nutrition tray triplex. When just putting, in the nutrition tray was arranged in to the culture dish board, the culture dish lid was lived to the culture dish lid. The bottom surface sets up three screens in the nutrition tray, and culture dish board bottom sets up three draw -in groove. In when falling the culture medium, screens, culture dish board bottom and the subsides of nutrition tray inner bottom facial features are gone into to the draw -in groove, the inoculation is during the microorganism, and the screens is staggered with the draw -in groove, culture dish board bottom is unsettled arrange the nutrition tray in, nutrients is through the capillary action of solid agar by the agar little tumor in top layer in the nutrition tray. The utility model discloses to hard cultivateing the microorganism to solid -state agar guarantees as the isolation barrier that the microorganism is not contaminated, add as required or the nutritional supplements material, satisfy the microorganism and cultivate the requirement for a long time, utilize agar capillary action to provide the nutrient, guarantee that the nutrient supply is balanced, the device simple structure is used easy and simple to handlely.
Description
Technical field
This utility model belongs to field of experiment equipment, is specifically related to a kind of separation training for difficult cultivating microorganism
Support device.
Background technology
Microorganism is widely present in terrestrial ecosystem, and existence, health with the mankind are closely bound up.By dividing
From purification, it is thus achieved that microorganism is purebred, contribute to recognizing microorganism and controlling microorganism rule of life.But limit
In knowledge and technology level, the strain quantity of current separated research only takes up an area the 1% of ball microbial total quantity,
Urgently invention new separation technology and method are solved.
Difficult cultivating microorganism can be divided into two classes, a class to be somatomedin scarcity types, another kind of be for time cross elongated.
Somatomedin scarcity type difficulty cultivating microorganism refers to well-grown in habitat in position, but cannot obtain at laboratory
Obtain the microorganism of pure training.Such as, certain strain growth need to using the metabolite of other bacterial strain as somatomedin,
And people do not know maybe cannot provide this type of somatomedin, this bacterial strain is caused pure not train.For time cross elongated difficulty training
Foster microorganism refers to poor growth, for time longer microorganism.With traditional microbiological partition method (as flat board is drawn
Collimation method) when separating this quasi-microorganism, owing to microorganism is for duration, solid training before microorganism forms bacterium colony
Foster base is the most withered, causes mask work failure.Such as, the Dai Shiwei of anaerobic ammonia oxidizing bacteria 11 days, formed
Macroscopic bacterium colony, at least needs six months, and the most freshness-retained 10 days of solid agar medium.
For the problems referred to above, this utility model devises a kind of separation and Culture device for difficult cultivating microorganism,
And establish corresponding isolated culture method, by arranging nutrition pallet, utilize the barrier action of solid-state agar
And capillarity, make plan separate microorganism in nutrition pallet, nutritional solution obtains the necessary growth of growth because of
Son and nutrient substance, efficiently solve somatomedin scarcity type and Dai Shi crosses the separation of elongated difficulty cultivating microorganism
Cultivate a difficult problem.
Summary of the invention
The purpose of this utility model is to solve the problem that existing microbe separation technology exists, it is provided that a kind of for
The separation and Culture device of difficult cultivating microorganism, utilizes barrier action and the capillarity of solid-state agar, it is to avoid mesh
Mark bacterium suffers living contaminants, on-demand interpolation nutrient substance, it is ensured that object bacteria obtains nutrient for a long time, it is achieved difficult training
Support the separation and Culture of microorganism.
To achieve these goals, the concrete technical scheme that this utility model uses is as follows:
A kind of separation and Culture device for difficult cultivating microorganism, device is divided into culture dish lid, culture dish plate and
Nutrition pallet three part.When just putting, culture dish plate is placed in nutrition pallet, and culture dish lid is by culture dish plate lid
Live.Nutrition pallet inner bottom surface is uniformly arranged three screens, screens and nutrition pallet adhesion according to wall；Culture dish plate
Base plate hollow, arranges contact disc, can be affixed with nutrition pallet inner bottom surface, and culture dish plate side outer wall sets
Putting high scale line and lower graduation mark, culture dish plate outer bottom edge is uniformly arranged three draw-in grooves.
As preferably, described culture dish lid internal diameter is 85mm, interior deep 5mm, and wall thickness is 1mm；Cultivate
Ware plate internal diameter is 82mm, interior deep 10mm, and wall thickness is 1mm；Nutrition pallet internal diameter is 92mm, the most deeply
10mm, wall thickness is 1mm.
As preferably, described screens section triangle at a right angle, right angle bottom side length 7.3mm, right angle flap
Long 2.9mm；Screens thickness is 1mm；Screens is uniformly distributed in nutrition pallet inner bottom surface, angle each other
α is 120 °.
As preferably, contacting disc internal diameter bottom described culture dish plate is 74mm.
As preferably, described culture dish plate outer wall high scale line exceeds culture dish plate inner bottom surface 6.5mm, refers to
Show that culture volume is 35ml；Lower graduation mark exceeds culture dish plate inner bottom surface 4mm, and indicator medium volume is
20ml。
As preferably, described card grooved profile triangle at a right angle, right angle bottom side length 3.2mm, right angle flap
Long 1.5mm；Card well width is 1mm；Draw-in groove is uniformly distributed in culture dish plate outer bottom edge, each other
Angle beta is 120 °.
The beneficial effects of the utility model are: 1) device simple structure, use easy to operate；2) for life
Long factor scarcity type difficulty cultivating microorganism, can feed the growth of microorganism factor by providing separation source lixiviating solution；
3) for for time cross elongated difficulty cultivating microorganism, supplemented medium can be passed through, it is ensured that the nutrition of growth of microorganism
Material.
Accompanying drawing explanation
Fig. 1 is a kind of separation and Culture device profile for difficult cultivating microorganism, and screens enters draw-in groove, can fall
Enter agar culture medium；
Fig. 2 is a kind of separation and Culture device profile for difficult cultivating microorganism, and screens misplaces with draw-in groove,
For quiescent culture；
Fig. 3 is a kind of separation and Culture device top view for difficult cultivating microorganism；
Fig. 4 is a kind of separation and Culture device culture dish plate (2) front view for difficult cultivating microorganism；
Fig. 5 is a kind of separation and Culture device culture dish plate (2) top view for difficult cultivating microorganism；
In figure: culture dish lid 1, culture dish plate 2, nutrition pallet 3, screens 4, contact disc 5, high scale
Line 6, lower graduation mark 7, draw-in groove 8.
Detailed description of the invention
Below in conjunction with concrete the drawings and specific embodiments, this utility model is described further.If there is no spy
Different explanation or conflict, each preferred implementation can be in any combination.
As shown in Fig. 1,2,3,4,5, a kind of separation and Culture device for difficult cultivating microorganism.Device
It is divided into culture dish lid 1, culture dish plate 2 and nutrition pallet 3 three part.When just putting, culture dish plate 2 is placed in battalion
Supporting in pallet 3, culture dish plate 2 is covered by culture dish lid 1.Nutrition pallet 3 inner bottom surface is uniformly arranged according to wall
Three screens 4, screens 4 and nutrition pallet 3 adhesion；Culture dish plate 2 base plate hollow, arranges contact disc 5,
Can be affixed with nutrition pallet 3 inner bottom surface, culture dish plate 2 side outer wall arranges high scale line 6 and lower scale
Line 7, culture dish plate 2 outer bottom edge is uniformly arranged three draw-in grooves 8
Size and the visual practical situation of ratio of above-mentioned each device are set.In the present embodiment, described
Culture dish lid 1 internal diameter be 85mm, interior deep 5mm, wall thickness is 1mm；Culture dish plate 2 internal diameter is 82mm,
Interior deep 10mm, wall thickness is 1mm；Nutrition pallet 3 internal diameter is 92mm, a height of 10mm, and wall thickness is 1mm.
Described screens 4 section triangle at a right angle, right angle bottom side length 7.3mm, the long 2.9mm of right angle flap；Card
Position 4 thickness are 1mm；Screens 4 is uniformly distributed in nutrition pallet 3 inner bottom surface, and angle [alpha] is 120 ° each other.
Contacting disc 5 internal diameter bottom described culture dish plate 2 is 74mm.Described culture dish plate 2 outer wall high scale
Line 6 exceeds culture dish plate 2 inner bottom surface 6.5mm, and indicator medium volume is 35ml；Lower graduation mark 7 exceeds
Culture dish plate 2 inner bottom surface 4mm, indicator medium volume is 20ml.Described draw-in groove 8 section at a right angle three
Dihedral, right angle bottom side length 3.2mm, the long 1.5mm of right angle flap；Draw-in groove 8 width is 1mm；Draw-in groove 8 is equal
The even culture dish plate 2 outer bottom edge that is distributed in, angle beta is 120 ° each other.Through overtesting, above-mentioned chi
Very little and ratio can preferably complete test purpose of the present utility model.
A kind of separation and Culture device for difficult cultivating microorganism uses polystyrene to make.
Based on said apparatus, this utility model additionally provides a kind of separation and Culture side for difficult cultivating microorganism
Method, step is as follows:
Nutrition pallet 3 is just put, and is placed in nutrition pallet 3 by culture dish plate 2, makes screens 4 be inserted into draw-in groove 8,
Contact disc 5 bottom culture dish plate 2 to be affixed with nutrition pallet 3 inner bottom surface；The dissolved solid agar is cultivated
Culture dish plate 2 poured into by base, covers culture dish lid 1；After agar solidification, take away culture dish lid 1, at agar
Solid culture primary surface carries out scribing operation, covers culture dish lid 1；Gently lift culture dish plate 2, rotate and make card
Groove 8 staggers with screens 4, and now culture dish plate 2 is unsettled is placed in nutrition pallet 3；In nutrition pallet 3
Inject strain growth desired nutritional material, quiescent culture.
The final liquid level pouring the dissolved solid agar medium in culture dish plate 2 into is in lower graduation mark more than 6,
Ensure that agar layer has certain thickness, it is to avoid in incubation, sink in agar layer center；Culture dish plate 2 falls
The final liquid level entering culture medium is in high scale line less than 5, it is ensured that agar layer thickness is suitable, strengthens agar layer
Capillarity, meets the nutritional need of microorganism.
This utility model simple structure, easy to operate.The training of elongated difficulty is crossed for somatomedin scarcity type and Dai Shi
Support microorganism, can be by adding strain separating source lixiviating solution and microbiological culture media to nutrition pallet 3, for mesh
Mark bacterium supplements somatomedin and nutrient substance, cracks a separation and Culture difficult problem for difficult cultivating microorganism.
Claims (6)
1. the separation and Culture device for difficult cultivating microorganism, it is characterised in that: device is divided into culture dish
Lid (1), culture dish plate (2) and nutrition pallet (3) three part, when just putting, culture dish plate (2) is placed in
In nutrition pallet (3), culture dish plate (2) is covered by culture dish lid (1)；Nutrition pallet (3) inner bottom
Face is uniformly arranged three screens (4), screens (4) and nutrition pallet (3) adhesion according to wall；Culture dish plate (2)
Base plate hollow, arranges contact disc (5), can be affixed with nutrition pallet (3) inner bottom surface, culture dish plate (2)
Arranging high scale line (6) and lower graduation mark (7) on side outer wall, culture dish plate (2) outer bottom edge is equal
Even three draw-in grooves (8) are set.
Separation and Culture device for difficult cultivating microorganism the most according to claim 1, it is characterised in that:
Described culture dish lid (1) internal diameter is 85mm, interior deep 5mm, and wall thickness is 1mm；Culture dish plate (2)
Internal diameter is 82mm, interior deep 10mm, and wall thickness is 1mm；Nutrition pallet (3) internal diameter is 92mm, the most deeply
10mm, wall thickness is 1mm.
Separation and Culture device for difficult cultivating microorganism the most according to claim 1, it is characterised in that:
Described screens (4) section triangle at a right angle, right angle bottom side length 7.3mm, the long 2.9mm of right angle flap；
Screens (4) thickness is 1mm；Screens (4) is uniformly distributed in nutrition pallet (3) inner bottom surface, each other
Angle [alpha] is 120 °.
Separation and Culture device for difficult cultivating microorganism the most according to claim 1, it is characterised in that:
Described culture dish plate (2) bottom contact disc (5) internal diameter is 74mm.
Separation and Culture device for difficult cultivating microorganism the most according to claim 1, it is characterised in that:
Described culture dish plate (2) outer wall high scale line (6) exceeds culture dish plate (2) inner bottom surface 6.5mm, refers to
Show that culture volume is 35ml；Lower graduation mark (7) exceeds culture dish plate (2) inner bottom surface 4mm, instruction training
Foster matrix amasss as 20ml.
Separation and Culture device for difficult cultivating microorganism the most according to claim 1, it is characterised in that:
Described draw-in groove (8) section triangle at a right angle, right angle bottom side length 3.2mm, the long 1.5mm of right angle flap；
Draw-in groove (8) width is 1mm；Draw-in groove (8) is uniformly distributed in culture dish plate (2) outer bottom edge, mutually
Between angle beta be 120 °.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620255953.7U CN205473757U (en) | 2016-03-30 | 2016-03-30 | To difficult separating culture device of cultivateing microorganism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620255953.7U CN205473757U (en) | 2016-03-30 | 2016-03-30 | To difficult separating culture device of cultivateing microorganism |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205473757U true CN205473757U (en) | 2016-08-17 |
Family
ID=56647774
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620255953.7U Active CN205473757U (en) | 2016-03-30 | 2016-03-30 | To difficult separating culture device of cultivateing microorganism |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205473757U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105670913A (en) * | 2016-03-30 | 2016-06-15 | 浙江大学 | Isolated culture device specific to microorganisms difficult to culture and method thereof |
-
2016
- 2016-03-30 CN CN201620255953.7U patent/CN205473757U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105670913A (en) * | 2016-03-30 | 2016-06-15 | 浙江大学 | Isolated culture device specific to microorganisms difficult to culture and method thereof |
| CN105670913B (en) * | 2016-03-30 | 2018-02-16 | 浙江大学 | It is a kind of to be separately cultured devices and methods therefor for difficult culture microorganism |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US9273278B2 (en) | Large scale cell harvesting method for pack-bed culture device | |
| RU2019138327A (en) | METHODS OF PERFUSION CULTIVATION AND THEIR APPLICATION | |
| CN205473757U (en) | To difficult separating culture device of cultivateing microorganism | |
| CN203212573U (en) | Device for improving culturability of microorganisms | |
| WO2014020215A1 (en) | Method for large-scale production of mycelial inoculum of ascomycota | |
| CN201245656Y (en) | 96 dot array drug sensitive test box | |
| CN105670913B (en) | It is a kind of to be separately cultured devices and methods therefor for difficult culture microorganism | |
| CN209636222U (en) | A kind of classifted training device of edaphon | |
| CN103667511A (en) | Kit for PCR (Polymerase Chain Reaction) detection of haemophilus paragallinarum | |
| CN105670914B (en) | It is a kind of can continuous supplementation factor of influence culture dish | |
| CN206328389U (en) | A kind of use for laboratory microbial culture dish for being easy to data record | |
| CN102676407A (en) | Microzyme for degrading residual lincomycin in fermented leaves and application thereof | |
| CN207047204U (en) | A kind of Enrichment by Microorganisms acquisition means | |
| CN204929995U (en) | A kind of warm canopy of hut cultivation Penaeus Vannmei | |
| CN103406163A (en) | Shaking incubator with four layers independent in temperature control | |
| CN204162724U (en) | A kind of perfusion cap cultivated for biological sample filling type | |
| CN207811763U (en) | A kind of container for placing culture medium | |
| CN205088245U (en) | Culture vessel | |
| CN202148305U (en) | Incubator | |
| KR20060112408A (en) | Culture dish with comparted-cells using same medium | |
| CN203400736U (en) | Four-layer type shaking cultivation box | |
| CN203913007U (en) | Novel fruit bat anesthesia outfit | |
| CN204434633U (en) | The structure of a kind of Tissue Culture Plate and plate lid thereof | |
| CN207713720U (en) | A kind of microbiological incubator | |
| CN111149729A (en) | Aquatic ecological breeding method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |