CN203582859U - Bacteria selector - Google Patents
Bacteria selector Download PDFInfo
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- CN203582859U CN203582859U CN201320414924.7U CN201320414924U CN203582859U CN 203582859 U CN203582859 U CN 203582859U CN 201320414924 U CN201320414924 U CN 201320414924U CN 203582859 U CN203582859 U CN 203582859U
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- choose
- bacterium device
- bacterium
- bacteria
- choosing
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Abstract
The utility model provides a bacteria selector, comprising a test tube type bacteria selector tube and a bacteria selector cover for sealing an opening of the bacteria selector tube. The bacteria selector is characterized in that a liquid medium is contained and stored in an inner cavity of the bacteria selector tube; a lengthwise bacteria selector needle is arranged at the inner side of the bacteria selector cover. The bacteria selector provided by the utility model is used for monoclonal colony selection and subsequent bacteria cultivation and detection in the bacteria cultivating process; all processes of bacteria colony selection, bacteria colony liquid cultivation, bacteria storage, and bacteria treatment and detection can be achieved by using the bacteria selector under the totally-enclosed condition; waste of other consumable items in the bacteria cultivation process is reduced, the labor cost is saved, the potential risks of environmental pollution and personal injury caused in the sample operation process are reduced, the work efficiency of the bacteria cultivation workers is improved, and the biosecurity of the bacteria cultivation process is improved.
Description
Technical field
The utility model patent relates to a kind of disposable use and chooses bacterium device.
Background technology
In molecular biology and microbiological fundamental research and experimental teaching, all can use microbionation and cultivation, in this process, the picking of mono-clonal bacterium colony and liquid culture are the most basic operations.Because, the representative of bacterium colony mono-clonal be same gene type, by a bacterium, constantly breed generation.Therefore, in the operation of and bacterium all in actication of culture and preservation, epidemiological study etc., all require picking mono-clonal bacterium colony to carry out liquid culture, preservation or other subsequent operations.
In the operation of mono-clonal bacterium colony picking, general working method is first to prepare bacterial liquid substratum now, by liquid nutrient medium autoclave sterilization, then sterilized liquid nutrient medium is divided and is installed in sterile test tube, the one capping teeth label of sterilizing simultaneously.While wanting picking mono-clonal bacterium colony with hand or take the photograph sub-folder and live toothpick picking mono-clonal bacterium colony on solid culture flat board.Also there are part operation personnel directly with the pipettor gun head of sterilizing, to carry out picking mono-clonal bacterium colony.Then by choosing the toothpick of mono-clonal bacterium colony or liquid nutrient medium that pipettor gun head is directly put into test tube, carry out enlarged culturing.After having cultivated, carry out follow-up bacterium operation and process.Finally the test tube being finished is carried out to autoclave sterilization and cleaning.
From schedule of operation above, can find out, the operating process of existing mono-clonal bacterium colony picking is very loaded down with trivial details, and link hand-manipulated is a lot, needs the material of preparation and sterilizing various.And because being directly puts into liquid nutrient medium by toothpick to cultivate, some materials in toothpick also can have a huge impact the growth of some bacteriums.Also easily cause the pollution of other miscellaneous bacteria to culturing bacterium in operating process, or the pollution of culturing bacterium to environment.
Therefore need to develop a kind of disposable use chooses bacterium device, can realize whole mono-clonal bacterium colony picking and culturing process totally closed operation, without preparing other experiment material.
Utility model content
Technical problem to be solved in the utility model is to provide a kind of can realize whole mono-clonal bacterium colony picking and culturing process totally closed operation, without the disposable use of preparing other experiment material, chooses bacterium device.
The invention provides a kind of bacterium device of choosing, it comprises choosing bacterium organ pipe and the described bacterium device of choosing of choosing the closure of openings of bacterium organ pipe being covered of test-tube type, it is characterized in that: described in choose in bacterium organ pipe inner chamber dress and have liquid nutrient medium, described in choose bacterium device lid inner side be provided with lengthwise choose bacterium device pin.
Preferably, the described bacterium device of choosing covers and is provided with a dripping hole running through, the described bacterium device lid outside of choosing is fixedly installed a broken latch, before described latch fractures by described dripping hole shutoff, after described latch fractures, expose described dripping hole, described in choose liquid in bacterium device and can ooze along the described dripping hole exposing.
Preferably, described latch is cylindrical, its near described in choose bacterium device lid one end be provided with the broken part caving inward, described dripping hole gos deep in described latch through described broken part along the axis of described latch.
Preferably, described in choose bacterium device lid inner side and be symmetrically arranged with six prismatic footpaths with the described central shaft of choosing bacterium device lid, described in choose bacterium device pin afterbody by shrink-fit, be removably disposed in the middle of described prismatic footpath, described in choose bacterium device pin front portion be tiny aciculiform.
Preferably, described in choose bacterium device lid inner side and be also provided with and choose bacterium device filter screen, described in choose on bacterium device filter screen, to be provided with described in the holding an of perforation and choose manhole and several micropores that bacterium device pin passes through.
Preferably, the diameter of described micropore is 0.6mm.
Preferably, described in, choose bacterium organ pipe and choose bacterium device lid and be threaded connection.
Preferably, described in choose bacterium device pin and described in choose the spacing that in bacterium organ pipe inner chamber, dress has between liquid nutrient medium and be greater than 0.5mm, and be less than 1.5mm, described in choose bacterium organ pipe material be plastics.
The purpose of this utility model is achieved by following technique means:
Choose a bacterium device, comprise and choose bacterium device pin, choose bacterium device lid, choose bacterium organ pipe and choose four parts of bacterium device filter screen.Choosing bacterium organ pipe is the test tube shape that plastics are made, choose bacterium device lid and choose bacterium organ pipe to be threaded connection, and an enclosed space of inner formation after being threaded, enclosed space is deposited liquid nutrient medium for dress.Choose thicker one of bacterium device pin and be fixed on and choose in bacterium device lid, one of needle-like, for mono-clonal bacterium colony picking, is chosen bacterium device pin from 1 millimeter of liquid nutrient medium liquid level.Choose bacterium device filter screen and be fixed on and choose in bacterium device lid, choose bacterium device filter screen and choose bacterium device pin and be all positioned at and choose bacterium device lid and choose the enclosed space that bacterium organ pipe is threaded and forms.Choosing bacterium device tops has one for the dripping hole of dropping liquid, and the top of choosing bacterium device lid outside is fixed with a latch that is easy to fracture, and when latch is intact, dripping hole can be blocked, and after latch is fractureed, dripping hole just can be opened.
Choose bacterium device in use, first filling a certain amount of liquid nutrient medium in choosing bacterium organ pipe can be preserved for a long time and be used after irradiation sterilization.When bacterium device is chosen in use, open and choose bacterium device lid, cover to be fixed with and choose bacterium device pin, with the mono-clonal bacterium colony of choosing on the head picking solid culture flat board of bacterium device pin, turn back to and choose in bacterium organ pipe, tighten and carry out liquid culture, latch fractures after having cultivated, from dripping hole, ooze detection or plasmid extraction that several culturing bacterium carry out next step, all the other continue reservation and use it for anything else.
The bacterium device of choosing of the present utility model can be realized whole mono-clonal bacterium colony picking and culturing process totally closed operation, without preparing other experiment materials such as sterilizing test tubes, sterilising liq substratum, sterilizing toothpick, also without to cleaning and sterilizing by the experiment material of mistake.Significantly reduce the operation steps of mono-clonal bacterium colony picking, improved scientific research personnel's working efficiency, saved labor cost.
Accompanying drawing explanation
Fig. 1 is the structural representation that the utility model is chosen bacterium device
Fig. 2 is the diagrammatic cross-section that the utility model is chosen bacterium device;
Fig. 3 is the structural representation of choosing bacterium organ pipe;
Fig. 4 is the diagrammatic cross-section of choosing bacterium device lid;
Fig. 5 is the upward view of Fig. 4;
Fig. 6 is the perspective view of choosing bacterium device pin;
Fig. 7 is the perspective view of choosing bacterium device filter screen.
Embodiment
In order to make the technical problems to be solved in the utility model, technical scheme and result of use more clear, below in conjunction with example, the utility model is further illustrated.Should be noted that specific examples described herein is only in order to explain the utility model, and be not used in restriction the utility model.
As shown in Fig. 1-7, the bacterium device of choosing of the present utility model comprises that choosing bacterium device covers 10, chooses bacterium device pin 12, chooses bacterium organ pipe 14, chooses bacterium device filter screen 16.Choose the aseptic liquid nutrient medium 18 through irradiation sterilization is housed in bacterium organ pipe 14, the add-on of aseptic liquid nutrient medium 18 is the position joining as shown in Figure 2, it is greater than 0.5mm with the height L that chooses bacterium device pin 12, and be less than 1.5mm, be preferably 1mm, do not contact with choosing bacterium device pin 12, to avoid being stained with the globule on pin and affect next step mono-clonal bacterium colony picking.Choose bacterium device lid 10 and choose bacterium organ pipe 14 and be threaded connection.
Choose bacterium device lid 10 and choose and on bacterium organ pipe 14, be provided with screw thread 19, choose an annular tongue 20 is set on bacterium organ pipe 14, the first half tube wall of choosing bacterium organ pipe 14 is relatively thick, quality is hard, transparency is poor, choose the Lower Half of bacterium organ pipe 14, tube wall is relatively thin, quality is softer, transparency is higher, after pinching with hand, extrudes culture.Choosing bacterium organ pipe 14 makes with the common plastics of industry or other materials.
The top of choosing bacterium device lid 10 arranges latch 22, and the broken part 24 of frangibility is set on latch 22.Choose on bacterium device lid 10 dripping hole 26 that bacterium organ pipe 14 inner chambers are chosen in one end connection is set, the other end of dripping hole 26 is crossed broken part 24 and is deep in latch 22.When latch 22 is intact, can seal dripping hole 26, avoid liquid to flow out from choosing the top of bacterium device lid 10, after latch 22 is fractureed, dripping hole 26 is opened, and the liquid culture of choosing in bacterium organ pipe 14 can be extruded and be detected operation by dripping hole 26.Choose the inner side that bacterium device covers upper part of 10 inner chambers and be provided with six prismatic footpaths 28 as shown in Figure 5, prismatic footpath 28 arranges to choose the central shaft annular of bacterium device lid 10, the centre hole forming is for inserting and fixedly choose bacterium device pin 12, can fix like this and choose bacterium organ pipe 14, can guarantee again that liquid culture can be easy to extrude to choose bacterium device lid 10.Choose bacterium device and cover lower parts of 10 inner chambers, for fixedly choosing bacterium device filter screen 16.Choose bacterium device and cover three prismatic footpaths 30 of 10 outer setting, play convenient finger power-assisted and tighten the effect of choosing bacterium device lid 10.
Choose 12 minutes three parts of bacterium device pin, progressively attenuate, comprising: the thickest portion 32, pars intermedia 34 and head 36.Wherein, the surface of the thickest portion 32 is also coarse, for being fixed on by six centre holes that rib footpath surrounds choosing on bacterium device lid 10.The head 36 of choosing bacterium device pin 12 is needle-like, and its diameter is 0.7mm, is convenient to picking mono-clonal bacterium colony.
Choose bacterium device filter screen 16 for the cylindrical shape of hollow, its one end sealing, the other end opening.The one end of choosing 16 sealings of bacterium device filter screen is provided with the aperture 38 of a lot of 0.6mm diameters, these apertures 38 allow passing through of water molecules and bacterial cell, but other thicker impurity in energy barrier liquid culture, thereby improve the detector efficiency in next step testing process.The central authorities of one end of sealing also have a manhole 40, and the diameter of through hole 40 and the same size of diameter of choosing the thickest portion 32 of bacterium device pin 12, be fixed in the centre hole of choosing six rib footpath formation on bacterium device lid 10 through through hole 40 thereby choose bacterium device pin 12.
When carrying out mono-clonal bacterium colony picking and cultivating with the utility model, first turn on and choose bacterium device lid 10, cover to be fixed with and choose bacterium device pin 12, it has been bacterium colony picking that the needle-like head 36 of choosing bacterium device pin 12 is aimed to bacterium mono-clonal bacterium colony on solid culture flat boards smears gently.Complete after mono-clonal bacterium colony picking, will choose bacterium device lid 10 and cover the bacterium organ pipe 14 that back-slabs, choose bacterium organ pipe 14 aseptic liquid nutrient medium 18 is housed in advance, therefore can directly carry out microbial culture.After having cultivated, from broken part, 24 latches 22 that fracture, expose dripping hole 26, to extrude a few drop of liquid cultures, carry out PCR detection, culture method inoculation or extracting genome DNA, and all the other cultures continue to save backup.
The foregoing is only preferred embodiment of the present utility model; not in order to limit the utility model; all any modifications of doing within spirit of the present utility model and principle, be equal to and replace and improvement etc., within all should being included in protection domain of the present utility model.
Claims (8)
1. choose a bacterium device, it comprise test-tube type choose bacterium organ pipe and by described closure of openings of choosing bacterium organ pipe choose bacterium device lid, it is characterized in that: described in choose dress in bacterium organ pipe inner chamber and have liquid nutrient medium, described in choose bacterium device lid inner side be provided with lengthwise choose bacterium device pin.
2. the bacterium device of choosing as claimed in claim 1, it is characterized in that: described in choose bacterium device and cover and be provided with a dripping hole running through, the described bacterium device lid outside of choosing is fixedly installed a broken latch, before described latch fractures by described dripping hole shutoff, after described latch fractures, expose described dripping hole, described in choose liquid in bacterium device and can ooze along the described dripping hole exposing.
3. the bacterium device of choosing as claimed in claim 2, it is characterized in that: described latch is cylindrical, its near described in choose bacterium device lid one end be provided with the broken part caving inward, described dripping hole gos deep in described latch through described broken part along the axis of described latch.
4. the bacterium device of choosing as claimed in claim 3, it is characterized in that: described in choose bacterium device lid inner side and be symmetrically arranged with six prismatic footpaths with the described central shaft of choosing bacterium device lid, the described afterbody of choosing bacterium device pin is removably disposed in by shrink-fit in the middle of described prismatic footpath, described in choose bacterium device pin front portion be tiny aciculiform.
5. the bacterium device of choosing as claimed in claim 4, is characterized in that: described in choose bacterium device lid inner side and be also provided with and choose bacterium device filter screen, described in choose on bacterium device filter screen, to be provided with described in the holding an of perforation and choose manhole and several micropores that bacterium device pin passes through.
6. the bacterium device of choosing as claimed in claim 5, is characterized in that: the diameter of described micropore is 0.6mm.
7. as claim 1-6 chooses bacterium device as described in one of them, it is characterized in that: described in choose bacterium organ pipe and choose bacterium device lid and be threaded connection.
8. the bacterium device of choosing as claimed in claim 7, is characterized in that: described in choose bacterium device pin and described in choose the spacing that in bacterium organ pipe inner chamber, dress has between liquid nutrient medium and be greater than 0.5mm, and be less than 1.5mm, described in choose bacterium organ pipe material be plastics.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320414924.7U CN203582859U (en) | 2013-07-12 | 2013-07-12 | Bacteria selector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320414924.7U CN203582859U (en) | 2013-07-12 | 2013-07-12 | Bacteria selector |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203582859U true CN203582859U (en) | 2014-05-07 |
Family
ID=50580806
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320414924.7U Expired - Fee Related CN203582859U (en) | 2013-07-12 | 2013-07-12 | Bacteria selector |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203582859U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112126586A (en) * | 2020-09-29 | 2020-12-25 | 安徽省金裕生物科技开发有限公司 | Bacterial colony picking device for wine making and use method thereof |
-
2013
- 2013-07-12 CN CN201320414924.7U patent/CN203582859U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112126586A (en) * | 2020-09-29 | 2020-12-25 | 安徽省金裕生物科技开发有限公司 | Bacterial colony picking device for wine making and use method thereof |
| CN112126586B (en) * | 2020-09-29 | 2023-08-18 | 安徽省金裕生物科技开发有限公司 | Colony picking device for brewing and application method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140507 Termination date: 20140712 |
|
| EXPY | Termination of patent right or utility model |