CN112126586B - Colony picking device for brewing and application method thereof - Google Patents
Colony picking device for brewing and application method thereof Download PDFInfo
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- CN112126586B CN112126586B CN202011052104.9A CN202011052104A CN112126586B CN 112126586 B CN112126586 B CN 112126586B CN 202011052104 A CN202011052104 A CN 202011052104A CN 112126586 B CN112126586 B CN 112126586B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
Abstract
The invention relates to the technical field of brewing, in particular to a colony picking device for brewing, which comprises a main body mechanism, a clamping mechanism and an oxygen removal mechanism, wherein the main body mechanism comprises a first fixing block, a first connecting block, a first spring, a main box body, a second fixing block, a second connecting block, a third connecting block, a first sealing cover, a fourth connecting block, a first scraping plate, a second spring, first traceless glue, a fifth connecting block, a sixth connecting block and second traceless glue, the two sides of the bottom end of the first fixing block are respectively fixed with the first spring, the bottom end of the first spring is fixed on the upper surface of the main box body, and the first connecting block is fixed at the central position of the bottom end of the first fixing block. The invention is provided with the deoxidizing mechanism, so that the operation is always in a state of less oxygen, the device can pick larger and smaller bacterial colonies through the main body mechanism and the clamping mechanism, and toothpicks and scraping plates are simultaneously placed in batches inside the fourth fixed block, so that the experimental process is simple and rapid.
Description
Technical Field
The invention relates to the technical field of brewing, in particular to a colony picking device for brewing and a using method thereof.
Background
With the continuous development of society, people who like to drink wine are more and more, in order to produce more favored wine, people are required to continuously improve the formula of the wine, in the wine formula experiment, bacteria are inoculated, cultivated and detected, when the existing bacterial colony is picked, an alcohol lamp is burnt on an operation table to enable the operation to be in a state with less oxygen, the cover of a solid culture dish is usually removed firstly, then a sterilized toothpick is clamped by a sterilizing treatment camera, or a pipette tip is used for picking the culture dish by the other hand, a monoclonal bacterial colony is picked up by a blunt end of the toothpick or a tip of the tip on the solid culture medium, and then the toothpick or the pipette tip with the monoclonal bacterial colony is directly put into a liquid culture medium in a test tube for expansion cultivation, and subsequent bacterial operation and treatment are carried out after the cultivation.
However, the existing operation needs to be performed at a short distance near the alcohol lamp, the operated appliances need to be placed near the alcohol lamp, the operation process is performed near the alcohol lamp, the operation difficulty is high, the operation steps are multiple, meanwhile, the colony picked by the toothpick is small, and when the larger colony needs to be picked, the experiment process is complicated and difficult due to the fact that other tools are matched with tweezers, so that a colony picking device for brewing wine and a using method thereof are needed to solve the problems.
Disclosure of Invention
The invention aims to provide a colony picking device for brewing and a using method thereof, which are used for solving the problems of high operation difficulty and complicated operation near an alcohol lamp in the background technology.
In order to achieve the above purpose, the present invention provides the following technical solutions: the utility model provides a bacterial colony picking device for making wine, includes main part mechanism, presss from both sides and gets mechanism and deoxidization mechanism, main part mechanism includes first fixed block, first connecting block, first spring, main tank body, second fixed block, second connecting block, third connecting block, first sealed lid, fourth connecting block, first scraper blade, second spring, first traceless glue, fifth connecting block, sixth connecting block and second traceless glue, the bottom both sides of first fixed block all are fixed with first spring, and the bottom mounting of first spring is in the upper surface of main tank body, the bottom central point department of first fixed block is fixed with first connecting block, the top both sides inner wall of main tank body is fixed with the second fixed block, and the inside of second fixed block is inserted and is equipped with first connecting block, the inside third connecting block that is equipped with of bottom of main tank body, and the top of third connecting block is fixed with the second connecting block, the bottom end of the third connecting block is fixedly connected with a fourth connecting block, the surface of the third connecting block is sleeved with a second spring, the inner wall of the main box body is fixedly provided with a fifth connecting block, one side, far away from the main box body, of the fifth connecting block is fixedly provided with a sixth connecting block, the inner wall of the sixth connecting block is connected with a second traceless glue, the surface of the main box body is connected with a clamping mechanism, the clamping mechanism comprises a third fixing block, a third spring, a toothpick, a fourth fixing block, a fifth fixing block, a sleeve, a scraping plate, a third traceless glue, a first baffle, a fourth spring and a sixth fixing block, the upper surface and the lower surface, close to the fourth fixing block, of the third fixing block are fixedly provided with the third spring, the third spring is fixedly arranged on the surface of the fourth fixing block, the fourth fixing block is fixedly arranged on the rear surface of the main box body, the fourth fixing block is provided with two groups, the inside of fourth fixed block of a set of insert is equipped with the toothpick, and another set of the inside of fourth fixed block is inserted and is equipped with scrapes the board of getting, the surface of toothpick is inconsistent with the surface of first baffle, and one side that the toothpick was kept away from to first baffle is fixed with the fourth spring, one side that the first baffle was kept away from to the fourth spring is fixed with the sixth fixed block, one side that the main tank was kept away from to the fourth fixed block is fixed with the sleeve pipe, and sheathed tube inside is inserted and is equipped with the fifth fixed block, the bottom both sides surface connection of main tank has deoxidization mechanism, deoxidization mechanism includes seventh fixed block, second sealed lid, third sealed lid, alcohol stove, eighth fixed block, pivot, dead lever, second baffle, wick and sealed maintenance door, the bottom inner wall fixed dead lever of seventh fixed block, and the dead lever is kept away from one side of main tank and is fixed with the alcohol stove, and the top fixed mounting of alcohol stove has the third sealed lid, one side that the third sealed lid is close to the alcohol stove is equipped with the wick, the bottom of the second sealed lid is kept away from the second sealed top of main tank, seven fixed blocks are kept away from the bottom fixed to the bottom of the main tank.
Preferably, the bottom of the first connecting block is internally provided with a cylindrical groove, and the inner wall of the cylindrical groove is fixedly connected with first traceless glue.
Preferably, a rectangular sliding groove is formed in the surface of the main box body, a second connecting block and a third connecting block are inserted into the sliding groove, and two groups of second connecting blocks and two groups of third connecting blocks are arranged.
Preferably, the second fixing blocks are symmetrically distributed on the inner walls of the two sides of the main box body, and the second fixing blocks are of a semi-annular structure in overlook view.
Preferably, the fourth connecting block is provided with two groups, and the fourth connecting block is semi-annular structure, the inner wall of fourth connecting block is connected with first scraper blade, and first scraper blade is semi-annular structure.
Preferably, the third springs are provided with two groups, each group is provided with two groups, and two sides of the third springs are respectively fixed on the surfaces of the third fixing block and the fourth fixing block.
Preferably, the toothpicks are uniformly distributed inside one group of the fourth fixed block at equal angles, the diameter of the outer wall of the toothpicks is equal to that of the inner wall of the fourth fixed block, the bottom end of the scraping plate is of a trapezoid structure, and the scraping plate is uniformly distributed inside the other group of the fourth fixed block at equal angles.
Preferably, the fifth fixing blocks are symmetrically distributed inside two sides of the main box body, an arc-shaped groove is formed in the back face of the fifth fixing block, and third traceless glue is connected to the inner wall of the groove.
Preferably, the second baffle is of a trapezoid structure, a lamp wick is inserted into the inner center of the second baffle, and the second baffle and the lamp wick are symmetrically arranged at two sides of the bottom end of the main box body.
The application method of the colony picking device for brewing comprises the following steps:
the first step: the first sealing cover is disassembled in a rotating way, a toothpick or a scraping plate is selected according to the size of a colony, a larger colony is selected, a scraping plate is selected, a smaller colony is selected, a toothpick is selected, and the smaller colony is selected by the toothpick;
and a second step of: igniting one or two groups of lampwicks according to the time for picking the bacterial colony, picking the bacterial colony for brewing under the state of less oxygen, and continuously burning the lampwick to ensure that the bottom end of the main box body is in the state of very less oxygen, thereby meeting the environment for picking the bacterial colony;
and a third step of: pressing a third fixing block to the inner side of the main box body, continuously extruding the toothpick by the first baffle under the elasticity of a fourth spring, adhering the toothpick to the surface of the third traceless glue, pressing the third fixing block to one side of the main box body, compressing the third spring, driving the fifth fixing block and the third traceless glue to move into the main box body by the third fixing block, limiting the third fixing block by a sleeve, stopping the toothpick from moving to the position of the second traceless glue, adhering the toothpick again by the second traceless glue, and fixing the toothpick;
fourth step: after the toothpick is fixed in position, the first fixing block is pressed downwards, the first spring is compressed, the bottom end of the first connecting block is directly sleeved on the surface of the toothpick and is fixed with the toothpick through the first traceless glue, the third fixing block is loosened, the third fixing block is restored to the original position under the elasticity of the third spring, and the next group of toothpicks are stuck together with the third traceless glue again under the extrusion of the first baffle;
fifth step: continuously pressing the first fixing block downwards, wherein the first spring drives the toothpick to move downwards and separate from the position of the second traceless glue, the first fixing block drives the toothpick to extend out of the bottom end of the main box body, and the toothpick can directly pick out bacterial colonies;
sixth step: after the colony is picked, the colony can adhere on the surface of the toothpick and is not easy to fall in the container, the second connecting block is pressed downwards, the second connecting block drives the third connecting block and the fourth connecting block to move downwards, the first scraping plate pushes down the colony on the surface of the toothpick, the colony is convenient to fall, the lamp core is killed after the device is used, and then the device is disinfected and sterilized, so that the device is convenient to use again.
Compared with the prior art, the invention has the beneficial effects that: this making wine is with bacterial colony picking device is provided with deoxidization mechanism, and the deoxidization mechanism on the device moves along with the operation process, and the operation degree of difficulty is little, and it is big and less bacterial colony to get the mechanism messenger device through main part mechanism and clamp, places toothpick and scrapes the board in batches simultaneously through the inside fourth fixed block, makes the experimental process become simple swift.
(1) The device is provided with deoxidization mechanism, and deoxidization mechanism installs in the bottom both sides of device, ignites the wick and makes the device be in under the less state of oxygen always, and the utensil that need not will operate is near the alcohol burner, and the deoxidization mechanism on the device moves along with the operation process, and the operation degree of difficulty is little, makes things convenient for experimental operation, and the cooperation of getting the mechanism through main part mechanism and clamp is used, and the toothpick is different with the area of scraping the board bottom, makes the device can pick bigger and less colony, and the application range of device is extensive.
(2) The device is inside place toothpick and scrape and get the board in batches simultaneously through the fourth fixed block, does not need the manual work to press from both sides many times and get the use, does not need to use through other instrument cooperation tweezers, makes the experimental process become simple swift, and the colony that conveniently with toothpick and scrape and get board surface adhesion pushes away down through second connecting block and third connecting block, makes the colony pick up fast.
Drawings
FIG. 1 is a schematic elevational cross-sectional view of the structure of the present invention;
FIG. 2 is an enlarged schematic view of the structure A in FIG. 1 according to the present invention;
FIG. 3 is a schematic elevational view of the structure of the present invention;
FIG. 4 is a bottom view of a first connecting block according to the present invention;
FIG. 5 is a schematic top view of the third and fourth connection block arrangements of the present invention;
FIG. 6 is a schematic top view of a gripping mechanism of the present invention;
fig. 7 is a schematic top view of the clamping mechanism of the present invention.
In the figure: 100. a main body mechanism; 110. a first fixed block; 120. a first connection block; 130. a first spring; 140. a main case; 150. a second fixed block; 160. a second connection block; 170. a third connecting block; 180. a first sealing cover; 190. a fourth connecting block; 1100. a first scraper; 1110. a second spring; 1120. a first traceless glue; 1130. a fifth connecting block; 1140. a sixth connecting block; 1150. a second traceless glue; 200. a clamping mechanism; 210. a third fixed block; 220. a third spring; 230. a toothpick; 240. a fourth fixed block; 250. a fifth fixed block; 260. a sleeve; 270. a scraping plate; 280. a third traceless glue; 290. a first baffle; 2100. a fourth spring; 2110. a sixth fixed block; 300. an oxygen removal mechanism; 310. a seventh fixed block; 320. a second sealing cover; 330. a third sealing cover; 340. an alcohol burner; 350. an eighth fixed block; 360. a rotating shaft; 370. a fixed rod; 380. a second baffle; 390. a wick; 3100. sealing the maintenance door.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1-7, an embodiment of the present invention is provided:
the utility model provides a bacterial colony picking device for making wine, including main part mechanism 100, press from both sides and get mechanism 200 and deoxidization mechanism 300, main part mechanism 100 includes first fixed block 110, first connecting block 120, first spring 130, main box 140, second fixed block 150, second connecting block 160, third connecting block 170, first sealed lid 180, fourth connecting block 190, first scraper blade 1100, second spring 1110, first no trace glue 1120, fifth connecting block 1130, sixth connecting block 1140 and second no trace glue 1150, the bottom both sides of first fixed block 110 all are fixed with first spring 130, and the bottom mounting of first spring 130 is in the upper surface of main box 140, the bottom central point department of first fixed block 110 is fixed with first connecting block 120, cylindrical recess has been seted up to the inside bottom of first connecting block 120, and cylindrical recess's inner wall fixedly connected with first no trace glue 1120, cylindrical recess makes things convenient for toothpick 230 or scrapes and gets board 270 to insert, first no trace glue 1120 can fix toothpick 230 or scrape and get board 270.
The inner walls of the two sides of the top end of the main box body 140 are fixedly provided with second fixing blocks 150, the surface of the main box body 140 is provided with rectangular sliding grooves, the second connecting blocks 160 and the third connecting blocks 170 are inserted into the sliding grooves, the second connecting blocks 160 and the third connecting blocks 170 are respectively provided with two groups, the two groups of third connecting blocks 170 can simultaneously adjust positions through the two groups of second connecting blocks 160, the second fixing blocks 150 are symmetrically distributed on the inner walls of the two sides of the main box body 140, the second fixing blocks 150 are in a semi-annular structure in a overlook view, the second fixing blocks 150 are sleeved on the surface of the first connecting blocks 120, the first connecting blocks 120 can conveniently slide in the second fixing blocks 150, the inside of second fixed block 150 is inserted and is equipped with first connecting block 120, the inside third connecting block 170 that is equipped with of bottom of main tank 140, and the top of third connecting block 170 is fixed with second connecting block 160, the bottom fixedly connected with fourth connecting block 190 of third connecting block 170, fourth connecting block 190 is provided with two sets of, and fourth connecting block 190 is semi-annular structure, fourth connecting block 190 can overlap the surface at toothpick 230 or scrape the board 270, the inner wall of fourth connecting block 190 is connected with first scraper 1100, and first scraper 1100 is semi-annular structure, first scraper 1100 can scrape the colony of toothpick 230 or scrape the adhesion of board 270 surface, the convenience is operated next step to the colony.
The surface of the third connecting block 170 is sleeved with a second spring 1110, the inner wall of the main box 140 is fixed with a fifth connecting block 1130, one side, far away from the main box 140, of the fifth connecting block 1130 is fixed with a sixth connecting block 1140, the inner wall of the sixth connecting block 1140 is connected with a second traceless glue 1150, the surface of the main box 140 is connected with a clamping mechanism 200, the clamping mechanism 200 comprises a third fixing block 210, a third spring 220, a toothpick 230, a fourth fixing block 240, a fifth fixing block 250, a sleeve 260, a scraping plate 270, a third traceless glue 280, a first baffle 290, a fourth spring 2100 and a sixth fixing block 2110, the upper and lower surfaces, close to the fourth fixing block 240, of the third fixing block 210 are respectively fixed with a third spring 220, the third springs 220 are provided with two groups, two sides of the third spring 220 are respectively fixed on the surfaces of the third fixing block 210 and the fourth fixing block 240, and the third spring 220 enables the third fixing block 210 to stably move in position to help the original position of the third fixing block 210.
The third spring 220 is fixed on the surface of the fourth fixed block 240, the fourth fixed block 240 is fixed on the rear surface of the main box 140, two groups of toothpicks 230 are inserted in one group of fourth fixed blocks 240, scraping plates 270 are inserted in the other group of fourth fixed blocks 240, the toothpicks 230 are uniformly distributed in one group of fourth fixed blocks 240 at equal angles, the diameter of the outer wall of each toothpick 230 is identical to the diameter of the inner wall of each fourth fixed block 240, the toothpicks 230 are in contact with the back surface of the fifth fixed block 250, the toothpicks 230 are conveniently adhered to the surface of the third traceless glue 280, the bottom ends of the scraping plates 270 are in a trapezoid structure, the scraping plates 270 are uniformly distributed in the other group of fourth fixed blocks 240, the bottom ends of the scraping plates 270 can pick more colonies, the surface of each toothpick 230 is in contact with the surface of the first baffle 290, and one side, far away from each toothpick 230, of the first baffle 290 is fixedly provided with the fourth spring 2100.
The fourth spring 2100 is fixed with sixth fixed block 2110 far away from one side of first baffle 290, the fourth fixed block 240 is fixed with sleeve 260 far away from one side of main tank 140, and the inside of sleeve 260 is inserted and is equipped with fifth fixed block 250, fifth fixed block 250 symmetric distribution is inside the both sides of main tank 140, the arc recess has been seted up at the back of fifth fixed block 250, and the inner wall of recess is connected with third traceless glue 280, the arc recess makes things convenient for toothpick 230 or scrape the board 270 and gets into the recess inside, the surface at third traceless glue 280 is convenient for the adhesion, the bottom both sides surface of main tank 140 is connected with deoxidization mechanism 300, deoxidization mechanism 300 includes seventh fixed block 310, second sealed lid 320, third sealed lid 330, alcohol burner 340, eighth fixed block 350, pivot 360, dead lever 370, second baffle 380, 390 and sealed maintenance door 3100, the bottom inner wall of seventh fixed block 310 is fixed with dead lever 370, and one side inside of keeping away from main tank 140 is inserted and is equipped with pivot 360.
The alcohol stove 340 is fixed with in one side that pivot 360 kept away from main tank body 140, and alcohol stove 340's top fixed mounting has the sealed lid 330 of third, the inside wick 390 that inserts in one side that the sealed lid 330 is close to main tank body 140, the sealed lid 320 of second is installed on one side top that alcohol stove 340 kept away from main tank body 140, the bottom mounting that the bottom that the seventh fixed block 310 is close to main tank body 140 has second baffle 380, second baffle 380 is trapezoidal structure, and the inside central point department of second baffle 380 inserts and has wick 390, second baffle 380 and wick 390 all symmetry are in the bottom both sides of main tank body 140, third traceless glue 280 blocks the flame that wick 390 burns, avoid damaging other positions of device, the one side bottom that the seventh fixed block 310 kept away from main tank body 140 is fixed with eighth fixed block 350, seventh fixed block 310's fixed surface mounting has sealed maintenance door 3100.
The application method of the colony picking device for brewing comprises the following steps:
the first step: rotating and disassembling the first sealing cover 180, selecting a toothpick 230 or a scraping plate 270 according to the size of a colony, selecting a larger colony, selecting a scraping plate 270, selecting a smaller colony, selecting a toothpick 230, and selecting a smaller colony by using the toothpick 230;
and a second step of: one or two groups of lampwicks 390 are ignited according to the length of time for picking the bacterial colony, the bacterial colony for brewing wine needs to be picked in a state of less oxygen, and the lampwick 390 is continuously burnt to enable the bottom end of the main box body 140 to be in a state of very less oxygen, so that the bacterial colony picking environment is met;
and a third step of: the third fixing block 210 is pressed towards the inner side of the main case 140, the first baffle 290 continuously extrudes the toothpick 230 under the elasticity of the fourth spring 2100, the toothpick 230 is adhered to the surface of the third traceless glue 280, the third fixing block 210 is pressed towards one side of the main case 140, the third spring 220 is compressed, the third fixing block 210 drives the fifth fixing block 250 and the third traceless glue 280 to move into the main case 140, the sleeve 260 limits the third fixing block 210, so that the toothpick 230 is stopped at the position of the second traceless glue 1150, the second traceless glue 1150 is adhered to the toothpick 230 again, and the position of the toothpick 230 is fixed;
fourth step: after the position of the toothpick 230 is fixed, the first fixing block 110 is pressed downwards, the first spring 130 is compressed, the bottom end of the first connecting block 120 is directly sleeved on the surface of the toothpick 230 and fixes the toothpick 230 through the first traceless glue 1120, the third fixing block 210 is loosened, the third fixing block 210 is restored to the original position under the elasticity of the third spring 220, and the next group of toothpicks 230 are adhered together with the third traceless glue 280 again under the extrusion of the first baffle 290;
fifth step: continuing to press the first fixing block 110 downwards, the first spring 130 drives the toothpick 230 to move downwards and separate from the position of the second traceless glue 1150, the first fixing block 110 drives the toothpick 230 to extend out of the bottom end of the main box body 140, and the toothpick 230 can directly pick out colonies;
sixth step: after the colony is picked, the colony can be adhered to the surface of the toothpick 230 and is not easy to fall in the container, the second connecting block 160 is pressed downwards, the second connecting block 160 drives the third connecting block 170 and the fourth connecting block 190 to move downwards, the first scraper 1100 pushes down the colony on the surface of the toothpick 230, the colony is convenient to fall, the lamp wick 390 is killed after the device is used, and then the device is disinfected and sterilized, so that the device is convenient to reuse.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (6)
1. A bacterial colony picking device for brewing wine is characterized in that: including main part mechanism (100), clamp get mechanism (200) and deoxidization mechanism (300), main part mechanism (100) include first fixed block (110), first connecting block (120), first spring (130), main tank body (140), second fixed block (150), second connecting block (160), third connecting block (170), first sealed lid (180), fourth connecting block (190), first scraper blade (1100), second spring (1110), first traceless glue (1120), fifth connecting block (1130), sixth connecting block (1140) and second traceless glue (1150), the bottom both sides of first fixed block (110) all are fixed with first spring (130), and the bottom fixing of first spring (130) is in the upper surface of main tank body (140), bottom central point department of first fixed block (110) is fixed with first connecting block (120), the top both sides inner wall of main tank body (140) is fixed with second fixed block (150), and the inside of second fixed block (150) is inserted and is equipped with first connecting block (120), the bottom of third fixed with third connecting block (170) and the top of third connecting block (170), the surface of the third connecting block (170) is sleeved with a second spring (1110), the inner wall of the main box body (140) is fixedly provided with a fifth connecting block (1130), one side, far away from the main box body (140), of the fifth connecting block (1130) is fixedly provided with a sixth connecting block (1140), the inner wall of the sixth connecting block (1140) is connected with a second traceless glue (1150), the surface of the main box body (140) is connected with a clamping mechanism (200), the clamping mechanism (200) comprises a third fixing block (210), a third spring (220), a toothpick (230), a fourth fixing block (240), a fifth fixing block (250), a sleeve (260), a scraping plate (270), a third traceless glue (280), a first baffle (290), a fourth spring (2100) and a sixth fixing block (2110), the upper surface and the lower surface of the third fixing block (210) close to the fourth fixing block (240) are respectively fixedly provided with a third spring (220), the surface of the third spring (220) is fixedly arranged on the fourth fixing block (240), the surface of the main box body (240) is provided with a toothpick (240), the other group of the fourth fixing block (240) is internally provided with another scraping plate (240), the surface of toothpick (230) is inconsistent with the surface of first baffle (290), and one side that toothpick (230) was kept away from to first baffle (290) is fixed with fourth spring (2100), one side that first baffle (290) was kept away from to fourth spring (2100) is fixed with sixth fixed block (2110), one side that main box (140) was kept away from to fourth fixed block (240) is fixed with sleeve (260), and the inside of sleeve (260) is inserted and is equipped with fifth fixed block (250), the bottom both sides surface of main box (140) is connected with deoxidization mechanism (300), deoxidization mechanism (300) include seventh fixed block (310), second sealed lid (320), third sealed lid (330), alcohol stove (340), eighth fixed block (350), pivot (360), dead lever (370), second baffle (380), wick (390) and sealed maintenance door (3100), the bottom inner wall of seventh fixed block (310) is fixed with dead lever (370), and one side that main box (140) was kept away from to dead lever (370) is inside is equipped with insert and is equipped with fifth fixed block (360), pivot (340) are kept away from main box (140) and are fixed with top end (340) and are kept away from to pivot (340), a lamp wick (390) is inserted into one side of the third sealing cover (330) close to the main box body (140), a second sealing cover (320) is installed at the top end of one side of the alcohol burner (340) far away from the main box body (140), a second baffle (380) is fixed at the bottom end of the seventh fixing block (310) close to the main box body (140), an eighth fixing block (350) is fixed at the bottom end of one side of the seventh fixing block (310) far away from the main box body (140), and a sealing maintenance door (3100) is fixedly installed on the surface of the seventh fixing block (310);
a cylindrical groove is formed in the bottom end of the first connecting block (120), and the inner wall of the cylindrical groove is fixedly connected with first traceless glue (1120);
the four connecting blocks (190) are provided with two groups, the four connecting blocks (190) are of semi-annular structures, the inner wall of each fourth connecting block (190) is connected with a first scraping plate (1100), and the first scraping plates (1100) are of semi-annular structures;
two groups of third springs (220) are arranged, each group is provided with two springs, and two sides of the third springs (220) are respectively fixed on the surfaces of a third fixed block (210) and a fourth fixed block (240);
the fifth fixed blocks (250) are symmetrically distributed inside two sides of the main box body (140), arc-shaped grooves are formed in the back surfaces of the fifth fixed blocks (250), and third traceless glue (280) is connected to the inner walls of the grooves.
2. The colony picking apparatus for brewing as claimed in claim 1, wherein: rectangular sliding grooves are formed in the surface of the main box body (140), a second connecting block (160) and a third connecting block (170) are inserted into the sliding grooves, and two groups of the second connecting block (160) and the third connecting block (170) are arranged.
3. The colony picking apparatus for brewing as claimed in claim 1, wherein: the second fixing blocks (150) are symmetrically distributed on the inner walls of the two sides of the main box body (140), and the second fixing blocks (150) are of a semi-annular structure in overlook view.
4. The colony picking apparatus for brewing as claimed in claim 1, wherein: the toothpick (230) is uniformly distributed inside one group of the fourth fixed block (240) at equal angles, the diameter of the outer wall of the toothpick (230) is the same as that of the inner wall of the fourth fixed block (240), the bottom end of the scraping plate (270) is of a trapezoid structure, and the scraping plate (270) is uniformly distributed inside the other group of the fourth fixed block (240) at equal angles.
5. The colony picking apparatus for brewing as claimed in claim 1, wherein: the second baffle (380) is of a trapezoid structure, a lamp wick (390) is inserted into the inner center of the second baffle (380), and the second baffle (380) and the lamp wick (390) are symmetrically arranged on two sides of the bottom end of the main box body (140).
6. The method of using a colony picking device for brewing according to any of claims 1-5, wherein: the method comprises the following steps:
the first step: the first sealing cover (180) is disassembled in a rotating mode, a toothpick (230) or a scraping plate (270) is selected to be used according to the size of a colony, the scraping plate (270) is selected to be used for picking a larger colony, the toothpick (230) is selected to be used for picking a smaller colony, and the toothpick (230) is used for picking a smaller colony;
and a second step of: igniting one or two groups of lampwicks (390) according to the time for picking the bacterial colony, picking the bacterial colony under the state of less oxygen, and continuously burning the lampwick (390) to ensure that the bottom end of the main box body (140) is in the state of very less oxygen, thereby meeting the environment for picking the bacterial colony;
and a third step of: pressing the third fixing block (210) towards the inner side of the main box body (140), continuously extruding the toothpick (230) by the first baffle plate (290) under the elasticity of the fourth spring (2100), adhering the toothpick (230) to the surface of the third traceless glue (280), pressing the third fixing block (210) towards one side of the main box body (140), compressing the third spring (220), driving the fifth fixing block (250) and the third traceless glue (280) to move into the main box body (140) by the third fixing block (210), limiting the third fixing block (210) by the sleeve (260), stopping the toothpick (230) from moving to the position of the second traceless glue (1150), adhering the toothpick (230) again by the second traceless glue (1150), and fixing the toothpick (230) in position;
fourth step: after the toothpick (230) is fixed in position, the first fixing block (110) is pressed downwards, the first spring (130) is compressed, the bottom end of the first connecting block (120) is directly sleeved on the surface of the toothpick (230) and fixes the toothpick (230) through the first traceless glue (1120), the third fixing block (210) is loosened, the third fixing block (210) is restored to the original position under the elasticity of the third spring (220), and the next group of toothpicks (230) are adhered together with the third traceless glue (280) again under the extrusion of the first baffle (290);
fifth step: continuously pressing the first fixing block (110) downwards, the first spring (130) drives the toothpick (230) to move downwards and separate from the position of the second traceless glue (1150), the first fixing block (110) drives the toothpick (230) to extend out of the bottom end of the main box body (140), and the toothpick (230) can directly pick out colonies;
sixth step: after the colony is picked, the colony can be adhered to the surface of the toothpick (230) and is not easy to fall in a container, the second connecting block (160) is pressed downwards, the second connecting block (160) drives the third connecting block (170) and the fourth connecting block (190) to move downwards, the first scraping plate (1100) pushes down the colony on the surface of the toothpick (230), the colony is convenient to fall, the lamp core (390) is killed after the device is used, and then the device is disinfected and sterilized, so that the device is convenient to reuse.
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