CN220116540U - Solid fungus piece sampling device - Google Patents
Solid fungus piece sampling device Download PDFInfo
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- CN220116540U CN220116540U CN202321560807.1U CN202321560807U CN220116540U CN 220116540 U CN220116540 U CN 220116540U CN 202321560807 U CN202321560807 U CN 202321560807U CN 220116540 U CN220116540 U CN 220116540U
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- spring
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- 238000005070 sampling Methods 0.000 title claims abstract description 105
- 241000233866 Fungi Species 0.000 title claims abstract description 38
- 239000007787 solid Substances 0.000 title claims abstract description 31
- 210000001503 joint Anatomy 0.000 claims description 6
- 238000013461 design Methods 0.000 claims description 2
- 238000006073 displacement reaction Methods 0.000 claims description 2
- 230000000813 microbial effect Effects 0.000 abstract description 3
- 238000000034 method Methods 0.000 description 6
- 238000011081 inoculation Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
In order to overcome the problems of complicated and easy pollution in the prior art of microbial block sampling, the utility model provides a solid microbial block sampling device, which comprises an inner cylinder, a first spring, a connecting rod, a sampling sleeve and a sampling nail, wherein the first spring is arranged in the inner cylinder, the first spring is connected with the connecting rod, the connecting rod stretches into and passes through the bottom of the inner cylinder from the top of the inner cylinder, the connecting rod can move back and forth between a first position and a second position, and the first spring is compressed when the connecting rod is forced to move to the second position; when the stress of the connecting rod is eliminated, the first spring is restored and drives the connecting rod to return to the first position. According to the solid fungus block sampling device provided by the utility model, the sampling sleeve and the sampling nail are matched, so that the sizes of sampling fungus blocks are consistent, the experimental accuracy is improved, and meanwhile, the sampling sleeve and the sampling nail can be disassembled, sterilized and disinfected after being used, so that the problem of easy pollution generated in fungus block sampling is relieved or avoided; the sampling device is simple and convenient to operate, and is particularly suitable for sampling various and a plurality of strains.
Description
Technical Field
The utility model belongs to the technical field of biological experimental instruments, and particularly relates to a solid fungus block sampling device.
Background
In microbial cultures and counter tests, it is often necessary to sample the pieces of bacteria on a plate, which is often the case during the culture of filamentous fungi; in practical operation of fungus block sampling, three methods are commonly used, namely, a inoculation shovel is used for taking fungus blocks, the disadvantage is that the obtained fungus blocks are different in size, a puncher is used for taking fungus blocks, the disadvantage is that fungus blocks which are not suitable for simultaneously taking different strains are easy to adhere to the puncher and are difficult to take out, a stainless steel short pipe is clamped by forceps, a round fungus cake is poked on an agar flat plate, and a sterile glass rod or a liquid-transferring gun head is used for poking the fungus blocks when the stainless steel short pipe connected with the fungus cake is placed on a new flat plate; the third method is a common fungus block sampling mode, but the method has the defects that the operation is complicated, the pollution probability is increased and obvious defects exist due to the fact that the flat plate is exposed to the air for a long time, so that the fungus block sampler device is convenient and safe to operate.
Disclosure of Invention
The utility model aims to solve the technical problems of complicated and easy pollution of microbial block sampling in the prior art and provides a solid block sampling device.
The technical scheme adopted by the utility model for solving the technical problems is as follows:
the utility model provides a solid fungus piece sampling device, including inner tube, first spring, connecting rod, sample sleeve pipe and sample nail, first spring sets up in the inner tube, first spring with the connecting rod is connected, the connecting rod stretches into from the top of inner tube and passes the inner tube bottom, the connecting rod can reciprocate between first position and second position, when the connecting rod atress moves to the second position, first spring is compressed; when the stress of the connecting rod disappears, the first spring is restored and drives the connecting rod to return to the first position; the bottom of connecting rod with the nail can dismantle the connection, the inner tube bottom is provided with the adapter, sampling sleeve can dismantle connect in on the adapter, by the connecting rod drives the nail of drawing a design wears out sampling sleeve or holding in the sampling sleeve.
Optionally, the sampling sleeve comprises a large sleeve and a small sleeve, the large sleeve and the small sleeve are coaxially arranged and connected with each other, the inner diameter of the large sleeve is larger than that of the small sleeve, and the large sleeve is sleeved with the adapter sleeve.
Optionally, the thickness of the small sleeve pipe wall is 0.6-1.0 mm.
Optionally, the proofing nail includes hollow tube and nail piece, the nail piece is solid structure, nail piece lid seals the one end mouth of pipe of hollow tube, the other end mouth of pipe detachably cover of hollow tube is located on the connecting rod.
Optionally, when the connecting rod is in the first position, a distance from the bottom of the adapter to the bottom of the connecting rod is greater than the length of the hollow tube, and the bottom of the connecting rod does not protrude out of the sampling sleeve; when the connecting rod is in the second position, the bottom of the connecting rod protrudes out of the sampling sleeve.
Optionally, the piston further comprises a piston and a first pressing block, a through hole is formed in the center of the piston, the top of the connecting rod is connected with the first pressing block, the connecting rod penetrates through the through hole and is fixedly connected with the piston, and the first spring is abutted to the piston.
Optionally, the removing device further comprises an outer cylinder and a second spring, wherein the outer cylinder is sleeved on the periphery of the inner cylinder in an axial displacement manner, the bottom of the outer cylinder is abutted against the sampling sleeve, and when the outer cylinder moves downwards under external force, the outer cylinder drives the sampling sleeve to be removed from the adapter; when the stress of the outer cylinder disappears, the second spring drives the outer cylinder to return.
Optionally, still include push rod, snap ring and handle, be provided with the second on the snap ring and press the briquetting, the second press the briquetting with push rod top butt, the bottom of push rod with second spring butt, the outer wall of inner tube is provided with supporting part, the bottom butt of second spring in on the supporting part, the snap ring encircle set up in the top of urceolus, the handle with snap ring one end is connected.
Optionally, the bottom of the outer cylinder is conical, and the handle is semicircular.
Optionally, the inner cylinder, the adapter and the sampling sleeve are coaxially arranged.
According to the solid fungus block sampling device provided by the utility model, the sampling sleeve and the proofing nail are matched, and then fungus block sampling and fungus block placement are carried out by utilizing the first spring and the connecting rod in the inner cylinder, so that the size of the sampling fungus block is consistent, the experimental accuracy is improved, and meanwhile, the sampling sleeve and the proofing nail can be disassembled, disinfected and sterilized after being used, so that the problem of easy pollution in fungus block sampling is relieved or avoided; the sampling device provided by the utility model is simple and convenient to operate, and is especially suitable for sampling various and numerous strains.
Drawings
FIG. 1 is an assembly structure diagram of a solid fungus block sampling device provided by the utility model in a natural state;
FIG. 2 is an assembly structure diagram of the solid fungus block sampling device provided by the utility model in a compressed state;
FIG. 3 is a schematic diagram of a sampling sleeve of the solid fungus block sampling device provided by the utility model;
fig. 4 is a schematic diagram of a sample-taking nail structure of the solid fungus block sampling device provided by the utility model.
Reference numerals in the drawings of the specification are as follows:
1. an inner cylinder; 2. a first spring; 3. a connecting rod; 4. sampling sleeve; 41. a large sleeve; 42. a small sleeve; 5. a sample drawing nail; 51. a hollow tube; 52. nailing sheets; 6. an adapter; 7. a piston; 8. a first pressing block; 9. a removal device; 10. a push rod; 11. a second spring; 12. an outer cylinder; 13. a clasp; 131. a second pressing block; 14. a handle.
Detailed Description
In order to make the technical problems, technical schemes and beneficial effects solved by the utility model more clear, the utility model is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the utility model.
In the description of the present utility model, it should be understood that the terms "longitudinal," "radial," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate orientations or positional relationships that are based on the orientation or positional relationships shown in the drawings, merely to facilitate describing the present utility model and simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model. In the description of the present utility model, unless otherwise indicated, the meaning of "a plurality" is two or more.
In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.
Referring to fig. 1 to 4, an embodiment of the present utility model provides a solid fungus block sampling device, including an inner cylinder 1, a first spring 2, a connecting rod 3, a sampling sleeve 4 and a sample nail 5, wherein the first spring 2 is disposed in the inner cylinder 1, the first spring 2 is connected with the connecting rod 3, the connecting rod 3 extends into and passes through the bottom of the inner cylinder 1 from the top of the inner cylinder 1, the connecting rod 3 can reciprocate between a first position and a second position, and when the connecting rod 3 is forced to move to the second position, the first spring 2 is compressed; when the stress of the connecting rod 3 disappears, the first spring 2 recovers and drives the connecting rod 3 to return to the first position; the bottom of the connecting rod 3 is detachably connected with the proofing nail 5, an adapter 6 is arranged at the bottom of the inner cylinder 1, the sampling sleeve 4 is detachably connected to the adapter 6, and the proofing nail 5 is driven by the connecting rod 3 to penetrate out of the sampling sleeve 4 or be accommodated in the sampling sleeve 4.
When the stress of the connecting rod 3 is eliminated, the sampling sleeve 4 is connected to the adapter 6; under the state that the connecting rod 3 is stressed and moved, the connecting rod 3 extends out of the sampling sleeve 4, the proofing nails 5 are sleeved on the connecting rod 3, and the proofing nails 5 are assembled.
In an embodiment, the sampling sleeve 4 includes a large sleeve 41 and a small sleeve 42, the large sleeve 41 and the small sleeve 42 are coaxially disposed and connected to each other, the inner diameter of the large sleeve 41 is larger than the inner diameter of the small sleeve 42, and the large sleeve 41 is sleeved with the adapter 6.
Specifically, in the preferred embodiment, the inner diameter of the small sleeve 42 is less than or equal to 6mm, and since the large sleeve 41 is sleeved on the adapter 6, the inner diameter of the large sleeve 41 is set according to the outer diameter of the adapter 6.
In one embodiment, the thickness of the wall of the small sleeve 42 is 0.6-1.0 mm.
The small cannula 42 is used to cut solid pieces of bacteria, so the thickness of the small cannula 42 wall is based on the ability to cut off the agar surface culture.
In an embodiment, the proofing nail 5 includes a hollow tube 51 and a nail piece 52, the nail piece 52 is of a solid structure, the nail piece 52 covers one end orifice of the hollow tube 51, and the other end orifice of the hollow tube 51 is detachably sleeved on the connecting rod 3.
The solid structure nail piece 52 structure is smooth, and fungus pieces are completely inoculated onto a new culture plate from the sampling sleeve 4 by utilizing the nail piece 52 structure after fungus piece sampling.
In an embodiment, when the connecting rod 3 is in the first position, the distance from the bottom of the adapter 6 to the bottom of the connecting rod 3 is greater than the length of the hollow tube 51, and the bottom of the connecting rod 3 does not protrude from the sampling sleeve 4; when the connecting rod 3 is in the second position, the bottom of the connecting rod 3 protrudes out of the sampling sleeve 4.
Specifically, the distance from the bottom of the adapter 6 to the bottom of the connecting rod 3 is greater than the length of the hollow tube 51, which is favorable for completely assembling or sleeving the hollow tube 51 on the connecting rod 3 to avoid the falling off of the proofing nails 5 in the use process; the bottom of the connecting rod 3 does not protrude out of the sampling sleeve 4, so that the sampling sleeve has enough sampling accommodating space, and a complete fungus block is obtained; when the connecting rod 3 is in the second position, the first spring 2 is compressed, and the bottom of the connecting rod 3 protrudes out of the sampling sleeve 4, so that the surface of the culture plate after sampling is inoculated.
In an embodiment, the piston further comprises a piston 7 and a first pressing block 8, a through hole is formed in the center of the piston 7, the top of the connecting rod 3 is connected with the first pressing block 8, the connecting rod 3 penetrates through the through hole and is fixedly connected with the piston 7, and the first spring 2 is abutted to the piston 7.
Specifically, when inoculating after sampling, the sampling sleeve 4 is vertically contacted with the surface of the culture plate and the bacteria block can be beaten out of the sampling sleeve 4 by the piston 7 and the first pressing block 8 to be inoculated on the culture plate.
In an embodiment, the removing device 9 further includes an outer cylinder 12 and a second spring 11, the outer cylinder 12 is sleeved on the outer circumference of the inner cylinder 1 in an axially displaceable manner, the bottom of the outer cylinder 12 abuts against the sampling sleeve 4, and when the outer cylinder 12 moves down under external force, the outer cylinder 12 drives the sampling sleeve 4 to be removed from the adapter 6; when the stress of the outer cylinder 12 disappears, the second spring 11 drives the outer cylinder 12 to return.
In an embodiment, the novel push rod further comprises a push rod 10, a clamping ring 13 and a handle 14, wherein a second pressing block 131 is arranged on the clamping ring 13, the second pressing block 131 is in butt joint with the top of the push rod 10, the bottom of the push rod 10 is in butt joint with the second spring 11, a supporting part is arranged on the outer wall of the inner cylinder 1, the bottom of the second spring 11 is in butt joint with the supporting part, the clamping ring 13 is arranged on the top of the outer cylinder 12 in a surrounding mode, and the handle 14 is connected with one end of the clamping ring 13.
Specifically, the second pressing block 131 is pressed to compress the second spring 11, so that the sampling sleeve 4 is automatically removed, and the purposes of disassembly, cleaning, disinfection and sterilization are achieved.
In one embodiment, the bottom of the outer cylinder 12 is conical and the handle 14 is semi-circular.
In one embodiment, the inner barrel 1, adapter 6 and sampling sleeve 4 are coaxially disposed.
The inner cylinder 1, the adapter 6 and the sampling sleeve 4 are coaxially arranged, namely the central axes of the inner cylinder 1, the adapter 6 and the sampling sleeve 4 are on the same straight line, and the inner cylinder 1, the adapter 6 and the sampling sleeve 4 are matched, so that sampling and inoculation of fungus blocks after sampling are facilitated; specifically, the sampling and inoculation process using the device is that the sampling sleeve 4 is perpendicular to the culture plate, the handle 14 and the outer barrel 12 are tightly held, the sampling sleeve 4 is inserted into the solid culture substrate for sampling, then the sampling device is vertically arranged on the upper surface of the culture plate, the sampling sleeve 4 is in contact with the surface of the solid culture medium, the first pressing block 8 is pressed, the sampling device is arranged, the fungus blocks are separated from the sampling sleeve 4 and attached to the surface of the solid culture medium, after inoculation is completed, the removing device 9 is pressed, the sampling sleeve 4 is automatically separated, and the sampling nails 5 are manually removed, so that the sampling and inoculation process is completed.
The foregoing description of the preferred embodiments of the utility model is not intended to be limiting, but rather is intended to cover all modifications, equivalents, and alternatives falling within the spirit and principles of the utility model.
Claims (10)
1. The solid fungus block sampling device is characterized by comprising an inner cylinder, a first spring, a connecting rod, a sampling sleeve and a sampling nail, wherein the first spring is arranged in the inner cylinder, the first spring is connected with the connecting rod, the connecting rod stretches into and passes through the bottom of the inner cylinder from the top of the inner cylinder, the connecting rod can move back and forth between a first position and a second position, and when the connecting rod is forced to move to the second position, the first spring is compressed; when the stress of the connecting rod disappears, the first spring is restored and drives the connecting rod to return to the first position; the bottom of connecting rod with the nail can dismantle the connection, the inner tube bottom is provided with the adapter, sampling sleeve can dismantle connect in on the adapter, by the connecting rod drives the nail of drawing a design wears out sampling sleeve or holding in the sampling sleeve.
2. The solid block sampling device of claim 1, wherein the sampling sleeve comprises a large sleeve and a small sleeve, the large sleeve and the small sleeve are coaxially arranged and connected with each other, the inner diameter of the large sleeve is larger than the inner diameter of the small sleeve, and the large sleeve is sleeved with the adapter sleeve.
3. The solid block sampling device according to claim 2, wherein the thickness of the small sleeve wall is 0.6-1.0 mm.
4. The solid fungus block sampling device according to claim 1, wherein the sample drawing nail comprises a hollow tube and a nail sheet, the nail sheet is of a solid structure, the nail sheet covers one end tube orifice of the hollow tube, and the other end tube orifice of the hollow tube is detachably sleeved on the connecting rod.
5. The solid block sampling device of claim 4, wherein when the connecting rod is in the first position, the distance from the bottom of the adapter to the bottom of the connecting rod is greater than the length of the hollow tube, and the bottom of the connecting rod does not protrude out of the sampling sleeve; when the connecting rod is in the second position, the bottom of the connecting rod protrudes out of the sampling sleeve.
6. The solid fungus block sampling device according to claim 1, further comprising a piston and a first pressing block, wherein a through hole is formed in the center of the piston, the first pressing block is connected to the top of the connecting rod, the connecting rod penetrates through the through hole and is fixedly connected with the piston, and the first spring is abutted to the piston.
7. The solid fungus block sampling device according to claim 1, further comprising a removal device, wherein the removal device comprises an outer cylinder and a second spring, the outer cylinder is sleeved on the periphery of the inner cylinder in an axial displacement manner, the bottom of the outer cylinder abuts against the sampling sleeve, and when the outer cylinder moves downwards under external force, the outer cylinder drives the sampling sleeve to be removed from the adapter; when the stress of the outer cylinder disappears, the second spring drives the outer cylinder to return.
8. The solid fungus block sampling device according to claim 7, further comprising a push rod, a clamping ring and a handle, wherein a second pressing block is arranged on the clamping ring, the second pressing block is in butt joint with the top of the push rod, the bottom of the push rod is in butt joint with the second spring, the outer wall of the inner cylinder is provided with a supporting part, the bottom of the second spring is in butt joint with the supporting part, the clamping ring is arranged on the top of the outer cylinder in a surrounding mode, and the handle is connected with one end of the clamping ring.
9. The solid block sampling device of claim 8, wherein the bottom of the outer cylinder is conical-like, and the handle is semicircular.
10. The solid block sampling device of claim 1, wherein the inner barrel, adapter and sampling sleeve are coaxially disposed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321560807.1U CN220116540U (en) | 2023-06-16 | 2023-06-16 | Solid fungus piece sampling device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321560807.1U CN220116540U (en) | 2023-06-16 | 2023-06-16 | Solid fungus piece sampling device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220116540U true CN220116540U (en) | 2023-12-01 |
Family
ID=88893074
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321560807.1U Active CN220116540U (en) | 2023-06-16 | 2023-06-16 | Solid fungus piece sampling device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220116540U (en) |
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2023
- 2023-06-16 CN CN202321560807.1U patent/CN220116540U/en active Active
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