CN206828533U - A kind of single bacterium colony picking device - Google Patents

A kind of single bacterium colony picking device Download PDF

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Publication number
CN206828533U
CN206828533U CN201720719550.8U CN201720719550U CN206828533U CN 206828533 U CN206828533 U CN 206828533U CN 201720719550 U CN201720719550 U CN 201720719550U CN 206828533 U CN206828533 U CN 206828533U
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CN
China
Prior art keywords
housing
core
core print
bamboo stick
bacterium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201720719550.8U
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Chinese (zh)
Inventor
裴杰
褚敏
郭宪
包鹏甲
梁春年
阎萍
冯瑞林
丁学智
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lanzhou Institute of Animal Husbandry and Veterinary Medicine CAAS
Original Assignee
Lanzhou Institute of Animal Husbandry and Veterinary Medicine CAAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
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Application filed by Lanzhou Institute of Animal Husbandry and Veterinary Medicine CAAS filed Critical Lanzhou Institute of Animal Husbandry and Veterinary Medicine CAAS
Priority to CN201720719550.8U priority Critical patent/CN206828533U/en
Application granted granted Critical
Publication of CN206828533U publication Critical patent/CN206828533U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

The utility model discloses a kind of single bacterium colony picking device, including choosing bacterium device, the bacterium device of choosing includes housing and inner core, the inner core runs through the housing by telescoping mechanism, the inner core lower end includes core print, the core print is made up of core print valve, the core print valve is opening shape under nature, when the core print is located in housing, core print is in closed form under the squeezing action of housing lower end, the core print stretches out housing in the presence of telescoping mechanism, core print valve, which opens, bites required bamboo stick, then returned in the presence of telescoping mechanism in housing, the bamboo stick carrys out picking colony in the presence of bacterium device is chosen.The utility model is easy to use, simple in construction, by by translucent cover, choose bacterium device, the bamboo stick connection that cooperates is set, solve the easily contaminated problem of culture medium during bacterium of choosing well;What is set chooses bacterium device and can firmly clamp bamboo stick, will not be dropped during bacterium is chosen, make experiment become it is simple easily.

Description

A kind of single bacterium colony picking device
Technical field
The utility model microorganism belonging to genus culture technique field, and in particular to one kind is used for the micro- life of picking on solid medium The single bacterium colony picking device of thing bacterium colony.
Background technology
Microbionation, culture and detection can be used on the experimental study of biology, during this, monoclonal bacterium colony Picking and Liquid Culture are most basic operations.It is thin by one because what a bacterium colony monoclonal represented is same gene type Caused by bacterium constantly breeding.It is all with requiring picking in the operation of bacterium in actication of culture and preservation, epidemiological study etc. Monoclonal bacterium colony carries out Liquid Culture, preservation or other subsequent operations.
In the operation of monoclonal bacterium colony picking, generally first the lid of solid culture ware is removed, then one with sterilizing Reason takes the photograph sub-folder and lives the toothpick to sterilize, or suction pipette head, another hand pick up culture dish, with toothpick on solid medium Blunt nosed or suction nozzle tip picking monoclonal bacterium colony.Then the toothpick for choosing monoclonal bacterium colony or pipettor gun head are directly placed into Fluid nutrient medium in test tube is enlarged culture.Follow-up bacterium operation and processing are carried out after having cultivated.
It can be seen that following several respects be present from operation above program:Remove the culture dish of lid, culture medium is straight Exposure is connect easily to pollute;When with taking the photograph sub-folder and living toothpick/suction nozzle, toothpick/suction nozzle easily comes off from taking the photograph on son, makes to test Journey becomes cumbersome and difficult;The hand-held hand for taking the photograph son is easily shaken, and is not easy picking single bacterium colony.
Utility model content
In view of the shortcomings of the prior art, the utility model purpose is that pollution, toothpick can be avoided by providing one kind Single bacterium colony picking device that is difficult for drop-off and accurately choosing bacterium.
To achieve these goals, the utility model uses following technical scheme:A kind of single bacterium colony picking device, including choose Bacterium device, the bacterium device of choosing include housing and inner core, and the inner core runs through the housing, the inner core lower end bag by telescoping mechanism Core print is included, the core print is made up of core print valve, and the core print valve is opens shape under nature, when the core print is located in housing When, core print is in closed form under the squeezing action of housing lower end, and the core print stretches out housing, core print valve in the presence of telescoping mechanism Open and bite required bamboo stick, then returned in the presence of telescoping mechanism in housing, the bamboo stick is choosing the effect of bacterium device Get off picking colony.
Preferably, the housing includes upper shell and lower house, and the housing upper end is provided with upper shed, and lower end, which is provided with down, opens Mouthful, and the junction of the upper shell and lower house is provided with snap ring.
Preferably, the inner core through housing and passes through snap ring, and the inner core upper end is stretched out from the upper shed, institute's rheme Spring is provided with the inner core cover in upper shell, and is provided with a spring dogging shoulder, the spring dogging shoulder is connected to inside upper shell Top.
Preferably, the core print valve forms preceding core bore and rear core bore in closure state, and the preceding core bore diameter is less than bamboo Diameter is signed, the rear core bore diameter is more than bamboo stick diameter.
Preferably, in addition to translucent cover, the culture dish for override open state.
Preferably, the translucent cover includes left sideshield and right sideshield, and the left sideshield and right sideshield are rotated by hinge Connection, and to be provided with a cover suitable for reading on the translucent cover top, bottom is the lower mouth of cover, and mouth is provided with arc card article under the cover, use In clamping culture dish.
The utility model beneficial effect is:The utility model is easy to use, simple in construction, by by translucent cover, choose bacterium device, The bamboo stick connection that cooperates is set, and solves the easily contaminated problem of culture medium during bacterium of choosing well;What is set chooses bacterium device Bamboo stick can be firmly clamped, will not be dropped during bacterium is chosen, experiment is become simple easy;Choosing bacterium device can rely on The top round mouth of bright cover can be held well chooses bacterium device to single bacterium colony progress picking, prevents the shake of hand from causing to choose the inaccurate of bacterium Really.
Brief description of the drawings
, below will be to embodiment in order to illustrate more clearly of the utility model embodiment or technical scheme of the prior art Or the required accompanying drawing used is briefly described in description of the prior art, it should be apparent that, drawings in the following description are only It is some embodiments of the utility model, for those of ordinary skill in the art, is not paying the premise of creative work Under, other accompanying drawings can also be obtained according to these accompanying drawings.
Fig. 1 is the structural representation of the utility model translucent cover;
Fig. 2 is the structural representation of the utility model translucent cover;
Fig. 3 is that the utility model chooses a kind of structural representation of embodiment of bacterium device;
Fig. 4 is the structural representation that the utility model chooses core print in bacterium device;
Fig. 5 is the structural representation of the utility model bamboo stick bucket;
Fig. 6 is the structural representation of the utility model bamboo stick bucket bung;
In figure:1 translucent cover, 2 choose bacterium device, 3 bamboo sticks, 4 bamboo stick buckets, 11 left sideshields, 12 right sideshields, 13 hinges, 14 cover it is suitable for reading, Core bore, 212 Spring Cards before core bore, 2113 behind the lower mouths of 15 covers, 16 arc card articles, 21 inner cores, 211 core prints, 2111 core print valves, 2112 Shoulder, 213 buttons, 22 springs, 23 housings, 231 upper shells, 232 lower houses, 2311 upper sheds, 2321 under sheds, 2322 snap rings, 41 stavings, 42 bungs.
Embodiment
The utility model is described further in conjunction with the accompanying drawings and embodiments.
A kind of single bacterium colony picking device, including bacterium device is chosen, the bacterium device of choosing includes housing and inner core, and the inner core is by stretching Contracting mechanism runs through the housing, and the inner core lower end includes core print, and the core print is made up of core print valve, the core under nature Head valve is opens shape, and when the core print is located in housing, core print is in closed form under the squeezing action of housing lower end, the core print Housing is stretched out in the presence of telescoping mechanism, core print valve, which opens, bites required bamboo stick, then in the presence of telescoping mechanism Return in housing, the bamboo stick carrys out picking colony in the presence of bacterium device is chosen.
The housing includes upper shell and lower house, and the housing upper end is provided with upper shed, and lower end is provided with shed, and institute The junction for stating upper shell and lower house is provided with snap ring.
Preferably, the inner core through housing and passes through snap ring, and the inner core upper end is stretched out from the upper shed, institute's rheme Spring is provided with the inner core cover in upper shell, and is provided with a spring dogging shoulder, the spring dogging shoulder is connected to inside upper shell Top.
As shown in figures 1 to 6, a kind of single bacterium colony picking device, including translucent cover, bacterium device and bamboo stick bucket are chosen, wherein, it is described It is bright cover 1 point be 12 two parts of left sideshield 11 and right sideshield, both are connected by the hinge 13 of top, both combinations after it is upper, Circular enclosure suitable for reading 14 and the lower mouth 15 of cover are respectively formed beneath, the bottom of translucent cover 1 is provided with arc card article 16, arc card article 16 For being connected to culture dish;The bacterium device 2 of choosing is internally provided with inner core 21, and the front end of inner core 21 is provided with core print 211, institute State core print 211 to be made up of three identical core print valves 2111, preceding core bore 2113 and rear core bore are provided with the core print 211 2112, the tail end of inner core 21 is provided with spring dogging shoulder 212 and button 213;The bacterium device 2 of choosing is internally provided with spring 22;Institute State to choose and housing 23 is provided with outside bacterium device 2, the subpackage of housing 23 contains upper shell 231 and lower house 232, and both pass through taenidium Button connects, and has the upper shed 2311 of circle on the tail end of upper shell 231, has on the tail end of lower house 232 and opened under circle Mouthfuls 2321, snap ring 2322 is provided with the tail end of lower house 232;Bamboo stick 3 is big end down column construction;Bamboo stick bucket 4 wraps Staving 41 and bung 42 are included, it is interior to contain bamboo stick 3.
Wherein, the left sideshield 11 and right sideshield 12 in closed state just with culture dish wall contacts, on the left side Culture dish can be firmly picked up under the collective effect of edge cover 11, right sideshield 12 and arc card article 16.
The core print 211 is the preferable steel material of elasticity, and the core print 211 is in open state in its natural state, core Head valve 2111 opens, and is in closure state in the case where the under shed 2321 of the front end of lower casing 232 constrains.The core print 211 is being opened Put its preceding core bore 2113 under state and may be inserted into bamboo stick 3, bamboo stick 3 can be picked up in closed state;The preceding core bore 2113 is straight Footpath is less than the diameter of bamboo stick 3, and the rear diameter of core bore 2112 is more than the diameter of bamboo stick 3, can select to use the bamboo according to bacterium colony size The major part of label 3 or microcephaly carry out picking colony.
In experimentation, most preferred embodiment can be completed by following steps:
1. pair translucent cover 1, choose bacterium device 2, bamboo stick 3 and bamboo stick bucket 4, using 121 DEG C, the sterilizing methods of 20 minutes.
2. by above-mentioned translucent cover 1, choose bacterium device 2, bamboo stick 3, bamboo stick bucket 4 and be intended to picking colony culture dish be put in it is super In net workbench.
Covered 3. opening on culture dish, upper lid is set aside, the left sideshield 11 and right sideshield 12 of translucent cover 1 is opened, will train Support ware and be put in from lower mouth is covered among translucent cover 1, close left sideshield 11 and right sideshield 12, culture dish is firmly clipped in when a little Among bright cover 1.
4. bacterium device 2 is chosen hand-held, presses and chooses bacterium device button 213, bamboo stick is encased with the core print 211 of opening, release button 213, Now core print 211 can clamp bamboo stick 3.
5. a hand picks up the interior translucent cover 1 for having culture dish, another is hand-held to choose bacterium device 2 with bamboo stick 3, will choose bacterium Device 2 stretches into translucent cover 1 from covering suitable for reading 14, gently dips the single bacterium colony for needing to be inoculated with culture medium with bamboo stick 3.
6. bacterium device 2 is chosen in taking-up, bamboo stick 3 is directed at the interior blake bottle bottleneck for having nutrient solution, presses the button for choosing bacterium device 2 213, the bamboo stick 3 with bacterium colony will be fallen into nutrient solution
7. bacterium is cultivated with suitable condition.
The standardized element that the utility model uses can commercially, shaped piece according to specification and accompanying drawing Record can carry out customized, the specific connected mode of each part is using bolt ripe in the prior art, rivet, weldering The conventional meanses such as connect, machinery, part and equipment are using in the prior art, conventional model, plus circuit connection using existing Conventional connected mode, will not be described in detail herein in technology.
In this description, the utility model is described with reference to its specific embodiment.But it is clear that still can be with Various modifications and alterations are made without departing from spirit and scope of the present utility model.Therefore, specification and drawings are considered as It is illustrative and not restrictive.

Claims (6)

1. a kind of single bacterium colony picking device, it is characterised in that including choosing bacterium device, the bacterium device of choosing includes housing and inner core, described Inner core runs through the housing by telescoping mechanism, and the inner core lower end includes core print, and the core print is made up of core print valve, natural shape The core print valve is opens shape under state, and when the core print is located in housing, core print is under the squeezing action of housing lower end in closure Shape, the core print stretch out housing in the presence of telescoping mechanism, and core print valve, which opens, bites required bamboo stick, then in telescopic machine Returned in the presence of structure in housing, the bamboo stick carrys out picking colony in the presence of bacterium device is chosen.
2. single bacterium colony picking device according to claim 1, it is characterised in that the housing includes upper shell and lower casing Body, the housing upper end are provided with upper shed, and lower end is provided with shed, and the junction of the upper shell and lower house is provided with card Ring.
3. single bacterium colony picking device according to claim 2, it is characterised in that the inner core is through housing and passes through card Ring, the inner core upper end are stretched out from the upper shed, and the inner core cover in upper shell is provided with spring, and is provided with a bullet Spring dogging shoulder, the spring dogging shoulder are connected to the top inside upper shell.
4. the single bacterium colony picking device according to claim 1-3 any one, it is characterised in that the core print valve is closing Preceding core bore and rear core bore are formed during state, the preceding core bore diameter is less than bamboo stick diameter, and it is straight that the rear core bore diameter is more than bamboo stick Footpath.
5. the single bacterium colony picking device according to claim 1-3 any one, it is characterised in that also including translucent cover, use In the culture dish of override open state.
6. single bacterium colony picking device according to claim 5, it is characterised in that the translucent cover includes left sideshield and the right Cover, and the left sideshield and right sideshield are rotatablely connected by hinge, and the translucent cover top is provided with that a cover is suitable for reading, and bottom is The lower mouth of cover, and mouth is provided with arc card article under the cover, for clamping culture dish.
CN201720719550.8U 2017-06-20 2017-06-20 A kind of single bacterium colony picking device Expired - Fee Related CN206828533U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201720719550.8U CN206828533U (en) 2017-06-20 2017-06-20 A kind of single bacterium colony picking device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201720719550.8U CN206828533U (en) 2017-06-20 2017-06-20 A kind of single bacterium colony picking device

Publications (1)

Publication Number Publication Date
CN206828533U true CN206828533U (en) 2018-01-02

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201720719550.8U Expired - Fee Related CN206828533U (en) 2017-06-20 2017-06-20 A kind of single bacterium colony picking device

Country Status (1)

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CN (1) CN206828533U (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112126586A (en) * 2020-09-29 2020-12-25 安徽省金裕生物科技开发有限公司 Bacterial colony picking device for wine making and use method thereof
CN113201446A (en) * 2021-05-10 2021-08-03 北京化工大学 Automatic bacterial colony of high flux picks device

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112126586A (en) * 2020-09-29 2020-12-25 安徽省金裕生物科技开发有限公司 Bacterial colony picking device for wine making and use method thereof
CN112126586B (en) * 2020-09-29 2023-08-18 安徽省金裕生物科技开发有限公司 Colony picking device for brewing and application method thereof
CN113201446A (en) * 2021-05-10 2021-08-03 北京化工大学 Automatic bacterial colony of high flux picks device

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CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20180102

Termination date: 20180620