A kind of microorganism culturing based devices
Technical field
The present invention relates to a kind of microorganism culturing based devices.
Background technology
Microorganism culturing based devices is widely used in colony inoculation, cultivation, separation, detection, and it is the important work of of the aspects such as health care, inspection and quarantine, Disease epizootic, food safety that micro-biological samples is cultivated.Current microbial inoculant, cultivation, separation, detection are cultivated microorganism culturing based devices used and are comprised: a sampling ring and a glass culture dish.The working method of described microorganism culturing based devices is:
(1) in glass culture dish, operator's hand-held sampling ring carries out artificial sterilizing;
(2) a small amount of micro-biological samples is annotated the surface of the nutrient solution in glass culture dish by sampling ring gently that be moistened with microorganism;
(3) diluted by sectional streak in media surface by the different cells in microorganism mixed in together or same microbial population by the method for line and obtain the individual cells of more independent distribution, after cultivating, growth and breeding becomes single bacterium colony.
Obviously, existing microorganism culturing based devices has following shortcoming:
(1) when operating, testing staff is according to the sensation of hand, the end of transfering loop is made just to touch the surface of nutrient solution, then cultivate at a certain temperature, after bacterial growth is large, then observe with proofing unit, detect colony number wherein, do not have stability, the arbitrariness of line is higher;
(2) due to adopt manual, make transfering loop just touch the surface of nutrient solution with feeling, time and contact the surface of nutrient solution, time and the phenomenon left is difficult to avoid, easy cross infection or bacteria distribution poor effect;
(3) owing to adopting artificial hatched manner, cause result can not stdn, experimental result recognizes difficulty again, judges inaccurate;
(4) manual operation is wasted time and energy, inefficiency;
(5) have potential biological pollution and hazardness, operator easily come to harm in open experimental situation.
Generally speaking, the microorganism culturing based devices of prior art also exist that biological safety is low, operation can not stdn, manual operation is random large, human cost is high and inefficiency, experimental result recognize the low problem of rate again.
Summary of the invention
In order to solve, the biological safety that prior art microorganism culturing based devices exists is low, operation can not stdn, manual operation random large, human cost high-level efficiency is low, result recognizes the problems such as rate is low again, the present invention proposes a kind of microorganism culturing based devices.
Microorganism culturing based devices of the present invention, comprise substratum and scriber, described substratum comprises box body, lid, spring, buckle and deck, described box body and lid with the use of and chain connection, namely in hinged hinged place mounting spring, when described spring makes lid open, state is held open; Described box body is also provided with deck, described lid is also provided with buckle, described buckle has latch, described buckle is fastened by latch and deck, is kept closed when lid is closed; Described spring is torsion spring or top spring; Described scriber comprises handle, crossbeam and transfering loop, the top of described handle is inboardend, and be detachably connected with the mechanical mobile device of exterior mechanical auto-plant, the other end of described handle is fixedly connected with described crossbeam, described crossbeam is fixedly installed at least one transfering loop.
Further, described spring is torsion spring or top spring;
Further, the position of described buckle and deck can exchange;
Further, described box body and with it with the use of the profile of lid can be: circular, square, prismatic or ellipse;
Further, described transfering loop is 1 to 8;
Further, described transfering loop is ring-type or spherical.
The Advantageous Effects of microorganism culturing based devices of the present invention is: biosafety issues is solved; Protection environment and operator, operational standardization, human cost reduces; working efficiency increases, operating process standardized mechanical, and experimental result recognizes rate again to be increased.
Accompanying drawing explanation
Accompanying drawing 1 is the structural representation of substratum of the present invention;
Accompanying drawing 2 is the closedown shape body schematic diagram of substratum of the present invention;
Accompanying drawing 3 is the unlatching shape body schematic diagram of substratum of the present invention;
Accompanying drawing 4 is a kind of embodiment schematic diagram of substratum of the present invention;
Accompanying drawing 5 is the structural representation of scriber of the present invention;
Accompanying drawing 6 is the structural representation of microorganism culturing based devices of the present invention;
Below in conjunction with the drawings and specific embodiments, microorganism culturing based devices of the present invention is further described.
Embodiment
Accompanying drawing 1 is the structural representation of microorganism culturing based devices of the present invention, accompanying drawing 2 is the closedown shape body schematic diagram of microorganism culturing based devices of the present invention, accompanying drawing 3 is the unlatching shape body schematic diagram of microorganism culturing based devices of the present invention, accompanying drawing 4 is a kind of embodiment schematic diagram of microorganism culturing based devices of the present invention, accompanying drawing 5 is the structural representation of scriber of the present invention, accompanying drawing 6 is the structural representation of microorganism culturing based devices of the present invention, in figure, 1 is substratum, 2 is scriber, 1.1 is box body, 1.2 is lid, 1.3 is spring, 1.4 is buckle, 1.5 is deck, 2.1 is handle, 2.2 is crossbeam, 2.3 is transfering loop.As shown in the figure, microorganism culturing based devices of the present invention, comprise substratum 1 and scriber 2, described substratum 1 comprises box body 1.1, lid 1.2, spring 1.3, buckle 1.4 and deck 1.5, described box body 1.1 and lid 1.2 with the use of and chain connection, namely in hinged hinged place mounting spring 1.3, when described spring 1.3 makes lid 1.2 open, state is held open; Described box body 1.1 is also provided with deck 1.5, described lid 1.2 is also provided with buckle 1.4, described buckle 1.4 has latch, described buckle 1.4 is fastened by latch and deck 1.5, is kept closed when lid 1.2 is closed; Described spring 1.3 is torsion spring or top spring; Described scriber 2 comprises handle 2.1, crossbeam 2.2 and transfering loop 2.3, the top of described handle 2.1 is inboardend, and be detachably connected with the mechanical mobile device of exterior mechanical auto-plant, the other end of described handle 2.2 is fixedly connected with described crossbeam 2.2, described crossbeam 2.2 is fixedly installed at least one transfering loop 2.3.
Play opening/closing lid 1.2 and the effect holding on/off shape body to better reach, spring 1.3 of the present invention is torsion spring or top spring; In the specific implementation, in order to the requirement of the existing exterior mechanical auto-plant of more convenient cooperation, the position of described buckle 1.4 and deck 1.5 can exchange; Same, it will be appreciated by persons skilled in the art that the outer shape of substratum 1 does not affect implementation result of the present invention, therefore, described box body 1.1 and with it with the use of the profile of lid 1.2 can be: circular, square, prismatic or ellipse; In order to better increase work efficiency, described transfering loop 2.3 is 1 to 8; In order to play effect of better ruling, described transfering loop 2.3 is ring-type or spherical.In the specific implementation, microorganism culturing based devices of the present invention can be that one to four cover operates simultaneously, for same microbiological specimens, can carry out many groups at one time and detects and judge, increase working effect.
When concrete operations microorganism culturing based devices of the present invention, its step is as follows:
(1) deck 1.5 that the box body 1.1 of exterior mechanical bar touching substratum 1 is arranged makes buckle 1.4 unclamp, lid 1.2 by spring 1.3 and box body 1.1 in keeping this opened condition after opening;
(2) open exterior mechanical auto-plant, its mechanical mobile device drives the handle 2.1 of scriber 2 of the present invention to move according to the program arranged, and meanwhile, substratum 1 is positioned at the below of scriber 2 by transport unit;
(3) scriber 2 samples, and namely mechanical mobile device band fixed handle 2.1 dips micro-biological samples by described transfering loop 2.3, and rules on microorganism culturing based devices;
(4), after line, scriber 2 leaves;
(5) close substratum 1, the exterior mechanical bar being namely positioned at top touches lid 1.2, and the deck 1.5 that the buckle of lid 1.2 1.4 and box body 1.1 are arranged fastens, and is kept closed when substratum 1 is closed;
(6) scriber 2 that used of mechanical mobile device automatic dismantling, and obtain another new scriber 2;
(7) substratum 1 having completed line is left by transport unit, and next new substratum 1 is positioned at the below of scriber 2 by transport unit;
(8) above-mentioned steps () is repeated to (seven);
(9) described step () and step (two) can be exchanged.
In the specific implementation, the travel direction of scriber, according to shown in accompanying drawing 6, moves to B by A, then moves to C, then move to D, thus complete above-mentioned scratching process.
Obviously; the advantage that microorganism culturing based devices of the present invention has low price, cooperation thought by easy to operate, not fragile, easy and multiple unlatching exterior mechanical auto-plant; simultaneously; the working method of microorganism culturing based devices of the present invention makes biosafety issues be solved; and Protection environment and operator, operational standardization, human cost reduces; working efficiency increases, operating process standardized mechanical, and experimental result recognizes rate again to be increased.