CN206418141U - A kind of Embryo Culture ware - Google Patents
A kind of Embryo Culture ware Download PDFInfo
- Publication number
- CN206418141U CN206418141U CN201720087281.8U CN201720087281U CN206418141U CN 206418141 U CN206418141 U CN 206418141U CN 201720087281 U CN201720087281 U CN 201720087281U CN 206418141 U CN206418141 U CN 206418141U
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- Prior art keywords
- culture
- embryo
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- region
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- 210000001161 Embryo, Mammalian Anatomy 0.000 title claims abstract description 57
- 239000004033 plastic Substances 0.000 abstract description 4
- 229920003023 plastic Polymers 0.000 abstract description 4
- 238000010276 construction Methods 0.000 abstract description 2
- 210000002257 embryonic structures Anatomy 0.000 description 14
- 210000004027 cells Anatomy 0.000 description 13
- 239000001963 growth media Substances 0.000 description 11
- 238000000338 in vitro Methods 0.000 description 7
- 208000000509 Infertility Diseases 0.000 description 5
- 239000005662 Paraffin oil Substances 0.000 description 5
- 230000036512 infertility Effects 0.000 description 5
- 231100000535 infertility Toxicity 0.000 description 5
- -1 Polypropylene Polymers 0.000 description 4
- 239000004743 Polypropylene Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 230000004720 fertilization Effects 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 229920001155 polypropylene Polymers 0.000 description 4
- 239000004425 Makrolon Substances 0.000 description 3
- 239000004793 Polystyrene Substances 0.000 description 3
- 230000002068 genetic Effects 0.000 description 3
- 229920000515 polycarbonate Polymers 0.000 description 3
- 229920002223 polystyrene Polymers 0.000 description 3
- 210000004681 Ovum Anatomy 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 2
- 150000001336 alkenes Chemical class 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 210000002459 Blastocyst Anatomy 0.000 description 1
- 206010061205 Hereditary disease Diseases 0.000 description 1
- 108009000403 Preimplantation Embryo Proteins 0.000 description 1
- 230000001464 adherent Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000005755 formation reaction Methods 0.000 description 1
- 230000002452 interceptive Effects 0.000 description 1
- 101700010476 lid-1 Proteins 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000001850 reproductive Effects 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 210000001519 tissues Anatomy 0.000 description 1
- 239000001993 wax Substances 0.000 description 1
Abstract
The utility model discloses a kind of Embryo Culture ware.The Embryo Culture ware includes ware lid, ware body and the culture region that equal biological safety level plastics are made, and the enclosing that culture region is not waited by height is divided into some culture cells, and has numeral and letter designation in culture edges of regions.Mutually isolated culture cell is formed in same culture dish, embryo is individually cultivated, normal or rocking for moderate strength can not make embryo leave respective culture cell, so that completely isolated between ensureing embryo, it is to avoid mutually obscure.Its is simple in construction, easy to use, with low cost, during the special IVF ET deriving technologies such as PGD are implemented, will significantly improve operating efficiency and reduces medical-risk.
Description
Technical field
The utility model belongs to Bioexperiment apparatus field, and in particular to a kind of Embryo Culture ware.
Background technology
According to the World Health Organization（WHO）The 10-15% couple at child-bearing age is perplexed by infertility in estimation, developed country.In development
In country（Including China）, the women of child-bearing age for having 1.86 hundred million to 2002 suffer from infertility.The statistics of 2014 shows,
The incidence of China's reproductive population infertility is 12.5%, and infertility number is more than 40,000,000.Vitro fertilization-embryo implanting（in-
vitro fertilization and embryo transfer, IVF-ET）Technology is the important hand of current treatment infertility
Section.From 1978 since the first " test-tube baby " in the world is born in Britain, current developed country helps pregnant birth by IVF-ET
Baby accounted for natus sum 1-3%, estimate whole world test-tube baby births more than 5,000,000 people.
Some IVF-ET deriving technology needs to carry out all embryos of patient individually to distinguish and mark, in order to each
The situation of embryo is analyzed and followed the trail of.For example, implementing preimplantation embryo genetic diagnosis（preimplantation
genetic diagnosis, PGD）, it is necessary to which the embryo all to patient individually cultivate and mark during technology,
Then the cell that biopsy is a small amount of from these embryos carries out genetic analysis, so that it is determined that whether these embryos suffer from heredity disease
Disease, only diagnosing normal embryo can be implanted into patient's body, and abnormal embryo can not be transplanted.Now, for training
The mutually isolated and mark of embryo is then very crucial during supporting, if embryo obscures during culture, having can
Abnormal embryo can be caused to be implanted into patient's body, offspring of the birth with hereditary disease.
Human embryonic in vitro culture is carried out in culture dish, and it is female that laboratories in vitro fertilization most of at present carry out ovum
The in vitro culture of cell and embryo are to use common Tissue Culture Dish（Such as 35mm, 60mm or 100mm Petri dish）.It is micro-
Drop culture is the most frequently used Embryo Culture method, and 10-50 microlitres of culture medium exactly is dropped in into culture dish bottom, is then covered above
Coping stone wax oil, embryo is placed in culture medium droplet and cultivated, human embryos are subglobulars and volume is very small, wherein
About 120-150 microns of human fertilization ovum and cleavage stage embryo diameter, about 200-300 microns of blastaea, each droplet can be put
Put 1-3 embryo.
Carrying out the patient of IVF-ET treatments often has multiple embryos to need to carry out in vitro culture, due to the form of each embryo
It is closer to, it is impossible to directly embryo is marked.If necessary to individually be distinguished for each embryo, the most frequently used side
Method is the droplet for preparing respective numbers according to the quantity of embryo in culture dish, and 1 embryo, Ran Hou are placed in each droplet
Reference numerals on each droplet.Because human embryos are close to spherical, and there is the protection of oolemma, embryo will not also occur
The same adherent fixed growth of ordinary cells, is very easy to be moved in culture medium.In addition, culture medium and paraffin oil are
Liquid, the drift for easily occurring droplet.Therefore, mobile and operation microtitre plate needs action is soft, and strength is slightly larger may also
Cause microdroplet dispersion, embryo's drift may be scattered in other droplets by scattered culture medium droplet, cause embryo mutually to obscure, or
Embryo is floated into paraffin oil meter face causes loss.
In addition, there is the culture dish of some commercializations at present（Such as WOW culture dishes) there can be some apertures single at ware bottom
Each embryo is solely placed, in order to enter line delay to the growth course of each embryo by microcam（timelapse）Into
Picture.But these apertures are all under same culture medium droplet, to be not mutually isolated between embryo, and aperture is shallower
Also smaller, slight rocking this may result in embryo and be floated out from aperture.For each droplet individually culture, more hold
Embryo is easily caused to obscure.
The method insured the most is to prepare a culture dish for each embryo and individually mark and cultivate, but is worked as
When patient's embryo's quantity is more（Such as 10-20 even more more）, then need substantial amounts of culture dish, this will dramatically increase cost,
Operating time and difficulty.
Therefore, how simply, it is safe and reliable to embryo carry out individually isolation cultivate and mark, prevent embryo it
Between mutually obscure, be the problem that most laboratories in vitro fertilization are not yet solved very well.This problem is solved, is implementing PGD etc.
During special IVF-ET deriving technologies, operating efficiency will be significantly improved and medical-risk is reduced.
Utility model content
The utility model is intended to overcome the deficiencies in the prior art there is provided a kind of culture dish, and the device can be in a culture
Individually isolation culture and mark are carried out to all embryos of patient in ware, it is to avoid mutually obscuring between embryo.
To achieve these goals, the technical scheme that the utility model is used is：
The Embryo Culture ware, including ware lid, ware body and culture region；The culture region is arranged on side bottom in ware body
Face, the culture region is divided into several culture cells by outside enclosing and middle enclosing.
Provided with numeral or alphabetic flag around the culture region.The height of the outside enclosing is higher than the height of middle enclosing
Degree.
The utility model is described in further detail below：
In the utility model, ware body bottom center is divided into the cultivation region of a groined type by a plurality of enclosing of certain altitude
Domain, culture includes multiple culture cells in region.
The culture outermost enclosing in region is significantly higher than the enclosing of centre.When preparing culture medium, culture medium is added and trained
Support in region, culture medium height is concordant with the height of middle enclosing, covering paraffin oil above culture medium, paraffin oil thickness is covered
Height with cultivation region outermost enclosing is concordant.Each cultivate small indoor full of culture medium, culture is ware bottom, surrounding below cell
It is enclosing, top is paraffin oil, each embryo is separately positioned at the small interior of culture, can be isolated completely with other embryos.In
Between the height of enclosing be 3-5 times of embryo's diameter, it is small that the rocking of normal or moderate strength can not be that embryo leaves respective culture
Room, so that completely isolated between ensureing embryo, it is to avoid mutually obscure.
Letter and number is carved with the culture dish bottom in the laterally and longitudinally outside of grid respectively, passes through the group of letter and number
Closing can be marked and distinguish to each small indoor embryo of culture, such as A1 embryos, B1 embryos ....
All grids, numeral and letter, culture dish bottom is integrally formed by identical material and is made.
The environment of Embryo Culture is substantially similar with original independent droplet culture, therefore, and the development for not interfering with embryo is dived
Energy.
According to embodiment of the present utility model, further optimization can also be made to the utility model, below for optimization after
The technical scheme of formation：
Preferably, described Embryo Culture ware and conventional Petri dish（Such as 35mm, 60mm or 100 culture dishes）
Profile is essentially identical, and simply ware body inside bottom surface is provided with the cultivation region that a groined type is divided into by a plurality of enclosing of certain altitude
Domain.
Preferably, the Embryo Culture ware is identical with conventional Petri dish material, is various types of plastics, such as
Polypropylene, polystyrene or makrolon, preferably polypropylene.
The size of culture cell can be customized as needed with quantity in the size of culture dish, and culture region.
The 60mm culture dishes commonly used with laboratory（57mm×15mm）Exemplified by：The a height of 10mm of ware lid, external diameter is 57mm, and thickness is 1mm, interior
Footpath is 55mm；The a height of 14mm of ware body, external diameter is 53mm, and enclosing thickness internal diameter in ware body edge is 1mm, and ware base thickness degree is 4mm.Embodiment
Using 5 × 5 groined type culture region, culture region length and width are 25 ± 5mm, and the culture outermost enclosing in region is highly
4-6mm, middle enclosing is highly 2-3mm, and enclosing thickness is 1mm, is divided into 25 culture cells, each cultivates small indoor length and width
It is 4 ± 1mm, 25 embryos of culture just at most can be individually isolated so in 1 culture dish.
The utility model is identical with conventional Petri dish material, is the plastics of biological safety level（Such as poly- third
Alkene, polystyrene or makrolon, preferably polypropylene）, profile also with conventional Petri dish substantially close to.Therefore, with
Cellar culture ware is compared, during the utility model is operated with vitro culture in vitro, will not bring extra harmful effect.
Compared with prior art, the beneficial effects of the utility model are：The utility model is formed in same culture dish
Mutually isolated culture cell, normal or rocking for moderate strength can not make embryo leave respective culture cell, so as to ensure
It is completely isolated between embryo, it is to avoid mutually to obscure.Its is simple in construction, easy to use, with low cost, and implementing, PGD etc. is special
During IVF-ET deriving technologies, operating efficiency will be significantly improved and medical-risk is reduced.
Brief description of the drawings
Fig. 1 is the utility model structural representation；
Fig. 2 is Fig. 1 A-A sectional views；
In figure：1st, ware lid；2nd, ware body；3rd, mark；4th, cell is cultivated；5th, middle enclosing；6th, outside enclosing；7th, cultivation region
Domain.
Embodiment
Referring to Fig. 1 and Fig. 2, the Embryo Culture ware includes ware lid 1, ware body 2 and culture region 7, it is characterised in that described
Culture region 7 is arranged on the inside bottom surface of ware body 2, and the culture region 7 is divided into several trainings by outside enclosing 6 and middle enclosing 5
Support cell 4.
Wherein, provided with numeral or alphabetic flag 3 around the culture region 7.The height of the outside enclosing 6 is higher than centre
The height of enclosing 5.
The culture dish by biological safety level plastics（Such as polypropylene, polystyrene or makrolon, preferably poly- third
Alkene）Process.All grids, numeral and letter, culture dish bottom is integrally formed by identical material and is made.Ware lid is a height of
10mm, external diameter is 57mm, and thickness is 1mm, and internal diameter is 55mm；The a height of 14mm of ware body, external diameter is 53mm, and ware body edge enclosing is thick interior
Footpath is 1mm, and ware base thickness degree is 4mm.Embodiment uses 5 × 5 groined type culture region 7, and culture region length and width are 25 ±
5mm, the height of enclosing 6 of culture areas outside is 4-6mm, and the middle height of enclosing 5 is 2-3mm, and enclosing thickness is 1mm, is divided into
25 culture cells 4, it is 4 ± 1mm each to cultivate small indoor length and width, so in 1 culture dish just can at most individually every
From 25 embryos of culture.Letter and number mark 3 is carved with the culture dish bottom in the laterally and longitudinally outside of grid respectively, passes through word
Female sum combinatorics on words can be marked and distinguish to each small indoor embryo of culture, such as A1 embryos, B1 embryos.
Claims (3)
1. a kind of Embryo Culture ware, including ware lid（1）, ware body（2）And culture region（7）, it is characterised in that the culture region
（7）It is arranged on ware body（2）Inside bottom surface, the culture region（7）By outside enclosing（6）With middle enclosing（5）It is divided into several
Cultivate cell（4）.
2. Embryo Culture ware as claimed in claim 1, it is characterised in that the culture region（7）Surrounding is provided with numeral or word
Female mark is remembered（3）.
3. Embryo Culture ware as claimed in claim 1, it is characterised in that the outside enclosing（6）Height enclosed higher than centre
Gear（5）Height.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720087281.8U CN206418141U (en) | 2017-01-23 | 2017-01-23 | A kind of Embryo Culture ware |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720087281.8U CN206418141U (en) | 2017-01-23 | 2017-01-23 | A kind of Embryo Culture ware |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206418141U true CN206418141U (en) | 2017-08-18 |
Family
ID=59570602
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720087281.8U Expired - Fee Related CN206418141U (en) | 2017-01-23 | 2017-01-23 | A kind of Embryo Culture ware |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206418141U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109486677A (en) * | 2018-12-11 | 2019-03-19 | 黑龙江省农业科学院畜牧研究所 | A kind of Embryo Culture position limiting fence |
-
2017
- 2017-01-23 CN CN201720087281.8U patent/CN206418141U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109486677A (en) * | 2018-12-11 | 2019-03-19 | 黑龙江省农业科学院畜牧研究所 | A kind of Embryo Culture position limiting fence |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170818 Termination date: 20200123 |