CN205603569U - Tray is transported to culture dish - Google Patents
Tray is transported to culture dish Download PDFInfo
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- CN205603569U CN205603569U CN201620020108.1U CN201620020108U CN205603569U CN 205603569 U CN205603569 U CN 205603569U CN 201620020108 U CN201620020108 U CN 201620020108U CN 205603569 U CN205603569 U CN 205603569U
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- culture dish
- incubator
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Abstract
The utility model discloses a tray is transported to culture dish. Tray is transported to culture dish is metal soleplate and the metal curb plate of surface through sanding treatment through sanding treatment including the surface, be equipped with a plurality of bleeder vents on metal soleplate and the metal curb plate. The utility model discloses a tray is transported to culture dish can make things convenient for once only transporting the culture dish of more quantity of safety to pass in and out the incubator, can guarantee interior heat of incubator and gaseous exchange simultaneously. Its simple structure, convenient to use, low cost can comparatively effectual improvement relapse the influence of switch box antithetical couplet written on doors incubator temperature and gas strength, and human gamete and embryonal growth latent energy are maintain to the at utmost. The utility model discloses except being applicable to to the transportation of human gamete and embryo culture ware in the laboratory in vitro fertilization, also be applicable to other laboratories such as cell biology, embryology, zoology and microbiology that need carry out cell, tissue, organ and microbial cultivation.
Description
Technical field
This utility model belongs to biotic experiment apparatus field, is specifically related to a kind of pallet be easy to and be transferred in incubator by culture dish in high volume.
Background technology
Estimating according to the World Health Organization (WHO), in developed country, the couple at child-bearing age of 10-15% is perplexed by infertility.In developing country (including China), the women of child-bearing age having 1.86 hundred million to 2002 suffer from infertility.The statistical data of 2014 shows, the incidence rate of China's reproductive population infertility is 12.5%, and infertility number is more than 40,000,000.Vitro fertilization-embryo implanting (in-vitro
Fertilization and embryo transfer, IVF-ET) technology is the important means treating infertility at present.From 1978 since Britain's birth is the first " test-tube baby " in the world, current developed country has accounted for the 1-3% of natus sum by the baby that ART gravidity assisting is born, and estimates that the births of whole world test-tube baby are more than 5,000,000 people.
In vitro during fertilization-embryo transfer technology implementation, need in vitro mankind's gamete (sperm and oocyte) and embryo to be carried out In vitro culture.Suitable temperature and pH value are to maintain gamete and the essential condition of embryo's normal existence, and the various normal physiological activities such as once temperature and the pH value of environment changes, its metabolism are by influenced to different extents.The temperature of optimum mankind's gamete and embryo cutting is about 37 DEG C, and optimum pH value is 7.3 ± 0.5.At present, most of external fertilization laboratorys all use conventional CO2Incubator (water-jacket typ or air-casing formula) carries out In vitro culture, and this incubator passes through electric heating equipment, and fan and temperature probe be normal temperature in maintaining incubator, passes through CO2Flow valve, fan and inductive probe guarantee CO2Concentration (5-6%) thus normal pH value in maintaining culture medium.
Switch incubator will cause temperature to decline, and due to CO2Lowering of concentration and the Medium's PH Value that causes rises.There are research display, No. 1 the conventional CO of switch2After incubator (water-jacket typ or air-casing formula) chamber door, temperature and CO2 concentration in case reach the most normal, take around 30 minutes；And continuous No. 3 switch-boxes are behind the door, then need could recover for more than 1 hour.It is highstrung that mankind's gamete and embryo are fluctuated for temperature and pH.Of short duration temperature declines (5-10 minute), may cause the depolymerization of spindle microtubule albumen, thus aneuploid or chimeric generation during causing the meiosis of oocyte or the mitosis of embryo.The temperature of long period declines (＞ 1 hour), it will change gamete and the metabolic process of embryo, increases chromosomal abnormality odds, and the potential that its continuation of irreversible reduction is grown.Owing to intracellular biochemical reaction is all to occur under the pH value of physiological status, the rising of pH value will cause intracellular metabolism to get muddled, alkalosis even occurs, cause gamete and embryonic death, even the slight of pH value rises (such as rising to 7.5 from 7.3), it is also possible to cause fetal development slow or fragment increases.Therefore during mankind's gamete and embryo are carried out In vitro culture, if at utmost maintaining the stability of vitro, reducing the external environment fluctuation impact on fetal development, being the major issue that each external fertilization laboratory must take at present.And switch chamber door repeatedly, by causing the vitro culture conditions in incubator to deviate significantly from normal range, have a strong impact on the normal development of gamete and embryo.
The incubator Limited Number of current most of external fertilization laboratory, needs in each incubator to place ten several even dozens of culture dishs.Therefore, in daily work, it is often necessary to taken out in incubator by multiple culture dishs and carry out manipulation in vitro and observation, continue to cultivate in then placing into incubator.Additionally every day is after having prepared microdroplet culture medium, it is also desirable to substantial amounts of culture dish is put into incubator and is balanced.If a culture dish turnover incubator can only be transported every time, then need repeated multiple times unlatching incubator；If multiple culture dish mounted on top are transported, then it is susceptible to landing, and causes serious consequence.
Part external fertilization laboratory uses medical stainless steel pallet, disposably together with pallet, more culture dish is put into incubator.But, most of water-jacket typs and air-casing formula CO2Incubator is circulated by fans drive gas, reaches to cultivate the temperature inside the box and CO2The balance of concentration, the dividing plate of the culture dish holding within incubator has many air-vents, to help circulating of incubator internal gas.Common medical stainless steel pallet will stop the air-vent on incubator dividing plate, hinders the circulation of incubator internal gas, causes temperature and the CO of zones of different in incubator2Concentration there are differences (too high or too low), thus affects stablizing of culture medium temperature and pH value, and affects the potentiality of development of gamete and embryo further.
Therefore, how can be in the case of incubator limited amount, the needs of observation in vitro and operation can be disclosure satisfy that, the number of times of switch incubator can be reduced again to greatest extent, the transhipment culture dish of safe ready, do not affect temperature gas exchange incubator within simultaneously, be the difficult problem that solves the most very well of majority external fertilization laboratory.Solve this difficult problem by can the most effectively improve a large amount of mankind's gametes and embryo are carried out observation in vitro and operation during on incubator temperature and the impact of gas concentration, at utmost maintain the potentiality of development of himself.
Utility model content
This utility model is intended to overcome the deficiencies in the prior art, it is provided that a kind of culture dish transferring pallet, and what this device can be convenient and safe is disposable by greater number of culture dish transhipment turnover incubator, ensure that heat and the exchange of gas in incubator simultaneously.
In order to achieve the above object, the technical scheme that this utility model provides is:
Described culture dish transferring pallet includes metal base plate that surface processes through frosted and the metal side plate that surface processes through frosted；Described metal base plate and metal side plate are provided with multiple air-vent.Metal material is fine aluminium, aluminium alloy or rustless steel, preferably fine aluminium.
The thickness of described metal base plate and metal side plate is 1.5 2mm.
Described vent diameter is 7 9mm, preferably 8mm；Distance between adjacent air-vent is 1.5 2.5mm, preferably 2mm.Air-vent is uniform intervals distribution on metal base plate, is single distribution in metal side plate.
The size of transferring pallet can be customized according to the type of used culture dish, and as a example by the 60mm culture dish (57mm × 15mm) that laboratory is conventional, a length of 320mm of described metal base plate, a width of 250mm, metal baffle height is 20mm.Each pallet can place 20 60mm culture dishs.
Below in conjunction with principle, the utility model is described in further detail:
This utility model use the light-weight metal (such as fine aluminium etc.) that heat transfer efficiency is high make, its weight is light, heat transfer efficiency is high, and it is uniformly distributed air-vent on metal base plate and baffle plate, ensure that pallet and culture dish are placed in incubator, the heat within incubator and gas will not be circulated ripe appreciable impact.Metal base plate and metal baffle use the disposable punch forming of sheet metal, and its intensity is high, and seamless, it is simple to clean and sterilizing.The all edges of this sheet metal all use polishing Passivation Treatment, prevent from hurting operator's skin.Sheet metal before stamping, carries out two-sided frosted process, slides, be also prevented from pallet and slide in incubator in being possible to prevent culture dish pallet.Described metal base plate is that region placed by culture dish, and metal side plate then can prevent culture dish landing in transport process.
Compared with prior art, the beneficial effects of the utility model are:
Culture dish transferring pallet of the present utility model can be convenient and safe disposable by greater number of culture dish transhipment turnover incubator, the light-weight metal of heat transfer efficiency height and equally distributed air-vent simultaneously, it is possible to ensure heat and the exchange of gas in incubator.Overcome the most manual transhipment culture dish inefficiency and poor stability, and the shortcoming that conventional medical rustless steel pallet affects incubator heat and other exchanges.Its simple in construction, easy to use, with low cost, it is possible to more effective improvement on incubator temperature and the impact of gas concentration, at utmost maintains the potentiality of development of himself during a large amount of mankind's gametes and embryo carry out observation in vitro and operation.
This utility model except be applicable to external fertilization laboratory for mankind's gamete and the transhipment of ware embryo culture, being also applied for other needs to carry out the laboratorys such as the cytobiology of cell, tissue, organ and microorganism culturing, fetology, zoology and microbiology.
Below in conjunction with drawings and Examples, this utility model is further elaborated.
Accompanying drawing explanation
Fig. 1 is this utility model top view；
Fig. 2 is the A-A sectional view of Fig. 1.
In figure: 1, metal base plate；2, air-vent；3, metal side plate.
Detailed description of the invention
Embodiment 1
Seeing Fig. 1 and Fig. 2, described culture dish transferring pallet includes metal base plate 1 that surface processes through frosted and the metal side plate 3 that surface processes through frosted；Described metal base plate 1 and metal side plate 3 are provided with multiple air-vent 2.The thickness of described metal base plate 1 and metal side plate 3 is 1.5 2mm.The metal material of described metal base plate 1 and metal side plate 3 is fine aluminium.The described a diameter of 8mm of air-vent 2, the distance between adjacent air-vent 2 is 2mm, and air-vent 2 is uniform intervals distribution on metal base plate, is single distribution in metal baffle.
The content that above-described embodiment illustrates should be understood to that these embodiments are only used for being illustrated more clearly that this utility model, rather than limit scope of the present utility model, after having read this utility model, those skilled in the art each fall within the application claims limited range to the amendment of the various equivalent form of values of the present utility model.
Claims (3)
1. a culture dish transferring pallet, it is characterised in that described culture dish transferring pallet includes metal base plate (1) that surface processes and the metal side plate (3) that surface processes through frosted through frosted；Described metal base plate (1) and metal side plate (3) are provided with multiple air-vent (2).
2. culture dish transferring pallet as claimed in claim 1, it is characterised in that the thickness of described metal base plate (1) and metal side plate (3) is 1.5 2mm.
3. culture dish transferring pallet as claimed in claim 1, it is characterised in that a diameter of 7 9mm of described air-vent (2), the distance between adjacent air-vent (2) is 1.5 2.5mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620020108.1U CN205603569U (en) | 2016-01-11 | 2016-01-11 | Tray is transported to culture dish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620020108.1U CN205603569U (en) | 2016-01-11 | 2016-01-11 | Tray is transported to culture dish |
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| Publication Number | Publication Date |
|---|---|
| CN205603569U true CN205603569U (en) | 2016-09-28 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620020108.1U Expired - Fee Related CN205603569U (en) | 2016-01-11 | 2016-01-11 | Tray is transported to culture dish |
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| Country | Link |
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| CN (1) | CN205603569U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107739712A (en) * | 2017-09-30 | 2018-02-27 | 重庆市妇幼保健院 | Suitable for Embryo Culture and the device of identification |
-
2016
- 2016-01-11 CN CN201620020108.1U patent/CN205603569U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107739712A (en) * | 2017-09-30 | 2018-02-27 | 重庆市妇幼保健院 | Suitable for Embryo Culture and the device of identification |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160928 Termination date: 20190111 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |