CN205603601U - Culture vessel - Google Patents
Culture vessel Download PDFInfo
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- CN205603601U CN205603601U CN201620020107.7U CN201620020107U CN205603601U CN 205603601 U CN205603601 U CN 205603601U CN 201620020107 U CN201620020107 U CN 201620020107U CN 205603601 U CN205603601 U CN 205603601U
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- embryo
- vitro
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- culture
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Abstract
The utility model discloses a culture vessel. Culture vessel includes ware lid and dish body, the inboard bottom surface of dish body is equipped with a plurality of arc sunk area. This arc sunk area can utilize the action of gravity to be in the same place be convenient for external observation and operation with oocyte with embryo's gathering. Avoid ordinary culture vessel because the bottom is flat, leaded to the difficult shortcoming of looking for of human oocyte and embryo's reciprocal dispersion. Its simple structure, convenient to use, low cost, the efficiency of can comparatively effectual improvement carrying out external observation and operation to human oocyte and embryo is showing the rate of utilization that improves culture vessel simultaneously. The utility model discloses except being applicable to in the laboratory in vitro fertilization to human oocyte and external operation of embryo and in vitro culture, also be applicable to the embryology and the experiment of zoology room of carrying out external operation and in vitro culture to the oocyte and the embryo of other species.
Description
Technical field
This utility model belongs to biotic experiment apparatus field, is specifically related to a kind of culture dish.
Background technology
Estimating according to the World Health Organization (WHO), in developed country, the couple at child-bearing age of 10-15% is perplexed by infertility.In developing country (including China), the women of child-bearing age having 1.86 hundred million to 2002 suffer from infertility.The statistical data of 2014 shows, the incidence rate of China's reproductive population infertility is 12.5%, and infertility number is more than 40,000,000.Vitro fertilization-embryo implanting (in-vitro
Fertilization and embryo transfer, IVF-ET) technology is the important means treating infertility at present.From 1978 since Britain's birth is the first " test-tube baby " in the world, current developed country has accounted for the 1-3% of natus sum by the baby that ART gravidity assisting is born, and estimates that the births of whole world test-tube baby are more than 5,000,000 people.
In vitro during fertilization-embryo transfer technology implementation, need in vitro human oocytes and embryo to be carried out In vitro culture.In vitro culture is carried out in culture dish, it is to use common Tissue Culture Dish (such as 35mm that current most of external fertilization laboratory carries out oocyte with the In vitro culture of embryo, 60mm Petri dish, 4 orifice plates etc.), it is pure flat at the bottom of the ware of these culture dishs.And human oocytes and embryo be subglobular and volume the most small, wherein human oocytes, germ cell and cleavage stage embryo's diameter about 120-150 micron, blastaea about 200-300 micron.Owing to having the protection of zona pellucida, human oocytes and embryo that the adherent growth that also ordinary cells is the same also will not occur, therefore it is very easy to roll.The patient carrying out outcome of in vitro fertilization-embryo transfer in treatment often has multiple oocyte and embryo (10-20 the most) to need to carry out In vitro culture and manipulation in vitro, in the culture dish that bottom is pure flat, these oocytes and embryo are very easy to occur in the medium to roll and be scattered in each corner of culture dish.Such as, when embryo transfer, need to be placed on 2-3 piece of embryo in single hole organ culture's ware that internal diameter about 15mm contains 1ml culture medium, slightly rock embryo and will be rolled to the edge of endoporus, so these embryos are sucked transfer catheter when, the difficulty of operation will be increased, be very easy to suck too much culture medium.Additionally; embryo cryopreservation thaws when; even need to be transferred in the hole of 4 orifice plates the embryo of more than 10 pieces successively; each hole internal diameter about 10mm contains the different types of cryoprotective agent of 0.5ml; the most slightly rock embryo and will be rolled to the edge in hole; so these embryos are transferred to another hole from a hole when, it will increase the difficulty of bigger operation, it is difficult to ensure that the same stipulated time is by complete for all embryos transfer.
It is the most frequently used method embryo culture that microdroplet is cultivated, it is simply that drop in bottom culture dish by the culture medium of 10-50 microlitre, then covers paraffin oil above, oocyte and embryo are placed in culture medium microdroplet cultivation.Microdroplet is cultivated a certain degree of can improve the problems referred to above.But the volume that microdroplet cultivation also has certain limitation, first microdroplet can not too greatly (not can exceed that 100 microlitres), and otherwise the surface tension of culture medium can not maintain the shape of drop, slightly rocks microdroplet and will disperse.Therefore, some need to cultivate the more operation of base unit weight and are not suitable for using microdroplet to cultivate, the collection of such as oocyte, embryo transfer and oocyte and embryo cryopreservation defrosting etc..Secondly, in flat culture dish, carry out microdroplet cultivate the rolling that also cannot avoid oocyte and embryo completely, and mobile microtitre plate to need nonetheless remain for action soft, slightly larger being likely to of strength causes microdroplet dispersion, oocyte and embryo may be floated and be scattered in other microdroplets by scattered culture medium microdroplet, cause sample mix (embryo of such as normal fertilization and abnormal fertilization obscures), or floating to paraffin oil surface causes sample to be lost.
Therefore, how can overcome in flat culture dish, multiple oocytes and embryo roll and mutually disperse, and cause manipulation in vitro and observation in vitro difficulty to increase, and are the difficult problems that solve the most very well of most external fertilization laboratory.Solve this difficult problem, can will more be effectively improved operator's speed of operation when multiple human oocytes and embryo being carried out manipulation in vitro and In vitro culture, and significantly reduce operation easier.
Utility model content
This utility model is intended to overcome the deficiencies in the prior art, it is provided that a kind of culture dish, this device can utilize action of gravity by oocyte together with embryo aggregation, it is simple to observation in vitro and operation.
In order to achieve the above object, the technical scheme that this utility model provides is:
Described culture dish includes ware lid and ware body；Described ware body inside bottom surface is provided with several arc-shaped recess regions.
The culture dish of described bottom arc and conventional Petri dish profile substantially close to, simply ware body inside bottom surface is provided with the arc-shaped recess region that quantity does not waits.
Wherein, described ware base thickness degree is 4mm, and the quantity in arc-shaped recess region is respectively 1 (single hole arc ware), 4 (4 radial shape wares) and 16 (16 radial shape ware), replaces traditional single hole organ culture's ware, four orifice plates and the culture dish of making microdroplet respectively.
Wherein, the culture dish of described bottom arc is identical with conventional Petri dish material, for various types of plastics, such as polypropylene, polystyrene or Merlon, optimization polypropylene.
The size of culture dish, and the size in arc-shaped recess district can be customized as required with quantity, as a example by the 60mm culture dish (57mm × 15mm) that laboratory is conventional: a height of 10mm of ware lid, external diameter is 57mm, and thickness is 1mm, and internal diameter is 55mm；Ware height is 14mm, and external diameter is 53mm, and ware body edge enclosing thickness internal diameter is 1mm, and ware base thickness degree is 4mm；Wherein single hole arc ware, arc-shaped recess regional diameter is 40 ± 2mm, the central authorities a width of 3 ± 0.5mm of depth capacity；4 radial shape wares, arc-shaped recess regional diameter is 20 ± 2mm, the central authorities a width of 2 ± 0.5mm of depth capacity；16 radial shape wares, arc-shaped recess regional diameter is 10 ± 2mm, the central authorities a width of 1 ± 0.5mm of depth capacity.
Below in conjunction with principle, the utility model is described in further detail:
This utility model is identical with conventional Petri dish material, for various types of plastics (such as polypropylene, polystyrene or Merlon, optimization polypropylene), profile also with conventional Petri dish substantially close to.Therefore, compared with cellar culture ware, this utility model operate in vitro with In vitro culture during, extra harmful effect will not be brought.This utility model ware base thickness degree is about 4mm, has the arc-shaped recess region that quantity does not waits, respectively 1 (single hole arc ware), 4 (4 radial shape wares) and 16 (16 radial shape ware) at the bottom of ware respectively.Single hole arc ware, 4 radial shape wares and 16 radial shape wares replace traditional single hole organ culture's ware, four orifice plates and the culture dish of making microdroplet respectively.During multiple human oocytes and embryo are carried out manipulation in vitro and In vitro culture, these oocytes and embryo due to action of gravity, can be gathered in the central authorities in arc-shaped recess region, are therefore very beneficial for carrying out observation in vitro and operation.
Compared with prior art, the beneficial effects of the utility model are:
This utility model utilizes action of gravity, and when carrying out manipulation in vitro and In vitro culture, oocyte and embryo can be gathered in the arc-shaped recess region central authorities bottom culture dish, are therefore very beneficial for carrying out observation in vitro and operation.Overcome conventional culture dish due to pure flat at the bottom of ware, and cause oocyte and embryo mutually to disperse due to rolling, cause the shortcoming carrying out manipulation in vitro and observing difficulty.Its simple in construction, easy to use, and without any harmful effect, it is possible to more it is effectively improved operator's speed of operation when multiple human oocytes and embryo being carried out manipulation in vitro and In vitro culture, and significantly reduces operation easier.
In a word, the arc-shaped recess region bottom this culture dish, it is possible to utilize action of gravity by oocyte together with embryo aggregation, it is simple to observation in vitro and operation.Simultaneously by dividing multiple arc-shaped recess districts at the bottom of ware, it is possible to set up different operations in same culture dish and cultivate region.Avoid Nostoc commune Vanch ware due to bottom pure flat, cause human oocytes and embryo mutually to disperse to be difficult to situation about finding.Its simple in construction, easy to use, with low cost, it is possible to more effectively to improve the efficiency that human oocytes and embryo are carried out observation in vitro and operation, significantly improve the utilization rate of culture dish simultaneously.
This utility model except be applicable to external fertilization laboratory for human oocytes and embryo's manipulation in vitro and In vitro culture, be also applied for the oocyte for other species and embryo carry out manipulation in vitro and the fetology of In vitro culture and Experiment of Zoology room.
Accompanying drawing explanation
Fig. 1 is the culture dish top view with 1 arc-shaped recess region；
Fig. 2 is the A-A sectional view of Fig. 1；
Fig. 3 is the culture dish top view with 4 arc-shaped recess regions；
Fig. 4 is the A-A sectional view of Fig. 3；
Fig. 5 is the culture dish top view with 16 arc-shaped recess regions；
Fig. 6 is the A-A sectional view of Fig. 5.
In figure: 1, ware lid；2, ware body；3, arc-shaped recess region.
Detailed description of the invention
Embodiment 1
Seeing Fig. 1 and 2, described culture dish includes ware lid 1 and ware body 2；The a height of 10mm of ware lid 1, external diameter is 57mm, and thickness is 1mm, and internal diameter is 55mm；The a height of 14mm of ware body 2, external diameter is 53mm, and ware body 2 edge enclosing thickness internal diameter is 1mm, and the ware base thickness degree of ware body 2 is 4mm；Described ware body 2 inside bottom surface is provided with 1 arc-shaped recess region 3；A diameter of 40mm in described arc-shaped recess region 3, center depth capacity is 3mm.
Embodiment 2
Seeing Fig. 3 and 4, described culture dish includes ware lid 1 and ware body 2；The a height of 10mm of ware lid 1, external diameter is 57mm, and thickness is 1mm, and internal diameter is 55mm；The a height of 14mm of ware body 2, external diameter is 53mm, and ware body 2 edge enclosing thickness internal diameter is 1mm, and the ware base thickness degree of ware body 2 is 4mm；Described ware body 2 inside bottom surface is provided with 4 arc-shaped recess regions 3；A diameter of 20mm in described arc-shaped recess region 3, center depth capacity is 2mm.
Embodiment 3
Seeing Fig. 5 and 6, described culture dish includes ware lid 1 and ware body 2；The a height of 10mm of ware lid 1, external diameter is 57mm, and thickness is 1mm, and internal diameter is 55mm；The a height of 14mm of ware body 2, external diameter is 53mm, and ware body 2 edge enclosing thickness internal diameter is 1mm, and the ware base thickness degree of ware body 2 is 4mm；Described ware body 2 inside bottom surface is provided with 1 arc-shaped recess region 3；A diameter of 10mm in described arc-shaped recess region 3, center depth capacity is 1mm.
The content that above-described embodiment illustrates should be understood to that these embodiments are only used for being illustrated more clearly that this utility model, rather than limit scope of the present utility model, after having read this utility model, those skilled in the art each fall within the application claims limited range to the amendment of the various equivalent form of values of the present utility model.
Claims (1)
1. a culture dish, including ware lid (1) and ware body (2)；It is characterized in that, described ware body (2) inside bottom surface is provided with 1 arc-shaped recess region (3), a diameter of 40 ± 2mm of described arc-shaped recess region (3), and center depth capacity is 3 ± 0.5mm；Or, described ware body (2) inside bottom surface is provided with 4 equally distributed arc-shaped recess regions (3), a diameter of 20 ± 2mm of described arc-shaped recess region (3), and center depth capacity is 2 ± 0.5mm；Or, described ware body (2) inside bottom surface is provided with 16 equally distributed arc-shaped recess regions (3), a diameter of 10 ± 2mm of described arc-shaped recess region (3), and center depth capacity is 1 ± 0.5mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620020107.7U CN205603601U (en) | 2016-01-11 | 2016-01-11 | Culture vessel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620020107.7U CN205603601U (en) | 2016-01-11 | 2016-01-11 | Culture vessel |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205603601U true CN205603601U (en) | 2016-09-28 |
Family
ID=56961077
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620020107.7U Expired - Fee Related CN205603601U (en) | 2016-01-11 | 2016-01-11 | Culture vessel |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205603601U (en) |
-
2016
- 2016-01-11 CN CN201620020107.7U patent/CN205603601U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160928 Termination date: 20190111 |