CN104560694A - Microbiological culture medium device and operating method thereof - Google Patents
Microbiological culture medium device and operating method thereof Download PDFInfo
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- CN104560694A CN104560694A CN201510048589.7A CN201510048589A CN104560694A CN 104560694 A CN104560694 A CN 104560694A CN 201510048589 A CN201510048589 A CN 201510048589A CN 104560694 A CN104560694 A CN 104560694A
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- scriber
- based devices
- lid
- substratum
- microorganism culturing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
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- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
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- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
For solving the problems of low biological safety, non-standardized operation, large manual operation randomness, high human cost, low efficiency, low result recognition rate and the like existing in microbiological culture medium devices and operating methods thereof in the prior art, the invention discloses a microbiological culture medium device and an operating method thereof. The microbiological culture medium device disclosed by the invention comprises a culture medium and a scriber. The special operation of the microbiological culture medium device disclosed by the invention is implemented according to certain steps. According to the microbiological culture medium device and the operating method thereof disclosed by the invention, the problem of biological safety is solved, the experimental environment and the operating personnel are protected, the operation is standardized, the human cost is reduced, the work efficiency is increased, the operation procedure is standardized and mechanized, and the experimental result recognition rate is increased.
Description
Technical field
The present invention relates to a kind of microorganism culturing based devices and working method thereof.
Background technology
Microorganism culturing based devices is widely used in colony inoculation, cultivation, separation, detection, and it is the important work of of the aspects such as health care, inspection and quarantine, Disease epizootic, food safety that micro-biological samples is cultivated.Current microbial inoculant, cultivation, separation, detection are cultivated microorganism culturing based devices used and are comprised: a sampling ring and a glass culture dish.The working method of described microorganism culturing based devices is:
(1) in glass culture dish, operator's hand-held sampling ring carries out artificial sterilizing;
(2) a small amount of micro-biological samples is annotated the surface of the nutrient solution in glass culture dish by sampling ring gently that be moistened with microorganism;
(3) diluted by sectional streak in media surface by the different cells in microorganism mixed in together or same microbial population by the method for line and obtain the individual cells of more independent distribution, after cultivating, growth and breeding becomes single bacterium colony.
Obviously, existing microorganism culturing based devices and working method thereof have following shortcoming:
(1) when operating, testing staff is according to the sensation of hand, the end of transfering loop is made just to touch the surface of nutrient solution, then cultivate at a certain temperature, after bacterial growth is large, then observe with proofing unit, detect colony number wherein, do not have stability, the arbitrariness of line is higher;
(2) due to adopt manual, make transfering loop just touch the surface of nutrient solution with feeling, time and contact the surface of nutrient solution, time and the phenomenon left is difficult to avoid, easy cross infection or bacteria distribution poor effect;
(3) owing to adopting artificial hatched manner, cause result can not stdn, experimental result recognizes difficulty again, judges inaccurate;
(4) manual operation is wasted time and energy, inefficiency;
(5) have potential biological pollution and hazardness, operator easily come to harm in open experimental situation.
Generally speaking, the microorganism culturing based devices of prior art and working method thereof also exist that biological safety is low, operation can not stdn, manual operation random large, human cost is high and inefficiency, experimental result recognize the low problem of rate again.
Summary of the invention
In order to solve, the biological safety that prior art microorganism culturing based devices and working method thereof exist is low, operation can not stdn, manual operation random large, human cost high-level efficiency is low, result recognizes the problems such as rate is low again, the present invention proposes a kind of microorganism culturing based devices and working method thereof.
Microorganism culturing based devices of the present invention, comprise substratum and scriber, described substratum comprises box body, lid, spring, buckle and deck, described box body and lid with the use of and chain connection, namely in hinged hinged place mounting spring, when described spring makes lid open, state is held open; Described box body is also provided with deck, described lid is also provided with buckle, described buckle has latch, described buckle is fastened by latch and deck, is kept closed when lid is closed; Described spring is torsion spring or top spring; Described scriber comprises handle, crossbeam and transfering loop, the top of described handle is inboardend, and be detachably connected with the mechanical mobile device of exterior mechanical auto-plant, the other end of described handle is fixedly connected with described crossbeam, described crossbeam is fixedly installed at least one transfering loop.
Further, described spring is torsion spring or top spring;
Further, the position of described buckle and deck can exchange;
Further, described box body and with it with the use of the profile of lid can be: circular, square, prismatic or ellipse;
Further, described transfering loop is 1 to 8;
Further, described transfering loop is ring-type or spherical.
When concrete operations microorganism culturing based devices of the present invention, its step is as follows:
(1) on the box body of exterior mechanical bar touching substratum, the deck that arranges makes buckle unclamp, lid by spring with box body in opening afterwards and keeping this opened condition;
(2) open exterior mechanical auto-plant, its mechanical mobile device drives the handle of scriber of the present invention to move according to the program arranged, and meanwhile, substratum is positioned at the below of scriber by transport unit;
(3) scriber sampling, namely mechanical mobile device band fixed handle dips micro-biological samples by described transfering loop, and rules on microorganism culturing based devices;
(4), after line, scriber leaves;
(5) close substratum, the exterior mechanical bar being namely positioned at top touches lid, and the deck that the buckle of lid and box body are arranged fastens, and is kept closed when substratum is closed;
(6) scriber that used of mechanical mobile device automatic dismantling, and obtain another new scriber;
(7) substratum having completed line is left by transport unit, and next new substratum 1 is positioned at the below of scriber by transport unit;
(8) above-mentioned steps () is repeated to (seven).
Further, the working method of microorganism culturing based devices of the present invention, described step () and step (two) can be exchanged.
The Advantageous Effects of microorganism culturing based devices of the present invention and working method thereof is: biosafety issues is solved; Protection environment and operator, operational standardization, human cost reduces; working efficiency increases, operating process standardized mechanical, and experimental result recognizes rate again to be increased.
Accompanying drawing explanation
Accompanying drawing 1 is the structural representation of substratum of the present invention;
Accompanying drawing 2 is the closedown shape body schematic diagram of substratum of the present invention;
Accompanying drawing 3 is the unlatching shape body schematic diagram of substratum of the present invention;
Accompanying drawing 4 is a kind of embodiment schematic diagram of substratum of the present invention;
Accompanying drawing 5 is the structural representation of scriber of the present invention;
Accompanying drawing 6 is the structural representation of microorganism culturing based devices of the present invention;
Below in conjunction with the drawings and specific embodiments, microorganism culturing based devices of the present invention is further described.
Embodiment
Accompanying drawing 1 is the structural representation of microorganism culturing based devices of the present invention, accompanying drawing 2 is the closedown shape body schematic diagram of microorganism culturing based devices of the present invention, accompanying drawing 3 is the unlatching shape body schematic diagram of microorganism culturing based devices of the present invention, accompanying drawing 4 is a kind of embodiment schematic diagram of microorganism culturing based devices of the present invention, accompanying drawing 5 is the structural representation of scriber of the present invention, accompanying drawing 6 is the structural representation of microorganism culturing based devices of the present invention, in figure, 1 is substratum, 2 is scriber, 1.1 is box body, 1.2 is lid, 1.3 is spring, 1.4 is buckle, 1.5 is deck, 2.1 is handle, 2.2 is crossbeam, 2.3 is transfering loop.As shown in the figure, microorganism culturing based devices of the present invention, comprise substratum 1 and scriber 2, described substratum 1 comprises box body 1.1, lid 1.2, spring 1.3, buckle 1.4 and deck 1.5, described box body 1.1 and lid 1.2 with the use of and chain connection, namely in hinged hinged place mounting spring 1.3, when described spring 1.3 makes lid 1.2 open, state is held open; Described box body 1.1 is also provided with deck 1.5, described lid 1.2 is also provided with buckle 1.4, described buckle 1.4 has latch, described buckle 1.4 is fastened by latch and deck 1.5, is kept closed when lid 1.2 is closed; Described spring 1.3 is torsion spring or top spring; Described scriber 2 comprises handle 2.1, crossbeam 2.2 and transfering loop 2.3, the top of described handle 2.1 is inboardend, and be detachably connected with the mechanical mobile device of exterior mechanical auto-plant, the other end of described handle 2.2 is fixedly connected with described crossbeam 2.2, described crossbeam 2.2 is fixedly installed at least one transfering loop 2.3.
Play opening/closing lid 1.2 and the effect holding on/off shape body to better reach, spring 1.3 of the present invention is torsion spring or top spring; In the specific implementation, in order to the requirement of the existing exterior mechanical auto-plant of more convenient cooperation, the position of described buckle 1.4 and deck 1.5 can exchange; Same, it will be appreciated by persons skilled in the art that the outer shape of substratum 1 does not affect implementation result of the present invention, therefore, described box body 1.1 and with it with the use of the profile of lid 1.2 can be: circular, square, prismatic or ellipse; In order to better increase work efficiency, described transfering loop 2.3 is 1 to 8; In order to play effect of better ruling, described transfering loop 2.3 is ring-type or spherical.In the specific implementation, microorganism culturing based devices of the present invention can be that one to four cover operates simultaneously, for same microbiological specimens, can carry out many groups at one time and detects and judge, increase working effect.
When concrete operations microorganism culturing based devices of the present invention, its step is as follows:
(1) deck 1.5 that the box body 1.1 of exterior mechanical bar touching substratum 1 is arranged makes buckle 1.4 unclamp, lid 1.2 by spring 1.3 and box body 1.1 in keeping this opened condition after opening;
(2) open exterior mechanical auto-plant, its mechanical mobile device drives the handle 2.1 of scriber 2 of the present invention to move according to the program arranged, and meanwhile, substratum 1 is positioned at the below of scriber 2 by transport unit;
(3) scriber 2 samples, and namely mechanical mobile device band fixed handle 2.1 dips micro-biological samples by described transfering loop 2.3, and rules on microorganism culturing based devices;
(4), after line, scriber 2 leaves;
(5) close substratum 1, the exterior mechanical bar being namely positioned at top touches lid 1.2, and the deck 1.5 that the buckle of lid 1.2 1.4 and box body 1.1 are arranged fastens, and is kept closed when substratum 1 is closed;
(6) scriber 2 that used of mechanical mobile device automatic dismantling, and obtain another new scriber 2;
(7) substratum 1 having completed line is left by transport unit, and next new substratum 1 is positioned at the below of scriber 2 by transport unit;
(8) above-mentioned steps () is repeated to (seven).
Further, the working method of microorganism culturing based devices of the present invention, described step () and step (two) can be exchanged.In the specific implementation, the travel direction of scriber, according to shown in accompanying drawing 6, moves to B by A, then moves to C, then move to D, thus complete above-mentioned scratching process.
Obviously; the advantage that microorganism culturing based devices of the present invention has low price, cooperation thought by easy to operate, not fragile, easy and multiple unlatching exterior mechanical auto-plant; simultaneously; the working method of microorganism culturing based devices of the present invention makes biosafety issues be solved; and Protection environment and operator, operational standardization, human cost reduces; working efficiency increases, operating process standardized mechanical, and experimental result recognizes rate again to be increased.
Claims (8)
1. a microorganism culturing based devices, it is characterized in that, comprise substratum (1) and scriber (2), described substratum (1) comprises box body (1.1), lid (1.2), spring (1.3), buckle (1.4) and deck (1.5), described box body (1.1) and lid (1.2) with the use of and chain connection, namely in hinged hinged place mounting spring (1.3), when described spring (1.3) makes lid (1.2) open, state is held open; Described box body (1.1) is also provided with deck (1.5), described lid (1.2) is also provided with buckle (1.4), (1.4) have latch to described buckle, and described buckle (1.4) is fastened by latch and deck (1.5), is kept closed when lid (1.2) is closed; Described spring (1.3) is torsion spring or top spring; Described scriber (2) comprises handle (2.1), crossbeam (2.2) and transfering loop (2.3), the top of described handle (2.1) is inboardend, and be detachably connected with the mechanical mobile device of exterior mechanical auto-plant, the other end of described handle (2.2) is fixedly connected with described crossbeam (2.2), described crossbeam (2.2) is fixedly installed at least one transfering loop (2.3).
2. microorganism culturing based devices according to claim 1, is characterized in that, described spring (1.3) is torsion spring or top spring.
3. microorganism culturing based devices according to claim 1, is characterized in that, the position of described buckle (1.4) and deck (1.5) can exchange.
4. microorganism culturing based devices according to claim 1, is characterized in that, described box body (1.1) and with it with the use of the profile of lid (1.2) can be: circular, square, prismatic or ellipse.
5. microorganism culturing based devices according to claim 1, is characterized in that, described transfering loop (2.3) is 1 to 8.
6. microorganism culturing based devices according to claim 1, is characterized in that, described transfering loop (2.3) is ring-type or spherical.
7., when concrete operations microorganism culturing based devices of the present invention, the working method of microorganism culturing based devices, is characterized in that, carries out successively as follows:
(1) the upper deck (1.5) arranged of the box body (1.1) of exterior mechanical bar touching substratum 1 makes buckle (1.4) unclamp, lid (1.2) by spring (1.3) and box body (1.1) in keeping this opened condition after opening;
(2) open exterior mechanical auto-plant, its mechanical mobile device drives the handle (2.1) of scriber 2 of the present invention to move according to the program arranged, and meanwhile, substratum 1 is positioned at the below of scriber 2 by transport unit;
(3) scriber 2 samples, and namely mechanical mobile device band fixed handle (2.1) dips micro-biological samples by described transfering loop (2.3), and rules on microorganism culturing based devices;
(4), after line, scriber 2 leaves;
(5) close substratum 1, the exterior mechanical bar being namely positioned at top touches lid (1.2), and the buckle (1.4) of lid (1.2) fastens with the upper deck (1.5) arranged of box body (1.1), is kept closed when substratum 1 is closed;
(6) scriber 2 that used of mechanical mobile device automatic dismantling, and obtain another new scriber 2;
(7) substratum 1 having completed line is left by transport unit, and next new substratum 1 is positioned at the below of scriber 2 by transport unit;
(8) above-mentioned steps () is repeated to (seven).
8. the working method of microorganism culturing based devices according to claim 7, described step () and step (two) can be exchanged.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510048589.7A CN104560694A (en) | 2015-01-30 | 2015-01-30 | Microbiological culture medium device and operating method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510048589.7A CN104560694A (en) | 2015-01-30 | 2015-01-30 | Microbiological culture medium device and operating method thereof |
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| Publication Number | Publication Date |
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| CN104560694A true CN104560694A (en) | 2015-04-29 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510048589.7A Pending CN104560694A (en) | 2015-01-30 | 2015-01-30 | Microbiological culture medium device and operating method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111269836A (en) * | 2020-02-28 | 2020-06-12 | 山东商业职业技术学院 | Intelligent microorganism streak skill culture method |
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| CN102757891A (en) * | 2011-04-28 | 2012-10-31 | 魏志勇 | Automatic microbial inoculation instrument |
| CN202786239U (en) * | 2012-08-16 | 2013-03-13 | 王晨 | Culture dish |
| CN202989137U (en) * | 2012-11-30 | 2013-06-12 | 淄博市张店区人民医院 | Inoculating loop |
| CN203096078U (en) * | 2013-03-14 | 2013-07-31 | 华珊微生物科技有限公司 | Microbial sample processing system |
| CN103508088A (en) * | 2013-10-11 | 2014-01-15 | 重庆道鑫生物科技有限公司 | Device for microorganism sample collection |
| CN203807471U (en) * | 2014-04-24 | 2014-09-03 | 山东省农业科学院玉米研究所 | Automatic batch inoculation device |
| CN204474670U (en) * | 2015-01-30 | 2015-07-15 | 重庆道鑫生物科技有限公司 | A kind of microorganism culturing based devices |
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2015
- 2015-01-30 CN CN201510048589.7A patent/CN104560694A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102757891A (en) * | 2011-04-28 | 2012-10-31 | 魏志勇 | Automatic microbial inoculation instrument |
| CN202201902U (en) * | 2011-09-22 | 2012-04-25 | 苏州先阔生物科技有限公司 | Inoculating loop |
| CN202786239U (en) * | 2012-08-16 | 2013-03-13 | 王晨 | Culture dish |
| CN202989137U (en) * | 2012-11-30 | 2013-06-12 | 淄博市张店区人民医院 | Inoculating loop |
| CN203096078U (en) * | 2013-03-14 | 2013-07-31 | 华珊微生物科技有限公司 | Microbial sample processing system |
| CN103508088A (en) * | 2013-10-11 | 2014-01-15 | 重庆道鑫生物科技有限公司 | Device for microorganism sample collection |
| CN203807471U (en) * | 2014-04-24 | 2014-09-03 | 山东省农业科学院玉米研究所 | Automatic batch inoculation device |
| CN204474670U (en) * | 2015-01-30 | 2015-07-15 | 重庆道鑫生物科技有限公司 | A kind of microorganism culturing based devices |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111269836A (en) * | 2020-02-28 | 2020-06-12 | 山东商业职业技术学院 | Intelligent microorganism streak skill culture method |
| CN111269836B (en) * | 2020-02-28 | 2022-03-15 | 山东商业职业技术学院 | Intelligent microorganism streak skill culture method |
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