CN203807471U - Automatic batch inoculation device - Google Patents

Automatic batch inoculation device Download PDF

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Publication number
CN203807471U
CN203807471U CN201420202630.2U CN201420202630U CN203807471U CN 203807471 U CN203807471 U CN 203807471U CN 201420202630 U CN201420202630 U CN 201420202630U CN 203807471 U CN203807471 U CN 203807471U
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CN
China
Prior art keywords
test tube
culture dish
worktable
conveying belt
inoculated
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn - After Issue
Application number
CN201420202630.2U
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Chinese (zh)
Inventor
贾曦
赵海军
张成华
杨菲
王鹏
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Maize Research Institute of Shandong Academy of Agricultural Sciences
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Maize Research Institute of Shandong Academy of Agricultural Sciences
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Priority to CN201420202630.2U priority Critical patent/CN203807471U/en
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Abstract

The utility model discloses an automatic batch inoculation device comprising a workbench, wherein a culture dish fixing rack and a to-be-inoculated test tube mounting rack are fixed on the workbench, test tube holders are unfolded at the turning position of a conveying belt and folded at the non-turning position, a driving motor drives driving rollers to rotate, the conveying belt passes through a space below a culture dish, and a sensor is arranged right below the culture dish, a collecting mechanism for collecting a test tube is arranged on the workbench at the front end of the conveying belt, and an annular sterile zone formed by a plurality of air outlets and taking an orifice of a to-be-inoculated test tube as the center is formed on the workbench; when the test tube is mounted, a seed taking side of the culture dish and the orifice of the to-be-inoculated test tube are positioned in the sterile zone formed above the air outlets. A hot air bin, a heating bin and an air flue are arranged on the lower side of the workbench. The experimental device, namely the automatic batch inoculation device with the structure can be used for reducing the pollution rate in the sterile operation process, improving the safety, avoiding personal injury and protecting the safety of an experimental material.

Description

A kind of batch classification inoculation apparatus of automatization
Technical field
This scheme relates to a kind of use for laboratory equipment technical field, the batch classification inoculation apparatus of specifically a kind of automatization.
Background technology
There is no in the market in aseptic technique and be inoculated in batches the testing apparatus in test tube from culture dish, all to adopt manual operations to carry out at present, utilize Bechtop and spirit lamp to assist to create gnotobasis, operating process is: clamp culture dish with hand, with large finger and middle finger unlatching ware lid, picking mycelia or bacterium piece, then cover ware lid, culture dish is inverted on table top, then is picked up wait inoculating test tube and pull out after tampon is put into bacterial classification and cover again tampon.Inoculation is exactly to repeat above process in batches.Culture dish picks and places repeatedly on table top, and open and close operates the aseptic area that the unskilled test tube mouth that makes bacterium piece or the mycelia of picking and pull out tampon is built away from spirit lamp, and causing polluting probability increases.Repeatedly picking and placeing culture dish and test tube also makes efficiency lower.
In aseptic technique, the most important thing is contaminated solution problem, inoculation also will solve efficiency in batches.
(1) in operating process contaminated solution most critical be to prevent that the bacterium piece of picking or mycelia are exposed to airborne overlong time, another is to reduce as far as possible in vitro substratum with the duration of contact of air.Object is all to avoid airborne miscellaneous bacteria to take advantage of a weak point to cause polluting.The time that the mycelia of existing manual operation picking or bacterium piece expose in air is longer, and the absolutesterility region that spirit lamp is built is less, is easy to just free this region, and polluting probability increases.
(2) spirit lamp is easily overturned alcohol in use procedure and is leaked and cause fire and personal injury, also can damage experiment material.
(3) in seeded process, will repeatedly pick and place culture dish and test tube to be inoculated in batches, efficiency is lower.
Utility model content
In order to solve the deficiencies in the prior art, the utility model provides a kind of batch classification inoculation apparatus of automatization, is a kind of laboratory inoculation utility appliance of semi-automation.
The utility model solves the technical scheme that its technical problem adopts: a kind of batch classification inoculation apparatus of automatization, comprise worktable, and it is characterized in that:
On described worktable, be fixed with culture dish anchor and test tube erecting frame to be inoculated, described culture dish anchor comprises base, support cylinder, platform and culture dish cover opening mechanism, described base through screws is fixed on left-handed operation one side of worktable, described support cylinder upwards extends and forms a space of dodging below test tube to be inoculated, be fixed on the upper end of support cylinder for the described platform of culture dish holding, a hinged lever on described support cylinder, one end of described lever is provided with a sucker that carries out adhesive with culture dish lid; On described platform, install can free adjustment angle metallic bellows, and clip that can holding test tubes tampon in the free end setting of metallic bellows,
Fix a horizontality conveyer belt mechanism along worktable fore-and-aft direction, described conveyer belt mechanism comprises conveying belt, live roll and drive-motor, the working face of described conveying belt moves to far-end from operator's near-end, and on described conveying belt, be provided with for the fixing test-tube bolder of test tube to be inoculated, described test-tube bolder opens at conveying belt turning point, close up at non-turning point, described drive-motor drives described live roll to rotate, and described conveying belt is through the below of culture dish, and under culture dish, be provided with the sensor whether putting in place for detection of test tube to be inoculated, be arranged on the left-hand side of worktable for controlling the button of drive-motor work, on the worktable of described conveying belt front end, establish a collecting mechanism of collecting test tube,
On described worktable, be provided with a ring-type aseptic area centered by the test tube mouth of pipe to be inoculated being made up of some air outlets, on air outlet, by the upwardly extending silica gel sheath of buckle clamping one, the outlet of described silica gel sheath is positioned at the downside of the test tube mouth of pipe to be inoculated,
When test tube is installed, described culture dish is got kind of a side, the test tube mouth of pipe to be inoculated is positioned at the aseptic area that air outlet top forms, and guarantees that uncovered position is all in absolutesterility region,
Be provided with hot blast storehouse at described worktable downside, heating cabinet and air channel, described hot blast position in storehouse is in the downside of air outlet, and between air channel and hot blast storehouse, heating cabinet is set, described air channel is communicated with heating cabinet and air suction inlet, and in air channel, be provided with to the frequency conversion fan of heating cabinet direction air blast, in described heating cabinet, be provided with the electro-heat equipment that can produce high temperature, and on worktable corresponding to described electro-heat equipment, be provided with the pin hole of a up/down perforation, the needle body of one inoculating needle is positioned at the heating core area of heating unit just through pin hole, described pin hole is in the right-hand operated side of worktable.
Further, described test-tube bolder comprises flexure strip, arc-like sheet and trip bolt, described flexure strip is have unidirectional distortion function certainly middle to the thickness of two sides spring steel plate of thickening gradually, described spring steel plate is fixed on conveying belt, described arc-like sheet can form the both sides that are fixed on flexure strip of a circular space, and has rubber pad in the inner side of arc-like sheet.
Further, described pin hole is this structure: in electro-heat equipment heating core area, right workplatform is provided with a boring, above boring correspondence, fixes a copper sheathing, has one and the coaxial tapered hole arranging of boring in described copper sheathing.
Further, described collecting mechanism comprises inclined-plane and catch tray, described catch tray is fixed on worktable, and the two ends on inclined-plane are connected on the front end and catch tray of conveying belt, inclined-plane has the top layer of damping effect, when test tube is after conveying belt front end turning point comes off, can roll down to the catch tray in front along inclined-plane smoothly, test tube catch tray adopts stainless steel to make.
Further, described electro-heat equipment is discoid or spiral helicine resistance wire.
Further, on described worktable, be provided with one and face the air outlet of drying straight up in the test tube mouth of pipe to be inoculated place.
The beneficial effects of the utility model are:
1, this device has designed the device of alternative spirit lamp, safe and reliable.And the design of aseptic blowing district design consideration flow feature, the flames of anger, can guarantee test and personnel safety.
2, in batch seeded process, test tube to be inoculated is removable, and culture dish can be fixed, and has reduced and has picked and placeed number of times, has improved efficiency.And this device is pressed close to culture dish and test tube mouth to be inoculated, bottom is with aseptic blowing device, guarantees that culture dish and test tube mouth all in aseptic area, have reduced pollution rate dramatically.
3, the test-tube bolder on test-tube stand is made by spring piece, can automated exchanged cutter and release, and go for the test tube of plurality of specifications.
4, the opening support of culture dish ware lid utilizes lever and sucker principle, and left-hand finger is stirred and can be operated, need not artificial hand-held clamping, make easy and safe to operate.
5, tampon folder that can arbitrarily angled adjusting, the defect in the absolutesterility region of having avoided dropping, dissociate in artificial gripping process.
6, this device can be fixed on test tube on test-tube stand automatically continuously, and by automatic push button vaccinization, efficiency improves greatly.
In sum, the experimental installation of this structure, can reduce the pollution rate in aseptic technique process, has improved security, avoids personal injury, Protection material safety.Meanwhile, improved the working efficiency of inoculation in batches.
Brief description of the drawings
Fig. 1 is one of stereographic map of the present utility model.
Fig. 2 is two of stereographic map of the present utility model.
Fig. 3 is side-view of the present utility model.
Fig. 4 is upward view of the present utility model.
Fig. 5 is A--A sectional view in Fig. 2.
Fig. 6 is the scheme of installation of test-tube bolder on conveying belt.
Fig. 7 is the side direction view of Fig. 6.
Fig. 8 is the turning point distortion schematic diagram of Fig. 7.
In figure: 1 worktable, 21 bases, 22 support cylinders, 23 platforms, 24 levers, 25 suckers, 31 conveying belt, 32 live rolls, 33 drive-motor, 34 bearing bases, 35 belt transmission, 36 test-tube bolders, 361 flexure strips, 362 arc-like sheets, 363 trip bolts, 364 rubber pads, 41 inclined-planes, 42 catch traies, 5 silica gel sheaths, 51 hot blast storehouses, 52 heating cabinets, 53 air channels, 54 frequency conversion fans, 55 pin holes, 6 metallic bellowss, 61 clips, 7 buttons, 71 inoculating needles, 8 test tubes to be inoculated, 81 tampons, 9 culture dish, 91 culture dish lids.
Embodiment
As shown in Figures 1 to 8,
A batch classification inoculation apparatus for automatization, comprises worktable 1, and worktable is that stainless steel flat plate is made, and is convenient to clean, and some supporting legs are installed under worktable, and the high 1cm of leg is unimpeded to ensure Bechtop air-flow, avoids forming miscellaneous bacteria dead angle.
On worktable 1, be fixed with culture dish anchor and test tube erecting frame to be inoculated, culture dish anchor wherein, for culture dish holding, in left hand station, is convenient to left-handed operation.Culture dish anchor comprises base 21, support cylinder 22, platform 23 and culture dish cover opening mechanism, base 21 is fixed by screws in left-handed operation one side of worktable 1, support cylinder 22 adopts stainless steel tube bending to form, and between support cylinder 22 and worktable, form one dodge below the space of test tube to be inoculated, make the state of cantilever.Be fixed on the upper end of support cylinder for the described platform of culture dish holding 9, platform is that stainless steel plate is made, and platform, support cylinder and base be all stainless steel, connects into as a whole by the form of welding.At a montant of horizontal bars welding of support cylinder 22, at the hinged lever 24 in the top of montant, the left end of this lever 24 freely, and can increase balancing weight at left end, on right-hand member, fix a sucker 25 that carries out adhesive with culture dish lid 91, the structure of sucker 25 is similar to existing sucker structure with pattern, and the sucker of this structure is often used in glass crawl, navigating instrument suction base.
Test tube erecting frame to be inoculated is arranged on right hand station, is convenient to the right hand and carries out manual operations.Particularly, described conveyer belt mechanism comprises conveying belt 31, live roll 32 and drive-motor 33, live roll 32 is two, tandem setting parallel to each other, be arranged on by bearing on the bearing base 34 being connected with worktable, more common in the mounting means of this structure after transmission shaft, when installation, the working face of described conveying belt 31 moves to far-end from operator's near-end, for considering the processing property of the right hand and observing superiority, by conveying belt together worktable be also fine in the side direction certain angle setting that tilts.Conveying belt is quality of rubber materials, and this conveying belt is often used in handling equipment.Drive-motor 33 is arranged on the below of worktable, can save space, uses belt transmission 35 between drive-motor and live roll, can drive the operation of conveying belt.On conveying belt 31, be provided with for the fixing test-tube bolder 36 of test tube to be inoculated, described test-tube bolder comprises flexure strip 361, arc-like sheet 362 and trip bolt 363, flexure strip 361 is for having unidirectional distortion function, its use from middle to thickness of two sides gradually the spring steel plate of thickening be made, and form two vertical picking up the ears in both sides by bending, two arc-like sheets 362 are welded on picking up the ears of flexure strip both sides, can make two arc-like sheets open by the slight swing flexure strip of external force, under normal circumstances, two arc-like sheets can form a circular space, for fixing test tube, there is rubber pad 364 in the inner side of arc-like sheet 362, can hold test tube tightly.Under the state opening at arc-like sheet, can make test tube come off.
At the front end of conveying belt 31, an inclined-plane 41 is set, the gradient is not too big, avoid causing tampon to come off and fragmentation in collision process, on inclined-plane 41, place the top layer with damping effect of string rubber or cotton, when test tube is after conveying belt front end turning point comes off, can roll down to the catch tray 42 in front along inclined-plane smoothly, test tube catch tray adopts stainless steel to make.
Above-mentioned flexure strip 361 is fixed on conveying belt by trip bolt, and uses in pairs, and the rear and front end that the test-tube bolder 36 using in pairs can be clamped test tube, forms two clamping parts, more firm.Hold intelligible, in the time that conveying belt forwards live roll place to, conveying belt is in steering state, now the flexure strip in test-tube bolder 36 can be out of shape along with conveying belt, and under the effect of external force, test-tube bolder 36 opens at conveying belt turning point, in the time that conveying belt forwards level attitude to, flexure strip in test-tube bolder recovers, and under this non-steering state, arc-like sheet closes up.
The below of process culture dish 9 in above-mentioned conveying belt operational process, and under culture dish, be provided with the sensor whether putting in place for detection of test tube 8 to be inoculated, in the time that sensor has detected test tube, motor stops action, can inoculate operation, complete when inoculating, left hand presses the button 7, conveying belt moves a station, is arranged on the left-hand side of worktable for controlling the button of drive-motor work, and operation more for convenience.
Inoculate for ease of the right hand, on the platform at culture dish place, weld a metallic bellows 6, this metallic bellows often uses in desk lamp, lathe lighting lamp, having can be bending and be positioned at the performance of certain position, and fix a clip 61 in one end of metallic bellows, can tampon 81 be clamped and be located, in the process of inoculation, can liberate the right hand, be convenient to the hand-held inoculating needle of the right hand and inoculate.
On worktable, be provided with a ring-type aseptic area centered by the test tube mouth of pipe to be inoculated being formed by some air outlets, and having at least an air outlet to face the test tube mouth of pipe to be inoculated place dries straight up, on air outlet, pass through the upwardly extending silica gel sheath 5 of buckle clamping one, the outlet of silica gel sheath is positioned at the downside of the test tube mouth of pipe to be inoculated, and exceed worktable 5 millimeter, blow out aseptic hot blast by upwards continual like this, make to inoculate region in sterile state, convenient inoculation.The existence of silica gel sheath can make the effective operation face of aseptic area be ensured, and ensures the Height Adjustable of air outlet by the silica gel sheath of changing different size.Install when test tube, under best state, culture dish 9 is got kind of a side, test tube to be inoculated 8 mouths of pipe all drop in aseptic area, makes in operating process all in the environment in aseptic technique.Can expect thus, 5-6 equally distributed air outlet need to be at least set on worktable, to form aseptic region.
Be provided with hot blast storehouse 51, heating cabinet 52 and air channel 53 at worktable downside, hot blast position in storehouse is in the downside of air outlet, and between air channel 53 and hot blast storehouse, heating cabinet 52 is set, and is provided with to the frequency conversion fan 54 of heating cabinet direction air blast in air channel.Heating cabinet 52 is designed to circle, in heating cabinet, be provided with the electro-heat equipment that can produce high temperature, this kind of heating unit can adopt electrical heating element, this element often uses in hot hair dryer, for the resistance wire of spirrillum or plate-like, under heated condition, it can generate heat and redden, and the temperature of nucleus is up to several Baidu.On worktable corresponding to electro-heat equipment, be provided with the pin hole 55 of a up/down perforation, the needle body of one inoculating needle 71 is positioned at the heating core area of heating unit just through pin hole, to make full use of the high temperature of Heating element, tip portion to inoculating needle heats, and then realize sterilization, above-mentioned pin hole is in the right-hand operated side of worktable.
Above-mentioned pin hole is this structure: in electro-heat equipment heating core area, right workplatform is provided with a boring, above boring correspondence, fixes a copper sheathing, has one and the coaxial tapered hole arranging of boring in described copper sheathing.
In the utility model, conveying belt 31 is for placing test tube to be inoculated, and utilize small power drive-motor 33 that power is provided, by push-button control rotate and put in place after automatic pause, the position of each test tube of feeding, on conveying belt, be fixed with somely to test-tube bolder, test-tube bolder can automatically unclamp while moving to upset place test tube is come off carrying under driving, and can complete the function of manual fixing test tube and automatic discharging.Culture dish bracing frame is for fixing culture dish and be provided with the on-off structure that unlatching culture dish is somebody's turn to do thereon, adopts lever principle to carry out opening and closing to lid, and a finger can operate.On the platform at culture dish place, install can free adjustment angle metallic bellows, and at the free end of metallic bellows, clip is set, can clamp the tampon of test tube, and tampon is positioned to any position, without hand-held.
Funnel-shaped blowing mouth, according to the design of airflow pattern feature, is installed by aseptic blowing district in bottom, the outlet of silica gel sheath is apart from the high 5cm of hot spot plane, and blast inlet is in inner side, to accept the air that aseptic operating platform blows; Annular heating zone is installed, apart from blowing mouth pipeline 2cm, hot zone width 1cm, in-built electrical heated filament in bottom panel.Blowing amount and power of heating wire are all adjustable.One, inoculating needle sterilization hole is separately set, can carries out disinfection to inoculating needle, and can provide clean sterile air for aseptic area.
When operation, first open electrically heated, blowing, on device, test tube to be inoculated and culture dish are installed, by inoculating needle sterilization in sterilization hole, bottom, will test tube be inoculated move near culture dish transfers, open ware lid with ware lid support, make ware lid get bacterium piece one side and open approximately 0.5 gap to 1.0cm, with inoculating needle picking bacterium piece or mycelia, take automatically and close, then with test tube tampon to be inoculated under tampon gripping, bacterium piece or mycelia are moved on test tube slant to be inoculated, cover tampon.Again mobile next only test tube to be inoculated near culture dish, repeat aforesaid operations.
Embodiment recited above is described preferred implementation of the present utility model; not scope of the present utility model is limited; do not departing under the prerequisite of the utility model design spirit; relevant technical staff in the field, to various distortion of the present utility model and improvement, all should expand in protection domain as determined in the utility model claims.

Claims (6)

1. a batch classification inoculation apparatus for automatization, comprises worktable, it is characterized in that:
On described worktable, be fixed with culture dish anchor and test tube erecting frame to be inoculated, described culture dish anchor comprises base, support cylinder, platform and culture dish cover opening mechanism, described base through screws is fixed on left-handed operation one side of worktable, described support cylinder upwards extends and forms a space of dodging below test tube to be inoculated, be fixed on the upper end of support cylinder for the described platform of culture dish holding, a hinged lever on described support cylinder, one end of described lever is provided with a sucker that carries out adhesive with culture dish lid; On described platform, install can free adjustment angle metallic bellows, and clip that can holding test tubes tampon in the free end setting of metallic bellows,
Fix a horizontality conveyer belt mechanism along worktable fore-and-aft direction, described conveyer belt mechanism comprises conveying belt, live roll and drive-motor, the working face of described conveying belt moves to far-end from operator's near-end, and on described conveying belt, be provided with for the fixing test-tube bolder of test tube to be inoculated, described test-tube bolder opens at conveying belt turning point, close up at non-turning point, described drive-motor drives described live roll to rotate, and described conveying belt is through the below of culture dish; And under culture dish, be provided with the sensor whether putting in place for detection of test tube to be inoculated, be arranged on the left-hand side of worktable for controlling the button of drive-motor work, on the worktable of described conveying belt front end, establish a collecting mechanism of collecting test tube,
On described worktable, be provided with a ring-type aseptic area centered by the test tube mouth of pipe to be inoculated being made up of some air outlets, on air outlet, by the upwardly extending silica gel sheath of buckle clamping one, the outlet of described silica gel sheath is positioned at the downside of the test tube mouth of pipe to be inoculated,
When test tube is installed, described culture dish is got kind of a side, the test tube mouth of pipe to be inoculated is positioned at the aseptic area that air outlet top forms,
Be provided with hot blast storehouse at described worktable downside, heating cabinet and air channel, described hot blast position in storehouse is in the downside of air outlet, and between air channel and hot blast storehouse, heating cabinet is set, described air channel is communicated with heating cabinet and air suction inlet, and in air channel, be provided with to the frequency conversion fan of heating cabinet direction air blast, in described heating cabinet, be provided with the electro-heat equipment that can produce high temperature, and on worktable corresponding to described electro-heat equipment, be provided with the pin hole of a up/down perforation, the needle body of one inoculating needle is positioned at the heating core area of heating unit just through pin hole, described pin hole is in the right-hand operated side of worktable.
2. the batch classification inoculation apparatus of a kind of automatization according to claim 1, it is characterized in that, described test-tube bolder comprises flexure strip, arc-like sheet and trip bolt, described flexure strip is have unidirectional distortion function certainly middle to the thickness of two sides spring steel plate of thickening gradually, described spring steel plate is fixed on conveying belt, described arc-like sheet can form the both sides that are fixed on flexure strip of a circular space, and has rubber pad in the inner side of arc-like sheet.
3. the batch classification inoculation apparatus of a kind of automatization according to claim 1, it is characterized in that, described pin hole is this structure: in electro-heat equipment heating core area, right workplatform is provided with a boring, above boring correspondence, fix a copper sheathing, in described copper sheathing, there is one and the coaxial tapered hole arranging of boring.
4. the batch classification inoculation apparatus of a kind of automatization according to claim 1, it is characterized in that, described collecting mechanism comprises inclined-plane and catch tray, described catch tray is fixed on worktable, and the two ends on inclined-plane are connected on the front end and catch tray of conveying belt, and inclined-plane has the top layer of damping effect.
5. the batch classification inoculation apparatus of a kind of automatization according to claim 1, is characterized in that, described electro-heat equipment is discoid or spiral helicine resistance wire.
6. the batch classification inoculation apparatus of a kind of automatization according to claim 1, is characterized in that, is provided with one and faces the air outlet of drying straight up in the test tube mouth of pipe to be inoculated place on described worktable.
CN201420202630.2U 2014-04-24 2014-04-24 Automatic batch inoculation device Withdrawn - After Issue CN203807471U (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103952298A (en) * 2014-04-24 2014-07-30 山东省农业科学院玉米研究所 Device used for batch inoculation in aseptic operation
CN104498328A (en) * 2014-12-18 2015-04-08 重庆康乐制药有限公司 Test tube plug clamping device used in inoculation box
CN104560694A (en) * 2015-01-30 2015-04-29 重庆道鑫生物科技有限公司 Microbiological culture medium device and operating method thereof
CN106281992A (en) * 2015-05-18 2017-01-04 北京慧荣和科技有限公司 Full-automatic Ames test instrument

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103952298A (en) * 2014-04-24 2014-07-30 山东省农业科学院玉米研究所 Device used for batch inoculation in aseptic operation
CN104498328A (en) * 2014-12-18 2015-04-08 重庆康乐制药有限公司 Test tube plug clamping device used in inoculation box
CN104560694A (en) * 2015-01-30 2015-04-29 重庆道鑫生物科技有限公司 Microbiological culture medium device and operating method thereof
CN106281992A (en) * 2015-05-18 2017-01-04 北京慧荣和科技有限公司 Full-automatic Ames test instrument
CN106281992B (en) * 2015-05-18 2024-02-09 北京慧荣和科技有限公司 Full-automatic Ames experiment instrument

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