CN205803456U - Cell scratch experiment six orifice plates - Google Patents
Cell scratch experiment six orifice plates Download PDFInfo
- Publication number
- CN205803456U CN205803456U CN201620744530.1U CN201620744530U CN205803456U CN 205803456 U CN205803456 U CN 205803456U CN 201620744530 U CN201620744530 U CN 201620744530U CN 205803456 U CN205803456 U CN 205803456U
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- CN
- China
- Prior art keywords
- cover plate
- orifice plates
- sliding cover
- scratch experiment
- hollow out
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Abstract
This utility model provides a kind of cell scratch experiment six orifice plates: include six hole slots and double-layer cover plate structure, described double-layer cover plate structure includes securing cover plate and the sliding cover plate that stacking arranges, described securing cover plate and six hole slots are connected, region relative with six hole slots on described securing cover plate is provided with several hollow out bar-shaped troughs, described sliding cover plate can slide by the most described securing cover plate, and described hollow out bar-shaped trough is by described sliding cover closure.This utility model, by using double-layer cover plate structure, had both remained marking groove, solved again the closed question of six orifice plates after scoring operation, effectively prevent the living contaminants that cultivation stage occurs, simple and reliable for structure, easy to use.By making cut pin move with sliding cover plate, the efficiency of slide mark experiment can be improved further.
Description
Technical field
This utility model belongs to Biotechnology Experiment equipment, relates to six orifice plates, is specifically related to and the need of cell scratch experiment
Seek six orifice plates adapted.
Background technology
Along with tumor problem of being correlated with increasingly is favored by researcher, the related pathologies characteristic of research tumor is the most just
Become a most necessary link.During probing into tumor characteristic, transfer ability is as probing into tumor cell behavior
The important component part learned is by the extensive attention of researcher.Many methods in checking tumor cell migration ability
In, as a basis, important experiment becomes the major criterion judging cell migration ability to scratch experiment.
At present, the way of general scratch experiment is to prepare one or six orifice plates, and in using after cell monolayer is paved with, rifle head is in six holes
On plate, cell laying is rule.After culture medium culturing a period of time of serum-free, the migration situation of observation of cell.
Most of researchers are usually and by virtue of experience carry out manual line.But, for the spacing of line and line
Holding, line dynamics and the control of straight degree of line are the most not fully up to expectations.Therefore, reduce experimental result reliability and
Accuracy.
By retrieving current existing solution, although utilize reusable marking groove as " ruler " of line
Can ensure that the straight and convenient of line to a certain degree, but ignoring marking groove and bring extraneous antibacterial into nothing that cell is cultivated
Bacterium space, lacks the technological means closing marking groove, it is impossible to reach the requirement of aseptic culture.
Summary of the invention
The purpose of this utility model is to provide a kind of cell scratch experiment six orifice plates.
For reaching above-mentioned purpose, this utility model have employed techniques below scheme:
Including six hole slots and double-layer cover plate structure, described double-layer cover plate structure include securing cover plate that stacking arranges and
Sliding cover plate, described securing cover plate and six hole slots are connected, and region relative with six hole slots on described securing cover plate sets respectively
Being equipped with several hollow out bar-shaped troughs, described sliding cover plate can slide by the most described securing cover plate, and described hollow out bar-shaped trough passes through institute
State sliding cover closure.
Described double-layer cover plate structure also include being arranged on described securing cover plate for the cunning as described sliding cover plate
The slide groove of dynamic track.
The scale for identifying described hollow out bar-shaped trough length it is provided with on described securing cover plate.
The cut direction that the glide direction of described sliding cover plate retrains with described hollow out bar-shaped trough is parallel.
It is provided with the installing hole overlapped with described hollow out bar-shaped trough position on described sliding cover plate, in installing hole, is provided with use
In the rubber stopper of fixing cut pin, described cut pin includes the shank being connected with described rubber stopper and is arranged at described shank end
The cut head in portion.
Described hole slot and double-layer cover plate structure use hard transparent material to make.
It is provided with handle on described sliding cover plate.
The beneficial effects of the utility model are embodied in:
This utility model, by using double-layer cover plate structure, had both remained marking groove, had solved again six holes after scoring operation
The closed question of plate, effectively prevent the living contaminants that cultivation stage occurs, simple and reliable for structure, easy to use.
Accompanying drawing explanation
Fig. 1 is front view of the present utility model；
Fig. 2 is side view of the present utility model；
Fig. 3 is the scheme of installation (vertical view) of sliding cover plate；
Fig. 4 is the distribution schematic diagram of installing hole；
Fig. 5 is the scheme of installation of cut pin；
In figure: 1 is six orifice plate bodies, and 11 is hole slot, and 12 is base plate, and 2 is securing cover plate, 3 is sliding cover plate, and 4 is slideway
Groove, 5 is hollow out bar-shaped trough, and 6 is installing hole, and 7 is rubber stopper, and 8 is cut pin, and 9 is scale.
Detailed description of the invention
With embodiment, this utility model is elaborated below in conjunction with the accompanying drawings.
The lid of six orifice plates is reequiped by this utility model, is allowed to adapt to experiment demand, accomplishes both to prevent cell dirty
Dye, can mark again the most neat cut, improve scratch experiment efficiency simultaneously.
See Fig. 1, Fig. 2 and Fig. 3, the six AND DEWATERING FOR ORIFICE STRUCTURE features for cell scratch experiment provided by the utility model
As follows:
Six orifice plates are still made up of two parts, are six orifice plate body 1 and lids respectively, and material is transparent plastic or organic glass
Glass.Six orifice plate bodies 1 mainly include base plate 12 and six hole slots 11 according to two row three column matrix formation arrangements.This practicality is new
Type is science and the aseptic of operation ensureing experiment to the full extent, only transforms lid accordingly.Improved
Lid includes securing cover plate 2 and the sliding cover plate 3 that stacking arranges, and securing cover plate 2 is covered in hole slot 11 upper end open, fixing lid
According to diameter and experiment demand processing hollow type rifle head dragging track (i.e. hollow out bar-shaped trough 5), the described rifle head of middle rifle head on plate 2
Dragging track Working position is relative with hole slot 11, and described rifle head dragging track both for moving into cell in hole slot 11, was used as again
The marking-out rule tool of rifle head in cut.On securing cover plate 2 dual-side edge, (parallel with described rifle head dragging track) rational height is 1-
The slide groove 4 of 2mm, the opening of groove is relative, for forming the sliding rail of sliding cover plate 3, is not only determined sliding cover plate 3
Position, and make sliding cover plate 3 can be relatively fixed cover plate 2 and slide (glide direction is identical with cut direction or contrary).
Six orifice plates (covering described rifle head dragging track) are covered by slip sliding cover plate 3 after scratch experiment, anti-
Only germ contamination.Sliding cover plate 3 both sides arrange little short handle, facilitate while taking out sliding shifting cover plate 3, ensure to greatest extent
Sterile working.
Above-mentioned six orifice plates for cell scratch experiment have the advantage that
1. by scientifically formulating the distance of the size of hollow out bar-shaped trough and groove and groove, can make cut straight degree and
Distance between cut reaches optimization so that the cell migration result of acquisition has more science and cogency.
2. utilizing the six original architecture basics of orifice plate lid to improve, systematic error is less, will not former because of equipment
Because affecting experimental result.
3. the lid of six orifice plates belongs to disposable sterilized equipment, and on this basis, the transformation that this utility model is carried out ensures
Test on the premise of normal sterile operation and be smoothed out, it also avoid other relevant design and do not focus on the drawback of sterile working.
4. newly-increased sliding cover plate can make cell and extraneous antibacterial isolation, it is ensured that cultivation stage is aseptic.
5. simple in construction, application potential is bigger.
Change the most on the original basis is the most novel, and function is more targeted.Material expense of increasing input is less.
In order to can complete for the scoring operation in multiple hole slots the most simultaneously, this utility model is at above-mentioned sliding cover plate
It is further improved on 3.See Fig. 4 and Fig. 5, on sliding cover plate 3, i.e. offer installing hole 6, by rubber stopper 7 in hole
Fixing cut pin 8.The position of installing hole 6 overlaps with the position of hollow type rifle head dragging track, is usually located at this track two side ends
The original position of the position in portion, i.e. cut.Cut pin 8 body sections (shank) is fixed on sliding cover plate 3 by rubber stopper 7, no
Only can keep good sealing effectiveness, nor hinder and carry out cut pin 8 lifting displacement operation, the head of cut pin 8 is used
In cut, size is more than body sections diameter, equivalently-sized or similar to middle rifle head front end.Sliding cover plate 3 is pushed slide groove
4, until installing hole 6 reaches corresponding hollow type rifle head dragging track one end, preprepared cut pin 8 is loaded installing hole 6
And (through hollow type rifle head dragging track) is pushed into bottom hole slot 11 downwards, makes cut pin 8 head and cultivate cells contacting, then
Be pushed further into sliding cover plate 3, until cut pin 8 reach the corresponding hollow type rifle head dragging track other end or determine position (according to
Scale 9 on securing cover plate 3 judges).So, not only can complete to organize scoring operation more simultaneously, and cut terminates to close
Six orifice plates, it is thus possible to improve scratch experiment efficiency, and reduce microbiological contamination risk further.
Claims (7)
1. cell scratch experiment six orifice plates, it is characterised in that: include six hole slots (11) and double-layer cover plate structure, institute
State securing cover plate (2) and sliding cover plate (3) that double-layer cover plate structure includes that stacking arranges, described securing cover plate (3) and six holes
Groove (11) is connected, and the upper region relative with six hole slots (11) of described securing cover plate (2) is respectively arranged with several hollow out bar shapeds
Groove (5), described sliding cover plate (3) can the most described securing cover plate (2) slide, described hollow out bar-shaped trough (5) is by described sliding
Cover plate (3) is closed.
A kind of cell scratch experiment six orifice plates, it is characterised in that: described double-layer cover plate structure is also
Including the slide groove (4) for the sliding rail as described sliding cover plate (3) being arranged on described securing cover plate (2).
A kind of cell scratch experiment six orifice plates, it is characterised in that: on described securing cover plate (2)
It is provided with the scale (9) for identifying described hollow out bar-shaped trough (5) length.
A kind of cell scratch experiment six orifice plates, it is characterised in that: described sliding cover plate (3)
The cut direction that glide direction retrains with described hollow out bar-shaped trough (5) is parallel.
A kind of cell scratch experiment six orifice plates, it is characterised in that: on described sliding cover plate (3)
It is provided with the installing hole (6) overlapped with described hollow out bar-shaped trough (5) position, is provided with for fixing cut pin in installing hole (6)
(8) rubber stopper (7), described cut pin (8) includes the shank being connected with described rubber stopper (7) and is arranged at described shank end
The cut head in portion.
A kind of cell scratch experiment six orifice plates, it is characterised in that: described hole slot (11) and double
Layer covering plate structure uses hard transparent material to make.
A kind of cell scratch experiment six orifice plates, it is characterised in that: on described sliding cover plate (3)
It is provided with handle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620744530.1U CN205803456U (en) | 2016-07-14 | 2016-07-14 | Cell scratch experiment six orifice plates |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620744530.1U CN205803456U (en) | 2016-07-14 | 2016-07-14 | Cell scratch experiment six orifice plates |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205803456U true CN205803456U (en) | 2016-12-14 |
Family
ID=57511549
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620744530.1U Expired - Fee Related CN205803456U (en) | 2016-07-14 | 2016-07-14 | Cell scratch experiment six orifice plates |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205803456U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106520525A (en) * | 2017-01-01 | 2017-03-22 | 重庆科技学院 | Cell scratch test cover |
| CN106701534A (en) * | 2017-01-01 | 2017-05-24 | 重庆科技学院 | Application method of cell wound scratch assay cover |
-
2016
- 2016-07-14 CN CN201620744530.1U patent/CN205803456U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106520525A (en) * | 2017-01-01 | 2017-03-22 | 重庆科技学院 | Cell scratch test cover |
| CN106701534A (en) * | 2017-01-01 | 2017-05-24 | 重庆科技学院 | Application method of cell wound scratch assay cover |
| CN106520525B (en) * | 2017-01-01 | 2019-03-05 | 重庆科技学院 | A kind of cell scratch experiment lid |
| CN106701534B (en) * | 2017-01-01 | 2019-04-05 | 重庆科技学院 | A kind of application method of cell scratch experiment lid |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20161214 Termination date: 20170714 |