CN106701534A - Application method of cell wound scratch assay cover - Google Patents
Application method of cell wound scratch assay cover Download PDFInfo
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- CN106701534A CN106701534A CN201710000016.6A CN201710000016A CN106701534A CN 106701534 A CN106701534 A CN 106701534A CN 201710000016 A CN201710000016 A CN 201710000016A CN 106701534 A CN106701534 A CN 106701534A
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- lid
- cut
- cover
- cuboid
- culture dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
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Abstract
The invention provides an application method of a cell wound scratch assay cover. The application method is characterized in that a used scratch cover is one cuboid-shaped bridge-shaped cover, at the cover-free edges of the two long sides of a cuboid, circular-arc cover edges are arranged at the two ends of the long sides of the cuboid, and the cover edges can be buckled on the circular-arc outer side wall of a cell culture dish; a main cover comprises a cover edge at one end of a scratch cover and a cuboid cover, and the cover edge at the other end of the scratch cover is an independent and separate movable clamping cover; and the end plane of the main cover of the scratch cover and the movable clamping cover are in an external-force-free state and fit closely under the action of an elastic tension device. The application method provided by the invention comprises: mounting, inserting a needle, scratching, and taking out the needle. The application method provided by the invention has the beneficial effects and technical characteristics of convenience and simplicity in production, low cost, strong scratch identity, more accurate contrast experimental data and simple and easy operation.
Description
Technical field
The present invention relates to a kind of application method of the small device for cell experiment, further particularly say, be a kind of life
The application method of thing engineering and the cell cut lid of medical science aspect cell growth migration experiment.
Background technology
Tumor tissue, the proliferation out of control of cancerous tissue, the disdifferentiation of oncocyte, oncocyte are with invasion and attack and the ability for shifting
The most basic biological property of malignant tumour, and attack and transfer be malignant tumour threat patient health so that life it is main
Reason.Therefore the rule and its mechanism of invasion and metastasis of tumor are studied, the preventing and treating to malignant tumour is significant.Cell
Scarification is one of the method for kinetic characteristic for determining tumour cell.Its use for reference the proliferation out of control of tumor tissue, oncocyte point
Change exception, knurl, cancer cell to have the ability of invasion and attack and transfer is that malignant tumour most basal cell's scarification is that simple and direct measure cell is moved
Shifting movement and the method for repair ability, are similar to external wound healing model, in vitro culture dish or the monolayer adherence of flat board culture
On cell, with micro pipette tips or other hard things in the middle section cut of cell growth, the cell of middle body, Ran Houji are removed
Continuous cultured cells takes out Tissue Culture Plate to the time for testing setting, and whether observation periphery cell grows reparation to central cut
Area, the growth transfer ability of cell is judged with this.Experiment need to generally set Normal group and experimental group, and experimental group is to have added certain
Plant the group for the treatment of factor or medicine, allogenic gene etc., the reparation energy by the cell between different grouping for cut area
Power, it can be determined that the migration of each group cell and repair ability.
However, regular growth scratch experiment is all to complete the cut of cell than ruler with pipette tips, pipette tips requirement is vertical,
Can not incline, but, in operation, due to each gimmick error, cause the vestige width for marking to completely unify, contrast
The effect of experiment can not be very accurate.Cell scratch experiment needs a kind of specialty, marks the consistent experimental provision of width size.
Accordingly, it would be desirable to the scoring devices and application method of a kind of cell growth observation of specialty, for bioengineering and
In the cell scratch experiment of medical research.
The content of the invention
In order to solve the above problems, the technical scheme is that：A kind of application method of cell scratch experiment lid, uses
Cell scratch experiment cut lid, be mainly made up of main cover and movable clamping lid two parts.It is characterized in that：Cut lid is in one
The bridge like lid of individual cuboid, cuboid two it is long while without lid along while, there is the lid of circular arc at the side two ends long of cuboid
Son can be buckled on the circular arc lateral wall of Tissue Culture Dish along side.The main cover includes the lid of cut lid one end along side and length
Cube lid, the lid of the other end of cut lid is the movable clamping lid of independent separate along side；In the cuboid lid face of main cover
On be machined with 1 or 2 sliding-rail groove be parallel to each other, insertion cuboid lid face, be provided with being capable of edge for processing on sliding-rail groove
The crosshead shoe of sliding-rail groove planar movement.In the cuboid lid body of main cover, near movable clamping lid side it is rectangular in
On axis, tensioning chamber is machined with, tensioning is provided with elastic stretching device inside chamber.Elastic stretching device is in natural shrinking such as bullet
Spring etc., will add external force when pulling open, after removal external force, original state be shunk back again.It is tensioned the cuboid lid body of the parallel both sides in chamber
Inside it is machined with 2 flat chambers of inserted sheet；Tensioning chamber and 2 flat chambers of inserted sheet are all machined with opening on the end face towards movable clamping lid.Institute
State movable clamping to cover on correspondence tensioning chamber and 2 positions of the flat chamber opening of inserted sheet, processing is fixed with 1 elastic threads and 2 straight
Inserted sheet is fixed at angle.Elastic threads are fixedly connected by the elastic stretching device for being tensioned chamber opening be tensioned inside chamber.Purpose of design makes
Movable clamping lid can be brought into close contact with main cover all the time.2 right angles fix that inserted sheets are inserted into or 2 inserted sheets corresponding to extracting are flat
Chamber.Without external force state, in the presence of elastic stretching device, Flat-end and the movable clamping of the main cover of cut lid cover tightly close phase
Laminating.The design of inserted sheet is fixed at right angle, has been ensured under the active force of tensioning apparatus, and movable clamping lid all the time can be with the end of main cover
Face, is at right angles brought into close contact, and does not misplace, not on turn over.To ensure that cell scratch experiment lid can be brought into close contact Tissue Culture Dish
Outer wall.The application method of a kind of cell cut lid of above-mentioned technical proposal, it is characterised in that operating procedure is as follows.
The first step, installs.The cut lid with the Tissue Culture Dish units specification of experiment is chosen, Tissue Culture Dish is removed
Lid, unplugs movable clamping lid, by cut cover buckle in Tissue Culture Dish.
Second step, contact pin.After fixed clamping cut lid, crosshead shoe is moved on to the top of sliding-rail groove one end, choose experiment
It is required that diameter cut pin, insert crosshead shoe pin holes in, adjustment height, to cut pin pass through Tissue Culture Dish
The cellular layer of bottom is until Tissue Culture Dish bottom, clamping fixation cut pin.
3rd step, cut.Pin crosshead shoe and at the uniform velocity slide into other end top from the top of sliding-rail groove, complete cell and draw
Trace.If main cover is that have two sliding-rail grooves, second operation of sliding-rail groove repeats second step and the 3rd step.
4th step, takes out cut pin, unplugs movable clamping lid, removes and removes cut lid, covers the lid of Tissue Culture Dish.
A kind of application method of the cell cut lid described in above-mentioned technical proposal, it is characterised in that the crosshead shoe for using
Orthogonal cross-shaped in two pieces of flat cuboids, one piece of prolate cube can be embedded in sliding-rail groove and slide, vertical
The cuboid lid face smooth contact of another piece of prolate cube and main cover.Design secures crosshead shoe, and hand push ten is facilitated again
Word sliding block.The pin holes of up/down perforation sliding block are machined with the crossing center of crosshead shoe, pin holes can install cut pin, drawn
The respectively different thickness specification of trace pin tip diameter.This design is to be convenient for changing cut pin.The specification and cell of cut lid
Culture dish standard size is supporting, in order to support the use.
The technology of the present invention feature is simple and convenient and has professional.The present invention has following beneficial effect：（1）It is easy to make
Simply, it is with low cost.（2）Cut homogeneity is strong, and comparative experimental data is more accurate.（3）It is simple, convenient.
Brief description of the drawings
Fig. 1 is the half-sectional schematic front view assembled and use of the invention.
Fig. 2 is the schematic top plan view that present invention figure assembling is used.
Fig. 3 is A-A schematic cross-sectional views of the invention.
Fig. 4 is crosshead shoe schematic top plan view of the invention.
Fig. 5 is the schematic front view that crosshead shoe of the invention installs scriber.
In figure：1. cut lid；2. main cover；3. crosshead shoe；4. cut pin；5. chamber is tensioned；6. elastic stretching device；7. bullet
Property rope；8. movable clamping lid；9. Tissue Culture Dish；10. cellular layer；The flat chamber of 11. inserted sheets；Inserted sheet is fixed at 12. right angles；13. slide rails
Groove；14. pin holes.
Specific embodiment
Further the present invention is illustrated with reference to the accompanying drawings and examples.
Embodiment one
Referring to figs. 1 to the shape and structure of Fig. 5, a kind of cell scratch experiment lid and application method, the specification of cut lid 1 is：Most greatly enhance
Degree, has the diameter=100mm of circular arc equivalent to the side two ends long of the cuboid of cut lid 1, there is 2 sliding-rail grooves being parallel to each other
13.The Tissue Culture Dish for being suitable for diameter 100mm is used.It is as follows using operating procedure.
The first step, removes the lid of the Tissue Culture Dish 9 of diameter 100mm, unplugs movable clamping lid 8, and cut lid 1 is buckled in
Tissue Culture Dish 9.
Second step, after fixed clamping cut lid 1, crosshead shoe 3 is moved on to the top of the one end of sliding-rail groove 13, chooses diameter
The cut pin 4 of=2mm, is inserted in the pin holes 14 of crosshead shoe 3, adjustment height, and the bottom of Tissue Culture Dish 9 is passed through to cut pin 4
Cellular layer 10 until the bottom of Tissue Culture Dish 9.Cut pin 4 is fixed in clamping.
3rd step, pins crosshead shoe 3, the prolate cube vertical with sliding-rail groove 13 of crosshead shoe 3 is at the uniform velocity pushed away, from sliding-rail groove
13 top slides into other end top, completes cell cut.Then, cut pin 4, on insertion Article 2 sliding-rail groove 13 ten are extracted
In the pin holes 14 of word sliding block 3, second step and the operation of the 3rd step are repeated.
4th step, takes out cut pin 4, unplugs movable clamping lid 8, removes and removes cut lid 1, covers Tissue Culture Dish 9
Lid.
Embodiment two
Referring to figs. 1 to the shape and structure of Fig. 5, a kind of cell scratch experiment lid and application method, the specification of cut lid 1 is：Most greatly enhance
Degree, has the diameter=60mm of circular arc equivalent to the side two ends long of the cuboid of cut lid 1, there is 2 sliding-rail grooves being parallel to each other
13.Use the cut pin 4 of diameter=1.5mm.The Tissue Culture Dish for being suitable for diameter 60mm is used.Operation is with reference to embodiment
One.
Embodiment three
Referring to figs. 1 to the shape and structure of Fig. 5, a kind of cell scratch experiment lid and application method, the specification of cut lid 1 is：Most greatly enhance
Degree, has the diameter=35mm of circular arc equivalent to the side two ends long of the cuboid of cut lid 1, there is 1 sliding-rail groove being parallel to each other
13.The Tissue Culture Dish for being suitable for diameter 35mm is used.It is as follows using operating procedure.
The first step, removes the lid of the Tissue Culture Dish 9 of diameter 35mm, unplugs movable clamping lid 8, and cut lid 1 is buckled in
Tissue Culture Dish 9.
Second step, after fixed clamping cut lid 1, crosshead shoe 3 is moved on to the top of the one end of sliding-rail groove 13, chooses diameter
The cut pin 4 of=1mm, is inserted in the pin holes 14 of crosshead shoe 3, adjustment height, and the bottom of Tissue Culture Dish 9 is passed through to cut pin 4
Cellular layer 10 until the bottom of Tissue Culture Dish 9.Cut pin 4 is fixed in clamping.
3rd step, pins crosshead shoe 3, the prolate cube vertical with sliding-rail groove 13 of crosshead shoe 3 is at the uniform velocity pushed away, from sliding-rail groove
13 top slides into other end top, completes cell cut.
4th step, takes out cut pin 4, unplugs movable clamping lid 8, removes and removes cut lid 1, covers Tissue Culture Dish 9
Lid.
Claims (2)
1. a kind of application method of cell scratch experiment lid, the cut lid of the cell scratch experiment for using（1）Mainly by main cover（2）
With movable clamping lid（8）Two parts are constituted；Cut lid（1）In a bridge like lid for cuboid, the side long of cuboid two without
Lid along side, cuboid it is long while two ends have the lid of circular arc along while, the circular arc lateral wall of Tissue Culture Dish can be buckled in
On；The main cover（2）Including cut lid（1）The lid of one end is along side and cuboid lid, cut lid（1）The other end lid
It is the movable clamping lid of independent separate along side（8）；In main cover（2）Cuboid lid face on be machined with 1 or 2 it is mutually flat
The sliding-rail groove in capable, insertion cuboid lid face（13）, sliding-rail groove（13）Upper processing is provided with can be along sliding-rail groove（13）Plane
Mobile crosshead shoe（3）；In main cover（2）In cuboid lid body, near movable clamping lid（8）The rectangular axis in side
On, it is machined with tensioning chamber（5）, it is tensioned chamber（5）Inside is provided with elastic stretching device（6）；Tensioning chamber（5）Parallel both sides it is rectangular
2 flat chambers of inserted sheet are machined with body lid body（11）；Tensioning chamber（5）With 2 flat chambers of inserted sheet（11）All towards movable clamping lid（8）
End face on be machined with opening；The movable clamping lid（8）In correspondence tensioning chamber（5）With 2 flat chambers of inserted sheet（11）The position of opening
Put, processing is fixed with 1 elastic threads（7）Inserted sheet is fixed with 2 right angles（12）；Elastic threads（7）By being tensioned chamber（5）Opening with
Tensioning chamber（5）Internal elastic stretching device（6）It is fixedly connected；Inserted sheet is fixed at 2 right angles（12）It is inserted into or extracts institute right
The 2 flat chambers of inserted sheet answered（11）；Without external force state, in elastic stretching device（6）In the presence of, cut lid（1）Main cover（2）'s
Flat-end and movable clamping lid（8）Closely fit；Characterized in that, operating procedure is as follows：
The first step, installs, and chooses the Tissue Culture Dish with experiment（9）The cut lid of units specification（1）, remove Tissue Culture Dish
（9）Lid, unplugs movable clamping lid（8）, by cut lid（1）It is buckled in Tissue Culture Dish（9）；
Second step, contact pin, fixed clamping cut lid（1）Afterwards, by crosshead shoe（3）Move on to sliding-rail groove（13）The top of one end, choosing
Take the cut pin of the diameter of requirement of experiment（4）, insert crosshead shoe（3）Pin holes（14）It is interior, adjustment height, to cut
Pin（4）Through Tissue Culture Dish（9）The cellular layer of bottom（10）Until Tissue Culture Dish（9）Cut pin is fixed in bottom, clamping
（4）；
3rd step, cut pins crosshead shoe（3）At the uniform velocity from sliding-rail groove（13）Top slide into the other end top, complete cell
Cut；If any second sliding-rail groove（13）, operation repetition second step and the 3rd step；
4th step, takes out cut pin（4）, unplug movable clamping lid（8）, remove and remove cut lid（1）, cover Tissue Culture Dish
（9）Lid.
2. the application method of a kind of cell scratch experiment lid according to claim 1, it is characterised in that：The cross for using is slided
Block（3）Orthogonal cross-shaped in two pieces of flat cuboids, one piece of prolate cube can be embedded in sliding-rail groove（13）Interior cunning
It is dynamic, another piece of vertical prolate cube and main cover（2）Cuboid lid face smooth contact；In crosshead shoe（3）Intersection in
The heart is machined with the pin holes of up/down perforation sliding block（14）, pin holes（14）Cut pin can be installed（4）, cut pin（4）Termination is straight
The respectively different thickness specification in footpath；Cut lid（1）Specification and Tissue Culture Dish（9）Standard size is supporting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710000016.6A CN106701534B (en) | 2017-01-01 | 2017-01-01 | A kind of application method of cell scratch experiment lid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710000016.6A CN106701534B (en) | 2017-01-01 | 2017-01-01 | A kind of application method of cell scratch experiment lid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106701534A true CN106701534A (en) | 2017-05-24 |
| CN106701534B CN106701534B (en) | 2019-04-05 |
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| CN201710000016.6A Active CN106701534B (en) | 2017-01-01 | 2017-01-01 | A kind of application method of cell scratch experiment lid |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1596344A (en) * | 2001-11-27 | 2005-03-16 | 赫伯特·汉切恩两合公司 | Clamping device and regulating unit |
| US20110195489A1 (en) * | 2010-02-08 | 2011-08-11 | Rongpei Lan | System and Method of an In-vitro Wound Healing Model on Cultured Cell Monolayer Employing Stamp Device |
| CN203728831U (en) * | 2014-03-19 | 2014-07-23 | 中南大学湘雅医院 | Cell scratch experiment device |
| CN204550528U (en) * | 2015-03-26 | 2015-08-12 | 中国人民解放军第四军医大学 | A kind of cell scuffing experimental device |
| CN205774533U (en) * | 2016-05-24 | 2016-12-07 | 黄嘉炜 | A kind of cell scratch experiment incubator |
| CN205803456U (en) * | 2016-07-14 | 2016-12-14 | 李子超 | Cell scratch experiment six orifice plates |
| CN206385125U (en) * | 2017-01-01 | 2017-08-08 | 重庆科技学院 | Cell scratch experiment cut lid |
-
2017
- 2017-01-01 CN CN201710000016.6A patent/CN106701534B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1596344A (en) * | 2001-11-27 | 2005-03-16 | 赫伯特·汉切恩两合公司 | Clamping device and regulating unit |
| US20110195489A1 (en) * | 2010-02-08 | 2011-08-11 | Rongpei Lan | System and Method of an In-vitro Wound Healing Model on Cultured Cell Monolayer Employing Stamp Device |
| CN203728831U (en) * | 2014-03-19 | 2014-07-23 | 中南大学湘雅医院 | Cell scratch experiment device |
| CN204550528U (en) * | 2015-03-26 | 2015-08-12 | 中国人民解放军第四军医大学 | A kind of cell scuffing experimental device |
| CN205774533U (en) * | 2016-05-24 | 2016-12-07 | 黄嘉炜 | A kind of cell scratch experiment incubator |
| CN205803456U (en) * | 2016-07-14 | 2016-12-14 | 李子超 | Cell scratch experiment six orifice plates |
| CN206385125U (en) * | 2017-01-01 | 2017-08-08 | 重庆科技学院 | Cell scratch experiment cut lid |
Non-Patent Citations (1)
| Title |
|---|
| 梁光波等: "体外定量测定细胞迁移方法的建立及应用", 《中国病理生理杂志》 * |
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| CN106701534B (en) | 2019-04-05 |
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