CN206385125U - Cell scratch experiment cut lid - Google Patents

Abstract

The utility model provides a kind of cell scratch experiment cut lid, it is characterised in that：Mainly it is made up of main cover and movable clamping lid two parts.Cut lid in cuboid bridge like lid, cuboid two it is long while without lid along while, the long lid for having circular arc at two ends of cuboid along while, can be buckled on the circular arc lateral wall of Tissue Culture Dish.The lid of main cover including cut lid one end is along side and cuboid lid, and the lid of the other end of cut lid is the movable clamping lid of independent separate along side.The Flat-end of the main cover of cut lid is covered without external force state with movable clamping, and by the effect of elastic stretching device, both closely fit.The utility model beneficial effect is that the utility model technical characterstic is easy to make simple, and with low cost, cut homogeneity is strong, and comparative experimental data is more accurate, simple, convenient.

Description

Cell scratch experiment cut lid

Technical field

The utility model is related to a kind of small device for cell experiment, from using being a kind of bioengineering and doctor Cell growth migrates the cut lid of experiment in terms of.

Background technology

There is the ability of invasion and attack and transfer to be for tumor tissue, the proliferation out of control of cancerous tissue, the disdifferentiation of oncocyte, oncocyte The most basic biological property of malignant tumour, and attack and transfer be malignant tumour threat patient health so that life it is main Reason.Therefore the rule and its mechanism of invasion and metastasis of tumor are studied, the preventing and treating to malignant tumour is significant.Cell Scarification is to determine one of method of kinetic characteristic of tumour cell.It uses for reference point of the proliferation out of control of tumor tissue, oncocyte Changing exception, knurl, cancer cell, there is the ability of invasion and attack and transfer to be that malignant tumour most basal cell's scarification is that simple and direct measure cell is moved The method of shifting movement and repair ability, similar external wound healing model, in vitro culture dish or the monolayer adherence of flat board culture On cell, with micro pipette tips or other hard things in the middle section cut of cell growth, the cell of middle body, Ran Houji are removed Continuous culture cell takes out Tissue Culture Plate to the time for testing setting, and whether observation periphery cell grows reparation to central cut Area, the growth transfer ability of cell is judged with this.Experiment need to generally set Normal group and experimental group, and experimental group is to have added certain The group of processing factor or medicine, allogenic gene etc. is planted, passes through reparation energy of the cell between different grouping for cut area Power, it can be determined that the migration of each group cell and repair ability.

However, regular growth scratch experiment is all to complete the cut of cell than ruler with pipette tips, pipette tips requirement is vertical, It can not tilt, still, in operation, due to each gimmick error, cause the vestige width marked to completely unify, contrast The effect of experiment can not be very accurate.Cell scratch experiment needs are a kind of professional, mark the consistent experimental provision of width size.

Accordingly, it would be desirable to a kind of scoring devices of professional cell growth observation, for bioengineering and medical research In cell scratch experiment.

The content of the invention

In order to solve the above problems, the technical solution of the utility model is：A kind of cell scratch experiment cut lid, mainly It is made up of main cover and movable clamping lid two parts.It is characterized in that：Cut lid is in the bridge like lid of a cuboid, rectangular Body two it is long while without lid along while, the long lid for having circular arc at two ends of cuboid along while, Tissue Culture Dish can be buckled in On circular arc lateral wall.The lid of the main cover including cut lid one end is along side and cuboid lid, the lid of the other end of cut lid Son is the movable clamping lid of independent separate along side；Be machined with the cuboid lid face of main cover 1 or 2 it is being parallel to each other, On the sliding-rail groove in insertion cuboid lid face, sliding-rail groove processing be provided with can along sliding-rail groove planar movement crosshead shoe. In the cuboid lid body of main cover, on the rectangular axis in movable clamping lid side, tensioning chamber is machined with, intracavitary is tensioned Portion is provided with elastic stretching device.Elastic stretching device is, in natural shrinking such as spring etc., external force to be added when pulling open, and removes external force Afterwards, original state is shunk back again.2 flat chambers of inserted sheet are machined with the cuboid lid body of the parallel both sides of tensioning chamber；It is tensioned chamber and 2 The individual flat chamber of inserted sheet is all machined with opening on the end face towards movable clamping lid.The movable clamping is covered in correspondence tensioning chamber and 2 On the position of the flat chamber opening of inserted sheet, processing is fixed with 1 elastic threads and inserted sheet is fixed at 2 right angles.Elastic threads are opened by being tensioned chamber Mouth and the elastic stretching device in tensioning intracavitary portion are fixedly connected.Purpose of design makes movable clamping lid closely to be pasted with main cover all the time Close.2 right angles fix inserted sheet and are inserted into or extract 2 corresponding flat chambers of inserted sheet.Without external force state, in elastic stretching device In the presence of, Flat-end and the movable clamping of the main cover of cut lid cover tightly close fit.The design of inserted sheet is fixed at right angle, is ensured Under the active force of tensioning apparatus, the end face that movable clamping lid all the time can be with main cover is at right angles brought into close contact, do not misplace, no On turn over.To ensure that cell scratch experiment lid can be brought into close contact the outer wall of Tissue Culture Dish.

In above-mentioned technical proposal, the crosshead shoe is orthogonal cross-shaped in two pieces of flat cuboids, one piece Prolate cube can be embedded in sliding-rail groove and slide, and the cuboid lid face of another piece of prolate cube vertically and main cover is smooth to be connect Touch.Design secures crosshead shoe, facilitates hand push crosshead shoe again.Up/down perforation is machined with the crossing center of crosshead shoe The pin holes of sliding block, pin holes can install cut pin, the respectively different thickness specification of cut pin tip diameter.This design side of being Just cut pin is changed.The specification of cut lid and Tissue Culture Dish standard size are supporting, in order to support the use.

The utility model technical characterstic is simple and convenient and has professional.The utility model has following beneficial effect：（1） It is easy to make simple, it is with low cost.（2）Cut homogeneity is strong, and comparative experimental data is more accurate.（3）It is simple, convenient.

Brief description of the drawings

Fig. 1 assembles the half-sectional schematic front view used to be of the present utility model.

Fig. 2 is that the utility model figure assembles the schematic top plan view used.

Fig. 3 is A-A schematic cross-sectional views of the present utility model.

Fig. 4 is crosshead shoe schematic top plan view of the present utility model.

Fig. 5 is the schematic front view that crosshead shoe of the present utility model installs scriber.

In figure：1. cut lid；2. main cover；3. crosshead shoe；4. cut pin；5. it is tensioned chamber；6. elastic stretching device；7. bullet Property rope；8. movable clamping lid；9. Tissue Culture Dish；10. cellular layer；11. the flat chamber of inserted sheet；12. inserted sheet is fixed at right angle；13. slide rail Groove；14. pin holes.

Embodiment

Further the utility model is illustrated with reference to the accompanying drawings and examples.

Embodiment one

Referring to figs. 1 to Fig. 5 shape and structure, a kind of cell scratch experiment cut lid, the specification of cut lid 1 is：Most greatly enhance Degree, has diameter=100mm of circular arc equivalent to the long side two ends of the cuboid of cut lid 1, there is 2 sliding-rail grooves being parallel to each other 13, the Tissue Culture Dish for being suitable for diameter 100mm is used.Use diameter=2mm cut pin 4.

Embodiment two

Referring to figs. 1 to Fig. 5 shape and structure, a kind of cell scratch experiment cut lid, the specification of cut lid 1 is：Most greatly enhance Degree, has diameter=60mm of circular arc equivalent to the long side two ends of the cuboid of cut lid 1, there is 2 sliding-rail grooves being parallel to each other 13, the Tissue Culture Dish for being suitable for diameter 60mm is used.Diameter=1.5mm cut pin 4.

Embodiment three

Referring to figs. 1 to Fig. 5 shape and structure, a kind of cell scratch experiment cut lid, the specification of cut lid 1 is：Most greatly enhance Degree, has diameter=35mm of circular arc equivalent to the long side two ends of the cuboid of cut lid 1, there is 1 sliding-rail groove being parallel to each other 13, the Tissue Culture Dish for being suitable for diameter 35mm is used.Diameter=1mm cut pin 4.

Claims (2)

1. a kind of cell scratch experiment cut lid, mainly by main cover（2）With movable clamping lid（8）Two parts are constituted, its feature It is：Cut lid（1）In the bridge like lid of a cuboid, cuboid two it is long while without lid along while, the length of cuboid Have at two ends the lid of circular arc along while, can be buckled on the circular arc lateral wall of Tissue Culture Dish；The main cover（2）Including cut Lid（1）The lid of one end is along side and cuboid lid, cut lid（1）The other end lid along side be independent separate active card Tight lid（8）；In main cover（2）Cuboid lid face on be machined with 1 or 2 it is being parallel to each other, insertion cuboid lid face Sliding-rail groove（13）, sliding-rail groove（13）Upper processing is provided with can be along sliding-rail groove（13）The crosshead shoe of planar movement（3）；In main cover （2）In cuboid lid body, close to movable clamping lid（8）On the rectangular axis in side, tensioning chamber is machined with（5）, tensioning Chamber（5）Inside is provided with elastic stretching device（6）；It is tensioned chamber（5）2 inserted sheets are machined with the cuboid lid body of parallel both sides Flat chamber（11）；It is tensioned chamber（5）With 2 flat chambers of inserted sheet（11）All towards movable clamping lid（8）End face on be machined with opening；It is described Movable clamping lid（8）Chamber is tensioned in correspondence（5）With 2 flat chambers of inserted sheet（11）On the position of opening, processing is fixed with 1 elastic threads （7）Inserted sheet is fixed with 2 right angles（12）；Elastic threads（7）By being tensioned chamber（5）Opening and tensioning chamber（5）Internal elastic tension Device（6）It is fixedly connected；Inserted sheet is fixed at 2 right angles（12）It is inserted into or extracts 2 corresponding flat chambers of inserted sheet（11）；Without outer Power state, in elastic stretching device（6）In the presence of, cut lid（1）Main cover（2）Flat-end and movable clamping lid（8） Closely fit.

2. a kind of cell scratch experiment cut lid according to claim 1, it is characterised in that：The crosshead shoe（3） Orthogonal cross-shaped in two pieces of flat cuboids, one piece of prolate cube can be embedded in sliding-rail groove（13）It is interior to slide, hang down Straight another piece of prolate cube and main cover（2）Cuboid lid face smooth contact；In crosshead shoe（3）Crossing center processing There are the pin holes of up/down perforation sliding block（14）, pin holes（14）Cut pin can be installed（4）, cut pin（4）Tip diameter is distinguished Different thicknesses specification；Cut lid（1）Specification and Tissue Culture Dish（9）Standard size is supporting.