CN106701534B - A kind of application method of cell scratch experiment lid - Google Patents
A kind of application method of cell scratch experiment lid Download PDFInfo
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- CN106701534B CN106701534B CN201710000016.6A CN201710000016A CN106701534B CN 106701534 B CN106701534 B CN 106701534B CN 201710000016 A CN201710000016 A CN 201710000016A CN 106701534 B CN106701534 B CN 106701534B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
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Abstract
The present invention provides a kind of application methods of cell scratch experiment lid, it is characterized by: the scratch lid used is in the bridge like lid of a cuboid, in two long side of cuboid without lid along side, the long side both ends of cuboid have arc-shaped lid along side, can be buckled on the circular arc lateral wall of Tissue Culture Dish.Main cover includes the lid of scratch lid one end along side and cuboid lid, and the lid of the other end of scratch lid is the movable clamping lid of independent separate along side.The Flat-end and movable clamping Gai Wu external force state of the main cover of scratch lid, by the effect of elastic stretching device, the two closely fits.Application method of the invention includes: installation, contact pin, scratch, takes needle.Present invention has the advantages that the technology of the present invention feature be it is simple and easy to make, low in cost, scratch identity is strong, and comparative experimental data is more accurate, simple, convenient.
Description
Technical field
The present invention relates to a kind of application methods of small device for cell experiment, further particularly say, are a kind of lifes
The application method of the cell scratch lid of cell growth migration experiment in terms of object engineering and medicine.
Technical background
There is the ability of invasion and transfer to be for tumor tissue, the proliferation out of control of cancerous tissue, the disdifferentiation of oncocyte, oncocyte
The most basic biological property of malignant tumour, and invade and transfer be malignant tumour threat patient health so that life it is main
Reason.Therefore the rule and its mechanism of invasion and metastasis of tumor are studied, it is significant to the prevention and treatment of malignant tumour.Cell
Scarification is to determine one of method of kinetic characteristic of tumour cell.Point of its proliferation out of control for using for reference tumor tissue, oncocyte
It is that simple and direct measurement cell moves that it is malignant tumour most basal cell's scarification that change exception, tumor, cancer cell, which have the ability of invasion and transfer,
The method of shifting movement and repair ability, similar external wound healing model, in vitro culture dish or the monolayer adherence of plate culture
On cell, the middle section scratch grown with micro pipette tips or other hard objects in cell removes the cell of center portion, then after
Continuous culture cell takes out tissue culture plate, whether observation periphery cell grows reparation to central scratch to the time of experiment setting
Area judges the growth transfer ability of cell with this.Experiment need to usually set Normal group and experimental group, and experimental group is to have added certain
The group for planting processing factor or drug, allogenic gene etc., by the cell between different grouping for the reparation energy in scratch area
Power, it can be determined that the migration of group of cells and repair ability.
However, regular growth scratch experiment is all the scratch for completing cell than ruler with pipette tips, pipette tips are required vertically,
It cannot tilt, still, in operation, due to each gimmick error, cause the trace width marked that cannot completely unify, compare
The effect of experiment cannot be very accurate.Cell scratch experiment need it is a kind of profession, mark the consistent experimental provision of width size.
Therefore, it is necessary to the scoring devices and application method of a kind of growth observation of the cell of profession, for bioengineering and
In the cell scratch experiment of medical research.
Summary of the invention
To solve the above-mentioned problems, the technical solution of the present invention is as follows: a kind of application method of cell scratch experiment lid, uses
Cell scratch experiment scratch lid, be mainly made of main cover and movable clamping lid two parts.It is characterized by: scratch Gai Chengyi
The bridge like lid of a cuboid, in two long side of cuboid without lid along side, there is arc-shaped lid at the long side both ends of cuboid
Son can be buckled on the circular arc lateral wall of Tissue Culture Dish along side.The main cover includes the lid of scratch lid one end along side and length
Cube lid, the lid of the other end of scratch lid are the movable clamping lid of independent separate along side；In the cuboid lid face of main cover
On be machined with 1 or 2 sliding-rail groove be parallel to each other, perforation cuboid lid face, be equipped with being capable of edge for processing on sliding-rail groove
The crosshead shoe of sliding-rail groove planar movement.In the cuboid lid body of main cover, close to movable clamping lid side it is rectangular in
On axis, it is machined with tensioning chamber, intracavitary portion is tensioned and elastic stretching device is installed.Elastic stretching device is in natural shrinking such as bullet
Spring etc. pulls open Shi Yaojia external force, after removing external force, and shrinks back original state.It is tensioned the cuboid lid body of the parallel two sides of chamber
Inside it is machined with 2 flat chambers of inserted sheet；Tensioning chamber and 2 flat chambers of inserted sheet are all machined with opening on the end face towards movable clamping lid.Institute
It states movable clamping to cover on the position of corresponding tensioning chamber and the flat chamber opening of 2 inserted sheets, processing is fixed with 1 elastic string and 2 straight
Inserted sheet is fixed at angle.Elastic string is fixedly connected by tensioning chamber opening and the elastic stretching device in the intracavitary portion of tensioning.Purpose of design makes
Movable clamping lid can fit closely always with main cover.It is flat that the fixed inserted sheet in 2 right angles is inserted into or extracts 2 corresponding inserted sheets
Chamber.Without external force state, under the action of elastic stretching device, Flat-end and the movable clamping of the main cover of scratch lid cover tightly close phase
Fitting.The design of the fixed inserted sheet in right angle, has ensured under the active force of tensioning apparatus, movable clamping lid always can be with the end of main cover
Face at right angles fits closely, and does not misplace, and does not turn over.To guarantee that cell scratch experiment lid can fit closely Tissue Culture Dish
Outer wall.The application method of a kind of cell scratch lid of above-mentioned technical proposal, which is characterized in that operating procedure is as follows.
The first step, installation.The scratch lid with the Tissue Culture Dish units specification of experiment is chosen, Tissue Culture Dish is removed
Lid, unplugs movable clamping lid, by scratch cover buckle in Tissue Culture Dish.
Second step, contact pin.After fixed clamping scratch lid, crosshead shoe is moved on to the top of sliding-rail groove one end, chooses experiment
It is required that diameter scratch needle, be inserted into the pin holes of crosshead shoe, adjustment height, until scratch needle pass through Tissue Culture Dish
The cellular layer of bottom is until Tissue Culture Dish bottom, clamping fixed scratch needle.
Third step, scratch.It pins crosshead shoe and at the uniform velocity slides into other end top from the top of sliding-rail groove, complete cell and draw
Trace；If main cover is there are two sliding-rail groove, the operation of second sliding-rail groove repeats second step and third step.
4th step takes out scratch needle, unplugs movable clamping lid, remove and remove scratch lid, cover the lid of Tissue Culture Dish.
A kind of application method of cell scratch lid described in above-mentioned technical proposal, which is characterized in that the crosshead shoe used
Orthogonal cross-shaped in two pieces of flat cuboids, one piece of prolate cube can be embedded in sliding in sliding-rail groove, vertically
The cuboid lid face smooth contact of another piece of prolate cube and main cover.Design secures crosshead shoe, and facilitates hand push ten
Word sliding block.The pin holes of sliding block up and down are machined in the crossing center of crosshead shoe, pin holes can install scratch needle, draw
The respectively different thickness specification of trace needle tip diameter.This design is to be convenient for changing scratch needle.The specification and cell of scratch lid
Culture dish standard size is mating, in order to match.
The technology of the present invention feature be it is simple and convenient, have again it is professional.The present invention has following the utility model has the advantages that (1) is easy to make
Simply, low in cost.(2) scratch identity is strong, and comparative experimental data is more accurate.(3) simple, convenient.
Detailed description of the invention
Fig. 1 is the half-sectional schematic front view that assembly of the invention uses.
Fig. 2 is the schematic top plan view that present invention figure assembly uses.
Fig. 3 is A-A schematic cross-sectional view of the invention.
Fig. 4 is crosshead shoe schematic top plan view of the invention.
Fig. 5 is the schematic front view that crosshead shoe of the invention installs scriber.
In figure: 1. scratch lids；2. main cover；3. crosshead shoe；4. scratch needle；5. being tensioned chamber；6. elastic stretching device；7. bullet
Property rope；8. movable clamping lid；9. Tissue Culture Dish；10. cellular layer；11. the flat chamber of inserted sheet；12. inserted sheet is fixed at right angle；13. sliding rail
Slot；14. pin holes.
Specific embodiment
Further the present invention is illustrated with reference to the accompanying drawings and examples.
Embodiment one
Referring to figs. 1 to the shape and structure of Fig. 5, a kind of cell scratch experiment lid and application method, 1 specification of scratch lid are as follows: most
Long length, equal to the arc-shaped diameter of the long side both ends processing of 1 cuboid of scratch lid, diameter=100mm；Scratch lid 1 has 2
The sliding-rail groove 13 that item is parallel to each other.The Tissue Culture Dish for being suitable for diameter 100mm uses.It is as follows using operating procedure.
The first step removes the lid of the Tissue Culture Dish 9 of diameter 100mm, unplugs movable clamping lid 8, scratch lid 1 is buckled in
Tissue Culture Dish 9.
Crosshead shoe 3 after fixing clamping scratch lid 1, is moved on to the top of 13 one end of sliding-rail groove, chooses diameter by second step
The scratch needle 4 of=2mm is inserted into the pin holes 14 of crosshead shoe 3, adjustment height, until scratch needle 4 passes through 9 bottom of Tissue Culture Dish
Cellular layer 10 until 9 bottom of Tissue Culture Dish.Clamping fixed scratch needle 4.
Third step pins crosshead shoe 3, the prolate cube vertical with sliding-rail groove 13 of crosshead shoe 3 is at the uniform velocity pushed away, from sliding-rail groove
13 top slides into other end top, completes cell scratch.Then, scratch needle 4 is extracted, ten be inserted on Article 2 sliding-rail groove 13
In the pin holes 14 of word sliding block 3, second step and third step operation are repeated.
4th step takes out scratch needle 4, unplugs movable clamping lid 8, remove and remove scratch lid 1, cover Tissue Culture Dish 9
Lid.
Embodiment two
Referring to figs. 1 to the shape and structure of Fig. 5, a kind of cell scratch experiment lid and application method, 1 specification of scratch lid are as follows: most
Long length, equal to the arc-shaped diameter of the long side both ends processing of 1 cuboid of scratch lid, diameter=60mm；Scratch lid 1 has 2
The sliding-rail groove 13 being parallel to each other.Use diameter=1.5mm scratch needle 4.The Tissue Culture Dish for being suitable for diameter 60mm makes
With.Operation is referring to embodiment one.
Embodiment three
Referring to figs. 1 to the shape and structure of Fig. 5, a kind of cell scratch experiment lid and application method, 1 specification of scratch lid are as follows: most
Long length, equal to the arc-shaped diameter of the long side both ends processing of 1 cuboid of scratch lid, diameter=35mm；Scratch lid 1 has 1
The sliding-rail groove 13 being parallel to each other.The Tissue Culture Dish for being suitable for diameter 35mm uses.It is as follows using operating procedure.
The first step removes the lid of the Tissue Culture Dish 9 of diameter 35mm, unplugs movable clamping lid 8, scratch lid 1 is buckled in
Tissue Culture Dish 9.
Crosshead shoe 3 after fixing clamping scratch lid 1, is moved on to the top of 13 one end of sliding-rail groove, chooses diameter by second step
The scratch needle 4 of=1mm is inserted into the pin holes 14 of crosshead shoe 3, adjustment height, until scratch needle 4 passes through 9 bottom of Tissue Culture Dish
Cellular layer 10 until 9 bottom of Tissue Culture Dish.Clamping fixed scratch needle 4.
Third step pins crosshead shoe 3, the prolate cube vertical with sliding-rail groove 13 of crosshead shoe 3 is at the uniform velocity pushed away, from sliding-rail groove
13 top slides into other end top, completes cell scratch.
4th step takes out scratch needle 4, unplugs movable clamping lid 8, remove and remove scratch lid 1, cover Tissue Culture Dish 9
Lid.
Claims (2)
1. a kind of application method of cell scratch experiment lid, the scratch lid (1) of the cell scratch experiment used is mainly by main cover (2)
It is formed with movable clamping lid (8) two parts；The main cover (2) includes the lid being arc-shaped set on described scratch lid (1) one end
The other end of the lid that son is rectangle along side and cross section, the scratch lid (1) is the movable clamping lid (8) of independent separate；
Edge of the front and rear sides face without lid of the lid of scratch lid (1) main cover (2) being rectangle, the lid that cross section is rectangle
Sub- both ends processing is arc-shaped, and wherein one end is fitted closely with the arc-shaped lid edge, and the arc-shaped lid
Edge can be buckled on the circular arc lateral wall of Tissue Culture Dish；And the other end of the cap body and arc-shaped movable clamping
Lid (8) closely fits, and movable clamping lid (8) can be buckled on the circular arc lateral wall of Tissue Culture Dish；It is located at described draw
The lid edge and movable clamping lid at trace lid (1) both ends make it in a bridge like lid；It is rectangle in the cross section of main cover (2)
1 or 2 sliding-rail groove (13) be parallel to each other, the perforation lid face, sliding-rail groove (13) are machined on the lid face of lid
Upper processing is equipped with can be along the crosshead shoe (3) of sliding-rail groove (13) planar movement；In the lid body of main cover (2), close to living
On dynamic clamping lid (8) rectangular central axes in side, it is machined with tensioning chamber (5), elastic tension dress is installed inside tensioning chamber (5)
Set (6)；2 flat chambers of inserted sheet (11) are machined in the lid body of the parallel two sides of tensioning chamber (5)；It is tensioned chamber (5) and 2 flat chambers of inserted sheet
(11) opening is all machined on the end face towards movable clamping lid (8)；The movable clamping lid (8) is tensioned chamber (5) and 2 corresponding
On the position of a flat chamber of inserted sheet (11) opening, processing is fixed with 1 elastic string (7) and 2 right angles are fixed inserted sheet (12)；Elastic string
(7) fixedly connected by tensioning chamber (5) the opening elastic stretching device (6) internal with tensioning chamber (5)；The fixed inserted sheet in 2 right angles
(12) it is inserted into or extracts 2 corresponding flat chambers of inserted sheet (11)；Effect without external force state, in elastic stretching device (6)
Under, the other end and the movable clamping lid (8) of the main cover (2) of scratch lid (1) closely fit；It is characterized in that, operating procedure is such as
Under:
The first step, installation choose the scratch lid (1) with Tissue Culture Dish (9) units specification of experiment, remove Tissue Culture Dish
(9) it covers, unplugs movable clamping lid (8), scratch lid (1) is buckled in Tissue Culture Dish (9)；
Crosshead shoe (3) is moved on to the top of sliding-rail groove (13) one end after fixed clamping scratch lid (1), selected by second step, contact pin
The scratch needle (4) of the diameter of requirement of experiment is taken, is inserted into the pin holes (14) of crosshead shoe (3), adjustment height, until scratch
Needle (4) passes through the cellular layer (10) of Tissue Culture Dish (9) bottom until Tissue Culture Dish (9) bottom, clamping fixed scratch needle
(4)；
Third step, scratch pin crosshead shoe (3) and at the uniform velocity slide into other end top from the top of sliding-rail groove (13), complete cell
Scratch；If any second sliding-rail groove (13), operation repeats second step and third step；
4th step is taken out scratch needle (4), unplugs movable clamping lid (8), remove and remove scratch lid (1), cover Tissue Culture Dish
(9) lid.
2. a kind of application method of cell scratch experiment lid according to claim 1, it is characterised in that: the cross used is sliding
Block (3) is orthogonal cross-shaped in two pieces of flat cuboids, and one piece of prolate cube can be embedded in sliding-rail groove (13) sliding
It is dynamic, the lid face smooth contact of the lid of vertical another piece of prolate cube and main cover (2)；In the intersection of crosshead shoe (3)
The heart is machined with the pin holes (14) of sliding block up and down, and pin holes (14) can be installed scratch needle (4), and scratch needle (4) is according to it
Tip diameter is divided into the specification of different thicknesses；The specification of scratch lid (1) and Tissue Culture Dish (9) standard size are mating.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710000016.6A CN106701534B (en) | 2017-01-01 | 2017-01-01 | A kind of application method of cell scratch experiment lid |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710000016.6A CN106701534B (en) | 2017-01-01 | 2017-01-01 | A kind of application method of cell scratch experiment lid |
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| CN106701534A CN106701534A (en) | 2017-05-24 |
| CN106701534B true CN106701534B (en) | 2019-04-05 |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1596344A (en) * | 2001-11-27 | 2005-03-16 | 赫伯特·汉切恩两合公司 | Clamping device and regulating unit |
| CN203728831U (en) * | 2014-03-19 | 2014-07-23 | 中南大学湘雅医院 | Cell scratch experiment device |
| CN204550528U (en) * | 2015-03-26 | 2015-08-12 | 中国人民解放军第四军医大学 | A kind of cell scuffing experimental device |
| CN205774533U (en) * | 2016-05-24 | 2016-12-07 | 黄嘉炜 | A kind of cell scratch experiment incubator |
| CN205803456U (en) * | 2016-07-14 | 2016-12-14 | 李子超 | Cell scratch experiment six orifice plates |
| CN206385125U (en) * | 2017-01-01 | 2017-08-08 | 重庆科技学院 | Cell scratch experiment cut lid |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110195489A1 (en) * | 2010-02-08 | 2011-08-11 | Rongpei Lan | System and Method of an In-vitro Wound Healing Model on Cultured Cell Monolayer Employing Stamp Device |
-
2017
- 2017-01-01 CN CN201710000016.6A patent/CN106701534B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1596344A (en) * | 2001-11-27 | 2005-03-16 | 赫伯特·汉切恩两合公司 | Clamping device and regulating unit |
| CN203728831U (en) * | 2014-03-19 | 2014-07-23 | 中南大学湘雅医院 | Cell scratch experiment device |
| CN204550528U (en) * | 2015-03-26 | 2015-08-12 | 中国人民解放军第四军医大学 | A kind of cell scuffing experimental device |
| CN205774533U (en) * | 2016-05-24 | 2016-12-07 | 黄嘉炜 | A kind of cell scratch experiment incubator |
| CN205803456U (en) * | 2016-07-14 | 2016-12-14 | 李子超 | Cell scratch experiment six orifice plates |
| CN206385125U (en) * | 2017-01-01 | 2017-08-08 | 重庆科技学院 | Cell scratch experiment cut lid |
Non-Patent Citations (1)
| Title |
|---|
| 体外定量测定细胞迁移方法的建立及应用;梁光波等;《中国病理生理杂志》;20041231;第20卷(第2期);第175-179页 |
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| CN106701534A (en) | 2017-05-24 |
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