CN203112818U

CN203112818U - Tip box

Info

Publication number: CN203112818U
Application number: CN 201320108912
Authority: CN
Inventors: 徐加英; 秦立强; 焦旸; 柴之芳; 樊赛军
Original assignee: Suzhou University
Current assignee: Suzhou University
Priority date: 2013-03-11
Filing date: 2013-03-11
Publication date: 2013-08-07
Anticipated expiration: 2023-03-11

Abstract

The utility model discloses a tip box. The tip box comprises a box cover, a box bottom and an insert plate arranged at the upper part of the box bottom, is characterized in that run-through holes with at least two different apertures are distributed in the insert plate. The tip box provided by the utility model can simultaneously place tips with various specifications, can greatly save experimental operation time, enables the operation to be more convenient, is flexible in use, can save material consumption in experiments, and is simple in structure, easy to process and manufacture, and suitable for promotion and use at the same time.

Description

A kind of rifle head box

Technical field

The utility model relates to the device that field of cell culture is used, and is specifically related to a kind of be used to the rifle head box that leaves the rifle head that Transwell method experiment middle berth glue uses in.

Background technology

According to the supposition of IARC (IARC) to nineteen ninety and global tumor invasion death in 2000, nineteen ninety whole cancer new cases 8,100,000, death 5,200,000; New cases 1,010 ten thousand in 2000, and dead 6,200,000.Compare two periods, and pathogenesis of cancer and dead routine number are obvious ascendant trend, and new cases have increased by 24.7%, and death has increased by 19.2%.WHO world DKFZ global monitoring data presentation: global cancer new cases 1,300 ten thousand in 2008, dead 7,600,000, in per 8 deaths 1 routine cancer patients is just arranged.WHO warns, as not adopting an effective measure, trend development like this, to the year two thousand thirty the whole world 2,600 ten thousand newly-increased cases of cancers will be arranged, dead routine number will jump to 1,700 ten thousand.Malignant tumour is one of important diseases of current harm humans health, is human first killer of new millennium, and human survival is constituted the most serious threat.

Matrigel matrigel/basilar membrane is to extract basement membrane matrix from the EHS mouse tumor that is rich in extracellular matrix protein, is applicable to the three-dimensional cell cultivation.At ambient temperature, the Matrigel polymerization forms the three dimensional matrix with biologic activity, structure, composition, physical property and the function of analogue body inner cell basilar membrane, be conducive to cultivation and the differentiation of cell in vitro, can be used for cellular form, biochemical function, move, infect, multinomial research such as genetic expression.Because polymerization takes place under normal temperature condition Matrigel soon, therefore in the test of using Matrigel, must use transfer pipet, the rifle of precooling first-class.As in order to observe the invasion by tumor cells ability; usually can adopt the Transwell method; in these trials in order to guarantee that rapid solidification does not take place Matrigel glue in operating process; reach desirable experiment effect, need be to the rifle head of test middle berth glue and Transwell cell and 24 orifice plates etc. in the refrigerator precooling.

Yet, the rifle head box that buys on the market, a kind of specification rifle head box has only the rifle head of a specification, shown in accompanying drawing 1.As, Chinese invention patent application CN102921484A discloses a kind of liquid-transfering gun rifle head box-shaped structure, it comprises at the bottom of lid, the box, upper cap at the bottom of the box is equipped with support, the installation all around that the edge of described support is installed at the bottom of the described box is protruded, be evenly equipped with through hole on the described support, the liquid-transfering gun head is plugged in described through hole, leaves spacing between the bottom at the bottom of the bottom surface of described support and the described box.In this scheme, through hole is distributed on the support, and from accompanying drawing as can be seen, the size of through hole is consistent.In the commercially available prod, common have 1ml rifle head box, 200 μ l rifle head boxes and 10 μ l rifle head boxes, is used for depositing a kind of rifle head of regulation in every kind of rifle head box.

Usually need to use the rifle head of different size in the actual experiment process, this carries out precooling treatment with regard to the rifle head box that needs to prepare plurality of specifications, thus, has taken more experimental implementation space, and picks and places rifle head operation inconvenience.

Summary of the invention

Goal of the invention of the present utility model provides a kind of rifle head box, can place the rifle head of multiple different size simultaneously.

To achieve the above object of the invention, the technical solution adopted in the utility model is: a kind of rifle head box, comprise at the bottom of lid, the box and be located at box at the bottom of the plate on top, be distributed with the through hole at least 2 kinds of different apertures on the described plate.

Be removable the connection between at the bottom of the further technical scheme, described plate and box, described plate is provided with a handle that raises up.Change the different size plate with convenient according to the different experiments demand.

Preferably, described plate is distributed with the through hole in 3 kinds of different apertures, and 3 kinds of through holes cooperate with 1ml rifle head, 200 μ l rifle heads and 10 μ l rifle heads respectively.

In the technique scheme, the rear portion is connected through hinge at the bottom of described lid and the box, and the front is fastened through snap close.So that maintenance sterile state.

Further technical scheme, the through hole place in described different apertures is provided with the mark of different colours.Better identification is to reduce the probability of wrong plug rifle head in the experimentation.

In the technique scheme, described rifle head box length is 10～12.5cm, and width is 8～10cm, highly is 8～10cm.

Because technique scheme is used, the utility model compared with prior art has following advantage:

1. the rifle head box that provides of the utility model can be placed the rifle head of plurality of specifications simultaneously, saves the experimental implementation space greatly, makes more convenient to operate.

2. plate of the present utility model is replaceable, selects for use the different size jack to cooperate according to the experiment demand, uses flexibly, thereby once can only use a rifle head box in the experiment, saves the experiment consumptive material.

3. the utility model can arrange the mark of different colours according to the aperture difference on through hole, avoids misoperation.

4. the utility model is simple in structure, is easy to processing and fabricating, is suitable for promoting the use.

Description of drawings

Fig. 1 is the plate in the rifle head box or support schematic top plan view in the prior art.

Fig. 2 is the structural representation of the utility model embodiment one.

Fig. 3 is the synoptic diagram of the plate among Fig. 2.

Wherein: 1, lid; 2, at the bottom of the box; 3, snap close; 4, plate; 5, through hole; 6, handle.

Embodiment

Below in conjunction with drawings and Examples the utility model is further described:

Embodiment one: referring to shown in Figure 2, a kind of rifle head box comprises at the bottom of lid 1, the box 2 and be located at the plate 4 on 2 tops at the bottom of the box, and 2 rear portions are connected through hinge at the bottom of described lid 1 and the box, and the front is fastened through snap close 3.2 are removable the connection at the bottom of described plate 4 and the box, and described plate 4 is provided with a handle that raises up 6.Be distributed with the through hole 5 in 3 kinds of different apertures on the described plate 4, wherein 1 row's through hole is used for placement 1ml rifle head, 2 row's through holes are used for placing 200 μ l rifle heads, 3 row's through holes for placing 10 μ l rifle heads.

The through hole place in different apertures is provided with the mark of different colours, and for example, the through hole that is used for placing 1ml rifle head is designated as redness, and the through hole that is used for placing 200 μ l rifle heads is designated as orange, and the through hole that is used for placing 10 μ l rifle heads is designated as blueness.

The rifle head box length of present embodiment is 10～12.5cm, and width is 8～10cm, highly is 8～10cm.

Claims

1. rifle head box, the plate (4) on (2) top is characterized in that: the through hole (5) that is distributed with 2 kinds of different apertures on the described plate (4) at least at the bottom of comprising (2) at the bottom of lid (1), the box and being located at box.

2. rifle head box according to claim 1 is characterized in that: be removable the connection between (2) at the bottom of described plate (4) and the box, described plate (4) is provided with a handle that raises up (6).

3. rifle head box according to claim 1, it is characterized in that: described plate is distributed with the through hole in 3 kinds of different apertures, and 3 kinds of through holes cooperate with 1ml rifle head, 200 μ l rifle heads and 10 μ l rifle heads respectively.

4. rifle head box according to claim 1 is characterized in that: (2) rear portion is connected through hinge at the bottom of described lid (1) and the box, and the front is fastened through snap close (3).

5. rifle head box according to claim 1, it is characterized in that: the through hole place in described different apertures is provided with the mark of different colours.

6. rifle head box according to claim 1, it is characterized in that: described rifle head box length is 10～12.5cm, and width is 8～10cm, highly is 8～10cm.