CN2462387Y

CN2462387Y - Density gradient medicine sensitive test strip

Info

Publication number: CN2462387Y
Application number: CN 01217261
Authority: CN
Inventors: 张同军; 曾嵘斌
Original assignee: Individual
Current assignee: Individual
Priority date: 2001-01-21
Filing date: 2001-01-21
Publication date: 2001-11-28
Anticipated expiration: 2011-01-21

Abstract

The utility model relates to a concentration gradient medicine sensitive test strip, which is characterized in that a carrier is coated with antibiotic according to continuous concentration gradient and simultaneously marked with the scales of MICvalues. Namely the concentration of the antibiotic on any transverse straight line is the same, and the antibiotic is continuously distributed on any longitudinal straight line with indexes. The utility model integrated with the advantages of two kinds of the prior art overcomes the disadvantages in the prior art and provides a new medicine sensitive test strip for the MIC values of the antibiotic. The utility model can be used for taking a medicine sensitive test conveniently, and simultaneously, the MIC values can be tested by one step. Precision is within one two-fold dilution step, and the utility model is improved greatly as compared with the prior art.

Description

The concentration gradient test strip

The utility model relates to the concentration gradient test strip that a kind of drug sensitive experiment is used.

At medical field and pharmaceutical field, various malignant bacterias are not only needed to determine to suppress its effective microbiotic kind, must determine that also various microbiotic suppress the required least concentration of this kind bacterium (MIC), Here it is drug sensitive experiment.Drug sensitive experiment mainly adopts disk diffusion method and microbiotic dilution method in the prior art.The former places to scribble the antibiotic scraps of paper on the nutrient culture media of having inoculated bacterium, after the hatching of certain hour, when occurring antibacterial ring on the nutrient culture media, illustrates that this microbiotic has produced fungistatic effect.This method is very easy, but can only know its bacteriostasis qualitatively according to the size of antibacterial ring, can not measure the antibiotic MIC value of this kind quantitatively.Dilution method is to measure two-fold dilution's antibiotic bacteriostasis property respectively, to determine its minimum inhibitory concentration MIC.The accuracy of this method is in 3 two-fold dilution's step scopes, but needs are done a large amount of experiments, and is more loaded down with trivial details.

The application will be in conjunction with the advantage of two kinds of prior aries, and overcomes its deficiency, and a kind of brand-new drug sensitive experiment product is provided, use this new product to make drug sensitive experiment, method is very easy, can once determine antibiotic MIC value again simultaneously, and its accuracy should have significantly raising than prior art.

The technical scheme that realizes the foregoing invention purpose is: a kind of concentration gradient test strip: be coated with microbiotic according to continuous concentration gradient on carrier, indicate the scale of its MIC value simultaneously.This concentration gradient test strip is placed on inoculated on the culture of strains base, antibacterial ring occurs after hatching, antibacterial ring and strip intersection i.e. this microbiotic MIC value.Sense data very intuitively from the scale of this strip.Here said carrier adopts inert material to make, and generally can adopt plastics.Its shape for well, but also can adopt other forms with strip.Said " being coated with microbiotic according to continuous concentration gradient " is meant: antibiotic concentration is identical on horizontal arbitrary straight line; On vertical arbitrary straight line, microbiotic is the chain index distribution.Prioritization scheme is the scale with the MIC value, is printed on the antibiotic one side of strip uncoated.Can also mark antibiotic code at an end of strip.

The utility model combines the advantage of two kinds of experimental techniques in the prior art, and overcomes its shortcoming, the concentration gradient test strip that brand-new drug sensitive experiment method is provided and has realized this method.It is very easy to use this new product to make drug sensitive experiment, can once determine antibiotic MIC value again simultaneously, and its accuracy is improved largely than prior art in 1 two-fold dilution's step scope.

Now be described further with accompanying drawing in conjunction with the embodiments.

Fig. 1 is embodiment 1 a concentration gradient test strip structural representation;

Fig. 2, Fig. 3 are respectively embodiment 1 concentration gradient test strip positive and negative view;

Fig. 4 is antibacterial ring and reading synoptic diagram.

Embodiment 1, with reference to Fig. 1, Fig. 2, Fig. 3: the concentration gradient test strip, be coated with microbiotic 2 in the one side of strip plastic carrier bar l according to continuous concentration gradient, antibiotic concentration is identical on horizontal arbitrary straight line; On vertical arbitrary straight line, microbiotic is the chain index distribution.Simultaneously, indicating with ug/ml at the another side of strip is the MIC interpretation scale 3 of unit.The antibiotic code 4 of an end (can be described as pommel) mark at strip.The preparation and the inoculation method of nutrient culture media and inoculum are same as the prior art, guarantee vaccinated agar medium dry tack free before the application of sample, clamp the pommel of strip with tweezers, (promptly have the one side of MIC value to make progress) downwards the test strip powder and be placed on the nutrient culture media, the maximum end of concentration is near the edge of double dish.According to the size of double dish, can place one or more than one strips.Gently press strip with tweezers in case of necessity, discharge the bubble under the strip, and should upwards drive bubble from an end of concentration minimum.Not removable strip after putting well.After putting well, microbiotic is diffused into immediately and forms antibiotic concentration continually varying index gradient on the agar.With reference to Fig. 4: reach the growth of time of hatching and bacterium when high-visible, can read this antibiotic MIC value at the intersection 6 of antibacterial elliptical ring 5 and test strip.When bacterium grows along whole strip, promptly there is not antibacterial ring when visible, the MIC value is greater than the maximal value of strip scale; Below antibacterial elliptical ring extended strip, when promptly not having focus with strip, the MIC value was less than the minimum value of strip scale.

Claims

1, a kind of concentration gradient test strip, it is characterized in that: be coated with microbiotic according to continuous concentration gradient on carrier, antibiotic concentration is identical on horizontal arbitrary straight line, on vertical arbitrary straight line, microbiotic is the chain index distribution, indicates the scale of its MIC value simultaneously.

2, according to the described concentration gradient test strip of claim 1, it is characterized in that: the scale of said MIC value is in the antibiotic one side of strip uncoated.

3, according to claim 1 or 2 described concentration gradient test strips, it is characterized in that: the end at strip has also marked antibiotic code.