CN100414298C - Stepped concentration gradient detecting bar and preparing process thereof - Google Patents
Stepped concentration gradient detecting bar and preparing process thereof Download PDFInfo
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- CN100414298C CN100414298C CNB2004100155704A CN200410015570A CN100414298C CN 100414298 C CN100414298 C CN 100414298C CN B2004100155704 A CNB2004100155704 A CN B2004100155704A CN 200410015570 A CN200410015570 A CN 200410015570A CN 100414298 C CN100414298 C CN 100414298C
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Abstract
The present invention relates to a staircase type concentration gradient detection bar which comprises a rectangular sheet-like carrier and a medicine coated on one surface of the carrier, wherein one surface of the carrier is divided into a plurality of sections with the equal area in an equal length mode, and medicine blocks in set shapes with set areas are coated on each section with the equal area in a central symmetry mode; doses of the medicine blocks present staircase type change of one section or a plurality of sections, namely that concentrations of the medicine blocks are different in different sections. However, the coating thicknesses and the coating concentrations of the medicine blocks arranged in the same section are the same, and the areas of the medicine blocks steadily increase or steadily decrease in sequence in a staircase mode. The other surface of the carrier is marked with the medicine varieties, and numerical values of the corresponding medicine concentrations are marked on the corresponding sections with the equal area. The staircase type concentration gradient medicine-sensitive detection bar of the present invention has the advantages of easy standardization, easy mass production, stable product quality, convenient operation and precise measurement.
Description
Technical field
The present invention relates to biomedical articles, more particularly, the present invention relates to a kind of responsive staged drug concentration gradient detector bar that detects of microbial medicine and preparation method thereof that is used for.
Background technology
Along with progress of modern technology, the evaluation of microorganism can be satisfied people (especially clinical medical personnel's) needs substantially.But in microorganism to aspect the drug sensitivity test (abbreviation drug sensitive test), such as in drug sensitive test to severe bacteria, anaerobion, mycobacterium, fungi, aspect the conventional drug sensitive test of adopting bacterium robotization assessing instrument to be done, still exist at present some urgent problems as: under some situation the report false responsive result, for example: in the test to the intestinal of the staphylococcus aureus (MRSA) of anti-Oxacillin, enterococcus, product induced enzyme and pseudomonas aeruginosa, indivedual persisters can not show the resistance result.Particularly in recent years, because the abuse of various antibiotic extensive application, especially wide spectrums and super broad-spectrum antibiotic has caused a large amount of appearance of medicament-resistant mutation bacterial strain, experience medication mortality is higher; Blindly heavy dose of treatment also easily causes drug poisoning.So, in recent years, the important content that microbiological research relates to carries out external drug sensitivity test (promptly so-called usually " drug sensitivity test " to pathogen exactly, be called for short " drug sensitive test "), promptly measure the sensitivity of bacterium to medicine, so that in time find antibody-resistant bacterium, for clinical application provides selection scheme.The correctness of drug sensitive test directly has influence on the clinician and selects antibacterials rationally and effectively for use, is related to that the clinician correctly selects microbiotic for use, avoids abuse of antibiotics, a large amount of appearance of control infection, prevention medicament-resistant mutation bacterial strain timely and effectively.Thereby the external drug sensitivity test of pathogen has the important clinical meaning.
At present, external drug sensitivity test main method has: the agar diffusion method of the paper of qualitative determination (diskagar diffusion, Kirby-Bauer test), the dilution method (dilution test) of quantitative measurement and concentration gradient test paper method (Episiolometer test, E test).
Wherein, the agar diffusion method of the paper using method is very easy, but can only do qualitative detection, can not be quantitative, and to the requirement for experiment condition harshness, and error appears easily.Particularly in recent years, the standardization council of U.S. clinical labororatory (N C C L S) no longer recommends to measure the antibacterial action of some microbiotic to some bacterium with disk diffusion method, and the result that its reason is a disk diffusion method can cause too much misconstruction.
Dilution method is after medicine being carried out a series of (to doubly) dilution in meat soup or agar medium, quantitatively to inoculate bacterium to be checked, hatches after 24 hours for 35 ℃ and observes.The lowest concentration of drug that suppresses bacterial context eye visible growth to be checked is the minimum inhibitory concentration (MIC) of this medicine to bacterium to be checked.Especially the agar dilution measurement result is accurate for dilution method, and being healed is " goldstandard ", but operation is too loaded down with trivial details, is difficult to clinical extensive, the universal use.
AB Biodisk (Sweden) company had released the concentration gradient test paper method in 1988, had been known as the susceptibility method of " second goldstandard " by world colleague.Its feature is the advantage in conjunction with agar diffusion method and dilution method, and with the diffusion ratio juris, the form of plastic strip is quantitatively read the MIC value.This size of surveying inhibition zone than traditional disk diffusion method is accurately reliable.The concentration gradient test paper method is now admitted by U.S. food and FAD (FDA) and World Health Organization's bacterial resistance monitoring net.
Concentration gradient test paper method ultimate principle is in conjunction with diffusion method and dilution ratio juris and characteristics, simultaneously being adsorbed with log2 at the specification plastic strip that is 5 * 50mm is basic continually varying antibiotic concentration, and the corresponding antibiotic concentration scale of mark is gone up on its another surface.After being placed on the particular drug sensitive culture medium of having inoculated certain density bacterium when the concentration gradient test strips, microbiotic is released in the agar immediately, is the single direction diffusion.Under 35 ℃, after hatching, certain hour may produce an oval-shaped antibacterial ring.The scale of antibacterial ring and Etest bar intersection is minimal inhibitory concentration (MIC) value at this bacterial strain.The concentration gradient test paper method is easy to operate as diffusion method, but can be with the minimum inhibitory concentration (MIC) of the equally direct mensuration medicine of dilution method to bacterium to be checked.
At present, in worldwide, has only Sweden AB BIODISK company production concentration gradient test strips.They are that the microbiotic with a plurality of (generally at 15～22) variable concentrations adheres to preparation concentration gradient test strips on the plastic strip.Its process for producing complexity, loaded down with trivial details.
Domestic applications are arranged number has been 93245821.1 patent disclosure a kind of " the responsive gradient strip of antibacterials ", patent documentation from its document and application thereof, though its conclusion is " the responsive gradient strip of homemade antibacterials is believable ", but, because its method for making is with " 15 little scraps of paper of pastille that contain variable concentrations; press the concentration order, be attached on 5 * 50mm polyester flake with pressure-sensitive adhesive ".The defective of this method for making has: (1) process of manufacturing is difficult to standardization, can't guarantee the steady quality of product; (2) can't produce in enormous quantities.
Also having Chinese patent application number is that 01217261.8 patent document discloses the technical scheme of " concentration gradient test strip ", it is characterized in that " being coated with microbiotic according to continuous concentration gradient on carrier, indicating the scale of its MIC simultaneously.Promptly antibiotic concentration is identical on horizontal arbitrary straight line, and on vertical arbitrary straight line, microbiotic is the chain index distribution.The drug distribution of this technical products is the concentration gradient that continuous stepless changes, but not the staged concentration gradient.From the angle of producing, this continuous concentration gradient medicaments insensitive detector bar, need in the length range about a 50mm, realization changes from the drug concentration of 256mg/ml to 0.016mg/ml, be that the concentration drop reaches 16000 times variation, and present production preparation and technology almost can't be carried out such production.Above-mentioned various reasons causes concentration gradient medicaments insensitive detector bar still can not extensively popularize so far and is used for routine clinical susceptibility detection, still can't all replace existing susceptibility detection method, as disk diffusion method, agar dilution, various robotization instrument mensuration etc.
Summary of the invention
At the above-mentioned shortcoming of prior art, the technical purpose that the present invention will reach be to provide a kind of steady quality that is easy to standardization and production in enormous quantities, product and easy to operate, measure accurate staged concentration gradient medicaments insensitive detector bar and preparation method thereof.
For this reason, technical scheme of the present invention is a kind of staged concentration gradient detector bar, this detector bar comprises rectangle sheet-like carrier and the medicine that is coated on the described carrier one side, described drug concentrations or dose along carrier shaft to presenting unidirectional Gradient distribution, be marked with the concentration numerical value of medicine on the kind of medicine and the correspondence position at the another side of described carrier, and the one side of described carrier etc. are divided longways several homalographic intervals are arranged, in each described homalographic interval, described relatively interval center is coated with the medicine piece of setting shape and area symmetrically; The dose of each medicine piece presents as the ladder variation of next or multistage promptly: the concentration difference of the medicine piece in different sections, but the coating thickness of each medicine piece of arranging in same section is identical with concentration, and the area size of each medicine piece of arranging in same section is according to the ladder ground increasing or decreasing that puts in order; On the another side of described bar shaped carrier, corresponding each homalographic interval, be marked with corresponding dose numerical value or the average of corresponding dose on interval volume.Laterally tell the homalographic interval of many equal portions on the rectangle carrier sheet of the present invention equably, because each interval area all equates, under the identical preceding topic of concentration, the thickness of painting medicine, according to the descending one-way order of spreading area, evenly be coated with the medicine of different area in the homalographic interval after, the dose of institute's painting medicine is directly proportional with the area of coating in the homalographic interval.With this further reckoning, in the homalographic interval in dose and this interval of coating the drug concentration on the unit volume be consistent.Thereby, realized that coated drug concentration is accurate staged concentration gradient distribution in each the homalographic interval on the rectangle loading bar.This precision staged concentration gradient medicaments insensitive detector bar has the design concept advanced person, is easy to advantages such as standardization, production in enormous quantities, controllable quality be effective, easy to use.
In order accurately to obtain each medicine piece by setting the area coating, the flat shape of each medicine piece is for calculating the different shape of area.The flat shape of described medicine piece is one of in rectangle, square, circle, ellipse, the prismatic.
In order to allow the medicine piece of smearing in the susceptibility testing process, to meet water disintegration immediately, release on interval volume, include the quick-release agent in the described medicine piece.Described quick-release agent is made up of in the following composition one or several: polysaccharide starch based, cellulose family, marine alga acids, potter's clay class, PVP class, gummy class.Described quick-release agent is selected from one or several in the following material: crospolyvinylpyrrolidone, Ac-Di-Sol, crosslinked carboxymethyl fecula sodium, microcrystalline cellulose, low-substituted hydroxypropyl cellulose.
Another technical scheme of the present invention is a kind of method for preparing aforesaid staged concentration gradient detector bar, and this method comprises the steps:
Making can bite coating medicine slurry big silk-screen template promptly: big silk-screen template region is divided into the little masterplate of a plurality of measure-alike elongated rectangular shape, again described little masterplate etc. is divided into several homalographic intervals longways, in each homalographic interval interval relatively center produce symmetrically a plurality of area sizes be one or the multistage stepped change the zone of biting;
Prepare the medicine slurry of one or more concentration so that carry out one or the ladder dose coating of multistage;
Described big silk-screen template is covered on the carrier sheet of corresponding size;
Axially make the rule of one-way Gradient distribution according to dose along little masterplate, adopt the medicine slurry of a certain compound concentration successively,, on described carrier sheet, be coated with through corresponding certain section the zone of biting;
All zones of biting all are coated with the carrier sheet that obtains behind the medicine slurry and carry out according to the border of little masterplate and size that cutting is cut, drying, vacuum-packed then.
Method of the present invention is diverted from one use to another the silk screen process technology in the coating medicine and is starched with preparation medicine ladder detector bar, and method advanced person is imbued with intention; The structure that comprises a plurality of little masterplates in the big silk-screen template, be convenient to adopt segmentation to hide the regional method of biting, medicine slurry with a kind of concentration scrapes a certain section the zone of biting that once just can be coated with all little masterplates, coating efficiency significantly improves, be suitable for producing in enormous quantities, and be easy to standardization and guarantee product quality.
In order more to give full play to the advantage of silk screen process technology, the present invention further arranges: the thickness in all zones of biting is identical.In big silk-screen template, the zone alignment mutually of biting that belongs to same stepped change section of the little masterplate of all elongated rectangular shape.Be arranged with 250 little templates of elongated rectangular shape in the described big silk-screen template.
Include the quick-release agent that following one or several one-tenth is grouped in the medicine slurry of the present invention: polysaccharide starch based, cellulose family, marine alga acids, potter's clay class, PVP class, gummy class.The component that can guarantee final products like this is reasonable, disintegration is rapid.
Explain technical scheme of the present invention in more detail below by embodiment and accompanying drawing.
Description of drawings
Fig. 1 is the structural representation of big silk-screen template embodiment of the present invention.
Fig. 2 is the front schematic view of staged concentration gradient detector bar embodiment of the present invention.
Fig. 3 is the schematic rear view of staged concentration gradient detector bar embodiment of the present invention.
Embodiment
Embodiment 1: as shown in Figure 1, 2, 3, at first on the big screen printing form 1 of a 305mm * 250mm, make 250 little masterplates 2 of identical elongated rectangular shape as follows: the little masterplate 2 of each elongated rectangular shape, long 61mm, wide 5mm, initial from the one end, in the length range of 52.2mm, horizontal even close branch and mark the white space 3 (seeing Fig. 2,3) of 29 equal portions, the area of each white space 3 all equates: L (1.8mm) * width W (5mm), masterplate 2 thickness H (0.3mm).In each five equilibrium white space 3 of masterplate 2, descending according to area, order label 1～29, by the zone 4 of biting for preparing rectangle or circle as shown in Table 1 in proper order respectively (ginseng Fig. 2,3),
Table one
Staged concentration gradient medicaments insensitive detector bar drug concentration and area, sequence number corresponding tables
| Sequence number | Unit volume drug concentration (mg/ml) | Region area (mm bites 2) | Long * wide (mm * mm) | Diameter (mm) |
| 1 | 256 | 9.000 | 1.80×5.00 | |
| 2 | 192 | 6.750 | 1.50×4.50 | |
| 3 | 128 | 4.500 | 1.00×4.50 | |
| 4 | 96 | 3.375 | 0.75×4.50 | |
| 5 | 64 | 2.250 | 1.693 | |
| 6 | 48 | 1.688 | 1.467 | |
| 7 | 32 | 1.125 | 1.197 | |
| 8 | 24 | 0.844 | 1.037 | |
| 9 | 16 | 0.563 | 0.846 | |
| 10 | 12 | 0.422 | 0.733 | |
| 11 | 8 | 0.281 | 0.599 | |
| 12 | 6 | 0.211 | 0.518 | |
| 13 | 4 | 0.141 | 0.423 |
| 14 | 3 | 0.105 | 0.367 | |
| 15 | 2 | 9.000 | 1.80×5.00 | |
| 16 | 1.5 | 6.750 | 1.50×4.50 | |
| 17 | 1.00 | 4.500 | 1.00×4.50 | |
| 18 | 0.75 | 3.375 | 0.75×4.5 | |
| 19 | 0.50 | 2.250 | 1.693 | |
| 20 | 0.38 | 1.688 | 1.467 | |
| 21 | 0.25 | 1.125 | 1.197 | |
| 22 | 0.19 | 0.844 | 1.037 | |
| 23 | 0.125 | 0.563 | 0.846 | |
| 24 | 0.094 | 0.422 | 0.733 | |
| 25 | 0.064 | 0.281 | 0.599 | |
| 26 | 0.047 | 0.211 | 0.518 | |
| 27 | 0.032 | 0.141 | 0.423 | |
| 28 | 0.023 | 0.105 | 0.367 | |
| 29 | 0.016 | 0.070 | 0.299 |
As No. 1 position region shape of biting is rectangle L
1(1.8mm) * W
1(5mm), the region area of biting is S
1(9mm
2), No. 2 position region shape of biting is rectangle L
2(1.5mm) * W
2(4.5mm), the region area of biting is S
2(6.75mm
2) ..., No. 12 region shape is bitten for circular in the position, and diameter is D
12(0.518mm), the region area of biting is S
12(0.211mm
2) ..., No. 15 position region shape of biting is rectangle L
15(1.8mm) * W
15(5mm), the region area of biting is S
15(9mm
2) ..., No. 22 region shape is bitten for circular, diameter D in the position
22(1.037mm), the region area of biting is S
2(0.844mm
2), the rest may be inferred, makes masterplate.The medicine of smearing of two kinds of concentration of preparation is starched, as concentration C
a(256mg/ml), C
bGentamicin (2mg/ml) is sneaked into 7% quick-release agent: Ac-Di-Sol (crosslinked CMC-Na) simultaneously in the medicine of these two kinds of concentration.The 15th～No. 29 zone 4 of biting on the big screen printing form 1 hidden, by big screen printing form 1, with concentration C
aMedicine slurry (256mg/ml) is applied on the plastic carrier thin slice 5, is formed on the per unit volume concentration C on the position No. 1
1Be 256mg/ml, the per unit volume concentration C of stairway degression on No. 14 position
14(calculate that specifically mode is as follows: in the 1st～No. 29 position, the spatial volume of each number position is identical, V=L (1.8mm) * W (5mm) * H (0.3mm)=2.7mm for the concentration gradient of 3mg/ml
3, the dose of coating is in each number position: the thickness H of the drug concentration C of painting medicine * painting medicine area S * painting medicine.As: the dose of coating is A in No. 1 position
1The drug concentration C of=painting medicine
a* painting medicine area S
1The thickness H of * painting medicine, the unit volume drug concentration C of No. 1 position
1=A
1/ V, the dose of coating is A in No. 2 position
2The drug concentration C of=painting medicine
a* painting medicine area S
2The thickness H of * painting medicine, the unit volume drug concentration in No. 2 position is C
2=A
2/ V, by that analogy.Be not difficult thus to extrapolate: C
1/ C
2C
14=A
1/ A
2A
14=S
1/ S
2S
14, C
15/ C
16C
29=A
15/ A
16A
29=S
15/ S
16S
29).Then, removing the covering of the 15th～No. 29 position on the masterplate, hide the 1st～No. 14 position on the masterplate, by big screen printing form 1, is C with concentration
bThe medicine of smearing (2mg/ml) is applied on the 15th～No. 29 position of plastic carrier thin slice 5, forms C
152mg/ml (No. 15 position)～C
29The concentration gradient of (0.016mg/ml No. 29 position).
Embodiment 2: shown in accompanying drawing 2,3, for above-mentioned plastic carrier thin slice 5 cuttings are cut, the accurate staged concentration gradient medicaments insensitive detector bar 6 of dry, elongated rectangular shape that vacuum packaging is made.In the length range of the 52.2mm of medicaments insensitive detector bar 6, formed the accurate staged concentration gradient of 256mg/ml (No. 1 position)～0.016mg/ml (No. 29 position).Be the back side of medicaments insensitive detector bar 6 in advance at plastic carrier thin slice 5, the back position of corresponding 256mg/ml (No. 1 position)～0.016mg/ml (No. 29 position) drug concentration, stamp 256,192,128,96,64,48 respectively ..., numeral such as 0.016, in 8.8mm white space 31, stamp the microbiotic code name, as the gentamicin code name is GM, and the penicillin code name is PG.
Claims (10)
1. staged concentration gradient detector bar, this detector bar comprises rectangle sheet-like carrier and the medicine that is coated on the described carrier one side, described drug concentrations or dose along carrier shaft to presenting unidirectional Gradient distribution, be marked with the concentration numerical value of medicine on the kind of medicine and the correspondence position at the another side of described carrier, it is characterized in that: the one side of described carrier etc. are divided longways several homalographic intervals, in each described homalographic interval, described relatively interval center is coated with the medicine piece of setting shape and area symmetrically; The dose of each medicine piece presents as the ladder variation of next or multistage promptly: the concentration difference of the medicine piece in different sections, but the coating thickness of each medicine piece of arranging in same section is identical with concentration, and the area size of each medicine piece of arranging in same section is according to the ladder ground increasing or decreasing that puts in order; On the another side of described bar shaped carrier, corresponding each homalographic interval, be marked with corresponding dose numerical value or the average of corresponding dose on interval volume.
2. staged concentration gradient detector bar as claimed in claim 1 is characterized in that: the flat shape of each medicine piece is for calculating the different shape of area.
3. staged concentration gradient detector bar as claimed in claim 1 or 2 is characterized in that: the flat shape of described medicine piece is one of in rectangle, square, circle, ellipse, the prismatic.
4. staged concentration gradient detector bar as claimed in claim 1 is characterized in that: include the quick-release agent in the described medicine piece.
5. staged concentration gradient detector bar as claimed in claim 4 is characterized in that: described quick-release agent is made up of in the following composition one or several: crospolyvinylpyrrolidone, Ac-Di-Sol, crosslinked carboxymethyl fecula sodium, microcrystalline cellulose, low-substituted hydroxypropyl cellulose.
6. method for preparing as claim 1,2,3, one of 4 or 5 staged concentration gradient detector bar, it is characterized in that: described method comprises the steps:
Making can bite coating medicine slurry big silk-screen template promptly: big silk-screen template region is divided into the little masterplate of a plurality of measure-alike elongated rectangular shape, again described little masterplate etc. is divided into several homalographic intervals longways, in each homalographic interval interval relatively center produce symmetrically a plurality of area sizes be one or the multistage stepped change the zone of biting;
Prepare the medicine slurry of one or more concentration so that carry out one or the ladder dose coating of multistage;
Described big silk-screen template is covered on the carrier sheet of corresponding size;
Axially make the rule of one-way Gradient distribution according to dose along little masterplate, adopt the medicine slurry of a certain compound concentration successively,, on described carrier sheet, be coated with through corresponding certain section the zone of biting;
All zones of biting all are coated with the carrier sheet that obtains behind the medicine slurry and carry out according to the border of little masterplate and size that cutting is cut, drying, vacuum-packed then.
7. the preparation method of staged concentration gradient detector bar as claimed in claim 6 is characterized in that: the thickness in all zones of biting is identical.
8. the preparation method of staged concentration gradient detector bar as claimed in claim 6 is characterized in that: in big silk-screen template, and the zone alignment mutually of biting that belongs to same stepped change section of the little masterplate of all elongated rectangular shape.
9. the preparation method of staged concentration gradient detector bar as claimed in claim 6 is characterized in that: include the quick-release agent that following one or several one-tenth is grouped in the described medicine slurry: crospolyvinylpyrrolidone, Ac-Di-Sol, crosslinked carboxymethyl fecula sodium, microcrystalline cellulose, low-substituted hydroxypropyl cellulose.
10. the preparation method of staged concentration gradient detector bar as claimed in claim 6 is characterized in that: be arranged with 250 little masterplates of elongated rectangular shape in the described big silk-screen template.
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|---|---|---|---|
| CNB2004100155704A CN100414298C (en) | 2004-03-08 | 2004-03-08 | Stepped concentration gradient detecting bar and preparing process thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100155704A CN100414298C (en) | 2004-03-08 | 2004-03-08 | Stepped concentration gradient detecting bar and preparing process thereof |
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| CN100414298C true CN100414298C (en) | 2008-08-27 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105021596B (en) * | 2014-04-18 | 2017-09-29 | 曾嵘斌 | Multilayer film dry chemical detection strip based on concentration gradient |
| WO2016134531A1 (en) | 2015-02-28 | 2016-09-01 | 曾嵘斌 | Dry chemical test strip with multiple layers of membranes based on concentration gradient |
| CN106872453B (en) * | 2017-01-11 | 2019-08-30 | 浙江大学 | Starch potassium iodide microchannel plastic film detects strip and preparation method and purposes |
| CN113845798A (en) * | 2021-09-15 | 2021-12-28 | 丹娜(天津)生物科技股份有限公司 | Antibacterial drug ink and method for preparing ester drug sensitive test strip by using same |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2194001Y (en) * | 1993-11-24 | 1995-04-05 | 中国人民解放军广州军区武汉总医院 | Gradient test paper for antibiotic sensitization test |
| US6140134A (en) * | 1996-10-29 | 2000-10-31 | Biocode, Inc. | Analyte detection with a gradient lateral flow device |
| CN2462387Y (en) * | 2001-01-21 | 2001-11-28 | 张同军 | Density gradient medicine sensitive test strip |
-
2004
- 2004-03-08 CN CNB2004100155704A patent/CN100414298C/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2194001Y (en) * | 1993-11-24 | 1995-04-05 | 中国人民解放军广州军区武汉总医院 | Gradient test paper for antibiotic sensitization test |
| US6140134A (en) * | 1996-10-29 | 2000-10-31 | Biocode, Inc. | Analyte detection with a gradient lateral flow device |
| CN2462387Y (en) * | 2001-01-21 | 2001-11-28 | 张同军 | Density gradient medicine sensitive test strip |
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| CN1667414A (en) | 2005-09-14 |
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