CN102373264A - Microbial detection method for antibacterial property of disposable hygienic product - Google Patents
Microbial detection method for antibacterial property of disposable hygienic product Download PDFInfo
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- CN102373264A CN102373264A CN2011102971054A CN201110297105A CN102373264A CN 102373264 A CN102373264 A CN 102373264A CN 2011102971054 A CN2011102971054 A CN 2011102971054A CN 201110297105 A CN201110297105 A CN 201110297105A CN 102373264 A CN102373264 A CN 102373264A
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Abstract
The invention discloses a microbial detection method for the antibacterial property of a disposable hygienic product. The microbial detection method comprises the following steps of: detecting bacterial microorganism and fungus microorganism. Simulative menses is prepared from a nutrient broth culture medium and a Sabouraud liquid culture medium respectively, nutritional substances contained in the simulative menses can be used for simulating human body blood to supply a good growing environment for bacteria, and an environment which is close to a human body is provided for a sample wafer, so that the application environment of the disposable hygienic product is better simulated, and the experimental accuracy is ensured.
Description
Technical field
The invention belongs to sanitary product detection method technical field, especially relate to the microorganism detection method of disposable sanitary articles bacterinertness.
Background technology
Showing according to research, contain rich nutrient substances in the menses, be " substratum " that bacterium multiplies without restraint, and the menses that accumulate on the sanitary towel improves perineal position humidity greatly, and the ventilation property variation more helps the breeding of bacterium.And common sanitary towel is after continuously using two hours, and the top layer total plate count can reach 107cfu/cm
2, sandwich layer is tens times of top layer.The women of some special positions perhaps night, can't accomplish just to change sanitary towel in two hours one time at all, and women's health condition is troubling.
Development along with antibiotic market; Antimicrobial form sanitary towel, protection pad are emerging in an endless stream on the market, and these antimicrobial form products adopt the spraying liquid antiseptic-germicide to reach the antimicrobial purpose mostly, but liquid preparation; The chemical ingredients of being added; Directly contact with human body, people's physique is different, possibly have allergic phenomena and produce.In view of the situation; Now occurred being added on the antiseptic-germicide of sandwich layer on the market, yet for this antiseptic-germicide of newly developing that is added into sandwich layer, GB does not also provide concrete detection method; Be necessary to research and develop a kind of method; This method is applicable to and detects the antibacterial effect that is added into sanitary towel sandwich layer antiseptic-germicide, through the service condition of simulation human body, objectively reflected the bacteriostasis property of product.
Summary of the invention
The technical problem that the present invention will solve provides a kind of microorganism detection method that can objectively respond the disposable sanitary articles bacterinertness.
The present invention provides a kind of microorganism detection method of disposable sanitary articles bacterinertness, may further comprise the steps:
1) detection of bacterial micro-organism
A, intestinal bacteria monitoring
A. prepare No. 1 and simulate menses, nutrient agar, PBS buffered soln, prepare test print and blank print;
B. prepare Escherichia coli bacteria liquid, bacterium liquid is joined simulate for No. 1 in the menses, the volume ratio of bacterium liquid and No. 1 simulation menses is 6.6 * 10
-7～6.7 * 10
-7, be mixed with the simulation menses that carry disease germs for No. 1;
C. on blank print, test print, add the simulation menses that carry disease germs for No. 1 respectively; Every print is put into plastics bag after folding; It is in 33 ℃～37 ℃ the biochemical incubator that plastics bag is put into set(ting)value; To test the taking-up of print, blank print to specified time, detect according to the detection method of total number of bacterial colony;
D. appearance liquid and the nutrient agar blended plate step c inoculated are put into 33 ℃～37 ℃ biochemical incubator, observations after 48 hours;
The detection of B, streptococcus aureus
A. prepare No. 1 and simulate menses, nutrient agar, PBS buffered soln, prepare test print and blank print;
B. prepare streptococcus aureus bacterium liquid, bacterium liquid is joined simulate for No. 1 in the menses, the volume ratio of bacterium liquid and No. 1 simulation menses is 6.6 * 10
-7～6.7 * 10
-7, be mixed with the simulation menses that carry disease germs for No. 1;
C. on blank print, test print, add the simulation menses that carry disease germs for No. 1 respectively; Every print is put into plastics bag after folding; It is in 33 ℃～37 ℃ the biochemical incubator that plastics bag is put into set(ting)value; To test the taking-up of print, blank print to specified time, detect according to the detection method of total number of bacterial colony;
D. appearance liquid and the nutrient agar blended plate step c inoculated are put into 33 ℃～37 ℃ biochemical incubator, observations after 48 hours;
2) detection of fungi microbe
A. prepare No. 2 and simulate menses, sabouraud's agar, saline water, PBS buffered soln, prepare test sample and blank print;
B. preparing Candida albicans bacterium liquid, is 6.6 * 10 with the volume ratio of bacterium liquid and No. 2 simulation menses
-7～6.7 * 10
-7, be mixed with the simulation menses that carry disease germs for No. 2;
C. on blank print, test print, add the simulation menses that carry disease germs for No. 2 respectively; Every print is put into plastics bag after folding; It is in 33 ℃～37 ℃ the biochemical incubator that plastics bag is put into set(ting)value; To test the taking-up of print, blank print to specified time, detect according to the detection method of total number of bacterial colony;
D. appearance liquid and the sabouraud's agar blended plate the c step inoculated are put into 33 ℃～37 ℃ biochemical incubator, observations after 72 hours.
Preferably; Said No. 1 simulation menses are nutrient broth medium; Its compound method is following, and wherein the amount of each raw material is parts by weight: 10 parts of peptones, 3 parts of beef extract powders, sodium-chlor are joined in 1000 parts of zero(ppm) water for 5 parts, above-mentioned solution was carried out autoclaving 15 minutes under 121 ℃).
Preferably; Said No. 2 simulation menses are the Sha Shi liquid nutrient medium; Its compound method is following, and wherein the amount of each raw material is parts by weight: 10 parts of peptones, glucose are joined in 1000 parts of zero(ppm) water for 40 parts, above-mentioned solution was carried out autoclaving 20 minutes under 115 ℃.
Preferably; Said nutrient agar; Its compound method is following, and wherein the amount of each raw material is parts by weight: get 10 parts of peptones, 3 parts of beef extract powders, 5 parts in sodium-chlor, agar and join in 1000 parts of zero(ppm) water for 14 parts, above-mentioned solution was carried out autoclaving 15 minutes at 121 ℃.
Preferably, said PBS buffered soln, its compound method is following, wherein the amount of each raw material is parts by weight: get 2.83 parts of ADSPs, potassium primary phosphate joins in 1000 parts of zero(ppm) water for 1.36 parts, with above-mentioned solution 121 ℃ of autoclavings 15 minutes.
Preferably, said sabouraud's agar, its compound method is following, wherein the amount of each raw material is parts by weight: 10 parts of peptones, 40 parts of glucose, agar join in 1000 parts of zero(ppm) water for 20 parts, with above-mentioned solution 115 ℃ of autoclavings 20 minutes.
Said Escherichia coli bacteria liquid, streptococcus aureus are respectively intestinal bacteria, streptococcus aureus to be added in the PBS damping fluid, and the recovery bacterium number of the bacterium colony of two kinds of bacterium liquid is 1 * 10
4Cfu/ml～9 * 10
4Cfu/ml.
Preferably, said bacterium liquid is that Candida albicans bacterium liquid is joined in the PBS damping fluid, and the recovery bacterium number of bacterium colony is 1 * 10
4Cfu/ml～9 * 10
4Cfu/ml.
Advantage of the present invention and effect: reasonably menses are simulated in preparation; The nutritive substance that contains in the simulation menses can be simulated blood of human body and offered the good growing environment of bacterium; And give the environment of print near human body; Better the applied environment of simulation disposable sanitary articles has guaranteed the accuracy of testing.
Embodiment
In order to understand the present invention, through concrete embodiment the present invention is described further below.
Embodiment one
The microorganism detection method of disposable sanitary articles bacterinertness may further comprise the steps:
1) detection of bacterial micro-organism
A, colibacillary detection
A. simulate menses No. 1 with the nutrient broth medium preparation, and preparation nutrient agar, PBS damping fluid and saline water, test print and blank print prepared;
The process for preparation of No. 1 simulation menses is following:
Get peptone 10g, beef extract powder 3g, sodium-chlor 5g and join in the 1L zero(ppm) water, subsequent use 121 ℃ of following autoclavings 15 minutes;
The process for preparation of nutrient agar is following:
Get peptone 10g, beef extract powder 3g, sodium-chlor 5g, agar 14g and join in the 1L zero(ppm) water, 121 ℃ of autoclavings 15 minutes;
The process for preparation of PBS damping fluid is following:
Get ADSP 2.83g, potassium primary phosphate 1.36g joins in the 1L zero(ppm) water, and is subsequent use 121 ℃ of autoclavings 15 minutes.
B. prepare intestinal bacteria, intestinal bacteria are added in the PBS damping fluid, the recovery bacterium number of bacterium colony is 1 * 10
4Cfu/ml～9 * 10
4Cfu/ml gets above-mentioned Escherichia coli bacteria liquid 10 μ l then and joins in No. 1 simulation of the 15ml menses, is mixed with the simulation menses that carry disease germs for No. 1;
C. add the 15ml simulation menses that carry disease germs for No. 1 on the print respectively in blank print, test; Every print is put into plastics bag after folding; It is in 33 ℃～37 ℃ the biochemical incubator that plastics bag is put into set(ting)value; To test the taking-up of print, blank print to specified time, detect according to the detection method of total number of bacterial colony;
D. appearance liquid and the sabouraud's agar blended plate the c step inoculated are put into 33 ℃～37 ℃ biochemical incubator, observe the plate cultivation results after 48 hours;
The detection of B, streptococcus aureus
A. simulate menses No. 1 with the nutrient broth medium preparation, and preparation nutrient agar, PBS damping fluid and saline water, test print and blank print prepared;
The process for preparation of No. 1 simulation menses is following:
Get peptone 10g, beef extract powder 3g, sodium-chlor 5g and join in the 1L zero(ppm) water, subsequent use 121 ℃ of following autoclavings 15 minutes;
The process for preparation of nutrient agar is following:
Get peptone 10g, beef extract powder 3g, sodium-chlor 5g, agar 14g and join in the 1L zero(ppm) water, 121 ℃ of autoclavings 15 minutes;
The process for preparation of PBS damping fluid is following:
Get ADSP 2.83g, potassium primary phosphate 1.36g joins in the 1L zero(ppm) water, and is subsequent use 121 ℃ of autoclavings 15 minutes.
B. prepare streptococcus aureus bacterium liquid, streptococcus aureus is added in the PBS damping fluid, the recovery bacterium number of bacterium colony is 1 * 10
4Cfu/ml～9 * 10
4Cfu/ml gets above-mentioned streptococcus aureus bacterium liquid 10 μ l then and joins in No. 1 simulation of the 15ml menses, is mixed with the simulation menses that carry disease germs for No. 1;
C. add the 15ml simulation menses that carry disease germs for No. 1 on the print respectively in blank print, test; Every print is put into plastics bag after folding; It is in 33 ℃～37 ℃ the biochemical incubator that plastics bag is put into set(ting)value; To test the taking-up of print, blank print to specified time, detect according to the detection method of total number of bacterial colony;
D. appearance liquid and the sabouraud's agar blended plate the c step inoculated are put into 33 ℃～37 ℃ biochemical incubator, observe the plate cultivation results after 48 hours;
2) detection of fungi microbe
A. with No. 2 simulations of Sha Shi liquid nutrient medium preparation menses, the preparation sabouraud's agar is prepared test sample and blank print;
The process for preparation of No. 2 simulation menses is following:
Get peptone 10g, glucose 40g joins in the 1L zero(ppm) water, 115 ℃ of autoclavings 20 minutes are subsequent use;
The process for preparation of sabouraud's agar is following:
It is subsequent use that peptone 10g, glucose 40g, agar 20g join in the 1L zero(ppm) water 115 ℃ of autoclavings 20 minutes;
B. prepare Candida albicans bacterium liquid, Candida albicans is joined in the PBS damping fluid, the PBS damping fluid that used PBS damping fluid and step 1) are used is a kind of, and the recovery bacterium number of bacterium colony is 1 * 10
4Cfu/ml～9 * 10
4Cfu/ml.Above-mentioned bacterium liquid is got 10 μ l join in No. 2 simulations of the 15ml menses, be mixed with the simulation menses that carry disease germs for No. 2;
C. add the 15ml simulation menses that carry disease germs for No. 2 on the print respectively in blank print, test; Every print is put into plastics bag after folding; It is in 33 ℃～37 ℃ the biochemical incubator that plastics bag is put into set(ting)value; To test the taking-up of print, blank print to specified time, detect according to the detection method of total number of bacterial colony;
D. appearance liquid and the sabouraud's agar blended plate step c inoculated are put into 33 ℃～37 ℃ biochemical incubator, observe the plate cultivation results after 72 hours.
The method of evaluation of result: the detection method according to the GB15979-2002 total number of bacterial colony detects
1), the result of bacterial micro-organism calculates by following formula:
In the formula: X is the sample total number of bacterial colonies, cfu/g; N is a total number of bacterial colonies on 5 flat boards; K is an extent of dilution.
2), the following formula of ammonium as a result of fungi microbe calculates
In the formula: X is a bacteriostasis rate, %; Nc is the average colony count of control sample, cfu/g; Ns is by the average colony count of test agent, cfu/g.
The present invention is not limited to above-mentioned preferred forms, and other any identical with the present invention or akin products that anyone draws under enlightenment of the present invention all drop within protection scope of the present invention.
Claims (8)
1. the microorganism detection method of disposable sanitary articles bacterinertness is characterized in that: may further comprise the steps:
1) detection of bacterial micro-organism
A, intestinal bacteria monitoring
A. prepare No. 1 and simulate menses, nutrient agar, PBS buffered soln, prepare test print and blank print;
B. prepare Escherichia coli bacteria liquid, bacterium liquid is joined simulate for No. 1 in the menses, the volume ratio of bacterium liquid and No. 1 simulation menses is 6.6 * 10
-7～6.7 * 10
-7, be mixed with the simulation menses that carry disease germs for No. 1;
C. on blank print, test print, add the simulation menses that carry disease germs for No. 1 respectively; Every print is put into plastics bag after folding; It is in 33 ℃～37 ℃ the biochemical incubator that plastics bag is put into set(ting)value; To test the taking-up of print, blank print to specified time, detect according to the detection method of total number of bacterial colony;
D. appearance liquid and the nutrient agar blended plate step c inoculated are put into 33 ℃～37 ℃ biochemical incubator, observations after 48 hours;
The detection of B, streptococcus aureus
A. prepare No. 1 and simulate menses, nutrient agar, PBS buffered soln, prepare test print and blank print;
B. prepare streptococcus aureus bacterium liquid, bacterium liquid is joined simulate for No. 1 in the menses, the volume ratio of bacterium liquid and No. 1 simulation menses is 6.6 * 10
-7～6.7 * 10
-7, be mixed with the simulation menses that carry disease germs for No. 1;
C. on blank print, test print, add the simulation menses that carry disease germs for No. 1 respectively; Every print is put into plastics bag after folding; It is in 33 ℃～37 ℃ the biochemical incubator that plastics bag is put into set(ting)value; To test the taking-up of print, blank print to specified time, detect according to the detection method of total number of bacterial colony;
D. appearance liquid and the nutrient agar blended plate step c inoculated are put into 33 ℃～37 ℃ biochemical incubator, observations after 48 hours;
2) detection of fungi microbe
A. prepare No. 2 and simulate menses, sabouraud's agar, saline water, PBS buffered soln, prepare test sample and blank print;
B. preparing Candida albicans bacterium liquid, is 6.6 * 10 with the volume ratio of bacterium liquid and No. 2 simulation menses
-7～6.7 * 10
-7, be mixed with the simulation menses that carry disease germs for No. 2;
C. on blank print, test print, add the simulation menses that carry disease germs for No. 2 respectively; Every print is put into plastics bag after folding; It is in 33 ℃～37 ℃ the biochemical incubator that plastics bag is put into set(ting)value; To test the taking-up of print, blank print to specified time, detect according to the detection method of total number of bacterial colony;
D. appearance liquid and the sabouraud's agar blended plate the c step inoculated are put into 33 ℃～37 ℃ biochemical incubator, observations after 72 hours.
2. the microorganism detection method of disposable sanitary articles bacterinertness as claimed in claim 1; It is characterized in that: said No. 1 simulation menses are nutrient broth medium; Its compound method is following; Wherein the amount of each raw material is parts by weight: 10 parts of peptones, 3 parts of beef extract powders, sodium-chlor are joined in 1000 parts of zero(ppm) water for 5 parts, above-mentioned solution was carried out autoclaving 15 minutes under 121 ℃).
3. the microorganism detection method of disposable sanitary articles bacterinertness as claimed in claim 1; It is characterized in that: said No. 2 simulation menses are the Sha Shi liquid nutrient medium; Its compound method is following; Wherein the amount of each raw material is parts by weight: 10 parts of peptones, glucose are joined in 1000 parts of zero(ppm) water for 40 parts, above-mentioned solution was carried out autoclaving 20 minutes under 115 ℃.
4. the microorganism detection method of disposable sanitary articles bacterinertness as claimed in claim 1; It is characterized in that: said nutrient agar; Its compound method is following; Wherein the amount of each raw material is parts by weight: get 10 parts of peptones, 3 parts of beef extract powders, 5 parts in sodium-chlor, agar and join in 1000 parts of zero(ppm) water for 14 parts, above-mentioned solution was carried out autoclaving 15 minutes at 121 ℃.
5. the microorganism detection method of disposable sanitary articles bacterinertness as claimed in claim 1; It is characterized in that: said PBS buffered soln; Its compound method is following; Wherein the amount of each raw material is parts by weight: get 2.83 parts of ADSPs, potassium primary phosphate joins in 1000 parts of zero(ppm) water for 1.36 parts, with above-mentioned solution 121 ℃ of autoclavings 15 minutes.
6. the microorganism detection method of disposable sanitary articles bacterinertness as claimed in claim 1; It is characterized in that: said sabouraud's agar; Its compound method is following; Wherein the amount of each raw material is parts by weight: 10 parts of peptones, 40 parts of glucose, agar join in 1000 parts of zero(ppm) water for 20 parts, with above-mentioned solution 115 ℃ of autoclavings 20 minutes.
7. the microorganism detection method of disposable sanitary articles bacterinertness as claimed in claim 1; It is characterized in that: said Escherichia coli bacteria liquid, streptococcus aureus are respectively intestinal bacteria, streptococcus aureus to be added in the PBS damping fluid, and the recovery bacterium number of the bacterium colony of two kinds of bacterium liquid is 1 * 10
4Cfu/ml～9 * 10
4Cfu/ml.
8. the microorganism detection method of disposable sanitary articles bacterinertness as claimed in claim 1 is characterized in that: said bacterium liquid is that Candida albicans bacterium liquid is joined in the PBS damping fluid, and the recovery bacterium number of bacterium colony is 1 * 10
4Cfu/ml～9 * 10
4Cfu/ml.
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| CN2011102971054A CN102373264A (en) | 2011-09-30 | 2011-09-30 | Microbial detection method for antibacterial property of disposable hygienic product |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104020009A (en) * | 2013-03-01 | 2014-09-03 | 天津出入境检验检疫局工业产品安全技术中心 | Disposable hygienic product treatment device |
| CN109490186A (en) * | 2018-12-20 | 2019-03-19 | 扬州倍加洁日化有限公司 | A kind of validity period measuring method behind wet tissue product Kaifeng |
| CN109609590A (en) * | 2018-12-21 | 2019-04-12 | 扬州倍加洁日化有限公司 | A kind of anti-microbial property test method of wet tissue product |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1670047A (en) * | 2004-12-31 | 2005-09-21 | 仲恺农业技术学院 | Antibacterial high water-absorbent resin and method for preparation thereof |
-
2011
- 2011-09-30 CN CN2011102971054A patent/CN102373264A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1670047A (en) * | 2004-12-31 | 2005-09-21 | 仲恺农业技术学院 | Antibacterial high water-absorbent resin and method for preparation thereof |
Non-Patent Citations (2)
| Title |
|---|
| 沈伟 等: "附录C——产品杀菌性能、抑菌性能与稳定性测试方法", 《GB 15979-2002——一次性使用卫生用品卫生标准》, 1 September 2002 (2002-09-01), pages 663 - 665 * |
| 隆泉 等: "抗菌织物的抗菌性快速检测方法研究：奎因法的改良", 《上海纺织技术》, vol. 35, no. 4, 18 April 2007 (2007-04-18) * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104020009A (en) * | 2013-03-01 | 2014-09-03 | 天津出入境检验检疫局工业产品安全技术中心 | Disposable hygienic product treatment device |
| CN104020009B (en) * | 2013-03-01 | 2016-03-23 | 天津出入境检验检疫局工业产品安全技术中心 | Disposable sanitary articles treating apparatus |
| CN109490186A (en) * | 2018-12-20 | 2019-03-19 | 扬州倍加洁日化有限公司 | A kind of validity period measuring method behind wet tissue product Kaifeng |
| CN109609590A (en) * | 2018-12-21 | 2019-04-12 | 扬州倍加洁日化有限公司 | A kind of anti-microbial property test method of wet tissue product |
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