CN201089771Y - Count culture flat for anaerobium and viable bacteria - Google Patents
Count culture flat for anaerobium and viable bacteria Download PDFInfo
- Publication number
- CN201089771Y CN201089771Y CNU2007200802440U CN200720080244U CN201089771Y CN 201089771 Y CN201089771 Y CN 201089771Y CN U2007200802440 U CNU2007200802440 U CN U2007200802440U CN 200720080244 U CN200720080244 U CN 200720080244U CN 201089771 Y CN201089771 Y CN 201089771Y
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- anerobe
- culture plate
- hard transparent
- bacterial count
- live bacterial
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Abstract
An anaerobe viable counting culture plate is characterized in that: a rectangular sheet box with small height is made of hard transparent material, the long ends of the box are provided with openings, and seal closures are arranged on the openings. The anaerobe viable counting culture plate of the utility model which has correct counting results, simple structure, low cost and simple operation neither needs any peripheral equipment nor needs air pumping and charging or other chemical deoxidization measures, thereby having a wide application range.
Description
Technical field
The utility model belongs to cultivates anerobe viable bacteria, the board device when particularly the anerobe viable bacteria being counted cultivation.
Background technology
Have at least two kinds of situations need make the anerobe live bacterial count: a kind of situation is the quality monitoring of effective microorganism preparation, as determining living bacteria count in the photosynthetic bacterium microbial inoculum, detecting lactobacillus-fermented product viable bacteria content etc.; Another kind of situation is to the harmful microbe monitoring, as detecting the sulphite reduction clostridium in carbohydrate, starch materials or vegetables, the tea products.No matter be which kind of situation, be to adopt certain peripheral unit such as anaerobic jar or anaerobism bag to the general way of anerobe live bacterial count at present, also can outside culture dish, take certain deoxygenation measure, as reacting deoxygenation with chemical reagent or vacuumizing again inflated with nitrogen or carbon dioxide deoxygenation etc., but these way costs are higher and the operation is more loaded down with trivial details, when running into the situation that needs illumination cultivation (as the cultivation of photosynthetic bacterium), just more incompatible.
Studies show that: adopting traditional culture dish is anaerobic bacteria culture complex operation and the expensive major cause of required equipment, and culture dish surface area commonly used is bigger, and diameter is that the surface-area of the culture dish of 9cm is 23.4cm
2, substratum thickness is usually less than 0.5cm, and whole substratum and air contact surface are very big, and oxygen molecule can penetrate substratum easily, has influenced the accuracy of counting, and therefore normal peripherals and the interior rear oxygen of eliminating equipment of using can be cultivated anerobe.The area that the minimizing substratum contacts with air, the thickness that increases substratum all can more easily obtain anaerobic condition.Liu Jun justice etc. has been studied a kind of semi-solid test tube and is counted and cultivate photosynthetic bacterium (" method of counting of photosynthetic bacterium-semi-solid test tube method " " Chinese aquatic products " 2003,11.P68～69) for this reason.But because test tube is a right cylinder, certain thickness is arranged, regular meeting restatement occurs or leaks the meter phenomenon when enumeration.
Summary of the invention
The technical problems to be solved in the utility model provides a kind of result anerobe live bacterial count culture apparatus accurate, simple in structure, with low cost, easy and simple to handle.
The utility model solves the problems of the technologies described above with following technical scheme: make highly very little rectangle sheet cassette with hard transparent material, a long end opening of box, mouthful outer seal closure that is equipped with.
Hard transparent material is glass or synthetic glass or hard transparent plastic.
Anerobe live bacterial count culture plate count results of the present utility model is accurate, simple in structure, has only plate body and seal closure two portions; Equipment and use cost are cheap, except that flat board, need not any peripherals such as anaerobic jar or anaerobism bag etc.; Easy and simple to handle, need not to bleed and inflate or take other chemical deoxidization measure; Applied range except that cultivating conventional anerobe, also can be cultivated the anerobe that needs illumination, as photosynthetic bacterium.
Description of drawings
Fig. 1 is the structural representation of the utility model anerobe live bacterial count culture plate.
Embodiment
Make highly very little rectangle sheet cassette with hard transparent material, for example glass or synthetic glass or hard transparent plastic thin plate, a long end opening of box 1, mouthful outer seal closure 2 that is equipped with.Box body can be made the rectangular parallelepiped of long 9cm～10cm, wide 5cm～6cm, thick 0.4cm～0.6cm, only stays an opening at a long end, makes the seal closure that can seal opening simultaneously, and seal closure can be 1.2cm～1.5cm deeply.Sheet gauge 1～2mm.
Below be several embodiment of the size and the behaviour in service of anerobe live bacterial count culture plate of the present utility model:
Embodiment 1: the interior cavity length of anerobe live bacterial count culture plate plate body is 9cm, and width is 6cm, and thickness is 0.5cm, and cumulative volume is 27cm
3, port area is 3cm
2The inner chamber degree of depth of seal closure (being the degree of depth that the plate body can insert seal closure) is 1.4cm, and width is 6.1cm, and thickness is 0.6cm.When cultivating the counting photosynthetic bacterium, get the 1mL testing sample earlier and place dull and stereotyped bottom, again with in the disposable adding flat board of 20mL substratum, treat culture medium solidifying after, add 5mL 2% pure agar again and cover, cover seal closure at last, put the cultivation illumination condition thermophilic under.
Embodiment 2: the interior cavity length of anerobe live bacterial count culture plate plate body is 10cm, and width is 6cm, and thickness is 0.5cm, and cumulative volume is 30cm
3, port area is 3cm
2The inner chamber degree of depth of seal closure is 1.5cm, and width is 6.1cm, and thickness is 0.6cm.When cultivating the counting lactobacillus, get the 1mL testing sample earlier and place dull and stereotyped bottom, again with in the disposable adding flat board of 20mL substratum, treat culture medium solidifying after, add 5mL 2% pure agar again and cover, cover the plate lid at last, put in the incubator and cultivate.
Embodiment 3: the interior cavity length of anerobe live bacterial count culture plate plate body is 9cm, and width is 5.6cm, and thickness is 0.5cm, and cumulative volume is 25cm
3, port area is 2.8cm
2The inner chamber degree of depth of seal closure is 1.2cm, and width is 5.7cm, and thickness is 0.6cm.When cultivating counting sulphite reduction clostridium, get the 1mL testing sample earlier and place dull and stereotyped bottom, again with in the disposable adding flat board of 20mL substratum, treat culture medium solidifying after, add 4mL 2% pure agar again and cover, cover the plate lid at last, put in the incubator and cultivate.
Claims (2)
1. an anerobe live bacterial count culture plate is characterized in that making highly very little rectangle sheet cassette with hard transparent material, a long end opening of box, mouthful outer seal closure that is equipped with.
2. anerobe live bacterial count culture plate as claimed in claim 1 is characterized in that hard transparent material is glass or synthetic glass or hard transparent plastic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007200802440U CN201089771Y (en) | 2007-07-05 | 2007-07-05 | Count culture flat for anaerobium and viable bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007200802440U CN201089771Y (en) | 2007-07-05 | 2007-07-05 | Count culture flat for anaerobium and viable bacteria |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201089771Y true CN201089771Y (en) | 2008-07-23 |
Family
ID=39861530
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2007200802440U Expired - Fee Related CN201089771Y (en) | 2007-07-05 | 2007-07-05 | Count culture flat for anaerobium and viable bacteria |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201089771Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103805676A (en) * | 2014-02-14 | 2014-05-21 | 青岛农业大学 | Simple and quick cultivation and counting method for anaerobic bacteria |
| CN105087445A (en) * | 2015-09-07 | 2015-11-25 | 杭州市农业科学研究院 | Method for isolating and purifying photosynthetic bacteria through AnaeroPack |
| CN107164222A (en) * | 2017-06-22 | 2017-09-15 | 成都远洋蓝猫科技有限公司 | Sample process box for Bacteria Detection |
-
2007
- 2007-07-05 CN CNU2007200802440U patent/CN201089771Y/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103805676A (en) * | 2014-02-14 | 2014-05-21 | 青岛农业大学 | Simple and quick cultivation and counting method for anaerobic bacteria |
| CN105087445A (en) * | 2015-09-07 | 2015-11-25 | 杭州市农业科学研究院 | Method for isolating and purifying photosynthetic bacteria through AnaeroPack |
| CN107164222A (en) * | 2017-06-22 | 2017-09-15 | 成都远洋蓝猫科技有限公司 | Sample process box for Bacteria Detection |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080723 |