CN204874519U - Culture dish is used to volatile materials bacteriostatic activity survey - Google Patents
Culture dish is used to volatile materials bacteriostatic activity survey Download PDFInfo
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- CN204874519U CN204874519U CN201520520229.8U CN201520520229U CN204874519U CN 204874519 U CN204874519 U CN 204874519U CN 201520520229 U CN201520520229 U CN 201520520229U CN 204874519 U CN204874519 U CN 204874519U
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- ware
- diapire
- culture dish
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- wall
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Abstract
The utility model discloses a culture dish is used to volatile materials bacteriostatic activity survey, include: at the bottom of the ware, ware lid and seal ring wherein include at the bottom of diapire at the bottom of the ware, the ware inside wall at the bottom of lateral wall and the ware at the bottom of the ware, and the ware lid play sealed effect including ware lid diapire, ware lid lateral wall, ware lid inside wall, seal ring embedding at the annular groove inslot of ware lid. The utility model provides a special culture dish of volatile materials bacteriostatic activity survey is applicable to that different growth rate indicator bacterias are convenient measures, has improved experimental efficiency, has reduced the experimental error that measuring tool brought, has improved the leakproofness in the ware, and easy and simple to handle, the degree of accuracy that has improved bacteriostatic activity analysis results.
Description
Technical field
The utility model belongs to Bioexperiment apparatus field, relates to a kind of culture dish, particularly the Antibacterial Activity special culture dish of volatile matter.
Background technology
Culture dish is a kind of vessel for microorganism or cell cultures, by the bottom of the ware of flat circle plate-like and ware lid form.In current research, the method for measuring plant and microbial source Identification of Volatile Fungistatic Compounds bacteriostatic activity mainly contains make-up method at the bottom of ware and two lattice plate opposite culture methods.
Make-up method at the bottom of ware is by make-up at the bottom of two Nostoc commune Vanch wares, and twine envelope with sealed membrane, but this method is high to the specification of quality coherence request at the bottom of two wares used, and complex operation step, also can produce crossed contamination simultaneously, reduce the accuracy of Antibacterial Activity result.
Two lattice plate opposite culture methods adopt two special sectional culture dishs, namely with a dividing plate, culture dish is divided into two semicircle shape regions, access indicator and volatile matter to be measured or antagonistic microbe respectively, but there is a lot of shortcoming in this method: make the spatial dimension for the growth of target indicator reduce by half, and due to this scope be semicircle, limit the growth of some growth and breeding speed indicator especially; This custom-made Petri dish poor sealing simultaneously, volatile matter can be diffused into outside ware, and experimental result error is also larger.
And these two kinds of methods all adopt the surveying instruments such as vernier callipers after experiment terminates, read the diameter of inoculation indicator, such that experimentation is loaded down with trivial details consumes the plenty of time, reduce conventional efficient.
Utility model content
In order to overcome the deficiency of art methods, the purpose of this utility model be to provide a kind of measure easy, simple to operate, accuracy is high, indicator growth scope wide, the volatile matter Antibacterial Activity special culture dish of good airproof performance.
For achieving the above object, the utility model employing technical scheme is:
A kind of volatile matter Antibacterial Activity culture dish, comprising: at the bottom of ware, ware lid, seal washer, in order at the bottom of ware and ware lid can seal and connect, described seal washer is embedded in ware lid annular ditch groove.
Comprise at the bottom of described ware: inner side-wall at the bottom of outer side wall, ware at the bottom of diapire, ware at the bottom of ware, diapire at the bottom of described ware has measures mark, and at the bottom of described ware, outer side wall is greater than inner side-wall at the bottom of ware, is all vertically connected at diapire at the bottom of ware.By inner side-wall at the bottom of ware and outer side wall, diapire at the bottom of ware is divided into two regions to widen the growth scope of indicator.
Further, diapire at the bottom of described ware is circle, and its center of circle has annular vaccination to mark.
Further, described measurement is designated scale marker, and scale marker can be concentric scale ring, by this structure for measuring the diameter of indicator.
Further, described concentric scale ring extends out to inner side-wall at the bottom of ware from the diapire center of circle at the bottom of ware.
Further, outer side wall at the bottom of described ware is ring-type, and diameter equals diapire diameter at the bottom of ware.
Further, inner side-wall at the bottom of described ware is ring-type, and diameter is less than diapire diameter at the bottom of ware, by this structure object, height, lower than outer side wall at the bottom of ware, is that the generation that contacts with indicator enabling volatile matter good is reacted.
Further, test substance region and indicator region is divided at the bottom of described ware.
Further, described test substance region is the annular region at the bottom of ware at the bottom of outer side wall to ware between inner side-wall, and indicator region is the border circular areas at the bottom of ware at the bottom of inner side-wall to ware between the diapire center of circle.
Further, described ware lid, comprising:
Ware lid diapire, be circle, diameter is comparatively greater than diapire at the bottom of ware;
Ware lid outer side wall is ring-type, and diameter equals ware lid diapire diameter, is highly less than outer side wall at the bottom of ware, is vertically connected at ware lid diapire;
Ware lid inner side-wall is ring-type, and diameter is smaller than ware lid diapire diameter, is highly less than outer side wall at the bottom of ware, is vertically connected at ware lid diapire.
Volatile matter Antibacterial Activity culture dish of the present utility model can be made with transparent material, as glass, PC or transparent ABS etc. are processed.
Seal washer in volatile matter Antibacterial Activity culture dish of the present utility model selects high temperature resistant, corrosion resistant elastomeric material to make, and urethane, chloroprene rubber, silicon rubber etc. can be selected to process.
The utility model is volatile matter Antibacterial Activity culture dish, adopts the beneficial effect of the technical program:
1. concentric scale ring is carved with in culture dish diapire bottom, directly can read the indicator diameter of inoculation, make measuring process convenient and swift, improve conventional efficient, reduce the experimental error that survey instrument brings simultaneously, improves the tolerance range of experimental data;
2. cultivation region at the bottom of ware is divided into two regions by inner side-wall at the bottom of ware, and the border circular areas in inner side-wall at the bottom of ware is indicator inoculation region, for indicator provides wider growing space, is applicable to different speed of growth indicator;
3. utilize seal washer can by compact siro spinning technology at the bottom of ware lid and ware, after test inoculation has operated, on ware cover buckle is at the bottom of ware, pressing can cover tightly, simplify experimental implementation process, and improve the stopping property of culture dish significantly, the stopping property of culture dish improves, avoid volatile matter in experimentation to ware external diffusion, improve the accuracy of Antibacterial Activity result.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model;
Fig. 2 is cross-sectional view of the present utility model;
Fig. 3 is upward view at the bottom of ware of the present utility model;
Fig. 4 is ware lid upward view of the present utility model;
Wherein, 1 is ware lid, and 2 is seal washers, 3 is at the bottom of ware, and 4 is diapires at the bottom of ware, and 5 is outer side walls at the bottom of ware, 6 is inner side-walls at the bottom of ware, and 7 is ware lid diapires, and 8 is ware lid outer side walls, 9 is ware lid inner side-walls, 10 is annular ditch grooves, and 11 is vaccinations, and 12 is concentric scale rings, 13 indicator regions, 14 test substance regions.
Embodiment
In order to make the object of utility model, technical scheme and advantage clearly, below in conjunction with accompanying drawing, the utility model is further elaborated.
See Fig. 1, a kind of volatile matter Antibacterial Activity culture dish, comprising:
At the bottom of ware 1, ware lid 3, seal washer 2, seal washer 2 is embedded in ware lid 3 annular ditch groove 10.
Described in the present embodiment at the bottom of ware 1, as shown in Figure 2,3, comprising:
Diapire 4 at the bottom of ware, for circle, diameter 90mm, thickness 1.5mm, its center of circle has annular vaccination 11 to mark, the concentric scale ring 12 that at the bottom of ware, it is the center of circle that diapire 4 bottom has with vaccination 11, described concentric scale ring 12 extends out to inner side-wall 6 at the bottom of ware from diapire 4 center of circle at the bottom of ware, and minimum scale unit is 1mm and has graduated scale at 8 bearing marks;
Outer side wall 5 at the bottom of ware is ring-type, and diameter equals diapire 4 diameter at the bottom of ware, height 20mm, thickness 1.5mm, and is vertically connected at diapire 4 at the bottom of ware;
Inner side-wall 6 at the bottom of ware is ring-type, and diameter is less than diapire 4 diameter at the bottom of ware, and height gets 15mm lower than outer side wall at the bottom of ware 5, thickness 1.5mm, and is vertically connected at diapire 4 at the bottom of ware, inner side-wall 6 at the bottom of ware apart from vaccination 11 apart from being 30mm.
Outer side wall 5 at the bottom of ware and inner side-wall 6 at the bottom of ware are all connected to diapire 4 at the bottom of ware and 1 will be divided into two regions at the bottom of ware, be respectively test substance region 14 and indicator region 13, described test substance region 14 is the annular region at the bottom of ware at the bottom of outer side wall 5 to ware between inner side-wall 6, and indicator region 13 is the border circular areas at the bottom of ware at the bottom of inner side-wall 6 to ware between diapire 4 center of circle.
Described ware lid 3 in the present embodiment, as shown in Figure 2,4, comprising:
Ware lid diapire 7 is circle, and it is 93mm that diameter is comparatively greater than diapire 4 value at the bottom of ware, and thickness is 1.5mm;
Ware lid outer side wall 8 is ring-type, and diameter equals ware lid diapire 7 diameter, and being highly less than outer side wall 5 value at the bottom of ware is 15mm, is vertically connected at ware lid diapire 7;
Ware lid inner side-wall 9 is ring-type, and diameter is smaller than ware lid diapire 7 diameter, and being highly less than outer side wall 5 value at the bottom of ware is 5mm, is vertically connected at ware lid diapire 7.
Leave annular ditch groove 10 between described ware lid outer side wall 8 and ware lid inner side-wall 9, annular ditch groove 10 width equals outer side wall 5 thickness at the bottom of ware, width value 1.5mm.
Described seal washer 2 is O grommet type seal circle 2, annular ditch groove 10 value in the same size of wire diameter and ware lid outer side wall 8 and ware lid inner side-wall 9 is 1.5mm, internal diameter is annular ditch groove 10 diameter value is 87mm, high temperature resistant, the corrosion resistant materials such as the optional urethane of material, chloroprene rubber, silicon rubber.
This adaptation Novel culture dish can use glass or the transparent plastics material such as PC, transparent ABS to process.
Volatile matter Antibacterial Activity culture dish using method process of the present utility model is as follows:
When using this culture dish, described O grommet type seal circle 2 is closely loaded in the annular ditch groove 10 of ware lid 3, wrap, at 121 DEG C of moist heat sterilization 20 ~ 30min with 1 independent separating at the bottom of ware; In inner side-wall 6 at the bottom of ware, indicator growth sterilising medium is poured in indicator region 13 into, and thickness is about 10 ~ 12mm; After culture medium solidifying, at the bottom of ware, indicator is accurately inoculated in 1 center vaccination 11 position; Test substance region 14 needed amount at the bottom of ware at the bottom of inner side-wall 6 and ware between outer side wall 5 adds volatile compound to be measured or inoculates can produce Identification of Volatile Fungistatic Compounds microorganism, build ware lid 3 and pressing closely; After cultivation after a while, just by the bottom of ware 1 concentric scale ring 12 read indicator data.
More than show and describe ultimate principle of the present utility model, principal character and advantage of the present utility model.The technician of the industry should understand; the utility model is not restricted to the described embodiments; what describe in above-described embodiment and specification sheets just illustrates principle of the present utility model; under the prerequisite not departing from the utility model spirit and scope; the utility model also has various changes and modifications, and these changes and improvements all fall within the scope of claimed the utility model.The claimed scope of the utility model is defined by appending claims and equivalent thereof.
Claims (10)
1. a volatile matter Antibacterial Activity culture dish, is characterized in that, comprises (1) at the bottom of ware, ware lid (3), seal washer (2);
Described seal washer (2) is embedded in ware lid (3) annular ditch groove (10);
At the bottom of described ware, (1) comprising: inner side-wall (6) at the bottom of outer side wall (5), ware at the bottom of diapire at the bottom of ware (4), ware, diapire (4) at the bottom of described ware has measures mark, at the bottom of described ware, outer side wall (5) is greater than inner side-wall at the bottom of ware (6), all be connected to diapire at the bottom of ware (4), (1) at the bottom of ware is divided into two regions.
2. a kind of volatile matter Antibacterial Activity culture dish according to claim 1, it is characterized in that: diapire (4) at the bottom of described ware is circle, its center of circle has annular vaccination (11) to mark.
3. a kind of volatile matter Antibacterial Activity culture dish according to claim 2, is characterized in that: described measurement mark is scale marker.
4. a kind of volatile matter Antibacterial Activity culture dish according to claim 3, is characterized in that: described scale marker, is concentric scale ring (12).
5. a kind of volatile matter Antibacterial Activity culture dish according to claim 4, is characterized in that: described concentric scale ring (12) extends out to inner side-wall at the bottom of ware (6) from diapire (4) center of circle at the bottom of ware.
6. a kind of volatile matter Antibacterial Activity culture dish according to claim 1, is characterized in that: outer side wall (5) at the bottom of described ware is ring-type, and diameter equals diapire at the bottom of ware (4) diameter.
7. a kind of volatile matter Antibacterial Activity culture dish according to claim 6, it is characterized in that: inner side-wall (6) at the bottom of described ware, for ring-type, diameter is less than diapire at the bottom of ware (4) diameter, and height is lower than outer side wall at the bottom of ware (5).
8. a kind of volatile matter Antibacterial Activity culture dish according to claim 7, is characterized in that: (1) at the bottom of described ware is divided into test substance region (14) and indicator region (13).
9. a kind of volatile matter Antibacterial Activity culture dish according to claim 8, it is characterized in that: described test substance region is the annular region at the bottom of ware at the bottom of outer side wall (5) to ware between inner side-wall (6), indicator region is the border circular areas at the bottom of ware at the bottom of inner side-wall (6) to ware between diapire (4) center of circle.
10. a kind of volatile matter Antibacterial Activity culture dish according to claim 1, is characterized in that: described ware lid (3), comprising:
Ware lid diapire (7) is circle, and diameter is comparatively greater than diapire at the bottom of ware (4);
Ware lid outer side wall (8) is ring-type, and diameter equals ware lid diapire (7) diameter, is highly less than outer side wall at the bottom of ware (5), is connected to ware lid diapire (7);
Ware lid inner side-wall (9) is ring-type, and diameter is smaller than ware lid diapire (7) diameter, is highly less than outer side wall at the bottom of ware (5), is connected to ware lid diapire (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520520229.8U CN204874519U (en) | 2015-07-17 | 2015-07-17 | Culture dish is used to volatile materials bacteriostatic activity survey |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520520229.8U CN204874519U (en) | 2015-07-17 | 2015-07-17 | Culture dish is used to volatile materials bacteriostatic activity survey |
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| Publication Number | Publication Date |
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| CN204874519U true CN204874519U (en) | 2015-12-16 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201520520229.8U Expired - Fee Related CN204874519U (en) | 2015-07-17 | 2015-07-17 | Culture dish is used to volatile materials bacteriostatic activity survey |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2708899A1 (en) * | 2018-08-10 | 2019-04-11 | Univ Leon | CULTIVATION CHAMBER FOR MICROBIOLOGICAL COMPETITION TESTING THROUGH VOLATILE COMPOUNDS (Machine-translation by Google Translate, not legally binding) |
-
2015
- 2015-07-17 CN CN201520520229.8U patent/CN204874519U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2708899A1 (en) * | 2018-08-10 | 2019-04-11 | Univ Leon | CULTIVATION CHAMBER FOR MICROBIOLOGICAL COMPETITION TESTING THROUGH VOLATILE COMPOUNDS (Machine-translation by Google Translate, not legally binding) |
| WO2020030835A1 (en) * | 2018-08-10 | 2020-02-13 | Universidad De León | Culture chamber for microbiological competition assays using volatile compounds |
| JP2021531735A (en) * | 2018-08-10 | 2021-11-25 | ウニヴェルシダー デ レオン | Culture chamber for microbial competition assays using volatile compounds |
| JP7357370B2 (en) | 2018-08-10 | 2023-10-06 | ウニヴェルシダー デ レオン | Culture chamber for microbial competition assays using volatile compounds |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20151216 Termination date: 20170717 |