CN203976776U - A kind of microbiological incubator - Google Patents
A kind of microbiological incubator Download PDFInfo
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- CN203976776U CN203976776U CN201420283321.2U CN201420283321U CN203976776U CN 203976776 U CN203976776 U CN 203976776U CN 201420283321 U CN201420283321 U CN 201420283321U CN 203976776 U CN203976776 U CN 203976776U
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- casing
- incubator
- ventage
- incubator according
- described casing
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- 230000002906 microbiologic effect Effects 0.000 title claims abstract description 12
- 229930195733 hydrocarbon Natural products 0.000 claims description 14
- 150000002430 hydrocarbons Chemical class 0.000 claims description 14
- 239000004215 Carbon black (E152) Substances 0.000 claims description 12
- 230000001954 sterilising effect Effects 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 238000000151 deposition Methods 0.000 claims description 2
- 244000005700 microbiome Species 0.000 abstract description 25
- 238000001514 detection method Methods 0.000 abstract description 7
- 241000894006 Bacteria Species 0.000 description 11
- 238000012258 culturing Methods 0.000 description 5
- 230000000813 microbial effect Effects 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000003647 oxidation Effects 0.000 description 3
- 238000007254 oxidation reaction Methods 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000001273 butane Substances 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- IJDNQMDRQITEOD-UHFFFAOYSA-N n-butane Chemical compound CCCC IJDNQMDRQITEOD-UHFFFAOYSA-N 0.000 description 2
- OFBQJSOFQDEBGM-UHFFFAOYSA-N n-pentane Natural products CCCCC OFBQJSOFQDEBGM-UHFFFAOYSA-N 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 206010019909 Hernia Diseases 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 239000006916 nutrient agar Substances 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a kind of microbiological incubator, comprise casing, inflation mechanism and negative pressure device, the first ventage and the second ventage that are communicated with inside and outside casing are set on the wall of casing, and the first ventage is connected with inflation mechanism is sealed, and the second ventage is connected with negative pressure device is sealed.By arranging like this, can guarantee gas concentration required in incubator, for accuracy and the reliability of the dull and stereotyped detection method of oil gas microorganism provides safeguard.
Description
Technical field
The utility model relates to a kind of microbiological incubator, is specially the microbiological incubator of the oil and gas indication bacterium flat band method detection being applicable in oil-gas exploration.
Background technology
Traditional microbial prospecting technology of oil & be utilize that microorganism enrichment is cultivated, quantity that physiology, the biochemical characteristic of separated, counting and microbial metabolites are studied oil and gas indication microorganism in surface soil sample is abnormal, thereby under prediction, cover the distribution situation of hydrocarbon-bearing pool.Flat-plate bacterial colony is cultivated counting process, its principle is through gradient dilution by pedotheque to be measured, microorganism is fully dispersed into individual cells, getting a certain amount of diluent is applied on nutrient agar flat board, after constant temperature culture, by individual cells growth and breeding, form macroscopic bacterium colony, statistics bacterium colony number, according to its extension rate and sampling inoculum size can converse in sample containing bacterium number.In oil gas microorganism culturing separating experiment is analyzed, the microbiological incubator visual plant that is absolutely necessary.
The bacterial classification that oil gas microorganism plating method mainly detects is at present methane-oxidizing bacteria and butane oxidation bacterium, and these two kinds of oil and gas indication bacterium are has a liking for warm type bacterium, and culture temperature is 30 ℃ of left and right, and culture cycle is 7-14 days.Existing microbiological incubator specific aim is not strong, and suitable microorganism growth environment can not be provided, and the in the situation that of longer in the microorganism separation and Culture cycle like this, easily causes microorganism separation and purification effect undesirable, and microorganism detection count results is inaccurate.
Utility model content
For above-mentioned problem, the utility model aims to provide a kind of microbiological incubator, and it can accurately control the hydrocarbon gas concentration in incubator, and suitable microorganism growth environment is provided, and guarantees accuracy and the reliability of detected result.
According to a kind of microbiological incubator of the present utility model, comprise casing, inflation mechanism and negative pressure device, the first ventage and the second ventage that are communicated with inside and outside casing are set on the wall of casing, and the first ventage is connected with inflation mechanism is sealed, and the second ventage is connected with negative pressure device is sealed.By arranging like this, can guarantee gas concentration required in incubator, for accuracy and the reliability of the dull and stereotyped detection method of oil gas microorganism provides safeguard.
In one embodiment, inflation mechanism comprises for depositing the gas-holder of hydrocarbon gas.This gas-holder can provide for the cultivation of oil gas microorganism hydro carbons carbon source, for this reason for utilizing the accuracy of the enumeration of the oil gas microorganism that flat band method cultivates that technical support is provided.
In one embodiment, in the inner chamber of casing, be provided for holding the opening container of sterilized water.By this set, guarantee to there is certain humidity in incubator, prevent the shrink of agar in flat board and dry and cracked.Thereby guarantee reliability and accuracy that follow-up cultivation detects.
Preferably, opening container structure is the tank that is arranged on the diapire center of casing.By this set, guarantee the harmony of humidity in cabinets cavity, be conducive to improve reliability and the accuracy of cultivating detection.And this simple in structure, easily manufactured, be easy to realize.
Further preferably, the volumetric ratio of tank volume and casing is 1:300～1:800.By this set, make the amount of the sterilized water in opening container can meet the needs of oil gas microorganism growth environment, make all there is certain humidity in the inherent whole culture cycle of incubator, guaranteed the more favourable environment of oil gas microorganism growth.
In one embodiment, negative pressure device comprises vacuum pump.It is used for regulating the concentration of air in incubator, discharges entrap bubble and carries out gas displacement.
In one embodiment, also comprise the pressure-detecting device that can detect the gaseous tension in casing.It is for detection of the gaseous tension in incubator.
The dividing plate in the interior space that can adjust casing is set in one embodiment, in the inner chamber of casing.It is used for adapting to the variation of requisite space in casing, optimizes and utilizes the space in casing.
Preferably, dividing plate is the netted dividing plate of stainless steel.It is simple in structure, is easy to realize.
In one embodiment, also comprise the sterilizing unit being arranged in casing.Preferably, sterilizing unit is ultraviolet lamp.Before cultivating, can carry out sterilizing to box house, the growing environment of microorganism cleaning is provided.
Compared with prior art, the utility model has the advantage of, be provided with negative pressure device and inflation mechanism, can effectively control hydrocarbon gas content in incubator, facilitate the supply of hydrocarbon gas and the extraction of residual gas; By setting, hold the tank of sterilized water, guarantee the humidity in incubator, prevent dull and stereotyped dry and cracked and shrink; Utilize ultraviolet lamp sterilizing, the growing environment of microorganism cleaning is provided; And its internal space can freely be adjusted by the dividing plate arranging.These arrange the reliability that facilitates feasibility and experimental result that has all guaranteed experimental implementation, for the detection in microbial prospecting technology of oil & provides guarantee.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, preferred embodiment of the present utility model is described in detail.In the drawings:
Fig. 1 has shown microbiological incubator.
Accompanying drawing is not according to actual scale.
Embodiment
To introduce according to preferred embodiment of the present utility model hereinafter.
Fig. 1 schematically illustrates microbiological incubator 100.As shown in Figure 1, incubator 100 comprises casing 1.The the first inside and outside ventage 6 and the second ventage 7 that are communicated with casing 1 are set on the wall of casing 1.Wherein, the first ventage 6 is connected with inflation mechanism 4 is sealed, and the second ventage 7 is connected with negative pressure device 5 is sealed.Negative pressure device 5 has vacuum pump 9, can extract the part air in casing 1 out, is used for regulating the air concentration in casing 1, and gets rid of entrap bubble and carry out gas displacement.Inflation mechanism 4 has the hydrocarbon gas of storing gas-holder 8, and for example gas-holder 8 can be steel cylinder.Inflation mechanism 4 is for controlling the volume that is filled with casing 1 hydrocarbon gas.Incubator 100 also comprises pressure-detecting device 10, and it can express the gaseous tension in current casing 1.By above-mentioned layout, can effectively control the content of the hydrocarbon gas in casing 1, make the atmosphere surrounding in casing 1 meet culturing micro-organisms demand.
Incubator 100 is interior arranges opening container 11.Preferably, opening container 11 can be the tank of the diapire center that is arranged on casing 1.Therefore this tank 11 is for holding aseptic deionized water, and due to the homo(io)thermism in casing 1, evaporation of water also keeps homogeneity, guaranteed to have constant humidity in casing 1, has avoided the shrink of agar in flat board and dry and cracked.Simultaneously this kind arranges the gnotobasis that can meet in casing 1 and the harmony of humidity.Further preferably, the volumetric ratio of the volume of tank 11 and casing 1 is 1:300～1:800, and still more preferably, the volumetric ratio of the volume of tank 11 and casing 1 is 1:500.By this set, make the amount of the sterilized water in tank 11 can meet the needs of oil gas microorganism growth environment, guarantee at whole microorganism culturing interior more favourable environment all with oil gas microorganism growth of casing 1 in the cycle.
The interior dividing plate 14 that arranges of casing 1.It can be dismantled and energy up-down adjustment position, meets the adjustability of the internal space of casing 1, thus convenient dull and stereotyped putting.Preferably, dividing plate 14 can be the netted dividing plate of stainless steel.For example grooved slideway can be set on the sidewall of casing 1, dividing plate 14 can slip into and skid off along slideway, is convenient to the installation and removal of dividing plate 14.Protruding type can also be directly set on the inwall of casing 1 and support, dividing plate 14 also can be extracted out and push on protruding type supports.Can 2～4 layers of dividing plate 14 be set casing 1 is interior, with the interior optimal use of space to casing 1.Grooved slideway and protruding type support and with the fit structure of dividing plate 14, those skilled in the art are known, therefore not shown in figures.
Incubator 100 also comprises sterilising plant 13.Sterilising plant 13 is arranged on the inwall of casing 1, can open 13 pairs of casing 1 inside of sterilising plant and carry out sterilizing before culturing micro-organisms.Preferably, sterilising plant 13 can be ultraviolet lamp.
Casing 1 inner top of incubator 100 is equipped with illumination part 12, has solved in incubator 100 light darker, the flat board of taking, observes inconvenient problem.Preferably, illumination part 12 can be hernia luminescent lamp.
Incubator 100 also comprises the control panel 3 being arranged on casing 1, and control panel 3 is for controlling the temperature in casing 1, and it is connected with heater element, refrigeration device (not shown), and with for showing that the display screen 2 of the temperature in casing 1 is connected.By the above-mentioned temperature of adjusting in casing 1 that arranges, to meet culturing micro-organisms demand.
This incubator 100 is provided with negative pressure device 5 and inflation mechanism 4, can effectively control hydrocarbon gas content in casing 1, facilitates the supply of hydrocarbon gas and the extraction of residual gas; By aseptic tank 11 being set casing 1 is interior, guarantee the humidity in casing 1, prevent dull and stereotyped dry and cracked and shrink; Utilize ultraviolet lamp 13 sterilizings, the growing environment of microorganism cleaning is provided; Utilize dividing plate 14 to make its internal space capable of regulating.The flat-plate bacterial colony quantity that this incubator 100 is applicable to the hydro carbons indicators such as methane-oxidizing bacteria in Microbial Prospecting of Oil and Gas, butane oxidation bacterium and hydrocarbon oxidation bacterium detects, for the accuracy of cultivation results provides the support in engineering, can be applied to the heavy industrialization test in Microbial Prospecting of Oil and Gas.
The foregoing is only preferred implementation of the present utility model; but the utility model protection domain is not limited to this; any those skilled in the art is in the disclosed technical scope of the utility model; can easily change or change, and this change or change and all should be encompassed in protection domain of the present utility model within.Therefore, protection domain of the present utility model should be as the criterion with the protection domain of claims.
Claims (10)
1. a microbiological incubator, it is characterized in that, comprise casing, inflation mechanism and negative pressure device, the first ventage and the second ventage that are communicated with inside and outside described casing are set on the wall of described casing, described the first ventage is connected with described inflation mechanism is sealed, and described the second ventage is connected with described negative pressure device is sealed.
2. incubator according to claim 1, is characterized in that, described inflation mechanism comprises for depositing the gas-holder of hydrocarbon gas.
3. incubator according to claim 1, is characterized in that, is provided for holding the opening container of sterilized water in the inner chamber of described casing.
4. incubator according to claim 3, is characterized in that, described opening container structure is the tank that is arranged on the diapire center of described casing.
5. incubator according to claim 4, is characterized in that, the volumetric ratio of described tank volume and described casing is 1:300～1:800.
6. incubator according to claim 2, is characterized in that, described negative pressure device comprises vacuum pump.
7. incubator according to claim 6, is characterized in that, also comprises the pressure-detecting device that can detect the gaseous tension in described casing.
8. incubator according to claim 1, is characterized in that, the dividing plate that can adjust the interior space of described casing is set in the inner chamber of described casing.
9. incubator according to claim 8, is characterized in that, described dividing plate is the netted dividing plate of stainless steel.
10. incubator according to claim 1, is characterized in that, also comprises the sterilizing unit being arranged in described casing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420283321.2U CN203976776U (en) | 2014-05-29 | 2014-05-29 | A kind of microbiological incubator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420283321.2U CN203976776U (en) | 2014-05-29 | 2014-05-29 | A kind of microbiological incubator |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203976776U true CN203976776U (en) | 2014-12-03 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420283321.2U Expired - Lifetime CN203976776U (en) | 2014-05-29 | 2014-05-29 | A kind of microbiological incubator |
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| Country | Link |
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| CN (1) | CN203976776U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107229081A (en) * | 2016-03-24 | 2017-10-03 | 中国石油化工股份有限公司 | Analogue means for oil gas microbe research |
| CN108359593A (en) * | 2017-01-26 | 2018-08-03 | 中国石油化工股份有限公司 | A kind of oil-gas reservoir microcosm model and the method for studying oil and gas indication microorganism |
-
2014
- 2014-05-29 CN CN201420283321.2U patent/CN203976776U/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107229081A (en) * | 2016-03-24 | 2017-10-03 | 中国石油化工股份有限公司 | Analogue means for oil gas microbe research |
| CN108359593A (en) * | 2017-01-26 | 2018-08-03 | 中国石油化工股份有限公司 | A kind of oil-gas reservoir microcosm model and the method for studying oil and gas indication microorganism |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |