CN107229081A - Analogue means for oil gas microbe research - Google Patents
Analogue means for oil gas microbe research Download PDFInfo
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- CN107229081A CN107229081A CN201610172866.XA CN201610172866A CN107229081A CN 107229081 A CN107229081 A CN 107229081A CN 201610172866 A CN201610172866 A CN 201610172866A CN 107229081 A CN107229081 A CN 107229081A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01V—GEOPHYSICS; GRAVITATIONAL MEASUREMENTS; DETECTING MASSES OR OBJECTS; TAGS
- G01V9/00—Prospecting or detecting by methods not provided for in groups G01V1/00 - G01V8/00
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Abstract
The present invention proposes a kind of analogue means for oil gas microbe research.The analogue means includes container；The screen assembly in the inner chamber of container is arranged on, the inner chamber of container is divided into upper space and lower space by screen assembly；It is arranged on the closure at the opening of container；It is arranged on the air intake assembly on the side wall being located at lower space of container；It is arranged on closure and takes pneumatic module；It is arranged on the vacuum elements on closure.It can simulate the environment under oil-gas reservoir lighter hydrocarbons seepage to carry out Microbial Prospecting of Oil and Gas experiment by the analogue means.
Description
Technical field
The present invention relates to microbial prospecting technology of oil ＆ field, and in particular to a kind of for oil gas microbe research
Analogue means.
Background technology
One of microbial prospecting technology of oil ＆ important foundation is that oil gas vertical migration is theoretical, mainly studies near surface
The abnormal dependency relation with underground deep oil-gas reservoir of Soil Microorganism.Carry out using microbial prospecting technology of oil ＆
Prediction of oil-gas reserve, determines architectonic oil-gas possibility and petroleum distribution, indicates oil-gas reservoir position, for improving
Oil-gas exploration success rate is significant.
A variety of oil and gas indication bacterium cultures and molecular biosciences are had been set up currently for microbial prospecting technology of oil ＆
Detection method is learned, and can meet the high-throughout detection of mass and is required, it is existing in the wild to these detection methods
Field has also carried out more careful assessment.The foundation of microbial prospecting technology of oil ＆ is a forward modeling research process,
Namely pass through micro organism quantity in pedotheque above oil-gas reservoir and oil gas background area pedotheque and species
Research, has the obligate microorganism for being capable of the distribution of Indication of Oil-Gas Tibetan to obtain.And Microbial Prospecting of Oil and Gas is final
Target is by detecting this obligate micro organism quantity and population in research work area surface soil, favorably being surveyed to predict
Exploratory area, is a kind of inverting Research Thinking.Therefore, Microbial Prospecting of Oil and Gas oil prospecting technology be built upon it is perfect just
Drill on theoretical foundation, but during forward modeling theory is set up, researcher is for field soil sample oil
The understanding of gas background information also has some limitations, i.e., absolutely whether can not affirm a pedotheque
Come from directly over oil-gas reservoir or above background area, it is impossible to determine whether microbiology turbidity is by hydro carbons in sample
Caused by Micro blazed-grating.In addition, the change mechanism of existing correlation technique only to hydro carbons under hydrocarbon microseepage in soil
Research is simulated, is not directed to contact the research method of close soil oil gas microorganism with oil gas, therefore
The problems of Microbial Prospecting of Oil and Gas field is not can effectively solve the problem that.
In view of problem above, it would be desirable to be created that the simulated environment under oil-gas reservoir lighter hydrocarbons seepage, and soil-like
Product are placed in the artificial microcosm simulated environment of this determination oil gas background information, by the culture of some cycles,
To analyze the changing rule of oil and gas indication microorganism in pedotheque, its quantity and population change mechanism are investigated, is obtained
Take the sensitive indicator microoraganism of oil gas.More direct experimental basis will be provided for Microbial Prospecting of Oil and Gas theory,
Laid the foundation for the abnormal synthesis identifying technique of oil gas microbiologic population.
Thus, it would be desirable to design a kind of analogue means for oil gas microbe research light to simulate oil-gas reservoir
Environment under hydrocarbon seepage is to carry out theoretical research.
The content of the invention
For some or all of of the above-mentioned technical problem in the presence of prior art, the present invention proposes one kind
Analogue means for oil gas microbe research.Using the analogue means, subterranean oil gas reservoir Micro blazed-grating can be simulated
Hydrocarbon environment, the growing environment for determining oil gas background information is provided for edaphon.
According to the invention it is proposed that a kind of analogue means for oil gas microbe research, including：
Container,
The screen assembly in the inner chamber of container is arranged on, the inner chamber of container is divided into upper space and lower sky by screen assembly
Between,
The closure at the opening of container is arranged on,
The air intake assembly on the side wall being located at lower space of container is arranged on,
It is arranged on closure and takes pneumatic module,
It is arranged on the vacuum elements on closure.
In one embodiment, screen assembly is detachable is arranged on the inwall of container, and screen assembly bag
Include the filter of multiple top-and-bottom spaced settings, each mesh area of underlying filter be less than it is above every
Each mesh area of net.
In one embodiment, the mesh structure of filter is rectangle, and point of the quadratic mesh of adjacent filter
Cloth direction intersects.
In one embodiment, can detachable setting supporting leg on screen assembly.
In one embodiment, air intake assembly includes：
It is arranged on and is provided with what can be connected with the lower space on the flange on the outer wall of the container, the flange
First air inlet,
The first seal for blocking first air inlet at first air inlet is arranged on, and
For the first pressure cap being pressed on the first seal on the flange, set on first pressure cap
It is equipped with the second air inlet corresponding with first air inlet.
In one embodiment, the second seal between flange and the outer wall of container.
In one embodiment, the screen assembly is located on 1/3rd of the container height, and/or institute
Air intake assembly is stated under 1/2nd of the container height.
In one embodiment, pneumatic module is taken to include：
What is be fixedly connected with closure takes gas seat, and gas is taken taking be provided with gas seat inside and outside connection container first
Mouthful,
The 3rd seal for taking and being used to block the first air hatch on gas seat is arranged on,
For the 3rd seal to be pressed on to the second pressure cap taken on gas seat, having on the second pressure cap can be with first
The second corresponding air hatch of air hatch.
In one embodiment, vacuum elements include：
The valve seat being fixedly connected with closure, has the bleeding point inside and outside connection container on valve seat,
It is arranged on the bleed-off passage that is provided with and can selectively be connected with bleeding point on the needle at bleeding point, needle.
In one embodiment, valve seat is threadedly coupled with needle.
Compared with prior art, it is an advantage of the current invention that pedotheque can be put into container, and pass through
Air intake assembly is filled with hydrocarbon gas into container, to simulate Micro blazed-grating hydrocarbon environment.It can then study in container
In soil oil and gas indication microbiologic population feature and number change mechanism, be that Microbial Prospecting of Oil and Gas theory is carried
For more direct experimental basis, also laid the foundation for the abnormal synthesis identifying technique of oil gas microbiologic population.
Brief description of the drawings
The preferred embodiments of the present invention are described in detail below in conjunction with accompanying drawing, in figure：
Fig. 1 is shown is used for the analogue means of oil gas microbe research according to an embodiment of the invention；
Fig. 2 shows the profilograph of analogue means according to an embodiment of the invention；
Fig. 3 shows the profile at the A-A from Fig. 2；
In the accompanying drawings, identical part uses identical reference, and accompanying drawing is not drawn according to actual ratio.
Embodiment
Below in conjunction with accompanying drawing, the present invention will be further described.
Fig. 1 is shown is used for the analogue means 100 of oil gas microbe research according to an embodiment of the invention.Such as
Shown in Fig. 1, analogue means 100 includes container 1, screen assembly 2 (as shown in Figure 2), closure 3, entered
Pneumatic module 4, take pneumatic module 5 and vacuum elements 6.Wherein, screen assembly 2 is arranged in the inner chamber of container 1,
So that the inner chamber of container 1 is divided into upper space 11 and lower space 12, it can find out in Fig. 2.Closure 3 is covered
At the opening of container 1, for the inner chamber of sealing container 1.Air intake assembly 4 is arranged on the wall of container 1,
And at lower space 12, for injection gas in downward space 12.Pneumatic module 5 is taken to be arranged on closure
On 3, for by taking pneumatic module 5 to take gas by container 1.Vacuum elements 6 are arranged on closure 3, with
For replacing the residual gas in container 1 by vacuum elements 6.
When being tested, first pedotheque can be placed on screen assembly 2, and form closed in container 1
Environment.Then, by being filled with hydrocarbon gas in the downward space 12 of air intake assembly 4, (for example hydrocarbon gas can
Think methane, ethane, propane, butane mixture gase), to simulate the hydrocarbon environment of subterranean oil gas reservoir Micro blazed-grating,
The growing environment for determining oil gas background information is provided for edaphon.In pedotheque incubation, it can lead to
The hydrocarbon gas crossed in the inner chamber for taking pneumatic module 5 to obtain container 1, it is possible to chromatogram detection is carried out, to monitor appearance
The component and concentration of hydrocarbon gas in device 1.During soil incubation, it can also be put by vacuum elements 6
The residual gas changed in the inner chamber of container 1.Thus, it can simulate that hydro carbons is micro- to be oozed by using the analogue means 100
Oil gas micro organism quantity and group's change mechanism in environment are leaked, theoretical foundation is provided for Microbial Prospecting of Oil and Gas.
According to the present invention, it is arranged on as shown in Fig. 2 screen assembly 2 is detachable on the inwall of container 1, and
And screen assembly 2 includes the filter 21 of multiple top-and-bottom spaced settings.Each mesh of underlying filter 21
Area is less than each mesh area of filter 21 above.Before pedotheque is put into container 1,
Need to remove impurity, and handled through screen cloth homogenization.Then, treated pedotheque will be uniformed and is put into position
In on the filter 21 of the top.Now, pedotheque is possible to downwards drop by the filter 21 of the top,
And due to lower section filter 21 each mesh area be less than in top filter 21 each mesh area, then under
The filter 21 of side prevents further dropping for pedotheque.So as to avoid pedotheque by above-mentioned setting
Excessive drops on the bottom wall of container 1, it is ensured that the cleaning of the inner chamber of container 1, while ensure that preferably
Test effect.
Preferably, filter 21 can be made up of plastic or other material, to avoid filter 21 and other material (such as water
Or hydrocarbon gas etc.) occur chemical reaction and influence result of the test.Also, for easily manufactured, filter 21
On each mesh can be the hole being evenly distributed on filter 21.And the hole on filter 21 can for it is circular, square,
The arbitrary shape such as polygon, quincunx.But, in order to reduce processing cost, it is further preferred that filter 21
Mesh structure be rectangle.Filter 21 with this structure is easy to process, convenient manufacture.For example, adjacent
Two filters 21 quadratic mesh distribution arrangement intersect.It is, the rectangle net of two filters 21 up and down
The distribution arrangement in hole can not be overlapping, is dropped area with reducing the soil of screen assembly 2.Thus, set by above-mentioned
Put, in the case where can guarantee that gas can pass through screen assembly 2, it is to avoid more soil drop to container
On 1 bottom wall.
In one embodiment, as shown in Fig. 2 stacker 13 can be set on the inwall of container 1, to pacify
Put screen assembly 2.On direction from top to bottom, the internal diameter of container 1 can reduce, to form holding for boss-shaped
Joint chair 13, for preventing screen assembly 2.Container is arranged on by what this set screen assembly 2 can be stablized
In 1 inner chamber, and rapid positioning can be realized.
In another embodiment, supporting leg (not shown) can also be set energy detachable on screen assembly 2.
Screen assembly 2 can be arranged in the inner chamber of container 1 by supporting leg.Simultaneously as supporting leg can be torn open from filter 21
Unload down, thus, in use, the length of supporting leg can be adjusted by changing different supporting legs, with
Screen assembly 2 is placed in the diverse location of container 1, so as to meet different test requirements documents.
As shown in figure 3, air intake assembly 4 includes flange 41, the pressure cap 43 of first seal 42 and first.Wherein,
Flange 41 is arranged on the outer wall of container 1, and can be connected with lower space 12 first is provided with flange 41
Air inlet 44.First seal 42 is arranged at the first air inlet 44, for the first air inlet of sealed closure
Mouth 44.Preferably, first seal 42 can be rubber stopper.First pressure cap 43 is fixedly connected with flange 41,
So that first seal 42 is pressed on flange 41, to ensure the sealing effectiveness at air intake assembly 4.Preferably,
First pressure cap 43 is threadedly coupled with flange 41.Meanwhile, it is provided with the first pressure cap 43 and the first air inlet
44 corresponding second air inlets 45.Preferably, the second seal 46 between the outer wall of flange 41 and container 1,
To ensure the sealing between flange 41 and the outer wall of container 1.Preferably, second seal 46 is that rubber is close
Seal.In process of the test, using syringe by piercing through first seal 42 after the second air inlet 45, with
Gas is injected into container 1.If necessary to replace during the gas in container 1, the first pressure cap can also be dismantled down
43 to cause lower space 12 to be in communication with the outside by the first air inlet 44.
In order to be able to preferably simulate the hydrocarbon environment of subterranean oil gas reservoir Micro blazed-grating, while in order to ensure in container 1
The Optimum utilization of chamber, screen assembly 2 is located on 1/3rd of the height of container 1.And/or air intake assembly 4
Under 1/2nd of the height of container 1.For example, screen assembly 2 may be at the height interposition of container 1
Put place.And air intake assembly 4 is located at one of four parts of the height of container 1 position.
As shown in Fig. 2 taking pneumatic module 5 to include taking gas the 51, the 3rd seal 52 of seat and the second pressure cap 53.Take
Gas seat 51 is fixedly connected with closure 3.Also, it is provided with taking on gas seat 51 inside and outside connection container 1
First air hatch 54.3rd seal 52, which is arranged on, to be taken on gas seat 51 for the first air hatch 54 of closure.
Second pressure cap 53 is taken on gas seat 51 with taking gas seat 51 to be fixedly connected for the 3rd seal 52 is pressed on.
Preferably, the second pressure cap 53 is with taking gas seat 51 to be threadedly coupled.Meanwhile, having on the second pressure cap 53 can be with
The second corresponding air hatch 55 of first air hatch 54.Preferably, the 3rd seal 52 is rubber stopper.In examination
During testing, using syringe by piercing through the 3rd seal 52 after the second air hatch 55, taken out of container 1
Go out gas, then chromatogram detection etc. is carried out to gas, with the gas component and concentration in prosecution container 1.
As shown in Fig. 2 vacuum elements 6 include valve seat 61 and needle 62.Wherein valve seat 61 is fixedly installed on close
In capping 3, and there is the bleeding point 63 connected inside and outside container 1 on valve seat 61.Needle 62 is arranged on
The bleed-off passage 64 that can be connected with the selectivity of bleeding point 63 is provided with bleeding point 63, and on needle 62.
Preferably, needle 62 is threadedly attached on valve seat 61, and activity is unscrewed relative to valve seat 61 in needle 62
When, bleeding point 63 is connected with bleed-off passage 64.Now, by first seal 42 by the first air inlet 44
Remove, to connect the inner chamber and the external world of container 1, and the equipment such as vavuum pump are connected on needle 62, will held
Gas displacement in device 1 comes out.And after needle 62 is screwed relative to valve seat 61, bleeding point 63 and pumping
Passage 64 ends, with sealing container 1, and ensures the sealing of the inner chamber of container 1.
In order to easy to operate, simplify the structure of analogue means 100, as shown in Fig. 2 closure 3 can pass through spiral shell
Line type of attachment is arranged at the opening of container 1.And set the 4th to seal between closure 3 and container 1
Part 7, to ensure the sealing of container 1, preferably simulates the hydrocarbon environment of subterranean oil gas reservoir Micro blazed-grating.
The preferred embodiment of the present invention is the foregoing is only, but the scope of the present invention is not limited thereto, and is appointed
What those skilled in the art easily can be changed or change in technical scope disclosed by the invention, and
This be altered or varied should all be included within the scope of the present invention.Therefore, protection scope of the present invention should
It is defined by the protection domain of claims.
Claims (10)
1. a kind of analogue means for oil gas microbe research, it is characterised in that including：
Container,
The screen assembly in the inner chamber of the container is arranged on, the inner chamber of the container is divided into by the screen assembly
Upper space and lower space,
The closure at the opening of the container is arranged on,
The air intake assembly on the side wall being located at the lower space of the container is arranged on,
It is arranged on the closure and takes pneumatic module,
It is arranged on the vacuum elements on the closure.
2. analogue means according to claim 1, it is characterised in that the screen assembly is detachable
Set on the inner walls of the container, and the screen assembly includes the filter of multiple top-and-bottom spaced settings,
Each mesh area of the underlying filter is less than each mesh area of the filter above.
3. analogue means according to claim 2, it is characterised in that the mesh structure of the filter is
Rectangle, and the distribution arrangement of the quadratic mesh of the adjacent filter intersects.
4. the analogue means according to any one of Claim 1-3, it is characterised in that it is described every
Can detachable setting supporting leg on screen component.
5. the analogue means according to any one of Claim 1-3, it is characterised in that the air inlet
Component includes：
It is arranged on and is provided with what can be connected with the lower space on the flange on the outer wall of the container, the flange
First air inlet,
The first seal for blocking first air inlet at first air inlet is arranged on, and
For the first pressure cap being pressed on the first seal on the flange, set on first pressure cap
It is equipped with the second air inlet corresponding with first air inlet.
6. analogue means according to claim 5, it is characterised in that in the flange and the container
Outer wall between second seal.
7. the analogue means according to any one of Claim 1-3, it is characterised in that the filter
Component is located on 1/3rd of the container height, and/or the air intake assembly is located at the container height
Under 1/2nd.
8. the analogue means according to any one of Claim 1-3, it is characterised in that described to take gas
Component includes：
What is be fixedly connected with the closure takes gas seat, is provided with described take on gas seat in the connection container
The first outer air hatch,
The 3rd seal for taking and being used to block first air hatch on gas seat is arranged on,
For the 3rd seal to be pressed on into second pressure cap taken on gas seat, on second pressure cap
With the second air hatch that can be corresponding with first air hatch.
9. the analogue means according to any one of Claim 1-3, it is characterised in that the pumping
Component includes：
The valve seat being fixedly connected with the closure, has the pumping connected inside and outside the container on the valve seat
Mouthful,
It is arranged on the needle at the bleeding point, the needle and is provided with and can selectively be connected with the bleeding point
Bleed-off passage.
10. analogue means according to claim 9, it is characterised in that the valve seat and the needle spiral shell
Line is connected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610172866.XA CN107229081B (en) | 2016-03-24 | 2016-03-24 | Simulator for oil gas microbe research |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610172866.XA CN107229081B (en) | 2016-03-24 | 2016-03-24 | Simulator for oil gas microbe research |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107229081A true CN107229081A (en) | 2017-10-03 |
| CN107229081B CN107229081B (en) | 2019-05-07 |
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ID=59931882
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610172866.XA Active CN107229081B (en) | 2016-03-24 | 2016-03-24 | Simulator for oil gas microbe research |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107229081B (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203976776U (en) * | 2014-05-29 | 2014-12-03 | 中国石油化工股份有限公司 | A kind of microbiological incubator |
-
2016
- 2016-03-24 CN CN201610172866.XA patent/CN107229081B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203976776U (en) * | 2014-05-29 | 2014-12-03 | 中国石油化工股份有限公司 | A kind of microbiological incubator |
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| Publication number | Publication date |
|---|---|
| CN107229081B (en) | 2019-05-07 |
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