CN109459341A - A kind of experimental provision and method of Organic biological gas output - Google Patents
A kind of experimental provision and method of Organic biological gas output Download PDFInfo
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- CN109459341A CN109459341A CN201811587797.4A CN201811587797A CN109459341A CN 109459341 A CN109459341 A CN 109459341A CN 201811587797 A CN201811587797 A CN 201811587797A CN 109459341 A CN109459341 A CN 109459341A
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- 238000000034 method Methods 0.000 title abstract description 18
- 239000007789 gas Substances 0.000 claims abstract description 158
- 239000011261 inert gas Substances 0.000 claims abstract description 51
- 238000006243 chemical reaction Methods 0.000 claims abstract description 43
- 230000000813 microbial effect Effects 0.000 claims abstract description 38
- 238000003860 storage Methods 0.000 claims abstract description 35
- 239000007788 liquid Substances 0.000 claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 230000001954 sterilising effect Effects 0.000 claims abstract description 11
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 11
- 238000001514 detection method Methods 0.000 claims abstract description 9
- 238000009434 installation Methods 0.000 claims abstract description 8
- 239000007858 starting material Substances 0.000 claims abstract description 6
- 238000007789 sealing Methods 0.000 claims description 41
- 239000003638 chemical reducing agent Substances 0.000 claims description 28
- 239000012530 fluid Substances 0.000 claims description 22
- 239000005416 organic matter Substances 0.000 claims description 12
- 244000005700 microbiome Species 0.000 claims description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 8
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims description 6
- 229910052757 nitrogen Inorganic materials 0.000 claims description 4
- 238000010998 test method Methods 0.000 claims description 4
- 230000007717 exclusion Effects 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims description 3
- 239000003245 coal Substances 0.000 abstract description 14
- 230000015556 catabolic process Effects 0.000 abstract description 6
- 238000006731 degradation reaction Methods 0.000 abstract description 6
- 230000006837 decompression Effects 0.000 abstract description 4
- 230000015572 biosynthetic process Effects 0.000 abstract description 3
- 238000011161 development Methods 0.000 abstract description 3
- 230000004044 response Effects 0.000 abstract description 3
- 239000000203 mixture Substances 0.000 abstract description 2
- 238000002474 experimental method Methods 0.000 description 27
- 239000000243 solution Substances 0.000 description 15
- 230000008859 change Effects 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 8
- 239000001963 growth medium Substances 0.000 description 6
- 238000009629 microbiological culture Methods 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 238000004140 cleaning Methods 0.000 description 4
- 239000002817 coal dust Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000010419 fine particle Substances 0.000 description 4
- 238000002309 gasification Methods 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- 230000001276 controlling effect Effects 0.000 description 3
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- 238000005086 pumping Methods 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000004088 simulation Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000001351 cycling effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- ZZUFCTLCJUWOSV-UHFFFAOYSA-N furosemide Chemical compound C1=C(Cl)C(S(=O)(=O)N)=CC(C(O)=O)=C1NCC1=CC=CO1 ZZUFCTLCJUWOSV-UHFFFAOYSA-N 0.000 description 1
- 239000001307 helium Substances 0.000 description 1
- 229910052734 helium Inorganic materials 0.000 description 1
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N7/00—Analysing materials by measuring the pressure or volume of a gas or vapour
- G01N7/14—Analysing materials by measuring the pressure or volume of a gas or vapour by allowing the material to emit a gas or vapour, e.g. water vapour, and measuring a pressure or volume difference
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/22—Fuels; Explosives
- G01N33/222—Solid fuels, e.g. coal
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/22—Fuels; Explosives
- G01N33/225—Gaseous fuels, e.g. natural gas
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/24—Earth materials
- G01N33/241—Earth materials for hydrocarbon content
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
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- Health & Medical Sciences (AREA)
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
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- Pathology (AREA)
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- Chemical Kinetics & Catalysis (AREA)
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- Oil, Petroleum & Natural Gas (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to coal bed gas, shale gas development technique field, more particularly to the experimental provision and method of a kind of Organic biological gas output.The experimental provision that the present invention passes through temperature-controlling system, microbial reaction system, the gas storage circulatory system and pressurization and a kind of Organic biological gas output of exhaust system composition, by utensil sterilization treatment, coalignment, device installation, starter, six steps of gas and liquid and detection gas and liquid are collected, temperature, pressure and the hydrodynamic condition when microbial degradation of simulated formation original position；Response of stratum temperature is provided by constant temperature water bath system；Different strata pressures is simulated by the decompression valve regulation of high-pressure inert gas；Underground difference flow rate is simulated by constant-flux pump flow control.Biological respinse tank body can straighten the core sample that diameter is 42cm, can also place bulk sample or powdered samples, the sample scope of application is wider.Operation of the present invention method is simple, and economic cost is low, and analog result is closer to stratum actual conditions.
Description
Technical field
The present invention relates to coal bed gas, shale gas development technique field, more particularly to a kind of Organic biological gas output
Experimental provision and method.
Background technique
In coal bed gas, shale gas development field, microorganism gasification technology has the characteristics that volume increase, anatonosis, recycling, because
And there is unique advantage in terms of abandoned mine, low rank coal and shale gas exploitation.Traditional view thinks pre- in abandoned mine
Coal column and the low low coal rank coal seam of coal bed gas content is stayed not to have extraction value, it can be by coal or page using microorganism gasification technology
Petrosa divides organic matter transformation to be biogas, improves reservoir gas-bearing saturation degree；Microbial degradation coal petrography, shale organic matter also have increasing
Add the effect of reservoir permeability.
Microorganism gasification technology has obtained in terms of improving oil recovery factor, thickened oil recovery and city biochemistry
Major progress, but the laboratory research stage is also predominantly stayed in terms of the bioconversion of coal and shale organic matter.Currently, biological
Gas output experimental provision is broadly divided into two kinds, one is being stood in anaerobism bottle or shake culture produces gas, this method is easy to operate,
Cost is relatively low, can cultivate multiple samples simultaneously, and the main stream approach of experiment indoor simulation biogas output at present；One is hairs
Stir culture in fermentation tank, can place bulk or a large amount of organic matter samples, but this method higher cost, be mainly used for engineering experiment
Research, while there is a common problem in both methods, i.e., it is same that organic matter, which consolidates sample and the contact relation of culture solution liquid sample,
When it is static or simultaneously dynamic the problem of, can not really react underground coal and shale organic matter is stationary, and culture solution carries
Microorganism flows through the geological conditions of organic matter.
Summary of the invention
The object of the present invention is to provide a kind of simulation prime stratum temperature, pressure condition, can provide different flow rate items
Part can simulate under coal seam in-situ different temperatures, pressure and flow rate geological conditions, and microbial degradation coal petrography, shale etc. have
Machine matter hydro carbon-generation process and hydrocarbon potentiality, to deepen the experimental provision and method of its output mechanism.
To achieve the above object, the technical solution adopted by the present invention is that:
A kind of experimental provision of Organic biological gas output, including temperature-controlling system, microbial reaction system, gas storage cyclic system
System, pressurization and exhaust system, the first connecting tube, the second connecting tube and the 4th connecting tube；The gas storage circulatory system is at least wrapped
A head space container is included, one end of the microbial reaction system enters top through head space container upper surface by the 4th connecting tube
In empty container, the other end of the microbial reaction system is connect by the second connecting tube with the bottom of the gas storage circulatory system；
The pressurization and exhaust system is connected in the second connecting tube by the first connecting tube；The microbial reaction system and storage
The gas circulatory system is placed in temperature-controlling system.
The temperature-controlling system is using water-bath temperature-controlling system.
The constant water bath box that the water-bath temperature-controlling system uses.
The microbial reaction system includes at least biological respinse tank body, and one end of biological respinse tank body is close by second
Seal washer is removably connected with the second sealing cover, and the top of the second sealing cover passes through the second through-hole and third conduit being provided with
The other end of one end connection, third conduit is connect by filter with the 4th connecting tube；The lower part of second sealing cover is provided with drain
Mouthful；The other end of biological respinse tank body is removably connected with the first sealing cover by the first seal washer, the first sealing cover
Top passes through the first through hole being provided with and connect with one end of the second connecting conduit, and the other end of the second connecting conduit passes through constant-flux pump
It is connect with head space container bottom.
The second two-port valve is connected on the outside of the leakage fluid dram.
The gas storage circulatory system further includes pressure gauge, the 5th connecting conduit, the 4th two-port valve and third two-port valve；Institute
The pressure gauge stated is connected to the upper surface of head space container by third two-port valve, and one end of the 5th connecting conduit passes through top
Empty container bottom surface simultaneously extends in head space container, and the other end of the 5th connecting conduit is connect with the second connecting conduit；Head space holds
The upper surface of device is also provided with air hatch and exhaust outlet, and the 4th two-port valve is connected on exhaust outlet.
The pressurization and exhaust system include high-pressure inert gas, inert gas bottle valve, gas pressure reducer and the
One two-port valve；The input terminal of the high-pressure inert gas and gas pressure reducer connects, the output end of gas pressure reducer and the
The connection of one connecting conduit, the first two-port valve are connected in the first connecting conduit.
Gas in the high-pressure inert gas is high pure nitrogen.
The constant water bath box that the water-bath temperature-controlling system uses, it is anti-that the microbial reaction system includes at least biology
Tank body is answered, one end of biological respinse tank body is removably connected with the second sealing cover, the second sealing cover by the second seal washer
Top pass through the second through-hole for being provided with and connect with one end of third conduit, the other end of third conduit passes through filter and the 4th
Connecting tube connection；The lower part of second sealing cover is provided with leakage fluid dram；The other end of biological respinse tank body can by the first seal washer
Disassembly is connected with the first sealing cover, and the top of the first sealing cover passes through one end of the first through hole and the second connecting conduit that are provided with
The other end of connection, the second connecting conduit is connect by constant-flux pump with head space container bottom；It is connected on the outside of the leakage fluid dram
Second two-port valve；The gas storage circulatory system further includes pressure gauge, the 5th connecting conduit, the 4th two-port valve and third two-way
Valve；The pressure gauge is connected to the upper surface of head space container, one end of the 5th connecting conduit by third two-port valve
It across head space container bottom surface and extends in head space container, the other end of the 5th connecting conduit is connect with the second connecting conduit；
The upper surface of head space container is also provided with air hatch and exhaust outlet, and the 4th two-port valve is connected on exhaust outlet；The pressurization and row
Gas system includes high-pressure inert gas, inert gas bottle valve, gas pressure reducer and the first two-port valve；The high pressure inert
The connection of the input terminal of gas bottle and gas pressure reducer, the output end of gas pressure reducer are connect with the first connecting conduit, the first two-way
Valve is connected in the first connecting conduit.
A kind of test method of the experimental provision of Organic biological gas output, includes the following steps
Step 1: utensil sterilization treatment
By biological respinse tank body, head space container, culture solution, the first sealing cover, the second sealing cover, filter, the first connection
Conduit, the second connecting conduit, third connecting conduit, the 4th connecting conduit, the 5th connecting conduit, the first two-port valve, the second two-way
Valve, third two-port valve, the 4th two-port valve carry out sterilization treatment；
Step 2: coalignment
Organic matter sample is put into through in the processed biological respinse tank body of step 1, culture solution is put at through step 1
In the head space container managed；
Step 3: device installation
All components handled well by step 1 and step 2 are attached；
Step 4: starter
Successively open pressurization and inert gas bottle valve, the gas pressure reducer, the first two-port valve, the four or two in exhaust system
Port valve, continuous high purity inert gas 10-20 minutes logical, surplus air in exclusion microbial reaction system, the gas storage circulatory system closes
The 4th two-port valve is closed, it is gentle to turn off the first two-port valve, inert gas bottle valve to strata pressure is set for regulating gas pressure reducing valve
Then body pressure reducing valve opens temperature-controlling system and advection pumping source switch, recycles culture solution in head space container in the direction of the clock
Flowing；
Step 5: gas and liquid are collected
In different time sections, head gas in head space container is collected from air hatch；Out of leakage fluid dram acquisition bioreactor
Liquid；
6th step, detection gas and liquid
The gas that step 5 is collected carries out gas component and methane concentration detection, and liquid carries out microbial diversity point
Analysis.
The utility model has the advantages that
The present invention passes through temperature-controlling system, microbial reaction system, the gas storage circulatory system and pressurization and exhaust system composition
A kind of experimental provision of Organic biological gas output passes through utensil sterilization treatment, coalignment, device installation, starter, receipts
Collect gas and liquid and detection gas and six steps of liquid, make temperature needed for can accurately controlling reaction in experimentation,
Facilitate the laboratory apparatus cleaning of Preparatory work of experiment stage and ending phase；Biological respinse tank body connects a filter, can prevent
Only the fine particles such as coal dust enter head space container；Pressurization uses one bottle of external high-pressure inert gas, decompression with exhaust system
Initial pressure and anaerobic environment in valve and the first two-port valve autonomous control system, the inert gas pressure in the bottle can be at any time when too low
Topping up or replacement inert gas bottle, it is easy to operate, it is at low cost, save experiment consumptive material.The gas storage circulatory system can store
The gas of generation；It, can be with initial pressure in display system and reality added with pressure gauge on the head space container in the gas storage circulatory system
Middle pressure change is tested, reflects gas generating amount；Constant-flux pump is connected below head space container, provides circulation power for fluid in system.
Temperature, pressure and hydrodynamic condition when simulated formation original position microbial degradation of the present invention；Pass through constant temperature water bath system
System provides response of stratum temperature；Different strata pressures is simulated by the decompression valve regulation of high-pressure inert gas；Pass through advection
Pump discharge controls to simulate underground difference flow rate.
Biological respinse tank body can straighten the core sample that diameter is 42cm, can also place bulk sample or powdered samples,
The sample scope of application is wider.
Equipment operation is simple, and economic cost is low, and analog result is closer to stratum actual conditions.
The above description is only an overview of the technical scheme of the present invention, in order to better understand technology hand of the invention
Section, and can be implemented in accordance with the contents of the specification, and with presently preferred embodiments of the present invention and attached drawing be cooperated to be described in detail such as below
Afterwards.
Detailed description of the invention
It in order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, below will be to institute in embodiment
Attached drawing to be used is needed to be briefly described, it should be apparent that, the accompanying drawings in the following description is only some implementations of the invention
Example, for those of ordinary skill in the art, without creative efforts, can also obtain according to these attached drawings
Obtain other attached drawings.
Fig. 1 is schematic structural view of the invention；
Fig. 2 is biological respinse tank body schematic diagram；
Fig. 3 is head space container schematic diagram；
Fig. 4 is head space container top view；
Fig. 5 is flow chart of the invention.
In figure: 1- constant water bath box；2- biological respinse tank body；3- head space container；4- high-pressure inert gas；5- indifferent gas
Body bottle valve；6- gas pressure reducer；The first connecting conduit of 7-；The first two-port valve of 8-；The second connecting conduit of 9-；10- first through hole；
The first sealing cover of 11-；The first seal washer of 12-；The second seal washer of 13-；The second sealing cover of 14-；The second through-hole of 15-；16-
Two two-port valves；17- leakage fluid dram；18- third connecting conduit；19- filter；The 4th connecting conduit of 20-；21- pressure gauge；22-
Three two-port valves；23- air hatch (contains seal washer)；24- exhaust outlet；The 4th two-port valve of 25-；26- culture solution；27- constant-flux pump；
The 5th connecting conduit of 28-.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiment is only a part of the embodiments of the present invention, instead of all the embodiments.Base
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts it is all its
His embodiment, shall fall within the protection scope of the present invention.
Embodiment one:
It is anti-according to a kind of Fig. 1-experimental provision of Organic biological gas output shown in Fig. 4, including temperature-controlling system, microorganism
Answer system, the gas storage circulatory system, pressurization and exhaust system, the first connecting tube 7, the second connecting tube 9 and the 4th connecting tube 20；It is described
The gas storage circulatory system include at least a head space container 3, one end of the microbial reaction system passes through the 4th connecting tube
20 enter in head space container 3 through 3 upper surface of head space container, and the other end of the microbial reaction system passes through the second connecting tube
9 connect with the bottom of the gas storage circulatory system；The pressurization and exhaust system is connected to the second connecting tube by the first connecting tube 7
On 9；The microbial reaction system and the gas storage circulatory system is placed in temperature-controlling system.
In actual use, microbial reaction system is to provide the field of observation microorganism and organic matter sample reaction process
Institute.Microbial reaction system of the invention can place the core sample that diameter is 42mm, can also place bulk sample or powdered
Sample, the sample scope of application are wider.The gas storage circulatory system is for providing microbial culture medium and generating the place of gas storage.
On the one hand being pressurized with exhaust system is the initial pressure tested when reacting in regulation and control system, on the other hand front-seat for experiment
Regulation pressurization and the pressure change in exhaust system, guarantee experiment safety when gas.Temperature-controlling system tests reaction temperature for controlling,
And the later period can be according to influence of the analysis of experimental data temperature factor to Biogas-generated Quantity under different experimental conditions.
The present invention passes through control variate method, the available temperature of difference, pressure, hydrodynamic force, microbe species, microbe quantity
The factors such as amount are to the output rate of biogas and the influence of total amount.The device of the invention can be used repeatedly, and accomplish section
Can be environmentally friendly, to reach efficiency maximization.
Embodiment two:
A kind of experimental provision of Organic biological gas output according to figure 1, with embodiment one the difference is that: institute
The temperature-controlling system stated is using water-bath temperature-controlling system.
The constant water bath box 1 of the preferably described water-bath temperature-controlling system use.
In actual use, water-bath temperature-controlling system is made of a big constant water bath box 1.The water bath is for controlling reality
Reaction temperature is tested, and the later period can be according to the analysis of experimental data temperature factor under different experimental conditions to Biogas-generated Quantity
Influence.Using the square technology case of constant water bath box 1, not only make installation and dismounting connecting conduit convenient, but also can also examine in real time
Look into biological respinse tank body and head space container be placed under water can check device leakproofness (there is bubble in gas leakage place), in experimentation
Temperature needed for reaction can also accurately be controlled.So that experiment is that at low cost, temperature control effect is good.The constant temperature of use of the invention
The model SC-160 of water bath 1, the interior band circulatory system of the bath of water bath, can be improved the uniformity of thermal field in bath, adopts
With PID temperature control technique, temperature accuracy is up to 0.05 DEG C.In actual use, the constant water bath box of other models can also be used
Or temperature control device, as long as temperature needed for reaction can be controlled accurately.
Embodiment three:
According to the experimental provision of Fig. 1 and a kind of Organic biological gas output shown in Fig. 2, exist with one difference of embodiment
In: the microbial reaction system includes at least biological respinse tank body 2, and one end of biological respinse tank body 2 passes through the second sealing
Washer 13 is removably connected with the second sealing cover 14, and the top of the second sealing cover 14 passes through the second through-hole 15 being provided with and third
One end of conduit 18 connects, and the other end of third conduit 18 is connect by filter 19 with the 4th connecting tube 20；Second sealing cover
14 lower part is provided with leakage fluid dram 17；The other end of biological respinse tank body 2 is removably connected with the by the first seal washer 12
One sealing cover 11, the top of the first sealing cover 11 pass through the first through hole 10 being provided with and connect with one end of the second connecting conduit 9, the
The other end of two connecting conduits 9 is connect by constant-flux pump 27 with 3 bottom of head space container.
Preferably the second two-port valve 16 is connected on the outside of the leakage fluid dram 17.
In actual use, biological respinse tank body 2 is to provide observation microorganism and organic matter sample in microbial reaction system
The place of product reaction process.This biological respinse tank body can straighten the core sample that diameter is 42mm, can also place bulk sample or powder
Last shape sample, the sample scope of application are wider.In such a system, 2 side of biological respinse tank body is dismountable first sealing cover 11
The injection port for adding the first seal washer 12 can be accomplished closed good when reaction and conveniently vary cleaning and repetitive cycling benefit
With the other side is dismountable second sealing cover 14 plus the second seal washer 13.The first closed cover 11 on biological respinse tank body 2
Top has first through hole 10 to connect with the second connecting conduit 9, and the second through-hole 15 of 2 right upper portion of biological respinse tank body connects third
Conduit 18, export reaction generate gas and microbial culture medium.Third connecting conduit 18 connects filter 19, to filter coal dust
Equal fine particles.2 lower part of biological respinse tank body is a leakage fluid dram 17, adds second two-port valve 16 to control biology on leakage fluid dram
The sealing of reacting tank body 2, while fluid sample is taken at any time with after for way in an experiment, it is organic to detect microbial degradation
Soluble substance changes of contents and microbial activity change in matter mechanism.2 two sides of biological respinse tank body are detachable closed
Lid, and added with seal washer, it not only ensure that the airtightness of experimental situation, but also facilitate the reality of Preparatory work of experiment stage and ending phase
Test instrument clean work；A filter is connected on the right side of biological respinse tank body, can prevent the fine particles such as coal dust from entering head space appearance
Device.
Example IV:
According to the experimental provision of Fig. 1, Fig. 3 and a kind of Organic biological gas output shown in Fig. 4, it is different from embodiment one it
Be in: the gas storage circulatory system further includes pressure gauge 21, the 5th connecting conduit 28, the 4th two-port valve 25 and third two-way
Valve 22；The pressure gauge 21 is connected to the upper surface of head space container 3, the 5th connecting conduit by third two-port valve 22
28 one end passes through 3 bottom surface of head space container and extends in head space container 3, and the other end of the 5th connecting conduit 28 and second connects
Connect the connection of conduit 9；The upper surface of head space container 3 is also provided with air hatch 23 and exhaust outlet 24, is connected with the four or two on exhaust outlet 24
Port valve 25.
In actual use, head space container 3 is to provide microbial culture medium and generates gas storage in the gas storage circulatory system
Place, the present embodiment use specification be 2L, internal diameter be 100mm cylindrical rustless steel container.In actual use, may be used
To use the container of different size according to actual needs.There are four 3 upper openings of head space container, is connection pressure gauge 21 respectively,
4th connecting conduit 20, air hatch 23, exhaust outlet 24；Lower openings have a mouth for connect the 5th connecting conduit 28.Wherein,
Pressure gauge 21 carries out the volume change for generating gas in the process to show experiment initial pressure and detect reaction, it is known that instead
The degree and gas generating amount that should be carried out, later period can also obtain microbe species, and the influence factors such as content are fast to gas is generated
The influence of rate.4th connecting conduit 20 is passed through in the culture solution in head space container 3, is connected to microbial reaction system, is on the one hand protected
It confirms to drain the air in connecting pipeline and microbial culture medium before testing after the opening of high-pressure inert gas 4, on the other hand in reality
The frequency of observation bubble judges the generation time and quantity of biogas during testing.Air hatch 23 be arranged seal washer 23, be
It is convenient to extract the gas in head space container by syringe in experimentation.Exhaust outlet 24 is to beat in the experiment incipient stage
It opens high-pressure inert gas and drains air outlet slit in reaction system, after being alternatively arranged as experiment, collect head space with collection airbag
The collection port of bulk gas in container 3.A constant-flux pump 27 is connected in 5th connecting conduit 28, the technical program is on the one hand
Can allow microbial culture medium one-way flow, can also when preparing experimental stage and being passed through inert gas, do not allow inert gas into
Enter head space container.5th connecting conduit 28 is in head space lower vessel portion access microbial culture medium.Top in the gas storage circulatory system
Empty container 3 can store the gas of generation；It, can be in initial pressure in display system and experiment added with pressure gauge on head space container 3
Pressure change reflects gas generating amount；Constant-flux pump is connected below head space container 3, provides circulation power for fluid in system.
Embodiment five:
A kind of experimental provision of Organic biological gas output according to figure 1, with embodiment one the difference is that: institute
The pressurization and exhaust system stated include high-pressure inert gas 4, inert gas bottle valve 5, gas pressure reducer 6 and the first two-port valve
8；The high-pressure inert gas 4 is connect with the input terminal of gas pressure reducer 6, and the output end of gas pressure reducer 6 and first connects
The connection of conduit 7 is connect, the first two-port valve 8 is connected in the first connecting conduit 7.
Gas preferably in the high-pressure inert gas 4 is high pure nitrogen.
In actual use, the gas in high-pressure inert gas 4 is high purity inert gas, to exclude microbial reaction system
System and the gas storage circulatory system and second, third, air and create the predominant gas of anaerobic experimental situation in the 4th connecting conduit.
6 one side of gas pressure reducer be regulation and control system in experiment reaction when initial pressure, on the other hand for experiment front exhaust when
Regulate and control the pressure change of high-pressure inert gas 4, guarantees experiment safety.First two-port valve 8 accesses first in 27 side of constant-flux pump
Connecting conduit 7, to connect the switch of pressurization Yu exhaust system and other experimental systems.Pressurization uses external with exhaust system
Initial pressure and anaerobism ring in 8 autonomous control system of one bottle of high-pressure inert gas 4, gas pressure reducer 6 and the first two-port valve
Border, in high-pressure inert gas 4 when hypotony can topping up or replacement inert gas bottle at any time, it is easy to operate, at
This is low, saves experiment consumptive material.Or else the gas used in high-pressure inert gas 4 can influence experimental precision for high pure nitrogen
On the basis of save the cost.In actual use, it also can according to need using the lazy of the helium of high-purity or other high-purities
Property gas, can also obtain same experiment effect.
Embodiment six:
According to a kind of Fig. 1-experimental provision of Organic biological gas output shown in Fig. 4, exist with two difference of embodiment
In: the constant water bath box 1 that the water-bath temperature-controlling system uses, the microbial reaction system include at least biological reactor
One end of body 2, biological respinse tank body 2 is removably connected with the second sealing cover 14, the second sealing by the second seal washer 13
The top of lid 14 passes through the second through-hole 15 being provided with and connect with one end of third conduit 18, and the other end of third conduit 18 passed through
Filter 19 is connect with the 4th connecting tube 20；The lower part of second sealing cover 14 is provided with leakage fluid dram 17；The other end of biological respinse tank body 2
It is removably connected with the first sealing cover 11 by the first seal washer 12, the top of the first sealing cover 11 passes through be provided with first
Through-hole 10 is connect with one end of the second connecting conduit 9, and the other end of the second connecting conduit 9 passes through constant-flux pump 27 and head space container 3
Bottom connection；The second two-port valve 16 is connected on the outside of the leakage fluid dram 17；The gas storage circulatory system further includes pressure gauge
21, the 5th connecting conduit 28, the 4th two-port valve 25 and third two-port valve 22；The pressure gauge 21 is connected by third two-port valve 22
It connects in the upper surface of head space container 3, one end of the 5th connecting conduit 28 passes through 3 bottom surface of head space container and extends to top
In empty container 3, the other end of the 5th connecting conduit 28 is connect with the second connecting conduit 9；The upper surface of head space container 3, which is also provided with, to be taken
Port 23 and exhaust outlet 24 are connected with the 4th two-port valve 25 on exhaust outlet 24；The pressurization includes that high pressure is lazy with exhaust system
Property gas bottle 4, inert gas bottle valve 5, gas pressure reducer 6 and the first two-port valve 8；The high-pressure inert gas 4 and gas
The input terminal of body pressure reducing valve 6 connects, and the output end of gas pressure reducer 6 is connect with the first connecting conduit 7, the connection of the first two-port valve 8
In the first connecting conduit 7.
Temperature, pressure and hydrodynamic condition when simulated formation original position microbial degradation of the present invention；Pass through constant temperature water bath system
System provides response of stratum temperature, simulates different strata pressures by the decompression valve regulation of high-pressure inert gas, passes through advection
Pump discharge controls to simulate underground difference flow rate.Biological respinse tank body can straighten the core sample that diameter is 42cm, can also be with
It places bulk sample or powdered samples, the sample scope of application is wider.Operation of the present invention method is simple, and economic cost is low, and
Analog result is closer to stratum actual conditions.
Embodiment seven:
A kind of test method of Organic biological gas output as shown in Figure 1 and Figure 5 is produced using a kind of Organic biological gas
Experimental provision out, includes the following steps
Step 1: utensil sterilization treatment
By biological respinse tank body 2, head space container 3, culture solution 26, removable seal lid 11,14, the company of filter 19, first
Connect conduit 7, the second connecting conduit 9, third connecting conduit 18, the 4th connecting conduit 20, the 5th connecting conduit 28, the first two-port valve
8, the second two-port valve 16, third two-port valve 22, the 4th two-port valve 25 carry out sterilization treatment；
Step 2: coalignment
Organic matter sample is put into through in the processed biological respinse tank body 2 of step 1, culture solution 26 is put into through step
In one processed head space container 3；
Step 3: device installation
All components handled well by step 1 and step 2 are attached；
Step 4: starter
Successively open pressurization and inert gas bottle valve 5, gas pressure reducer 6, the first two-port valve 8, the in exhaust system
Four two-port valves 25, it is continuous high purity inert gas 10-20 minutes logical, it is remaining in exclusion microbial reaction system, the gas storage circulatory system
Air, closes the 4th two-port valve 25, and regulating gas pressure reducing valve 6 turns off the first two-port valve 8, indifferent gas to setting strata pressure
Then body bottle valve 5 and gas pressure reducer 6 are opened constant water bath box 1 and 27 power switch of constant-flux pump, are made in head space container 3
Culture solution 26 circulates in the direction of the clock；
Step 5: gas and liquid are collected
In different time sections, head gas in head space container 3 is collected from air hatch 23；Biological respinse is acquired from leakage fluid dram 17
Liquid in device 2；
6th step, detection gas and liquid
The gas that step 5 is collected carries out gas component and methane concentration detection, and liquid carries out microbial diversity point
Analysis.
The present invention passes through utensil sterilization treatment, coalignment, device installation, starter, collection gas and liquid and inspection
Gas and six steps of liquid are surveyed, microorganism gasification experiment is preferably completed, strong technology is provided for experiment and research
It supports.
Embodiment eight:
A kind of test method of Organic biological gas output as shown in Figure 1 and Figure 5,
We are equipped with the culture solution 26 of a constant volume according to the method for methanogen culture solution first, by biological respinse tank body
2, head space container 3, culture solution 26, the first connecting conduit 7, the second connecting conduit 9, third connecting conduit 18, the 4th connecting conduit
20 and the 5th connecting conduit 28 and the first two-port valve 8, the second two-port valve 16, third two-port valve 22, the 4th two-port valve 25 be put into height
121 DEG C are carried out in warm steam sterilization pan to sterilize 20 minutes, are then taken out after laboratory apparatus is cooling, are put on clean work station and fill
Sample simultaneously connects each system.
Firstly, the core sample of certain mine internal diameter 42mm, length 300mm are packed into biological respinse tank body 2, then may be used
The first sealing cover 11 and the second sealing cover 14 of disassembly are separately mounted to 2 two sides of biological respinse tank body, with third connecting conduit 18
Filter 19 and the second through-hole 15 are connected, then the pressure gauge 21, seal washer 23, third two-port valve and of head space container are installed
Four two-port valves, finally with the first connecting conduit 7, the second connecting conduit 9, third connecting conduit 18, the 4th connecting conduit the 20, the 5th
Successively by high-pressure inert gas, biological respinse tank body 2, filter 19, head space container 3 and constant-flux pump 27 connect connecting conduit 28
It connects, experimental provision is completed.
Specific experiment operating procedure are as follows:
1, the 4th two-port valve 25 is successively opened, the first two-port valve 8, inert gas bottle valve 5, gas pressure reducer 6 are adjusted to
Outlet stream pressure appropriate, blown inert gas 10 minutes.
2, air in microbial reaction system and the gas storage circulatory system is drained, is then shut off the 4th two-port valve 25, and adjust
6 outlet pressure of gas pressure reducer is to stratum simulated pressure 1MPa.
3, after system pressure balance, the first two-port valve 8, inert gas bottle valve 5, gas pressure reducer 6 are closed.
4, it opens water bath used by the present embodiment to switch, temperature is adjusted to 35 DEG C, opens advection switch pump, and flow velocity is adjusted to
0.1ml/min extracted test of a certain amount of gas for gas component, experiment with syringe from air hatch 23 every 3-5 days
After device continues 60-90 days, reaction terminates, and acquires residual gas in head space container from exhaust outlet 24, acquires and give birth to from leakage fluid dram 17
Remaining liq is tested for sundry item in object reacting tank body 2.
5, test after successively close water bath and advection pumping source switch, according to assembling laboratory apparatus the step of, according to
Secondary each experiment accessory of disassembly, after cleaning up, for experiment next time.
In conclusion the present invention passes through temperature-controlling system, microbial reaction system, the gas storage circulatory system and pressurization and exhaust system
A kind of experimental provision for the Organic biological gas output formed of uniting by utensil sterilization treatment, coalignment, device installation, opens
Dynamic device collects six steps of gas and liquid and detection gas and liquid, controls experimentation accurately needed for reaction
Temperature, facilitate the laboratory apparatus cleaning of Preparatory work of experiment stage and ending phase；Biological respinse tank body connects a filtering
Device can prevent the fine particles such as coal dust from entering head space container；Pressurization uses one bottle of external high-pressure inert gas with exhaust system
Initial pressure and anaerobic environment in bottle, pressure reducing valve and the first two-port valve autonomous control system, the inert gas pressure in the bottle are too low
When can at any time topping up or replacement inert gas bottle, it is easy to operate, it is at low cost, save experiment consumptive material.Gas storage cyclic system
System can store the gas of generation；It, can be with initial in display system added with pressure gauge on the head space container in the gas storage circulatory system
Pressure change in pressure and experiment, reflects gas generating amount；Constant-flux pump is connected below head space container, is provided and is followed for fluid in system
Gyration power.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
It is to be appreciated that the directional instruction such as up, down, left, right, before and after ... of institute is only used in the embodiment of the present invention
Relative positional relationship, the motion conditions etc. in the case where a certain particular pose is as shown in the picture between each component are explained, if this is specific
When posture changes, then directionality instruction also correspondingly changes correspondingly.
In addition, the description for being related to " first ", " second " etc. in the present invention is used for description purposes only, and should not be understood as referring to
Show or imply its relative importance or implicitly indicates the quantity of indicated technical characteristic." first ", " are defined as a result,
Two " feature can explicitly or implicitly include at least one of the features.
In the absence of conflict, those skilled in the art can according to the actual situation will be relevant in above-mentioned each example
Technical characteristic is combined with each other, and to reach corresponding technical effect, will not repeat them here particularly for various combined situations.
The above, near-sighted presently preferred embodiments of the present invention, the present invention be not intended to be limited to it is shown in this article this
A little embodiments, 20 will meet the widest scope consistent with principles disclosed herein and novel features.According to this hair
Bright technical spirit any simple modification, equivalent change and modification to the above embodiments, still fall within the technology of the present invention
In the range of scheme.
Claims (10)
1. a kind of experimental provision of Organic biological gas output, it is characterised in that: including temperature-controlling system, microbial reaction system,
The gas storage circulatory system, pressurization and exhaust system, the first connecting tube (7), the second connecting tube (9) and the 4th connecting tube (20)；It is described
The gas storage circulatory system include at least a head space container (3), one end of the microbial reaction system passes through the 4th connection
Pipe (20) enters in head space container (3) through head space container (3) upper surface, and the other end of the microbial reaction system passes through the
Two connecting tubes (9) are connect with the bottom of the gas storage circulatory system；The pressurization is connect with exhaust system by the first connecting tube (7)
On the second connecting tube (9)；The microbial reaction system and the gas storage circulatory system is placed in temperature-controlling system.
2. a kind of experimental provision of Organic biological gas output as described in claim 1, it is characterised in that: the temperature control system
System is using water-bath temperature-controlling system.
3. a kind of experimental provision of Organic biological gas output as described in claim 1, it is characterised in that: the water-bath control
The constant water bath box (1) that warm system uses.
4. a kind of experimental provision of Organic biological gas output as described in claim 1, it is characterised in that: the microorganism
Reaction system includes at least biological respinse tank body (2), and one end of biological respinse tank body (2) is removable by the second seal washer (13)
That unloads is connected with the second sealing cover (14), and the top of the second sealing cover (14) passes through the second through-hole (15) and third conduit being provided with
(18) one end connection, the other end of third conduit (18) are connect by filter (19) with the 4th connecting tube (20)；Second is close
The lower part of capping (14) is provided with leakage fluid dram (17)；The other end of biological respinse tank body (2) is removable by the first seal washer (12)
That unloads is connected with the first sealing cover (11), and the top of the first sealing cover (11) passes through the first through hole (10) being provided with and connect with second
One end of conduit (9) connects, and the other end of the second connecting conduit (9) is connect by constant-flux pump (27) with head space container (3) bottom.
5. a kind of experimental provision of Organic biological gas output as claimed in claim 4, it is characterised in that: the leakage fluid dram
(17) outside is connected with the second two-port valve (16).
6. a kind of experimental provision of Organic biological gas output as described in claim 1, it is characterised in that: the gas storage follows
Loop system further includes pressure gauge (21), the 5th connecting conduit (28), the 4th two-port valve (25) and third two-port valve (22)；Described
Pressure gauge (21) is connected to the upper surface of head space container (3), the 5th connecting conduit (28) by third two-port valve (22)
One end pass through head space container (3) bottom surface and extend in head space container (3), the other end of the 5th connecting conduit (28) and the
Two connecting conduits (9) connection；The upper surface of head space container (3) is also provided with air hatch (23) and exhaust outlet (24), exhaust outlet (24)
On be connected with the 4th two-port valve (25).
7. a kind of experimental provision of Organic biological gas output as described in claim 1, it is characterised in that: the pressurization with
Exhaust system includes high-pressure inert gas (4), inert gas bottle valve (5), gas pressure reducer (6) and the first two-port valve (8)；
The high-pressure inert gas (4) is connect with the input terminal of gas pressure reducer (6), the output end of gas pressure reducer (6) and
One connecting conduit (7) connection, the first two-port valve (8) are connected on the first connecting conduit (7).
8. a kind of experimental provision of Organic biological gas output as claimed in claim 7, it is characterised in that: the high pressure is lazy
Property gas bottle (4) in gas be high pure nitrogen.
9. a kind of experimental provision of Organic biological gas output as described in claim 1, it is characterised in that: the water-bath control
The constant water bath box (1) that warm system uses, the microbial reaction system include at least biological respinse tank body (2), and biology is anti-
One end of tank body (2) is answered removably to be connected with the second sealing cover (14), the second sealing cover by the second seal washer (13)
(14) top passes through the second through-hole (15) being provided with and connect with one end of third conduit (18), the other end of third conduit (18)
It is connect by filter (19) with the 4th connecting tube (20)；The lower part of second sealing cover (14) is provided with leakage fluid dram (17)；It is biological anti-
The other end of tank body (2) is answered removably to be connected with the first sealing cover (11), the first sealing cover by the first seal washer (12)
(11) top passes through the first through hole (10) being provided with and connect with one end of the second connecting conduit (9), the second connecting conduit (9)
The other end is connect by constant-flux pump (27) with head space container (3) bottom；The second two-way is connected on the outside of the leakage fluid dram (17)
Valve (16)；The gas storage circulatory system further includes pressure gauge (21), the 5th connecting conduit (28), the 4th two-port valve (25) and
Three two-port valves (22)；The pressure gauge (21) is connected to the upper surface of head space container (3) by third two-port valve (22), described
One end of the 5th connecting conduit (28) pass through head space container (3) bottom surface and extend in head space container (3), the 5th connection is led
The other end of pipe (28) is connect with the second connecting conduit (9)；The upper surface of head space container (3) is also provided with air hatch (23) and exhaust
Mouthful (24) are connected with the 4th two-port valve (25) on exhaust outlet (24)；The pressurization and exhaust system includes high-pressure inert gas
Bottle (4), inert gas bottle valve (5), gas pressure reducer (6) and the first two-port valve (8)；The high-pressure inert gas (4)
It being connect with the input terminal of gas pressure reducer (6), the output end of gas pressure reducer (6) is connect with the first connecting conduit (7), and the one or two
Port valve (8) is connected on the first connecting conduit (7).
10. a kind of test method of the experimental provision of Organic biological gas output as claimed in claims 1-9, feature exist
In: include the following steps
Step 1: utensil sterilization treatment
By biological respinse tank body (2), head space container (3), culture solution (26), the first sealing cover (11), the second sealing cover (14), mistake
Filter (19), the first connecting conduit (7), the second connecting conduit (9), third connecting conduit (18), the 4th connecting conduit (20),
Five connecting conduits (28), the first two-port valve (8), the second two-port valve (16), third two-port valve (22), the 4th two-port valve (25) carry out
Sterilization treatment；
Step 2: coalignment
Organic matter sample is put into through in the processed biological respinse tank body (2) of step 1, culture solution (26) are put into through step
In one processed head space container (3)；
Step 3: device installation
All components handled well by step 1 and step 2 are attached；
Step 4: starter
Successively open pressurization with exhaust system in inert gas bottle valve (5), gas pressure reducer (6), the first two-port valve (8),
4th two-port valve (25), it is continuous high purity inert gas 10-20 minutes logical, in exclusion microbial reaction system, the gas storage circulatory system
Surplus air, closes the 4th two-port valve (25), and regulating gas pressure reducing valve (6) turns off the first two-port valve to setting strata pressure
(8), inert gas bottle valve (5) and gas pressure reducer (6) are then opened temperature-controlling system and constant-flux pump (27) power switch, are made
Head space container (3) interior culture solution (26) circulates in the direction of the clock；
Step 5: gas and liquid are collected
In different time sections, head space container (3) interior head gas is collected from air hatch (23)；It is anti-from leakage fluid dram (17) acquisition biology
Answer the liquid in device (2)；
6th step, detection gas and liquid
The gas that step 5 is collected carries out gas component and methane concentration detection, and liquid carries out Analysis of Microbial Diversity.
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