CN114594228A - Microorganism-driven in-situ ecological simulation device and method for gathering and storing biogas - Google Patents
Microorganism-driven in-situ ecological simulation device and method for gathering and storing biogas Download PDFInfo
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- 238000004088 simulation Methods 0.000 title claims abstract description 173
- 238000011065 in-situ storage Methods 0.000 title claims abstract description 60
- 238000000034 method Methods 0.000 title claims abstract description 29
- 244000005700 microbiome Species 0.000 title claims abstract description 20
- 239000002689 soil Substances 0.000 claims abstract description 23
- 238000003825 pressing Methods 0.000 claims abstract description 17
- 239000011148 porous material Substances 0.000 claims abstract description 11
- 230000002776 aggregation Effects 0.000 claims abstract description 7
- 238000004220 aggregation Methods 0.000 claims abstract description 7
- 238000005070 sampling Methods 0.000 claims description 58
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 39
- 238000007789 sealing Methods 0.000 claims description 19
- 239000004927 clay Substances 0.000 claims description 18
- 238000009825 accumulation Methods 0.000 claims description 13
- 230000008569 process Effects 0.000 claims description 13
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- 150000002500 ions Chemical class 0.000 claims description 7
- 239000004744 fabric Substances 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims description 3
- 239000012780 transparent material Substances 0.000 claims description 2
- 230000009919 sequestration Effects 0.000 claims 2
- 230000000813 microbial effect Effects 0.000 claims 1
- 230000005012 migration Effects 0.000 abstract description 4
- 238000013508 migration Methods 0.000 abstract description 4
- 230000000035 biogenic effect Effects 0.000 abstract 2
- 239000004576 sand Substances 0.000 description 6
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- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 4
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- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 239000007832 Na2SO4 Substances 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
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- 239000011521 glass Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
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- 235000010344 sodium nitrate Nutrition 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/24—Earth materials
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/30—Landfill technologies aiming to mitigate methane emissions
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Abstract
The invention aims to provide a microbe-driven biogas aggregation in-situ ecological simulation device and method, which comprises a simulation column, wherein the simulation column is of a hollow cylindrical structure with openings at two ends, a simulation column top cover is arranged at the top of the simulation column, the simulation column top cover is hermetically connected with the simulation column through threads, an air pump is arranged at the upper part of the simulation column top cover, an axial force rod is arranged at the lower part of the air pump and penetrates through the simulation column top cover, an axial force pressing plate is arranged at the bottom of the axial force rod, and a plurality of through holes are formed in the axial force pressing plate; the simulation column bottom is provided with a simulation column bottom cover, the simulation column bottom cover is hermetically connected with the simulation column through threads, one side of the simulation column bottom cover is provided with a bottom pressure controller interface, and the bottom of the simulation column bottom cover is provided with a base. The utility model provides a biogenic gas that microorganism driven gathers and becomes to hide normal position ecological simulation device, the migration and the gathering of simulation biogenic gas can simulate different normal position soil stress condition and soil body normal position pore state in the simulation post, and the normal position environment that the great degree reappears the microorganism and locates.
Description
Technical Field
The invention relates to the fields related to engineering safety and ecological simulation, in particular to a microorganism-driven biogas aggregation and accumulation in-situ ecological simulation device and method.
Background
In the engineering construction of coastal areas and middle and lower reaches of Yangtze river in east China, shallow gas-bearing soil layers with shallow burial depth are encountered for many times, and gas reservoirs are different in scale, thickness and air pressure and mainly exist in sand layers containing shells. In part of fields, after gas is released by drilling in the early stage, the phenomena of gas injection and sand overflow still occur when secondary drilling is carried out for a plurality of days, which shows that the reservoir connectivity is better and the reservoir can be transported for the second time. Examples of major economic losses due to engineering accidents and engineering accidents caused by the subsidence and breakage of tunnels, foundation pits, open caisson and hydraulic structures due to the release of shallow gas are frequently reported.
The methane gas is colorless, tasteless, inflammable and explosive, and the gas reservoir is buried in shallow depth, is widely distributed and has large gas quantity, so that adverse effects are certainly brought to subway engineering under construction, and secondary gas production after gas discharge treatment still threatens subsequent safety problems of the engineering, so that the strength and scale development of model test research on secondary gas production of the shallow gas reservoir under the in-situ condition are urgently needed.
The existing research usually estimates the gas generation potential according to the organic matter content, does not consider the consumption of anaerobic oxidizing bacteria to methane gas, does not consider the migration of gas in a porous medium before the gas is gathered, and lacks a model simulation which is more in line with the actual situation in the whole process.
Disclosure of Invention
The invention aims to provide a microorganism-driven biogas aggregation and accumulation in-situ ecological simulation device and method, so as to fill up the technical gap of performing model simulation on the process.
In order to achieve the purpose, the invention adopts the following technical scheme:
a microbe-driven in-situ ecological simulation device for gathering biogas comprises a simulation column, wherein the simulation column is of a hollow tubular structure with two open ends, a simulation column top cover is arranged at the top of the simulation column, the simulation column top cover is hermetically connected with the simulation column through threads, a rubber gasket can be additionally arranged between the simulation column top cover and the simulation column to further improve the air tightness, an air pump is arranged at the upper part of the simulation column top cover, an air pump shell and the simulation column top cover can be of an integrated structure, an axial force rod is arranged at the lower part of the air pump and penetrates through the simulation column top cover, a sealing structure such as a rubber sealing strip is arranged between the axial force rod and the simulation column top cover, the influence on the air tightness of the whole simulation device is reduced, an axial force pressing plate is arranged at the bottom of the axial force rod and provided with a plurality of through holes so as to ensure that the biogas is matched with filter paper when in use, so that gas or liquid can penetrate through the axial force pressing plate, but solid substances such as clay, sand and the like cannot penetrate through the axial force pressing plate.
The simulation column bottom cover is arranged at the bottom of the simulation column and is hermetically connected with the simulation column through threads, a rubber gasket can be additionally arranged between the simulation column bottom cover and the simulation column to further improve the air tightness, a bottom pressure controller interface is arranged at one side of the simulation column bottom cover and is of an integrated structure with the simulation column bottom cover, a controller interface valve is arranged on the bottom pressure controller interface, and a base is arranged at the bottom of the simulation column bottom cover and is used for ensuring the stability of the whole device when the device is placed;
a back pressure controller interface is arranged on one side of the upper part of the simulation column, the back pressure controller interface and the simulation column are of an integrated structure, a controller interface valve is arranged on the back pressure controller interface, and a plurality of vertically arranged sampling ports are arranged on one side of the simulation column between the back pressure controller interface and the bottom end of the simulation column;
the inside lower part of simulation post is equipped with the snap ring, and the snap ring that indicates here can be complete annular, also can be by a plurality of spacing points constitution, and its main objective is in order to spacing to the porous disk, prevents it and drops, snap ring upper portion is equipped with the porous disk, the porous disk is equipped with a plurality of through-hole to under the cooperation of filter paper, make gas or liquid can see through the porous disk when guaranteeing to use, but solid-state material such as clay, sand can't see through.
Furthermore, the simulation column is made of transparent materials, so that the condition inside the simulation column can be observed in real time conveniently in the simulation process.
Furthermore, the middle part of one side of the simulation column is provided with a barometer for measuring the air pressure value in the simulation column when in use, the position of the barometer is arranged at the middle part of the simulation column and is not on the same side with the sampling port, so that the barometer can be kept on the same layer with sandy soil in the simulation column when in use, and the sampling through the sampling port can be guaranteed not to be influenced.
Further, arbitrary the sample connection includes the sample body, the sample body is close to the one end of simulation post is equipped with the sample valve, the inside sample filter membrane that is equipped with of sample body, the sample filter membrane can make gas or liquid can see through, but solid-state material such as clay, sand can not see through, the sample body is kept away from the one end of simulation post is equipped with the seal membrane, the sample body is kept away from the one end outside of simulation post is equipped with sealing nut.
A method for simulating in-situ ecology of gathering of biogas driven by microorganisms into a reservoir is based on the device for simulating in-situ ecology of gathering of biogas driven by microorganisms, and simultaneously needs to complete soil sampling and data acquisition of a simulated plot before simulation so as to complete simulation, wherein the soil to be sampled comprises clay at the simulated plot, namely in-situ clay, and sandy soil at the simulated plot, namely in-situ sandy soil, and the data to be acquired comprises in-situ soil stress at the simulated plot, in-situ soil pore pressure level, environmental temperature, in-situ ionic component and concentration of water in the soil, in-situ pore water content and the like, and the method comprises the following steps:
s01, connecting the simulation column to the simulation column bottom cover, placing the water permeable plate on the clamping ring, and placing filter paper on the water permeable plate;
s02, sequentially placing in-situ clay, in-situ sandy soil and in-situ clay into the simulation column from the opening at the top end of the simulation column, and placing filter paper above the in-situ clay at the top layer;
s03, connecting the top cover of the simulation column to the top of the simulation column through threads, so that the inside of the simulation column is in a sealed state;
s04, introducing airless water with the concentration consistent with the in-situ ion concentration into the simulation column for seepage and exhaust until the back pressure in the simulation column reaches the in-situ pore pressure level, and forming an anaerobic environment in the simulation column;
s05, enabling the axial force pressing plate to press the filter paper placed above the in-situ clay on the top layer through adjusting the air pump, and enabling the force exerted on the filter paper by the axial force pressing plate to reach the stress level of in-situ soil;
s06, placing the simulation device in a temperature control box, adjusting the temperature to be approximate to the original ambient temperature, wherein the simulation device in the specification is the microorganism-driven biogas aggregation in-situ ecological simulation device;
s07, respectively taking water samples from the sampling ports, and detecting the concentration of biogas in the water samples through an instrument;
and S08, periodically and repeatedly completing the S07, recording the concentration of the detected biogas in the water sample, drawing a concentration-time relation curve, and simultaneously, periodically observing the change condition of sand soil or clay in the simulation column to analyze the rate of biogas generation and aggregation and the migration direction.
Further, in the foregoing S04 process, the specific steps include:
s041, using two pressure volume controllers as a back pressure controller and a bottom pressure controller respectively, and injecting airless water with the concentration consistent with that of the in-situ ions respectively;
s042, connecting the back pressure controller with an interface of the back pressure controller through a pipeline, and connecting the bottom pressure controller with the interface of the bottom pressure controller through a pipeline;
s043, setting the pressure of the back pressure controller to be 0KPa, setting the pressure of the bottom pressure controller to be 100KPa, carrying out seepage drainage from bottom to top under the action of pressure difference, considering that the air exhaust is basically completed when the water inflow in the back pressure controller exceeds 2 times of the original position pore volume, and considering that the air exhaust is completed when the water inflow in the back pressure controller is more than or equal to two times of the pore volume of the soil body in the test process, namely the water inflow fully replaces the air and the pore water in the soil pore.
Further, in the aforementioned S06 process, the temperature setting range of the temperature control box is generally 10 ℃ to 30 ℃, the specific temperature refers to the actual temperature of the simulated place, and the simulation device is wrapped with black shading cloth before being placed in the temperature control box, so as to simulate the dark in-situ soil layer condition.
Further, sample connection quantity is 3, wherein from top to bottom the second sample connection is located the midpoint position of simulation post one side, and the distance of the second sample connection of remaining two sample connection utensil equals.
Further, in the foregoing S07 process, the specific steps include:
s071, selecting a vacuum sampling tube for water sample collection;
s072, opening the sealing nut at the sampling port of a water sample to be collected, and adjusting the sampling valve to an open state to enable the water sample in the simulation column to enter the sampling pipe body of the sampling port;
s073, inserting a needle head of the vacuum sampling pipe into the sampling pipe body after penetrating through the sealing film at the sampling port of a water sample to be collected;
and S074, after sampling is finished, pulling out the needle head of the vacuum sampling tube, and screwing the sealing nut to ensure that the sealing state is maintained in the simulation column.
Further, in the process of S08, the sampling step of S07 is performed every 24 hours, the simulation of the first stage lasts for 8 days, and the sampling interval is adjusted according to the requirements of the related research or related engineering.
In conclusion, the beneficial technical effects of the invention are as follows:
the application provides a key physical simulation means for disclosing gas surge and catastrophe mechanisms in coastal urban strata widely distributed in China and containing high-pressure biogas reservoirs.
The device and the method have the advantages that the biogas driven by the microorganisms is gathered to form the hidden in-situ ecological simulation device, the migration and gathering of the biogas are simulated, different in-situ soil stress conditions and soil body in-situ pore states can be simulated in the simulation column, the in-situ environment where the microorganisms are located is reproduced to a large extent, and the device and the method are simple to operate and are not prone to errors.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a front view of embodiment 1 of the present invention;
FIG. 2 is a sectional view showing a state in which the present invention is used in example 1;
FIG. 3 is a cross-sectional view of a sampling port portion in example 1 of the present invention.
Reference numerals:
1. simulation column 2, simulation column top cover 3 and simulation column bottom cover
11. Back pressure controller interface 12, sampling port 13, barometer
14. Snap ring 15, porous disk
21. Air pump 22, axial force rod 23 and axial force pressing plate
31. Bottom pressure controller interface 32, base
121. Sampling tube 122, sampling valve 123, sampling filter membrane
124. Sealing film 125, sealing nut
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
Example 1
A microbe-driven biogas aggregation in-situ ecological simulation device comprises a simulation column 1, wherein the simulation column 1 is of a hollow cylindrical structure with openings at two ends, a simulation column top cover 2 is arranged at the top of the simulation column 1, the simulation column top cover 2 is hermetically connected with the simulation column 1 through threads, an air pump 21 is arranged at the upper part of the simulation column top cover 2, an axial force rod 22 is arranged at the lower part of the air pump 21, the axial force rod 22 penetrates through the simulation column top cover 2, an axial force pressing plate 23 is arranged at the bottom of the axial force rod 21, and the axial force pressing plate 23 is provided with a plurality of through holes;
One side of the upper part of the simulation column 1 is provided with a back pressure controller interface 11, and one side of the simulation column 1 is provided with a plurality of vertically arranged sampling ports 12 between the back pressure controller interface 11 and the bottom end of the simulation column 1.
The inside lower part of simulation post 1 is equipped with snap ring 14, and snap ring 14 upper portion is equipped with porous disk 15, and porous disk 15 is equipped with a plurality of through-hole.
Wherein, the simulation column 1 is made of transparent organic glass. The middle part of one side of the simulation column 1 is provided with a barometer 13.
The 12 quantity of sample connection is 3, and arbitrary sample connection 12 is including sample body 121, and the one end that sample body 121 is close to analog column 1 is equipped with sampling valve 122, and sample body 121 is inside to be equipped with sample filter membrane 123, and the one end that analog column 1 was kept away from to sample body 121 is equipped with seal membrane 124, and the one end outside that analog column 1 was kept away from to sample body 121 is equipped with sealing nut 125.
Example 2
A microorganism-driven biogas accumulation in-situ ecological simulation method applies the microorganism-driven biogas accumulation in-situ ecological simulation device in the embodiment 1, and comprises the following steps:
s01, connecting the simulation column to the simulation column bottom cover, placing the water permeable plate on the clamping ring, and placing filter paper on the water permeable plate;
s02, placing a 15 cm-thick refrigerated fresh-keeping clay column, a 5 cm-thick refrigerated fresh-keeping sandy soil column and a 15 cm-thick refrigerated fresh-keeping clay column into the simulation column in sequence from an opening at the top end of the simulation column, and placing filter paper above the in-situ clay on the top layer;
s03, connecting the top cover of the simulation column to the top of the simulation column through threads to enable the interior of the simulation column to be in a sealed state;
s04, preparing a solution with in-situ sulfate ion and nitrate ion concentrations by using quantitative Na2SO4 and NaNO3 powder, introducing prepared airless water with the ion concentration consistent with that of the in-situ ions into a simulation column for seepage and exhaust until the back pressure in the simulation column reaches the in-situ pore pressure level, and forming an anaerobic environment in the simulation column;
s05, pressing the filter paper placed above the in-situ clay on the top layer by the axial force pressing plate through adjusting the air pump, and enabling the force exerted on the filter paper by the axial force pressing plate to reach the stress level of in-situ soil;
s06, placing the simulation device in a temperature control box, setting the temperature to be 25 ℃, and wrapping the simulation device with black shading cloth before placing the simulation device in the temperature control box to simulate dark in-situ soil layer conditions;
s07, respectively taking water samples from each sampling port, and detecting the concentration of biogas in the water samples through an instrument;
and S08, repeating the step S07 every 24 hours for 8 times, recording the concentration of the detected biogas in the water sample, and drawing a concentration-time relation curve.
In the S04 process, the specific steps include:
s041, using two pressure volume controllers as a back pressure controller and a bottom pressure controller respectively, and injecting airless water with the concentration consistent with that of the in-situ ions respectively;
s042, connecting the back pressure controller with a back pressure controller interface through a pipeline, and connecting the bottom pressure controller with a bottom pressure controller interface through a pipeline;
s043, setting the pressure of the back pressure controller to be 0KPa and setting the pressure of the bottom pressure controller to be 100KPa
In the S07 process, the specific steps include:
s071, selecting a vacuum sampling tube for water sample collection;
s072, opening a sealing nut at a sampling port of a water sample to be collected, and adjusting a sampling valve to an open state to enable the water sample in the simulation column to enter a sampling pipe body of the sampling port;
s073, inserting a needle of a vacuum sampling tube into a sampling tube body after penetrating a sealing film at a sampling port of a water sample to be collected;
and S074, after sampling is finished, pulling out the needle head of the vacuum sampling tube, and screwing the sealing nut.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and these modifications or substitutions do not depart from the spirit of the corresponding technical solutions of the embodiments of the present invention.
Claims (10)
1. A microbe-driven biogas aggregation in-situ ecological simulation device is characterized by comprising a simulation column, wherein the simulation column is of a hollow cylindrical structure with two open ends, a simulation column top cover is arranged at the top of the simulation column, the simulation column top cover is hermetically connected with the simulation column through threads, an air pump is arranged at the upper part of the simulation column top cover, an axial force rod is arranged at the lower part of the air pump and penetrates through the simulation column top cover, an axial force pressing plate is arranged at the bottom of the axial force rod, and a plurality of through holes are formed in the axial force pressing plate;
the bottom of the simulation column is provided with a simulation column bottom cover, the simulation column bottom cover is hermetically connected with the simulation column through threads, one side of the simulation column bottom cover is provided with a bottom pressure controller interface, and the bottom of the simulation column bottom cover is provided with a base;
a back pressure controller interface is arranged on one side of the upper part of the simulation column, and a plurality of vertically arranged sampling ports are arranged on one side of the simulation column between the back pressure controller interface and the bottom end of the simulation column;
the inside lower part of simulation post is equipped with the snap ring, snap ring upper portion is equipped with the porous disk, the porous disk is equipped with a plurality of through-hole.
2. The microorganism-driven biogas accumulation and sequestration in-situ ecological simulation device according to claim 1, wherein the simulation column is made of transparent material.
3. The microorganism-driven biogas accumulation and sequestration in-situ ecological simulation device according to claim 2, wherein a barometer is arranged in the middle of one side of the simulation column.
4. The in-situ biogas accumulation and accumulation simulation apparatus according to claim 3, wherein any one of the sampling ports comprises a sampling tube, a sampling valve is disposed at an end of the sampling tube close to the simulation column, a sampling filter membrane is disposed inside the sampling tube, a sealing membrane is disposed at an end of the sampling tube far away from the simulation column, and a sealing nut is disposed outside an end of the sampling tube far away from the simulation column.
5. A microorganism-driven biogas accumulation in-situ ecological simulation method based on the microorganism-driven biogas accumulation in-situ ecological simulation device of claim 4, which is characterized by comprising the following steps:
s01, connecting the simulation column to the simulation column bottom cover, placing the water permeable plate on the clamping ring, and placing filter paper on the water permeable plate;
s02, sequentially placing in-situ clay, in-situ sandy soil and in-situ clay into the simulation column from the opening at the top end of the simulation column, and placing filter paper above the in-situ clay at the top layer;
s03, connecting the top cover of the simulation column to the top of the simulation column through threads, so that the inside of the simulation column is in a sealed state;
s04, introducing airless water with the concentration consistent with the in-situ ion concentration into the simulation column for seepage and exhaust until the back pressure in the simulation column reaches the in-situ pore pressure level, and forming an anaerobic environment in the simulation column;
s05, enabling the axial force pressing plate to press the filter paper placed above the in-situ clay on the top layer through adjusting the air pump, and enabling the force exerted on the filter paper by the axial force pressing plate to reach the stress level of in-situ soil;
s06, placing the simulation device in a temperature control box;
s07, respectively taking water samples from the sampling ports, and detecting the concentration of biogas in the water samples through an instrument;
and S08, periodically and repeatedly completing the step S07, recording the concentration of the detected biogas in the water sample, and drawing a curve of the relationship between the concentration and the time.
6. The in-situ ecological simulation method for accumulation of biogas driven by microorganisms according to claim 5, wherein in the S04 process, the specific steps comprise:
s041, using two pressure volume controllers as a back pressure controller and a bottom pressure controller respectively, and injecting airless water with the concentration consistent with that of the in-situ ions respectively;
s042, connecting the back pressure controller with an interface of the back pressure controller through a pipeline, and connecting the bottom pressure controller with the interface of the bottom pressure controller through a pipeline;
s043, setting the pressure of the back pressure controller to be 0KPa, and setting the pressure of the bottom pressure controller to be 100 KPa.
7. The in-situ ecological simulation method for gathering biogas driven by microorganisms into a reservoir as recited in claim 5, wherein in the S06 process, the temperature control box is set at a temperature ranging from 10 ℃ to 30 ℃, and the simulation device is wrapped by black shading cloth before being placed in the temperature control box.
8. The method of claim 5, wherein the number of sampling ports is 3, and the second sampling port from top to bottom is located at the midpoint of one side of the simulation column.
9. The in-situ ecological simulation method for accumulation of biogas driven by microorganisms according to claim 8, wherein in the S07 process, the specific steps comprise:
s071, selecting a vacuum sampling tube for water sample collection;
s072, opening the sealing nut at the sampling port of a water sample to be collected, and adjusting the sampling valve to an open state to enable the water sample in the simulation column to enter the sampling pipe body of the sampling port;
s073, inserting a needle head of the vacuum sampling pipe into the sampling pipe body after penetrating through the sealing film at the sampling port of a water sample to be collected;
and S074, after sampling is finished, pulling out the needle head of the vacuum sampling tube, and screwing the sealing nut.
10. The in-situ bio-simulation method for microbial-driven biogas accumulation according to claim 5, wherein the sampling step of S07 is performed every 24 hours during the S08.
Priority Applications (1)
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1851457A (en) * | 2006-05-19 | 2006-10-25 | 中国矿业大学 | Urban house refuse geologic landfill analogue experiment method and device |
| DE102009038017A1 (en) * | 2009-08-10 | 2011-02-24 | Bgd Boden- Und Grundwasserlabor Gmbh Dresden | Determination of microbial decomposition rates of first-order in fluid-saturated or fluid-partially saturated porous media of the soil- and groundwater areas, comprises flowing a water sample to be examined through successive reactors |
| CN104326558A (en) * | 2014-11-11 | 2015-02-04 | 南京大学 | Device for simulating in-situ river course sediment anaerobic ammoxidation process, and use method and application thereof |
| CN104777011A (en) * | 2015-03-26 | 2015-07-15 | 浙江大学 | Beach deposit collection and in situ ecology stimulation apparatus and method thereof |
| CN205353052U (en) * | 2016-01-25 | 2016-06-29 | 河海大学 | Mixed landfill post biochemical degradation test device of city domestic waste and mud |
| CN107358858A (en) * | 2017-07-18 | 2017-11-17 | 山西蓝焰煤层气集团有限责任公司 | Coal seam Biogenetic Gas Reservoir process simulation device and experimental method |
| CN109337809A (en) * | 2018-11-16 | 2019-02-15 | 曲阜师范大学 | A kind of early warning regulation method strengthened based on the unbalance microbial resistance of anaerobic digestion |
| CN109459341A (en) * | 2018-12-25 | 2019-03-12 | 西安科技大学 | A kind of experimental provision and method of Organic biological gas output |
-
2022
- 2022-01-27 CN CN202210098747.XA patent/CN114594228B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1851457A (en) * | 2006-05-19 | 2006-10-25 | 中国矿业大学 | Urban house refuse geologic landfill analogue experiment method and device |
| DE102009038017A1 (en) * | 2009-08-10 | 2011-02-24 | Bgd Boden- Und Grundwasserlabor Gmbh Dresden | Determination of microbial decomposition rates of first-order in fluid-saturated or fluid-partially saturated porous media of the soil- and groundwater areas, comprises flowing a water sample to be examined through successive reactors |
| CN104326558A (en) * | 2014-11-11 | 2015-02-04 | 南京大学 | Device for simulating in-situ river course sediment anaerobic ammoxidation process, and use method and application thereof |
| CN104777011A (en) * | 2015-03-26 | 2015-07-15 | 浙江大学 | Beach deposit collection and in situ ecology stimulation apparatus and method thereof |
| CN205353052U (en) * | 2016-01-25 | 2016-06-29 | 河海大学 | Mixed landfill post biochemical degradation test device of city domestic waste and mud |
| CN107358858A (en) * | 2017-07-18 | 2017-11-17 | 山西蓝焰煤层气集团有限责任公司 | Coal seam Biogenetic Gas Reservoir process simulation device and experimental method |
| CN109337809A (en) * | 2018-11-16 | 2019-02-15 | 曲阜师范大学 | A kind of early warning regulation method strengthened based on the unbalance microbial resistance of anaerobic digestion |
| CN109459341A (en) * | 2018-12-25 | 2019-03-12 | 西安科技大学 | A kind of experimental provision and method of Organic biological gas output |
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