CN208964946U - A kind of storage culture medium prevents the culture bottle of germ contamination - Google Patents

A kind of storage culture medium prevents the culture bottle of germ contamination Download PDF

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Publication number
CN208964946U
CN208964946U CN201821640880.9U CN201821640880U CN208964946U CN 208964946 U CN208964946 U CN 208964946U CN 201821640880 U CN201821640880 U CN 201821640880U CN 208964946 U CN208964946 U CN 208964946U
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China
Prior art keywords
bottle
culture
bottle cap
culture medium
minim pipette
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CN201821640880.9U
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Chinese (zh)
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柴海毅
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SHANGHAI NUODI BIOLOGICAL TECHNOLOGY Co Ltd
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SHANGHAI NUODI BIOLOGICAL TECHNOLOGY Co Ltd
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Abstract

The utility model discloses the culture bottles that a kind of storage culture medium prevents germ contamination, including bottle body and bottle cap, bottle cap is plugged in the top end opening of bottle body, liquid storage box is installed above bottle cap, perforative minim pipette also extends through bottle cap and imports in bottle body on liquid storage box, it is symmetrically installed that there are two hot types to stir flabellum below minim pipette, and also sets up below this section of minim pipette being located in liquid storage box there are two leak hole, the bottom end of bottle cap is equipped with temperature sensor;The culture bottle of the utility model can store culture medium by the liquid storage box being arranged, and culture medium can be added dropwise into bottle body by minim pipette, it can be to avoid bacterium infection culture, being provided with temperature sensor can be with temperature in sensing bottle, in order to stir temperature in flabellum heating bottle by hot type, suitable temperature is provided to culture, and pass through rotation minim pipette, hot type can be made to stir flabellum stir culture object, it is uniformly mixed the culture in bottle with newly added culture medium, avoids culture is viscous from being stained with bottle wall.

Description

A kind of storage culture medium prevents the culture bottle of germ contamination
Technical field
The utility model relates to a kind of culture bottle, in particular to a kind of storage culture medium prevents the culture bottle of germ contamination, Belong to culture bottle production technical field.
Background technique
The in vitro culture of cell and tissue has become the essential content of life studies and practice.The cell type of culture It is various, from virus to bacterium and fungi, it can suspend from human body cell to zooblast with plant cell, some cells and tissue It is grown under state, but quite a few mammalian cell needs surface adherent.Therefore, for providing cell injuring model environment Culture bottle, culture plate and culture dish in addition to good transparency and it is nontoxic, sterile other than, also need it is surface-modified processing make Can it is adherent, division and growth.Culture bottle can be divided into according to material it is glass and plastics, bottom shape have square, Rectangle, triangle etc. have 5 to 500ml selective according to bottle volume.It, can be in addition, surface is after special modification It allows cell preferably to carry out adhere-wall culture, more kinds of 25~175cm2 can be divided into again according to the adherent area of cell in this way.Culture bottle Bottleneck also there are many pattern, have straight neck, torticollis, angle neck, triangle, rectangle.
There are some disadvantages, culture bottles not to have the function of storage culture medium for current Tissue Culture Flask, adds culture medium When, it needs to open bottle cap, repeatedly opens bottle cap, easily lead to extraneous bacterium and enter in bottle body, pollute the culture in it, cause to train Support failure.
Utility model content
The utility model proposes the culture bottles that a kind of storage culture medium prevents germ contamination, solve and train in the prior art The function that bottle does not have storage culture medium is supported, when adding culture medium, needs to open bottle cap, repeatedly opens bottle cap, easily lead to the external world The problem of bacterium enters in bottle body, pollutes the culture in it, leads to culture failure.
In order to solve the above-mentioned technical problem, the utility model provides the following technical solution:
A kind of storage culture medium of the utility model prevents the culture bottle of germ contamination, including bottle body and bottle cap, the bottle body Top end opening in be plugged with bottle cap, liquid storage box is installed, perforative minim pipette is also worn on the liquid storage box above the bottle cap Cross bottle cap import bottle body in, be symmetrically installed below the minim pipette there are two hot type stir flabellum, the minim pipette it is upper Side is also equipped with valve, and the valve is located in the rotation slot being arranged on bottle cap, and this section of dropping liquid in liquid storage box It is also set up below pipe there are two leak hole, the bottom end of the bottle cap is equipped with temperature sensor, and the temperature sensor is by leading Line connects the temperature controller in bottle cap, and the temperature controller connects the temperature indicator installed on bottle cap, institute by conducting wire State the button cell power supply cooperated in bottle cap, and the push-button switch of the button cell, one upper side of bottle cap installation and two Hot type stirring flabellum is cascaded by conducting wire, forms series circuit.
As a kind of optimal technical scheme of the utility model, the hot type stirring flabellum is fan made of heater strip Paddle.
As a kind of optimal technical scheme of the utility model, the upper side connection of the liquid storage box with pipe close into Liquid pipe, and the junction of the inner bottom of the minim pipette and liquid storage box is provided with sealing ring.
As a kind of optimal technical scheme of the utility model, it is convex that the top of the minim pipette comes with circular rotation It rises.
As a kind of optimal technical scheme of the utility model, the bottle cap and plug is when on the bottleneck of bottle body, the rotation Slot position is less than the opening diameter length of bottle body in the overthe openings of bottle body, the overall width of two hot type stirring flabellums.
The utility model beneficial effect achieved is: the culture bottle of the utility model can be deposited by the liquid storage box of setting Culture medium is stored up, and culture medium can be added dropwise into bottle body by minim pipette, addition is convenient and healthy, can be to avoid bacterium infection culture Object, being provided with temperature sensor can be with temperature in sensing bottle, and temperature is shown on temperature indicator, in order to pass through Hot type stirs temperature in flabellum heating bottle, and suitable temperature is provided to culture, and by rotation minim pipette, can make to heat Type stirs flabellum stir culture object, is uniformly mixed the culture in bottle with newly added culture medium, avoids culture is viscous from being stained with bottle Wall, easy to use, in order to add culture, the danger of no bacterium infection is easy to use and promotes.
Detailed description of the invention
Attached drawing is used to provide a further understanding of the present invention, and constitutes part of specification, practical with this Novel embodiment is used to explain the utility model together, does not constitute limitations of the present invention.In the accompanying drawings:
Fig. 1 is the subjective structural schematic diagram of the utility model;
In figure: 1, bottle body;2, bottle cap;3, liquid storage box;4, inlet tube;5, minim pipette;6, hot type stirs flabellum;7, it leaks Hole;8, valve;9, rotation slot;10, pivot protrusion;11, temperature sensor;12, temperature controller;13, temperature indicator;14, Button cell;15, push-button switch.
Specific embodiment
It is illustrated below in conjunction with preferred embodiment of the attached drawing to the utility model, it should be understood that described herein excellent It selects embodiment to be only used for describing and explaining the present invention, is not used to limit the utility model.
Embodiment 1
As shown in Figure 1, the utility model, which provides a kind of storage culture medium, prevents the culture bottle of germ contamination, including bottle body 1 With bottle cap 2, bottle cap 2 is plugged in the top end opening of bottle body 1, the top of bottle cap 2 is equipped with liquid storage box 3, perforative drop on liquid storage box 3 Liquid pipe 5 also extend through bottle cap 2 import bottle body 1 in, the lower section of minim pipette 5 be symmetrically installed there are two hot type stir flabellum 6, minim pipette 5 top is also equipped with valve 8, and valve 8 is located in the rotation slot 9 being arranged on bottle cap 2, and this section drop being located in liquid storage box 3 The lower section of liquid pipe 5 is also set up there are two leak hole 7, and the bottom end of bottle cap 2 is equipped with temperature sensor 11, and temperature sensor 12 is by leading Line connects the temperature controller 13 in bottle cap 2, and temperature controller 13 connects the temperature indicator installed on bottle cap 2 by conducting wire 14, the interior button cell 14 cooperated of bottle cap 2 is powered, and 15 and of push-button switch of button cell 14,2 one upper side of bottle cap installation Two hot type stirring flabellums 6 are cascaded by conducting wire, form series circuit.
It is fan paddle made of heater strip that hot type, which stirs flabellum 6, can be with heating power, in order to warm in heating bottle Degree.
The upper side connection of liquid storage box 3 has the inlet tube 4 of pipe close, in order to supplement the culture medium in liquid storage box 3, and The junction of the inner bottom of minim pipette 5 and liquid storage box 3 is provided with sealing ring, and the culture medium in liquid storage box 3 is avoided to overflow.
The top of minim pipette 5 comes with circular pivot protrusion 7, in order to rotate minim pipette 5, stirs hot type thereon Mix 6 stir culture object of flabellum.
Bottle cap 2 is filled in when on the bottleneck of bottle body 1, and rotation slot 9 is located at the overthe openings of bottle body 1, and the opening of bottle body 1 is avoided to hinder Hindering the rotation of the valve 8 on minim pipette 5, the overall width of two stirring flabellums 6 is less than the opening diameter length of bottle body 1, in order to Export minim pipette 5.
Specifically, the liquid storage box 3 of setting can import storage culture medium by inlet tube 4, and valve 8 can be opened, made Culture medium in liquid storage box 3 is imported in minim pipette 5 by leak hole 7, and culture medium, addition side is added dropwise into bottle body 1 by minim pipette 5 Just hygienic, can occur to avoid opening the case where 2 bring bacterium infection culture of bottle cap, button cell 14 is powered, and is provided with Temperature sensor 11 can be with temperature in sensing bottle, and sends temperature to temperature controller 12, aobvious in temperature after being handled by it Show and shown on device 13, in order to staff's button press formula switch 15, button cell 14 and hot type is made to stir flabellum 6 Between circuit conducting, temperature in 6 heating bottle of flabellum is stirred by hot type, gives culture to provide suitable temperature, and pass through Minim pipette 5 is rotated, it is made to do reciprocating rotation in rotation slot 9, hot type can be made to stir 6 stir culture object of flabellum, made in bottle Culture be uniformly mixed with newly added culture medium, avoid culture is viscous from being stained with bottle wall.
The culture bottle of the utility model can store culture medium by the liquid storage box 3 of setting, and can by minim pipette 5 to Culture medium is added dropwise in bottle body 1, add it is convenient and healthy, can be to avoid bacterium infection culture, being provided with temperature sensor 11 can be with Temperature in sensing bottle, and temperature is shown on temperature indicator 13, in order to stir 6 heating bottle of flabellum by hot type Interior temperature provides suitable temperature to culture, and by rotation minim pipette 5, hot type can be made to stir 6 stir culture of flabellum Object is uniformly mixed the culture in bottle with newly added culture medium, avoids culture is viscous from being stained with bottle wall, easy to use, in order to Culture is added, the danger of no bacterium infection is easy to use and promotes.
Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention, it is not limited to this Utility model, although the utility model is described in detail with reference to the foregoing embodiments, for those skilled in the art For, it is still possible to modify the technical solutions described in the foregoing embodiments, or to part of technical characteristic It is equivalently replaced.Within the spirit and principle of the utility model, any modification, equivalent replacement, improvement and so on, It should be included within the scope of protection of this utility model.

Claims (5)

1. a kind of storage culture medium prevents the culture bottle of germ contamination, including bottle body (1) and bottle cap (2), which is characterized in that described It is plugged in the top end opening of bottle body (1) bottle cap (2), is equipped with liquid storage box (3) above the bottle cap (2), the liquid storage box (3) Upper perforative minim pipette (5) also extends through bottle cap (2) and imports in bottle body (1), be symmetrically installed below the minim pipette (5) there are two Hot type stirs flabellum (6), is also equipped with valve (8) above the minim pipette (5), the valve (8) is located on bottle cap (2) Leak hole there are two being also set up in the rotation slot (9) of setting, and below this section of minim pipette (5) positioned in liquid storage box (3) (7), the bottom end of the bottle cap (2) is equipped with temperature sensor (11), and the temperature sensor (11) connects bottle cap by conducting wire (2) temperature controller (12) in, the temperature controller (12) connect the temperature indicator installed on bottle cap (2) by conducting wire (13), button cell (14) power supply cooperated in the bottle cap (2), and the button cell (14), (2) one upper side of bottle cap are pacified The push-button switch (15) of dress and two hot type stirrings flabellum (6) are cascaded by conducting wire, form series circuit.
2. the culture bottle that a kind of storage culture medium according to claim 1 prevents germ contamination, which is characterized in that described to add It is fan paddle made of heater strip that heat type, which stirs flabellum (6),.
3. the culture bottle that a kind of storage culture medium according to claim 1 prevents germ contamination, which is characterized in that the storage The upper side connection of liquid box (3) has the inlet tube (4) of pipe close, and the inner bottom of the minim pipette (5) and liquid storage box (3) Junction be provided with sealing ring.
4. the culture bottle that a kind of storage culture medium according to claim 3 prevents germ contamination, which is characterized in that the drop The top of liquid pipe (5) comes with circular pivot protrusion (10).
5. the culture bottle that a kind of storage culture medium according to claim 1 prevents germ contamination, which is characterized in that the bottle (2) plug is covered when on the bottleneck of bottle body (1), the rotation slot (9) is located at the overthe openings of bottle body (1), two hot types The overall width for stirring flabellum (6) is less than the opening diameter length of bottle body (1).
CN201821640880.9U 2018-10-10 2018-10-10 A kind of storage culture medium prevents the culture bottle of germ contamination Active CN208964946U (en)

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CN201821640880.9U CN208964946U (en) 2018-10-10 2018-10-10 A kind of storage culture medium prevents the culture bottle of germ contamination

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Application Number Priority Date Filing Date Title
CN201821640880.9U CN208964946U (en) 2018-10-10 2018-10-10 A kind of storage culture medium prevents the culture bottle of germ contamination

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CN208964946U true CN208964946U (en) 2019-06-11

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112538427A (en) * 2020-11-16 2021-03-23 中国人民解放军陆军军医大学第二附属医院 Intestinal lymphocyte culture apparatus
CN113621500A (en) * 2021-08-17 2021-11-09 翁岳衡 Mechanical structure and operation method of online non-contact type OD value detection and automatic induction device

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112538427A (en) * 2020-11-16 2021-03-23 中国人民解放军陆军军医大学第二附属医院 Intestinal lymphocyte culture apparatus
CN113621500A (en) * 2021-08-17 2021-11-09 翁岳衡 Mechanical structure and operation method of online non-contact type OD value detection and automatic induction device

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