CN201158659Y - Semi-automatic adjustable quantitative liquid filler - Google Patents
Semi-automatic adjustable quantitative liquid filler Download PDFInfo
- Publication number
- CN201158659Y CN201158659Y CNU2007203102040U CN200720310204U CN201158659Y CN 201158659 Y CN201158659 Y CN 201158659Y CN U2007203102040 U CNU2007203102040 U CN U2007203102040U CN 200720310204 U CN200720310204 U CN 200720310204U CN 201158659 Y CN201158659 Y CN 201158659Y
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- quantitative liquid
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Abstract
The utility model discloses a semiautomatic and adjustable quantitative liquid filler used for cytobiology research. The semiautomatic and adjustable quantitative liquid filler comprises a glass tube triplet, an air-flow controller and a reagent bottle. The structure of the semiautomatic and adjustable quantitative liquid charger is specially designed; a vacuum pump is introduced; the air-flow controller is connected with the vacuum pump; the quantitative purpose can be realized through the piston inner tube of a turning bolt type measuring cylinder; and the suction and the discharge of liquid can be controlled through adjusting the open and the close of a liquid filling switch control valve. In the cell biology researches such as cell apoptosis, cell conjugation and cell transduction, and especially in the processes of massively preparing cells in vitro, the semiautomatic and adjustable quantitative liquid filler can greatly improve working efficiency, save working hour and decrease contamination possibility.
Description
Technical field
The utility model relates to a kind of liquid fillers, specifically a kind of semi-automatic adjustable quantitative liquid fillers that is used for RESEARCH ON CELL-BIOLOGY.
Background technology
Cell cultivation process, after the discovery cell, need to change liquid every three days, whole process relates to plurality of reagents and nutrient solution, and often a kind of reagent or nutrient solution need to add repeatedly repeatedly, complex operation not only, and draw the pollution that reagent or nutrient solution are easy to cause reagent or nutrient solution repeatedly, touch other article as transfer pipet in the operating process and will cause pollution.In case nutrient solution is contaminated in the operating process, will make that institute's cultured cells is also contaminated, whole simultaneously flask culture liquid all can not continue to use, and causes great waste economically, especially to the reagent of some expensive prices, as cytokine, superfine foetal calf serum etc.
At present, be used for the liquid-transfering gun and the liquid fillers of cell cultures, though in structure certain improvement is arranged, simplified operation to a certain extent, also have following problem: it is little 1, to add the amount of liquid variable range; 2, can not realize that mass cell is cultivated or the repeatable operation need once strengthen quantity of fluid the time; 3, liquid-transfering gun belongs to precision equipment, and is difficult to its sterilization, gets repeatedly in the liquid process to pollute easily; 4, get liquid with liquid-transfering gun operator's technical ability is had relatively high expectations, increased work difficulty; 5, need frequently change clean transfer pipet and washing and sterilizing difficulty, increase workload, waste research funding.
In sum, at present stage cell cultures work, RESEARCH ON CELL-BIOLOGY, as apoptosis, cytogamy, cell transduction, particularly during external a large amount of preparation cell such as production of vaccine, be badly in need of a kind of can reduce or avoid reagent contamination, easy and simple to handle, the application of sample amount is accurate, cheap, the liquid fillers of adjustable quantitative.
Summary of the invention
The purpose of this utility model is to remedy the deficiencies in the prior art and a kind of RESEARCH ON CELL-BIOLOGY that is used for is provided, carry out external a large amount of preparation cell and the semi-automatic adjustable quantitative liquid fillers that designs as apoptosis, cytogamy, cell transduction, particularly production of vaccine etc.
For achieving the above object, the utility model adopts following technical scheme:
A kind of RESEARCH ON CELL-BIOLOGY that is used for, as the semi-automatic adjustable quantitative liquid fillers of external a large amount of preparation cells such as apoptosis, cytogamy, cell transduction, particularly production of vaccine, it comprises grass tube triplet, air flow controller, reagent bottle.
Above-mentioned a kind of semi-automatic adjustable quantitative liquid fillers; Wherein, grass tube triplet and bolt type graduated cylinder fuse, and the grass tube lower end is goed deep in the liquid, above flat part reclinate slightly drain pipe is arranged, the top is air-breathing and deflated U type pipe.For convenience of operator's observed reading, the bolt type graduated cylinder adopts the translucent glass of oyster white to make.
Above-mentioned a kind of semi-automatic adjustable quantitative liquid fillers that is used for RESEARCH ON CELL-BIOLOGY; Wherein, air-breathing and deflated U type pipe coupling vacuum pump is in order to imbibition and discharge opeing.
Above-mentioned a kind of semi-automatic adjustable quantitative liquid fillers that is used for RESEARCH ON CELL-BIOLOGY; Wherein, the inner core of bolt type graduated cylinder and urceolus all are carved with the screw thread that matches, by the rotation of inner core in urceolus to reach quantitative purpose.
Advantage of the present utility model and benefit: semi-automatic adjustable quantitative liquid fillers described in the utility model is quantitatively accurate, can not cause waste again when keeping cell normal growth aequum.The variable range of the amount of liquid of getting is big, when inhaling big quantity of fluid without repeatable operation, thereby shorten working hours, increase work efficiency.Vacuum pump is connected with the liquid feeding switch, by the suction and the discharge of liquid feeding on-off control liquid, thereby alleviates staff's burden.
Below in conjunction with accompanying drawing and preferred forms the utility model being described further, so that the public has whole and understanding fully to summary of the invention, and is not the qualification to the utility model protection domain.Aforementioned part fully discloses the protection domain that the utility model can be implemented, and therefore allly any well known in the artly is equal to replacement according to what the utility model disclosure was carried out, all belongs to infringement of the present utility model.
Description of drawings
Fig. 1 is the utility model preferred embodiment 1 synoptic diagram.
Fig. 2 is the utility model grass tube triplet synoptic diagram.
Fig. 3 is the utility model air flow controller synoptic diagram.
Fig. 4 is the utility model reagent bottle synoptic diagram.
Embodiment
A kind of RESEARCH ON CELL-BIOLOGY that is used for, as the semi-automatic adjustable quantitative liquid fillers of external a large amount of preparation cells such as apoptosis, cytogamy, cell transduction, particularly production of vaccine, it comprises air flow controller 1, grass tube triplet 2, reagent bottle 3; Air flow controller 1 comprises U type pipe 11, air blowing rubber hose 12, the rubber hose of bleeding 13, filter 14, liquid feeding switch 15, rubber hose 16; Grass tube triplet 2 comprises ventpipe 21, bolt type graduated cylinder inner core 22, bolt type graduated cylinder urceolus 23, liquid discharge pipe 24, drain pipe 25, pipette 26, rubber hose 27; Described bolt type graduated cylinder inner core 22 is provided with screw thread 221, front end has piston 222; Described bolt type graduated cylinder urceolus 23 is provided with scale 231, and the inner core of bolt type graduated cylinder and urceolus all are carved with the screw thread that matches, by the rotation of inner core in urceolus to reach quantitative purpose.Ventpipe 21 is positioned at the upper end of grass tube triplet 2, and the middle part is shrunk to one and limits the quantity of mouthfuls 211, and these mouthful 211 bottoms of limiting the quantity of are provided with a semiclosed stop section 212, limit the quantity of mouthfuls 211 and stop section 212 between be provided with buoy control valve 213; Pipette 26 is positioned at the lower end of grass tube triplet 2, and the middle and lower part is shrunk to one and limits the quantity of mouthfuls 261, and these mouthful 261 tops of limiting the quantity of are provided with a semiclosed stop section 262, limit the quantity of mouthfuls 261 and stop section 262 between be provided with gravity control valve 263; Liquid discharge pipe 24 is positioned at the left end of grass tube triplet 2, and the middle part is shrunk to one and limits the quantity of mouthfuls 241, and these mouthful 241 tops of limiting the quantity of are provided with a semiclosed stop section 242, limit the quantity of mouthfuls 241 and stop section 242 between be provided with gravity control valve 243; Liquid discharge pipe 24 is linked to each other by rubber hose 27 with drain pipe 25.The sealing cover 31 of described reagent bottle 3 is provided with liquid filling hole 32.
During use, adding small amount of liquid can directly pour into from liquid filling hole 32; Can liquid filling hole 32 be linked to each other with the pressure liquid discharge device by rubber hose when adding big quantity of fluid liquid is changed in the reagent bottle 3.U type pipe 11 is linked to each other with vacuum pump with the rubber hose 13 of bleeding by air blowing rubber hose 12, and swivel bolt formula inner core is to the predetermined graduated place; Open vacuum pump, the gas in the graduated cylinder is drawn out of, and air pressure reduces, and the gravity control valve 243 of liquid discharge pipe 24 moves down blocks liquid outlet and the gravity control valve 263 of pipette is washed open, and liquid enters the bolt type graduated cylinder.Along with liquid increases; buoy control valve 213 floats gradually; buoy is just in time blocked the oral area of U type pipe 11 when reaching predetermined volumes; open liquid feeding switch 15 this moment; the rubber hose of bleeding 13 is exhaust; buoy control valve 213 is blowed to the below, gravity control valve 263 moves down and does not allow liquid return, thereby the valve of washing liquid outlet simultaneously open is discharged liquid.A hand control liquid feeding switch 15, the another hand is taken culturing bottle, and the closure by control liquid feeding switch 15 can will join in the culturing bottle liquid continuously.
Claims (5)
1, a kind of semi-automatic adjustable quantitative liquid fillers that is used for RESEARCH ON CELL-BIOLOGY, it is characterized in that: it comprises grass tube triplet, air flow controller, reagent bottle; The grass tube triplet comprises ventpipe, drain pipe, liquid-inlet pipe, and they link to each other with upper end, left end, the lower end of bolt type graduated cylinder respectively; The middle part of ventpipe, pipette, liquid discharge pipe all is shrunk to the mouth of limiting the quantity of, and mouthful top of limiting the quantity of is provided with a semiclosed stop section, limit the quantity of mouthful and the stop section between be provided with a valve body; Air flow controller comprises U type pipe, rubber catheter, filter, liquid feeding switch.
2, a kind of semi-automatic adjustable quantitative liquid fillers that is used for RESEARCH ON CELL-BIOLOGY according to claim 1, it is characterized in that: described air flow controller links to each other with vacuum pump.
3, a kind of semi-automatic adjustable quantitative liquid fillers that is used for RESEARCH ON CELL-BIOLOGY according to claim 1, it is characterized in that: described grass tube triplet has three spherical control valves, is the buoy control valve in the ventpipe, is made by buoyancy material; Two other force control valve of attaching most importance to is made by glass.
4, a kind of semi-automatic adjustable quantitative liquid fillers that is used for RESEARCH ON CELL-BIOLOGY according to claim 1, it is characterized in that: described reagent bottle covers and is provided with a liquid filling hole.
5, a kind of semi-automatic adjustable quantitative liquid fillers that is used for RESEARCH ON CELL-BIOLOGY according to claim 1, it is characterized in that: U type pipe links to each other with the grass tube triplet with rubber hose with drain pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007203102040U CN201158659Y (en) | 2007-12-05 | 2007-12-05 | Semi-automatic adjustable quantitative liquid filler |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007203102040U CN201158659Y (en) | 2007-12-05 | 2007-12-05 | Semi-automatic adjustable quantitative liquid filler |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201158659Y true CN201158659Y (en) | 2008-12-03 |
Family
ID=40109252
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2007203102040U Expired - Fee Related CN201158659Y (en) | 2007-12-05 | 2007-12-05 | Semi-automatic adjustable quantitative liquid filler |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201158659Y (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106640776A (en) * | 2017-02-15 | 2017-05-10 | 江苏源远检测科技有限公司 | Automatic U-shaped pipe liquid injection device |
| CN107907644A (en) * | 2017-11-24 | 2018-04-13 | 江苏海德环境科技有限公司 | With anti-oxidation and exclusion air interference COD water samples metering device and method |
| CN108816302A (en) * | 2018-08-13 | 2018-11-16 | 泉州医学高等专科学校 | A kind of quantitative drainage head and drainage method suitable for reagent bottle |
| CN110540159A (en) * | 2018-10-30 | 2019-12-06 | 满金坝(深圳)科技有限公司 | automatic liquid pumping machine |
| CN112608846A (en) * | 2021-01-15 | 2021-04-06 | 吉林大学中日联谊医院 | Multifunctional cell culture device for improving premature ovarian failure stem cell exosomes |
| CN112777160A (en) * | 2021-01-26 | 2021-05-11 | 宁夏嘉铂睿环保科技集团有限公司 | General diluent holds device convenient to ration is unloaded |
| CN113856783A (en) * | 2021-12-02 | 2021-12-31 | 潍坊市检验检测中心 | Food inspection ultrasonic cavitation liquid-transfering device |
-
2007
- 2007-12-05 CN CNU2007203102040U patent/CN201158659Y/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106640776A (en) * | 2017-02-15 | 2017-05-10 | 江苏源远检测科技有限公司 | Automatic U-shaped pipe liquid injection device |
| CN107907644A (en) * | 2017-11-24 | 2018-04-13 | 江苏海德环境科技有限公司 | With anti-oxidation and exclusion air interference COD water samples metering device and method |
| CN107907644B (en) * | 2017-11-24 | 2024-03-22 | 江苏海德环境科技有限公司 | COD water sample metering device and method with functions of preventing oxidization and eliminating air interference |
| CN108816302A (en) * | 2018-08-13 | 2018-11-16 | 泉州医学高等专科学校 | A kind of quantitative drainage head and drainage method suitable for reagent bottle |
| CN110540159A (en) * | 2018-10-30 | 2019-12-06 | 满金坝(深圳)科技有限公司 | automatic liquid pumping machine |
| CN112608846A (en) * | 2021-01-15 | 2021-04-06 | 吉林大学中日联谊医院 | Multifunctional cell culture device for improving premature ovarian failure stem cell exosomes |
| CN112777160A (en) * | 2021-01-26 | 2021-05-11 | 宁夏嘉铂睿环保科技集团有限公司 | General diluent holds device convenient to ration is unloaded |
| CN113856783A (en) * | 2021-12-02 | 2021-12-31 | 潍坊市检验检测中心 | Food inspection ultrasonic cavitation liquid-transfering device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20081203 Termination date: 20141205 |
|
| EXPY | Termination of patent right or utility model |