CN200992554Y - Continuous filling device for cell culture - Google Patents
Continuous filling device for cell culture Download PDFInfo
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- CN200992554Y CN200992554Y CN 200720000621 CN200720000621U CN200992554Y CN 200992554 Y CN200992554 Y CN 200992554Y CN 200720000621 CN200720000621 CN 200720000621 CN 200720000621 U CN200720000621 U CN 200720000621U CN 200992554 Y CN200992554 Y CN 200992554Y
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- liquid
- cell cultures
- adding device
- piston
- scale
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Abstract
The utility model discloses a continuous fluid filling device for cell culture, which consists of a fluid filling machine and a fluid storage bottle. The fluid filling machine consists of a plunger, a plunger bushing, a sealing lid and an outflow pipe. The plunger is arranged inside the plunger bushing. The sealing lid with scaleplate is arranged in the plunger bushing. The end of the plunger bushing is equipped with a pipette. The lower side wall of the plunger bushing has a discharging pipe. The pipette and the discharging pipe is communicated with the plunger bushing. The discharging pipe transfixes the sealing lid and connects to the outflow pipe. The middle of the pipette contracts to a rationed gate, so does the discharging pipe. A semienclosed arresting device is arranged on the upper rationed gate. A valve is arranged between the rationed gate and the arresting device. The storage bottle connects to the sealing lid spirally. The utility model is capable of reducing or avoiding contamination in operation, simplifying the operation, lowering the difficulty in operation and improving the precision of liquid filling. The device is low in cost and convenient in sterilization.
Description
Technical field
The utility model relates to a kind of liquid-adding device, specifically a kind of continuous liquid-adding device that is used for cell cultures.
Background technology
At present, the method for existing cell cultures comprises adherent culture, the cultivation of Epstein-Barr virus transformation technology, the cultivation of collagenase digesting technology etc.Because cell cultures has a plurality of stages, so in culturing process, generally will relate to plurality of reagents and nutrient solution, and often a kind of reagent or nutrient solution need to add repeatedly repeatedly, complex operation not only, and draw the pollution that reagent or nutrient solution are easy to cause reagent or nutrient solution repeatedly, when screwing the reagent bottle cap repeatedly and pipettor when repeatedly stretching into reagent bottle as operator.The reagent of some kind that cell cultures is used and nutrient solution are very expensive, as superfine foetal calf serum etc., and when in cultivating plate hole, adding substratum since each application of sample dosage seldom, big bottled nutrient solution is difficult to use up in a short time, so attention of in the use of nutrient solution, more being careful, in case nutrient solution is contaminated in operating process, whole flask culture liquid all can not continue to use, and has caused great waste economically.Existing liquid-transfering gun that is used for cell cultures and transfer pipet such as the patent No. are the pipettor of ZL200420020854.8, though done certain improvement in the pipettor structure, simplified operation to a certain extent, but also there is following problems, 1, cooperate just and can finish as two people of whole liquid feeding sampling operation process need; 2, because the each application of sample of liquid-transfering gun all needs to change clean transfer pipet, in the process of cell cultures, not only need a large amount of transfer pipets, and the washing and sterilizing difficulty, this has not only increased workload but also has wasted unnecessary research funding for the scientific research personnel; 3, because liquid-transfering gun belongs to precision equipment, be difficult to it is sterilized, get easier polluting in the process of liquid repeatedly at liquid-transfering gun; 4, get liquid with liquid-transfering gun operator's technical ability is had relatively high expectations, increased work difficulty.
In sum, present stage is badly in need of a kind ofly can reducing or avoid reagent contamination, easy and simple to handle, liquid-adding device that the application of sample beam is accurate, cheap at cell cultures work.
Summary of the invention
In order to remedy the deficiencies in the prior art, the inventor is through experiment repeatedly, finally proposed a kind ofly can effectively avoid reagent contamination, the continuous liquid-adding device that is used for cell cultures easy and simple to handle.
To achieve these goals, the utility model adopts following technical scheme:
A kind of continuous liquid-adding device that is used for cell cultures, it comprises liquid fillers and liquid storage bottle; Liquid fillers comprises piston, piston sleeve, sealing cover and drain pipe; Described piston is positioned at piston sleeve; Be arranged with sealing cover on the piston sleeve, sealing covers and is provided with scale; This piston sleeve end has extended pipette, and also is provided with liquid discharge pipe on the sidewall of its underpart, and described pipette and liquid discharge pipe communicate with piston sleeve; This liquid discharge pipe links to each other through sealing cover and with drain pipe; The middle part of described pipette and liquid discharge pipe all is shrunk to the mouth of limiting the quantity of, and this mouthful top of limiting the quantity of is equipped with a semiclosed stop section, limit the quantity of mouthful and the stop section between be provided with a valve body; Described liquid storage bottle and sealing cover spin.
The above-mentioned continuous liquid-adding device that is used for cell cultures, wherein, described sealing cover is provided with a liquid filling hole.
The above-mentioned continuous liquid-adding device that is used for cell cultures, wherein, described liquid discharge pipe links to each other with drain pipe by rubber hose.
The above-mentioned continuous liquid-adding device that is used for cell cultures, wherein, described scale is a non-cylindrical configuration, which is provided with scale, and this graduated range is 0.1ml～10ml, and range is by the diameter and the volume controlled of piston sleeve.
The above-mentioned continuous liquid-adding device that is used for cell cultures, wherein, described liquid storage bottle wall is provided with horizontal corrugated projection or described liquid storage bottle bottle wall and is provided with fin, is easy to hold in order to increase frictional force.
The above-mentioned continuous liquid-adding device that is used for cell cultures wherein, comprises that also a base can be placed in the liquid storage bottle bottom, to increase the height of liquid storage bottle height in order to equipment such as adaptation spirit lamps, has also increased the stationarity of liquid storage bottle simultaneously.
The above-mentioned continuous liquid-adding device that is used for cell cultures, wherein, described piston is provided with the mounting block of a band deep-slotted chip breaker, and this mounting block is limited to piston on the scale by deep-slotted chip breaker, makes the piston can not left rotation and right rotation, can only move up and down.
The above-mentioned continuous liquid-adding device that is used for cell cultures, wherein, be arranged with on the described scale one with the stop ring moving up and down of its coupling, described stop ring is provided with a bolt and can be fixed on the scale.
The above-mentioned continuous liquid-adding device that is used for cell cultures, wherein, described mounting block is positioned at the stop ring below.
Sealing cover of the present utility model, be with graduated scale and piston etc. can adopt resistant to elevated temperatures rigid plastics material, piston sleeve, pipette, liquid discharge pipe, valve body and drain pipe etc. can adopt glass material.
Principle of work of the present utility model: when piston upwards spurs, form vacuum in the piston sleeve, this moment, liquid discharge pipe was blocked its throttle orifice downwards owing to its valve body of suction function, and whole liquid discharge pipe is in closure state; Because the barometric point effect makes and moves on the valve body in the pipette simultaneously that liquid enters piston sleeve by pipette.When piston when pressing down, the piston sleeve internal pressure is greater than normal atmosphere, the valve body in pipette effect is downwards blocked its throttle orifice, at this moment liquid enters liquid discharge pipe, the effect of being under pressure of valve body in the liquid discharge pipe moves up, and opens throttle orifice, and liquid flows out from drain pipe through liquid discharge pipe.
Advantage of the present utility model and benefit: liquid fillers described in the utility model sealing is tight, effectively reduces and has avoided the pollution in the operating process; On sealing cover, be provided with a fluid injection sky, can under the situation that need not open sealing cover, reagent or nutrient solution be added in the liquid storage bottle, further avoid polluting; Whole liquid feeding process can have been simplified operation steps by single finishing; Liquid volume added can be selected voluntarily according to range, can change operations such as liquid fast and accurately, has improved working efficiency; Design for the piston upper fixing element makes piston be easier to slide up and down, and has reduced operation easier; The design of stop ring moves piston to add the high scale precision, to have increased the precision of liquid feeding on the scale in required range ability; Described liquid discharge pipe links to each other with drain pipe by rubber hose, has played good sealing function, and can change different drain pipes according to the test needs; The utility model is simple in structure, material is rigid plastics and glass, and is not only with low cost, and sterilization is convenient.
Below in conjunction with accompanying drawing and preferred forms the utility model being described further, so that the public has whole and understanding fully to summary of the invention, and is not the qualification to the utility model protection domain.Aforementioned part fully discloses the protection domain that the utility model can be implemented, and therefore allly any well known in the artly is equal to replacement according to what the utility model disclosure was carried out, all belongs to infringement of the present utility model.
Description of drawings
Fig. 1 is the utility model preferred embodiment 1 synoptic diagram;
Fig. 2 is the utility model preferred embodiment 1 liquid fillers (on carry state) synoptic diagram;
Fig. 3 is the utility model preferred embodiment 2 synoptic diagram;
Fig. 4 is the utility model preferred embodiment 2 liquid fillerss (pressed status) synoptic diagram;
Fig. 5 overlooks enlarged view for the utility model;
Fig. 6 is the utility model piston upper fixing element synoptic diagram;
Fig. 7 is the utility model preferred embodiment 3 synoptic diagram;
Fig. 8 is the utility model preferred embodiment 4 synoptic diagram.
Embodiment
As Fig. 1, Fig. 2 (carrying state on the piston), a kind of continuous liquid-adding device that is used for cell cultures, it comprises liquid fillers 1 and liquid storage bottle 2; Liquid fillers 1 comprises piston 11, piston sleeve 12, sealing cover 13 and drain pipe 14; Described piston 11 is positioned at piston sleeve 12, and can move up and down; Be arranged with sealing cover 13 on the piston sleeve 12, sealing lid 13 is provided with scale 131; These piston sleeve 12 ends have extended pipette 15, and also are provided with liquid discharge pipe 16 on the sidewall of its underpart, and pipette 15 and liquid discharge pipe 16 all communicate with piston sleeve 12; This liquid discharge pipe 16 links to each other through sealing cover 13 and with drain pipe 14; Described pipette 15 middle parts are shrunk to one and limit the quantity of mouthfuls 151, and these mouthful 151 tops of limiting the quantity of are provided with a semiclosed stop section 152, limit the quantity of mouthfuls 151 and stop section 152 between be provided with a valve body 153; Described liquid discharge pipe 16 middle parts are shrunk to one and limit the quantity of mouthfuls 161, and these mouthful 161 tops of limiting the quantity of are provided with a semiclosed stop section 162, limit the quantity of mouthfuls 161 and stop section 162 between be provided with a valve body 163; Described liquid storage bottle 2 spins with sealing cover 13.
Shown in Fig. 3,4,5, on the architecture basics of embodiment 1, sealing cover 13 is provided with a liquid filling hole 132; Liquid discharge pipe 16 links to each other with drain pipe 14 by rubber hose 17; Scale 131 is a non-cylindrical configuration, which is provided with scale; As shown in Figure 6, piston 11 is provided with the mounting block 111 of a band deep-slotted chip breaker, and this mounting block 111 is limited to piston 11 on the scale 131 by deep-slotted chip breaker can not left rotation and right rotation; Be arranged with on the scale 131 one with the stop ring moving up and down 133 of its coupling; Stop ring 133 is provided with a bolt 134, when bolt 134 can move up and down on scale 131 at stop ring 133 under the relaxed state, 134 stop ring 133 can be fixed on the scale 131 when tighting a bolt; Described mounting block 111 is positioned at stop ring 133 belows; Described liquid storage bottle 2 walls are provided with horizontal corrugated projection 21.
As shown in Figure 7, add a base 3 in addition on the architecture basics of embodiment 2, base 3 is placed in liquid storage bottle 2 bottoms.
Embodiment 4
As shown in Figure 8, the bottle wall of liquid storage bottle 2 is provided with vertical fin, and other structures are with embodiment 2.
Principle of work of the present utility model: at first, stop ring 133 lower edges are set in the scale marks of the required annex solution scale of construction, tight a bolt 134 with fixed stop stop ring 133 on scale 131, piston 11 moves up to stop ring 133 promptly by block; When piston 11 upwards spurs, form vacuum in the piston sleeve 12, this moment, liquid discharge pipe 16 was blocked its throttle orifice 161 downwards owing to its valve body 163 of suction function, and whole liquid discharge pipe 16 is in closure state; Because the barometric point effect makes and moves on the valve body 153 in the pipette 15 simultaneously that liquid enters in the piston sleeve 12 by pipette 15.When piston 11 when pressing down, piston sleeve 12 internal pressures are greater than normal atmosphere, its throttle orifice 151 is blocked in valve body 153 effect downwards in the pipette 15, at this moment liquid enters liquid discharge pipe 16, the effect of being under pressure of valve body 163 in the liquid discharge pipe 16 moves up, open throttle orifice 161, liquid flows out from drain pipe 14 through liquid discharge pipe 16.
Claims (10)
1, a kind of continuous liquid-adding device that is used for cell cultures is characterized in that it comprises liquid fillers and liquid storage bottle; Liquid fillers comprises piston, piston sleeve, sealing cover and drain pipe; Described piston is positioned at piston sleeve; Be arranged with sealing cover on the piston sleeve, sealing covers and is provided with scale; This piston sleeve end has extended pipette, and its underpart sidewall also is provided with liquid discharge pipe, and pipette and liquid discharge pipe communicate in piston sleeve; This liquid discharge pipe links to each other through sealing cover and with drain pipe; The middle part of described pipette and liquid discharge pipe all is shrunk to the mouth of limiting the quantity of, and respectively this mouthful top of limiting the quantity of is provided with a semiclosed stop section, limit the quantity of mouthful and the stop section between be provided with a valve body; Described liquid storage bottle and sealing cover spin.
2, the continuous liquid-adding device that is used for cell cultures according to claim 1 is characterized in that described sealing cover is provided with a liquid filling hole.
3, the continuous liquid-adding device that is used for cell cultures according to claim 1 is characterized in that described liquid discharge pipe links to each other with drain pipe by rubber hose.
4, the continuous liquid-adding device that is used for cell cultures according to claim 1 is characterized in that described scale is a non-cylindrical configuration, which is provided with scale.
5, the continuous liquid-adding device that is used for cell cultures according to claim 1 is characterized in that, described liquid storage bottle wall is provided with transverse wave shape projection or is provided with fin, in order to increase frictional force.
6, the continuous liquid-adding device that is used for cell cultures according to claim 1 is characterized in that, comprises that also a base can be placed in the liquid storage bottle bottom.
7, the continuous liquid-adding device that is used for cell cultures according to claim 1 is characterized in that, described piston is provided with the mounting block of a band deep-slotted chip breaker, and this mounting block is limited to piston by deep-slotted chip breaker can not left rotation and right rotation on the scale.
8, the continuous liquid-adding device that is used for cell cultures according to claim 7 is characterized in that, be arranged with on the described scale one with the stop ring moving up and down of its coupling.
9, the continuous liquid-adding device that is used for cell cultures according to claim 8 is characterized in that described stop ring is provided with a bolt and can be fixed on the scale.
10, the continuous liquid-adding device that is used for cell cultures according to claim 8 is characterized in that, described mounting block is positioned at the stop ring below.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200720000621 CN200992554Y (en) | 2006-12-08 | 2007-01-10 | Continuous filling device for cell culture |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200620167403 | 2006-12-08 | ||
| CN200620167403.6 | 2006-12-08 | ||
| CN 200720000621 CN200992554Y (en) | 2006-12-08 | 2007-01-10 | Continuous filling device for cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN200992554Y true CN200992554Y (en) | 2007-12-19 |
Family
ID=38945630
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200720000621 Expired - Fee Related CN200992554Y (en) | 2006-12-08 | 2007-01-10 | Continuous filling device for cell culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN200992554Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110257224A (en) * | 2019-07-26 | 2019-09-20 | 济南市疾病预防控制中心 | A kind of microculture instant culture medium additive storage device |
| CN113717852A (en) * | 2021-09-03 | 2021-11-30 | 重庆市盛佰昱科技有限公司 | Liquid suction method and liquid feeding method for biological tissue culture solution |
| CN114231413A (en) * | 2021-12-16 | 2022-03-25 | 潘烨 | A isolated culture equipment that is used for hematology branch of academic or vocational study marrow mesenchymal stem cell |
-
2007
- 2007-01-10 CN CN 200720000621 patent/CN200992554Y/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110257224A (en) * | 2019-07-26 | 2019-09-20 | 济南市疾病预防控制中心 | A kind of microculture instant culture medium additive storage device |
| CN113717852A (en) * | 2021-09-03 | 2021-11-30 | 重庆市盛佰昱科技有限公司 | Liquid suction method and liquid feeding method for biological tissue culture solution |
| CN114231413A (en) * | 2021-12-16 | 2022-03-25 | 潘烨 | A isolated culture equipment that is used for hematology branch of academic or vocational study marrow mesenchymal stem cell |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20071219 Termination date: 20150110 |
|
| EXPY | Termination of patent right or utility model |