Background technology
When cells in vitro was cultivated, the cell biological reactor was the key equipment of whole incubation, for cell provides a suitable growing environment, makes it fast breeding and forms required biological tissue's goods.At present; The cell biological reactor that uses in the biological industry has conventional cell biological reactor, disposable cell biological reactor etc.; The tail gas outlet side of these cell biological reactors all can be connected with exhaust gas processing device; Drain into the external world again after gas in the reactor handled, and the isolated cell bioreactor is inner contacts with external environment condition, prevents in the outside bacterium entering reactor.
These exhaust gas processing devices comprise air cleaner or alcohol breathing bag.Wherein air cleaner generally includes hollow housing, and the housing two ends are respectively equipped with air inlet and gas outlet, and the filter membrane that only allows gas to pass through is set between air inlet and gas outlet in the chamber of housing.The alcohol breathing bag then is to comprise a polybag; The top of this polybag is provided with gas discharge outlet; The bottom is provided with the gas access, is provided with at the place, gas access to be used for and cell biological reactor tube connector, fills the alcohol that accounts for half volume of plastic containers in the bag; In use; The gas access is communicated with the tail gas outlet side of cell biological reactor, and the tail gas of discharging in the cell biological reactor gets into the alcohol breathing bag through the gas access of alcohol breathing bag and handles through the absorption of alcohol, and the gas that is insoluble to alcohol is discharged from gas discharge outlet.
But because cell biological reactor internal gas pressure is much higher than the outside air pressure of reactor, and the nutrient solution in the reactor can produce foam when flowing, when the liquid level in the reactor rises and air-flow when excessive; The foam of nutrient solution or generation very easily flows out along the tail gas pipeline, because air cleaner only allows gas to pass through, when nutrient solution and foam outflow; Can stop up air cleaner or breathing bag, cause tail gas to discharge smoothly, cause the aerating system in the reactor to work; The biological respinse of reactor can't be carried out, can cause the failure of an experiment, cause damage; Moreover owing to sneak into nutrient solution in air cleaner or the breathing bag; Can let nutrient solution contact, infect extraneous bacterium, influence the completion of the biological respinse in the bioreactor with extraneous.
Summary of the invention
The purpose of the utility model aims to provide a kind of cell biological reactor tail gas treating apparatus; The foam that this device has solved nutrient solution or generation effectively very easily flows out air cleaner or breathing bag obstruction and the contaminated problem of nutrient solution that causes along the tail gas pipeline of cell biological reactor; Guarantee that the tail gas in the cell biological reactor discharges smoothly, guarantee that biological respinse carries out smoothly.
The purpose of the utility model realizes through following technical measures: a kind of cell biological reactor tail gas treating apparatus; It is characterized in that comprising an airtight tail gas hold up vessel; On described tail gas hold up vessel, be provided with gas outlet and the air inlet that is used for being communicated with, be connected with air cleaner in the gas outlet of said tail gas hold up vessel with the outlet of cell biological reactor.The cell biological reactor tail gas gets in the tail gas hold up vessel through air inlet pipe; In the tail gas hold up vessel, be able to buffering; Reduced the flow velocity of air-flow, made with the nutrient solution of tail gas outflow or the foam of generation to be able to separate in this space with gas, nutrient solution or foam rest in the container; Can not stop up air cleaner, gas then can be smoothly outside air cleaner drains into the tail gas hold up vessel.
Described tail gas hold up vessel mainly is made up of top body with opening and the top cover that is positioned on the opening, and described air inlet and gas outlet are separately positioned on the said top cover.
The utility model is provided with air inlet pipe on the air inlet of described tail gas hold up vessel; The mouth of pipe at said air inlet pipe two ends is respectively as inlet end and outlet side; This air inlet pipe is as the deep layer pipe of present embodiment; Its inlet end is connected with and is used for the jointing that is connected with the outlet of cell biological reactor, and the outlet side is then passed the gas outlet and extended the bottom in the described tail gas hold up vessel, and leaves the gap between the bottom surface of this outlet side and tail gas hold up vessel.Tail gas in the cell biological reactor is delivered to tail gas hold up vessel cavity bottom earlier through this air inlet pipe, upwards flows from the bottom again, goes out to flow out from the gas outlet.Even and the foam of nutrient solution or generation gets into the tail gas hold up vessel with tail gas along air inlet pipe; Tail gas then flows from bottom to top and after air cleaner filters, drains into tail gas hold up vessel outside; The foam of nutrient solution or generation is stayed in the tail gas hold up vessel, reaches the abundant separation of gas-liquid.
The utility model sets out the pipe of breathing hard at the place, gas outlet of described tail gas hold up vessel; Shallow-layer pipe as present embodiment; The mouth of pipe at these short tube two ends of giving vent to anger is respectively as inlet end and outlet side; The inlet end of short tube of giving vent to anger passes said gas outlet and extends the top in the tail gas hold up vessel, and said air cleaner is connected the outlet side of the short tube of giving vent to anger.
An improvement as the utility model: in said tail gas hold up vessel, absorbent solution is housed, the outlet side of said air inlet pipe is arranged in said absorbent solution, and absorbent solution is used for the rough estimate exhaust flow, absorb soluble gas and tail gas is carried out disinfection.
As the utility model preferred embodiment, the outlet side port of a little higher than said air inlet pipe of liquid level of described absorbent solution, promptly absorbent solution just there be not the outlet side port of air inlet pipe.
According to actual needs, described absorbent solution can adopt pure water or alcohol or thimerosal.
Compared with prior art, the utlity model has following beneficial effect:
(1)The utlity model has the tail gas hold up vessel; For the gas that gets in this container provides cushion space; Can reduce the flow velocity of air-flow, make with the nutrient solution of tail gas outflow or the foam of generation to be able to separate in this space with gas, nutrient solution or foam rest in the container; Can not stop up air cleaner, gas then can be smoothly outside air cleaner drains into the tail gas hold up vessel.
The outlet side of the air inlet pipe of the utility model is stretched in the chamber of tail gas hold up vessel near container bottom; And leave the gap between the bottom surface of this outlet side and tail gas hold up vessel, the tail gas in the cell biological reactor is delivered to tail gas hold up vessel cavity bottom earlier through this air inlet pipe, further reduces the flow velocity of gas; The nutrient solution that prolongs tail gas and flow out with tail gas or foam time of staying in the tail gas hold up vessel of generation; Gas then flows from bottom to top and discharges from the gas outlet, and the foam of nutrient solution or generation is stayed in the tail gas hold up vessel, reaches the abundant separation of gas-liquid; Avoid stopping up air cleaner; Guarantee that in the cell biological course of reaction, gas can both be discharged smoothly, guarantee carrying out smoothly of biological respinse.
The utility model is connected with the exhaust outlet of cell biological reactor through jointing; Liquid in flowing into the tail gas hold up vessel is too much; Have when take place stopping up air cleaner possible; The new gas emission device of in time crossing with aseptic process is changed through quick aseptic jointing, and is simple.
The specific embodiment
Cell biological reactor tail gas treating apparatus shown in Figure 1 is an embodiment of the utility model; Comprise that airtight tail gas is detained bottle 1; This tail gas is detained bottle 1 and comprises that the top has the body 11 and top cover 12 formations that are positioned on the opening of opening; Top cover 12 is provided with air inlet pipe 5 for being provided with the Liang Tonggai of air inlet and gas outlet at air inlet, and this air inlet pipe 5 is as the deep layer pipe of present embodiment.The mouth of pipe at air inlet pipe 5 two ends is respectively as inlet end and outlet side, and its inlet end is connected with jointing 6, and this jointing 6 is used for being connected with the gas outlet of cell biological reactor, is communicated with cell biological reactor and tail gas and is detained bottle 1.The outlet side of air inlet pipe 5 is passed the gas outlet and is extended described tail gas and be detained the bottom in the body 11 of bottle 1, and leaves the gap between the bottom surface of this outlet side and body 11.Set out the pipe 3 of breathing hard in the gas outlet of top cover 12, as the shallow-layer pipe of present embodiment.The mouth of pipe at these short tube 3 two ends of giving vent to anger is respectively as inlet end and outlet side, and its inlet end passes the gas outlet of top cover 12 and stretches in the tail gas delay bottle 1, and is positioned at the top that tail gas is detained bottle 1, and the outlet side connects air cleaner 4.In the tail gas delay bottle 1 absorbent solution is housed; Be used for the rough estimate exhaust flow, absorb soluble gas and tail gas is carried out disinfection; The outlet side port of a little higher than said air inlet pipe 5 of the liquid level of absorbent solution; Be the outlet side port that absorbent solution just there be not air inlet pipe 5, and absorbent solution adopt pure water or alcohol or thimerosal according to actual needs.
The gas outlet of cell biological reactor is connected with jointing 6 through tube connector; Be detained bottle 1 with tail gas and be communicated with, the tail gas in the cell biological reactor is delivered to tail gas earlier through air inlet pipe 5 and is detained bottle 1 cavity bottom, and tail gas is detained bottle 1 at tail gas and is able to buffering; Reduced the flow velocity of air-flow; Bottom-up the flowing from the bottom after the air cleaner on the short tube 3 of giving vent to anger 4 filters, drains into tail gas and is detained outside the bottle 1 again.And the foam of working as nutrient solution or generation gets into the tail gas hold up vessel with tail gas along air inlet pipe, and the foam of nutrient solution or generation is stayed tail gas and is detained bottle 1, and tail gas then flows from bottom to top, reaches the separation of gas-liquid.
The utility model can summarizing without prejudice to the spirit of the utility model or the concrete form of principal character with other.The above-mentioned embodiment of the utility model all can only be thought explanation rather than the restriction to the utility model; Therefore every essence technology according to the utility model all belongs in the scope of the utility model technical scheme any trickle modification, equivalent variations and modification that above embodiment did.