CN201321462Y - Novel ultrafilter membrane cell culture apparatus - Google Patents
Novel ultrafilter membrane cell culture apparatus Download PDFInfo
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- CN201321462Y CN201321462Y CNU2008200480827U CN200820048082U CN201321462Y CN 201321462 Y CN201321462 Y CN 201321462Y CN U2008200480827 U CNU2008200480827 U CN U2008200480827U CN 200820048082 U CN200820048082 U CN 200820048082U CN 201321462 Y CN201321462 Y CN 201321462Y
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Abstract
The utility model relates to novel ultrafilter membrane cell culture apparatus which comprises a bottle body. Ultrafilter membrane for blocking molecules is arranged inside the bottle body to divide the inner part of the bottle body into a cell growth domain and a cell culture solution domain, a plurality of cell anchorage growth veneers for the anchorage growth of the cells are arranged in the cell growth domain, and culture solution inlet and outlet, cell and target culture product inlets and outlets and a culture product channel which are mutually independent are arranged on the bottle body. The cell culture apparatus has the advantages that the purposes of the rapid increase of the concentration and the amount of the target substance generated by the cells and the highly effective purification are realized, the time for subsequently concentrating and purifying the target culture substance is largely shortened, the dosage of the required reagent is largely reduced, and the cost for scientific research or production can be obviously reduced.
Description
Technical field
The utility model relates to cell culture apparatus field in biomedicine experiment and the bio-pharmaceuticals, is specially a kind of novel ultrafiltration membrane cell incubator.
Background technology
Through vitro culture or be inoculated in macromole biologically active substances such as cultivating the antibody that obtains emiocytosis or generation and polypeptide in the animal body, is an important process of biomedicine experiment and field of biological pharmacy with active somatic cell.And explore new cell culture processes, research and develop new cell culture apparatus is the important subject of biology and pharmaceutical industry with concentration and purity, minimizing required time and the cost that improves target cultivation material always.
Existing cell culture processes is divided into two classes, i.e. culturing in vivo and vitro culture.Culturing in vivo be with cell inoculation in positions such as animal abdominal cavities, utilize the humoral system of animal itself to provide nutrition to target cell.Its advantage is that cell cultivation process is simple to operate, and cost is lower, macromole biologically active substance very high concentrations such as the antibody of emiocytosis or generation and polypeptide, but its significant deficiency is difficult to purifying by obtaining antibody materials, so the now less application of this method.
The cells in vitro cultural method is cultivated cell inoculation for utilizing Tissue Culture Dish, plate and the bottle made with glass or plastics macromolecular material as container in the said vesse that is marked with nutrient solution.Target substances such as antibody and polypeptide can secreted and produce to cell constantly in the fissiparity process; Process a couple of days cultivation is after necrocytosis, obtain containing the supernatant liquor of target substances such as antibody and polypeptide in the centrifugal mode, obtain the purification of target thing of high density by salt precipitation → dialysis → centrifugal → chromatography purification → concentrate once more → complex steps such as dialysis again.Though cells in vitro is cultivated relatively, the antibody materials concentration that obtains extremely low (being generally 0.1～10 microgram/ml level), need through the purification of target thing that extremely complicated concentrates, steps such as dialysis, centrifugal, chromatography just can obtain high density, whole cultivation, results, purifying and concentration process are time-consuming and with high costs, but its cell cultivation process is simple to operate, macromole biologically active substances such as the antibody of emiocytosis or generation and polypeptide are easy to purifying, so widespread use now.
Hollow fiber culture system is the cell culture apparatus of the mid-90 development.This system principle is divided into cell growth/high density target culture and two interfaces of nutrient solution for utilizing tubular fibre with cell culture environment.Cell is target substance such as fissiparity justacrine antibody and polypeptide in the cell growth interface, the nutritive substance in the inner cell nutrient solution of nutrient solution interface can diffuse to the cell growth interface by the micropore on tubular fibre surface and cell in the cell growth interface and target substances such as antibody and polypeptide because molecular weight can't enter the cell growth interface more greatly.Its result contains target substances such as the antibody of high density and polypeptide for nutrient solution in the cell growth interface.Because two all Packed outlets in interface can and be changed fresh medium for harvested cell and target substance, this system can use continuously.But, in operating process, very easily cause pollution, so can't generally apply for over ten years because this device design is complicated.
As the most cells of vitro culture only under " adherent " condition, promptly cell need be attached to vessel surface can the proper splitting growth and secretion produce biologically active substances such as antibody and polypeptide.Therefore, require to be used to produce the plastics polymer surface possess hydrophilic property of Tissue Culture Dish, plate and bottle.And the conventional plastics polymer surface of producing is a hydrophobic property, can't be directly used in the making cell culture container.In China, because the backwardness of macromolecule material surface modification technical study, treatment facility exploitation, the exploitation of cell culture container and device product and production are in space state substantially, the complete dependence on import of product, or adopt the non-once Glass Containers that falls behind.Though the non-once Glass Containers is cheap, because the defective of its cell attachment poor performance, washing difficulty and easy crossed contamination is eliminated abroad already.
Because be subjected to the restriction of gordian technique bottleneck, in decades, cell culture apparatus is the single conventional plastic container of functions of use always.Cultivate finishing the back target, to cultivate material (as monoclonal antibody and polypeptide) concentration extremely low, and follow-uply concentrate, purification step is very complicated, time-consuming and with high costs.Therefore, explore new cell culture processes, research and develop new cell culture apparatus and cultivate concentration, output and the purity of material to improve target, reducing results, the purification of cell cultures and succeeding target thereof cultivation material and concentrating required time and cost is the important subject of biology and pharmaceutical industry always.
The utility model content
The purpose of this utility model is the deficiency that exists at the above cell culture apparatus, provide a kind of simple to operate, be difficult for contaminated, the cell attachment characteristic is good, the optimum simulated conditions of cell growth and secretion biologically active substance and environment can be provided, novel ultrafiltration membrane cell incubator that antibody harvest yield is big.
The utility model is achieved in that novel ultrafiltration membrane cell incubator, comprises bottle, and described bottle inside is provided with the ultra-filtration membrane with molecular retention effect, and ultra-filtration membrane is divided into cell growth functional zone and cell culture fluid functional zone with bottle inside.
Can be provided with the cell attachment growth laminate that several layers is used for supplying the cell attachment growth in the described cell growth functional zone, to increase the surface-area and the adherent rate of cell attachment.
Described bottle is provided with separately independently nutrient solution import and export and cell and import and export of target cultured products and cultured products passage, and cell and target cultured products are imported and exported the biologically active substance that produces for part harvested cell in cell cultivation process; The nutrient solution import and export are for changing cell culture fluid, constantly to replenish the required nutritive substance of cell fission growth; This unique design makes this device become continuous culture system, has improved cell survival and growth cycle.
Described nutrient solution import and export are furnished with the bottle cap of identical size with cell and the import and export of target cultured products, and the bottle cap that nutrient solution is imported and exported is the ventilating cover that is provided with poly-four Buddhist ethene air-permeable envelopes, in order to the convection of air under pollution-free state.
Described ultra-filtration membrane is by ultra-filtration membrane welding or silica gel pad sealing back and firm the merging of bottle inwall, and bottle inside is divided into cell culture fluid functional zone and cell growth functional zone two Room up and down; Described ultra-filtration membrane welding can be that soft ultra-filtration membrane is arranged between two transparent, as to have sieve aperture backing plates; The silica gel pad sealing can be that ultra-filtration membrane is bonded in one with two silica gel pad sealings.
Can be provided with transparent visible area on the described bottle.
The surface applications surface modification of low temperature plasma technology of the cell attachment growth laminate in the internal surface of described bottle and the cell growth functional zone is handled, and makes it change into the tool water-wet behavior by hydrophobic state, thereby strengthens the adherent rate of cell.
The pore size of described ultra-filtration membrane can be 0.05 μ m～1nm, and separable relative molecular mass scope is 500～1,000, the macromole of 000D and colloidal particle, and working pressure can be 0.2～0.4MPa, transmission rates can be 0.5～5m
3/ (m
2D).
Described nutrient solution import is provided with the bottle side wall middle part, and mouthful wall tilts to stretch out sidewall, directly communicates with the cultivation liquid zone; Cell and target cultured products are imported and exported the offside that is arranged on the nutrient solution import, in sink into the edge of bottle, pipeline passes to be cultivated the liquid zone and enters cell growth functional zone.
After described cell attachment growth laminate and ultra-filtration membrane process ultra-filtration membrane welding or the silica gel pad sealing is by ultrasonic seamless welding technique, is embedded in securely in the plastic material of Tissue Culture Flask.
This reality should novelly possess following characteristics: (1) is answered the ultra-filtration membrane of apparatus molecular retention effect that Tissue Culture Flask is divided into two and is cultivated the interface; (2) macromolecular material of constructing cell growth functional zone carries out surface modification treatment with the surface modification of low temperature plasma technology, increases the adherent rate of cell; (3) construct the multilayered structure that cell growth functional zone are provided with cell attachment growth laminate, greatly increase the surface-area of the macromolecular material of constructing cell growth functional zone; (4) design independently imported and exported of Du Te functional zone can be cultivated material for regular harvested cell and target, changes and replenishes cell culture fluid, makes this device become a continuous culture system like this.The utility model has been inherited the advantage that macromole target substance (as monoclonal antibody) that traditional extracorporeal culturing method gathers in the crops is easy to purifying, can obtain the characteristic of the high density target substance that similar live body cultivates again, also keep the feature that traditional product is convenient to the observation of cell growth conditions simultaneously.The utility model has realized that cell produces the quick increase of target substance concentration and total amount and the purpose of efficiently purifying, and shorten succeeding target greatly and cultivate the time that material concentrates and purifying is required and reduce required reagent dosage, obviously reduce scientific research or production cost.
Description of drawings
Fig. 1 is a three-dimensional structure diagram of the present utility model;
Fig. 2 is an A-A cross-sectional view of the present utility model;
Table 3 is the relative merits contrast table of the utility model and existing cell cultures instrument;
Table 4 is the technical indicator comparison sheet of the utility model and conventional Tissue Culture Flask;
Table 5 is the economic target comparison sheet that the utility model and conventional Tissue Culture Flask are produced monoclonal antibody.
Wherein, 1: cell and target cultured products are imported and exported; 2: bottle; 3: nutrient solution is imported and exported; 4: ultra-filtration membrane; 5: the cultured products passage; 6: cell attachment growth laminate; 7: cell growth functional zone; 8: the cell culture fluid functional zone; 9: visible area.
Embodiment
Come the utility model ultrafiltration membrane cell incubator is described in detail below in conjunction with accompanying drawing and specific embodiment.
Novel ultrafiltration membrane cell incubator as depicted in figs. 1 and 2, comprises bottle 2, and described bottle 2 inside are provided with the ultra-filtration membrane 4 with molecular retention effect, and ultra-filtration membrane 4 is divided into cell growth functional zone 7 and cell culture fluid functional zone 8 with bottle inside; Cell growth functional zone 7 are positioned at the lower floor of bottle 2, and cell culture fluid functional zone 8 are positioned at the upper strata of bottle 2 inner cells growth functional zone 8.In cell growth functional zone 7, be provided with several layers and use and the laminate 6 of growing for the cell attachment of cell attachment growth, to increase the surface-area and the adherent rate of cell attachment.Bottle 2 is provided with specially independently nutrient solution import and export 1, cell and import and export 3 of target cultured products and cultured products passage 5, and cultured products passage 5 is the passage of forming between the cell attachment growth laminate 6.Cell and target cultured products are imported and exported 3 biologically active substances for the generation of part harvested cell in cell cultivation process; Nutrient solution import and export 3 are for changing cell culture fluid, constantly to replenish the required nutritive substance of cell fission growth; This unique design makes this device become continuous culture system, has improved cell survival and growth cycle.Nutrient solution import and export 3 and cell and target cultured products are imported and exported 1 and all are furnished with bottle cap, and the bottle cap that nutrient solution is imported and exported is the ventilating cover that is provided with polyfluortetraethyleventilated ventilated membrane, in order to the convection of air under pollution-free state.Nutrient solution import 3 is provided with the bottle side wall middle part, and mouthful wall tilts to stretch out sidewall, directly communicates with the cultivation liquid zone; Cell and target cultured products are imported and exported 1 offside that is arranged on the nutrient solution import, in sink into the edge of bottle, pipeline passes to be cultivated the liquid zone and enters cell growth functional zone.Nutrient solution import and export 3 are identical with the bottle cap size with the bottleneck diameter of cell and target cultured products import and export 1.Bottle 2 is provided with transparent visible area 10.The internal surface of bottle 2 and cell growth functional zone inner cell adherent growth laminate 6 application of cold temperature plasma surface modification technologies were handled, and made it change into the tool water-wet behavior by hydrophobic state, thereby strengthened the adherent rate of cell.
Ultra-filtration membrane 4 is by ultra-filtration membrane welding or silica gel pad sealing back and firm the merging of bottle inwall, and bottle inside is divided into cell culture fluid functional zone 8 and cell growth functional zone, and about in the of 8 two Room; The ultra-filtration membrane welding is that soft ultra-filtration membrane is arranged between two transparent, as to have sieve aperture backing plates; Silica gel pad sealing is that ultra-filtration membrane is bonded in the one silica gel pad with two silica gel pads sealings and is provided with sieve aperture.Cell attachment growth laminate 6 and ultra-filtration membrane 4 can be by ultrasonic seamless welding technique after through ultra-filtration membrane weldings or silica gel pad sealing, admittedly be embedded in the plastic material of Tissue Culture Flask.Ultrasonic seamless welding internals with ultrasonic bonding machine do not add under any welding compound condition with bottle on firm the merging of lower body; Its processing condition are: frequency 20k, weld interval: 0.1 ", the dwell time: 0.6 ", air pressure: 30psi, highly: 30cm.
The pore size of ultra-filtration membrane 4 can be from 0.05 μ m～1nm, and separable relative molecular mass scope is 500～1,000, the macromole of 000D and colloidal particle, and working pressure is generally 0.2～0.4MPa, and transmission rates is 0.5～5m
3/ (m
2D).Select for use ultra-filtration membrane, the IgM in 20kd molecular retention aperture to select the ultra-filtration membrane in 90kd molecular retention aperture etc. for use as antagonist IgG.
The low-temperature plasma surface treatment is introduced oxygen, nitrogen or other plasma gass for dried bottle is put into the plasma treatment device under vacuum or negative pressure state, the plasma generator discharge forms plasmasphere at the cell culture container internal surface; In treating processes, its processing condition are: operating pressure 50～900 milli tons, 100～500 watts of powers, 100～500 seconds treatment times.Preferred processing condition is: with the power connection of plasma processor, set processing parameter: operating pressure is 50 milli tons, treatment time is 100 seconds, power is 500 watts, connect oxygen and pressurized air, then pretreated cell culture container is put into plasma processor, handle on the surface of pair cell culture vessel.By the Cement Composite Treated by Plasma macromolecular material, introduce new group selectively on the surface, change surface moist, surface potential, surface energy polar component and dispersive component and surface micro-structure, reach the purpose of improving the macromolecular material biocompatibility, can obtain the surface of a series of DIFFERENT WET lubricant natures thus, be applicable to bio-medical material as the specific occasion.Plasma gas is meant and utilizes non-polymerization gas (as Ar, N
2, CO, NH
4, O
2, H
2Deng) plasma body.Plasma body and polymer surface interact, make and form new functional group from the teeth outwards and change polymer chain structure, improving parent's (dredging) water-based, cementability, surperficial electric property, optical property and biocompatibility etc., thereby reach the purpose of surface modification.In plasma treatment procedure, the discharging condition with different often act as the master with certain, and several effects are also deposited.The advantage of Low Temperature Plasma Treating is that effect is remarkable, and technology is simple, and is pollution-free, can obtain different surface propertys, applied range by changing different treatment condition.What is more important, treatment effect is confined to the surface and does not influence the material body performance.Low-temperature plasma has on the one hand that enough high-octane active specy makes that reactant molecule excites, ionization or scission of link, can not make treated material pyrolysis or ablation on the other hand, has unique using value aspect the modified polymer material surface.
Add their confirmation by following inspection procedure through the growth of the cell attachment after low-temperature plasma surface treatment laminate treatment effect:
1, reference to standard and principle:
Reference to standard: ASTM Standard D2578;
Principle of work: the mensuration of frosting energy (surface tension) is according to DIN ISO8296 by test ink, be to intend brushing the long ink-stick of about 100mm on the film of surveying with the China ink of known different surfaces energy, and observe its ink-stick limit more than 90% and in 2 seconds, whether shrink and form ink droplet, if any, then change the China ink of low one-level surface energy and brush ink-stick again, carry out same observation, until not shrinking and ink droplet occurring, the surface energy of this test ink promptly should be the surface energy of this product relatively, and this method can accurately be measured the surface tension of base material.
2, test method:
Product takes out at once according to the AQL sampling value and takes out the product that needs test after handling through surface treating machine, with dyne examination pen (60 dynes per centimeter) test cell adherent growth surface.When test pen marks a line at the frosting of handling, if become line continuously and in one minute no any shrinkage phenomenon then for qualified.(illustrate: this material surface energy is not less than dynes per centimeter, as not being linked to be line intermittently, this material surface energy is described less than 60mN/m, undertreatment or even be untreated, do not meet adherent processing requirements.)
3, instrument and apparatus
Dyne pen, vernier scale, tool microscope, projector.
The utility model is used the ultra-filtration membrane with molecular retention effect cell culture container is divided into two functional zone, forms cell growth/target and cultivates material interface and nutrient solution interface.In cell growth/target was cultivated material interface, cell and target were cultivated material (as monoclonal antibody and polypeptide) and are accumulated in this district by obstruct owing to molecular weight is big; Products of cellular metabolism in nutritive substance in the cell culture fluid interface and the cell growth functional zone can be because of osmotic pressure and concentration difference unrestricted flow because molecular weight is little.Use plasma surface modification technology in addition and handle, make it change into the tool water-wet behavior, thereby strengthen the adherent rate of cell by hydrophobic state to constructing the adherent plastics polymer surface of cell attachment.Because above-mentioned two kinds of The Application of Technology, compare with the present conventional Tissue Culture Flask that uses, following variation has taken place product structure: this project product application ultra-filtration membrane is divided into two physical function districts with cell culture container, these two functional zone are not only separate but also interconnect, cell and target cultivate material (as monoclonal antibody and polypeptide) because molecular weight is big is accumulated in this district by obstruct, makes target cultivation material concentration in this district be higher than tens of times of ordinary cells culturing bottle persons to hundreds of times; Products of cellular metabolism in nutritive substance in the cell culture fluid interface and the cell growth functional zone is because molecular weight is little, can be because of osmotic pressure and concentration difference unrestricted flow, thus realize that cell growth/target cultivates the constantly additional serial dilution with products of cellular metabolism of nutritive substance in the material universe functional zone.In cell growth functional zone, increased the multilayer veneer structure of growing for cell attachment, all handle with plasma surface modification technology on the laminate surface, has increased the surface-area and the adherent rate of cell attachment back up pad.Cell growth/the target of cell culture container cultivation material and two functional zone of nutrient solution are all designed and independently passed in and out draining hole separately in addition: cell growth/target is cultivated the biologically active substance that material functional zone draining hole produces for part harvested cell in cell cultivation process; Nutrient solution functional zone draining hole is for changing cell culture fluid, constantly to replenish the required nutritive substance of cell fission growth.This unique design makes this device become continuous culture system, has improved cell survival and growth cycle.
Compare with the device of existing cell cultures, 26S Proteasome Structure and Function of the present utility model is more perfect, break through traditional Tissue Culture Flask fully only as the container that the cell growing environment is provided, cultured continuously and the function that obtains high density, highly purified target cultivation material have been increased, realized that cell produces the quick increase of target substance concentration and total amount and the purpose of efficiently purifying, reduced cell cultures and succeeding target thereof significantly and cultivate the results of material, purification and concentrate required time and cost.Shown in the table specific as follows:
(1) be the relative merits contrast table of the utility model and existing cell cultures instrument:
(2) be the technical indicator comparison sheet of the utility model and conventional Tissue Culture Flask:
| Index | This project product | Conventional Tissue Culture Flask |
| Functional zone (individual) | 2 | 1 |
| Liquid entrance (individual) | 2 | 1 |
| Ultra-filtration membrane (molecular retention effect) | Have | Do not have |
| Cell attachment aspect (layer) | 9 | 1 |
| The cell attachment aspect is amassed (Cm 2) | 1770 | 234 |
| The nutrient solution body bulk (integral body, ml) | 576 | 288 |
| Cell cultures and target culture functional zone volumes (ml) | 230 | -- |
| Cell density (cell/ml) | 5×10^7 | 1×10^6 |
| Antibody concentration (mg/ml) | 1.50 | 0.02 |
| Antibody harvest yield (mg) | 345 | 5.76 |
(3) be the economic target comparison sheet that the utility model and conventional Tissue Culture Flask are produced monoclonal antibody:
Claims (10)
1, novel ultrafiltration membrane cell incubator comprises bottle, it is characterized in that: described bottle inside is provided with the ultra-filtration membrane with molecular retention effect, and ultra-filtration membrane is divided into cell growth functional zone and cell culture fluid functional zone with bottle inside.
2, novel ultrafiltration membrane cell incubator as claimed in claim 1 is characterized in that: be provided with the cell attachment growth laminate that several layers is used for supplying the cell attachment growth in the described cell growth functional zone.
3, novel ultrafiltration membrane cell incubator as claimed in claim 2 is characterized in that: described bottle is provided with special nutrient solution import and export and cell and target cultured products and imports and exports and the cultured products passage.
4, novel ultrafiltration membrane cell incubator as claimed in claim 3, it is characterized in that: described nutrient solution import and export are furnished with the bottle cap of identical size with cell and the import and export of target cultured products, and the bottle cap that nutrient solution is imported and exported is the ventilating cover that is provided with poly-four Buddhist ethene air-permeable envelopes.
5, novel ultrafiltration membrane cell incubator as claimed in claim 4, it is characterized in that: described ultra-filtration membrane is by ultra-filtration membrane welding or silica gel pad sealing back and firm the merging of bottle inwall, and bottle inside is divided into cell culture fluid functional zone and cell growth functional zone two Room up and down; Described ultra-filtration membrane welding can be that soft ultra-filtration membrane is arranged between two transparent, as to have sieve aperture backing plates; The silica gel pad sealing can be that ultra-filtration membrane is bonded in one with two silica gel pad sealings.
6, novel ultrafiltration membrane cell incubator as claimed in claim 4 is characterized in that: described bottle is provided with transparent visible area.
7, novel ultrafiltration membrane cell incubator as claimed in claim 1 is characterized in that: the surface applications surface modification of low temperature plasma technology of the cell attachment growth laminate in the internal surface of described bottle and the cell growth functional zone was handled.
8, novel ultrafiltration membrane cell incubator as claimed in claim 1, it is characterized in that: the pore size of described ultra-filtration membrane is 0.05 μ m～1nm, separable relative molecular mass scope is 500～1,000, the macromole of 000D and colloidal particle, working pressure is 0.2～0.4MPa, and transmission rates is 0.5～5.m
3/ (m
2D).
9, novel ultrafiltration membrane cell incubator as claimed in claim 4 is characterized in that: described nutrient solution import is provided with the bottle side wall middle part, and mouthful wall tilts to stretch out sidewall, directly communicates with the cultivation liquid zone; Cell and target cultured products are imported and exported the offside that is arranged on the nutrient solution import, in sink into the edge of bottle, pipeline passes to be cultivated the liquid zone and enters cell growth functional zone.
10, as claim 2 or 5 described novel ultrafiltration membrane cell incubators, it is characterized in that: after described cell attachment growth laminate and ultra-filtration membrane process ultra-filtration membrane welding or the silica gel pad sealing is by ultrasonic seamless welding technique, is embedded in securely in the plastic material of Tissue Culture Flask.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008200480827U CN201321462Y (en) | 2008-05-21 | 2008-05-21 | Novel ultrafilter membrane cell culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008200480827U CN201321462Y (en) | 2008-05-21 | 2008-05-21 | Novel ultrafilter membrane cell culture apparatus |
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| CN201321462Y true CN201321462Y (en) | 2009-10-07 |
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| CNU2008200480827U Expired - Lifetime CN201321462Y (en) | 2008-05-21 | 2008-05-21 | Novel ultrafilter membrane cell culture apparatus |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108410821A (en) * | 2018-03-14 | 2018-08-17 | 苏杰 | A kind of medical human erythrocyte cultural method |
| CN109266548A (en) * | 2018-10-11 | 2019-01-25 | 广州洁特生物过滤股份有限公司 | A kind of dynamic cell culture device and cell culture system |
| CN110669672A (en) * | 2019-10-14 | 2020-01-10 | 东莞市东阳光诊断产品有限公司 | Microfluidic chip for producing antibody |
| CN112063502A (en) * | 2020-09-14 | 2020-12-11 | 于爱玲 | Biological culture device based on hemodialyzer |
-
2008
- 2008-05-21 CN CNU2008200480827U patent/CN201321462Y/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108410821A (en) * | 2018-03-14 | 2018-08-17 | 苏杰 | A kind of medical human erythrocyte cultural method |
| CN109266548A (en) * | 2018-10-11 | 2019-01-25 | 广州洁特生物过滤股份有限公司 | A kind of dynamic cell culture device and cell culture system |
| CN109266548B (en) * | 2018-10-11 | 2022-04-05 | 广州洁特生物过滤股份有限公司 | Dynamic cell culture device and cell culture system |
| CN110669672A (en) * | 2019-10-14 | 2020-01-10 | 东莞市东阳光诊断产品有限公司 | Microfluidic chip for producing antibody |
| CN112063502A (en) * | 2020-09-14 | 2020-12-11 | 于爱玲 | Biological culture device based on hemodialyzer |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Guangzhou Jet Bio-Filtration Products Co., Ltd. Assignor: Yuan Jianhua|Yuan Ye Contract record no.: 2010440001241 Denomination of utility model: Novel ultrafiltration membrane cell incubator and manufacture method thereof Granted publication date: 20091007 License type: Exclusive License Record date: 20100819 |
|
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Guangzhou Jet Bio-Filtration Products Co., Ltd. Assignor: Yuan Jianhua|Yuan Ye Contract record no.: 2010440001241 Denomination of utility model: Novel ultrafiltration membrane cell incubator and manufacture method thereof Granted publication date: 20091007 License type: Exclusive License Record date: 20100819 |
|
| LICC | Enforcement, change and cancellation of record of contracts on the licence for exploitation of a patent or utility model | ||
| ASS | Succession or assignment of patent right |
Owner name: GUANGZHOU JET BIOLOGICAL FILTRATION CO., LTD. Free format text: FORMER OWNER: YUAN JIANHUA Effective date: 20150728 Free format text: FORMER OWNER: YUAN YE Effective date: 20150728 |
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| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20150728 Address after: 511356, No. 1, beat Tong Road, Yonghe Economic Zone, Guangzhou economic and Technological Development Zone, Guangdong, Guangzhou Patentee after: Guangzhou Jie Te biofiltration limited-liability company Address before: 510000 Friendship Road 173, Guangzhou economic and Technological Development Zone, Guangdong, Guangzhou Patentee before: Yuan Jianhua Patentee before: Yuan Ye |
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| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20091007 |