CN102899242A - Multifunctional test tube for cultivating bacteria and using method of multifunctional test tube - Google Patents
Multifunctional test tube for cultivating bacteria and using method of multifunctional test tube Download PDFInfo
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- CN102899242A CN102899242A CN2012104344803A CN201210434480A CN102899242A CN 102899242 A CN102899242 A CN 102899242A CN 2012104344803 A CN2012104344803 A CN 2012104344803A CN 201210434480 A CN201210434480 A CN 201210434480A CN 102899242 A CN102899242 A CN 102899242A
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
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Abstract
The invention discloses a multifunctional test tube for cultivating bacteria. The multifunctional test tube comprises a test tube main body and a test tube cover, wherein the test tube main body comprises an upper part of the test tube main body, a lower part of the test tube main body and a connection part of the upper part and the lower part of the test tube main body; the lower part of the test tube main body is a circular cylinder; the upper part of the test tube main body is a square cylinder; and the bottom part of the test tube main body is U-shaped or flat. Besides, the invention also discloses a method for using the multifunctional test tube. By the multifunctional test tube, the bacteria can be taken out without opening the cover in the process of bacteria cultivation, and morphologies of bacteria in the test tube can be observed under a microscope directly; biohazards of infectious pathogenic microorganisms, culture pollution and volatilization of culture mediums are avoided; and bacteria growth indicator can be added into the multifunctional test tube and then the growth of the bacteria can be automatically monitored by using an instrument, particularly the growth of the bacteria in large-scale samples can be monitored.
Description
Technical field
The present invention relates to a kind of test tube, particularly relate to a kind of Multifunctional test tube for culturing bacterium, especially the applicable test tube that needs to observe the bacterial growth form, need in the automatic bacterial detection growth instrument to be specially adapted to the test tube of ne ar observation, also to be applicable to need to calculate the test tube of bacterial clone concentration in the liquid culture environment.In addition, the invention still further relates to the using method of this Multifunctional test tube.
Background technology
Existing test tube for the use of liquid nutrient medium microbial culture, normally right cylinder or square body.Because refractive power is too strong, existing tubular test tube can't be used for the observation of microscopically liquid nutrient medium ne ar; Because the test tube of the existing square column bodily form has corner angle, the instrument that is not easy to the bacterial growth indicator detects, and especially is not easy to instrument and automatically detects large-scale sample.Therefore, we need to a kind ofly can satisfy the Multifunctional test tube that requires below simultaneously: 1. can be used at any time the observation of microscopically test tube ne ar; Need not to uncap and get bacterium, solved simultaneously the problem of pollution and Biosafety; 2. can be used for full-automatic instrument on a large scale according to the variation monitoring bacterial growth situation of bacterial growth indicator; 3. can calculate bacterial colony quantity.
Summary of the invention
Technical problem to be solved by this invention provides a kind of Multifunctional test tube for culturing bacterium, this test tube can be used for the cultivation of bacterium, and need not in culturing process uncaps gets bacterium, directly examine under a microscope the form of bacterium in the pipe, also can add the bacterial growth indicator and automatically monitor the growing state of bacterium with instrument, especially the monitoring of bacterial growth situation in the extensive sample can be carried out, the quantity of the inner bacterial clone of test tube can be calculated according to the scale on the Multifunctional test tube tube wall or external accessory scale.For this reason, the present invention also provides the using method of this Multifunctional test tube.
For solving the problems of the technologies described above, the invention provides a kind of Multifunctional test tube for culturing bacterium, this Multifunctional test tube comprises test tube main body and test tube cap; Described test tube main body comprises the connection portion of test tube main body top, test tube lower body part and test tube main body upper and lower; Described test tube lower body part is right cylinder, and described test tube main body top is square body; Described test tube bottom part body is " U " shape or flat.
The cross section of described test tube main body top square body can be square or rectangular; Can be carved with on the square body tube wall of described test tube main body top for the scale that calculates bacterial clone quantity, perhaps can use and be with graduated external accessory (for example graduated scale) to calculate bacterial clone quantity.
Preferably, the cross section of described test tube main body top square body to angular diameter less than or equal to the cylindrical diameter of test tube lower body part.
Preferably, the diameter of the connection portion of described test tube main body top and bottom is less than or equal to the maximum diameter of test tube main body top and test tube lower body part.
Arbitrary shape can be adopted in the connection portion of described test tube main body top and bottom.Preferably, the connection portion of described test tube main body top and bottom can be right cylinder, this cylindrical diameter less than or equal to the cross section on test tube main body top to angular diameter.Preferably, the connection portion of described test tube main body top and bottom can be irregular body, the connection test tube main body top of this connection portion and the diameter at two ends, bottom are larger, and two ends diminish gradually to the diameter of middle portion, and the cross section of this connection portion is the arc of depression.
Preferably, described test tube cap is a kind of airtight, ventilative test tube cap, can prevent that liquid and bacterium from spilling, but air can asepticly be come in and gone out, the micron-sized hydrophobic filter membrane of pad in this test tube cap, preferred 0.2 micron hydrophobic filter membrane (0.2 micron hydrophobic filter membrane can isolate bacteria).
In addition, the present invention also provides a kind of using method that should be used for the Multifunctional test tube of culturing bacterium, comprises the steps:
The first step, test tube guarantee that through sterilization process the test tube body interior of sealing is sterile state;
Second step is opened test tube cap, adds nutrient solution, and adding needs the sputum sample that detects, the sample of other type again, covers tightly test tube cap, puts into suitable culture environment and carries out microbial culture;
The 3rd step, according to the characteristics of bacterial growth, take out test tube in the specific time, the direct viewing specific form of bacterium in the liquid nutrient medium in vitro under inverted microscope, determining whether the growth of being examined bacterium, with and growing state.
Preferably, before the first step, can add following steps: open test tube cap, add the bacterial growth indicator in the test tube bottom.After the 3rd step, increase following steps: according to the scale on the test tube square body part tube wall, can count out the bacterial colony quantity in the scale card; Then calculate bacterial colony concentration according to the bacterial colony quantity in the respective volume.
In second step, described suitable culture environment comprises that constant incubator, automatic bacterial are cultivated detector, manually microbial culture detector, full-automatic mycobacterium are cultivated detector, manually mycobacterium is cultivated detector.
Be compared with existing technology, beneficial effect of the present invention is: the present invention can be convenient to the variation of instrument bacterial detection growth indicator by " U " shape or flat test tube bottom part body.The present invention can make microscopically can observe ne ar (having overcome existing right cylinder test tube because refractive power can't be used for too by force the defective of the observation of microscopically liquid nutrient medium ne ar) by the square body part of test tube.The present invention can prevent that by test tube cap liquid and bacterium from spilling, but gas can need for bacterial growth in aseptic discrepancy.Multifunctional test tube of the present invention need not to uncap in the microbial culture process and gets bacterium, and directly the form of bacterium in microscopically just can observation tube has been avoided the biological hazard of infectious pathogenic microorganisms, also prevents the pollution of culture, has also avoided the substratum volatilization.Also the bacterial growth indicator is monitored bacterium automatically with instrument growing state can be in Multifunctional test tube, added, especially the monitoring of bacterial growth situation in the extensive sample can be carried out.Can calculate the in vitro concentration of bacterial clone by the scale of square body part or with graduated external accessory (such as graduated scale).The Multifunctional test tube that the present invention is used for culturing bacterium is particularly suitable for the cultivation, evaluation of the pathogenic micro-organisms such as infective bacterial, virus and drug susceptibility is detected using, for example cultivation of mycobacterium tuberculosis complex, evaluation and drug susceptibility detected use.The present invention also is suitable for sterile culture and the culture morphologic observation is used, and for example the cultivation of suspension cell and morphologic observation are used.
Description of drawings
Fig. 1 a and Fig. 1 b be bottom of the present invention for " U " structural representation of Multifunctional test tube of shape; Wherein, Fig. 1 a is that the connection portion on test tube lower body part and top is column; Fig. 1 b is that the connection portion on test tube lower body part and top is irregular body.
Fig. 2 a and Fig. 2 b are that bottom of the present invention is the structural representation of flat Multifunctional test tube; Wherein, Fig. 2 a is that the connection portion on test tube lower body part and top is column; Fig. 2 b is that the connection portion on test tube lower body part and top is irregular body.
Fig. 3 is with the structural representation of the Multifunctional test tube of scale on the column tube wall of the present invention.
Fig. 4 a, Fig. 4 b, Fig. 4 c and Fig. 4 d are the specificity strand form schematic diagram that divides the mycobacterium tuberculosis complex of observing among the embodiment 1,2,3 under the inverted microscope in Multifunctional test tube square body shaped part of the present invention; Wherein, Fig. 4 a represents the schematic diagram of 40 times of magnifications; Fig. 4 b represents the schematic diagram of 100 times of magnifications; Fig. 4 c represents the schematic diagram of 200 times of magnifications; Fig. 4 d represents the schematic diagram of 400 times of magnifications.
Description of reference numerals among the figure:
1 is test tube cap; 2 is test tube main body top; The connection portion of 3 test tube main body tops and bottom; 4 is the test tube lower body part; 5 is the test tube bottom part body; 6,7 for being used for calculating the scale of bacterial clone quantity.
Embodiment
See also Fig. 1 a, Fig. 1 b, Fig. 2 a, Fig. 2 b, test tube of the present invention comprises: test tube main body and test tube cap.
The test tube main body, usually made by plastics or glass or other optically transparent material, the test tube main body comprises the connection portion 3 of test tube main body top 2, test tube lower body part 4 and test tube main body top and bottom, test tube main body top 2 is that square body, test tube lower body part 4 are right cylinder, and the cross section on test tube main body top 2 is square or rectangulars.The cross section (square or rectangular) on test tube main body top 2 to the cylinder diameter of angular diameter less than or equal to test tube lower body part 4.Test tube bottom part body 5 can for " U " shape (sees Fig. 1 a, Fig. 2 a) or for flat (Fig. 1 b, Fig. 2 b).The connection portion 3 of test tube main body top and bottom can take different shapes structure, for example, cylindrical shapes are adopted in connection portion 3, this cylindrical diameter less than or equal to the cross section (square or rectangular) on test tube main body top 2 to angular diameter (seeing Fig. 1 a, Fig. 1 b).And for example, connection portion 3 is irregular body, and the diameter at the connection test tube main body top of connection portion 3 and the two ends of bottom is larger, and two ends diminish gradually to the diameter of middle portion, and the cross section of connection portion 3 is the arc of depression (seeing Fig. 2 a, Fig. 2 b); The diameter of the connection portion 3 of two kinds of forms of described test tube main body is less than or equal to the maximum diameter of test tube main body top 2 with test tube lower body part 4.
Test tube cap 1 is the sealing cover that airing function is arranged, and can prevent that liquid and bacterium from spilling, but air can asepticly be come in and gone out.The micron-sized hydrophobic filter membrane of test tube cap 1 interior pad, preferred 0.2 micron hydrophobic filter membrane (0.2 micron hydrophobic filter membrane can isolate bacteria) provides the aseptic gas exchange.
Another embodiment of Multifunctional test tube of the present invention as shown in Figure 3, be carved with on the tube wall on test tube main body top 2 for the scale 6,7 that calculates bacterial clone quantity, test tube main body top 2 be square cylinder (for example, the length of side of square cylinder is 1 centimetre), scale 7(is arranged for example on the tube wall of square cylinder, scale 7 is of a size of 10 millimeters * 10 millimeters), scale 6(is arranged for example on the adjacent facets tube wall, scale 6 is of a size of 10 millimeters).
The below enumerates embodiment and specifies the detailed process that Multifunctional test tube of the present invention is used for the microbial culture evaluation:
Embodiment 1 test tube of the present invention is as follows in the process of the culture ﹠ identification of full-automatic mycobacterium cultivation detecting instrument participation mycobacterium tuberculosis complex
The 1st step, open test tube cap, the test tube bottom adds the bacterial growth indicator, covers tightly test tube cap.Through sterilization process, guarantee that the test tube body interior of sealing is sterile state.
The 2nd step, open test tube cap, add nutrient solution, add again sputum sample, other type sample or the mycobacterium tuberculosis complex that needs detection, cover tightly test tube cap, put into suitable culture environment and carry out microbial culture, for example the BACTEC MGIT 960 of U.S. company BD production, the full-automatic mycobacteriums of 320 series are cultivated detecting instruments, and the automatic bacterial that French Mei Liai company produces is cultivated detecting instrument etc.
In the 3rd step, in the training period, the bottom of the automatic test tubes of instrument has determined whether grower according to the variation of bacterial growth indicator.Prompting according to instrument, take out test tube, the test tube square body part of under inverted microscope, observing, detect the wherein specific strand form of mycobacterium tuberculosis complex (seeing Fig. 4 a, 4b, 4c, 4d), whether to judge grower as mycobacterium tuberculosis complex, can observe its growth conditions simultaneously.Need not to uncap and draw bacterium, just can examine under a microscope the growthhabit of bacterium, avoided the biological hazard of infectious pathogenic microorganisms, also prevent the pollution of culture, also avoided the substratum volatilization.
In the 4th step, according to the scale on the test tube square body part tube wall, can count out the bacterial colony quantity in the scale card; Then calculate bacterial colony concentration according to the bacterial colony quantity in the respective volume.
The process that embodiment 2 test tubes of the present invention are cultivated detecting instrument participation mycobacterium tuberculosis complex culture ﹠ identification manual mycobacterium is as follows
The 1st step, open test tube cap, the test tube bottom adds the bacterial growth indicator, covers tightly test tube cap.Through sterilization process, guarantee that the test tube body interior of sealing is sterile state.
The 2nd step, open test tube cap, add nutrient solution, add again sputum sample, other type sample or the mycobacterium tuberculosis complex that needs detection, cover tightly test tube cap, put into suitable culture environment and carry out microbial culture, for example the manual mycobacterium of the BACTEC MGIT of U.S. company BD production is cultivated detecting instrument, the manual microbial culture detecting instrument that French Mei Liai company produces etc.
The 3rd step, according to the instrument operation instruction, take out test tube in the specific time, at first by the variation of manual detecting instrument test tubes bottom bacterial growth indicator, determined whether grower; The test tube square body part of then under inverted microscope, observing, detect the wherein specific strand form of mycobacterium tuberculosis complex (seeing Fig. 4 a, 4b, 4c, 4d), whether to judge grower as mycobacterium tuberculosis complex, can observe its growth conditions simultaneously.Need not to uncap and draw bacterium, just can examine under a microscope the growthhabit of bacterium, avoided the biological hazard of infectious pathogenic microorganisms, also prevent the pollution of culture, also avoided the substratum volatilization.
In the 4th step, according to the scale on the test tube square body part tube wall, can count out the bacterial colony quantity in the scale card; Then calculate bacterial colony concentration according to the bacterial colony quantity in the respective volume.
In the 1st step, test tube guarantees that through sterilization process the test tube body interior of sealing is sterile state.
The 2nd step, open test tube cap, add nutrient solution, adding needs sputum sample, other type sample or mycobacterium tuberculosis complex of detecting again, covers tightly test tube cap, puts into suitable culture environment and carries out microbial culture, such as traditional constant incubator etc.
The 3rd step, characteristics according to bacterial growth, take out test tube in the specific time, the test tube square body part of under inverted microscope, observing, detect the wherein specific strand form of mycobacterium tuberculosis complex (seeing Fig. 4 a, 4b, 4c, 4d), to determine whether the growth of mycobacterium tuberculosis complex, can observe its growth conditions simultaneously.Observing complete test tube can be reentered into and proceed in the testing environment to cultivate.Need not to uncap and draw bacterium, just can examine under a microscope the growthhabit of bacterium, avoided the biological hazard of infectious pathogenic microorganisms, also prevent the pollution of culture, also avoided the substratum volatilization.
In the 4th step, according to the scale on the test tube square body part tube wall, can count out the bacterial colony quantity in the scale card; Then calculate bacterial colony concentration according to the bacterial colony quantity in the respective volume.
The process of the culture ﹠ identification of embodiment 4 test tubes participation mycobacteriums of the present invention is as follows
In the 1st step, test tube guarantees that through sterilization process the test tube body interior of sealing is sterile state.
The 2nd step, open test tube cap, add nutrient solution, add again sputum sample, other type sample or the mycobacterium that needs detection, cover tightly test tube cap, put into suitable culture environment and carry out microbial culture, cultivate detector, manual mycobacterium cultivation detector etc. such as traditional constant incubator, automatic bacterial cultivation detector, manual microbial culture detector, full-automatic mycobacterium.
In the 3rd step, according to the characteristics of bacterial growth, take out test tube in the specific time, the test tube square body part of under inverted microscope, observing, detect the wherein form of mycobacteria specific, to determine whether the growth of being examined mycobacterium, can observe its growth conditions simultaneously.Observing complete test tube can be reentered into and proceed in the testing environment to cultivate.Need not to uncap and draw bacterium, just can examine under a microscope the growthhabit of bacterium, avoided the biological hazard of infectious pathogenic microorganisms, also prevent the pollution of culture, also avoided the substratum volatilization.
In the 4th step, according to the scale on the test tube square body part tube wall, can count out the bacterial colony quantity in the scale card; Then calculate bacterial colony concentration according to the bacterial colony quantity in the respective volume.
The process that embodiment 5 test tubes of the present invention participate in the antibacterials sensitivity Detection is as follows
The 1st step, the square body of test tube partly be square cylinder (for example, the length of side of square cylinder is 1 centimetre), scale 7(is arranged for example on the tube wall of square cylinder, scale 7 is of a size of 10 millimeters * 10 millimeters), scale 6(is arranged for example on the adjacent facets tube wall, scale 6 is of a size of 10 millimeters), see Fig. 3.
In the 2nd step, test tube guarantees that through sterilization process the test tube body interior of sealing is sterile state.
In the 3rd step, set growth control pipe and drug testing pipe; Open test tube cap, add the fixed amount nutrient solution; Add fixed amount sputum sample, other type sample of needs detection or examined bacterium according to antibacterials sensitivity Detection rules; Add antibacterials in the drug testing pipe; Cover tightly test tube cap, put into suitable culture environment and carry out microbial culture, cultivate detector, manual mycobacterium cultivation detector etc. such as traditional constant incubator, automatic bacterial cultivation detector, manual microbial culture detector, full-automatic mycobacterium.
The 4th step, according to the characteristics of bacterial growth, take out test tube in the specific time, the test tube square body part of observing under inverted microscope detects the specific shape of wherein being examined bacterium, to determine whether the growth of being examined bacterium.
In the 5th step, the bacterial clone quantity on the scale card of 3 millimeters * 9 millimeters of microscopically countings is simultaneously measured the height of intraluminal fluid face by the scale on the adjacent facets tube wall, calculate the bacterial clone concentration in the respective volume.Can be examined bacterium to susceptibility and the resistance of this medicine with judgement according to the different of bacterial clone concentration in growth control pipe and the drug testing pipe.Need not to uncap and draw bacterium, just can examine under a microscope the growthhabit of bacterium, avoided the biological hazard of infectious pathogenic microorganisms, also prevent the pollution of culture, also avoided the substratum volatilization.
Embodiment 6 test tubes of the present invention use the process of outside graduated scale participation antibacterials sensitivity Detection as follows
The 1st step was to be that the length of side is 1 centimetre square cylinder with the square body partial design of test tube.
In the 2nd step, test tube guarantees that through sterilization process the test tube body interior of sealing is sterile state.
In the 3rd step, set growth control pipe and drug testing pipe; Open test tube cap, add the fixed amount nutrient solution; Add fixed amount sputum sample, other type sample of needs detection or examined bacterium according to antibacterials sensitivity Detection rules; Add antibacterials in the drug testing pipe; Cover tightly test tube cap, put into suitable culture environment and carry out microbial culture, cultivate detector, manual mycobacterium cultivation detector etc. such as traditional constant incubator, automatic bacterial cultivation detector, manual microbial culture detector, full-automatic mycobacterium.
The 4th step, according to the characteristics of bacterial growth, take out test tube in the specific time, the test tube square body part of observing under inverted microscope detects the specific shape of wherein being examined bacterium, to determine whether the growth of being examined bacterium.
In the 5th step, use one by optical clear material graduated scales preparation, 10 millimeters * 10 millimeters.The square body of test tube of the present invention partly is placed on this graduated scale.Bacterial clone quantity on the scale card of 3 millimeters * 9 millimeters of microscopically countings is measured the height of intraluminal fluid face by graduated scale, calculate the bacterial clone concentration in the respective volume.Can observe the growth control pipe and be examined bacterium to susceptibility and the resistance of this medicine with interior the different of bacterial clone concentration of drug testing pipe with judgement.Need not to uncap and draw bacterium, just can examine under a microscope the growthhabit of bacterium, avoided the biological hazard of infectious pathogenic microorganisms, also prevent the pollution of culture, also avoided the substratum volatilization.
The described structure of above each embodiment, material, step, instrument etc. are signal, or else violate under the prerequisite of inventive concept and spirit, and any modification made for the present invention all should be considered as within protection scope of the present invention.
Claims (10)
1. a Multifunctional test tube that is used for culturing bacterium is characterized in that, this Multifunctional test tube comprises test tube main body and test tube cap; Described test tube main body comprises the connection portion of test tube main body top, test tube lower body part and test tube main body upper and lower; Described test tube lower body part is right cylinder, and described test tube main body top is square body; Described test tube bottom part body is " U " shape or flat.
2. test tube as claimed in claim 1 is characterized in that, the cross section of described test tube main body top square body is square or rectangular; Be carved with on the square body tube wall of described test tube main body top for the scale that calculates bacterial clone quantity, perhaps with being with graduated external accessory to calculate bacterial clone quantity.
3. test tube as claimed in claim 1 or 2 is characterized in that, the cross section of described test tube main body top square body to angular diameter less than or equal to the cylindrical diameter of test tube lower body part.
4. test tube as claimed in claim 1 is characterized in that, the diameter of the connection portion of described test tube main body top and bottom is less than or equal to the maximum diameter of test tube main body top and test tube lower body part.
5. such as claim 1 or 4 described test tubes, it is characterized in that, the connection portion of described test tube main body top and bottom is right cylinder, this cylindrical diameter less than or equal to the cross section on test tube main body top to angular diameter.
6. such as claim 1 or 4 described test tubes, it is characterized in that, the connection portion of described test tube main body top and bottom is irregular body, the connection test tube main body top of this connection portion and the diameter at two ends, bottom are larger, two ends diminish gradually to the diameter of middle portion, and the cross section of this connection portion is the arc of depression.
7. test tube as claimed in claim 1 is characterized in that, described test tube cap is a kind of airtight, ventilative test tube cap, the micron-sized hydrophobic filter membrane of pad in this test tube cap.
8. the using method such as each described Multifunctional test tube for culturing bacterium of claim 1-7 is characterized in that, comprises the steps:
The first step, test tube guarantee that through sterilization process the test tube body interior of sealing is sterile state;
Second step is opened test tube cap, adds nutrient solution, and adding needs the sputum sample that detects, the sample of other type again, covers tightly test tube cap, puts into suitable culture environment and carries out microbial culture;
The 3rd step, according to the characteristics of bacterial growth, take out test tube in the specific time, the direct viewing specific form of bacterium in the liquid nutrient medium in vitro under inverted microscope, determining whether the growth of being examined bacterium, with and growing state.
9. method as claimed in claim 8 is characterized in that, adds following steps before the first step: open test tube cap, add the bacterial growth indicator in the test tube bottom; After the 3rd step, increase following steps: according to the scale on the test tube square body part tube wall, count out the bacterial colony quantity in the scale card; Then calculate bacterial colony concentration according to the bacterial colony quantity in the respective volume.
10. method as claimed in claim 8, it is characterized in that, in the second step, described suitable culture environment comprises that constant incubator, automatic bacterial are cultivated detector, manually microbial culture detector, full-automatic mycobacterium are cultivated detector, manually mycobacterium is cultivated detector.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015150714A1 (en) * | 2014-04-04 | 2015-10-08 | Prestodiag | Method of microbiological analysis of a sample in a unique container |
| CN107034127A (en) * | 2017-06-15 | 2017-08-11 | 黄捷 | A kind of test tube and method for being used to extract microbial gene and Inorganic chemical substance in soil |
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| CN202201900U (en) * | 2011-08-10 | 2012-04-25 | 江西富尔康实业集团有限公司 | Cover type cultivation tube |
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