CN201841020U - Filtering type moulding centrifugal tube for manufacturing cell wax block - Google Patents
Filtering type moulding centrifugal tube for manufacturing cell wax block Download PDFInfo
- Publication number
- CN201841020U CN201841020U CN2010205781777U CN201020578177U CN201841020U CN 201841020 U CN201841020 U CN 201841020U CN 2010205781777 U CN2010205781777 U CN 2010205781777U CN 201020578177 U CN201020578177 U CN 201020578177U CN 201841020 U CN201841020 U CN 201841020U
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Abstract
The utility model discloses a filtering type moulding centrifugal tube for manufacturing a cell wax block. The filtering type moulding centrifugal tube is composed of an upper test tube and a lower test tube, wherein the top end of the upper test tube is opened, and the bottom end of the upper test tube adopts a double-walled structure; the upper test tube is composed of a surface tube wall provided with external screw threads, and an inner tube wall formed by connecting a transition segment and a separation segment; the section of the transition segment decreases from the top down, the bottom part of the transition segment is connected with the top part of the separation segment, and the bottom area of the transition segment is smaller than the sectional area of the top end of the upper test tube; a cell filtering membrane is arranged at the bottom part of the separation segment; the top end of the lower test tube is opened, the inner wall of the lower test tube is provided with the internal screw threads; the internal screw threads are matched with the external screw threads of the surface tube wall at the bottom end of the upper test tube, and the bottom end of the lower test tube is sealed. The two-section filtering type moulding centrifugal tube has centrifuging, filtering and moulding effects; and multiple steps of manufacturing the cell wax block can be finished in one tube. Meanwhile, the filtering type moulding centrifugal tube can realize almost all sampling of the effective organization compositions, is convenient for extracting and can obtains the complete, regular and dimension-adjustable cell wax block.
Description
Technical field
The utility model belongs to the cell pathology field, is specifically related to a kind of moulding centrifuge tube of filtering type that is used to make the cell wax stone.
Background technology
Along with cytopathologic fast development in recent years, take the common smear of conventional cell pathology fabrication techniques, because its limitation has more and more limited cell pathology and used widely at clinical field, mainly show as: there is uniqueness in smear, not reproducible; The residue sample can not forever keep; Borrowing the smear uncertain factor increases; Every emerging pathological technique such as SABC, molecular pathology The Application of Technology have been hindered; The residual tissue structural deterioration is serious, and differing greatly of interference composition in the different samples such as blood, mucus causes the repeatability of cell pathology sample and individuality (doctor, patient) diagnosis difference to increase.
Cell wax stone (cell block, CB) have permanent preservation as sample, repeat advantages such as film-making, institutional framework reservation to greatest extent, antigen and molecular structure fully expose and solved the above-mentioned existing problem of common smear to a great extent, can predict this technology and will be applied in the cell pathology field more and more widely, its making amount is increasing.
(cell block is by toppling over supernatant after the common centrifuge tube centrifugation CB) to traditional cell wax stone, draws then to take out to be deposited on the pipe sediment at the end, again through just completing after a series of programs such as fixing, dehydration, transparent, waxdip, embedding.Its shortcoming is:
(1) centrifugal back " does wet " not separate, and the sediment of supernatant liquid and bottom does not separate, and can not fully blot supernatant liquid and topple over or draw, and can lose the intersection cell component of a part when toppling over imbibition simultaneously yet.
(2) sediment at the bottom of the extraction tube is extremely inconvenient, because test tube elongated (usually greater than 10 centimetres), the sediment of bottom will strip down completely hardly may, sticking of tube wall can be lost relatively large effective cell composition simultaneously, draws also to make the impaired change of portion of tissue particulate broken and make the portion of tissue structure disappear in the process of getting.
(3) wax stone shape, difference in size are remarkable, and the edge is irregular, and piece inner cell composition is loose.
(4) fixing, dehydration, transparent wait process need step by step, be in charge of plural process.
(5) particle of small interference composition such as hemoglobin, fibrin, mucus etc. in the lump
Be deposited in the effective cell composition.
Summary of the invention
The utility model provides a kind of filtering type moulding centrifuge tube, is used for the making of the cell wax stone of cell pathology.
A kind of moulding centrifuge tube of filtering type that is used to make the cell wax stone, constitute by last joint test tube and following joint test tube, wherein, described top of going up the joint test tube is an openend, described bottom of going up the joint test tube is provided with the double-walled construction that is made of top layer tube wall and inner layer tube wall, described top layer tube wall is provided with external screw thread, described inner layer tube wall is formed by connecting by changeover portion and segregation section, the cross section of described changeover portion reduces from top to bottom, the bottom of described changeover portion links to each other with the top of described segregation section, and the bottom surface of described changeover portion is identical with size with the end face shape of described segregation section, the floor space of described changeover portion is less than the described sectional area of going up the top of joint test tube, and the bottom of described segregation section is provided with the cell filtration film; The top of the described test tube of joint down is an openend, and its inwall is provided with internal thread, matches with the described external screw thread of going up the top layer tube wall of joint test tube bottom, and the described bottom of joint test tube down is a sealed end.
In the utility model, described internal thread and external screw thread are waterproof infiltration screw thread.Described upper and lower two joint test tubes are twisted by waterproof infiltration screw thread and are closed connection, when making the cell wax stone, in last joint test tube, collect most active ingredient, save down in the test tube and to store the liquid that filters and unworthy small disturbance composition such as hemoglobin, fibrin, mucus etc., the segregation section that is located at joint test tube bottom will be separated between upper and lower two joint test tubes, be located at cell filtration film bottom the segregation section and be used to carry out separating of effective cell composition and invalid components.
According to actual needs, described upper and lower two save the test tube that test tubes can be chosen different shape and specification, must cooperatively interact by outer, the internal thread that agrees with the top of saving test tube down but go up the bottom of joint test tube.
The length of described segregation section is preferably 0.5～1.0 centimetre by the requirement decision of the required cell wax stone that makes, and with the cell wax stone that acquisition has corresponding height, has in cell pathology the most widely and uses.
Equally, the shape of described segregation section also is by the requirement decision of the required cell wax stone that makes, and according to actual needs, can select different shapes for use, as: cylindrical, square, conical or oval, the pair cell wax stone is moulding.In the optimized technical scheme, described segregation section is cylindrical, can be for being the cylindric of different size, thereby make cell lump condense into to differ in size cylindric.
In order to reach better separating effect, the membrane aperture of described cell filtration film is preferably 5～10 microns, make the plasmid (comprising effective cell component and null cell composition) of centrifugation and liquid by after the filtration of cell filtration film, liquid and interference component see through the cell filtration film and arrive joint test tube down, and the effective cell composition is retained in the joint test tube, thereby realizes separating of " doing wet " separation and active ingredient and interference component.
In order to reach better separating effect, preferred described changeover portion is coaxial with described upward joint test tube.
In order to reach better separation and moulding effect, preferred described segregation section is coaxial with described upward joint test tube.
When adopting the moulding centrifuge tube of filtering type of the present utility model to make the cell wax stone, under the effect of vertical centrifuge, all plasmids that are poured in the liquid in the joint test tube all move down, when the segregation section of the cell filtration film by having certain membrane aperture, nearly all active ingredient is stopped by the cell filtration film and stays, and liquid and micromolecular particle such as hemoglobin, invalid components such as cell fragment then see through the cell filtration film and enter down the joint test tube, reach and do wet the separation, the purpose that active ingredient is separated with invalid components, simultaneously because the inner layer tube wall of last joint test tube bottom is provided with changeover portion is connected with segregation section, under the moulding effect of segregation section, can be made into cell wax stone with unified shape, adopt the segregation section of different size specifications as required, can obtain the cell wax stone of different size specifications.
Adopt the moulding centrifuge tube of filtering type of the present utility model to finish to filter and centrifugal after, can turn on waterproof infiltration screw thread will go up the joint test tube and descend the joint test tube to separate, after discarding useless liquid and interference component, reconnecting continuation uses, fix dehydration, operation such as transparent grade, reach a pipe and promptly finish most of step of making the cell wax stone, for further automation or the semi-automatic cell wax stone of making lay the foundation.Finishing in steps after, turning on waterproof infiltration screw thread will go up the joint test tube and descend the joint test tube to separate, the cell lump that condenses at segregation section directly comes out, can separate columned cell lump by scissors or blade etc. easily, complete take off whole active ingredient after, finish the moulding making of whole cell wax stone.
The moulding centrifuge tube of filtering type of the present utility model, centrifugation and membrane filtration reasonable combination are finished in same test tube, made the effective cell composition separate, when making the cell wax stone with liquid and interference component, can realize almost gross sample of effective structural constituent, and extract conveniently.Further, in the moulding centrifuge tube of same filtering type, can finish centrifugally, filter, aggegation, fixing, dehydration, a plurality of steps such as transparent are for realizing that further the cell wax stone is made automation or semi-automation lays the foundation.
Compared with prior art, the utlity model has following useful technique effect:
Take the binodal test tube, and be provided with double-walled construction in test tube bottom, upper strata, whole test tube has filtration, centrifugal and moulding effect concurrently, thereby realize that the separating of effective cell composition and interference component, sediment separate with liquid, and can extract all effective cell compositions as far as possible, the loss cell amount when reducing routine making cell wax stone; The cell agglutination piece for preparing is moulding complete, regular, and can be with the size of how much adjusting the cell agglutination piece of cell concentration, simultaneously can the whole moulding aggegation cell lump of complete extraction, obtain cell wax stone complete equally, regular, adjustable size; Precipitation, filtration, aggegation, fixing, dehydration, a plurality of loaded down with trivial details steps such as transparent can in vitro be finished same, reduce the artificial step that shifts.。
Description of drawings
Fig. 1 is the structural representation of the moulding centrifuge tube of filtering type of the present utility model.
The specific embodiment
Describe the utility model in detail below in conjunction with embodiment and accompanying drawing, but the utility model is not limited in this.
As shown in Figure 1, a kind of moulding centrifuge tube of filtering type that is used to make the cell wax stone is made of last joint test tube 1 and following joint test tube 2.
The top of last joint test tube 1 is an openend, on save test tube 1 the bottom be provided with the double-walled construction that constitutes by top layer tube wall 3 and inner layer tube wall, top layer tube wall 3 is provided with external screw thread (not shown external screw thread), inner layer tube wall is formed by connecting by changeover portion 4 and segregation section 5.
The cross section of changeover portion 4 reduces from top to bottom, and the bottom of changeover portion 4 links to each other with the top of segregation section 5, and the bottom surface of changeover portion 4 is identical with size with the end face shape of segregation section 5, and the floor space of changeover portion 4 is less than the sectional area on the top of last joint test tube 1; The bottom of segregation section 5 is provided with cell filtration film 6.
The top of saving test tube 2 down is an openend, and the top inwall of following joint test tube 2 is provided with internal thread (not shown internal thread), matches with the external screw thread of the top layer tube wall 3 of the bottom of last joint test tube 1, and the following bottom of saving test tube 2 is a sealed end.
Above-mentioned internal thread and external screw thread are waterproof infiltration screw thread.
Last joint test tube 1 and down the length of joint test tube 2 be 6cm, diameter is 3cm, on the external screw thread segment length that saves on the top layer tube wall 3 of test tube 1 be 1cm, the following internal thread segment length who saves test tube 2 tops is 1cm; Segregation section 5 is cylindrical, and diameter is 1.0cm, highly is 0.5cm, and the bottom is provided with cell filtration film 6, and the membrane aperture of cell filtration film 6 is 5 microns; Changeover portion 4 is up big and down small round table-like, and the bottom surface of changeover portion 4 is identical with size with bottom surface contact and both shapes of segregation section 5; Changeover portion 4, segregation section 5, on save test tube and the joint test tube is all coaxial down.
Segregation section 5 makes the plasmid (comprising effective cell component and null cell composition) of centrifugation and liquid by after the filtration of cell filtration film 6, liquid and interference component see through cell filtration film 6 and arrive joint test tube 2 down, and the effective cell composition is retained in joint test tube 1, thereby realizes separating of " doing wet " separation and active ingredient and interference component.
Last joint test tube 1 and following joint test tube 2 are twisted to close by waterproof infiltration screw thread (internal thread and external screw thread) and are linked together, in last joint test tube 1, collect most active ingredient, save down in the test tube 2 and to store the liquid that filters and unworthy small disturbance composition such as hemoglobin, fibrin, mucus etc., the segregation section 5 that is located at joint test tube 1 bottom will upper and lower two saves test tubes 1 and 2 and separates, and the cell filtration film 6 that is located at segregation section 5 bottoms is used to carry out separating of effective cell composition and invalid components.
When adopting the moulding centrifuge tube of above-mentioned filtering type to make the cell wax stone, under the effect of vertical centrifuge, all plasmids that are poured in the liquid in the joint test tube 1 all move down, by segregation section 5 time, nearly all active ingredient is stopped by cell filtration film 6 and stays, and liquid and micromolecular particle such as hemoglobin, invalid components such as cell fragment then see through cell filtration film 6 and enter down joint test tube 2, reach and do wet the separation, the purpose that active ingredient is separated with invalid components, simultaneously because the inner layer tube wall of last joint test tube 1 bottom is provided with round table-like changeover portion 4 is connected with columned segregation section 5, under the moulding effect of cylindric segregation section 5, can be made into cell wax stone with unified shape, can adopt the cell filtration film post of different size specifications as required, thereby obtain the cell wax stone of different size specifications.
Adopt the above-mentioned moulding centrifuge tube of filtering type to finish to filter and centrifugal after, can turn on waterproof infiltration screw thread will go up joint test tube 1 and descend joint test tube 2 to separate, after discarding useless liquid and interference component, reconnecting continuation uses, fix dehydration, operation such as transparent grade, reach a pipe and promptly finish most of step of making the cell wax stone, for further automation or the semi-automatic cell wax stone of making lay the foundation.Finishing in steps after, turning on waterproof infiltration screw thread will go up joint test tube 1 and descend joint test tube 2 to separate, the cell lump that condenses at segregation section 5 directly comes out, can separate columned cell lump by scissors or blade etc. easily, complete take off whole active ingredient after, finish the moulding making of whole cell wax stone.
The moulding centrifuge tube of above-mentioned filtering type, centrifugation and membrane filtration reasonable combination are finished in same test tube, made the effective cell composition separate, when making the cell wax stone with liquid and interference component, can realize almost gross sample of effective structural constituent, and extract conveniently.Further, in the moulding centrifuge tube of same filtering type, can finish centrifugally, filter, aggegation, fixing, dehydration, a plurality of steps such as transparent are for realizing that further the cell wax stone is made automation or semi-automation lays the foundation.
Claims (7)
1. moulding centrifuge tube of filtering type that is used to make the cell wax stone, it is characterized in that, constitute by last joint test tube and following joint test tube, wherein, described top of going up the joint test tube is an openend, described bottom of going up the joint test tube is provided with the double-walled construction that is made of top layer tube wall and inner layer tube wall, described top layer tube wall is provided with external screw thread, described inner layer tube wall is formed by connecting by changeover portion and segregation section, the cross section of described changeover portion reduces from top to bottom, the bottom of described changeover portion links to each other with the top of described segregation section, and the bottom surface of described changeover portion is identical with size with the end face shape of described segregation section, the floor space of described changeover portion is less than the described sectional area of going up the top of joint test tube, and the bottom of described segregation section is provided with the cell filtration film; The top of the described test tube of joint down is an openend, and its inwall is provided with internal thread, matches with the described external screw thread of going up the top layer tube wall of joint test tube bottom, and the described bottom of joint test tube down is a sealed end.
2. the moulding centrifuge tube of filtering type that is used to make the cell wax stone as claimed in claim 1 is characterized in that, described internal thread and external screw thread are waterproof infiltration screw thread.
3. the moulding centrifuge tube of filtering type that is used to make the cell wax stone as claimed in claim 1 is characterized in that described segregation section is cylindrical.
4. the moulding centrifuge tube of filtering type that is used to make the cell wax stone as claimed in claim 1 is characterized in that the length of described segregation section is 0.5～1.0 centimetre.
5. the moulding centrifuge tube of filtering type that is used to make the cell wax stone as claimed in claim 1 is characterized in that the membrane aperture of described cell filtration film is 5～10 microns.
6. the moulding centrifuge tube of filtering type that is used to make the cell wax stone as claimed in claim 1 is characterized in that, described segregation section is coaxial with described upward joint test tube.
7. the moulding centrifuge tube of filtering type that is used to make the cell wax stone as claimed in claim 1 is characterized in that, described changeover portion is coaxial with described upward joint test tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010205781777U CN201841020U (en) | 2010-10-26 | 2010-10-26 | Filtering type moulding centrifugal tube for manufacturing cell wax block |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010205781777U CN201841020U (en) | 2010-10-26 | 2010-10-26 | Filtering type moulding centrifugal tube for manufacturing cell wax block |
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| CN201841020U true CN201841020U (en) | 2011-05-25 |
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| CN2010205781777U Expired - Fee Related CN201841020U (en) | 2010-10-26 | 2010-10-26 | Filtering type moulding centrifugal tube for manufacturing cell wax block |
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| CN102539214A (en) * | 2012-02-23 | 2012-07-04 | 杭州遂真生物技术有限公司 | Sample-processing combined test tube and sample-processing method |
| CN102899242A (en) * | 2012-11-02 | 2013-01-30 | 中华人民共和国上海出入境检验检疫局 | Multifunctional test tube for cultivating bacteria and using method of multifunctional test tube |
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| CN103954486A (en) * | 2014-04-23 | 2014-07-30 | 杭州电子科技大学 | Pressurized reaction device for preparing cell wax block |
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2010
- 2010-10-26 CN CN2010205781777U patent/CN201841020U/en not_active Expired - Fee Related
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| CN102539214A (en) * | 2012-02-23 | 2012-07-04 | 杭州遂真生物技术有限公司 | Sample-processing combined test tube and sample-processing method |
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| CN103543057B (en) * | 2013-11-13 | 2015-12-02 | 何鑫 | The Combined centrifuge tube of cytology wax stone is prepared for micro-cast-off cells |
| CN103543057A (en) * | 2013-11-13 | 2014-01-29 | 何洪敏 | Combined type centrifuge tube for preparation of cytological paraffin block by using trace cast-off cells |
| CN105928757A (en) * | 2014-04-23 | 2016-09-07 | 杭州电子科技大学 | Automatic paraffin cell block preparation system |
| CN105628477B (en) * | 2014-04-23 | 2018-01-16 | 杭州电子科技大学 | The full-automatic device for quickly preparing cell block |
| CN103969096A (en) * | 2014-04-23 | 2014-08-06 | 杭州电子科技大学 | Automatic cell paraffin block preparation device and method |
| CN105628477A (en) * | 2014-04-23 | 2016-06-01 | 杭州电子科技大学 | Device for automatically and quickly preparing cell wax block |
| CN105675373A (en) * | 2014-04-23 | 2016-06-15 | 杭州电子科技大学 | Cell paraffin block preparation device with quick locating function |
| CN105699153A (en) * | 2014-04-23 | 2016-06-22 | 杭州电子科技大学 | Automatic cell paraffin block making equipment with protection function |
| CN105865864B (en) * | 2014-04-23 | 2019-01-25 | 杭州电子科技大学 | Cell block processing unit (plant) with quick preparation function |
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| CN103954486A (en) * | 2014-04-23 | 2014-07-30 | 杭州电子科技大学 | Pressurized reaction device for preparing cell wax block |
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| CN105675373B (en) * | 2014-04-23 | 2018-11-09 | 杭州电子科技大学 | Cell block preparation facilities with fast positioning function |
| CN106140494A (en) * | 2015-04-02 | 2016-11-23 | 马铁军 | A kind of centrifugal when use special bottom opening centrifuge tube device |
| CN104801089A (en) * | 2015-05-07 | 2015-07-29 | 江苏衡昇仪器有限公司 | Liquid extraction test tube |
| CN105817346A (en) * | 2016-01-25 | 2016-08-03 | 天津城建大学 | Solid-liquid separation device used for soil body |
| CN106000502A (en) * | 2016-07-06 | 2016-10-12 | 江苏科华医疗器械科技有限公司 | Ultrafiltration centrifugation tube with protective jacket |
| CN113440927A (en) * | 2020-03-27 | 2021-09-28 | 张文杰 | Device and method for extracting water-soluble components of emulsified and broom-corn-like fat |
| CN113440927B (en) * | 2020-03-27 | 2022-05-20 | 张文杰 | Device and method for extracting water-soluble components of emulsified and broom corn fat |
| CN113390692A (en) * | 2021-05-24 | 2021-09-14 | 杭州电子科技大学 | Centrifugal test tube for automatically preparing cell wax block |
| CN116106108A (en) * | 2023-02-13 | 2023-05-12 | 杭州瑞普晨创科技有限公司 | Cell wax block and embedding method for frozen sections of small number of cells |
| CN116106108B (en) * | 2023-02-13 | 2024-04-05 | 杭州瑞普晨创科技有限公司 | Cell wax block and embedding method for frozen sections of small number of cells |
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110525 Termination date: 20161026 |