CN103543057A - Combined type centrifuge tube for preparation of cytological paraffin block by using trace cast-off cells - Google Patents
Combined type centrifuge tube for preparation of cytological paraffin block by using trace cast-off cells Download PDFInfo
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- CN103543057A CN103543057A CN201310569818.0A CN201310569818A CN103543057A CN 103543057 A CN103543057 A CN 103543057A CN 201310569818 A CN201310569818 A CN 201310569818A CN 103543057 A CN103543057 A CN 103543057A
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Abstract
The invention provides a combined type centrifuge tube used for preparation of cytological paraffin block by using trace cast-off cells (less than 1-2 cubic millimeters or below by cell volume). According to the technical scheme, the combined type centrifuge tube for preparation of cytological paraffin block by using trace cast-off cells comprises a special dehydration embedding box and is characterized by further comprising a top tube, a cell mass collecting forming tube and a bottom tube which are all made of transparent material, wherein the top tube is a through tube, the inner and outer diameters of one end of the top tube are closed at the same time and the outer diameter of the top tube is provided with a thread. The combined type centrifuge tube can be operated simply and can completely collect trace cytological samples generated by puncture and the like in suspension liquid and centrifuge the collected samples so that solid phase components are endowed with a standard mode, thereby providing a material for the follow-up work and improving the quality of medical diagnosis.
Description
Technical field
The present invention is a kind of Combined centrifuge tube, prepares cytology wax stone use for the medical pathologies micro-cast-off cells of specialty (1-2 cubic millimeter or following cell body accumulated amount).
background technology:
Exfoliative cytology inspection is the important component part of medical pathologies and medical test, the general smear of variety of way that adopts is as the Main Means checking, according to need of work, can, after completing smear making, for retaining remaining cell component, be prepared into cytology wax stone, in real work, few cells is learned sample and is being prepared particularly difficulty of cytology wax stone process, and poor effect, is difficult to meet need of work.
And at present, medical institutions' few cells is learned wax stone and is made the methods such as centrifugal rear dropping serum, albumen, agar that mostly are, then manual operations is agglomerated together by broken Cell Component, adopt after filter paper parcel, Dehydration is for cytology, cannot make made cell lump keep having certain constant volume shape, inefficiency, made wax stone quality is unstable.
Summary of the invention
The object of the present invention is to provide and a kind ofly for micro-cast-off cells, prepare the Combined centrifuge tube that cytology wax stone is used, solved the problem preparation of few cells composition in real work to the cell lump of certain standardization proterties.
The technical scheme that this patent adopts is: a kind ofly for micro-cast-off cells, prepare the Combined centrifuge tube that cytology wax stone is used, comprise special-purpose embedding box for dehydrating, it is characterized in that, also comprise that the push pipe of transparent material, cell lump collect excipient pipe, bottom tube;
Described push pipe is siphunculus, and wherein push pipe one end internal-and external diameter closes up simultaneously, and external diameter is provided with screw thread;
It is that fixed head forms with the embedded or circumscribed excipient pipe being firmly connected with fixed head that described cell lump is collected excipient pipe, and the internal-and external diameter of fixed head is identical with push pipe closing in end internal-and external diameter, and fixed head is also provided with symmetrical clamp through hole;
Described bottom tube is semicircle chondritic, its disc is provided with inner tube, described inner tube is top and the bottom, top is divided into large-diameter pipe, bottom is divided into small diameter tube, large-diameter pipe internal diameter is identical with cell lump collection excipient pipe external diameter, and small diameter tube is identical with cell lump collection excipient bore, and in large footpath, tube portion is provided with the screw thread that closes up and hold screw thread to match with push pipe;
Described special-purpose embedding box for dehydrating middle part is provided with excipient pipe embedding casting hole, in inner side, special-purpose embedding box for dehydrating bottom surface, is respectively equipped with a pair of arc piece deck identical with cell lump collection excipient pipe outside dimension with the putting hole periphery in outside.
According to described, for micro-cast-off cells, prepare the Combined centrifuge tube that cytology wax stone is used, it is characterized in that, described excipient pipe is for to make with plastic sheeting or the biomaterial built up membrane of micropore.
The advantage of this patent is: simple to operate, and the few cells such as the puncture in suspending liquid can be learned to sample and all collect, and after centrifugal, accomplish to give solid phase composition and there is certain canonical schema, for follow-up work provides material, improve medical diagnosis quality.
accompanying drawing explanation:
Fig. 1 is explosive view of the present invention.
Fig. 2 is assembled sectional view of the present invention.
Fig. 3 is special-purpose embedding box for dehydrating structural drawing of the present invention.
Fig. 4 is cell lump of the present invention schematic diagram when collecting excipient pipe and being placed on embedding casting inside special-purpose embedding box for dehydrating.
Fig. 5 is cell lump of the present invention schematic diagram when collecting excipient pipe and being placed on the outer side slicing of special-purpose embedding box for dehydrating.
Legend: 1, push pipe, 2, cell lump collects excipient pipe, 3, clamp through hole, 4, excipient pipe, 5, bottom tube, 6, special-purpose embedding box for dehydrating, 7, excipient pipe embedding casting hole, 8, arc piece deck, 9, inner tube.
Embodiment
Below in conjunction with the drawings and specific embodiments, the invention will be further described:
The present invention as shown in the figure,
For micro-cast-off cells, prepare the Combined centrifuge tube that cytology wax stone is used, comprise special-purpose embedding box for dehydrating 6, it is characterized in that, also comprise push pipe 4, cell lump collection excipient pipe 2, the bottom tube 5 of transparent material;
Described push pipe 4 is siphunculus, and wherein push pipe one end internal-and external diameter closes up simultaneously, and external diameter is provided with screw thread;
Described cell lump is collected excipient pipe 4 compositions that excipient pipe 2 is fixed head and fixed head internal diameter same diameter, and the internal-and external diameter of fixed head is identical with push pipe closing in end internal-and external diameter, and fixed head is also provided with symmetrical clamp through hole 3;
Described bottom tube 5 is semicircle chondritic, its disc is provided with inner tube 9, described inner tube is top and the bottom, top is divided into large-diameter pipe, bottom is divided into small diameter tube, large-diameter pipe internal diameter is identical with cell lump collection excipient pipe 2 external diameters, and small diameter tube is identical with cell lump collection excipient pipe 2 internal diameters, and in large footpath, tube portion is provided with the screw thread that closes up and hold screw thread to match with push pipe 1;
Described special-purpose embedding box for dehydrating middle part is provided with excipient pipe embedding casting hole 7, in special-purpose embedding box for dehydrating 6 inner sides, bottom surface, is respectively equipped with a pair of arc piece deck 8 identical with cell lump collection excipient pipe 2 outside dimensions with the putting hole periphery in outside.
According to described, for micro-cast-off cells, prepare the Combined centrifuge tube that cytology wax stone is used, it is characterized in that, described excipient pipe 4 is for to make with plastic sheeting or the biomaterial built up membrane of micropore.
As Fig. 1 push pipe and bottom tube are threaded (or draw-in groove), after spinning, make that push pipe, cell lump are collected excipient pipe, bottom tube forms a Unified Global.
Cell lump is collected excipient pipe 2 and is connected with push pipe 1 formation contact, cell lump is collected the fixed head of excipient pipe 2 except the hole corresponding with push pipe bottom, center, separately offer 2 above perforates, convenient operation person uses tweezers clamping mobile, excipient pipe 4 forms and (requires and cell lump embedded section together by biomaterial or with aperture plastic sheeting material, do not affect chipping qualities), excipient pipe 4 can not be separated with firm connection of fixed head.Bottom tube 5 base seals, internal volume and figuration pipe 4 match, and top is with the screw thread (or draw-in groove) matching with push pipe bottom thread (or draw-in groove); Push pipe is collected between excipient pipe fixed head and bottom tube push pipe bottom, cell lump after relying on screw thread (or draw-in groove) to be connected with bottom tube and is produced certain pressure, and makes whole tube wall have certain antiseepage leakproofness.When push pipe is separated with bottom tube, use tweezers clamping to collect excipient pipe top tie plate, after taking-up, enter preparation flow.
Special-purpose embedding box for dehydrating: center is opened one and collected the consistent perforate in excipient pore footpath with cell lump, perforate both sides have with cell lump collects the arc piece deck that excipient pipe fixed head matches, in conjunction with time to collect excipient pipe 2 position of upper consistent with cell lump, while being convenient to embedding to the interior casting of figuration pipe 4, two-sided all have with cell lump collect the arc piece deck that excipient pipe fixed head mates.
Use operation steps: this centrifuge tube is that medical pathologies specialty is prepared the use of cytology wax stone for micro-cast-off cells (1-2 cubic millimeter or following cell body accumulated amount), during use, the cell suspension of suitable dosage (being generally once centrifugal rear concentrated cell suspension) is injected to push pipe, centrifuge tube is put into horizontal type centrifuger centrifugal, centrifugal rear cell component is deposited on centrifuge tube bottom cell lump and collects in excipient pipe, taking out centrifuge tube inclines to after supernatant, now, solid phase composition in cell suspension is deposited on cell lump and collects in excipient pipe, and the formation shape identical with excipient bore, after separated push pipe and bottom tube, with tweezers clamping cell lump, collect figuration pipe top tie plate, with routine pathology organization embedding dedicated paper, cover upper opening, the fixed head that cell lump is collected to figuration pipe 2 embed special-purpose embedded box arc piece deck fixing after, cover embedding lid, put into neutral formalin immobile liquid fixedly after 1-2 hour, can enter conventional dehydration, waxdip program.During embedding, cell lump being collected to the fixed head embedding embedded box back side outer bayonet of figuration pipe fixes, together with cell lump, collect figuration pipe 2 and together put into the embedding of tissue block imbedded mold, adopt the figuration pipe of special substance together to cut into slices with cell lump, do not affect chipping qualities.
Claims (2)
1. for micro-cast-off cells, prepare the Combined centrifuge tube that cytology wax stone is used, comprise special-purpose embedding box for dehydrating (6), it is characterized in that, also comprise push pipe (4), cell lump collection excipient pipe (2), the bottom tube (5) of transparent material;
Described push pipe (4) is siphunculus, and wherein push pipe one end internal-and external diameter closes up simultaneously, and external diameter is provided with screw thread;
Described cell lump is collected excipient pipe (2) and is formed for fixed head and the embedded or circumscribed excipient pipe (4) that is fixedly connected with fixed head, and the internal-and external diameter of fixed head closes up and hold internal-and external diameter identical with push pipe, and fixed head is also provided with symmetrical clamp through hole (3);
Described bottom tube (5) is semicircle chondritic, its disc is provided with inner tube (9), described inner tube is top and the bottom, top is divided into large-diameter pipe, bottom is divided into small diameter tube, large-diameter pipe internal diameter is identical with cell lump collection excipient pipe (2) external diameter, and small diameter tube is identical with cell lump collection excipient pipe (2) internal diameter, and in large footpath, tube portion is provided with the screw thread that closes up and hold screw thread to match with push pipe (1);
Described special-purpose embedding box for dehydrating middle part is provided with excipient pipe embedding casting hole (7), surveys with the putting hole periphery in outside and be respectively equipped with a pair of arc piece deck (8) identical with cell lump collection excipient pipe (2) outside dimension in special-purpose embedding box for dehydrating (6) bottom surface.
2. according to claim 1ly for micro-cast-off cells, prepare the Combined centrifuge tube that cytology wax stone is used, it is characterized in that, described excipient pipe (4) is for to make with plastic sheeting or the biomaterial built up membrane of micropore.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310569818.0A CN103543057B (en) | 2013-11-13 | 2013-11-13 | The Combined centrifuge tube of cytology wax stone is prepared for micro-cast-off cells |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310569818.0A CN103543057B (en) | 2013-11-13 | 2013-11-13 | The Combined centrifuge tube of cytology wax stone is prepared for micro-cast-off cells |
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| Publication Number | Publication Date |
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| CN103543057A true CN103543057A (en) | 2014-01-29 |
| CN103543057B CN103543057B (en) | 2015-12-02 |
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| CN201310569818.0A Expired - Fee Related CN103543057B (en) | 2013-11-13 | 2013-11-13 | The Combined centrifuge tube of cytology wax stone is prepared for micro-cast-off cells |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103954486A (en) * | 2014-04-23 | 2014-07-30 | 杭州电子科技大学 | Pressurized reaction device for preparing cell wax block |
| CN104359746A (en) * | 2014-11-19 | 2015-02-18 | 白银市第一人民医院 | Cell mass collector and method for collecting cells in liquid specimen |
| CN105628477A (en) * | 2014-04-23 | 2016-06-01 | 杭州电子科技大学 | Device for automatically and quickly preparing cell wax block |
| CN105675373A (en) * | 2014-04-23 | 2016-06-15 | 杭州电子科技大学 | Cell paraffin block preparation device with quick locating function |
| CN106979881A (en) * | 2017-05-18 | 2017-07-25 | 桂林医学院 | A kind of centrifuge tube prepared for cell block |
| CN111811901A (en) * | 2020-06-30 | 2020-10-23 | 郑志森 | Cell block collecting device and cell block collecting method |
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| CN202191476U (en) * | 2011-08-11 | 2012-04-18 | 杭州格莱迈科技有限公司 | Centrifuge tube |
| CN203616181U (en) * | 2013-11-13 | 2014-05-28 | 何鑫 | Combined type centrifuge tube for preparing cytological wax block with trace of exfoliative cells |
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| CN1661039A (en) * | 2004-12-06 | 2005-08-31 | 李文生 | Method for preparing chips of cell and equipment |
| CN201825948U (en) * | 2010-09-30 | 2011-05-11 | 何洪敏 | Combined centrifuge tube for preparing cell blocks by using cast-off cells |
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105928757A (en) * | 2014-04-23 | 2016-09-07 | 杭州电子科技大学 | Automatic paraffin cell block preparation system |
| CN105628477B (en) * | 2014-04-23 | 2018-01-16 | 杭州电子科技大学 | The full-automatic device for quickly preparing cell block |
| CN105628477A (en) * | 2014-04-23 | 2016-06-01 | 杭州电子科技大学 | Device for automatically and quickly preparing cell wax block |
| CN105675373A (en) * | 2014-04-23 | 2016-06-15 | 杭州电子科技大学 | Cell paraffin block preparation device with quick locating function |
| CN105842028A (en) * | 2014-04-23 | 2016-08-10 | 杭州电子科技大学 | Cell paraffin block preparation device with pressurization and extraction functions |
| CN105890944A (en) * | 2014-04-23 | 2016-08-24 | 杭州电子科技大学 | Cell paraffin block preparing device with mechanical arm |
| CN105842028B (en) * | 2014-04-23 | 2019-02-22 | 杭州电子科技大学 | Cell block preparation facilities with pressurization, extract function |
| CN105890944B (en) * | 2014-04-23 | 2019-01-25 | 杭州电子科技大学 | Cell block preparation facilities with mechanical arm |
| CN103954486A (en) * | 2014-04-23 | 2014-07-30 | 杭州电子科技大学 | Pressurized reaction device for preparing cell wax block |
| CN105675373B (en) * | 2014-04-23 | 2018-11-09 | 杭州电子科技大学 | Cell block preparation facilities with fast positioning function |
| CN105928757B (en) * | 2014-04-23 | 2019-01-25 | 杭州电子科技大学 | Self-action cell block preparation system |
| CN104359746A (en) * | 2014-11-19 | 2015-02-18 | 白银市第一人民医院 | Cell mass collector and method for collecting cells in liquid specimen |
| CN106979881A (en) * | 2017-05-18 | 2017-07-25 | 桂林医学院 | A kind of centrifuge tube prepared for cell block |
| CN111811901A (en) * | 2020-06-30 | 2020-10-23 | 郑志森 | Cell block collecting device and cell block collecting method |
| CN111811901B (en) * | 2020-06-30 | 2022-09-20 | 郑志森 | Cell block collecting device and cell block collecting method |
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Owner name: HE XIN Free format text: FORMER OWNER: HE HONGMIN Effective date: 20140320 |
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| C41 | Transfer of patent application or patent right or utility model | ||
| C53 | Correction of patent of invention or patent application | ||
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Inventor after: He Xin Inventor before: He Hongmin Inventor before: Song Jilan Inventor before: He Xin |
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Free format text: CORRECT: ADDRESS; FROM: 277100 ZAOZHUANG, SHANDONG PROVINCE TO: 271016 TAIAN, SHANDONG PROVINCE |
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Effective date of registration: 20140320 Address after: 271016 medical imaging (Chinese foreign cooperative education), School of Radiology, 13 new campus of Taishan Medical University, 619 the Great Wall Road, Shandong, Tai'an Applicant after: He Xin Address before: 277100 Department of Pathology, Zaozhuang municipal hospital, 47 leading Road, Shizhong District, Zaozhuang, Shandong Applicant before: He Hongmin |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20151202 Termination date: 20161113 |