CN207703577U - Disposable cell column forming devices - Google Patents
Disposable cell column forming devices Download PDFInfo
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- CN207703577U CN207703577U CN201820078462.9U CN201820078462U CN207703577U CN 207703577 U CN207703577 U CN 207703577U CN 201820078462 U CN201820078462 U CN 201820078462U CN 207703577 U CN207703577 U CN 207703577U
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- Prior art keywords
- cell column
- lower cavity
- forming devices
- push
- pin
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Abstract
The utility model is related to a kind of disposable cell column forming devices, including：Shaping conduit, upper cover, bottom cover and push-off pin；The shaping conduit includes：Upper chamber and lower cavity, the lower end of upper chamber and the upper end of lower cavity connect；The upper end of the upper chamber is equipped with upper orifice, and upper orifice is connected with upper cover；The lower end of the lower cavity is equipped with lower nozzle, and lower nozzle is connected with bottom cover；The push-off pin enters along shaping conduit central axes from upper orifice just by lower cavity, and the cell column after centrifugal forming is released.
Description
Technical field
The utility model is related to field of medical device, are a kind of cell collection devices, specifically disposable cell column
Forming devices.
Background technology
In RESEARCH ON CELL-BIOLOGY and cell pathology technical field, it will usually cell sample are fabricated to agglomerate, so
Paraffin embedding is used afterwards so that cell is no longer directly exposed in air or fixer, and cell is preserved so as to the long period
Interior component is the common cell sample store method in laboratory.
Existing cell mass is collected by the way of the collection of standard cone bottom centrifuge tube, the specific steps are：By cell
Suspension is placed in centrifuge tube, through high speed centrifugation, after discarding supernatant, carefully knocks out and collects the cell mass after concentration.
The method that existing standard cone bottom centrifuge tube collects cell mass, there are some defects, and being unfavorable for collection standard can
The cell mass of control：
1) since standard cone bottom centrifugation bottom of the tube is taper, the cell mass after collecting is cone so that cell mass
Cross section of the block in cone center axis direction is inconsistent, and the cell sample constituent content contained by every paraffin section is caused to differ
It causes, is unfavorable for normalized analysis；
2) it abandons after supernatant, being located at the cell mass at centrifuge tube bottom is inconvenient to take out, and is easily damaged during taking out
Cell mass.
Due to existing technical method, the cell mass cross sectional dimensions of acquisition is inhomogenous, and collects easy pair in the process
Cell mass damages, and influences the consistency of subsequent cell composition analysis result.In view of above-mentioned objective insufficient and disadvantage, if
The utility model is counted.Concretely the purpose of this utility model is to provide a kind of disposable cell column forming devices,
This cell column forming devices can be used for centrifuge cell suspension by one and cell settlement made to form the shaping conduit of cell column, a use
Upper cover, a bottom cover and a release for releasing moulding cell column for sealing shaping conduit in sealing shaping conduit
Bar forms.Bottom cover is covered, the shaping conduit, then top cover is added in cell suspension, after supernatant is abandoned in centrifugation, will be centrifuged with push-off pin
The cell column that deposition is formed is released, and the collection of cell mass is completed.Complete cell column is can get, and can be to the diameter of cell column
(cross section) is standardized control.Cell mass is made using the utility model, there is standardization level height, to cell mass
Small feature is damaged, objective can meet the needs of scientific research and Clinical practice person making high quality standards cell paraffin section.
After agglomerate completes, paraffin section can be done, for the component analysis in cell sample.The quality of chipping qualities is straight
It connects and influences precision of analysis, especially do quantitative analysis.It is therefore desirable to obtain, dimension criteria is controllable, consistency is strong
And few high quality cell mass is damaged to cell sample.
Utility model content
The utility model devises disposable cell column forming devices, overcome or substantially alleviate with it is existing
The related above problem of technology and/or other problems.
To achieve the above objectives, the technical scheme adopted by the utility model is that：
Disposable cell column forming devices, including：Shaping conduit 1, upper cover 2, bottom cover 3 and push-off pin 4；
The shaping conduit 1 includes：Upper chamber 5 and lower cavity 6, the upper end of the lower end and lower cavity 6 of upper chamber 5
Connection；
The upper end of the upper chamber 5 is equipped with upper orifice 7, and upper orifice 7 is connected with upper cover 2；
The lower end of the lower cavity 6 is equipped with lower nozzle 8, and lower nozzle 8 is connected with bottom cover 3；
The push-off pin 4 enters along 1 central axes of shaping conduit from upper orifice 7 just by lower cavity 6, will be centrifuged into
Cell column after type is released.
On the basis of said program, external screw thread is equipped at the upper orifice 7 and lower nozzle 8.
On the basis of said program, the inside of the upper cover 2 and bottom cover 3 is equipped with internal thread, respectively with upper orifice 7
The external screw thread at place and the external screw thread at lower nozzle 8 are fitted close.
On the basis of said program, the shaping conduit 1 is used to store the supernatant of the mixing with cells suspension after centrifugation,
After centrifugation, up along lower nozzle 8, cell column is formed, cell column length does not exceed the length of lower cavity 6 in principle.
On the basis of said program, the push-off pin 4 includes：The body of rod, the diameter of the body of rod are slightly less than the pipe of lower cavity 6
Internal diameter.
On the basis of said program, the push-off pin 4 includes：It releases club shaft 4.1 and releases club head 4.2, it is described
The diameter of club head 4.2 is released slightly larger than the diameter for releasing club shaft 4.1, the diameter for releasing club shaft 4.1 is slightly less than lower chamber
The bore of body 6 facilitates and releases the cell column after centrifugal forming.
The utility model can be matched to be made in cell sample paraffin section, realize standard it is controllable, to cell sample
Damage the collection of few cell sample agglomerate.
Compared with existing device, the technical characterstic of the utility model has：
1, column tube bottom is designed, and be can be used for centrifugation and is formed the controllable cell column agglomerate of dimensional standard；
2, logical bottom centrifuge tube and the push-off pin design of threaded block, convenient for the ejection of cell mass after the completion of centrifugation；
3, purifying cells can be efficiently separated and the damage very little to cell；
4, unique centrifugation fixing device, the molding of convenience sample and subsequent processing；
5, the design of the separable lid in both ends, molding cell mass of being more convenient for take out, and improve experiment success rate and experiment is imitated
Rate.
Description of the drawings
The utility model has following attached drawing：
Fig. 1 is the part-structure schematic diagram of disposable cell column forming devices；
Fig. 2 is the structure enlarged view of shaping conduit in Fig. 1；
Fig. 3 is the structural schematic diagram of push-off pin
Fig. 4 is push-off pin another kind structural schematic diagram；
Attached figure label explanation：
1, shaping conduit, 2, upper cover, 3, bottom cover, 4, push-off pin, 4.1 release club shafts, and 4.2, release head, 5, upper chambers
Body, 6, lower cavity, 7, upper orifice, 8, lower nozzle.
Specific implementation mode
The utility model is described in further detail below in conjunction with attached drawing 1-3.
The utility model provides a kind of disposable cell column forming devices, including：Shaping conduit 1, upper cover 2, bottom cover 3
And push-off pin 4；
The shaping conduit 1 includes：Upper chamber 5 and lower cavity 6, the lower end of upper chamber 5 are upper with lower cavity 6
End connection；
The upper end of the upper chamber 5 is equipped with upper orifice 7, and upper orifice 7 is connected with upper cover 2；
The lower end of the lower cavity 6 is equipped with lower nozzle 8, and lower nozzle 8 is connected with bottom cover 3；
The push-off pin 4 enters along 1 central axes of shaping conduit from upper orifice 7 just by lower cavity 6, will centrifuge
Cell column after molding is released.
On the basis of said program, external screw thread is equipped at the upper orifice 7 and lower nozzle 8.
On the basis of said program, the inside of the upper cover 2 and bottom cover 3 is equipped with internal thread, respectively with upper orifice 7
The external screw thread at place and the external screw thread at lower nozzle 8 are fitted close.
On the basis of said program, the shaping conduit 1 is used to store the supernatant of the mixing with cells suspension after centrifugation,
After centrifugation, up along lower nozzle 8, cell column is formed, cell column length does not exceed the length of lower cavity 6 in principle.
On the basis of said program, the push-off pin 4 includes：Club shaft 4.1 is released, the straight of club shaft 4.1 is released
Diameter is slightly less than the bore of lower cavity 6.
On the basis of said program, the push-off pin 4 includes：It releases club shaft 4.1 and releases club head 4.2, institute
The diameter for releasing club head 4.2 is stated slightly larger than the diameter for releasing club shaft 4.1, the diameter for releasing club shaft 4.1 is slightly less than lower part
The bore of cavity 6 facilitates and releases the cell column after centrifugal forming.
Embodiment
Disposable cell column forming devices, including：1 long 15cm of shaping conduit；2 interior diameter 1.5cm of upper cover, overall diameter
1.7cm, high 2cm；3 interior diameter 0.5cm of bottom cover, overall diameter 0.7cm, high 2cm；Push-off pin 4 long 20cm, shank diameter 0.5cm；
The shaping conduit 1 is made of upper chamber 5 and lower cavity 6,5 long 9cm of upper chamber, 6 long 6cm of lower cavity；
5 diameter 1.5cm of the upper chamber, upper end have upper orifice 7 that can be connected with upper cover 2；
There is 8 diameter 0.5cm of lower nozzle in 6 lower end of the lower cavity, can be connected with bottom cover 3；
The push-off pin 4 passes through lower cavity 6 just.
On the basis of said program, it is equipped with outside 3 circles at the upper chamber 5 of the shaping conduit 1 and the end of lower cavity 6
Screw thread.
On the basis of said program, there is 2 circle internal threads in the upper cover 2 and bottom cover 3, can respectively with upper orifice 7
External screw thread and the external screw thread of lower nozzle 8 are fitted close.
On the basis of said program, the shaping conduit 1 can store the supernatant of the mixing with cells suspension after centrifugation, from
After the heart, up along lower nozzle 8, cell column is formed, cell column length does not exceed the length 6cm of lower cavity 6 in principle.
When use, bottom cover 3 is screwed at the lower nozzle 8 of lower cavity 6, is tightened；Then cell to be separated is mixed
Suspension is packed into shaping conduit 1 from upper orifice 7, and upper cover 2 is then screwed in upper orifice 7, is tightened；It is then placed within inside centrifuge
It is centrifuged, take 10-15ml as primary, 1000rpm/min revolutions, centrifuged 5 minutes.Centrifugation 35 times by several times, first 33 times according to every
Pipe centrifuges the volume of 10-15ml, and the volume of the 34th centrifugation 5ml, the 35th time, the cell mass obtained after first 34 times are centrifuged is again
It is blended in inside the 35th centrifuge tube, cell suspending liquid is added, after mixing well, is centrifuged, turned with 1000rpm/min
Speed centrifuges 5 minutes.
After five minutes, stop centrifugation, unscrew upper cover 2, centrifuged supernatant is poured out from upper orifice 7；Bottom cover 3 is unscrewed, with pushing away
Rod 4 slowly releases molding cell cylinder from upper orifice 7 along 1 central axes of shaping conduit, carries out at corresponding subsequent experimental
Reason.
When the push-off pin 4 is second of structure, the pipe that 4.2 diameter 0.49cm of club head is slightly less than lower cavity 6 is released
Internal diameter 0.5cm releases 4.1 diameter 0.4cm of club shaft.
The utility model is sealing during implementation, prevents leakage of the sample in centrifugal process.
It is described above simply to illustrate that the utility model principle.Further, since this utility model operating technology
Dexterity easily causes the utility model and generates many changes, and any one skilled in the art is new in this practicality
In the technical scope that type discloses, the change or replacement that can be readily occurred in should be covered within the scope of the utility model.
The content not being described in detail in this specification belongs to the prior art well known to professional and technical personnel in the field.
Claims (6)
1. disposable cell column forming devices, which is characterized in that including：Shaping conduit (1), upper cover (2), bottom cover (3) and
Push-off pin (4)；
The shaping conduit (1) includes：Upper chamber (5) and lower cavity (6), lower end and the lower cavity (6) of upper chamber (5)
Upper end connection；
The upper end of the upper chamber (5) is equipped with upper orifice (7), and upper orifice (7) is connected with upper cover (2)；
The lower end of the lower cavity (6) is equipped with lower nozzle (8), and lower nozzle (8) is connected with bottom cover (3)；
The push-off pin (4) enters along shaping conduit (1) central axes from upper orifice (7) just by lower cavity (6), will be from
Heart cell column after molding is released.
2. disposable cell column forming devices as described in claim 1, which is characterized in that the upper orifice (7) and
Lower nozzle is equipped with external screw thread at (8).
3. disposable cell column forming devices as claimed in claim 2, which is characterized in that the upper cover (2) and bottom cover
(3) internal thread is equipped on the inside of, it is close with the external screw thread at upper orifice (7) and the external screw thread at lower nozzle (8) respectively
Cooperation.
4. disposable cell column forming devices as described in claim 1, which is characterized in that the push-off pin (4) includes：
The body of rod, the diameter of the body of rod are slightly less than the bore of lower cavity (6).
5. disposable cell column forming devices as described in claim 1, which is characterized in that the push-off pin (4) includes：
It releases club shaft (4.1) and releases club head (4.2), the diameter for releasing club head (4.2) is slightly larger than release club shaft
(4.1) diameter, the diameter for releasing club shaft (4.1) are slightly less than the bore of lower cavity (6), and facilitating will be after centrifugal forming
Cell column is released.
6. disposable cell column forming devices as described in claim 1, which is characterized in that the shaping conduit (1) is used for
The supernatant for storing the mixing with cells suspension after centrifugation after centrifugation, up along lower nozzle (8), forms cell column, cell column
The length of of length no more than lower cavity (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820078462.9U CN207703577U (en) | 2018-01-18 | 2018-01-18 | Disposable cell column forming devices |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820078462.9U CN207703577U (en) | 2018-01-18 | 2018-01-18 | Disposable cell column forming devices |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207703577U true CN207703577U (en) | 2018-08-07 |
Family
ID=63035219
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820078462.9U Active CN207703577U (en) | 2018-01-18 | 2018-01-18 | Disposable cell column forming devices |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207703577U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111811901A (en) * | 2020-06-30 | 2020-10-23 | 郑志森 | Cell block collecting device and cell block collecting method |
-
2018
- 2018-01-18 CN CN201820078462.9U patent/CN207703577U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111811901A (en) * | 2020-06-30 | 2020-10-23 | 郑志森 | Cell block collecting device and cell block collecting method |
| CN111811901B (en) * | 2020-06-30 | 2022-09-20 | 郑志森 | Cell block collecting device and cell block collecting method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210209 Address after: 100176 Room 401, 4th floor, building 11, 156 Jinghai 4th Road, Beijing Economic and Technological Development Zone, Daxing District, Beijing Patentee after: BEIJING SEARCH BIOTECH Co.,Ltd. Address before: 100176 Beijing Daxing District Beijing economic and Technological Development Zone No. four, 156 14, Jinghai four road. Patentee before: BEIJING GENOMEPRECISION TECHNOLOGY Co.,Ltd. |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20230914 Address after: 101, 1st Floor, Building 2, Zone 1, Courtyard 7, Liangshuihe 1st Street, Daxing District, Beijing, 100176 Patentee after: BEIJING GENOMEPRECISION TECHNOLOGY CO.,LTD. Address before: 100176 Room 401, 4th floor, building 11, 156 Jinghai 4th Road, Beijing Economic and Technological Development Zone, Daxing District, Beijing Patentee before: BEIJING SEARCH BIOTECH Co.,Ltd. |