CN102429665B - Direct blood plasma separation supporting blood collecting method - Google Patents
Direct blood plasma separation supporting blood collecting method Download PDFInfo
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- CN102429665B CN102429665B CN201110225736.5A CN201110225736A CN102429665B CN 102429665 B CN102429665 B CN 102429665B CN 201110225736 A CN201110225736 A CN 201110225736A CN 102429665 B CN102429665 B CN 102429665B
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Abstract
The invention relates to the technical field of a blood collection device, in particular to a direct blood plasma separation supporting blood collecting assembly. The assembly is composed of a support body (1), a blood absorption block (2), and a first handle (3). A blood plasma separation assembly is composed of a blood plasma storage tube (4), a hollow elastic piston (5), a filter element (6), a second handle (3), and a sealing piece (8). A blood sample storage vessel assembly is composed of a tube cover (9), a storage tube (10), and an anticoagulation (11). Compared with a natural precipitation method, the method has the advantages that: blood plasma can be obtained quickly, and time is saved; and compared with a centrifugal separating method, the method has the advantages that: special equipment is not required, and the method is very simple and convenient.
Description
[technical field]
The present invention relates to blood collection device technical field, particularly relate to a kind of directly blood collection assemblies of separated plasma.
[background technology]
Medical institutions generally obtain by gathering human vein, tremulous pulse or tip (finger finger tip, heel or ear-lobe) blood for the blood preparation of Clinical Laboratory, and the type of blood preparation is divided into whole blood, serum and plasma.Plasma specimen refer to blood in vitro after through anticoagulant process natural sedimentation or centrifugal after not containing the weak yellow liquid of blood formed element.The purposes of plasma specimen in Clinical Laboratory is very extensive, can be used for measuring biochemical composition, amynologic index, coagulation function and gene analysis of blood of human body etc.
Blood formed element accounts for 40～45% of blood total measurement (volume), and remainder is liquid component.Mostly the method for traditional separated plasma is to utilize the proportion between blood self visible component and blood plasma different, gathers after blood by natural sedimentation or the centrifugal blood plasma that obtains.
[summary of the invention]
Object of the present invention will solve the deficiencies in the prior art exactly, and a kind of blood collection method of directly separated plasma is provided.
Design for achieving the above object a kind of blood collection method of directly separated plasma, comprise blood sampling assembly, separating plasma assembly and blood sample preservation container assemblies, it is characterized in that the method is to preset the amount of sucking blood of blood sampling assembly and add blood sample to preserve the capacity of the anti-freezing liquid in container assemblies, calculate the multiple that blood is diluted, utilize blood formed element different from the physical aspect of blood plasma, the method separated plasma filtering by orientation, thus realize quantitative collection, the quantitative object of dilution, detection by quantitative.
Described method step is as follows: the sodium citrate solution that compound concentration is 32g/L, drawing 0.45mL adds blood sample to preserve in the preservation pipe (10) of container assemblies, make blood sampling assembly, wherein the amount of sucking blood of draw blood piece (2) is set as to 50 μ L, with blood specimen collection assembly draw blood 50 μ L, being placed in above-mentioned blood sample preserves in the preservation pipe (10) of container assemblies and presses first handle (3), the blood of taking can be pushed into blood sample preserves in container preservation pipe (10), mix gently, the blood plasma of separating plasma assembly is deposited pipe (4), hollow elasticity piston (5), filter element (6) is integrative-structure, its piston end is pushed to above-mentioned blood sample down to be preserved in the preservation pipe (10) of container assemblies, promote until container bottom downwards, under piston action, the blood plasma of dilution is risen to blood plasma and is deposited the inside of pipe (4) by filter element, and can not still be left on container bottom by the blood formed element of filter element, by the second handle of separating plasma assembly (3), sealing member (8) pushes blood plasma and deposits in pipe (4), until at the bottom of pipe, pressing second handle (3) fills in blood plasma by sealing member (8) and deposits the inner core aperture of pipe (4) and block, pull-out second handle (3) discards, to manage lid (9) and cover the mouth of pipe of preserving pipe (10), obtain the sodium citrate anticoagulate plasma of 9:1 dilution.
Described method step is as follows: the Lithium acid heparin solution that compound concentration is 50IU/L, drawing 0.1mL adds blood sample to preserve in the preservation pipe (10) of container assemblies, make blood sampling assembly, wherein the amount of sucking blood of draw blood piece (2) is set as to 20 μ L, with blood specimen collection assembly draw blood 20 μ L, being placed in above-mentioned blood sample preserves in the preservation pipe (10) of container assemblies and presses first handle (3), the blood of taking can be pushed into blood sample preserves in container preservation pipe (10), mix gently, the blood plasma of separating plasma assembly is deposited pipe (4), hollow elasticity piston (5), filter element (6) is integrative-structure, its piston end is pushed to above-mentioned blood sample down to be preserved in the preservation pipe (10) of container assemblies, promote until container bottom downwards, under piston action, the blood plasma of dilution is risen to blood plasma and is deposited the inside of pipe (4) by filter element, and can not still be left on container bottom by the blood formed element of filter element, by the second handle of separating plasma assembly (3), sealing member (8) pushes blood plasma and deposits in pipe (4), until at the bottom of pipe, pressing second handle (3) fills in blood plasma by sealing member (8) and deposits the inner core aperture of pipe (4) and block, pull-out second handle (3) discards, to manage lid (9) and cover the mouth of pipe of preserving pipe (10), obtain the anticoagulant heparin blood plasma of 5:1 dilution.
Described method step is as follows: the ethylenediamine tetraacetic acid,dipotassium salt solution that compound concentration is 180g/L, drawing 20 μ L adds blood sample to preserve in the preservation pipe (10) of container assemblies, make blood sampling assembly, wherein the amount of sucking blood of draw blood piece (2) is set as to 20 μ L, with blood specimen collection assembly draw blood 20 μ L, being placed in above-mentioned blood sample preserves in the preservation pipe (10) of container assemblies and presses first handle (3), the blood of taking can be pushed into blood sample preserves in container preservation pipe (10), mix gently, the blood plasma of separating plasma assembly is deposited pipe (4), hollow elasticity piston (5), filter element (6) is integrative-structure, its piston end is pushed to above-mentioned blood sample down to be preserved in the preservation pipe (10) of container assemblies, promote until container bottom downwards, under piston action, the blood plasma of dilution is risen to blood plasma and is deposited the inside of pipe (4) by filter element, and can not still be left on container bottom by the blood formed element of filter element, by the second handle of separating plasma assembly (3), sealing member (8) pushes blood plasma and deposits in pipe (4), until at the bottom of pipe, pressing second handle (3) fills in blood plasma by sealing member (8) and deposits the inner core aperture of pipe (4) and block, pull-out second handle (3) discards, to manage lid (9) and cover the mouth of pipe of preserving pipe (10), obtain the ethylenediamine tetraacetic acid,dipotassium salt anticoagulate plasma of 1:1 dilution.
Described blood sampling assembly is made up of supporter (1), draw blood piece (2), first handle (3), described supporter (1) is hollow tubular structure, supporter (1) one end is that tubule one end is extra heavy pipe, draw blood piece (2) is installed on tubule one end of supporter (1), the external diameter of draw blood piece (2) matches with the internal diameter of supporter (1), first handle (3) is installed on extra heavy pipe one end of supporter (1), first handle (3) bottom is provided with the first push rod, and the first push rod is suspended in the top of draw blood piece (2).
Described separating plasma assembly is deposited pipe (4) by blood plasma, hollow elasticity piston (5), filter element (6), second handle (3), sealing member (8) composition, described blood plasma is deposited the clear tubular parts that pipe (4) is a hollow, upper end is peristome, lower end is connected with hollow elasticity piston (5), hollow elasticity piston (5) inside is provided with filter element (6), second handle (3) one end, top is provided with bulging part, bottom is provided with the second push rod, the second push rod bottom connection sealing element (8), sealing member (8) external diameter matches with the endoporus of hollow elasticity piston (5).
Described blood sample preserve container assemblies by managing lid (9), preserve pipe (10), anticoagulant (11) form, preserves pipe (10) top connection tube lid (9), preservation is managed in (10) and is provided with anticoagulant (11).
Described supporter (1), first handle (3) are plastic injection piece; Described draw blood piece (2) adopts resilient water absorbing material to make.
The inner permeable material that adopts of described filter element (6) is made, and filter element (6) outside is provided with one deck filter membrane; Described sealing member (8) is made up of elastomeric material.
Described anticoagulant (11) adopts heparin class anticoagulant, edetate class anticoagulant or sodium citrate, potassium oxalate, hirudin.
The present invention utilizes the visible component of blood own different from the physical aspect of blood plasma, and the visible component that the method for filtering by orientation is removed blood obtains blood plasma.Its advantage is, can obtain fast blood plasma with natural sedimentation method comparison the present invention, saves time; The present invention does not need special installation with centrifugal separation comparison, very easy.In addition, owing to can presetting the amount of sucking blood of blood sampling assembly and adding blood sample to preserve the capacity of the anti-freezing liquid in container assemblies, very simply calculate the multiple that blood is diluted, thereby realize the object of quantitative collection, quantitative dilution, detection by quantitative.
[brief description of the drawings]
Fig. 1 is blood sampling assembly of the present invention;
Fig. 2 is separating plasma assembly of the present invention;
Fig. 3 is that blood sample of the present invention is preserved container assemblies;
In figure 1, supporter 2, draw blood piece 3, first handle 4, blood plasma deposit pipe 5, hollow elasticity piston 6, filter element 7, second handle 8, sealing member 9, pipe cover 10, preserve manage 11, anticoagulant.
[detailed description of the invention]
Illustrate the best mode of implementing this patent below, method of the present invention is that those skilled in the art are apparent.
Blood sampling assembly: as shown in Figure 1, blood sampling assembly is made up of three parts.Supporter (1) is hollow tubular structure, is plastic injection piece, and one end is that tubule one end is extra heavy pipe; Draw blood piece (2) is installed on tubule one end of supporter (1), makes for slightly resilient water absorbing material, and the internal diameter of external diameter and supporter (1) is suitable; First handle (3) is installed on extra heavy pipe one end of supporter (1), is moulding, and top is handle, and bottom is push rod, is suspended in the top of draw blood piece (2).The effect of supporter (1) is supporter, and draw blood piece (2) is for draw blood, and first handle (3) can be released supporter (1) by draw blood piece (2) for handle.Draw blood piece (2) for nontoxic, without separating out, make without the water absorbing material coming off, its external diameter can be equal to the internal diameter of the thinner one end of supporter (1), by adjust its length checking amount of sucking blood number.In the time that pressing downwards, first handle (3) can very easily draw blood piece (2) be released.
Separating plasma assembly: as shown in Figure 2, separating plasma assembly is made up of five parts.Wherein blood plasma is deposited pipe (4), hollow elasticity piston (5), filter element (6) and is combined, and second handle (3), sealing member (8) are combined.Blood plasma is deposited pipe (4) and is deposited pipe for blood plasma, is the clear tubular parts of a hollow, and upper end is peristome, and lower end is connected with hollow elasticity piston (5); The elastic piston that hollow elasticity piston (5) is hollow, one end is wrapped in blood plasma deposits the lower end of pipe (4), and outside is piston dress; Filter element (6) is fixed in the hollow pipe of hollow elasticity piston (5), is filter element part, and filter element inside is a kind of permeable material, outside is one deck filter membrane, the aperture of filter membrane is less than 1 μ m, because the platelet diameter of minimum is at 2～4 μ m in blood formed element, can be filtered film and intercepts; Second handle (3) is handle, and one end bulging part is handle, and stem portion is push rod, the top of push rod is sealing member (8), for elastomeric material, the interior bore portion of external diameter and hollow elasticity piston (5) is suitable, can block easily the endoporus of hollow elasticity piston (5).In the time that the inside that second handle (3), sealing member (8) assembly are deposited to pipe (4) along blood plasma promotes downwards, sealing member (8) can come off and block the endoporus of hollow elasticity piston (5) very easily.
Blood sample is preserved container assemblies: as shown in Figure 3, blood sample is preserved container assemblies and is made up of three parts.Pipe lid (9) is pipe lid, preserves pipe (10) for preserving pipe, and anticoagulant (11) is anticoagulant.Anticoagulant can be mixed with certain density aqueous solution, and the volume of liquid depends on the multiple of needs dilution.The kind of anticoagulant comprises heparin class anticoagulant (as heparin sodium, Lithium acid heparin), edetate class anticoagulant (as ethylenediamine tetraacetic acid,dipotassium salt, ethylenediaminetetraacetic acid tripotassium, disodiumedetate) and sodium citrate, potassium oxalate, hirudin etc.The internal diameter of preserving pipe (10) is slightly less than the piston-like external diameter of hollow elasticity piston (5), and the external diameter of hollow elasticity piston (5) is with respect to the internal diameter of preserving pipe (10), and interference degree is greater than 0.1mm and is less than 0.5mm cooperation closely.
Embodiment 1
Compound concentration is the sodium citrate solution of 32g/L, draws 0.45mL and adds the blood sample shown in Fig. 3 to preserve in the preservation pipe (10) of container assemblies.Make blood sampling assembly by shown in Fig. 1, wherein the amount of sucking blood of draw blood piece (2) is set as to 50 μ L(and verifies by the size of adjusting draw blood piece (2), its external diameter can be fixed, but length can be adjusted).With blood specimen collection assembly draw blood 50 μ L, be placed in above-mentioned blood sample and preserve in the preservation pipe (10) of container assemblies and press the first handle shown in Fig. 1 (3), the blood of taking can be pushed into blood sample and preserve in container preservation pipe (10), mix gently.The blood plasma of the separating plasma assembly shown in Fig. 2 is deposited pipe (4), hollow elasticity piston (5), filter element (6) is integrative-structure, its piston end is pushed to above-mentioned blood sample down to be preserved in the preservation pipe (10) of container assemblies, promote until container bottom downwards, under piston action, the blood plasma of dilution is risen to blood plasma and is deposited the inside of pipe (4) by filter element, and can not still be left on container bottom by the blood formed element of filter element, by the second handle of separating plasma assembly (3), sealing member (8) pushes blood plasma and deposits in pipe (4), until at the bottom of pipe, pressing second handle (3) fills in blood plasma by sealing member (8) and deposits the inner core aperture of pipe (4) and block, pull-out second handle (3) discards, to manage lid (9) and cover the mouth of pipe of preserving pipe (10), obtain the sodium citrate anticoagulate plasma of 9:1 dilution.
Embodiment 2
Compound concentration is the Lithium acid heparin solution of 50IU/L, draws 0.1mL and adds the blood sample shown in Fig. 3 to preserve in the preservation pipe (10) of container assemblies.Make blood sampling assembly by shown in Fig. 1, wherein the amount of sucking blood of draw blood piece (2) is set as to 20 μ L.With blood specimen collection assembly draw blood 20 μ L, be placed in above-mentioned blood sample and preserve in the preservation pipe (10) of container assemblies and press the first handle shown in Fig. 1 (3), the blood of taking can be pushed into blood sample and preserve in container preservation pipe (10), mix gently.The blood plasma of the separating plasma assembly shown in Fig. 2 is deposited pipe (4), hollow elasticity piston (5), filter element (6) is integrative-structure, its piston end is pushed to above-mentioned blood sample down to be preserved in the preservation pipe (10) of container assemblies, promote until container bottom downwards, under piston action, the blood plasma of dilution is risen to blood plasma and is deposited the inside of pipe (4) by filter element, and can not still be left on container bottom by the blood formed element of filter element, by the second handle of separating plasma assembly (3), sealing member (8) pushes blood plasma and deposits in pipe (4), until at the bottom of pipe, pressing second handle (3) fills in blood plasma by sealing member (8) and deposits the inner core aperture of pipe (4) and block, pull-out second handle (3) discards, to manage lid (9) and cover the mouth of pipe of preserving pipe (10), obtain the anticoagulant heparin blood plasma of 5:1 dilution.
Embodiment 3
Compound concentration is the ethylenediamine tetraacetic acid,dipotassium salt solution of 180g/L, draws 20 μ L and adds the blood sample shown in Fig. 3 to preserve in the preservation pipe (10) of container assemblies.Make blood sampling assembly by shown in Fig. 1, wherein the amount of sucking blood of draw blood piece (2) is set as to 20 μ L.With blood specimen collection assembly draw blood 20 μ L, be placed in above-mentioned blood sample and preserve in the preservation pipe (10) of container assemblies and press the first handle shown in Fig. 1 (3), the blood of taking can be pushed into blood sample and preserve in container preservation pipe (10), mix gently.The blood plasma of the separating plasma assembly shown in Fig. 2 is deposited pipe (4), hollow elasticity piston (5), filter element (6) is integrative-structure, its piston end is pushed to above-mentioned blood sample down to be preserved in the preservation pipe (10) of container assemblies, promote until container bottom downwards, under piston action, the blood plasma of dilution is risen to blood plasma and is deposited the inside of pipe (4) by filter element, and can not still be left on container bottom by the blood formed element of filter element, by the second handle of separating plasma assembly (3), sealing member (8) pushes blood plasma and deposits in pipe (4), until at the bottom of pipe, pressing second handle (3) fills in blood plasma by sealing member (8) and deposits the inner core aperture of pipe (4) and block, pull-out second handle (3) discards, to manage lid (9) and cover the mouth of pipe of preserving pipe (10), obtain the ethylenediamine tetraacetic acid,dipotassium salt anticoagulate plasma of 1:1 dilution.
Claims (4)
1. a direct blood collection assemblies for separated plasma, is characterized in that preserving container assemblies by blood sampling assembly, separating plasma assembly and blood sample forms,
Described blood sampling assembly is made up of supporter (1), draw blood piece (2), first handle (3), described supporter (1) is hollow tubular structure, supporter (1) one end is that tubule one end is extra heavy pipe, draw blood piece (2) is installed on tubule one end of supporter (1), the external diameter of draw blood piece (2) matches with the internal diameter of supporter (1), first handle (3) is installed on extra heavy pipe one end of supporter (1), first handle (3) bottom is provided with the first push rod, and the first push rod is suspended in the top of draw blood piece (2), described separating plasma assembly is deposited pipe (4) by blood plasma, hollow elasticity piston (5), filter element (6), second handle (3), sealing member (8) composition, described blood plasma is deposited the clear tubular parts that pipe (4) is a hollow, upper end is peristome, lower end is connected with hollow elasticity piston (5), hollow elasticity piston (5) inside is provided with filter element (6), second handle (3) one end, top is provided with bulging part, bottom is provided with the second push rod, the second push rod bottom connection sealing element (8), sealing member (8) external diameter matches with the endoporus of hollow elasticity piston (5),
Described blood sample preserve container assemblies by managing lid (9), preserve pipe (10), anticoagulant (11) form, preserves pipe (10) top connection tube lid (9), preservation is managed in (10) and is provided with anticoagulant (11).
2. a kind of directly blood collection assemblies of separated plasma as claimed in claim 1, is characterized in that described supporter (1), first handle (3) are plastic injection piece; Described draw blood piece (2) adopts resilient water absorbing material to make.
3. a kind of directly blood collection assemblies of separated plasma as claimed in claim 1, is characterized in that described filter element (6) inside adopts permeable material to make, and filter element (6) outside is provided with one deck filter membrane; Described sealing member (8) is made up of elastomeric material.
4. a kind of directly blood collection assemblies of separated plasma as claimed in claim 1, is characterized in that described anticoagulant (11) adopts heparin class anticoagulant, edetate class anticoagulant or sodium citrate, potassium oxalate, hirudin.
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| CN201110225736.5A CN102429665B (en) | 2011-08-08 | 2011-08-08 | Direct blood plasma separation supporting blood collecting method |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102772215A (en) * | 2012-08-10 | 2012-11-14 | 上海科华检验医学产品有限公司 | Vacuum blood collecting tube capable of directly separating blood serum and method for directly separating blood serum |
| CN102764133A (en) * | 2012-08-10 | 2012-11-07 | 上海科华检验医学产品有限公司 | Vacuum blood collection tube and method thereof capable of directly separating blood plasma |
| ES2686359T3 (en) | 2013-04-15 | 2018-10-17 | Becton, Dickinson And Company | Biological fluid collection device |
| BR112015026155B1 (en) * | 2013-04-15 | 2022-01-18 | Becton, Dickinson And Company | BIOLOGICAL FLUID SAMPLING TRANSFER DEVICE AND BIOLOGICAL FLUID SEPARATION AND TESTING SYSTEM |
| CN103528876A (en) * | 2013-10-29 | 2014-01-22 | 江苏德威兰医疗器械有限公司 | Serum rapid separator |
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| JP2001050952A (en) * | 1999-08-12 | 2001-02-23 | Kunimune Kogyosho:Kk | Plasma separation collection method and tool thereof |
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| CN2420976Y (en) * | 2000-05-20 | 2001-02-28 | 朱德新 | Multifunctional hemostix |
| CN2770575Y (en) * | 2004-11-25 | 2006-04-12 | 安徽信灵检验医学科技有限公司 | Disposable venous blood-taking infusion needle with safety snapclose |
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Effective date of registration: 20160127 Address after: 201609 Shanghai City, Songjiang District Road No. 59 leaf Yexie town Patentee after: Precision medical products (Shanghai) Co., Ltd. Address before: 201609 Shanghai City, Songjiang District Road No. 100 -3 Yexie town leaves Patentee before: Shanghai Kehua Laboratory Medicine Product Co., Ltd. |