CN108315259A - A kind of disposable film device of fluid bed cell culture of filling chip carrier - Google Patents
A kind of disposable film device of fluid bed cell culture of filling chip carrier Download PDFInfo
- Publication number
- CN108315259A CN108315259A CN201810436720.0A CN201810436720A CN108315259A CN 108315259 A CN108315259 A CN 108315259A CN 201810436720 A CN201810436720 A CN 201810436720A CN 108315259 A CN108315259 A CN 108315259A
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- China
- Prior art keywords
- bag body
- chip carrier
- cell culture
- closed bag
- fluid bed
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Links
- 238000004113 cell culture Methods 0.000 title claims abstract description 44
- 239000012530 fluid Substances 0.000 title claims abstract description 19
- 239000007788 liquid Substances 0.000 claims abstract description 30
- 230000008878 coupling Effects 0.000 claims abstract description 16
- 238000010168 coupling process Methods 0.000 claims abstract description 16
- 238000005859 coupling reaction Methods 0.000 claims abstract description 16
- 230000007246 mechanism Effects 0.000 claims abstract description 15
- 239000000969 carrier Substances 0.000 claims abstract description 5
- 238000000926 separation method Methods 0.000 claims abstract description 4
- 238000005476 soldering Methods 0.000 claims description 14
- 239000000835 fiber Substances 0.000 claims description 10
- 239000000463 material Substances 0.000 claims description 8
- 239000004033 plastic Substances 0.000 claims description 7
- 238000005192 partition Methods 0.000 claims description 5
- 239000011152 fibreglass Substances 0.000 claims description 4
- 239000004743 Polypropylene Substances 0.000 claims description 3
- 229920000728 polyester Polymers 0.000 claims description 3
- -1 polypropylene Polymers 0.000 claims description 2
- 229920001155 polypropylene Polymers 0.000 claims description 2
- 230000005855 radiation Effects 0.000 claims description 2
- 239000011435 rock Substances 0.000 claims 1
- 239000007789 gas Substances 0.000 abstract description 34
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 9
- 229910052760 oxygen Inorganic materials 0.000 abstract description 9
- 239000001301 oxygen Substances 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 3
- 230000008901 benefit Effects 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 16
- 238000010586 diagram Methods 0.000 description 9
- 239000012930 cell culture fluid Substances 0.000 description 8
- 230000001464 adherent effect Effects 0.000 description 6
- 230000003321 amplification Effects 0.000 description 6
- 229920002529 medical grade silicone Polymers 0.000 description 6
- 238000003199 nucleic acid amplification method Methods 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 230000010261 cell growth Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 239000000945 filler Substances 0.000 description 3
- 238000001727 in vivo Methods 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 239000000443 aerosol Substances 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000012864 cross contamination Methods 0.000 description 2
- 239000002158 endotoxin Substances 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000003746 solid phase reaction Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 230000004888 barrier function Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000010924 continuous production Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000004880 explosion Methods 0.000 description 1
- 230000003325 follicular Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 238000012946 outsourcing Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- QQONPFPTGQHPMA-UHFFFAOYSA-N propylene Natural products CC=C QQONPFPTGQHPMA-UHFFFAOYSA-N 0.000 description 1
- 125000004805 propylene group Chemical group [H]C([H])([H])C([H])([*:1])C([H])([H])[*:2] 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 239000002344 surface layer Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000003519 ventilatory effect Effects 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/14—Bags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/28—Constructional details, e.g. recesses, hinges disposable or single use
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/16—Particles; Beads; Granular material; Encapsulation
- C12M25/20—Fluidized bed
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
Abstract
The invention discloses a kind of disposable film devices of fluid bed cell culture of filling chip carrier,Including closed bag body,It is filled with several chip carriers in the internal portion of closed bag,Both sides outside closed bag body extend banding there are one being all provided with,Closed bag body extends the fixed mechanism that banding is equipped with by both sides and is fixed in biological cell culture shaking table,It is equipped with enterprising gas port in closed upper face of bag body,Gas outlet,Sample tap and at least one inlet and go out inlet,It is equipped with lower air inlet in closed bag body bottom surface,In enterprising gas port,Gas outlet,Sample tap,Inlet,It is respectively connected with sterile quick coupling on liquid outlet and lower air inlet,It is additionally provided with separating device in each liquid outlet,Closed bag body by be arranged go out the separating device at inlet by chip carrier and be trapped in closed bag it is internal,Realize the separation of cell and chip carrier.The present invention has many advantages, such as that dissolved oxygen effect is good, the whole density of cell culture is big, amplifies convenient for the industry of attached cell.
Description
Technical field
The present invention relates to technical field of cell culture, particularly relate to a kind of fluid bed cell of filling chip carrier
Cultivate disposable film device
Background technology
The bioreactor for carrying out cell culture at present, is broadly divided into fluidized bed aerosol generator and fixed bed biological respinse
Device.Fixed-bed bioreactor is to carry out cell adhere-wall culture, filler by loading filler among a fixed container
It is made of cell culture vector, and cell culture vector is typically diameter 2-11mm sheet-like fiber carriers.Cell it is adherent
Fixed bed culture technique is carried out on chip carrier earliest by U.S.'s NBS foundings of the company, but its chip carrier is typically with silk screen packet
It is firmly placed in bioreactor tank body, cell long-term is in the environment of static gas wave refrigerator, chip carrier can not be made to suspend, longer
After time, the metabolin (harmful substance) that cell generates is gathered in chip carrier and can not be discharged, it is difficult to realize attached cell
Large-scale culture, it is also difficult to directly carry out cell sampling.
Fluidized-bed reactor is a kind of to make solid particle be in by granular solids layer using gas or liquid to suspend
Motion state, the reactor of go forward side by side promoting the circulation of qi solid phase reaction process or liquid and solid phase reaction process, the purpose of fluid bed is to make air pocket
It is broken, improve the contact of gas-solid phase, reduce back-mixing, improves reaction effect, but when cultivating cell, cell is easily by air follicular rupture
Explosion damage, ventilatory capacity are unevenly distributed, and mass transfer biography oxygen is bad, causes cell growth state bad.
In addition, most of fixed-bed bioreactors currently on the market, mostly non-sterilising prods, microorganism, endotoxin
Equal Related Components content is higher, has a certain impact to subsequent sterilizing and production technology and product；And fixed bed biological respinse
Device is mostly glass stainless steel and multiple Reusability, be easy to cause the cross contamination of different batches, needs to do and largely test
Work is demonstrate,proved, relative cost is higher, while after the completion of every batch of culture, and each accessory is split and cleaning is also and its inconvenient, needs big
The working time of amount.
Existing fluidized bed aerosol generator and fixed-bed bioreactor are not easy to the industry amplification of attached cell, and
And the culture density of suspension cell is relatively low.For those skilled in the art, the culture for improving suspension cell is close
The usual of degree is to use such as Patent No. 201521086949.4《A kind of cell culture bags》This cell culture apparatus, but
Want to be combined cell culture bags with chip carrier there is presently no technical staff and be cultivated for attached cell, it is adherent by improving
The production density of cell improves the ultimate output of cell, and then it is adherent thin to overcome traditional fixed-bed bioreactor to be difficult to realize
The technical barrier of the large-scale culture of born of the same parents.
Invention content
The purpose of the present invention is to provide a kind of disposable film devices of fluid bed cell culture of filling chip carrier, you can
Metabolite is removed in time, improves the cell culture environment on chip carrier, promotes cell growth, attached cell is allowed to be suspended
Similar cultivation mechanism is cultivated, while can also keep the excellent special efficacy of correlation of attached cell, convenient for the industry amplification of attached cell.
To achieve the above object, the technical solution that the present invention takes is：A kind of fluid bed cell training of filling chip carrier
Disposable film device is supported, including closed bag body, is filled with several chip carriers, in the envelope in the closed internal portion of bag
Both sides outside enclosed bag body are all provided with there are one banding is extended, and are all provided on each extension banding there are one fixed mechanism,
The closed bag body extends the fixed mechanism that banding is equipped with by both sides and is fixed in biological cell culture shaking table, described
Closed upper face of bag body is equipped with enterprising gas port, gas outlet, sample tap and at least one inlet and goes out inlet, in the closing
Formula bag body bottom surface is equipped with lower air inlet, on the enterprising gas port, gas outlet, sample tap, inlet, liquid outlet and lower air inlet
It is respectively connected with sterile quick coupling, separating device is additionally provided in each liquid outlet, the closed bag body passes through setting
Closed bag is internal going out the separating device at inlet by chip carrier and be trapped in, and realizes the separation of cell and chip carrier.
In above-mentioned technical proposal, the closed bag body is made of upper bag sheet and lower bag sheet.
Further, it is additionally provided with one or more sensors connector in the closed bag body bottom surface；Preferably, in the envelope
Enclosed bag body bottom surface is set there are two sensor connector, one of them is the sensor connector for accessing pH value sensor probe,
Another is the sensor connector for accessing dissolved oxygen sensor probe.
Further, the touch sensor connector include soldering opening, buckle and valve block partition, the soldering opening with it is described
Closed bag body surface soldered, the buckle are connected together with the soldering opening, and the valve block partition setting is in soldering opening and envelope
At the contact site of enclosed bag body, for the buckle for sensor probe to be fixed in soldering opening, sensor probe passes through valve
Block separates culture solution directly internal with closed bag and contacts.
Further, the fixed mechanism is the fiberglass pole being encapsulated in the extension banding or is opened in the extension
Mounting hole on banding.
Further, the separating device is medical grade plastic separate mesh.
Further, the biological cell culture shaking table by shaking table control cabinet, pallet and can fluctuate or can control and shake
Dynamic shaking table seat is constituted, and the shaking table seat is fixed at the top of the shaking table control cabinet, and the pallet is arranged in the shaking table seat
Top is respectively fixed with a locking device in the both sides of the pallet inner bottom wall.
Further, the closed bag body, extension banding and sterile quick coupling are all made of medical grade plastic material and are made.
Further, each chip carrier includes that several are connected in camber and by polyester fiber and/or gathers
Sheet-like fiber blade made of propylene material, several described are opened in the sheet-like fiber blade of camber connection in external radiation
Setting.
Further, the loading amount of the chip carrier is 1g/L-100g/L.
Compared with prior art, it is an advantage of the invention that：(1) by filling chip carrier in vivo in closed bag, make thin
Born of the same parents are adherent on chip carrier, by inputting cell culture fluid in vivo to closed bag, chip carrier made to be carried in sheet with adherent
Cell on body suspends together, while having taken into account two kinds of characteristics of cell adhere-wall culture and the culture that suspends, you can obtains higher
Cell culture density, while also allowing for the amplification culture of attached cell；(2) bottom ventilation and surface layer ventilation is used to be combined logical
Gas mode increases the driving path of oxygen in a liquid, increases dissolved oxygen amount；(3) chip carrier can play similar baffle
Effect, can will be passed through generated bubble cutting in gas to liquid and fall, it is directly broken in a liquid to effectively prevent bubble
It splits, wound the risk of cell, reduce the damage to cell；(4) by filling chip carrier filler piece in vivo in closed bag
After shape carrier, increase the biography oxygen transfer path of cell culture fluid so that nutritional ingredient in liquid can it is more preferable, more fully by
Cell institute suction utilizes；(5) disposable, it without assembly, is not necessarily to dismounting, without cleaning, reduces the risk of cross contamination
And the expense of verification link, shorten production or R＆D cycle；(6) metabolite can be removed in time, improved thin on chip carrier
Born of the same parents' culture environment promotes the growth of cell；(7) training method for being added shaking table using closed bag body, is substantially reduced to cell
Caused by shearing force damage；(8) it is all made of medical grade material using closed bag body itself and its connecting component and is made, sterile,
Without microorganism, the Related Components content such as endotoxin is relatively low so that the cell holding time is relatively long, greatly reduces to rear
The influence of continuous production technology.
Description of the drawings
Fig. 1 is the top schematic diagram of the first embodiment bag body of the present invention；
Fig. 2 is the schematic bottom view of second of embodiment bag body of the present invention；
Fig. 3 is the stereoscopic schematic diagram of the first embodiment bag body of the present invention；
Fig. 4 is the stereoscopic schematic diagram of the first the embodiment bag body and shaking table of the present invention；
Fig. 5 is the top schematic diagram of second of embodiment of the present invention；
Fig. 6 is the schematic bottom view of second of embodiment of the present invention；
Fig. 7 is the stereoscopic schematic diagram of second of embodiment bag body of the present invention；
Fig. 8 is the stereoscopic schematic diagram of second the embodiment bag body and shaking table of the present invention；
Fig. 9 is first embodiment of the present invention use state lower bag body top surface connecting line schematic diagram；
Figure 10 is first embodiment of the present invention use state lower bag body bottom surface connecting line schematic diagram；
Figure 11 is the sensor connector diagrammatic cross-section of the preferred embodiment of the present invention；
Figure 12 is the structural schematic diagram of the chip carrier in the present invention；
In figure：1, closed bag body；2, chip carrier；3, extend banding；4, fiberglass pole；5, mounting hole；6, enterprising
Gas port；7, gas outlet；8, sample tap；9, inlet；10, go out inlet；11, lower air inlet；12, sterile quick coupling；13, divide
Every device；14, sensor connector；141, the sensor connector for accessing pH value sensor probe；142, for accessing dissolved oxygen
The sensor connector of sensor probe；1401, soldering opening；1402, it buckles；1403, valve block separates；15, shaking table；151, shaking table control
Case processed；152, shaking table seat；153, pallet；154, locking device；16, cell culture fluid；17, band；18, medical silicone tube；19、
Y shape presss from both sides；20, air filter；21, female Luer sampling head；22, medical heat-shrink tube；23, blind plugging.
Specific implementation mode
To make the technical means, the creative features, the aims and the efficiencies achieved by the present invention be easy to understand, with reference to
The drawings and specific embodiments, how the present invention is further explained implements.
As shown in Figures 1 to 4, a kind of fluid bed cell culture of filling chip carrier provided in an embodiment of the present invention is primary
Property film device several chip carriers 2 are filled with inside closed bag body 1 including closed bag body 1, outside closed bag body 1
The both sides in portion are all provided with there are one banding 3 is extended, and are all provided on each extension banding 3 there are one fixed mechanism, closed bag body 1 is logical
It crosses the fixed mechanism that both sides extension banding 3 is equipped with to be fixed in biological cell culture shaking table 15, be set in closed 1 top surface of bag body
There are one the gas outlet 7, one of enterprising gas port 6, one 8, inlets 9 of sample tap and one to go out inlet 10, closed
Bag body 1 bottom surface is set there are one lower air inlet 11, enterprising gas port 6, gas outlet 7, sample tap 8, inlet 9, liquid outlet 10 and under
It is respectively connected with sterile quick coupling 12 on air inlet 11, separating device 13 is additionally provided in liquid outlet 10；Closed bag body 1 passes through
Chip carrier 2 is trapped in closed bag body 1 by the separating device 13 being arranged in liquid outlet 10, realize postdigestive cell with
The separation of chip carrier 2 when being exported by liquid outlet 10, passes through liquid outlet 10 after cell falls off from chip carrier 2 when digesting
Chip carrier 2 is trapped in closed bag body 1 by interior separating device 13, and by cell as cell culture fluid 16 is together by going out liquid
10 discharge of mouth is transported in the biological cell culture tank of bigger, realizes the industrial amplification production of attached cell.
Wherein, enterprising gas port 6 and lower air inlet 11 are to add cell to bag body 1 to meet in cell cultivation process
Required gas (such as oxygen), gas outlet 7 are in order to meet in cell cultivation process, by what is discharged by cell in bag body 1
Gas (such as carbon dioxide) is discharged, and inlet 9 is added to bag body 1 and sought needed for cell to meet in cell cultivation process
Nutrient solution or other liquid, liquid outlet 10 be in order to by postdigestive attached cell as cell culture fluid 16 exits into bigger
Biological reactor in, realize the industrial amplification production of attached cell.
Wherein, sterile quick coupling 12 is the conventional sterile quick coupling of medical grade, can be obtained by outsourcing, sterile quick
Connector 12 is soldered directly on closed bag body 1.
Wherein, separating device 13 is medical grade plastic separate mesh, and the mesh size of separate mesh is that permissible cell passes through, no
Chip carrier 2 is allowed to pass through.
Wherein, closed bag body 1 is formed by welding by upper and lower two bag webs.
In order to monitor the index of correlation of the internal cell culture of closed bag, can according to practical cell culture environment demand,
It can be equipped with one or more sensors connector 14 in closed 1 bottom surface of bag body, sensor connector 14 can not also be set, such as
When disposable film device provided by the invention is entirely placed on constant carbon dioxide incubator progress small-scale cell culture,
It can not have to set sensor connector 14.
When needing in closed 1 bottom surface of bag body setting sensor connector 14, it is preferable that as illustrated in figs. 2 or 6, if
There are two sensor connector, one of them is the sensor connector 141 for accessing pH value sensor probe, another be for
The sensor connector 142 of oxygen sensor probe is held in access.
In the present invention, sensor connector 14 is touch sensor connector, as shown in Figure 10, touch sensor connector
Including soldering opening 1401, buckle 1402 and valve block partition 1403, soldering opening 1401 directly with closed 1 surface soldered of bag body, phase
The sensor probe fixation answered is threaded through in buckle 1402, and one end extend into soldering opening 1401 and separates 1403 across valve block
It is in direct contact with the cell culture fluid in closed bag body 1, the other end is connected together by buckle 1402 with soldering opening 1401.
As shown in Figures 1 to 4, fixed mechanism can be to be encapsulated in the fiberglass pole 4 extended in banding 3, such as Fig. 5 to figure
Shown in 7, fixed mechanism may be the mounting hole 5 for being opened in and extending on banding 3.
Wherein, as shown in Fig. 4 or Fig. 8, biological cell culture shaking table 15 by shaking table control cabinet 151, pallet 153 and can on
Lower shake or the shaking table seat 152 that can be rocked from side to side are constituted, and shaking table seat 152 is fixed on 151 top of shaking table control cabinet, and pallet 153 is set
It sets at the top of shaking table seat 152, a locking device 154 is respectively fixed in the both sides of 151 inner bottom wall of pallet, such as Fig. 4 and Fig. 8
Shown, which according to actual needs, can be designed to locking folder or lock-screw or other any have locking
The structure of fixed function；When cell culture, the locking device 154 that is equipped with by the both sides of 153 inner bottom wall of pallet is by closed bag
Body 1 is fixed on biological cell culture shaking table 15, and then controlling shaking table seat 152 by shaking table control cabinet 151 fluctuates or left
Right shake drives pallet 153 to be shaken together with the closed bag body 1 being fixed on pallet 153, allows cell culture fluid 13 and sheet
Carrier 2 suspends flowing together in closed bag body 1, and cell culture fluid 13 is made fully to be connect with the cell being attached on chip carrier 2
It touches and reduces the metabolin (harmful substance) that cell generates on chip carrier 2 to assemble, and then improve thin on chip carrier 2
Born of the same parents' culture environment promotes the growth of cell, allows attached cell to obtain the similar cultivation mechanism of culture that suspends, while also keeping adherent
The excellent special efficacy of correlation of cell, convenient for the industry amplification of attached cell.
Wherein, closed bag body 1, extension banding 3 and sterile quick coupling 12 are all made of medical grade plastic material and are made, example
Such as PE, PP nontoxic type plastic material.
Wherein, as shown in figure 12, each chip carrier 2 includes that several are connected in camber and by polyester fiber
And/or sheet-like fiber blade 2a made of polypropylene material, several are in outside spoke in the sheet-like fiber blade 2a of camber connection
Penetrate opening setting.
Wherein, loading amount of the chip carrier 2 in closed bag body 1 can be arranged according to the capacity requirement of closed bag body 1, and
Its loading amount range is specially 1g/L-100g/L.
In practical applications, according to actual design needs, closed 1 top and bottom of bag body can be arranged it is multiple into
Liquid mouth 9 and multiple liquid outlets 10, as shown in Figure 5.
The disposable film device of fluid bed cell culture of filling chip carrier provided by the invention, in practical applications, first
The fixed mechanism that banding 3 is equipped with can be extended by both sides to be fixed on biological cell culture shaking table 15, then by closed bag
Enterprising gas port 6, gas outlet 7, sample tap 8, inlet 9, liquid outlet 10 and the lower air inlet 11 of body 1 pass through sterile quick coupling
12, it connect, will insert in sensor connector 14 with the device for providing outside liquid, gas or withdrawal liquid, gas by the road respectively
Corresponding sensor probe is connected, and during cultivating cell, outside liquid is added as needed at any time, replaces culture solution
And provide gas needed for cell culture appropriate.
Specifically, enterprising gas port 6 can be as shown in figure 9, through 12 rapid abutting joint external feed stream pipeline of sterile quick coupling, this is outer
Portion's air inlet pipeline can include medical silicone tube 18, air filter 20 successively, then connect external gas supply device (in figure not
It shows)；
Gas outlet 7 can be as shown in figure 9, through 12 rapid abutting joint external exhaust gas pipeline of sterile quick coupling, the outer exhaust duct
Road can include medical silicone tube 18, air filter 20 successively, then connect ambient atmosphere or gas collecting apparatus (not shown)；
Inlet 9 can be as shown in figure 9, through inlet pipe outside sterile quick coupling 12 rapid abutting joint, the outside inlet tube
Road can include medical silicone tube 18, female Luer sampling head 21 successively, then connect outside liquid feeding mechanism and (do not show in figure
Go out)；
Liquid outlet 10 can be as shown in figure 9, through outlet tube road, the outside inlet tube outside sterile quick coupling 12 rapid abutting joint
Road can include medical heat-shrink tube 22, blind plugging 23 successively, then connect external liquid collector (not shown)；
Lower air inlet 11 can be as shown in Figure 10, through 12 rapid abutting joint external feed stream pipeline of sterile quick coupling, outside this into
Air pipe can include medical silicone tube 18, air filter 20 successively, then connect external gas supply device and (do not show in figure
Go out).
As shown in Figure 9 and Figure 10, it is provided with 19 control of Y shape folder on the medical silicone tube 18 on each pipeline and medical heat-shrink tube 22
The disengaging of liquids and gases processed is tightly connected by band 17 between pipeline components.
Finally illustrate, the above is only the implementation of the present invention example, is not intended to limit the scope of the invention, it is every
Using equivalent structure or equivalent flow shift made by description of the invention and accompanying drawing content, it is applied directly or indirectly in other
Relevant technical field, is included within the scope of the present invention.
Claims (10)
1. a kind of disposable film device of fluid bed cell culture of filling chip carrier, including closed bag body (1), feature exists
In：Several chip carriers (2) are filled with inside the closed bag body (1), in the external both sides of the closed bag body (1)
Be all provided with there are one extend banding (3), be all provided on each extensions banding (3) there are one fixed mechanism, the closed bag
Body (1) extends the fixed mechanism that banding (3) is equipped with by both sides and is fixed in biological cell culture shaking table (15), in the envelope
Enclosed bag body (1) top surface is equipped with enterprising gas port (6), gas outlet (7), sample tap (8) and at least one inlet (9) and goes out feed liquor
Mouth (10) is equipped with lower air inlet (11) in closed bag body (1) bottom surface, in the enterprising gas port (6), gas outlet (7), takes
It is respectively connected with sterile quick coupling (12) on sample mouth (8), inlet (9), liquid outlet (10) and lower air inlet (11), in each institute
It states and is additionally provided with separating device (13) in liquid outlet (10), the closed bag body (1) is divided by what is be arranged in liquid outlet (10)
Chip carrier (2) is trapped in closed bag body (1) every device (13), realizes the separation of cell and chip carrier (2).
2. the disposable film device of fluid bed cell culture of filling chip carrier according to claim 1, it is characterised in that：
The closed bag body (1) is made of upper bag sheet and lower bag sheet.
3. the disposable film device of fluid bed cell culture of filling chip carrier according to claim 1, it is characterised in that：
It is additionally provided with one or more sensors connector (14) in closed bag body (1) bottom surface.
4. the disposable film device of fluid bed cell culture of filling chip carrier according to claim 1, it is characterised in that：
The sensor connector (14) is touch sensor connector, and the touch sensor connector includes soldering opening (1401), card
Detain (1402) and valve block partition (1403), the soldering opening (1401) and closed bag body (1) surface soldered, the buckle
(1402) be connected together with the soldering opening (1401), valve block partition (1403) setting soldering opening (1401) with it is closed
At the contact site of bag body (1).
5. the disposable film device of fluid bed cell culture of filling chip carrier according to claim 1, it is characterised in that：
The fixed mechanism (4) is the fiberglass pole (4) being encapsulated in the extension banding (3) or is opened in the extension banding
(3) mounting hole (5) on.
6. the disposable film device of fluid bed cell culture of filling chip carrier according to claim 1, it is characterised in that：
The separating device (13) is medical grade plastic separate mesh.
7. the disposable film device of fluid bed cell culture of filling chip carrier according to claim 1, it is characterised in that：
The biological cell culture shaking table (15) is by shaking table control cabinet (151), pallet (153) and can fluctuate or can rock from side to side
Shaking table seat (152) constitute, the shaking table seat (152) is fixed at the top of the shaking table control cabinet (151), the pallet (153)
It is arranged at the top of the shaking table seat (152), a locking device is respectively fixed in the both sides of the pallet (151) inner bottom wall
(154)。
8. the disposable film device of fluid bed cell culture of filling chip carrier according to claim 1, it is characterised in that：
The closed bag body (1), extension banding (3) and sterile quick coupling (12) are all made of medical grade plastic material and are made.
9. the disposable film device of fluid bed cell culture of filling chip carrier according to claim 1, it is characterised in that：
Each chip carrier (2) includes that several are connected in camber and is made of polyester fiber and/or polypropylene material
Sheet-like fiber blade (2a), described several are in be set to external radiation opening in sheet-like fiber blades (2a) of camber connection
It sets.
10. the disposable film device of fluid bed cell culture of filling chip carrier according to claim 1, feature exist
In：The loading amount of the chip carrier (2) is 1g/L-100g/L.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810436720.0A CN108315259B (en) | 2018-05-09 | 2018-05-09 | Fluidized bed cell culture disposable membrane device filled with sheet-shaped carrier |
| PCT/CN2019/084600 WO2019214465A1 (en) | 2018-05-09 | 2019-04-26 | Fluidized bed cell culture disposable membrane device filled with flaky carriers |
| JP2020600192U JP3233421U (en) | 2018-05-09 | 2019-04-26 | Fluidized bed cell culture disposable membrane device for filling sheet-like carriers |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810436720.0A CN108315259B (en) | 2018-05-09 | 2018-05-09 | Fluidized bed cell culture disposable membrane device filled with sheet-shaped carrier |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108315259A true CN108315259A (en) | 2018-07-24 |
| CN108315259B CN108315259B (en) | 2024-01-23 |
Family
ID=62895992
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810436720.0A Active CN108315259B (en) | 2018-05-09 | 2018-05-09 | Fluidized bed cell culture disposable membrane device filled with sheet-shaped carrier |
Country Status (3)
| Country | Link |
|---|---|
| JP (1) | JP3233421U (en) |
| CN (1) | CN108315259B (en) |
| WO (1) | WO2019214465A1 (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108998344A (en) * | 2018-08-06 | 2018-12-14 | 武汉赛科成科技有限公司 | A kind of method of continuous large-scale production recombined adhenovirus |
| WO2019214465A1 (en) * | 2018-05-09 | 2019-11-14 | 武汉赛科成科技有限公司 | Fluidized bed cell culture disposable membrane device filled with flaky carriers |
| CN112899165A (en) * | 2019-11-19 | 2021-06-04 | 上海三维生物技术有限公司 | Cell inoculation mode of high-density adherent cell culture system |
| WO2021114085A1 (en) * | 2019-12-10 | 2021-06-17 | 苏州生动细胞生物科技有限公司 | Fixable cell culture assembly |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021114087A1 (en) * | 2019-12-10 | 2021-06-17 | 苏州生动细胞生物科技有限公司 | Small-volume cell culture bag |
| CN111500453A (en) * | 2020-04-30 | 2020-08-07 | 龙岩学院 | Sealed cultivation device for biological cell cultivation and use method thereof |
| CN114317397A (en) * | 2021-12-02 | 2022-04-12 | 罗火生 | Paper sheet carrier fixed bed cultured cell passage tank transfer tank amplification method |
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Also Published As
| Publication number | Publication date |
|---|---|
| WO2019214465A1 (en) | 2019-11-14 |
| CN108315259B (en) | 2024-01-23 |
| JP3233421U (en) | 2021-08-12 |
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