JP3233421U - Fluidized bed cell culture disposable membrane device for filling sheet-like carriers - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a fluid bed cell culture disposable membrane device for filling a sheet-like carrier having an excellent oxygen-dissolving effect, a large final cell culture density, and facilitating the industrialization of adherent cells. SOLUTION: A sealed bag body 1 is included, a small amount of sheet-like carrier is filled inside the sealed bag body, and one extending edge seal 3 is installed on both sides of the outside of the sealed bag body. The closed bag is fixed to the biological cell culture shaker by a fixing mechanism installed on the extending edge seals on both sides, and the upper intake port 6, the exhaust port 7, the sampling port 8 and at least 1 are attached to the top surface of the closed bag body. Two liquid inlets 9 and liquid outlets 10 are installed, a lower intake port is installed on the bottom surface of the sealed bag body, and the upper intake port, the exhaust port, the sampling port, the liquid inlet, the liquid outlet and the lower intake port are all sterile. A quick connector is connected, a separation device 13 is further installed in each liquid outlet, and the sheet-like carrier is stopped in the closed-type bag by the separation device in which the sealed bag body is installed in the liquid outlet, and the cells and the sheet-like carrier are stopped. Achieve the separation of. [Selection diagram] Fig. 1

Description

The present invention relates to cell culture, specifically, a fluidized bed cell culture disposable membrane device for filling a sheet-like carrier.

Conventional biological reactors for cell culture are mainly divided into fluidized bed biological reactors and fixed bed biological reactors. A fixed-bed biological reactor is used to perform cell-adhesive culture by filling a fixed container with a filler. The filler is composed of a cell culture carrier, and the cell culture carrier is generally in the form of a sheet having a diameter of 2 to 11 mm. It is a fiber carrier. The technique of attaching cells to a sheet-like carrier for fixed-bed culture was first created by NBS in the United States, but the sheet-like carrier is generally wrapped in a thread net and placed in a bioreactor tank. Since the cells are in a static culture environment for a long time, the sheet-like carrier cannot be suspended, and after a long period of time, the metabolites (harmful substances) produced by the cells gather in the sheet-like carrier. It becomes difficult to realize large-scale culture of adherent cells, and it becomes difficult to directly collect cell samples.

A fluidized bed reactor is a reactor in which a gas or liquid is passed through a particulate solid layer to bring solid particles into a floating motion state, and a gas solid phase reaction process or a liquid solid phase reaction process is performed. The purpose of the fluidized bed reactor is It is to crush large bubbles to improve the contact of the gas solid phase, reduce back mixing, and improve the reaction effect, but during cell culture, the cells are easily damaged by the crushing explosion of large bubbles, and aeration Since the amount distribution is non-uniform and substance transfer and oxygen transfer are not desirable, the cell growth state deteriorates.

Most of the fixed-bed biological reactors on the market are non-sterilized products, and the content of related components such as microorganisms and endotoxins is high, which has a certain effect on the subsequent sterilization and production process and products. Furthermore, fixed-bed bioreactors are mostly glass-stainless steel materials, are used repeatedly, are prone to cross-contamination between batches, require a large amount of inspection work, are relatively expensive, and each After the batch culture is complete, removing and cleaning each part is also very inconvenient and requires a lot of labor time.

Neither conventional fluidized bed bioreactors nor fixed bed bioreactors can easily realize the industrialized and expanded production of adherent cells, and the culture density of suspended cells is relatively low. For those skilled in the art, in order to increase the culture density of suspended cells, a cell culture device such as "cell culture bag" of patent number 2015210869494. To overcome the technical challenge that it is difficult to achieve large-scale culture of adherent cells by conventional fixed-bed bioreactors by increasing the final production of cells in order to improve the production density of adherent cells. Has not yet been conceived by engineers.

The object of the present invention is to immediately remove metabolites, improve the cell culture environment on the sheet-like carrier, promote cell growth, realize a culture mechanism similar to suspension culture in adherent cells, and make adherent cells. It is an object of the present invention to provide a fluid bed cell culture disposable membrane device for filling a sheet-like carrier that retains the relevant superior effects and can easily realize the industrialized and expanded production of adherent cells.

The technical solutions adopted by the present invention to achieve the above object are as follows. In a fluidized bed cell culture disposable membrane device for filling a sheet-like carrier including a closed-type bag body, a small amount of the sheet-like carrier is filled inside the closed-type bag body, and both sides outside the closed-type bag body are filled. In each case, one extending edge seal is installed, one fixing mechanism is installed in each of the extending edge seals, and the sealed bag body is installed in the extending edge seals on both sides. It is fixed to a cell culture shaker, and an upper intake port, an exhaust port, a sampling port, and at least one liquid inlet and a liquid outlet are provided on the top surface of the closed type bag body, and a lower intake port is provided on the bottom surface of the closed type bag body. Is installed, a sterile quick connector is connected to each of the upper intake port, the exhaust port, the sampling port, the liquid inlet, the liquid outlet, and the lower intake port, and a separation device is further installed in each of the liquid outlets. The sheet-like carrier is stopped in the closed-type bag by a separation device in which the sealed bag is installed in the liquid outlet to realize separation of cells and the sheet-like carrier.

In the above technical solution, the sealed bag body is composed of an upper bag body and a lower bag body.

Further, one or more sensor connectors are further installed on the bottom surface of the sealed bag body, two sensor connectors are installed on the bottom surface of the sealed bag body, and one of the sensor connectors is used as a pH value sensor probe. It is for connecting, and the other sensor connector is for connecting the oxygen-dissolved sensor probe.

Further, the sensor connector is a contact type sensor connector, the contact type sensor connector includes a welding port, an engaging tool and a valve-shaped partition, and the welding port is welded to the surface of the sealed bag body, and the engaging The tool is engaged and connected to the weld port, the valve-shaped partition is installed at the contact portion between the weld port and the closed bag body, and the engage tool is for fixing the sensor probe to the weld port, and the sensor. The probe passes through the valvular partition and comes into direct contact with the culture solution in the closed sac.

Further, the fixing mechanism is a glass fiber rod mounted in the extending edge seal or a fixing hole provided in the extending edge seal.

Further, the separation device is a medical plastic separation net.

Further, the biological cell culture shaker is composed of a shaker control box, a tray and a shaker base that can swing up and down or left and right, the shaker base is fixed to the top of the shaker control box, and the tray is said. Installed on the top of the shaker base, one locking device is fixed to each side of the inner bottom wall of the tray.

Further, the sealed bag body, the extending edge seal and the sterile quick connector are all made of medical plastic material.

Further, each of the sheet-shaped carriers includes some sheet-shaped fiber blades made of polyester fiber and / or polypropylene material in which the sheet-shaped carriers are connected in an arc shape, and the few sheet-shaped fiber blades connected in an arc shape are formed. It is installed so that it radiates outward.

Further, the filling amount of the sheet-shaped carrier is 1 g / L to 100 g / L.

Compared with the prior art, the present invention has the following advantages. (1) A sheet-like carrier is filled in a closed bag to attach cells to the sheet-like carrier, and a cell culture solution is added to the closed-type bag to suspend both the sheet-like carrier and the cells attached to the sheet-like carrier. As a result, it is possible to simultaneously combine the two characteristics of cell adhesion culture and suspension culture, obtain a higher cell culture density, and facilitate the expansion culture of adherent cells. (2) By adopting a ventilation method that combines bottom ventilation and surface ventilation, the traveling passage with oxygen liquid was increased and the amount of dissolved oxygen was increased. (3) The sheet-like carrier can perform an action similar to that of a baffle, can cut bubbles generated by introducing a gas into a liquid, and there is a risk that the bubbles will directly break and explode in the liquid and damage cells. Was effectively avoided and cell damage was reduced. (4) By filling the sealed bag with a sheet-like carrier, the oxygen transfer and substance transmission passages of the cell culture solution are increased, whereby the nutrient components in the liquid are more preferably and sufficiently absorbed and utilized by the cells. Will be possible. (5) It became disposable, did not need to be assembled, removed, or cleaned, the risk of cross-contamination and the cost of the inspection process were reduced, and the production or R & D cycle was shortened. (6) Metabolites can be immediately removed, the cell culture environment on the sheet-like carrier can be improved, and cell growth can be promoted. (7) A culture method combining a closed bag and a shaker was adopted to significantly reduce shear damage to cells. (8) The closed bag body and its connecting members are both made of medical materials, are free of bacteria and microorganisms, and have a relatively low content of related components such as endotoxin, so that the cell storage time is relative. For a long time, the impact on the subsequent production process was greatly reduced.

It is a top view of the bag body of the 1st Example of this invention. It is a bottom view of the bag body of the 2nd Example of this invention. It is a three-dimensional schematic diagram of the bag body of the 1st Example of this invention. It is a three-dimensional schematic diagram of the bag body and the shaker of the first embodiment of this invention. It is a top view of the 2nd Example of this invention. It is a bottom view of the 2nd Example of this invention. It is a three-dimensional schematic diagram of the bag body of the 2nd Example of this invention. It is a three-dimensional schematic diagram of the bag body and the shaker of the second embodiment of the present invention. It is a schematic diagram of the connecting pipeline of the top surface of the bag body in the use state of the 1st Example of this invention. It is a schematic diagram of the connecting conduit of the bottom surface of the bag body in the use state of the 1st Example of this invention. It is sectional drawing of the sensor connector of a preferable embodiment of this invention. It is a structural schematic diagram of the sheet-like carrier in this invention.

In order to make it easier to understand the technical means, creative features, achievement objectives, and functional effects realized by the present invention, the following will further explain how the present invention is implemented with reference to the drawings and specific embodiments. It will be described in detail.

As shown in FIGS. 1 to 4, the liquid bed cell culture disposable membrane device for filling the sheet-like carrier provided in the embodiment of the present invention includes the closed type bag body 1 and includes the closed type bag body 1. A small amount of sheet-like carrier 2 is filled inside, and one extending edge seal 3 is installed on both sides of the outside of the sealed bag body 1, and each extending edge seal 3 has one fixing mechanism. The sealed bag 1 is fixed to the biological cell culture shaker 15 by a fixing mechanism installed on the extending edge seals 3 on both sides, and one upper intake port 6 and one on the top surface of the closed bag 1. Exhaust port 7, one sampling port 8, one liquid inlet 9 and one liquid outlet 10 are installed, one lower intake port 11 is installed on the bottom surface of the sealed bag body 1, upper intake port 6, exhaust. A sterile quick connector 12 is connected to each of the port 7, the sampling port 8, the liquid inlet 9, the liquid outlet 10, and the lower intake port 11, and a separation device 13 is further installed in the liquid outlet 10, and the sealed bag body 1 is formed. The sheet-like carrier 2 is stopped in the sealed bag 1 by the separation device 13 installed in the liquid outlet 10 to realize the separation of the cells after dissociation and the sheet-like carrier 2, that is, the cells are in the sheet-like carrier at the time of dissociation. When separated from 2 and then discharged via the liquid outlet 10, the sheet-like carrier 2 is stopped in the closed bag 1 by the separating device 13 in the liquid outlet 10, but the cells are accompanied by the cell culture solution 16. It is discharged through the liquid outlet 10 and transported to a larger biological cell culture tank to realize the industrialized and expanded production of adherent cells.

Here, the upper intake port 6 and the lower intake port 11 are for adding a gas (for example, oxygen) necessary for cells in the cell culture process to the bag body 1, and the exhaust port 7 is a bag body in the cell culture process. The purpose is for discharging the gas (for example, carbon dioxide) discharged from the cells in 1 and the liquid inlet 9 is for adding a nutrient solution or other liquid necessary for the cells in the cell culture process to the bag body 1. The liquid outlet 10 is for discharging the adherent cells after dissociation together with the cell culture solution 16 and transporting them to a larger biological reaction tank to realize the industrialized and expanded production of the adherent cells.

Here, the sterile quick connector 12 is a conventional medical sterile quick connector, which is commercially available, and the sterile quick connector 12 is directly welded to the sealed bag body 1.

Here, the separation device 13 is a medical plastic separation network, and the network of the separation network is so large that cells can pass through it, but the sheet-like carrier 2 cannot pass through.

Here, the sealed bag body 1 is formed by welding the upper bag body and the lower bag body.

One or more sensor connectors 14 may be installed on the bottom surface of the closed cell 1 according to the actual cell culture environment requirements in order to monitor the relevant indicators of cell culture in the closed cell. , The sensor connector 14 does not need to be installed. For example, when the entire disposable membrane device provided by the present invention is placed in a constant carbon dioxide culture box for small-scale cell culture, the sensor connector 14 does not need to be installed. May be good.

When it is necessary to install the sensor connector 14 on the bottom surface of the sealed bag body 1, preferably, as shown in FIG. 2 or FIG. 6, two sensor connectors are installed, and one of the sensor connectors 141 has a pH value. It is for connecting the sensor probe, and the other sensor connector 142 is for connecting the oxygen-dissolved sensor probe.

In the present invention, the sensor connector 14 is a contact type sensor connector, and as shown in FIG. 10, the contact type sensor connector includes a welding port 1401, an engaging tool 1402, and a valve-shaped partition 1403, and the welding port 1401 is directly connected. The sealed bag is welded to the surface of the sealed bag 1, the corresponding sensor probe is inserted into the engaging tool 1402 and fixed, and one end extends into the weld port 1401 and passes through the valve-shaped partition 1403. It comes into direct contact with the cell culture solution in 1, and the other end is engaged and connected to the welding port 1401 by the engaging tool 1402.

As shown in FIGS. 1 to 4, the fixing mechanism may be a glass fiber rod 4 mounted in the extending edge seal 3, and as shown in FIGS. 5 to 7, the fixing mechanism is the extending edge seal 3. It may be the fixing hole 5 provided in.

Here, as shown in FIG. 4 or 8, the biological cell culture shaker 15 is composed of a shaker control box 151, a tray 153, and a shaker base 152 that can swing up and down or swing left and right, and the shaker base 152 includes a shaker base 152. Fixed to the top of the shaker control box 151, the tray 153 was installed on the top of the shaker base 152, and one locking device 154 was fixed to each side of the inner bottom wall of the tray 151, as shown in FIGS. 4 and 8. , This locking device 154 may be designed as a structure with a lock clip or lock screw or any other locking feature, depending on the actual requirements, and was installed on both sides of the inner bottom wall of the tray 153 during cell culture. The sealed bag 1 is fixed to the biological cell culture shaker 15 by the lock device 154, and then the shaker base 152 is controlled to swing up and down or left and right by the shaker control box 151, and the tray 153 and the tray 153 are controlled. The closed-type bag body 1 fixed to the cell culture solution 13 and the sheet-shaped carrier 2 are allowed to float and flow together in the closed-type bag body 1 so that the cell culture solution 13 and the sheet-like carrier 2 are floated together. The cells adhering to the cells are sufficiently brought into contact with each other to reduce the concentration of the metabolites (harmful substances) produced by the cells on the sheet-like carrier 2, thereby improving the cell culture environment on the sheet-like carrier 2 and the cells. Promotes the growth of adherent cells, realizes a culture mechanism similar to suspension culture in adherent cells, retains the favorable effects associated with adherent cells, and facilitates the industrialized and expanded production of adherent cells.

Here, the sealed bag body 1, the extending edge seal 3, and the sterile quick connector 12 are all made of a medical plastic material, for example, a non-toxic plastic material such as PE or PP.

Here, as shown in FIG. 12, each sheet-like carrier 2 includes some sheet-like fiber blades 2a made of polyester fiber and / or polypropylene material connected in an arc shape, and is connected in an arc shape. A few sheet-shaped fiber blades 2a are installed so as to radiate outward.

Here, the filling amount of the sheet-shaped carrier 2 into the closed bag body 1 can be set according to the capacity requirement of the closed bag body 1, and the filling amount range is specifically 1 g / L to 100 g /. It is L.

In actual use, as shown in FIG. 5, a plurality of liquid inlets 9 and a plurality of liquid outlets 10 may be installed on the top surface and the bottom surface of the sealed bag body 1 according to actual design requirements. good.

The fluidized bed cell culture disposable membrane device for filling the sheet-like carrier provided in the present invention is actually applied to the biological cell culture shaker 15 by the fixation mechanism previously installed on the extending edge seals 3 on both sides. It may be fixed, and then the upper intake port 6, the exhaust port 7, the sampling port 8, the liquid inlet 9, the liquid outlet 10, and the lower intake port 11 of the sealed bag body 1 are routed by a sterile quick connector 12, respectively. By connecting to a device that provides an external liquid or gas or recovers the liquid or gas, a sensor probe corresponding to the sensor connector 14 is inserted, and the external liquid is timely and needed in the process of cultured cells. Is added, the culture medium is replaced, and the appropriate gas required for cell culture is provided.

Specifically, as shown in FIG. 9, the upper intake port 6 may be quickly connected to the external air supply line by the sterile quick connector 12, and the external air supply line is sequentially connected to the medical silicone tube 18 and the air filter. 20 may be included and then connected to an external gas supply device (not shown).

As shown in FIG. 9, the exhaust port 7 may be quickly connected to the external exhaust line by the sterile quick connector 12, and the external exhaust line may include the medical grade silicone tube 18 and the air filter 20 in this order. Next, it is connected to an external air or gas collector (not shown).

As shown in FIG. 9, the liquid inlet 9 may be rapidly connected to the external liquid supply line by the sterile quick connector 12, which in turn connects the medical grade silicone tube 18 and the luer connector sampling head 21. May include and then connect to an external liquid feeder (not shown).

As shown in FIG. 9, the liquid outlet 10 may be rapidly coupled to an external liquid discharge line by a sterile quick connector 12, which in turn includes a medical heat shrink tube 22 and a blind plug 23. Then connect to an external liquid collector (not shown).

As shown in FIG. 10, the lower intake port 11 may be quickly connected to the external air supply line by the sterile quick connector 12, and the external air supply line may include the medical grade silicone tube 18 and the air filter 20 in this order. Then, it is connected to an external gas supply device (not shown).

As shown in FIGS. 9 and 10, a Y-shaped clip 19 for controlling the inflow and outflow of liquid and gas is installed on the medical silicone tube 18 and the medical heat shrinkable tube 22 in each conduit, and the members of the conduit are provided. They are hermetically connected by a binding band 17.

Finally, the above does not limit the scope of the present invention, but merely an embodiment of the present invention, and the structure or equivalent of the equivalent effect made based on the contents of the specification and drawings of the present invention. It is explained that the flow change of the effect, or the direct or indirect application to other related technical fields, are all included in the scope of protection of the present invention for the same reason.

[Additional Notes]
[Appendix 1]
In a fluidized bed cell culture disposable membrane device for filling a sheet-like carrier containing the closed-type bag (1), a small amount of the sheet-like carrier (2) is filled inside the closed-type bag (1), and the above. One extending edge seal (3) is installed on both outer sides of the sealed bag body (1), and one fixing mechanism is installed on each of the extending edge seals (3), and the sealing is performed. The formula bag (1) is fixed to the biological cell culture shaker (15) by a fixing mechanism installed on the extending edge seals (3) on both sides, and the upper intake port (1) is attached to the top surface of the sealed bag (1). 6), an exhaust port (7), a sampling port (8), and at least one liquid inlet (9) and a liquid outlet (10) are installed, and a lower intake port (11) is provided on the bottom surface of the sealed bag body (1). ) Is installed, and sterile quick connectors are provided at the upper intake port (6), exhaust port (7), sampling port (8), liquid inlet (9), liquid outlet (10), and lower intake port (11). (12) is connected, a separation device (13) is further installed in each of the liquid outlets (10), and a separation device (13) in which the sealed bag body (1) is installed in the liquid outlet (10). ) Stops the sheet-like carrier (2) in the closed bag body (1) to realize the separation of the cells and the sheet-like carrier (2).
A fluidized bed cell culture disposable membrane device for filling a sheet-like carrier.

[Appendix 2]
The sealed bag body (1) is composed of an upper bag body and a lower bag body.
A fluidized bed cell culture disposable membrane device for filling the sheet-like carrier according to Appendix 1, wherein the fluidized bed cell culture is disposable.

[Appendix 3]
One or more sensor connectors (14) are further installed on the bottom surface of the sealed bag body (1).
A fluidized bed cell culture disposable membrane device for filling the sheet-like carrier according to Appendix 1, wherein the fluidized bed cell culture is disposable.

[Appendix 4]
The sensor connector (14) is a contact type sensor connector, the contact type sensor connector includes a welding port (1401), an engaging tool (1402) and a valve-shaped partition (1403), and the welding port (1401) is the welding port (1401). It is welded to the surface of the sealed bag body (1), the engaging tool (1402) is engaged and connected to the welding port (1401), and the valve-shaped partition (1403) is connected to the welding port (1401). Installed at the contact site of the body (1),
A fluidized bed cell culture disposable membrane device for filling the sheet-like carrier according to Appendix 1, wherein the fluidized bed cell culture is disposable.

[Appendix 5]
The fixing mechanism (4) is a glass fiber rod (4) mounted in the extending edge seal (3) or a fixing hole (5) provided in the extending edge seal (3).
A fluidized bed cell culture disposable membrane device for filling the sheet-like carrier according to Appendix 1, wherein the fluidized bed cell culture is disposable.

[Appendix 6]
The separation device (13) is a medical plastic separation net.
A fluidized bed cell culture disposable membrane device for filling the sheet-like carrier according to Appendix 1, wherein the fluidized bed cell culture is disposable.

[Appendix 7]
The biological cell culture shaker (15) is composed of a shaker control box (151), a tray (153), and a shaker base (152) that can swing up and down or left and right, and the shaker base (152) is the shaker base (152). Fixed to the top of the shaker control box (151), the tray (153) is installed on the top of the shaker base (152), one locking device (154) on each side of the inner bottom wall of the tray (153). Is fixed,
A fluidized bed cell culture disposable membrane device for filling the sheet-like carrier according to Appendix 1, wherein the fluidized bed cell culture is disposable.

[Appendix 8]
The sealed bag body (1), extending edge seal (3) and sterile quick connector (12) are all made of medical plastic material.
A fluidized bed cell culture disposable membrane device for filling the sheet-like carrier according to Appendix 1, wherein the fluidized bed cell culture is disposable.

[Appendix 9]
Each of the sheet-like carriers (2) includes some sheet-like fiber blades (2a) made of polyester fiber and / or polypropylene material connected in an arc shape, and the few sheets connected in an arc shape. The fiber blades (2a) are installed so as to radiate outward.
A fluidized bed cell culture disposable membrane device for filling the sheet-like carrier according to Appendix 1, wherein the fluidized bed cell culture is disposable.

[Appendix 10]
The filling amount of the sheet-shaped carrier (2) is 1 g / L to 100 g / L.
A fluidized bed cell culture disposable membrane device for filling the sheet-like carrier according to Appendix 1, wherein the fluidized bed cell culture is disposable.

1: Sealed bag body 2: Sheet-like carrier 3: Extended edge seal 4: Glass fiber rod 5: Fixed hole, 6: Upper intake port, 7: Exhaust port, 8: Sampling port, 9: Liquid inlet, 10: Liquid outlet, 11: Lower intake port, 12: Sterile quick connector, 13: Separator, 14: Sensor connector, 141: Sensor connector for connecting pH value sensor probe, 142: Oxygen-dissolved sensor probe Sensor connector for connecting, 1401: Welding port, 1402: Engagement tool, 1403: Valve-shaped partition, 15: Shaker, 151: Shaker control box, 152: Shaker base, 153: Tray, 154: Locking device, 16 : Cell culture solution, 17: Binding band, 18: Medical silicon tube, 19: Y-shaped clip, 20: Air filter, 21: Luer connector sampling head, 22: Medical heat shrinkable tube, 23: Blind plug

Claims (10)

In a fluidized bed cell culture disposable membrane device for filling a sheet-like carrier containing the closed-type bag (1), a small amount of the sheet-like carrier (2) is filled inside the closed-type bag (1), and the above. One extending edge seal (3) is installed on both outer sides of the sealed bag body (1), and one fixing mechanism is installed on each of the extending edge seals (3), and the sealing is performed. The formula bag (1) is fixed to the biological cell culture shaker (15) by a fixing mechanism installed on the extending edge seals (3) on both sides, and the upper intake port (1) is attached to the top surface of the sealed bag (1). 6), an exhaust port (7), a sampling port (8), and at least one liquid inlet (9) and a liquid outlet (10) are installed, and a lower intake port (11) is provided on the bottom surface of the sealed bag body (1). ) Is installed, and sterile quick connectors are provided at the upper intake port (6), exhaust port (7), sampling port (8), liquid inlet (9), liquid outlet (10), and lower intake port (11). (12) is connected, a separation device (13) is further installed in each of the liquid outlets (10), and a separation device (13) in which the sealed bag body (1) is installed in the liquid outlet (10). ) Stops the sheet-like carrier (2) in the closed bag body (1) to realize the separation of the cells and the sheet-like carrier (2).
A fluidized bed cell culture disposable membrane device for filling a sheet-like carrier. The sealed bag body (1) is composed of an upper bag body and a lower bag body.
A fluidized bed cell culture disposable membrane device for filling the sheet-like carrier according to claim 1. One or more sensor connectors (14) are further installed on the bottom surface of the sealed bag body (1).
A fluidized bed cell culture disposable membrane device for filling the sheet-like carrier according to claim 1. The sensor connector (14) is a contact type sensor connector, the contact type sensor connector includes a welding port (1401), an engaging tool (1402) and a valve-shaped partition (1403), and the welding port (1401) is the welding port (1401). It is welded to the surface of the sealed bag body (1), the engaging tool (1402) is engaged and connected to the welding port (1401), and the valve-shaped partition (1403) is connected to the welding port (1401). Installed at the contact site of the body (1),
A fluidized bed cell culture disposable membrane device for filling the sheet-like carrier according to claim 1. The fixing mechanism (4) is a glass fiber rod (4) mounted in the extending edge seal (3) or a fixing hole (5) provided in the extending edge seal (3).
A fluidized bed cell culture disposable membrane device for filling the sheet-like carrier according to claim 1. The separation device (13) is a medical plastic separation net.
A fluidized bed cell culture disposable membrane device for filling the sheet-like carrier according to claim 1. The biological cell culture shaker (15) is composed of a shaker control box (151), a tray (153), and a shaker base (152) that can swing up and down or left and right, and the shaker base (152) is the shaker base (152). Fixed to the top of the shaker control box (151), the tray (153) is installed on the top of the shaker base (152), one locking device (154) on each side of the inner bottom wall of the tray (153). Is fixed,
A fluidized bed cell culture disposable membrane device for filling the sheet-like carrier according to claim 1. The sealed bag body (1), extending edge seal (3) and sterile quick connector (12) are all made of medical plastic material.
A fluidized bed cell culture disposable membrane device for filling the sheet-like carrier according to claim 1. Each of the sheet-like carriers (2) includes some sheet-like fiber blades (2a) made of polyester fiber and / or polypropylene material connected in an arc shape, and the few sheets connected in an arc shape. The fiber blades (2a) are installed so as to radiate outward.
A fluidized bed cell culture disposable membrane device for filling the sheet-like carrier according to claim 1. The filling amount of the sheet-shaped carrier (2) is 1 g / L to 100 g / L.
A fluidized bed cell culture disposable membrane device for filling the sheet-like carrier according to claim 1.

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