CN205473765U - One -way sampler - Google Patents

Abstract

The utility model relates to a biological experiment device technical field especially involves an one -way sampler. Including first silicone tube, be provided with a gaseous sterilization filter, an unidirectional valve. The second silicone tube, through the check valve is connected first silicone tube, syringe needle extrusion sampling probe connects the second silicone tube. The utility model discloses one -way sampler is used for connecting the exit end in the sample cushion flask, and the aseptic cell culture liquid in the cushion flask of will taking a sample comes out through the one -way extraction of one -way sampler, aseptic cell culture liquid that has reduced in the sample cushion flask effectual like this and external direct contact's risk, and the success rate of cell culture technology has been guaranteed to the at utmost.

Description

A kind of unidirectional sample device

Technical field

This utility model relates to biotic experiment equipment technical field, refers more particularly to a kind of unidirectional take Sample device.

Background technology

The technological requirement of cell culture technology is relatively stricter, its main and other common laboratory works That makees differs primarily in that requirement keeps sterile working completely, it is to avoid microorganism and extraneous other The impact of harmful factor.The pollution behave that prevents of cell culture technology is to determine that cell cultivates success or failure Key, even if well-appointed laboratory, if experimenter is careless, operating technology is not Specification, also results in pollution, therefore all operations will the guarantee of maximum possible aseptic.Such as: When using sampling injector directly to extract steril cell culture fluid in airtight bioreactor so that Aseptic cell culture fluid directly contacts with external environment, easily causes germ contamination, thus causes The failure of whole cell culture process Development Engineering.

Utility model content

In view of above-mentioned technical problem defect, this utility model provides a kind of unidirectional sample device, should Unidirectional sample device is primarily to avoid sampling operation in cell culture process development process easily to make Become the germ contamination of cell culture fluid in bioreactor, at utmost ensure cell culture process Success rate.

The technical scheme that this utility model is used for above-mentioned technological deficiency is:

A kind of unidirectional sample device, it is characterised in that described unidirectional sample device includes:

First silica gel tube, is provided with a gas sterilizing filter；

Check valve；

Second silica gel tube, connects described first silica gel tube by described check valve；

Syringe needle extruding sampling head, connects described second silica gel tube.

As preferably, above-mentioned unidirectional sample device, it is characterised in that described syringe needle extruding sampling Thief hole floating packing it is provided with on Tou.

As preferably, above-mentioned unidirectional sample device, it is characterised in that also include protection cap, use In sealing described syringe needle extruding sampling head.

Technique scheme has the advantage that or beneficial effect:

The utility model discloses a kind of unidirectional sample device, this unidirectional sample device is used for being connected to take The port of export of sample surge flask, by the steril cell culture fluid in sampling surge flask through unidirectional sample Device is unidirectional to be extracted, and so effectively reduces the steril cell culture fluid in sampling surge flask The risk directly contacted with the external world, at utmost ensure that the success rate of cell culture process.

Accompanying drawing explanation

The detailed description with reference to the following drawings, non-limiting example made by reading, this reality Will become more apparent by novel and feature, profile and advantage.In whole accompanying drawings identical The labelling identical part of instruction.Can not be drawn to scale accompanying drawing, it is preferred that emphasis is illustrate Purport of the present utility model.

Fig. 1 is the structural representation of unidirectional sample device in this utility model embodiment.

Detailed description of the invention

With specific embodiment, this utility model is further described below in conjunction with the accompanying drawings, but It is not as restriction of the present utility model.

This utility model uses sampling injector directly to extract airtight bioreactor in view of tradition During middle steril cell culture fluid so that aseptic cell culture fluid directly contacts with external environment, Easily cause germ contamination, thus cause the failure of whole cell culture process Development Engineering.Therefore, Specially a kind of unidirectional sample device is provided, at utmost to ensure the success of cell culture process Rate.

The structural representation of unidirectional sample device in concrete this new embodiment as shown in Figure 1, This unidirectional sample implement body includes:

First silica gel tube 2, this first silica gel tube is provided with a gas sterilizing filter 1.

Check valve 3.

Second silica gel tube 4, connects the first silica gel tube 2 by check valve 3, and wherein, this is unidirectional In valve work process, its Cell sap flow direction is the direction that the first silica gel tube points to the second silica gel tube.

Syringe needle extruding sampling head 5, connects described second silica gel tube 4, i.e. syringe needle extruding sampling head It is connected on check valve by the second silica gel tube.

In embodiment of the present utility model, it is preferred that be provided with on syringe needle extruding sampling head and take Sample hole floating packing (not shown), when unidirectional sample device does not works at ordinary times, this sampling Hole floating packing is by sealing syringe needle extruding sampling head to avoid external environmental；The most also Including protection cap 6, it is used for sealing syringe needle extruding sampling head, and also seals thief hole movable sealing Pad.

Gas sterilizing filter, check valve, syringe needle, in sampling process, are squeezed by this utility model Pressure sampling is first-class to be attached as shown in figure by silica gel tube, carries out irradiation sterilization after connecting. Occupation mode is as follows: under super-clean bench, with sampling surge flask, the first silica gel tube is carried out aseptic company Connect, the daily sampling operation of reactor can be carried out after completing: open protection cap, use spiral mouth Syringe is connected with syringe needle extruding sampling head, twitches spiral mouth syringe so that sampling surge flask In Cell sap sequentially pass through gas sterilizing filter, the first silica gel tube, check valve, the second silicon Sebific duct and syringe needle extruding sampling head enter in spiral mouth syringe and complete sampling operation, and this sets Meter with sampling surge flask with the use of, sampling operation is the most unidirectional to be carried out, in spiral mouth syringe Cell sap cannot reflux, thus effectively reduce the risk of germ contamination.

In sum, the utility model discloses a kind of unidirectional sample device, this unidirectional sample device is used In being connected to sample the port of export of surge flask, by the steril cell culture fluid warp in sampling surge flask Crossing that unidirectional sample device is unidirectional to be extracted, so effectively reduce in sampling surge flask is aseptic The risk that cell culture fluid directly contacts with the external world, at utmost ensure that cell culture process Success rate.

It should be appreciated by those skilled in the art that those skilled in the art combine prior art and Above-described embodiment can realize described change case, does not repeats at this.Such change case is not Affect flesh and blood of the present utility model, do not repeat them here.

Above preferred embodiment of the present utility model is described.It is to be appreciated that this Utility model is not limited to above-mentioned particular implementation, the equipment that do not describes in detail the most to the greatest extent and Structure is construed as being practiced with the common mode in this area；Any it is familiar with this area Technical staff, without departing under technical solutions of the utility model ambit, may utilize above-mentioned Technical solutions of the utility model are made many possible variations by the method disclosed and technology contents And modification, or it being revised as the Equivalent embodiments of equivalent variations, this has no effect on of the present utility model Flesh and blood.Therefore, every content without departing from technical solutions of the utility model, according to this reality And repair any simple modification made for any of the above embodiments, equivalent variations by novel technical spirit Decorations, all still fall within the range of technical solutions of the utility model protection.

Claims (3)

1. a unidirectional sample device, it is characterised in that described unidirectional sample device includes:

First silica gel tube, is provided with a gas sterilizing filter；

Check valve；

Second silica gel tube, connects described first silica gel tube by described check valve；

Syringe needle extruding sampling head, connects described second silica gel tube.

Unidirectional sample device the most according to claim 1, it is characterised in that be provided with thief hole floating packing on described syringe needle extruding sampling head.

Unidirectional sample device the most according to claim 1, it is characterised in that also include protection cap, is used for sealing described syringe needle extruding sampling head.