CN207408406U - A kind of experimental provision of the sterile degradation environmental contaminants of submerged plant - Google Patents
A kind of experimental provision of the sterile degradation environmental contaminants of submerged plant Download PDFInfo
- Publication number
- CN207408406U CN207408406U CN201720996360.0U CN201720996360U CN207408406U CN 207408406 U CN207408406 U CN 207408406U CN 201720996360 U CN201720996360 U CN 201720996360U CN 207408406 U CN207408406 U CN 207408406U
- Authority
- CN
- China
- Prior art keywords
- glass
- glass bottle
- sterile
- submerged plant
- bottle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 230000015556 catabolic process Effects 0.000 title claims abstract description 23
- 238000006731 degradation reaction Methods 0.000 title claims abstract description 23
- 239000000356 contaminant Substances 0.000 title claims abstract description 15
- 230000007613 environmental effect Effects 0.000 title claims abstract description 14
- 239000011521 glass Substances 0.000 claims abstract description 78
- 239000003344 environmental pollutant Substances 0.000 claims abstract description 21
- 231100000719 pollutant Toxicity 0.000 claims abstract description 21
- 239000012528 membrane Substances 0.000 claims abstract description 12
- 238000007789 sealing Methods 0.000 claims abstract description 11
- 238000005070 sampling Methods 0.000 claims abstract description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 10
- 239000011148 porous material Substances 0.000 claims description 7
- 229910052742 iron Inorganic materials 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims 1
- 244000005700 microbiome Species 0.000 abstract description 14
- 238000011160 research Methods 0.000 abstract description 7
- 235000015097 nutrients Nutrition 0.000 abstract description 3
- 230000008635 plant growth Effects 0.000 abstract description 3
- 238000000746 purification Methods 0.000 abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 20
- 230000000694 effects Effects 0.000 description 7
- 239000012531 culture fluid Substances 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 239000008223 sterile water Substances 0.000 description 5
- XLYOFNOQVPJJNP-ZSJDYOACSA-N Heavy water Chemical compound [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 229910001385 heavy metal Inorganic materials 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 230000000593 degrading effect Effects 0.000 description 2
- 230000009977 dual effect Effects 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 239000003673 groundwater Substances 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000002352 surface water Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Landscapes
- Sampling And Sample Adjustment (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a kind of experimental provisions of the sterile degradation environmental contaminants of submerged plant, glass bottle neck is equipped among cylindrical glass bottle top, one side position of glass bottle neck sets the one-way glass perforate of a connecting needle filter, and entrance is added as pollutant；The top one side of cylindrical glass bottle bottom of bottle sets the two-way glass perforate of a connecting needle filter and rubber hose, as sample tap；Glass bottle opening in glass bottle neck is sealed with sterile breathable sealing film, is fixed with the rubber band cerclage of fixed filter membrane, as gas exchanges mouth.The utility model has the advantages that following prominent：The influence of environmental microorganism can be excluded, investigates purification and degradation of the submerged plant itself to pollutant；Pollutant can be added at any time, at any time sampling analysis, secondary pollution will not be generated to system；The pollutant kind of nutrient, gas exchanges and research needed for submerged plant growth, can be simulated according to actual needs.
Description
Technical field
It is specially that a kind of submerged plant is degraded environmental pollution in an aseptic environment the utility model is related to field of environment engineering
The experimental provision of object.
Background technology
In recent years, with the fast development of China's economy, the surface water bodies such as river, lake, reservoir or even body of groundwater are general
All over being contaminated, the restoration of the ecosystem of polluted-water has become China when the important topic that faced in period the last period.Heavy water is planted
For object since complete stool is all lived in water body, stem, leaf, epidermis have absorption as root, make it to pollutant in water body
The effects that absorbing, be enriched with, degrading is apparent.Therefore, the research of Water Ecological Recovery purification polluted water body is carried out using submerged plant
Receive focus of attention.
Water, phosphorus be body eutrophication principal element and plant growth needed for a great number of elements.Therefore,
Submerged plant has good reduction effect to the nutrient in water environment.The absorption of submerged plant and absorption, also may be used
With heavy metal element excessive in Transport And Transformation water environment, Heavy Metals in Waters content is reduced, and the growth of submerged plant is in itself
It is also required to absorb some heavy metal elements to maintain its vital movement.Research is it is also shown that submerged plant can be enriched with degradation environment
Incretion interferent, environmental persistence organic pollution etc..
Since in actual environment, submerged plant is difficult to and microorganism and other environment the degradation removal effect of pollutant
The influence of the factor is effectively peeled off, thus can not illustrate submerged plant in itself to the degradation removal effect of pollutant.At present, testing
Usually degradation is coexisted in submerged plant and simulating pollution object by simulated experiment of the submerged plant carried out in room to contaminant degradation
System, observation wherein pollutant change with time situation, eliminate other water plants, swim animal and plant, water body physics and chemistry
The influence to contaminant degradation such as matter, but influence of the microorganism to system of degrading also is difficult to control or excludes, and this still cannot be clear
Effectively submerged plant is illustrated in itself to the degradation of pollutant.
Utility model content
The purpose of this utility model is that for practical problem in the prior art, it is contemplated that submerged plant degradation is dirty
Contaminate object easily in by environment the microorganism of generally existing influenced, provided for submerged plant degradation of contaminant a kind of by environmental microorganism
It influences to foreclose, only reflect submerged plant in itself to the experimental provision of contaminant degradation effect.
The utility model mainly considers to influence sterile degradation according to the actual conditions of the sterile degradation of contaminant of submerged plant
Following several respects factor：1）Whether submerged plant brings microorganism into itself；2）Whether the simulating pollution object of addition brings microorganism into；
3）Whether reaction system can be exposed in microbial environment during sampling；4）The need that submerged plant survives in system to gas exchanges
It asks, and the analogue experiment installation established.
To achieve the above object, the technical solution adopted in the utility model is as follows：A kind of submerged plant is in an aseptic environment
The experimental provision of degradation environmental contaminants, mainly includes cylindrical glass bottle, glass bottle neck, sterile breathable sealing film, fixation
The two-way glass of the one-way glass perforate of the rubber band, connecting needle filter of filter membrane, connecting needle filter and rubber hose
Perforate；It is characterized in that：Glass bottle neck is equipped among cylindrical glass bottle top, one side position of glass bottle neck sets a company
The one-way glass perforate of syringe filters is connect, entrance is added as pollutant；The top one side of cylindrical glass bottle bottom of bottle is set
The two-way glass perforate of a connecting needle filter and rubber hose is put, as sample tap；Glass bottle opening in glass bottle neck
It is sealed, is fixed with the rubber band cerclage of fixed filter membrane, as gas exchanges mouth with sterile breathable sealing film.
Cylindrical glass bottle described in the utility model, bottom of bottle diameter 15cm, the high 20cm of body without glass bottle neck, bottle
Body volume about 3.5L, body indicate body graduation mark, the high 8cm of glass bottle neck, glass bottle opening diameter 8cm.In sterile room,
A certain amount of sterile water or aseptic culture fluid can be added in vial by glass bottle opening, and will sterilized treated sinks water
Plant is fitted into vial.The glass body size can need to customize according to practical study.
Glass bottle neck described in the utility model adds in submerged plant and sterile water in sterile room from its bottleneck
（Aseptic culture fluid）Afterwards, sealed immediately with sterile breathable sealing film, sealed membrane is fixed with rubber band cerclage.
The one-way glass perforate of connecting needle filter described in the utility model is opened apart from glass bottle neck 2.5cm positions
Hole, open pore size are internal diameter 4mm, outer diameter 7mm, carry out closure setting with 0.22 μm of syringe filters thereon；Both can play every
The effect of exhausted microorganism, and experiment syringe and 0.22 μm can be passed through after submerged plant adaptability grows a period of time
Syringe filters upper end is connected, and adds the simulated environment pollutant solution of configuration at any time, plays addition pollutant and impurity screening
With the effect of microorganism.
The two-way glass perforate of connecting needle filter described in the utility model and rubber hose is apart from bottle bottom 5cm
The hole put out, open pore size are internal diameter 4mm, outer diameter 7mm, and a bottle inside carries out closure setting, bottle with 0.22 μm of syringe filters
Outside is connected with rubber hose, is sealed with iron clamp, plays a dual role of completely cutting off microorganism and sample tap.
The filter membrane diameter of 0.22 μm of syringe filters described in the utility model, material can need to customize purchase according to research
It buys, selects water system or organic system, the intensity of filter cross-section that can bear maximum 20cm's according to the property of studied pollutant
Water column pressure.Lower interface is standard medical needles cylinder socket in 0.22 μm of syringe filters, can be with conventional medical socket pin
Cylinder is connected.Interface thereon（Connect syringe end）Outer diameter is about 6.5mm, and internal diameter is about 4.4mm；Lower interface（Connect needle end）Outer diameter is about
For 4mm, internal diameter is about 2.2mm.
Sterile breathable sealing film described in the utility model is 16cm × 16cm, and 0.2-0.3 μm of pore size filter, transparency exists
More than 90%, there are airing function, high temperature resistant sterilizing.Sealed membrane can ensure air exchange without having the entrance such as dust and mould
It is fungi-proofing so as to achieve the purpose that seal in bottle.Sealed membrane size can be customized according to the actual size of glass bottle opening and bought.
The utility model has the advantages that following prominent：1）The influence of environmental microorganism can be excluded, investigates submerged plant certainly
Purification and degradation of the body to pollutant；2）Pollutant can be added at any time, at any time sampling analysis, two will not be generated to system
Secondary pollution；3）The pollutant kind of nutrient, gas exchanges and research needed for submerged plant growth, can be according to reality
It is simulated, it is highly practical.
Description of the drawings
Fig. 1 is the Experimental equipment of the utility model.
Fig. 2 is the bottleneck top view of the utility model.
Critical piece symbol description in attached drawing：1st, cylindrical glass bottle；2nd, glass bottle neck；3rd, sterile breathable sealing film；
4th, the rubber band of fixed filter membrane；5th, 0.22 μm of syringe filters；6th, the one-way glass perforate of connecting needle filter；7th, body is carved
Spend line；8th, glass bottle opening；9th, the two-way glass perforate of connecting needle filter and rubber hose；10th, iron clamp；11st, rubber hose；
12nd, submerged plant；13rd, sterile water or aseptic culture fluid.
Specific embodiment
Referring to Fig. 1-2, the technical solution adopted in the utility model is as follows：A kind of submerged plant is degraded ring in an aseptic environment
The experimental provision of border pollutant mainly includes cylindrical glass bottle 1, glass bottle neck 2, sterile breathable sealing film 3, fixed filter membrane
Rubber band 4, the one-way glass perforate 6 of connecting needle filter, the two-way glass of connecting needle filter and rubber hose opens
Hole 9；It is characterized in that：Glass bottle neck 2 is equipped among 1 top of cylindrical glass bottle, 2 one side position of glass bottle neck sets one
The one-way glass perforate 6 of connecting needle filter adds entrance as pollutant；The top one of 1 bottom of bottle of cylindrical glass bottle
Side sets the two-way glass perforate 9 of a connecting needle filter and rubber hose, as sample tap；Glass in glass bottle neck 2
Glass bottleneck 8 is sealed with sterile breathable sealing film 3, is fixed with 4 cerclage of rubber band of fixed filter membrane, as gas exchanges mouth.
Cylindrical glass bottle 1 described in the utility model, bottom of bottle diameter 15cm, the high 20cm of body without glass bottle neck 2,
Bottle volume about 3.5L, body indicate body graduation mark 7, the high 8cm of glass bottle neck, glass bottle opening diameter 8cm；The connecting needle
The one-way glass perforate 6 of filter is the hole opened apart from glass bottle neck 2.5cm positions, and open pore size is internal diameter 4mm, outer diameter 7mm,
Thereon closure setting is carried out with 0.22 μm of syringe filters；Not only it can play the role of completely cutting off microorganism, but also can be planted in heavy water
After object adaptability growth a period of time, it is connected by experiment with syringe with 0.22 μm of 5 upper end of syringe filters, addition at any time is matched somebody with somebody
The simulated environment pollutant solution put plays the role of adding pollutant and impurity screening and microorganism；The connecting needle mistake
The hole that the two-way glass perforate 9 of filter and rubber hose is opened apart from bottle bottom 5cm positions, open pore size be internal diameter 4mm, outer diameter
7mm, a bottle inside carry out closure setting with 0.22 μm of syringe filters 5, and bottle outside rubber hose 11 connects, with 10 envelope of iron clamp
Mouthful, play a dual role of completely cutting off microorganism and sample tap.
The required submerged plant 12 of experiment is gathered, first with tap water purifying culture 3-5 days, tests and flowing is used before starting certainly
Water rinse repeatedly 2 it is small when, impregnate 5min with 70% alcohol immersion treatment 10-20 seconds, then with 1%NaClO, it is finally anti-with sterile water
5min is rinsed again, is put into the vial of sterilized processing, while according to research needs, the nothing of addition certain volume into bottle
Bacterium water or aseptic culture fluid 13.
For glass body when carrying out sterilization treatment, glass perforate is with 0.22 μm of syringe filters closure fixation, use at two
Iron clamp clamps rubber tube, and bottleneck ordinary filter paper adds the double-deck covering of brown paper to fix, is integrally put into high-pressure sterilizing pot, 125 DEG C go out
Bacterium 30 minutes.It stays in and submerged plant and sterile water has been added in gnotobasis（Aseptic culture fluid）Afterwards, glass bottle opening is used instead through going out
The sterile breathable sealing film and rubber band of bacterium are fixed, you can are transferred to carry out in the environment needed for research by experimental provision and be tested.
This patent is that a kind of submerged plant of utility model is degraded the experimental provisions of environmental contaminants in an aseptic environment.The mould
Intending device can be as the experimental provision for carrying out the sterile degradation environmental contaminants of submerged plant.This simulator has practicability
By force, it is widely used, is at low cost, the features such as simulated conditions are suitable, strong operability, there is good application and popularization value.
Claims (4)
1. a kind of experimental provision of the sterile degradation environmental contaminants of submerged plant, mainly includes cylindrical glass bottle, vial
Neck, sterile breathable sealing film, fixed filter membrane rubber band, connecting needle filter one-way glass perforate, connecting needle filtering
The two-way glass perforate of device and rubber hose；It is characterized in that：Glass bottle neck, glass are equipped among cylindrical glass bottle top
One side position of bottleneck sets the one-way glass perforate of a connecting needle filter, and entrance is added as pollutant；Cylindrical glass
The top one side of glass bottle bottom of bottle sets the two-way glass perforate of a connecting needle filter and rubber hose, as sampling
Mouthful；Glass bottle opening in glass bottle neck is sealed with sterile breathable sealing film, is fixed, made with the rubber band cerclage of fixed filter membrane
For gas exchanges mouth.
2. a kind of experimental provision of the sterile degradation environmental contaminants of submerged plant according to claim 1, it is characterised in that：
The cylindrical glass bottle, bottom of bottle diameter 15cm, the high 20cm of body without glass bottle neck, bottle volume about 3.5L, body
Indicate body graduation mark, the high 8cm of glass bottle neck, glass bottle opening diameter 8cm.
3. a kind of experimental provision of the sterile degradation environmental contaminants of submerged plant according to claim 2, it is characterised in that：
The one-way glass perforate of the connecting needle filter is the hole opened apart from glass bottle neck 2.5cm positions, and open pore size is internal diameter
4mm, outer diameter 7mm carry out closure setting with 0.22 μm of syringe filters thereon.
4. a kind of experimental provision of the sterile degradation environmental contaminants of submerged plant according to claim 3, it is characterised in that：
The hole that the two-way glass perforate of the connecting needle filter and rubber hose is opened apart from bottle bottom 5cm positions, open pore size
For internal diameter 4mm, outer diameter 7mm, bottle inside carries out closure setting with 0.22 μm of syringe filters, is connected on the outside of bottle with rubber hose
It connects, is sealed with iron clamp.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720996360.0U CN207408406U (en) | 2017-08-10 | 2017-08-10 | A kind of experimental provision of the sterile degradation environmental contaminants of submerged plant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720996360.0U CN207408406U (en) | 2017-08-10 | 2017-08-10 | A kind of experimental provision of the sterile degradation environmental contaminants of submerged plant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207408406U true CN207408406U (en) | 2018-05-25 |
Family
ID=62322797
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720996360.0U Expired - Fee Related CN207408406U (en) | 2017-08-10 | 2017-08-10 | A kind of experimental provision of the sterile degradation environmental contaminants of submerged plant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207408406U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110476648A (en) * | 2019-08-02 | 2019-11-22 | 江南大学 | A kind of root-pouring method inoculated plant endophyte experiment sterile culture device |
-
2017
- 2017-08-10 CN CN201720996360.0U patent/CN207408406U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110476648A (en) * | 2019-08-02 | 2019-11-22 | 江南大学 | A kind of root-pouring method inoculated plant endophyte experiment sterile culture device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US10645886B2 (en) | Methods and apparatus for gnotobiotic plant growth | |
| CN105670916B (en) | Aseptic packaging interlayer sterility test totally-enclosed membrane filter and its detection method | |
| CN207408406U (en) | A kind of experimental provision of the sterile degradation environmental contaminants of submerged plant | |
| CN112322468A (en) | Unidirectional negative pressure adsorption sampling and filtering device and method | |
| Chang et al. | Viability and Destruction of the Cysts of Endameba histolytica | |
| CN107058074B (en) | A kind of packaged type microorganism classification sampling, culture and detection integrated apparatus | |
| Javed et al. | Enhancing waterborne diseases in Pakistan & their possible control | |
| CN101962231B (en) | Biological microbial inoculum culture and adding device for in-situ remediation of micro-polluted water body | |
| CN101921045B (en) | Urine purifying device | |
| CN113664033A (en) | Method for removing heavy metal pollution of flooded soil | |
| CN211078756U (en) | Portable water purification equipment for water pumping engineers in water resource pollution area | |
| CN107179223B (en) | Root surface iron film/microbial film generation simulation device and method | |
| CN203904123U (en) | Novel ultrapure water system | |
| CN203148726U (en) | Sterile sampling bottle | |
| CN205473760U (en) | Sample cushion flask | |
| CN206083400U (en) | Absorption ceramic pipe of multiple hole soil heavy metal | |
| CN215492690U (en) | Plant root secretion and plant volatile gas continuous collecting and culturing device | |
| CN103194381A (en) | Screening and identification kit of denitrifying bacteria and screening and identification method | |
| CN208762224U (en) | A kind of closed aeration biochemical reaction experiment device | |
| CN204999898U (en) | VOCs increases bacteriolytic open fermentation cultivation device | |
| CN205473764U (en) | Biological reaction bag sample pipeline | |
| CN209443001U (en) | A kind of microorganism count device | |
| Mouhamad et al. | Detect of human fecal contamination in water and soil of multiple sanitary landfills in Baghdad city | |
| CN208327666U (en) | A kind of self-priming outdoor purifier | |
| CN205473765U (en) | One -way sampler |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180525 Termination date: 20190810 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |