CN106932241A - A kind of method of culture soil greenhouse gas emission in measuring cell - Google Patents
A kind of method of culture soil greenhouse gas emission in measuring cell Download PDFInfo
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- CN106932241A CN106932241A CN201710071058.9A CN201710071058A CN106932241A CN 106932241 A CN106932241 A CN 106932241A CN 201710071058 A CN201710071058 A CN 201710071058A CN 106932241 A CN106932241 A CN 106932241A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/22—Devices for withdrawing samples in the gaseous state
- G01N1/2226—Sampling from a closed space, e.g. food package, head space
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/22—Devices for withdrawing samples in the gaseous state
- G01N1/24—Suction devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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Abstract
The present invention relates to a kind of method of culture soil greenhouse gas emission in measuring cell, the inventive method is carried out using ventilation culture gas production integrated apparatus, the culture gas producing device that described ventilation culture gas production integrated apparatus include air interchanger and are connected with air interchanger；Assay method includes the open culture of soil, ventilation, closed culture, gas collecting, this method eliminates influence of the gas of early stage soil generation to closed culture gas discharge, realize closed culture under normal pressure, the absorption problem for overcoming sampling to cause negative pressure to produce object gas simultaneously, and can once complete culture and the gas collecting of multiple blake bottles, initial incubation concentration is consistent, high working efficiency.
Description
Technical field
The present invention relates to a kind of method of culture soil greenhouse gas emission in measuring cell, belong to Greenhouse research
Technical field.
Background technology
The increase of atmospheric greenhouse gas concentration is the main motivation of global warming, carbon dioxide (CO2), first
Alkane (CH4) and nitrous oxide (N2O it is) 3 kinds of most important greenhouse gases in air, their contribution rates to global warming account for closely
80%, and its atmospheric concentration increases with 0.5%, 0.8% and 0.3% average annual speed respectively.In all greenhouse gas emission sources
In, soil is most important emission source, it is estimated that, there is the CO of 5%-20% in air every year2, 15%-30% CH4And 80%-
90% N2O derives from soil.Therefore, 3 kind main greenhouse gas discharge of the domestic and foreign scholars to Different ecosystems soil is opened
Dependent observation has been opened up with research.
Main at present to carry out in-situ observation in the wild using case method and microclimate method, the day for studying greenhouse gas emission becomes
Change, seasonal and annual variation rule, and estimate annual discharge capacity.Due to the complexity of wild environment, unpredictability and experiment
The limitation of condition, in addition it is also necessary to tested by the controllable indoor soil incubation of conjugation condition, to the environmental Kuznets Curves factor or specific feelings
The discharge of greenhouse gases conducts a research under scape pattern.The experiment of indoor soil incubation be it is quantitative and understand in depth gas emission mechanism and
The most powerful measure of influence factor, is also the effective supplement to Field observational experiment.It is many using between indoor soil in research at present
The closed culture of formula of having a rest takes gas method, and the method is fitted into blake bottle after a certain amount of pedotheque is adjusted according to test requirements document,
Being placed under certain environment carries out open culture, periodically by the moisture of gravimetric method supplement evaporation loss, by short time closed training
Support, gather gas in closed preceding and closed rear blake bottle, gas content is determined using gas chromatography, carried out again after sample collection
Open culture is until collection next time gaseous sample.For ease of the indoor culture of batch, many glass using small volume in experiment
Bottle as blake bottle, such as triangular flask of 250ml or the aseptic serum bottles of 100ml, if operated according to the method described above, may
There is the problem of interference culture soil gas discharge, so as to influence the accuracy of experimental result.Influencing factor mainly has following several
Point：1) because culture bottleneck is smaller, the gas of soil incubation release should not shed, and cause gas concentration to increase and influence culture period
Between gas discharge.2) in closed incubation, the gas of soil release can cause gas pressure in culture apparatus to be higher than air
Pressure, it is impossible to keep the natural concentration gradient of soil-inferior atmospheric layer interface, the result for drawing to be difficult to describe the real gas of soil
Release.3) cultivated with vial, if sampling quantity is excessive, negative pressure in blake bottle can be caused and pedotheque occurred and absorbed gas
Phenomenon.4) gather gas sample before closed and after closed to be analyzed, workload is big, and analysis cost is higher.5) it is multiplex in testing
Gas is taken in fine needle insertion silica gel plug, syringe needle is easily caused and is blocked and long-term use silica gel plug gas leakage.
There is presently no it is a kind of cultivate it is more authentic and valid, closer in culture soil greenhouse gases row in actual measuring cell
The disclosure of the method pertinent literature put.Therefore, it is necessary to set up a kind of science, be suitable to indoor culture soil greenhouse gas emission
Assay method.
The content of the invention
In view of the shortcomings of the prior art, the method that the present invention provides culture soil greenhouse gas emission in a kind of measuring cell,
Influence of the gas of early stage soil generation to closed culture gas discharge is this method eliminates, closed culture under normal pressure is realized, together
When overcome the absorption problem that sampling causes negative pressure to produce object gas, and can once complete the culture of multiple blake bottles with
Gas collecting, it is ensured that initial incubation concentration is consistent, improves greenhouse gases and determines operating efficiency.
The present invention is achieved through the following technical solutions：
A kind of method of culture soil greenhouse gas emission in measuring cell, the method is disguised using ventilation culture gas production one
Putting is carried out, the culture gas production dress that described ventilation culture gas production integrated apparatus include air interchanger and are connected with air interchanger
Put；
Described culture gas producing device, including upper end open blake bottle, sealing-plug, sealing-plug are provided with upper end open
It is upper that air admission hole and gas production hole are set, air inlet pipe is fixed with air admission hole, in air inlet pipe one end insertion blake bottle, the other end is connected with
Air inlet two-port valve, the upper port connection balance pipe of air inlet two-port valve fixes air collecting pipe, the insertion culture of air collecting pipe one end in gas production hole
In bottle, the other end is connected with gas production two-port valve, the upper port connection gas production needle tubing of gas production two-port valve；
Described air interchanger includes ventilation house steward, and a port of the house steward that takes a breath is connected by leather hose with aspiration pump, separately
One end is closed, and ventilation house steward is fixed on support, and the branch pipe being connected with ventilation house steward, branch pipe are arranged at intervals with ventilation house steward
On be provided with gas opening and closing device, the bottom of branch pipe is connected with connecting tube, the gas production two-port valve of connecting tube and culture gas producing device
Connection；
Assay method includes the open culture of soil, ventilation, closed culture, gas collecting, and step is as follows：
(1) the open culture of soil
The soil sample that will be handled well is put into the blake bottle of culture gas producing device, is covered sealing-plug and is sealed, by air inlet two-way
Valve and gas production two-port valve are opened and ventilated with extraneous, keep freely being ventilated inside and outside bottle, carry out aerobic culture, supplemented in incubation because
The moisture of evaporation loss, reaches consistent with the gross weight before culture；
(2) take a breath
The gas production two-port valve and connecting tube of connection culture gas producing device, keep air inlet two-port valve open mode, adjust gas production
Gas opening and closing device on two-port valve and air interchanger branch pipe, makes blake bottle be communicated with air interchanger；Open pumping switch pump pair
Blake bottle is evacuated, and makes gas in blake bottle suitable with the external world；Aspiration pump is then shut off, gas production two-port valve, air inlet two is simultaneously closed off
Port valve, makes blake bottle be in air-tight state, gathers ambient atmos, and ambient atmos concentration is the initial concentration before culture；
(3) closed culture
Gas production two-port valve and connecting tube are disconnected, balance pipe is emptied, balance pipe is connected with air inlet two-port valve, open air inlet two
Port valve, makes balance pipe be communicated with blake bottle, keeps air pressure in blake bottle；According to test requirements document, whole device is placed on corresponding bar
Closed culture 1-24h under part；
(4) gas collecting
At the end of closed culture, gas production needle tubing is connected with gas production two-port valve, opens gas production two-port valve, repeatedly push-and-pull gas production
Needle tubing, fully to mix gas in blake bottle, gathers gas immediately.
Currently preferred, after gas collecting is finished, repeat step (2) excludes the gas that soil incubation is produced in blake bottle
After body, open culture is continued according still further to step (1), the sampling of different time sections is realized in repeat step (2)-(4).
Soil that is currently preferred, being handled well in the step (1), can be by water content, nitrogen concentration, pH value,
The soil of the different disposals such as cultivation temperature；Soil quality culture apparatus according to used by determines.
Currently preferred, step (1) weighs and records the gross weight of soil sample and blake bottle, in incubation before culture
Supplemented because of the moisture of evaporation loss per 2-4d with gravimetric method, using the syringe with elongated syringe needle by two-port valve in blake bottle
Supplement distilled water, reaches consistent with the gross weight of record before culture.
According to currently preferred, described culture gas producing device：
Described sealing-plug is silica gel plug, and silica gel plug diameter is consistent with bottleneck diameter to be advisable.
The distance between described air inlet pipe bottom and culture soil top layer are the 1/8-1/5 of blake bottle height.Reduce into
Enter disturbance of the gas to soil.
Described air collecting pipe bottom is sealing-plug bottom and culture soil top layer distance height with the distance of sealing-plug bottom
1/5-1/4.
Described blake bottle is the Flat bottom containers such as triangular flask, wide-mouth bottle or narrow-mouthed bottle, and material may be selected plastics or glass.
Described air inlet pipe and air collecting pipe are polyfluortetraethylene pipe, a diameter of 2-2.5mm, with close with the thin mouth end of two-port valve
Close connection to be advisable, the preferred 0.8-1.2mm of internal orifice dimension.
Described two-port valve carries valve switch, the closure state for controlling different port.
The light smoothly plastic injector of the preferred piston pull of described balance pipe, to facilitate described device in closed culture
Under state, partial pressure is carried out to the gas that culture soil is produced, to keep air pressure in blake bottle, the preferred 10ml-20ml of capacity.
It is currently preferred, to prevent causing negative pressure in pumping process, the discharge of gas is influenceed, gas production pin when mixing gas
The push-and-pull degree of pipe and the gas volume for gathering, no more than gas volume in balance pipe.
Culture gas producing device should be at air-tight state after the two-port valve port shutdown parallel with blake bottle.
According to currently preferred, described air interchanger：
Described ventilation house steward is hollow pipe, and hollow pipe includes two parallel straight tubes and two parallel vertical tubes, and two parallel
Vertical tube is located between two parallel straight tubes and connection forms loop, and branch pipe equi-spaced apart is arranged on two parallel straight tubes, two
Branch pipe on parallel straight tube intersects alternate setting, in order to place culture gas producing device, once completes the ventilation of multiple blake bottles.
Described branch pipe quantity is 10-20, adjacent branch interval 10cm-20cm.
Described gas opening and closing device is preferably piston.
The material of hollow pipe of the invention, branch pipe and piston is glass；The material of leather hose and connecting tube is hollow hard
Plastics, plastic pipe deformation when preventing from being evacuated.
Two parallel straight tubes extend to two ends connection end is formed outside vertical tube respectively, and hollow pipe is fixed on branch by connection end
On frame.
The more preferred scheme of the present invention, hollow pipe is fixed on support by flask holder and cross clamp, on flask holder
Set screw is provided with, for clamping hollow pipe, flask holder is fixed on cross clamp by set screw, cross clamp passes through roll adjustment
Screw is fixed on support.
According to currently preferred, time of exchanging gas is 30s-3min.Ventilation is dense according to gas buildup in blake bottle volume, bottle
Spend to consider with the speed of exhaust and be adjusted, to meet the gas for excluding that early stage culture is produced in blake bottle, make in blake bottle
Gas is suitable with the external world to be advisable.
According to currently preferred, closed incubation time is 2-10h, to meet gas buildup concentration and instrument in blake bottle
Detection limit.
Raw materials used and equipment of the invention is prior art.
Advantages of the present invention is as follows：
1st, the apparatus structure that the present invention is provided is simple, easily fabricated, and material is easy to get, cheap, convenient disassembly, is easy to clear
Wash, easy to operate, simulation effect is good, it is adaptable to the culture of lab simulation soil and gas collecting.
2nd, using technical scheme, with gas production needle tubing by the direct gas production of two-port valve, largely avoid
Cause pin hole to block when inserting silicone tube gas production with elongated syringe needle and cause the problem of silica gel plug gas leakage due to insertion needle.
3rd, using technical scheme, purging steps can overcome early stage soil release gas concentration in blake bottle
The problem of object gas discharge during increasing the closed culture of influence, can once complete the ventilation of multiple blake bottles, collection culture
, used as initial incubation gas, initial vapor concentration is consistent, and workload halves for air near bottle.
4th, using technical scheme, it is possible to achieve discharge the partial pressure of gas in closed incubation to soil, protect
Culture under normal pressure is held, and can avoid causing negative pressure to produce the problem for absorbing to object gas during sampling, so as to more be connect
Soil gas discharge under nearly actual state.
In sum, soil incubation of the invention and gas collecting device, with being simple to manufacture, it is with low cost, be easy to it is clear
Wash, it is easy to operate quick the features such as, atmospheric closed culture can be kept using process, and avoid producing negative pressure influence object gas
Discharge, moreover it is possible to improve the advantage of operating efficiency, it is ensured that the quality of simulating lab test.
Brief description of the drawings
Fig. 1 is the structural representation of the air interchanger of the ventilation culture gas production integrated apparatus that the inventive method is used；
Wherein：1st, hollow pipe；2nd, branch pipe；3rd, piston；4th, flask holder；5th, set screw；6th, cross clamp；7th, screw；8th, adjust
Away from screw；9th, support；10th, leather hose；11st, aspiration pump；12nd, connecting tube.
Fig. 2 is that the structure of the culture gas producing device of the ventilation culture gas production integrated apparatus that Fig. 1 the inventive method is used is shown
It is intended to；
Wherein：13rd, blake bottle；14th, sealing-plug；15th, air admission hole；16th, gas production hole；17th, air inlet pipe；18th, air collecting pipe；19、
Two-port valve；20th, balance pipe；21st, gas production needle tubing；22nd, culture sample.
Fig. 3 is the structural representation of the ventilation culture gas production integrated apparatus that the inventive method is used.
Fig. 4 is soil N under the different pH value that the inventive method is determined2O rate of discharge block diagrams.
Specific embodiment
Below by specific embodiment, the present invention will be further described, but not limited to this.
Embodiment 1,
A kind of method of culture soil greenhouse gas emission in measuring cell, the method is disguised using ventilation culture gas production one
Putting is carried out, the culture gas production dress that described ventilation culture gas production integrated apparatus include air interchanger and are connected with air interchanger
Put；
Described culture gas producing device, structure are as shown in Fig. 2 the blake bottle 13 including upper end open, sets at upper end open
Sealing-plug is equipped with, sealing-plug is silica gel plug 14, and silica gel plug diameter is consistent with bottleneck diameter to be advisable, and air admission hole 15 is set on sealing-plug
With gas production hole 16, air inlet pipe 17 is fixed with air admission hole 15, in the one end of air inlet pipe 17 insertion blake bottle 13, air inlet pipe bottom and training
It is the 1/8-1/5 of blake bottle height to support the distance between upper soll layer.Reduce the disturbance for entering gas to soil.The other end is connected
There is air inlet two-port valve 19, the upper port connection balance pipe 20 of air inlet two-port valve 19 fixes air collecting pipe 18, air collecting pipe in gas production hole 16
In 18 one end insertion blake bottle 13, the distance of air collecting pipe bottom and sealing-plug bottom for sealing-plug bottom and culture soil top layer away from
From the 1/5-1/4 of height.The other end is connected with gas production two-port valve 19；The upper port connection gas production needle tubing 21 of gas production two-port valve 19.
Blake bottle is wide-mouth bottle, and air inlet pipe and air collecting pipe are polyfluortetraethylene pipe, a diameter of 2-2.5mm, with the thin mouth end with two-port valve
Airtight connection is advisable, the preferred 0.8-1.2mm of internal orifice dimension.Two-port valve carries valve switch, the closed form for controlling different port
State.The light smoothly plastic injector of the preferred piston pull of balance pipe, to facilitate described device under closed cultivation conditions, to training
The gas for supporting soil generation carries out partial pressure, to keep air pressure in blake bottle, the preferred 10ml-20ml of capacity.Air interchanger, structure is such as
Shown in Fig. 1, including ventilation house steward, take a breath house steward a port be connected with aspiration pump 11 by leather hose 10, take a breath house steward it is another
One end is closed, and ventilation house steward is fixed on support 9, and the branch pipe 2 being connected with ventilation house steward, branch are arranged at intervals with ventilation house steward
Gas opening and closing device is provided with pipe 2, the bottom of branch pipe 2 is connected with connecting tube 12, and connecting tube 12 is adopted with culture gas producing device
Gas two-port valve is connected；
Described ventilation house steward is hollow pipe 1, and hollow pipe 1 includes two parallel straight tubes and two parallel vertical tubes, and two are put down
Row vertical tube is located between two parallel straight tubes and connection forms loop, and the equi-spaced apart of branch pipe 2 is arranged on two parallel straight tubes, and two
Branch pipe on the parallel straight tube of root intersects alternate setting.In order to place culture gas producing device, the training of multiple blake bottles is once completed
Support and gas collecting.
The described quantity of branch pipe 2 is 10-20, adjacent branch interval 10cm-20cm.Gas opening and closing device is preferably piston
3。
The material of hollow pipe, branch pipe and piston is glass；The material of leather hose and connecting tube is hollow rigid plastics, in case
Plastic pipe deformation when being only evacuated.
Two parallel straight tubes extend to two ends connection end is formed outside vertical tube respectively, and hollow pipe is fixed on branch by connection end
On frame.
Hollow pipe is fixed on support by flask holder 4 and cross clamp 6, set screw 5 is provided with flask holder 4, for pressing from both sides
Firmly hollow pipe, flask holder 4 is fixed on cross clamp by set screw 7, and cross clamp is fixed on support by roll adjustment screw 8.
Assay method includes the open culture of soil, ventilation, closed culture, gas collecting, and step is as follows：
(1) the open culture of soil
The 50g soil samples that will be handled well are put into the blake bottle of culture gas producing device, are covered sealing-plug and are sealed, and are weighed and are remembered
Record soil sample and the gross weight of blake bottle, connect air interchanger and culture gas producing device, and air inlet two-port valve and gas production two-port valve are beaten
Open and ventilated with the external world, keep freely being ventilated inside and outside bottle, aerobic culture is carried out under conditions of being placed on 25 DEG C.Weight is used in incubation
Legal phase 2d supplements are distilled by two-port valve with the syringe with elongated syringe needle because of the moisture of evaporation loss to supplement in bottle
Water, reaches consistent with the gross weight of record before culture.
(2) take a breath
The gas production two-port valve and connecting tube of connection culture gas producing device, keep air inlet two-port valve open mode, adjust gas production
Gas opening and closing device on two-port valve, air interchanger branch pipe, makes blake bottle be communicated with air interchanger；Pumping switch pump is opened to training
Bottle pumping 1.5min is supported, makes gas in blake bottle suitable with the external world；Aspiration pump is then shut off, gas production two-port valve is simultaneously closed off, is entered
Gas two-port valve, makes blake bottle be in air-tight state, air near collection blake bottle, and air percent of greenhouse gases concentration is before cultivating
Initial concentration；
(3) closed culture
Gas production two-port valve and connecting tube are disconnected, balance pipe is emptied, balance pipe is connected with air inlet two-port valve, open air inlet two
Port valve, makes balance pipe be communicated with blake bottle, keeps air pressure in blake bottle；According to test requirements document, whole device is placed on 25 DEG C
Under the conditions of closed culture 2h；
(4) gas collecting
At the end of closed culture, with gas production two-port valve be connected gas production needle tubing by two-port valve, opens gas production two-port valve, pushes away repeatedly
Draw gas production needle tubing 3-5 times, fully to mix gas in blake bottle, gas is gathered immediately.
After gas collecting is finished, repeat step (2), after excluding the gas that soil incubation is produced in blake bottle, according still further to step
Suddenly (1) continues open culture, and the sampling of different time sections is realized in repeat step (2)-(4).
Embodiment 2,
A kind of method of culture soil greenhouse gas emission in measuring cell, as described in Example 1, difference is：Training
It is 150ml triangular flasks to support bottle, and the soil sample handled well is 15g, air inlet pipe range 11cm, and the capacity of balance pipe and gas production needle tubing is respectively
10ml and 20ml.Time of exchanging gas is 30s, gas production after 25 DEG C of constant-temperature enclosed culture 4h.
Embodiment 3,
A kind of method of culture soil greenhouse gas emission in measuring cell, as described in Example 1, difference is：Return
Equipped with 10 branch pipes of equidistant intervals on the parallel straight tube of type pipe, the branch pipe intersection on two straight tubes is alternate, is easy to place and trains
Gas producing device is supported, 20 ventilation work of blake bottle at most can be simultaneously completed.
Embodiment 4,
A kind of method of culture soil greenhouse gas emission in measuring cell, as described in Example 1, difference is：Adopt
Collection fresh soil, pH value is 7.5, weighs the soil sample that 50g (in terms of drying soil) crosses 1mm sieves, and pH value is adjusted respectively to 5 and 9.5, each
3 repetitions for the treatment of, after open culture 3d, take gas sample, use gas chromatograph for determination N2The content of O.The difference that the method is determined
Soil N under pH value2O rate of discharges are as shown in figure 4, under the natural ph of soil (7.5), soil discharges N2The ability of O is most strong,
Reach 270.7ng g-1d-1, it is significantly higher than N under the conditions of meta-acid (pH5) or meta-alkali (pH9.5)2The release of O.
Embodiment 5,
A kind of method of culture soil greenhouse gas emission in measuring cell, as described in Example 3, difference is：Adopt
Collection fresh soil, weighs the soil sample that 50g (in terms of drying soil) crosses 1mm sieves, is separately added into nitrogen content (0.1mg g-1Dry ground) it is equal
Various nitrogenous fertilizer：Urea, long-acting carbamide and ammonium sulfate, while a control (without any material) is set, each 3 weight for the treatment of
It is multiple, gas sample is taken respectively at the 1st, 2,3,5 and 10d after fertilising, use gas chromatograph for determination N2The content of O.
Soil N under the different carbon source of table 12O rate of discharges (ng g-1d-1)
As it can be seen from table 1 the nitrogen source of different shape is to N2The influence of O releases is different.N under Urea treatment2The discharge speed of O
Rate is maximum, next to that ammonium sulfate, and long-acting carbamide is to N2The release influence of O is most weak.The lower N of each treatment2The rate of release of O is with culture
The extension of time, in trend is increased, reaches highest in 2d substantially, then gradually reduces, after 5d each treatment substantially with compare one
Cause.Preceding 3d is N from after this result of the test, fertilising2O discharges peak period, and administering long-lasting urea can effectively reduce N2The release of O.
Claims (10)
1. in a kind of measuring cell culture soil greenhouse gas emission method, the method using ventilation culture gas production integrated apparatus
Carry out, the culture gas production dress that described ventilation culture gas production integrated apparatus include air interchanger and are connected with air interchanger
Put；
Described culture gas producing device, including upper end open blake bottle, sealing-plug is provided with upper end open, set on sealing-plug
Air admission hole and gas production hole are put, air inlet pipe is fixed with air admission hole, in air inlet pipe one end insertion blake bottle, the other end is connected with air inlet
Two-port valve, the upper port connection balance pipe of air inlet two-port valve fixes air collecting pipe, air collecting pipe one end insertion blake bottle in gas production hole
Interior, the other end is connected with gas production two-port valve, the upper port connection gas production needle tubing of gas production two-port valve；
Described air interchanger includes ventilation house steward, and a port of the house steward that takes a breath is connected by leather hose with aspiration pump, the other end
Closing, ventilation house steward is fixed on support, and the branch pipe being connected with ventilation house steward is arranged at intervals with ventilation house steward, is set on branch pipe
Gas opening and closing device is equipped with, the bottom of branch pipe is connected with connecting tube, and connecting tube is connected with the gas production two-port valve of culture gas producing device；
Assay method includes the open culture of soil, ventilation, closed culture, gas collecting, and step is as follows：
(1) the open culture of soil
The soil sample that will be handled well is put into the blake bottle of culture gas producing device, is covered sealing-plug and is sealed, by air inlet two-port valve and
Gas production two-port valve is opened and ventilated with extraneous, keeps freely being ventilated inside and outside bottle, carries out aerobic culture, is supplemented in incubation because of evaporation
The moisture of loss, reaches consistent with the gross weight before culture；
(2) take a breath
The gas production two-port valve and connecting tube of connection culture gas producing device, keep air inlet two-port valve open mode, adjust gas production two-way
Gas opening and closing device on valve and air interchanger branch pipe, makes blake bottle be communicated with air interchanger；Pumping switch pump is opened to culture
Bottle pumping, makes gas in blake bottle suitable with the external world；Aspiration pump is then shut off, gas production two-port valve, air inlet two-port valve is simultaneously closed off,
Blake bottle is in air-tight state, gather ambient atmos, ambient atmos concentration is the initial concentration before culture；
(3) closed culture
Gas production two-port valve and connecting tube are disconnected, balance pipe is emptied, balance pipe is connected with air inlet two-port valve, open air inlet two-way
Valve, makes balance pipe be communicated with blake bottle, keeps air pressure in blake bottle；According to test requirements document, whole device is placed on corresponding conditionses
Under closed culture 1-24h；
(4) gas collecting
At the end of closed culture, gas production needle tubing is connected with gas production two-port valve, opens gas production two-port valve, repeatedly push-and-pull gas production pin
Pipe, fully to mix gas in blake bottle, gathers gas immediately.
2. in measuring cell according to claim 1 culture soil greenhouse gas emission method, it is characterised in that gas is adopted
After collection is finished, repeat step (2) after excluding the gas that soil incubation is produced in blake bottle, continues open according still further to step (1)
Culture, the sampling of different time sections is realized in repeat step (2)-(4)；Step (1), weighs and records soil sample with culture before culture
The gross weight of bottle, is supplemented because of the moisture of evaporation loss, using the injection with elongated syringe needle with gravimetric method in incubation per 2-4d
Device, to distilled water is supplemented in blake bottle, is reached consistent with the gross weight of record before culture by two-port valve.
3. in measuring cell according to claim 1 culture soil greenhouse gas emission method, it is characterised in that it is described
Sealing-plug is silica gel plug, and described air inlet pipe bottom and the distance of culture soil is the 1/8-1/5 of blake bottle height.
4. in measuring cell according to claim 1 culture soil greenhouse gas emission method, it is characterised in that it is described
Air collecting pipe bottom is the 1/5-1/4 of sealing-plug bottom and culture soil top layer apart from height with the distance of sealing-plug bottom, described
Blake bottle be the Flat bottom container such as triangular flask, wide-mouth bottle or narrow-mouthed bottle, material is plastics or glass.
5. in measuring cell according to claim 1 culture soil greenhouse gas emission method, it is characterised in that it is described
Air inlet pipe and air collecting pipe are polyfluortetraethylene pipe, and a diameter of 2-2.5mm is advisable with the thin mouth end airtight connection with two-port valve, interior
The preferred 0.8-1.2mm in aperture.Described two-port valve carries valve switch, the closure state for controlling different port.
6. in measuring cell according to claim 1 culture soil greenhouse gas emission method, it is characterised in that it is described
Ventilation house steward is hollow pipe, and hollow pipe includes two parallel straight tubes and two parallel vertical tubes, and two parallel vertical tubes are located at two and put down
Between row straight tube and connection form loop, branch pipe equi-spaced apart is arranged on two parallel straight tubes, the branch on two parallel straight tubes
Pipe intersects alternate setting.In order to place culture gas producing device, culture and the gas collecting of multiple blake bottles are once completed.
7. in measuring cell according to claim 1 culture soil greenhouse gas emission method, it is characterised in that it is described
Branch pipe quantity is 10-20, adjacent branch interval 10cm-20cm.
8. in measuring cell according to claim 1 culture soil greenhouse gas emission method, it is characterised in that gas is opened
Device preferably piston is closed, the material of hollow pipe, branch pipe and piston is glass；The material of flexible pipe and connecting tube is hollow hard
Plastics, two parallel straight tubes extend to two ends connection end is formed outside vertical tube respectively, and hollow pipe is fixed on support by connection end
On.
9. in measuring cell according to claim 1 culture soil greenhouse gas emission method, it is characterised in that hollow pipe
Support is fixed on by flask holder and cross clamp, set screw is provided with flask holder, for clamping hollow pipe, flask holder passes through
Set screw is fixed on cross clamp, and cross clamp is fixed on support by roll adjustment screw.
10. in measuring cell according to claim 1 culture soil greenhouse gas emission method, it is characterised in that ventilation
Time is 30s-3min, and closed incubation time is 2-10h.
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