CN102680662A - Device and method for synchronously determining soil substrate and gas emission - Google Patents
Device and method for synchronously determining soil substrate and gas emission Download PDFInfo
- Publication number
- CN102680662A CN102680662A CN2012101523816A CN201210152381A CN102680662A CN 102680662 A CN102680662 A CN 102680662A CN 2012101523816 A CN2012101523816 A CN 2012101523816A CN 201210152381 A CN201210152381 A CN 201210152381A CN 102680662 A CN102680662 A CN 102680662A
- Authority
- CN
- China
- Prior art keywords
- gas
- culture tank
- tank
- culture
- substrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000002689 soil Substances 0.000 title claims abstract description 49
- 239000000758 substrate Substances 0.000 title claims abstract description 46
- 238000000034 method Methods 0.000 title claims abstract description 27
- 239000007789 gas Substances 0.000 claims abstract description 94
- 239000011261 inert gas Substances 0.000 claims abstract description 21
- 238000004458 analytical method Methods 0.000 claims abstract description 4
- 238000001514 detection method Methods 0.000 claims abstract description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 14
- 230000008859 change Effects 0.000 claims description 13
- 230000001360 synchronised effect Effects 0.000 claims description 10
- 229910052757 nitrogen Inorganic materials 0.000 claims description 5
- 229910001220 stainless steel Inorganic materials 0.000 claims description 5
- 239000010935 stainless steel Substances 0.000 claims description 5
- 238000005070 sampling Methods 0.000 claims description 4
- 238000011534 incubation Methods 0.000 claims description 3
- 238000005452 bending Methods 0.000 claims description 2
- BGOFCVIGEYGEOF-UJPOAAIJSA-N helicin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=CC=C1C=O BGOFCVIGEYGEOF-UJPOAAIJSA-N 0.000 claims description 2
- 238000012360 testing method Methods 0.000 claims description 2
- 230000008569 process Effects 0.000 abstract description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 7
- 239000001301 oxygen Substances 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 230000004907 flux Effects 0.000 description 4
- 239000005431 greenhouse gas Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000007789 sealing Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- MWUXSHHQAYIFBG-UHFFFAOYSA-N Nitric oxide Chemical compound O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 3
- 238000007599 discharging Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000007774 longterm Effects 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 238000004088 simulation Methods 0.000 description 3
- 230000003068 static effect Effects 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 239000012774 insulation material Substances 0.000 description 2
- 238000009533 lab test Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 description 1
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000005264 electron capture Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 238000005206 flow analysis Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000010297 mechanical methods and process Methods 0.000 description 1
- 230000005226 mechanical processes and functions Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 229960003753 nitric oxide Drugs 0.000 description 1
- 235000019391 nitrogen oxide Nutrition 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000002277 temperature effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
Images
Landscapes
- Sampling And Sample Adjustment (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a device for synchronously determining a soil substrate and gas emission. The device mainly comprises inert gas and O2 sources, wherein the inert gas and O2 sources are connected to a gas inlet of a gas mixing tank via mass flow meters; the gas mixing tank is provided with a plurality of gas outlets; each gas outlet is connected with a gas inlet of each culture tank; each mass flow meter and each constant temperature tube are connected between each gas outlet and each culture tank in series; a gas outlet of each culture tank can be directly emptied or sampled to carry out gas detection and analysis; soil samples are contained in the culture tanks; a voltage analog quantity output module and a voltage analog quantity input module are installed on each mass flow meter and are connected to a computer; and each culture tank and each constant temperature tube are put in a constant temperature device. The invention also discloses a method for synchronously determining the soil substrate and gas emission by utilizing the device. By adopting the device and the method, variations of related gas emission and key substrate concentrations with time of the objects of study in the processes of nitrification and denitrification under the condition of specific environment can be illustrated.
Description
Technical field
The present invention relates to the device of a kind of synchronous mensuration soil substrate and gaseous emission.
The invention still further relates to and utilize said apparatus to measure the method for soil substrate and gaseous emission synchronously.
Background technology
Occurring in nature, the soil of all kinds of ecosystems constantly exchanges with atmosphere.This exchange process is the biomass geochemistry round-robin important step that is closely related with whole world change.Because Global climate change, greenhouse effect obtain increasing concern; The urban atmospheric pollution of frequently being paid close attention in recent years also forces the researcher to oxides of nitrogen (NO and NO
2Deng) further observe and study.Nitrification and denitrification are topmost greenhouse gases (CO in the soil
2, CH
4And N
2O) and the source, the important biomolecule face of land of chemically reactive gas NO, to its gaseous emission speed quantitatively and the understanding in depth of discharging factor of influence, become biomass geochemistry round-robin research focus over nearly 30 years.
Observation for the nitrification denitrification process gaseous emission is divided at present in the world:
1) open-air long-term location observation mainly uses the case method to measure the emission flux of gas of soil; Open-air long-term positioning experiment can reflect that the year border of greenhouse gas emission changes, and estimates annual total emission volumn.
2) lab simulation, the factor that controls environment is cultivated earth pillar in confined space, measures the gas concentration of headroom.Simulating lab test is because advantages such as controlled temperature, oxygen content, substrate content, so become quantitative examination emission flux of gas and the powerful measure of understanding the gaseous emission factor of influence in depth.Simulating lab test is divided into static method and dynamic method again.Static method is about to earth pillar and is positioned in the closed container, uses corresponding instrument to measure at a distance from a period of time with the syringe extracting gases.Dynamic method is about to earth pillar and is placed in the culture tank, and (mixed gas is He and O to purge headroom with mixed gas
2, also can use N
2Substitute He, oxygen content can be regulated), the gas flow of maintenance headroom is through intermittently measuring the gas concentration calculating soil greenhouse gas emission flux that mixed gas carries out.This method has kept the natural concentration gradient of soil-inferior atmospheric layer interface; The greenhouse gases that soil produces diffuse into headroom through gradient; More can reflect state of nature and the Soil Interface chemical state that soil atmosphere exchanges than static method, obtain application more and more widely.
Produce CO through the nitrification and denitrification process in the soil
2, CH
4, N
2The speed of O and NO receives the control of the available carbon nitrogen of microorganism concentration of substrate in the soil.Wherein carbon substrate mainly is dissolved organic carbon (DOC), and the nitrogen substrate mainly comprises nitrate nitrogen (NO
3 -) and ammonium nitrogen (NH
4 +).DOC through promoting growth of microorganism and activity, accelerates soil O on the other hand on the one hand through provide electronics and energy to promote denitrification for denitrifying microorganism
2Consumption, quicken the formation of soil anaerobic environment, promote the soil denitrification effect indirectly.NO
3 -Content not only influences soil denitrification speed, but also influences the composition of soil denitrification product, NH
4 +Content is the substrate of nitrification and the product of mineralising.Observation soil is under various substrate states and envirment factor; Gaseous emission speed and all gases relative scale; To understanding the associated gas discharging rule that various envirment factors produce soil nitrification and denitrification in depth and estimating significantly, also be the element task in global change research due field.
Summary of the invention
The object of the present invention is to provide the device of a kind of synchronous mensuration soil substrate and gaseous emission.
Another purpose of the present invention is to utilize said apparatus to measure the method for soil substrate and gaseous emission synchronously.
For realizing above-mentioned purpose, the device of synchronous mensuration soil substrate provided by the invention and gaseous emission mainly comprises:
Inert gas and O
2Source of the gas is connected to the air intake opening of gas mixing tank separately through a mass flowmeter;
The gas mixing tank has a plurality of gas outlets, and each gas outlet all respectively is connected to the air intake opening of a culture tank, is serially connected with mass flowmeter and constant warm tube between each gas outlet and the culture tank, and the gas detection analysis is carried out in direct emptying in the gas outlet of each culture tank or sampling;
Hold pedotheque in the culture tank;
Voltage analog output module and voltage analog load module are installed on each mass flowmeter, and said voltage analog output module and voltage analog load module are connected to computing machine;
Each culture tank places thermostat with each constant warm tube.
The device of said synchronous mensuration soil substrate and gaseous emission wherein, is equipped with gas gauge and constant pressure valve on the gas mixing tank.
The device of said synchronous mensuration soil substrate and gaseous emission, wherein, constant warm tube is for bending to spiral helicine stainless-steel tube.
The device of said synchronous mensuration soil substrate and gaseous emission wherein, for spiral is connected, is provided with O-ring seal between lid and the tank body between the lid of culture tank and the tank body.
The method of utilizing said apparatus to measure soil substrate and gaseous emission synchronously provided by the invention, key step includes:
A) NO in the mensuration soil substrate
3 -, NH
4 +And DOC, as soil substrate initial concentration;
B) adjustment inert gas and O
2Ratio and air inlet total flow, inert gas and O
2The air inlet total flow greater than the total flow of giving vent to anger;
C) computing machine is provided with and monitors each road inert gas and O simultaneously
2The flow value of gas;
D) the appearance gas of each culture tank of collection in interval time is measured NO, N respectively
2O, CO
2And CH
4
E) change condition of culture or cultivate a period of time after, take out the soil test NO in several culture tank
3 -, NH
4 +And DOC;
F) weight step C, D and E set up the corresponding curve that gaseous emission speed and concentration of substrate change in the incubation.
Described method, wherein, step C is the flow value through computer interface input gas, and shows the flow of each mass flowmeter simultaneously through this interface.
Described method, wherein, the change condition of culture among the step D is meant and changes cultivation temperature and change inert gas and O
2Ratio.
Described method, wherein, the cultivation a period of time among the step D is meant under the same terms and cultivates one to two day time.
The invention has the beneficial effects as follows:
1) the present invention utilizes mass flowmeter accurately to prepare gas componant; Mixing and form the pressure of stable and controllable in mixing gas tank; Through computing machine 12 all integrated Control Flow of mass flowmeter are also monitored in real time; The gas flow rate that guarantee to get into each culture tank is consistent, and water bath with thermostatic control cultivates temperature control and sealing function are provided, and makes each culture tank independent interference-free and environmental baseline is in full accord.
2) the present invention adopts multipath gas mixing and flow control to measure gaseous emission (NO, the N of the pedotheque in the culture tank under the same conditions
2O, CO
2And CH
4), and the pedotheque in material time point (after changing condition of culture or cultivating a period of time) takes out wherein 3 culture tank arbitrarily measures concentration of substrate, thereby monitors when realizing gaseous emission with the substrate variation.
Description of drawings
Fig. 1 is the device synoptic diagram that the present invention measures soil substrate and gaseous emission synchronously.
Fig. 2 is the structural representation of culture tank of the present invention.
Fig. 3 is provided with and monitors each road inert gas and O simultaneously with computing machine among the present invention
2Gas stream value interface.
Fig. 4 is a flow control software flow synoptic diagram among the present invention.
Embodiment
Device of the present invention is under the research certain environment condition; Getting in touch between gaseous emission ratio and flux and the base consumption provides strong experimental tool for explaining the single environment factor (like temperature, humidity, concentration of substrate, oxygen level etc.) to the influence of the soil nitrification gaseous emission relevant with denitrification.Device of the present invention can increase the substrate observation station through the passage expansion, is used for the multiple simulated experiments such as culturing sludge of microbe culture, wastewater treatment.
The present invention is in the adjustable and consistent state of temperature, oxygen content, soil moisture and initial substrate with abundant earth pillar, can realize long-term continuous analog experimental session, observes N
2O, NO, CH
4And CO
2Discharge over time, the while is used to measure substrate (NO at the earth pillar that different critical stages take out identical condition at random
3 -, NH
4 +And DOC), the rule of analytical gas discharging synthetically and envirment factor and substrate variation.
See also Fig. 1, device of the present invention comprises gas mixing portion, flow control part and constant temperature analog part.Wherein:
The gas mixing portion comprises highly purified inert gas (He, N
2Deng) and O
2Source of the gas, inert gas and O
2(range is respectively He:200mL min through mass flowmeter FC
-1, O
2: 40mLmin
-1, accuracy ± 1.5%) and get in the gas mixing tank 10 inert gas and O
2Through mass flowmeter FC regulation and control flow velocity ratio, make inert gas and O
2The gas of content (this ratio can change on demand), mixing in gas mixing tank 10.
In order to guarantee inert gas and O
2Mixed gas flow out from 12 gas outlets according to flow setting value is stable, the air intake opening of mass flowmeter FC and the pressure differential of gas outlet need remain between the 0.05-0.4MPa.Therefore should guarantee that there is certain pressure gas mixing tank 10 inside, promptly the air intake opening overall flow rate needs slightly greater than the gas outlet overall flow rate; Simultaneously, can not let pressure excessive and influence inert gas and O again
2Smooth and easy entering gas mixing tank 10.Therefore a tensimeter 11 and constant-voltage equipment 12 have been installed on gas mixing tank 10.Constant-voltage equipment 12 is one section airtight tubular container, in have piston to increase to move up with pressure, pressure accumulated certain pressure in gas mixing tank 10, the piston of constant pressure valve 12 can up be pushed away, spring then can be given opposition of piston.When pressure is shifted piston onto certain position when (a jar internal pressure is about 0.2MPa); Through tiny vent port of tube wall; Too much pressure will discharge from vent port, and piston is pushed back the position below the vent port again by spring, makes whole cavity recover closed state.This process is carried out repeatedly, guaranteed that the mixing gas tank is inner to keep malleation, and pressure fluctuates in small range, effectively guarantees the normal operation of all mass flowmeters.Be inert gas and the O that avoids Yin Wendu to cause simultaneously
2The pressure of mixed gas changes, and gas mixing tank 10 can be placed in the stay-warm case 13.Stay-warm case 13 is formed (known technology, not shown in the figures) by the intensive heating resistor band, insulation material, case and the temperature control indicator gauge that mix on the gas tank 10 of being wrapped in.The temperature of heating resistor band can be led to the setting of temperature control indicator gauge, and insulation material and case remain in the scope of setting temperature.So can make mixed gas reach a preset temperature earlier and get into culture tank through mass flowmeter again, can improve the accuracy of temperature controlling ability and mass flowmeter.
Flow control partly comprises 12 mass flowmeter FC that 12 gas outlets of gas mixing tank 10 connect one to one and install; And the voltage analog output module 20 and voltage analog load module 21 (as shown in Figure 4) that on 12 mass flowmeter FC, connect respectively, voltage analog output module 20 is connected to computing machine with voltage analog load module 21.Use analog output module to be known products (such as model ADAM-4021, grinding magnificent Advantech) in the present embodiment, the analog quantity load module is known products (such as model ADAM-4017, grinding magnificent Advantech).
The mass flowmeter that sell the open market is operated through the flowmeter display controller; The corresponding flowmeter display controller of mass flowmeter; Supply power through the flowmeter display controller; And carry out flow value through the knob on it and set input, show actual flow through the digital display tube that carries.In device of the present invention, if 12 corresponding 12 flowmeter display controller of mass flowmeter, very large space, and a large amount of power interface; When carrying out the setting of 12 tunnel flow value simultaneously, needing to regulate through manual knob not only waste time and energy, and error is big and can't monitor in real time.Therefore, the unified power supply of mass flowmeter that the present invention is all is by computer installation and quality monitoring flowmeter flow.(like Fig. 3 and shown in Figure 4) input flow rate setting value in the interface by computing machine; Convert the digital signal setting value into through computing machine and change 485 converters through the RS232 interface; By 485 bus transfer digital semaphore to voltage analog load modules 20; Converting the digital signal amount into corresponding d. c. voltage signal is input in the mass flowmeter; The corresponding d. c. voltage signal rreturn value signal of mass flowmeter output actual flow is converted into digital quantity turns back to computing machine again through 485 buses graphical interfaces relevant position through voltage analog output module 21, accomplishes operation and monitoring to mass flowmeter FC thus.The flow of 12 road mass flowmeters can set and show in the interface of computing machine simultaneously, and the gas condition that makes synchronously, controls quickly and accurately 12 culture tank becomes possibility, has greatly improved work efficiency and experimental reliability, and saved a large amount of spaces and funds.
The constant temperature analog part is made up of (among Fig. 1 with serial number 1,2 culture tank 16 12 sealings that are connected to 12 road mass flowmeter downstream, that processed by stainless steel ..., 12 indicate 12 culture tank).All respectively be connected in series a constant warm tube 14 between the air intake opening of the gas outlet of mass flowmeter and culture tank 16; Culture tank 16 places a thermostat 15 together with constant warm tube 14; The gas that makes gas mixing tank 10 is before getting into culture tank 16, and the temperature of gas is consistent with the temperature of thermostat 15.The temperature that thermostat 15 is not merely soil simulation provides accurate control, and plays sealing function, avoids internal gas to receive in the atmosphere composition interference measurements such as oxygen.Thermostat 15 of the present invention can adopt water-bath to carry out constant temperature.The present invention adopts 2 meters long 1/8 stainless-steel tubes made in Great Britain to bend to spiral fashion and places water-bath as constant warm tube, to enlarge the contact area of pipeline 14 and water-bath, increase its constant temperature effect.
The structure of culture tank 16 is as shown in Figure 2, and the top is a cover 121, with tank body 122 for being threaded, be provided with an O-ring seal 123 between cover 121 and the tank body 122 to strengthen the sealing in the culture tank 12.The upper end of tank body 122 respectively has an air intake opening 124 and gas outlet 125.The air intake opening 124 of each culture tank 16 respectively connects a constant warm tube 14, the gas outlet 125 direct emptying respectively of each culture tank 12, or use pertinent instruments sampling analysis (known technology, not shown in the figures).
Pedotheque in the culture tank 12 is directly to be placed in the culture tank 12 behind the cutting ring 126 taking-up pedotheques by sampling, tightens lid.Culture tank 12 is lain in the water bath with thermostatic control, regulate water bath with thermostatic control, i.e. the may command soil moisture.
During practical operation, measure the substrate (NO of soil earlier
3 -, NH
4 +And DOC), as initial substrate concentration.Measure different N O if desired
3 -, the DOC gradient emission status, can in earth pillar, add concentration known NO
3 -With the DOC mixed solution.Adjust inert gas (present embodiment is example with He) and O by design
2Total flow and oxygen proportion make He and O
2Total flow is a bit larger tham the total flow of each gas outlet.Flow value (general 5ml min through each road gas of computer control layout setting
-1).Because 12 road mass flowmeters use He to demarcate, as contain O
2Need convert according to the correction coefficient that the flowmeter instructions provides and set flow, actual flow and experimental design are consistent.In the actual mechanical process, certain hour is gathered appearance gas with syringe with the speed of charge flow rate at interval, and (6820Agilent Technologies Inc., Wilmington DE) measure N to use GC6820 respectively
2O (electron capture detector, ECD), CO
2And CH
4(flame ionization detector, FID); Appearance gas and N
2Mix the airbag (3L at least) of packing into, use nitrogen-oxide analyzer, (42i Thermo Environment Instrument Inc., USA) mensuration NO based on chemiluminescence principle.
Simulation key point (raising oxygen level variation etc. like temperature), perhaps interval certain hour (a day or two days) is got 3 soil in the culture tank at random and is done replicate determination, gets 20g soil after the native mixing in each cutting ring, with 100ml KCl solution (1M) lixiviate NO
3 -And NH
4 +, with 100ml deionized water lixiviate DOC, (Skalar, SA5000 Netherland) measure each item substrate content to use four-way flow analysis appearance.The present invention is an example with 12 culture tank, therefore can obtain the concentration of substrate of 3 points between culture period, adds that the initial sum end point has 5 concentration of substrate.If the more substrate points of needs between the tracking culture period can increase mass flowmeter and culture tank is expanded.
Said method of the present invention is set up the corresponding curve of gaseous emission speed and concentration of substrate variation in the complete whole incubation of reaction of a cover, for the research of denitrification nitrification mechanism provides strong instrument.
Claims (8)
1. device of measuring synchronously soil substrate and gaseous emission mainly comprises:
Inert gas and O
2Source of the gas is connected to the air intake opening of gas mixing tank separately through a mass flowmeter;
The gas mixing tank has a plurality of gas outlets, and each gas outlet all respectively is connected to the air intake opening of a culture tank, is serially connected with mass flowmeter and constant warm tube between each gas outlet and the culture tank, and the gas detection analysis is carried out in direct emptying in the gas outlet of each culture tank or sampling;
Hold pedotheque in the culture tank;
Voltage analog output module and voltage analog load module are installed on each mass flowmeter, and said voltage analog output module and voltage analog load module are connected to computing machine;
Each culture tank places thermostat with each constant warm tube.
2. according to the device of said synchronous mensuration soil substrate of claim 1 and gaseous emission, wherein, gas gauge and constant pressure valve are installed on the gas mixing tank.
3. according to the device of said synchronous mensuration soil substrate of claim 1 and gaseous emission, wherein, constant warm tube is for bending to spiral helicine stainless-steel tube.
4. according to the device of said synchronous mensuration soil substrate of claim 1 and gaseous emission, wherein, for spiral is connected, be provided with O-ring seal between lid and the tank body between the lid of culture tank and the tank body.
5. utilize the said device of claim 1 to measure the method for soil substrate and gaseous emission synchronously, key step includes:
A) NO in the mensuration soil substrate
3 -, NH
4 +And DOC, as soil substrate initial concentration;
B) adjustment inert gas and O
2Ratio and air inlet total flow, inert gas and O
2The air inlet total flow greater than the total flow of giving vent to anger;
C) computing machine is provided with and monitors each road inert gas and O simultaneously
2The flow value of mixed gas;
D) the appearance gas of each culture tank of collection in interval time is measured NO, N respectively
2O, CO
2And CH
4
E) change condition of culture or cultivate a period of time after, take out the soil test NO in 3 culture tank
3 -, NH
4 +And DOC;
F) weight step C, D and E set up the corresponding curve that gaseous emission speed and concentration of substrate change in the incubation.
6. method according to claim 5, wherein, step C is the flow value through computing machine flow control software operation interface input gas, and shows the flow of each mass flowmeter simultaneously through this interface.
7. method according to claim 5, wherein, the change condition of culture among the step D is meant and changes cultivation temperature and change inert gas and O
2Ratio.
8. method according to claim 5, wherein, the cultivation a period of time among the step D is meant under the same terms and cultivates one to two day time.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210152381.6A CN102680662B (en) | 2012-05-16 | 2012-05-16 | Device and method for synchronously determining soil substrate and gas emission |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210152381.6A CN102680662B (en) | 2012-05-16 | 2012-05-16 | Device and method for synchronously determining soil substrate and gas emission |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102680662A true CN102680662A (en) | 2012-09-19 |
| CN102680662B CN102680662B (en) | 2015-04-29 |
Family
ID=46812906
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210152381.6A Expired - Fee Related CN102680662B (en) | 2012-05-16 | 2012-05-16 | Device and method for synchronously determining soil substrate and gas emission |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102680662B (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104458921A (en) * | 2014-12-10 | 2015-03-25 | 中国科学院遗传与发育生物学研究所 | Gas emission determinator and method for determining trace nitrogen emission flux using same |
| CN104777271A (en) * | 2015-04-01 | 2015-07-15 | 裴丽欣 | Multifunctional dry and wet deposition trickling filtration testing system |
| CN106018025A (en) * | 2016-05-25 | 2016-10-12 | 河海大学 | Semiautomatic culture apparatus for determining denitrification potential of soil |
| CN106198927A (en) * | 2016-08-02 | 2016-12-07 | 天津百利种苗培育有限公司 | A kind of Herba Oenanthes Javanicae differentiates the method for soil toxic gas |
| CN106442934A (en) * | 2016-09-28 | 2017-02-22 | 中国科学院亚热带农业生态研究所 | Method and device for collecting nitrogen produced by soil denitrifying microorganisms |
| CN106932241A (en) * | 2017-02-09 | 2017-07-07 | 山东省农业科学院农业资源与环境研究所 | A kind of method of culture soil greenhouse gas emission in measuring cell |
| CN107918002A (en) * | 2017-11-10 | 2018-04-17 | 邵崇军 | A kind of device of on-line determination lower soil respiratory intensity |
| CN109557255A (en) * | 2018-11-15 | 2019-04-02 | 广东精科环境科技有限公司 | A kind of intelligence SOIL GAS detection method and system |
| CN110274800A (en) * | 2019-07-24 | 2019-09-24 | 中国科学院成都生物研究所 | A kind of soil incubation device and soil incubation and gas production method based on the device |
| WO2021056414A1 (en) * | 2019-09-27 | 2021-04-01 | 深圳先进技术研究院 | Multi-channel culture system having controllable oxygen concentrations |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19906872C2 (en) * | 1999-02-18 | 2003-07-17 | Karlsruhe Forschzent | Method for determining the nitrification and / or denitrification rate of soils and device therefor |
| CN2689231Y (en) * | 2003-07-23 | 2005-03-30 | 北京莱森电子技术有限公司 | Standard gas intelligent proportioning apparatus |
| CN1975411A (en) * | 2006-12-13 | 2007-06-06 | 中国科学院沈阳应用生态研究所 | Method for measuring N2O discharge of soil plant system |
| CN101876632A (en) * | 2009-04-28 | 2010-11-03 | 中国科学院沈阳应用生态研究所 | Method for measuring content of carbon and nitrogen in soil |
| CN102095827A (en) * | 2009-12-09 | 2011-06-15 | 中国科学院大气物理研究所 | System and method for measuring carbon dioxide, methane and nitrous oxide in air simultaneously |
-
2012
- 2012-05-16 CN CN201210152381.6A patent/CN102680662B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19906872C2 (en) * | 1999-02-18 | 2003-07-17 | Karlsruhe Forschzent | Method for determining the nitrification and / or denitrification rate of soils and device therefor |
| CN2689231Y (en) * | 2003-07-23 | 2005-03-30 | 北京莱森电子技术有限公司 | Standard gas intelligent proportioning apparatus |
| CN1975411A (en) * | 2006-12-13 | 2007-06-06 | 中国科学院沈阳应用生态研究所 | Method for measuring N2O discharge of soil plant system |
| CN101876632A (en) * | 2009-04-28 | 2010-11-03 | 中国科学院沈阳应用生态研究所 | Method for measuring content of carbon and nitrogen in soil |
| CN102095827A (en) * | 2009-12-09 | 2011-06-15 | 中国科学院大气物理研究所 | System and method for measuring carbon dioxide, methane and nitrous oxide in air simultaneously |
Non-Patent Citations (5)
| Title |
|---|
| RUI WANG, ETC.: "Measurement of N 2 , N 2 O, NO, and CO 2 Emissions from Soil with the Gas-Flow-Soil-Core Technique-SUPPORTING INFORMATION", 《ENVIRONMENTAL SCIENCE & TECHNOLOGY》 * |
| RUI WANG, ETC: "Measurement of N2, N2O, NO and CO2 emissions from soil with the gas-flow-soil-core technique", 《ENVIRONMENTAL SCIENCE & TECHNOLOGY》 * |
| RUI WANG，ETC.: "Characterization of the temporal dynamics of soil CO2 and N gas production (NO, N2O, N2) under varying environmental conditions", 《EGU GENERAL ASSEMBLY 2010》 * |
| 王跃思 等: "一台气相色谱仪同时测定陆地生态系统CO2、CH4 和N2O 排放", 《环境污染治理技术与设备》 * |
| 金春义 等: "内蒙古典型草原土壤N 2 、N 2 O、NO和CO 2 排放的研究——土柱培养实验", 《中国农学通报》 * |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104458921B (en) * | 2014-12-10 | 2016-04-06 | 中国科学院遗传与发育生物学研究所 | Gas discharging determinator and use it to measure the method for trace nitrogen gas emission flux |
| CN104458921A (en) * | 2014-12-10 | 2015-03-25 | 中国科学院遗传与发育生物学研究所 | Gas emission determinator and method for determining trace nitrogen emission flux using same |
| CN104777271A (en) * | 2015-04-01 | 2015-07-15 | 裴丽欣 | Multifunctional dry and wet deposition trickling filtration testing system |
| CN106018025B (en) * | 2016-05-25 | 2019-03-05 | 河海大学 | A kind of auto-manual system culture apparatus for the measurement of soil denitrification potentiality |
| CN106018025A (en) * | 2016-05-25 | 2016-10-12 | 河海大学 | Semiautomatic culture apparatus for determining denitrification potential of soil |
| CN106198927A (en) * | 2016-08-02 | 2016-12-07 | 天津百利种苗培育有限公司 | A kind of Herba Oenanthes Javanicae differentiates the method for soil toxic gas |
| CN106442934A (en) * | 2016-09-28 | 2017-02-22 | 中国科学院亚热带农业生态研究所 | Method and device for collecting nitrogen produced by soil denitrifying microorganisms |
| CN106932241A (en) * | 2017-02-09 | 2017-07-07 | 山东省农业科学院农业资源与环境研究所 | A kind of method of culture soil greenhouse gas emission in measuring cell |
| CN106932241B (en) * | 2017-02-09 | 2019-10-08 | 山东省农业科学院农业资源与环境研究所 | A kind of method of culture soil greenhouse gas emission in measuring cell |
| CN107918002A (en) * | 2017-11-10 | 2018-04-17 | 邵崇军 | A kind of device of on-line determination lower soil respiratory intensity |
| CN109557255A (en) * | 2018-11-15 | 2019-04-02 | 广东精科环境科技有限公司 | A kind of intelligence SOIL GAS detection method and system |
| CN110274800A (en) * | 2019-07-24 | 2019-09-24 | 中国科学院成都生物研究所 | A kind of soil incubation device and soil incubation and gas production method based on the device |
| WO2021056414A1 (en) * | 2019-09-27 | 2021-04-01 | 深圳先进技术研究院 | Multi-channel culture system having controllable oxygen concentrations |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102680662B (en) | 2015-04-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102680662A (en) | Device and method for synchronously determining soil substrate and gas emission | |
| Parajuli | Biogas measurement techniques and the associated errors | |
| Wang et al. | Effects of nitrate concentration on the denitrification potential of a calcic cambisol and its fractions of N 2, N 2 O and NO | |
| CN102586097B (en) | Device of continuously testing indoor soil microbial respiration | |
| Gruen et al. | Experimental investigation on the controls of clumped isotopologue and hydrogen isotope ratios in microbial methane | |
| Stephens et al. | Application of a differential fuel-cell analyzer for measuring atmospheric oxygen variations | |
| Parker et al. | Diel behavior of stable isotopes of dissolved oxygen and dissolved inorganic carbon in rivers over a range of trophic conditions, and in a mesocosm experiment | |
| CN105585129B (en) | A kind of simulation original position river channel ecology system nitrogen returns the device and method to become | |
| CN102618431B (en) | A kind of mass spectrometric closed photo bioreactor device of Kernel-based methods and frustule process of growth monitoring method | |
| CN2929723Y (en) | Dynamic gas distributor for multiple component expansion tube standard gases | |
| CN105675435A (en) | Method for carrying out in-situ measurement on respiration, nitrification and denitrification processes of soil | |
| CN105548438A (en) | Continuous high pressure oxidation experimental device and method for natural gas hydrate gas release | |
| Jiang et al. | Biogeochemical and physical controls on the evolution of dissolved inorganic carbon (DIC) and δ13CDIC in karst spring-waters exposed to atmospheric CO2 (g): insights from laboratory experiments | |
| Predotova et al. | Effects of cuvette surface material on ammonia‐, nitrous oxide‐, carbon dioxide‐, and methane‐concentration measurements | |
| CN102539795B (en) | Device for automatically testing gas generated from denitrification | |
| CN103894116B (en) | The large gas generating system of a kind of single mercury isotope | |
| CN201622258U (en) | Automatic oil gas concentration detector | |
| GB2530571A (en) | Gas analysis device | |
| Young et al. | Use of anaerobic respirometers for measuring gas production in toxicity and treatability tests | |
| CN101363825B (en) | Device for dynamically detecting carbon element loss of root system of plant | |
| CN102778523A (en) | Method for measuring and controlling capillary column flow of gas chromatograph | |
| Kotsyurbenko et al. | Protocols for measuring methanogenesis | |
| CN203733002U (en) | Device for adjusting water body pH (Potential of Hydrogen) by simulating climate warming | |
| KR100990368B1 (en) | Volume measuring device for gas produced by reaction of micro-organism in reaction tank and multi channel type automatic gas analyzing system comprising the same | |
| CN102175777B (en) | Measuring instrument and measuring method of greenhouse gases |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150429 |