CN208425281U - Plant culture and root exudates collection device - Google Patents
Plant culture and root exudates collection device Download PDFInfo
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- CN208425281U CN208425281U CN201821063217.7U CN201821063217U CN208425281U CN 208425281 U CN208425281 U CN 208425281U CN 201821063217 U CN201821063217 U CN 201821063217U CN 208425281 U CN208425281 U CN 208425281U
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- China
- Prior art keywords
- plant
- incubator
- collection device
- hole
- root exudates
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- Expired - Fee Related
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- 210000000416 exudates and transudate Anatomy 0.000 title claims abstract description 24
- 238000005192 partition Methods 0.000 claims abstract description 47
- 239000002689 soil Substances 0.000 claims abstract description 26
- 239000007788 liquid Substances 0.000 claims abstract description 16
- 238000007789 sealing Methods 0.000 claims description 32
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 17
- 229910052799 carbon Inorganic materials 0.000 claims description 17
- 229910002027 silica gel Inorganic materials 0.000 claims description 12
- 239000000741 silica gel Substances 0.000 claims description 12
- 229960001866 silicon dioxide Drugs 0.000 claims description 12
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 11
- 230000003287 optical effect Effects 0.000 claims description 5
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 239000012530 fluid Substances 0.000 claims description 4
- 230000008635 plant growth Effects 0.000 claims description 4
- 230000028327 secretion Effects 0.000 abstract description 6
- 238000011534 incubation Methods 0.000 abstract description 3
- 238000005259 measurement Methods 0.000 abstract description 3
- 241000196324 Embryophyta Species 0.000 description 29
- 239000000243 solution Substances 0.000 description 7
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 238000005286 illumination Methods 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 2
- 102000013275 Somatomedins Human genes 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N carbon dioxide Natural products O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000036531 allelopathy Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000006353 environmental stress Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000008638 plant developmental process Effects 0.000 description 1
- 230000002786 root growth Effects 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 238000005527 soil sampling Methods 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a kind of culture of plant and root exudates collection device, including incubator；The incubator is a U-shaped basin, it is vertically arranged with multiple partitions in the incubator, through-hole is provided on the multiple partition, the through-hole is multiple, the through-hole between the multiple partition adjacent separator is provided with soil liquid sampler in laterally staggered setting, the incubator.The utility model in incubator by being provided with multiple partitions for being provided with through-hole and through-hole is staggered, in plant incubation, through-hole between each partition forms plant lateral roots and grows boot section, so that after the completion of culture, the root system tissue of plant can be good at being separated with soil, the influence reduced to root system of plant disorganization to secretion concentration in root system of plant and soil is avoided, the precision of measurement result is improved.
Description
Technical field
The utility model belongs to for plant cultivation device, in particular to a kind of plant culture and root exudates collect dress
It sets.
Background technique
In plant growth and development process, root system be not only absorb and metabolic organ, from somatomedin absorb nutrient and
Moisture, and be powerful secretory, exudation or secretion proton, ion and a large amount of organic substance from somatomedin, i.e.,
Root exudates.Root exudates is in soil texture formation, living point of soil nutrient, plant nutrient absorption, environment-stress alleviation etc.
Aspect all plays a significant role, and the research to root exudates is plant nutrient, allelopathy, biological pollution stress, environment dirt
The important content of the research fields such as dye reparation, the common concern by domestic and foreign scholars.
Existing plant culture and root exudates collection device in, complete culture after, by plant from soil
It is more serious to root system of plant disorganization when separating, the concentration of root exudates is influenced, so that measurement result is missed
Difference is larger.
Utility model content
The purpose of the utility model is to provide one kind can reduce to the plant culture of root system of plant disorganization and root system
The device that secretion is collected.
To achieve the above object, technical solution provided by the utility model are as follows: a kind of culture of plant and root exudates are received
Acquisition means, including incubator；The incubator is a U-shaped basin, is vertically arranged with multiple partitions in the incubator, the multiple
Be provided with through-hole on partition, the through-hole be it is multiple, the through-hole between the multiple partition adjacent separator is in lateral friendship
Mistake is arranged, and is provided with soil liquid sampler in the incubator.
Further, the soil liquid sampler is arranged between the multiple partition adjacent separator.
Further, the longitudinal gap between the multiple partition adjacent separator is arranged at least two soil liquid and takes
Sample device.
Further, through-hole described on every three partitions of the multiple partition point-blank, and the straight line with
There are angle α between longitudinal axis, the angle α size is 45 °≤α≤75 °.
Further, the diameter of the through-hole is 2~5cm.
It further, further include sealing container and carbon isotope release device, the incubator and the carbon isotope are released
Device is put to be arranged in the sealing container；The lid for sealing U-shaped opening is provided on the incubator, on the lid
It is provided with the opening passed through for culture plant growth.
Further, the silicagel pad with hatch frame is provided at the aperture position of the lid, the silicagel pad
The stem part that hatch frame and plant grow the lid opening is closely fitted, the edge of the silicagel pad and the lid
The opening of body seals cooperation.
Further, the carbon isotope release device includes the first reactive tank, the second reactive tank, second reactive tank
Bottom is lower than first reaction tank bottom, and second reactive tank is connected by fluid pipeline with first reactive tank, institute
It states and is additionally provided with gas-liquid separator on the second reactive tank.
Further, lighting apparatus and dehumidifier are provided on the sealing container inner wall.
Further, be provided on the sealing container inner wall at least one gas concentration lwevel sensor, at least one
Temperature sensor, at least one humidity sensor and at least one optical sensor.
The utility model is include at least the following beneficial effects:
The utility model is logical on multiple partitions for being provided with through-hole and adjacent two partition by being provided in incubator
Hole is staggered, and in plant incubation, the through-hole between each partition forms plant lateral roots and grows boot section, so that
After the completion of culture, the root system tissue of plant can be good at being separated with soil, avoids and breaks to root system of plant tissue
It is bad, reduce the influence to secretion concentration in root system of plant and soil, improves the precision of measurement result.
Detailed description of the invention
Fig. 1 is the schematic diagram of the section structure that plant described in the utility model is cultivated and root exudates is collected.
Graphical sysmbol explanation:
1- sealing container, 2- incubator, 3- first partition, 4- second partition, 5- third partition, the sampling of the 6- soil liquid
Device, 7- cover board, the first reactive tank of 8-, the second reactive tank of 9-, 10- gas-liquid separator, 11- lighting apparatus, 12- carbon dioxide solubility
Inductor, 13- temperature sensor, 14- humidity sensor, 15- optical sensor；
301- first through hole, the second through-hole of 401-, 501- third through-hole, 701- silicagel pad.
Specific embodiment
The following describes the utility model in further detail with reference to the accompanying drawings, to enable those skilled in the art referring to explanation
Book text can be implemented accordingly.
It should be appreciated that such as " having ", "comprising" and " comprising " term used herein do not allot one or more
The presence or addition of a other elements or combinations thereof.
As shown in Figure 1, the utility model provides a kind of culture of plant and root exudates collection device, comprising:
Incubator 2, incubator 2 are a U-shaped basin, and multiple partitions are vertically arranged in incubator 2, are all provided on multiple partitions
Multiple through-holes are equipped with, multiple through-holes of adjacent separator are provided with soil liquid sampler in laterally staggered setting in incubator 2
6。
It further include having sealing container 1 and carbon isotope release device in the present embodiment.Incubator 2 and carbon isotope release
Device is arranged in sealing container 1, it is preferable that the diameter of multiple through-holes is 2~5cm on multiple partitions, is conducive to draw in this way
The plant lateral roots system for leading culture grows along through-hole；Through-hole on multiple every three partitions of partition point-blank, and straight line with
Longitudinal axis is in an angle α, and the size of angle need to guarantee for 45 °≤α≤75 °.
In the present embodiment, it is preferable that be symmetrically arranged with first partition 3, second partition 4 and third partition in incubator 1
5.It is evenly arranged with multiple first through hole 301 in first partition 3, multiple second through-holes 401 are evenly arranged on second partition 4,
Multiple third through-holes 501 are evenly arranged on third partition 5.First through hole 301 and the second through-hole 401 in laterally staggered setting,
Second through-hole 401 and third through-hole 501 are in laterally staggered setting.Further, first through hole 301, the second through-hole 401 and
Three through-holes 501, which are on same straight line, to be configured, and there are an angle αs between the straight line and partition longitudinal axis, need to guarantee 45 °
≤α≤75°.The distance between first partition 3 and second partition 4 are preferably 5~10cm, between second partition 4 and third partition 5
Distance be preferably 15~20cm, the side wall distance of third partition 5 and incubator 2 is preferably 20~30cm, and such distance is set
It sets to aloow and the secretion in the soil at plant main root different distance is collected and is studied in monitoring.
Incubator 2 is provided with the lid 7 for sealing U-shaped opening, and lid 7 is just to seal the U-shaped opening of incubator 2
It covers to be best, for the isolation inside and outside to incubator 2, avoids carbon from entering soil and absorbed to influence by root system
The collection of root exudates；The opening passed through for culture plant growth is provided on lid 7, the opening of lid 7 is provided with band
There is the silicagel pad 701 of hatch frame, the stem part that hatch frame and the plant of silicagel pad 701 grow the opening of lid 7 is adjacent to
The edge that cooperation, the edge of silicagel pad 701 and lid 7 are open, which seals, to be cooperated；In the present embodiment, it is preferable that cross
The center of lid 7 is arranged in shape opening silicagel pad 701.
Soil liquid sampler 6 is provided in incubator 2, more preferably, at least provided with two between adjacent separator two-by-two
A soil liquid sampler.
In the present embodiment, it is preferable that in first partition 3 and second partition 4, second partition 4 and third partition 5, third every
Soil liquid sampler 6 there are two being respectively set between 2 side wall of plate 5 and incubator, in longitudinal direction, two soil liquid samplers
6 distance is not less than 10cm, soil sampling can be carried out in the different distance of culture plant root growth in this way, so that testing number
According to more detailed, comparison is become apparent from.
Carbon isotope release device is arranged in sealing container 1, for generating carbon isotope comprising: the first reactive tank
8, the second reactive tank 9.The bottom of second reactive tank 9 is lower than the bottom of the first reactive tank 8, the first reactive tank 8 and the second reactive tank 9
It is connected by fluid pipeline, HCL solution can be contained in the first reactive tank 8, Na can be contained in the second reactive tank 913CO3Solution.
HCL solution can be flowed into the second reactive tank 9 by fluid pipeline from the first reactive tank 8 and Na13CO3Solution reaction generates carbon
Isotope, be additionally provided with gas-liquid separator 10 on the second reactive tank 9；It can be according to plant using the carbon isotope release device
In incubation to the demand of carbon isotope carry out reaction release carbon isotope number.
Lighting apparatus 11 is additionally provided on the inner wall of sealing container 1, in the present embodiment, it is preferable that the lighting apparatus 11
It is evenly distributed on 1 top inner wall of sealing container and upside with surrounding inner wall to be multiple, to give plant to provide illumination.
It is additionally provided with dehumidifier on the inner wall of sealing container 1, to reduce the humidity in sealing container 1.
It is additionally provided at least one gas concentration lwevel sensor 12 on the inner wall of sealing container 1, holds to detect sealing
Gas concentration lwevel in device 1.In the present embodiment, a gas concentration lwevel sensing 12 is preferably provided in the left side of sealing container 1
On inner wall.
At least one temperature sensor 13 is additionally provided on the inner wall of sealing container 1, to detect in sealing container 1 i.e.
Shi Wendu.In the present embodiment, a temperature sensor 13 is preferably provided in the inner left wall of sealing container 1.
At least one humidity sensor 14 is additionally provided on the inner wall of sealing container 1, it is wet in sealing container 1 to detect
Degree.In the present embodiment, a humidity sensor 14 is preferably provided in the inner left wall of sealing container 1.
At least one optical sensor 15 is additionally provided on the inner wall of sealing container 1, to detect the light in sealing container 1
Illumination.In the present embodiment, a temperature sensor 15 is preferably provided in the inner left wall of sealing container 1.
The specific embodiment of the present embodiment is as described below:
Soil is filled up in incubator 2, then plants plant into training by the cross-shaped aperture silicagel pad 701 on lid 7
It supports in case 2, covers lid 7, injection in sealing container 1 is eliminated into CO2Gas, injection length be 20~30min, guarantee sealing
Gas in container 1 is free of CO2.The HCL solution in the first reactive tank 8 is instilled later same to generate carbon in the second reactive tank 9
Position element13CO, and guarantee that its concentration is consistent with the concentration in natural environment, about 0.03%.Start lighting apparatus 11 simultaneously, remove
Wet device guarantees that the temperature in sealing container 1 is 25~28 degree, and humidity is 70~80%, 1700~2000 μm of ol/ of intensity of illumination
m2/s.During the cultivation process, multiple gas concentration lwevel sensors 12, multiple temperature sensors 13, multiple humidity sensors are utilized
Device 14 and multiple optical sensors 15 are monitored the intracorporal parameters of case, to the end of cultivating, stop monitoring.It is tied in culture
Shu Hou measures the secretions from plant roots at different location by the sampling of solution sampler 6, by incubator every
Plate and it through-hole be set easily on partition will cultivate root system of plant and separate with soil to guarantee to cultivate the root system of plant
Tissue preserves from, and so as to carry out complete sample detection to tissue root system, avoids root system and is damaged and give detection bring
Error.It after the soil in incubator is then toppled over mixing, air-dries, weighs, sampling, and detect the content of carbon in soil isotope
The collection of root exudates is carried out, simultaneously so as to late detection.
It is not only in the description and the implementation although the embodiments of the present invention have been disclosed as above
Listed utilization, it can be applied to various fields suitable for the present invention completely, for those skilled in the art,
Other modifications may be easily implemented, therefore without departing from the general concept defined in the claims and the equivalent scope, this reality
It is not limited to specific details and legend shown and described herein with novel.
Claims (10)
1. a kind of plant culture and root exudates collection device, which is characterized in that including incubator；
The incubator is a U-shaped basin, is vertically arranged with multiple partitions in the incubator, is provided on the multiple partition
Through-hole, the through-hole be it is multiple, the through-hole between the multiple partition adjacent separator is in laterally staggered setting, the culture
Soil liquid sampler is provided in case.
2. a kind of plant culture according to claim 1 and root exudates collection device, which is characterized in that the soil
Solution sampler is arranged between the multiple partition adjacent separator.
3. a kind of plant culture according to claim 2 and root exudates collection device, which is characterized in that the multiple
At least two soil liquid samplers are arranged in longitudinal gap between partition adjacent separator.
4. a kind of plant culture according to claim 1 and root exudates collection device, which is characterized in that the multiple
Point-blank, and there are angle αs between the straight line and longitudinal axis for the through-hole on every three partitions of partition, described
Angle α size is 45 °≤α≤75 °.
5. a kind of plant culture according to claim 1 and root exudates collection device, which is characterized in that the through-hole
Diameter be 2~5cm.
6. a kind of plant culture according to claim 1 and root exudates collection device, which is characterized in that further include close
Container and carbon isotope release device are sealed, the incubator and the carbon isotope release device are arranged in the sealing container
It is interior；It is provided with the lid for sealing U-shaped opening on the incubator, is provided on the lid and passes through for culture plant growth
Opening.
7. a kind of plant culture according to claim 6 and root exudates collection device, which is characterized in that the lid
Aperture position at be provided with the silicagel pad with hatch frame, the hatch frame of the silicagel pad and plant grow the lid
The stem part of opening is closely fitted, and the opening of the edge of the silicagel pad and the lid, which seals, to be cooperated.
8. a kind of plant culture according to claim 6 and root exudates collection device, which is characterized in that the carbon is same
The plain release device in position includes the first reactive tank, the second reactive tank, and second reaction tank bottom is lower than first reactive tank bottom
Portion, second reactive tank are connected by fluid pipeline with first reactive tank, are additionally provided with gas on second reactive tank
Liquid/gas separator.
9. a kind of plant culture according to claim 6 and root exudates collection device, which is characterized in that the sealing
Lighting apparatus and dehumidifier are provided on container inner wall.
10. a kind of plant culture according to claim 6 and root exudates collection device, which is characterized in that described close
At least one gas concentration lwevel sensor, at least one temperature sensor, at least one humidity are provided on envelope container inner wall
Sensor and at least one optical sensor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821063217.7U CN208425281U (en) | 2018-07-05 | 2018-07-05 | Plant culture and root exudates collection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821063217.7U CN208425281U (en) | 2018-07-05 | 2018-07-05 | Plant culture and root exudates collection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208425281U true CN208425281U (en) | 2019-01-25 |
Family
ID=65099422
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821063217.7U Expired - Fee Related CN208425281U (en) | 2018-07-05 | 2018-07-05 | Plant culture and root exudates collection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208425281U (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110672351A (en) * | 2019-11-08 | 2020-01-10 | 华南农业大学 | Device and method for collecting root exudates of gramineous plants |
| CN113702096A (en) * | 2021-09-18 | 2021-11-26 | 河南工程学院 | Plant rhizosphere box, rhizosphere box soil sample collecting device and collecting method |
| CN114088705A (en) * | 2021-10-15 | 2022-02-25 | 四川大学 | Plant underground ecological process observation system |
| CN114323788A (en) * | 2021-12-23 | 2022-04-12 | 华南师范大学 | Plant root exudate in-situ collection device and method for collecting plant root exudate by using same |
| CN114375695A (en) * | 2021-12-28 | 2022-04-22 | 四川农业大学 | In-situ collecting device and method for hygrophyte root exudates |
-
2018
- 2018-07-05 CN CN201821063217.7U patent/CN208425281U/en not_active Expired - Fee Related
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110672351A (en) * | 2019-11-08 | 2020-01-10 | 华南农业大学 | Device and method for collecting root exudates of gramineous plants |
| CN110672351B (en) * | 2019-11-08 | 2021-02-02 | 华南农业大学 | Device and method for collecting root exudates of gramineous plants |
| CN113702096A (en) * | 2021-09-18 | 2021-11-26 | 河南工程学院 | Plant rhizosphere box, rhizosphere box soil sample collecting device and collecting method |
| CN113702096B (en) * | 2021-09-18 | 2023-12-08 | 河南工程学院 | Rhizosphere box soil sample collection device and collection method |
| CN114088705A (en) * | 2021-10-15 | 2022-02-25 | 四川大学 | Plant underground ecological process observation system |
| CN114088705B (en) * | 2021-10-15 | 2023-08-04 | 四川大学 | Plant underground ecological process observation system |
| CN114323788A (en) * | 2021-12-23 | 2022-04-12 | 华南师范大学 | Plant root exudate in-situ collection device and method for collecting plant root exudate by using same |
| CN114323788B (en) * | 2021-12-23 | 2023-05-16 | 华南师范大学 | Plant root system secretion in-situ collection device and method for collecting plant root system secretion by adopting same |
| CN114375695A (en) * | 2021-12-28 | 2022-04-22 | 四川农业大学 | In-situ collecting device and method for hygrophyte root exudates |
| CN114375695B (en) * | 2021-12-28 | 2022-10-21 | 四川农业大学 | In-situ collecting device and method for hygrophyte root exudates |
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| CF01 | Termination of patent right due to non-payment of annual fee |
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