CN205861658U - The allelopathic test device of secretions from plant roots - Google Patents

The allelopathic test device of secretions from plant roots Download PDF

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Publication number
CN205861658U
CN205861658U CN201620818359.4U CN201620818359U CN205861658U CN 205861658 U CN205861658 U CN 205861658U CN 201620818359 U CN201620818359 U CN 201620818359U CN 205861658 U CN205861658 U CN 205861658U
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China
Prior art keywords
plant growth
growth case
allelopathic
case
test device
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Expired - Fee Related
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CN201620818359.4U
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Chinese (zh)
Inventor
左胜鹏
蒋小峰
王会梅
沈炎
丁莹
周守标
谢建春
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Anhui Normal University
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Anhui Normal University
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Abstract

The utility model discloses the allelopathic test device of a kind of secretions from plant roots, wherein, described allelopathic test device includes be connected donor plant growth case (1) arranged and recipient plant growth case (2), and separated by dividing plate (3) between described donor plant growth case and described recipient plant growth case, at least one through hole run through (4) it is provided with on described dividing plate, and it is coated with microporous membrane on described through hole, it is additionally provided with water inlet pipe (5) on described donor plant growth case, and described water inlet pipe is at least partially arranged at inside described donor plant growth case.By above-mentioned design, achieve component parts simple, root exudates need not preserve, be difficult to scatter and disappear, almost without cost, donor plant root exudates produces in situ, penetrates filter membrane, affects recipient plant g and D, and eliminate the restrictive condition of the factors such as nutrition, moisture, space, can intuitively observe the effect of the allelopathic effects of donor root exudates.

Description

The allelopathic test device of secretions from plant roots
Technical field
This utility model relates to allelopathic effects test device, in particular it relates to the allelopathic test dress of secretions from plant roots Put.
Background technology
Allelopathic effects (or allelopathy, allelopathy effect or allelopathic potential or counteract each other or allelopathy etc.): confession under directions Body plant passes through certain approach release characteristic chemical substance, also referred to as allelochemical, promotes or suppresses the growth of another plant and send out Educate.
Plant is typically by approach release allelochemicals such as root system secretion, misty rain leaching, volatilization, residuum decompositions.Plant Allelochemical can be divided into phenols (simple phenol, flavonoid), terpenoid (monoterpene, sterol), nitrogenous class (alkaloid, saponin), other times Biomass (coumarin) etc..Current allelopathic test is main payes attention to method and result, as utilized chemical extraction technology to extract wherein Active allelochemical, carry out indoor cultivation ware seed germination experiment;Or directly utilize the instrument such as GC-MS, GC-MS-MS, NMR Device analysis means, isolation identification allelochemical, or enrichment allelochemical, or buy allelochemical sterling, measure its allelopathic activity. But said method not only method is complex, and use cost is higher when practical operation.
Current immediate technology is broadly divided into three classes: the first kind is to collect root exudates device, as authorized publication number For CN101598638B, the device of a kind of collecting root exudate in situ, including incubator, support, illumination cultivation light, straight glass Glass pipe, peristaltic pump, nutrition bottle, resin column, surge flask and receiving flask etc., and this root exudates collection device, its constituting portion Part is many and complicated, and original root exudates volume is the most considerably less, easily misses through a lot of steps, i.e. enables collection, its amount Cannot meet a large amount of needs of experiment, and the root exudates collected needs certain shelf space and condition, cost is high;The Two classes are root exudates allelopathic assay methods, as authorized Publication No. CN102172128B, a kind of secretions from plant roots The bioassay method of allelopathic effects, utilizes donor plant to be grown on gelose medium, thus discharges accumulation allelochemical, removes Rear sowing or introduce a fine variety recipient plant, but the allelopathic method of this root exudates, use solid agar method to cultivate, often real Test loaded down with trivial details, introduce a fine variety recipient plant be difficult to survive, culture space limited, and be only applicable to the least seedling;3rd class is that root system divides The relevant apparatus and method of secretion, as authorized Publication No. CN100557407, the collection method of a kind of secretion from root system of plant in pot And collection device, authorize Publication No. CN101766100B, device for researching plant root exudate allelopathy and application thereof Method, this kind of collection device about root exudates and collection method, the research device of root exudates and application process etc., Its step is a lot, and component is complicated, a large amount of operations being not suitable in scientific experiments.
Therefore it provides a kind of component parts is simple, root exudates need not preserve, be difficult to scatter and disappear, almost without cost, donor Plant and recipient plant easily survive, donor plant root exudates produces in situ, penetrate filter membrane, affect recipient plant grow and Grow, applicable whole life cycle, and eliminate the restrictive condition of the factors such as nutrition, moisture, space, can intuitively observe donor root system The allelopathic effects of secretions, in greenhouse, laboratory, field etc. all can use, meet the practical situation in field, not only there is theory It is worth, and there is important production practical significance, it is simple to verify whether to there are autotoxicity, two species if appropriate for intercropping It is the problem that this utility model needs solution badly etc. the allelopathic test device of the secretions from plant roots of technical problem.
Utility model content
For above-mentioned prior art, the purpose of this utility model is to overcome conventional study root system of plant in prior art Feeling the method for potentiality or device not only component parts is many and complicated, during collecting root exudates, number of dropouts is big, it is impossible to full The full a large amount of needs tested, and the root exudates collected needs certain shelf space and condition, and cost is high, and tests numerous Trivial, introduce a fine variety recipient plant be difficult to survive, culture space limited, and the problems of a large amount of operations being not easy in scientific experiments, from And providing a kind of component parts simple, root exudates need not preserve, be difficult to scatter and disappear, almost without cost, donor plant and receptor Plant easily survives, and donor plant root exudates produces in situ, penetrates filter membrane, affects recipient plant g and D, is suitable for Whole life cycle, and eliminate the restrictive condition of the factors such as nutrition, moisture, space, can intuitively observe donor root exudates Allelopathic effects, in greenhouse, laboratory, field etc. all can use, meet the practical situation in field, not only there is theory value, and And there is important production practical significance, it is simple to verify whether to there are autotoxicity, two species if appropriate for technology such as intercroppings The allelopathic test device of the secretions from plant roots of problem.
To achieve these goals, this utility model provides the allelopathic test device of a kind of secretions from plant roots, its In, described allelopathic test device includes be connected the donor plant growth case arranged and recipient plant growth case, and described donor Separated by dividing plate between growth chamber and described recipient plant growth case, described dividing plate is provided with what at least one ran through It is coated with microporous membrane on through hole, and described through hole, described donor plant growth case is additionally provided with water inlet pipe, and described water inlet pipe It is at least partially arranged at inside described donor plant growth case.
Preferably, the bottom of described water inlet pipe is not more than 5cm apart from the distance of the bottom of described donor plant growth case.
Preferably, described microporous membrane is selected from nylon membrane and/or fibrous membrane, and the aperture of described microporous membrane is not more than 0.22 μ m。
Preferably, described donor plant growth case and described recipient plant growth case are also each detachably provided with gear Plate, and described donor plant growth case and described recipient plant growth case can be divided into and set from top to bottom by described baffle plate respectively He Xia district of Zhi Shang district, is arranged on the baffle plate on described donor plant growth case to be additionally provided with and matches with described water inlet pipe Pore, so that described water inlet pipe can run through described pore.
Preferably, described donor plant growth case and described recipient plant growth chamber interior wall are also vertically arranged with slide rail, The slide block matched with described slide rail it is provided with on described baffle plate.
Preferably, the bottom of described slide rail and the distance of the bottom of described donor plant growth case are more than described at least one The bottom of through hole grows the distance of the bottom of case with described donor plant, and the bottom of described slide rail grows case with described recipient plant The distance of bottom more than the distance of the bottom of bottom and the described recipient plant growth case of at least one described through hole.
Preferably, described slide rail is provided with at least one positioning lock locking mechanism, and the described mechanism in described location can lock Only described slide block sliding.
Preferably, described baffle plate is provided with at least one through substrate charging aperture.
According to technique scheme, donor plant growth case and recipient plant that this utility model is connected by setting are raw Long case, and above-mentioned two casing is separated by dividing plate, and then the through hole run through is set on dividing plate, so that plant in confession The allelochemical of the donor plant root system secretion in body growth chamber can be entered by through hole and be grown on recipient plant growth Recipient plant in case, meanwhile, in order to avoid in donor plant growth case, the microorganism etc. of breeding is planted by through hole entrance receptor Thing growth case, also covers microporous membrane on through hole, prevents passing through of microorganism etc. further, so avoid as far as possible extraneous its The impact on test of his factor, meanwhile, in order to further such that test operation is the simplest and quick, also by arranging water inlet pipe Directly to add water or nutritive water to root, supplement donor plant and the liquid manure of recipient plant normal growth needs, and This setup on the one hand moisture from bottom gradually ooze, do not worry that somatomedin upper surface hardens, form skinning, not shadow Ring plant growing;On the other hand, by water level lifting, root exudates is gradually driven by microporous membrane from donor plant growth case Enter to enter into recipient plant growth case, accelerate its testing time, improve testing efficiency.
Other feature and advantage of the present utility model will be described in detail in detailed description of the invention part subsequently.
Accompanying drawing explanation
Accompanying drawing is used to offer and is further appreciated by of the present utility model, and constitutes a part for description, with following Detailed description of the invention be used for explaining this utility model together, but be not intended that restriction of the present utility model.In the accompanying drawings:
Fig. 1 is the structural representation of the allelopathic test device of a kind of secretions from plant roots that this utility model provides.
Description of reference numerals
1-donor plant growth case 2-recipient plant growth case
3-dividing plate 4-through hole
5-water inlet pipe 6-baffle plate.
Detailed description of the invention
Below in conjunction with accompanying drawing, detailed description of the invention of the present utility model is described in detail.It should be appreciated that herein Described detailed description of the invention is merely to illustrate and explains this utility model, is not limited to this utility model.
In this utility model, in the case of illustrating on the contrary, " bottom, upper and lower, inwall and bottom " etc. is included in The noun of locality in term only represents this term orientation under normal service condition, or is the custom that skilled artisan understands that Claim, and be not construed as the restriction to this term.
This utility model provides the allelopathic test device of a kind of secretions from plant roots, wherein, as it is shown in figure 1, described Allelopathic test device includes be connected the donor plant growth case 1 arranged and recipient plant growth case 2, and described donor plant is raw Being separated by dividing plate 3 between long case 1 and described recipient plant growth case 2, described dividing plate 3 is provided with at least one runs through is logical It is coated with microporous membrane on hole 4, and described through hole 4, described donor plant growth case 1 is additionally provided with water inlet pipe 5, and described water inlet It is internal that pipe 5 is at least partially arranged at described donor plant growth case 1.
Donor plant growth case 1 and recipient plant that this utility model is connected by setting grow case 2, and by above-mentioned two Individual casing is separated by dividing plate 3, and then arranges the through hole 4 run through on dividing plate 3, grows case so that plant in donor plant The allelochemical of the donor plant root system secretion in 1 can enter the receptor being grown in recipient plant growth case 2 by through hole 4 Plant, meanwhile, in order to avoid in donor plant growth case 1, the microorganism etc. of breeding enters recipient plant growth case by through hole 4 2, on through hole 4, also cover microporous membrane, prevent passing through of microorganism etc. further, and then avoid other factors extraneous as far as possible Impact on test, meanwhile, in order to further such that test operation is the simplest and quick, also by arranging, water inlet pipe 5 is straight Connect and water or nutritive water are added to root, supplement donor plant and the liquid manure of recipient plant normal growth needs, and this Set-up mode on the one hand moisture from bottom gradually ooze, do not worry that somatomedin upper surface hardens, form skinning, do not affect and plant Thing grows;On the other hand, by water level lifting, root exudates is gradually driven in by microporous membrane from donor plant growth case 1 Enter into recipient plant growth case 2, accelerate its testing time, improve testing efficiency.
By above-mentioned set-up mode, first can add somatomedin in donor plant growth case 1, then donor be planted Thing is introduced a fine variety in above-mentioned donor plant growth case 1 so that is grown on the donor plant synthesis in donor plant growth case 1, produces And discharge allelopathic plant, similarly, in recipient plant growth case 2, add somatomedin, then recipient plant is introduced a fine variety in upper State in recipient plant growth case 2, by keeping the skin wet in water inlet pipe 5, drive allelochemical to migrate to recipient plant growth case 2 In, and act on the recipient plant of normal growth, and then carry out by directly observing or sample in recipient plant growth case 2 The mensuration of allelopathic effects, not only step is simple, and visual effect is good, and is easy to numerous studies.
Certainly, the volume of donor plant growth case 1 and recipient plant growth case 2 can select according to actual needs, Such as, when in view of the effect of spacial influence, donor plant can be grown case 1 and recipient plant growth case 2 is set to greatly Little identical.Certainly, this utility model is not limited thereto, and tester can be adjusted according to actual needs and select.
Certainly, one of the present utility model preferred embodiment in, can to the greatest extent may be used in order to ensure the water in water inlet pipe 5 Can arrive root position, the bottom of described water inlet pipe 5 distance apart from the bottom of described donor plant growth case 1 can be further It is defined to no more than 5cm.
Here microporous membrane can be microporous filter membrane type commonly used in the art, and material can enter according to actual needs Row selects, such as, one of the present utility model preferred embodiment in, described microporous membrane can selected from nylon membrane and/or Fibrous membrane, in order to reduce the impact of microorganism etc. in donor plant growth case 1 further, the aperture of described microporous membrane can enter one Step is chosen as no more than 0.22 μm.
Certainly, so that somatomedin can effectively be stored, it is to avoid the external environment pollution to somatomedin Deng, described donor plant growth case 1 and described recipient plant growth case 2 are also each detachably provided with baffle plate 6, and described Described donor plant growth case 1 and described recipient plant growth case 2 can be divided into arrange from top to bottom upper by baffle plate 6 respectively He Xia district of district, is arranged on the baffle plate 6 on described donor plant growth case 1 and is additionally provided with the pipe matched with described water inlet pipe 5 Hole, so that described water inlet pipe 5 can run through described pore.Somatomedin can be placed in the lower district that described baffle plate 6 is separated to form.
In order to the difference of the difference according to plant or research type carrys out the consumption of further growth regulation medium, in this reality With in novel a kind of embodiment being more highly preferred to, described donor plant growth case 1 and described recipient plant grow case 2 inwall On be also vertically arranged with slide rail, described baffle plate 6 is provided with the slide block matched with described slide rail.
Certainly, from somatomedin, recipient plant growth case is entered by through hole 4 to make allelochemical be more convenient for directly 2, be greatly improved testing efficiency, one of the present utility model preferred embodiment in, the bottom of described slide rail and described confession The distance of the bottom of body growth chamber 1 is more than the bottom of at least one described through hole 4 and the end of described donor plant growth case 1 The distance of end, the bottom of described slide rail is more than at least one described through hole 4 with the distance of the bottom of described recipient plant growth case 2 The distance of bottom of bottom and described recipient plant growth case 2.Certainly, the top of somatomedin here is preferably more than logical The bottom in hole 4.
In a kind of embodiment being more highly preferred to of the present utility model, so that can be according to the vegetation type of plantation And test-types carrys out the consumption of growth regulation medium, described slide rail is provided with at least one positioning lock locking mechanism, and described fixed The described mechanism in position can slide block sliding described in locking.Thus the size in lower district is regulated by the sliding of slide block.
In order to be easy to add somatomedin further, in a kind of embodiment being more highly preferred to, described baffle plate 6 also may be used To be provided with at least one through substrate charging aperture.
Preferred implementation of the present utility model is described in detail above in association with accompanying drawing, but, this utility model does not limit Detail in above-mentioned embodiment, in technology concept of the present utility model, can be to skill of the present utility model Art scheme carries out multiple simple variant, and these simple variant belong to protection domain of the present utility model.
It is further to note that each the concrete technical characteristic described in above-mentioned detailed description of the invention, at not lance In the case of shield, can be combined by any suitable means, in order to avoid unnecessary repetition, this utility model is to respectively Plant possible compound mode to illustrate the most separately.
Additionally, combination in any can also be carried out, as long as it is not disobeyed between various different embodiment of the present utility model Carrying on the back thought of the present utility model, it should be considered as content disclosed in the utility model equally.

Claims (8)

1. the allelopathic test device of a secretions from plant roots, it is characterised in that described allelopathic test device includes being connected Donor plant growth case (1) arranged and recipient plant grow case (2), and described donor plant grows case (1) and described receptor Separated by dividing plate (3) between growth chamber (2), described dividing plate (3) is provided with at least one through hole run through (4), and It is coated with microporous membrane on described through hole (4), described donor plant growth case (1) is additionally provided with water inlet pipe (5), and described water inlet It is internal that pipe (5) is at least partially arranged at described donor plant growth case (1).
Allelopathic the most according to claim 1 test device, it is characterised in that the bottom of described water inlet pipe (5) is apart from described The distance of the bottom of donor plant growth case (1) is not more than 5cm.
Allelopathic the most according to claim 1 and 2 test device, it is characterised in that described microporous membrane selected from nylon membrane and/or Fibrous membrane, and the aperture of described microporous membrane is not more than 0.22 μm.
Allelopathic the most according to claim 1 and 2 test device, it is characterised in that described donor plant growth case (1) and institute State in recipient plant growth case (2) and be also each detachably provided with baffle plate (6), and described baffle plate (6) can be by described donor Growth chamber (1) and described recipient plant growth case (2) are divided into Shang Qu and Xia Qu arranged from top to bottom respectively, are arranged at It is additionally provided with, on baffle plate (6) on described donor plant growth case (1), the pore matched with described water inlet pipe (5), so that Described water inlet pipe (5) can run through described pore.
Allelopathic the most according to claim 4 test device, it is characterised in that described donor plant growth case (1) and described Also it is vertically arranged with slide rail on recipient plant growth case (2) inwall, described baffle plate (6) is provided with and matches with described slide rail Slide block.
Allelopathic the most according to claim 5 test device, it is characterised in that the bottom of described slide rail and described donor plant The distance of the bottom of growth case (1) is more than the bottom of at least one described through hole (4) and the end of described donor plant growth case (1) The distance of end, the bottom of described slide rail and the distance of the bottom of described recipient plant growth case (2) are described logical more than at least one The bottom in hole (4) grows the distance of the bottom of case (2) with described recipient plant.
Allelopathic the most according to claim 5 test device, it is characterised in that be provided with at least one location on described slide rail Lockable mechanism, and the described mechanism in described location can slide block sliding described in locking.
Allelopathic the most according to claim 4 test device, it is characterised in that described baffle plate is provided with at least one on (6) Through substrate charging aperture.
CN201620818359.4U 2016-08-01 2016-08-01 The allelopathic test device of secretions from plant roots Expired - Fee Related CN205861658U (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114208464A (en) * 2021-12-14 2022-03-22 华南农业大学 Arbuscular mycorrhizal fungi application device and method capable of recycling fertilizer
CN114631446A (en) * 2022-03-31 2022-06-17 甘肃农业大学 Allelopathy research device combining volatilization and leaching modes

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114208464A (en) * 2021-12-14 2022-03-22 华南农业大学 Arbuscular mycorrhizal fungi application device and method capable of recycling fertilizer
CN114208464B (en) * 2021-12-14 2023-01-31 华南农业大学 Arbuscular mycorrhizal fungi application device and method capable of recycling fertilizer
CN114631446A (en) * 2022-03-31 2022-06-17 甘肃农业大学 Allelopathy research device combining volatilization and leaching modes

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